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Id Subject Object Predicate Lexical cue
T50 0-7 Sentence denotes Results
T4493 9-56 Sentence denotes Inhibition of Tumor Development in CCR5−/− Mice
T51 9-56 Sentence denotes Inhibition of Tumor Development in CCR5−/− Mice
T4494 57-145 Sentence denotes The effect of CCR5 in tumor development was investigated using CCR5+/+ and CCR5−/− mice.
T52 57-145 Sentence denotes The effect of CCR5 in tumor development was investigated using CCR5+/+ and CCR5−/− mice.
T4495 146-238 Sentence denotes B16-F0 melanoma cells were inoculated subcutaneously into CCR5+/+ and CCR5−/− mice (n = 20).
T53 146-238 Sentence denotes B16-F0 melanoma cells were inoculated subcutaneously into CCR5+/+ and CCR5−/− mice (n = 20).
T4496 239-278 Sentence denotes Tumor growth was monitored for 31 days.
T54 239-278 Sentence denotes Tumor growth was monitored for 31 days.
T4497 279-366 Sentence denotes Tumor growth in the CCR5−/− mice was significantly lower than that in the CCR5+/+ mice.
T55 279-366 Sentence denotes Tumor growth in the CCR5−/− mice was significantly lower than that in the CCR5+/+ mice.
T4498 367-588 Sentence denotes The tumor growth in the CCR5−/− mice was reduced to 19.1%, whereas the tumor growth in the CCR5+/+ mice was only reduced to 24.3%, in both tumor weight (Figure 1A and 1B) and tumor volume, respectively (Figure 1A and 1C).
T56 367-588 Sentence denotes The tumor growth in the CCR5−/− mice was reduced to 19.1%, whereas the tumor growth in the CCR5+/+ mice was only reduced to 24.3%, in both tumor weight (Figure 1A and 1B) and tumor volume, respectively (Figure 1A and 1C).
T4499 589-639 Sentence denotes These results correlated with mice survival rates.
T57 589-639 Sentence denotes These results correlated with mice survival rates.
T4500 640-801 Sentence denotes There was a significant difference in survival rates between CCR5−/− mice (75% of starting time) and CCR5+/+ mice (15%) at the end of the experiment (Figure 1D).
T58 640-801 Sentence denotes There was a significant difference in survival rates between CCR5−/− mice (75% of starting time) and CCR5+/+ mice (15%) at the end of the experiment (Figure 1D).
T4501 802-893 Sentence denotes There was a significant difference in survival rates between CCR5−/− mice and CCR5+/+ mice.
T59 802-893 Sentence denotes There was a significant difference in survival rates between CCR5−/− mice and CCR5+/+ mice.
T4502 894-1001 Sentence denotes At the end of the experiment, 75% of the CCR5−/− mice survived while only 15% of the CCR5+/+ mice survived.
T60 894-1001 Sentence denotes At the end of the experiment, 75% of the CCR5−/− mice survived while only 15% of the CCR5+/+ mice survived.
T4503 1002-1088 Sentence denotes Subcutaneous tumors were harvested from the sacrificed mice 31 days after inoculation.
T61 1002-1088 Sentence denotes Subcutaneous tumors were harvested from the sacrificed mice 31 days after inoculation.
T4504 1089-1259 Sentence denotes The immunohistochemical analysis of tumor sections, stained with H&E and for proliferation antigen PCNA, revealed greater inhibition of tumor cell growth in CCR5−/− mice.
T62 1089-1259 Sentence denotes The immunohistochemical analysis of tumor sections, stained with H&E and for proliferation antigen PCNA, revealed greater inhibition of tumor cell growth in CCR5−/− mice.
T4505 1260-1426 Sentence denotes The histologic findings after H&E staining indicated that the tumor tissues of the CCR5−/− mice, but not those of the CCR5+/+ mice, contained large areas of necrosis.
T63 1260-1426 Sentence denotes The histologic findings after H&E staining indicated that the tumor tissues of the CCR5−/− mice, but not those of the CCR5+/+ mice, contained large areas of necrosis.
T4506 1427-1506 Sentence denotes PCNA reactive cells were significantly reduced in the CCR5−/− mice (Figure 1E).
T64 1427-1506 Sentence denotes PCNA reactive cells were significantly reduced in the CCR5−/− mice (Figure 1E).
