| Id |
Subject |
Object |
Predicate |
Lexical cue |
| T1 |
0-160 |
Sentence |
denotes |
Cadmium-mediated activation of the metal response element in human neuroblastoma cells lacking functional metal response element-binding transcription factor-1. |
| T2 |
161-364 |
Sentence |
denotes |
Metal response element-binding transcription factor-1 (MTF-1) binds specifically to metal response elements (MREs) and transactivates metallothionein (MT) gene expression in response to zinc and cadmium. |
| T3 |
365-599 |
Sentence |
denotes |
This investigation contrasts the mechanism of mouse MT gene (mMT-I) promoter activation by cadmium and zinc in IMR-32 human neuroblastoma cells to determine whether MTF-1 binding to the MRE is necessary for activation by these metals. |
| T4 |
600-718 |
Sentence |
denotes |
Cadmium activated a mMT-1 promoter (-150 base pairs) luciferase reporter 20-25-fold through a MRE-dependent mechanism. |
| T5 |
719-788 |
Sentence |
denotes |
In contrast, zinc had little effect on the mMT-1 luciferase reporter. |
| T6 |
789-900 |
Sentence |
denotes |
IMR-32 cells lacked MRE binding activity, and treatment with zinc in vitro or in vivo did not generate a MTF-1. |
| T7 |
901-965 |
Sentence |
denotes |
MRE complex, suggesting that IMR-32 cells lack functional MTF-1. |
| T8 |
966-1077 |
Sentence |
denotes |
Overexpression of mMTF-1 regenerated a zinc-mediated induction of the MRE without affecting cadmium activation. |
| T9 |
1078-1183 |
Sentence |
denotes |
Because no other transition metals tested activated the MRE, this effect appeared to be cadmium-specific. |
| T10 |
1184-1403 |
Sentence |
denotes |
These data demonstrate that in IMR-32 human neuroblastoma cells, zinc and cadmium can use independent mechanisms for activation of the mMT-I promoter and cadmium-mediated MRE activation is independent of MTF-1 and zinc. |