T65 1507-1599 Sentence denotes 10.1371/journal.pone.0033747.g001 Figure 1 Inhibition of tumor development in CCR5−/− mice.
T17206 1551-1599 Sentence denotes Inhibition of tumor development in CCR5−/− mice.
T17207 1600-1640 Sentence denotes A–C, Tumor images, volumes, and weights.
T66 1600-1640 Sentence denotes A–C, Tumor images, volumes, and weights.
T17208 1641-1745 Sentence denotes B16 melanoma cells (5×105 cells/mouse) were inoculated s.c. into CCR5+/+ mice and CCR5−/− mice (n = 20).
T67 1641-1745 Sentence denotes B16 melanoma cells (5×105 cells/mouse) were inoculated s.c. into CCR5+/+ mice and CCR5−/− mice (n = 20).
T17209 1746-1846 Sentence denotes Tumor volumes were measured every day and tumor weights were measured at study termination (Day 31).
T68 1746-1846 Sentence denotes Tumor volumes were measured every day and tumor weights were measured at study termination (Day 31).
T17210 1847-1947 Sentence denotes The CCR5−/− mice had a significant reduction in tumor growth and volume as compared to CCR5+/+ mice.
T69 1847-1947 Sentence denotes The CCR5−/− mice had a significant reduction in tumor growth and volume as compared to CCR5+/+ mice.
T17211 1948-2050 Sentence denotes The results are expressed as mean ± SD. * indicates significant difference from CCR5+/+ mice (P<0.05).
T70 1948-2050 Sentence denotes The results are expressed as mean ± SD. * indicates significant difference from CCR5+/+ mice (P<0.05).
T17212 2051-2164 Sentence denotes D, The CCR5−/− mice demonstrated significantly higher survival rates compared to CCR5+/+ mice (by Log-rank Test).
T71 2051-2164 Sentence denotes D, The CCR5−/− mice demonstrated significantly higher survival rates compared to CCR5+/+ mice (by Log-rank Test).
T72 2165-2167 Sentence denotes E.
T17213 2165-2294 Sentence denotes E. Tumor sections were analyzed by H&E stain and expression of proliferating cell nuclear antigen (PCNA) by immunohistochemistry.
T73 2168-2294 Sentence denotes Tumor sections were analyzed by H&E stain and expression of proliferating cell nuclear antigen (PCNA) by immunohistochemistry.
T17214 2295-2409 Sentence denotes Data are means ± S.D. of four experimental animals. * indicates significant difference from CCR5+/+ mice (P<0.05).
T74 2295-2409 Sentence denotes Data are means ± S.D. of four experimental animals. * indicates significant difference from CCR5+/+ mice (P<0.05).
T17215 2410-2435 Sentence denotes Scale bar indicates 50 m.
T75 2410-2435 Sentence denotes Scale bar indicates 50 m.
T5418 2437-2495 Sentence denotes Decrease of NF-κB Activity in Tumor Tissue of CCR5−/− Mice
T76 2437-2495 Sentence denotes Decrease of NF-κB Activity in Tumor Tissue of CCR5−/− Mice
T17744 2437-2623 Sentence denotes Decrease of NF-κB Activity in Tumor Tissue of CCR5−/− Mice The activation of NF-κB plays a critical role in cancer cell survival, especially melanoma cancer cells and in CCR5 expression.
T5419 2496-2623 Sentence denotes The activation of NF-κB plays a critical role in cancer cell survival, especially melanoma cancer cells and in CCR5 expression.
T77 2496-2623 Sentence denotes The activation of NF-κB plays a critical role in cancer cell survival, especially melanoma cancer cells and in CCR5 expression.
T5420 2624-2827 Sentence denotes To evaluate whether the tumor inhibition was related with the inactivation of NF-κB in melanoma, the DNA binding activity of NF-κB was determined by electromobility shift assay (EMSA) in melanoma tissue.
T78 2624-2827 Sentence denotes To evaluate whether the tumor inhibition was related with the inactivation of NF-κB in melanoma, the DNA binding activity of NF-κB was determined by electromobility shift assay (EMSA) in melanoma tissue.
T5421 2828-2935 Sentence denotes The constitutively activated DNA binding activity was reduced in the CCR5−/− mice tumor tissue (Figure 2A).
T79 2828-2935 Sentence denotes The constitutively activated DNA binding activity was reduced in the CCR5−/− mice tumor tissue (Figure 2A).
T5422 2936-3115 Sentence denotes Moreover, the translocation of p50 and p-p65 into the nucleus through the inhibition of phosphorylation of IκB was also prevented in the tumor tissues of CCR5−/− mice (Figure 2B).
T80 2936-3115 Sentence denotes Moreover, the translocation of p50 and p-p65 into the nucleus through the inhibition of phosphorylation of IκB was also prevented in the tumor tissues of CCR5−/− mice (Figure 2B).
T5423 3116-3269 Sentence denotes Immunofluorescence analysis also confirmed that the intensity of nuclear staining for p50 had decreased in the tumor tissues of CCR5−/− mice (Figure 2C).
T81 3116-3269 Sentence denotes Immunofluorescence analysis also confirmed that the intensity of nuclear staining for p50 had decreased in the tumor tissues of CCR5−/− mice (Figure 2C).
T82 3270-3373 Sentence denotes 10.1371/journal.pone.0033747.g002 Figure 2 Decrease of NF-κB activity in tumor tissue of CCR5−/− mice.
T17745 3374-3543 Sentence denotes A, The DNA binding activity of NF-κB was determined in the nuclear extracts of the CCR5−/− mice and CCR5+/+ mice tumor tissues by EMSA described in material and methods.
T83 3374-3543 Sentence denotes A, The DNA binding activity of NF-κB was determined in the nuclear extracts of the CCR5−/− mice and CCR5+/+ mice tumor tissues by EMSA described in material and methods.
T17746 3544-3698 Sentence denotes B, Expression of p50 and p65 phosphorylation in nuclear extracts (NE), and IκB and IκB phosphorylation in the cytosol (CE) determined by Western blotting.
T84 3544-3698 Sentence denotes B, Expression of p50 and p65 phosphorylation in nuclear extracts (NE), and IκB and IκB phosphorylation in the cytosol (CE) determined by Western blotting.
T17747 3699-3849 Sentence denotes C, Immunolfluorescence analysis of p50 confirmed that the intensities of nuclear staining for p50 were decreased in the tumor tissues of CCR5−/− mice.
T85 3699-3849 Sentence denotes C, Immunolfluorescence analysis of p50 confirmed that the intensities of nuclear staining for p50 were decreased in the tumor tissues of CCR5−/− mice.
T17748 3850-3964 Sentence denotes Data are means ± S.D. of four experimental animals. * indicates significant difference from CCR5+/+ mice (p<0.05).
T86 3850-3964 Sentence denotes Data are means ± S.D. of four experimental animals. * indicates significant difference from CCR5+/+ mice (p<0.05).
T17749 3965-3991 Sentence denotes Scale bar indicates 50 µm.
T87 3965-3991 Sentence denotes Scale bar indicates 50 µm.
T5957 3993-4102 Sentence denotes Induction of Apoptotic Cell Death and Inhibition of the Expression of Anti-apoptotic Proteins in CCR5−/− Mice
T88 3993-4102 Sentence denotes Induction of Apoptotic Cell Death and Inhibition of the Expression of Anti-apoptotic Proteins in CCR5−/− Mice
T18203 3993-5246 Sentence denotes Induction of Apoptotic Cell Death and Inhibition of the Expression of Anti-apoptotic Proteins in CCR5−/− Mice To see whether inhibition of NF-κB activity results in the induction of apoptotic cell death and expression of NF-κB target apoptotic cell death regulatory protein, the levels of apoptotic cell death and expression of apoptotic cell death regulatory proteins were assessed. Apoptotic cell death in the tumor sections were evaluated in the CCR5+/+ and CCR5−/− mice that were inoculated with melanoma cells. TUNEL staining revealed a higher frequency of apoptotic cell death in the tumor tissues of the CCR5−/− mice than that of the CCR5+/+ mice (Figure 3A). Immunohistochemical and immunofluorescence showed that high levels of cleaved caspase-3 and Bax were found more frequently in the CCR5−/− mice than in the CCR5+/+ mice (Figure 3B and 3C). Consistent with the immunohistochemical data, Western blot analysis of tumor proteins showed greater increased levels of cleaved caspase-3, cleaved caspase-9, cleaved PARP and Bax, whereas the levels of Bcl-2 and C-IAP1 were significantly decreased in CCR5−/− mice tissues (Figure 3D). 10.1371/journal.pone.0033747.g003 Figure 3 Induction of apoptotic cell death in tumor tissues of CCR5 −/− mice.
T5958 4103-4376 Sentence denotes To see whether inhibition of NF-κB activity results in the induction of apoptotic cell death and expression of NF-κB target apoptotic cell death regulatory protein, the levels of apoptotic cell death and expression of apoptotic cell death regulatory proteins were assessed.
T89 4103-4376 Sentence denotes To see whether inhibition of NF-κB activity results in the induction of apoptotic cell death and expression of NF-κB target apoptotic cell death regulatory protein, the levels of apoptotic cell death and expression of apoptotic cell death regulatory proteins were assessed.
T5959 4377-4508 Sentence denotes Apoptotic cell death in the tumor sections were evaluated in the CCR5+/+ and CCR5−/− mice that were inoculated with melanoma cells.
T90 4377-4508 Sentence denotes Apoptotic cell death in the tumor sections were evaluated in the CCR5+/+ and CCR5−/− mice that were inoculated with melanoma cells.
T5960 4509-4659 Sentence denotes TUNEL staining revealed a higher frequency of apoptotic cell death in the tumor tissues of the CCR5−/− mice than that of the CCR5+/+ mice (Figure 3A).
T91 4509-4659 Sentence denotes TUNEL staining revealed a higher frequency of apoptotic cell death in the tumor tissues of the CCR5−/− mice than that of the CCR5+/+ mice (Figure 3A).
T5961 4660-4847 Sentence denotes Immunohistochemical and immunofluorescence showed that high levels of cleaved caspase-3 and Bax were found more frequently in the CCR5−/− mice than in the CCR5+/+ mice (Figure 3B and 3C).
T92 4660-4847 Sentence denotes Immunohistochemical and immunofluorescence showed that high levels of cleaved caspase-3 and Bax were found more frequently in the CCR5−/− mice than in the CCR5+/+ mice (Figure 3B and 3C).
T5962 4848-5133 Sentence denotes Consistent with the immunohistochemical data, Western blot analysis of tumor proteins showed greater increased levels of cleaved caspase-3, cleaved caspase-9, cleaved PARP and Bax, whereas the levels of Bcl-2 and C-IAP1 were significantly decreased in CCR5−/− mice tissues (Figure 3D).
T93 4848-5133 Sentence denotes Consistent with the immunohistochemical data, Western blot analysis of tumor proteins showed greater increased levels of cleaved caspase-3, cleaved caspase-9, cleaved PARP and Bax, whereas the levels of Bcl-2 and C-IAP1 were significantly decreased in CCR5−/− mice tissues (Figure 3D).
T94 5134-5246 Sentence denotes 10.1371/journal.pone.0033747.g003 Figure 3 Induction of apoptotic cell death in tumor tissues of CCR5 −/− mice.
T18204 5247-5392 Sentence denotes A, Apoptotic cells in tumor sections were examined by fluorescence microscopy after DAPI and TUNEL staining as described in material and methods.
T95 5247-5392 Sentence denotes A, Apoptotic cells in tumor sections were examined by fluorescence microscopy after DAPI and TUNEL staining as described in material and methods.
T18205 5393-5498 Sentence denotes The apoptotic index was determined as the number of DAPI-stained, TUNEL-positive cells that were counted.
T96 5393-5498 Sentence denotes The apoptotic index was determined as the number of DAPI-stained, TUNEL-positive cells that were counted.
T18206 5499-5624 Sentence denotes Values are the mean ± S.D. of four experimental animals. * indicates statistically significant differences from CCR5+/+ mice.
T97 5499-5624 Sentence denotes Values are the mean ± S.D. of four experimental animals. * indicates statistically significant differences from CCR5+/+ mice.
T18207 5625-5651 Sentence denotes Scale bar indicates 50 µm.
T98 5625-5651 Sentence denotes Scale bar indicates 50 µm.
T18208 5652-5767 Sentence denotes B and C, Apoptotic protein (cleaved caspase-3 and Bax) in tumor sections were detected by immunofluorescence assay.
T99 5652-5767 Sentence denotes B and C, Apoptotic protein (cleaved caspase-3 and Bax) in tumor sections were detected by immunofluorescence assay.
T18209 5768-5918 Sentence denotes The reactive cell number was determined as the number of DAPI-stained, Specific antibody (cleaved caspase-3 and Bax)-positive cells that were counted.
T100 5768-5918 Sentence denotes The reactive cell number was determined as the number of DAPI-stained, Specific antibody (cleaved caspase-3 and Bax)-positive cells that were counted.
T18210 5919-6044 Sentence denotes Values are the mean ± S.D. of four experimental animals. * indicates statistically significant differences from CCR5+/+ mice.
T101 5919-6044 Sentence denotes Values are the mean ± S.D. of four experimental animals. * indicates statistically significant differences from CCR5+/+ mice.
T18211 6045-6071 Sentence denotes Scale bar indicates 50 µm.
T102 6045-6071 Sentence denotes Scale bar indicates 50 µm.
T18212 6072-6267 Sentence denotes D, The expression of apoptotic proteins was detected by Western blotting using specific antibodies; cleaved caspase-3, cleaved caspase-9, cleaved PARP, Bax, Bcl-2 and c-IAP1 in the tumor tissues.
T103 6072-6267 Sentence denotes D, The expression of apoptotic proteins was detected by Western blotting using specific antibodies; cleaved caspase-3, cleaved caspase-9, cleaved PARP, Bax, Bcl-2 and c-IAP1 in the tumor tissues.
T18213 6268-6320 Sentence denotes The β-actin protein was used as an internal control.
T104 6268-6320 Sentence denotes The β-actin protein was used as an internal control.
T18214 6321-6391 Sentence denotes Each band is representative of three independent experimental results.
T105 6321-6391 Sentence denotes Each band is representative of three independent experimental results.
T18215 6392-6507 Sentence denotes Data are means ± S.D. of three experimental animals. * indicates significant difference from CCR5+/+ mice (p<0.05).
T106 6392-6507 Sentence denotes Data are means ± S.D. of three experimental animals. * indicates significant difference from CCR5+/+ mice (p<0.05).
T6636 6509-6571 Sentence denotes Upregulation of IL-1Ra in the Tumor and Spleen of CCR5−/− Mice
T107 6509-6571 Sentence denotes Upregulation of IL-1Ra in the Tumor and Spleen of CCR5−/− Mice
T18867 6509-6772 Sentence denotes Upregulation of IL-1Ra in the Tumor and Spleen of CCR5−/− Mice To investigate the difference in the cytokine levels between the tumor and spleen tissues of the CCR5+/+ and CCR5−/− mice, we conducted a cytokine array assay using a Mouse Proteome Array (Figure 4A).
T6637 6572-6772 Sentence denotes To investigate the difference in the cytokine levels between the tumor and spleen tissues of the CCR5+/+ and CCR5−/− mice, we conducted a cytokine array assay using a Mouse Proteome Array (Figure 4A).
T108 6572-6772 Sentence denotes To investigate the difference in the cytokine levels between the tumor and spleen tissues of the CCR5+/+ and CCR5−/− mice, we conducted a cytokine array assay using a Mouse Proteome Array (Figure 4A).
T6638 6773-6924 Sentence denotes Among 40 tested cytokines, the levels of IL-1Ra, MIG, MIP-1α increased about 686, 9 and 25-fold respectively, in CCR5−/− mice compared to CCR5+/+ mice.
T109 6773-6924 Sentence denotes Among 40 tested cytokines, the levels of IL-1Ra, MIG, MIP-1α increased about 686, 9 and 25-fold respectively, in CCR5−/− mice compared to CCR5+/+ mice.
T6639 6925-7051 Sentence denotes Using this technique, no other cytokines were found to be different between the CCR5−/− mice and the CCR5+/+ mice (Figure 4B).
T110 6925-7051 Sentence denotes Using this technique, no other cytokines were found to be different between the CCR5−/− mice and the CCR5+/+ mice (Figure 4B).
T6640 7052-7138 Sentence denotes Similar altered cytokine levels were found in the spleens of CCR5−/− mice (Figure 4C).
T111 7052-7138 Sentence denotes Similar altered cytokine levels were found in the spleens of CCR5−/− mice (Figure 4C).
T6641 7139-7308 Sentence denotes Since the IL-1Ra level was the most significantly elevated, the expression of IL-1Ra in tumor and spleen tissues was analyzed by immunofluorescence and western blotting.
T112 7139-7308 Sentence denotes Since the IL-1Ra level was the most significantly elevated, the expression of IL-1Ra in tumor and spleen tissues was analyzed by immunofluorescence and western blotting.
T6642 7309-7412 Sentence denotes The expression of IL-1Ra was upregulated in tumor and spleen tissues of the CCR5−/− mice (Figure 4D-G).
T113 7309-7412 Sentence denotes The expression of IL-1Ra was upregulated in tumor and spleen tissues of the CCR5−/− mice (Figure 4D-G).
T114 7413-7520 Sentence denotes 10.1371/journal.pone.0033747.g004 Figure 4 Upregulation of IL-1Ra in the tumor and spleen of CCR5−/− mice.
T18868 7521-7583 Sentence denotes A, Figure 4A indicates mouse cytokine array panel coordinates.
T115 7521-7583 Sentence denotes A, Figure 4A indicates mouse cytokine array panel coordinates.
T18869 7584-7676 Sentence denotes Nitrocellulose membranes contain 40 different anti-cytokine antibodies printed in duplicate.
T116 7584-7676 Sentence denotes Nitrocellulose membranes contain 40 different anti-cytokine antibodies printed in duplicate.
T18870 7677-7820 Sentence denotes B, Mouse cytokine array panel indicate the cytokine expression difference in tumor tissues of CCR5+/+ mice and CCR5−/− mice, especially IL-1Ra.
T117 7677-7820 Sentence denotes B, Mouse cytokine array panel indicate the cytokine expression difference in tumor tissues of CCR5+/+ mice and CCR5−/− mice, especially IL-1Ra.
T18871 7821-7885 Sentence denotes Representative blot from three independent experiments is shown.
T118 7821-7885 Sentence denotes Representative blot from three independent experiments is shown.
T18872 7886-7978 Sentence denotes Positive controls show the manufacturer’s internal positive control samples on the membrane.
T119 7886-7978 Sentence denotes Positive controls show the manufacturer’s internal positive control samples on the membrane.
T18873 7979-8064 Sentence denotes C, Protein immune-arrays were performed using Mouse cytokine array in spleen tissues.
T120 7979-8064 Sentence denotes C, Protein immune-arrays were performed using Mouse cytokine array in spleen tissues.
T18874 8065-8158 Sentence denotes There were differences in cytokines between CCR5+/+ mice and CCR5−/− mice, especially IL-1Ra.
T121 8065-8158 Sentence denotes There were differences in cytokines between CCR5+/+ mice and CCR5−/− mice, especially IL-1Ra.
T18875 8159-8223 Sentence denotes Representative blot from three independent experiments is shown.
T122 8159-8223 Sentence denotes Representative blot from three independent experiments is shown.
T18876 8224-8316 Sentence denotes Positive controls show the manufacturer’s internal positive control samples on the membrane.
T123 8224-8316 Sentence denotes Positive controls show the manufacturer’s internal positive control samples on the membrane.
T18877 8317-8437 Sentence denotes D and E, Immunolfluorescence analysis was used to determine the expression levels of IL-1Ra in tumor and spleen tissues.
T124 8317-8437 Sentence denotes D and E, Immunolfluorescence analysis was used to determine the expression levels of IL-1Ra in tumor and spleen tissues.
T18878 8438-8558 Sentence denotes The reactive cell number was determined as the number of DAPI-stained, IL-1Ra antibody-positive cells that were counted.
T125 8438-8558 Sentence denotes The reactive cell number was determined as the number of DAPI-stained, IL-1Ra antibody-positive cells that were counted.
T18879 8559-8684 Sentence denotes Values are the mean ± S.D. of four experimental animals. * indicates statistically significant differences from CCR5+/+ mice.
T126 8559-8684 Sentence denotes Values are the mean ± S.D. of four experimental animals. * indicates statistically significant differences from CCR5+/+ mice.
T18880 8685-8711 Sentence denotes Scale bar indicates 50 µm.
T127 8685-8711 Sentence denotes Scale bar indicates 50 µm.
T18881 8712-8803 Sentence denotes F and G, Expression of IL-1Ra was analyzed by western blotting in tumor and spleen tissues.
T128 8712-8803 Sentence denotes F and G, Expression of IL-1Ra was analyzed by western blotting in tumor and spleen tissues.
T18882 8804-8874 Sentence denotes Each band is representative of three independent experimental results.
T129 8804-8874 Sentence denotes Each band is representative of three independent experimental results.
T18883 8875-8990 Sentence denotes Data are means ± S.D. of three experimental animals. * indicates significant difference from CCR5+/+ mice (p<0.05).
T130 8875-8990 Sentence denotes Data are means ± S.D. of three experimental animals. * indicates significant difference from CCR5+/+ mice (p<0.05).
T7342 8992-9079 Sentence denotes Induction of CD8 T cells and NK Cell Infiltration into Tumor and Spleen of CCR5−/− Mice
T131 8992-9079 Sentence denotes Induction of CD8 T cells and NK Cell Infiltration into Tumor and Spleen of CCR5−/− Mice
T7343 9080-9319 Sentence denotes To investigate whether the inhibition of tumor growth in CCR5−/− mice is related to tumor-specific immune responses, we analyzed the distribution patterns of CD8+ cytotoxic T cell and CD57+ Natural Killer cells in tumor and spleen tissues.
T132 9080-9319 Sentence denotes To investigate whether the inhibition of tumor growth in CCR5−/− mice is related to tumor-specific immune responses, we analyzed the distribution patterns of CD8+ cytotoxic T cell and CD57+ Natural Killer cells in tumor and spleen tissues.
T7344 9320-9519 Sentence denotes By staining the tumor sections from CCR5−/− mice, we found that there was a significant influx of CD8+ cells into the tumor, as well as an increase in the number of infiltrating NK cells (Figure 5A).
T133 9320-9519 Sentence denotes By staining the tumor sections from CCR5−/− mice, we found that there was a significant influx of CD8+ cells into the tumor, as well as an increase in the number of infiltrating NK cells (Figure 5A).
T7345 9520-9605 Sentence denotes CD8+ T cells and CD57+ NK cells were spread diffusely throughout the entire sections.
T134 9520-9605 Sentence denotes CD8+ T cells and CD57+ NK cells were spread diffusely throughout the entire sections.
T7346 9606-9833 Sentence denotes To further investigate the differences in the numbers of CD8+ T cells and CD57+ NK cells between CCR5+/+ mice and CCR5−/− mice in immunity-related organ, we also analyzed the CD8 and CD57 reactive cell number in spleen tissues.
T135 9606-9833 Sentence denotes To further investigate the differences in the numbers of CD8+ T cells and CD57+ NK cells between CCR5+/+ mice and CCR5−/− mice in immunity-related organ, we also analyzed the CD8 and CD57 reactive cell number in spleen tissues.
T7347 9834-9977 Sentence denotes The number of CD8+ T cells and CD57+ NK cells were higher in the spleen of the CCR5−/− mice than in the spleen of the CCR5+/+ mice (Figure 5B).
T136 9834-9977 Sentence denotes The number of CD8+ T cells and CD57+ NK cells were higher in the spleen of the CCR5−/− mice than in the spleen of the CCR5+/+ mice (Figure 5B).
T7348 9978-10168 Sentence denotes These data suggest that by promoting the infiltration of CD8 T cells and NK cells, which are potent cytotoxic effectors for tumors, could play a role in an anti-tumor effect in CCR5−/− mice.
T137 9978-10168 Sentence denotes These data suggest that by promoting the infiltration of CD8 T cells and NK cells, which are potent cytotoxic effectors for tumors, could play a role in an anti-tumor effect in CCR5−/− mice.
T138 10169-10323 Sentence denotes 10.1371/journal.pone.0033747.g005 Figure 5 Induction of the infiltration of CD8+ T cells and NK cells into tumor, and increase in spleen of CCR5−/− mice.
T19587 10213-10323 Sentence denotes Induction of the infiltration of CD8+ T cells and NK cells into tumor, and increase in spleen of CCR5−/− mice.
T19588 10324-10507 Sentence denotes A and B, Immunolfluorescence analysis was used to determine the expression levels of CD8 (CD8+ cytotoxic T-cell surface marker) and CD57 (NK cell marker) in tumor and spleen sections.
T139 10324-10507 Sentence denotes A and B, Immunolfluorescence analysis was used to determine the expression levels of CD8 (CD8+ cytotoxic T-cell surface marker) and CD57 (NK cell marker) in tumor and spleen sections.
T19589 10508-10598 Sentence denotes The images shown are representative of three separate experiments performed in triplicate.
T140 10508-10598 Sentence denotes The images shown are representative of three separate experiments performed in triplicate.
T19590 10599-10644 Sentence denotes Scale bars indicate 50 µm (A) and 100 µm (B).
T141 10599-10644 Sentence denotes Scale bars indicate 50 µm (A) and 100 µm (B).
T19591 10645-10688 Sentence denotes C and D, Analysis of lymphocyte phenotypes.
T142 10645-10688 Sentence denotes C and D, Analysis of lymphocyte phenotypes.
T19592 10689-10763 Sentence denotes The tumor and spleen tissues were separated at study termination (Day 31).
T143 10689-10763 Sentence denotes The tumor and spleen tissues were separated at study termination (Day 31).
T19593 10764-10863 Sentence denotes Flow cytometry analysis was performed using FACSAria flow cytometry, and represent data were shown.
T144 10764-10863 Sentence denotes Flow cytometry analysis was performed using FACSAria flow cytometry, and represent data were shown.
T19594 10864-10915 Sentence denotes Data are means ± S.D. of four experimental animals.
T145 10864-10915 Sentence denotes Data are means ± S.D. of four experimental animals.
T7349 10916-11083 Sentence denotes To analyze the cell phenotype change in CCR5−/− mice and CCR5+/+ mice by inoculation of the melanoma cell, lymphocytes were isolated from the tumor and spleen tissues.
T146 10916-11083 Sentence denotes To analyze the cell phenotype change in CCR5−/− mice and CCR5+/+ mice by inoculation of the melanoma cell, lymphocytes were isolated from the tumor and spleen tissues.
T7350 11084-11289 Sentence denotes The B, CD3 T, CD4 T, CD 8 T and NK cell portion of CCR5−/− mice tumor tissue were 7.2%, 48.2%, 16.9%, 32.1% and 7.8%, respectively, compared to 2.9%, 11.1%, 2.3%, 0.6% and 3.1% of CCR5+/+ mice (Figure 5C).
T147 11084-11289 Sentence denotes The B, CD3 T, CD4 T, CD 8 T and NK cell portion of CCR5−/− mice tumor tissue were 7.2%, 48.2%, 16.9%, 32.1% and 7.8%, respectively, compared to 2.9%, 11.1%, 2.3%, 0.6% and 3.1% of CCR5+/+ mice (Figure 5C).
T7351 11290-11449 Sentence denotes The lymphocytes that infiltrated the tumor tissue of the CCR5−/− mice also had higher levels of CD19, CD3, CD4, CD8 and CD57 when compared to the CCR5+/+ mice.
T148 11290-11449 Sentence denotes The lymphocytes that infiltrated the tumor tissue of the CCR5−/− mice also had higher levels of CD19, CD3, CD4, CD8 and CD57 when compared to the CCR5+/+ mice.
T7352 11450-11656 Sentence denotes The B, CD3 T, CD4 T, CD 8 T and NK cell portion of the CCR5−/− mice spleen were 61.3%, 33.4%, 17.6%, 9.2% and 0.6%, respectively compared to 38.3%, 5.9%, 4.0%, 1.0% and 3.9% of the CCR5+/+ mice (Figure 5D).
T149 11450-11656 Sentence denotes The B, CD3 T, CD4 T, CD 8 T and NK cell portion of the CCR5−/− mice spleen were 61.3%, 33.4%, 17.6%, 9.2% and 0.6%, respectively compared to 38.3%, 5.9%, 4.0%, 1.0% and 3.9% of the CCR5+/+ mice (Figure 5D).
T7353 11657-11857 Sentence denotes These data suggest that by promoting the infiltration of CD8 T cells and NK cells, which are potent cytotoxic effectors for tumors, could play a role in the inhibition of tumor growth in CCR5−/− mice.
T150 11657-11857 Sentence denotes These data suggest that by promoting the infiltration of CD8 T cells and NK cells, which are potent cytotoxic effectors for tumors, could play a role in the inhibition of tumor growth in CCR5−/− mice.