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PMC:3342329 / 11228-13742 JSONTXT 23 Projects

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Id Subject Object Predicate Lexical cue
T5957 0-109 Sentence denotes Induction of Apoptotic Cell Death and Inhibition of the Expression of Anti-apoptotic Proteins in CCR5−/− Mice
T88 0-109 Sentence denotes Induction of Apoptotic Cell Death and Inhibition of the Expression of Anti-apoptotic Proteins in CCR5−/− Mice
T18203 0-1253 Sentence denotes Induction of Apoptotic Cell Death and Inhibition of the Expression of Anti-apoptotic Proteins in CCR5−/− Mice To see whether inhibition of NF-κB activity results in the induction of apoptotic cell death and expression of NF-κB target apoptotic cell death regulatory protein, the levels of apoptotic cell death and expression of apoptotic cell death regulatory proteins were assessed. Apoptotic cell death in the tumor sections were evaluated in the CCR5+/+ and CCR5−/− mice that were inoculated with melanoma cells. TUNEL staining revealed a higher frequency of apoptotic cell death in the tumor tissues of the CCR5−/− mice than that of the CCR5+/+ mice (Figure 3A). Immunohistochemical and immunofluorescence showed that high levels of cleaved caspase-3 and Bax were found more frequently in the CCR5−/− mice than in the CCR5+/+ mice (Figure 3B and 3C). Consistent with the immunohistochemical data, Western blot analysis of tumor proteins showed greater increased levels of cleaved caspase-3, cleaved caspase-9, cleaved PARP and Bax, whereas the levels of Bcl-2 and C-IAP1 were significantly decreased in CCR5−/− mice tissues (Figure 3D). 10.1371/journal.pone.0033747.g003 Figure 3 Induction of apoptotic cell death in tumor tissues of CCR5 −/− mice.
T5958 110-383 Sentence denotes To see whether inhibition of NF-κB activity results in the induction of apoptotic cell death and expression of NF-κB target apoptotic cell death regulatory protein, the levels of apoptotic cell death and expression of apoptotic cell death regulatory proteins were assessed.
T89 110-383 Sentence denotes To see whether inhibition of NF-κB activity results in the induction of apoptotic cell death and expression of NF-κB target apoptotic cell death regulatory protein, the levels of apoptotic cell death and expression of apoptotic cell death regulatory proteins were assessed.
T5959 384-515 Sentence denotes Apoptotic cell death in the tumor sections were evaluated in the CCR5+/+ and CCR5−/− mice that were inoculated with melanoma cells.
T90 384-515 Sentence denotes Apoptotic cell death in the tumor sections were evaluated in the CCR5+/+ and CCR5−/− mice that were inoculated with melanoma cells.
T5960 516-666 Sentence denotes TUNEL staining revealed a higher frequency of apoptotic cell death in the tumor tissues of the CCR5−/− mice than that of the CCR5+/+ mice (Figure 3A).
T91 516-666 Sentence denotes TUNEL staining revealed a higher frequency of apoptotic cell death in the tumor tissues of the CCR5−/− mice than that of the CCR5+/+ mice (Figure 3A).
T5961 667-854 Sentence denotes Immunohistochemical and immunofluorescence showed that high levels of cleaved caspase-3 and Bax were found more frequently in the CCR5−/− mice than in the CCR5+/+ mice (Figure 3B and 3C).
T92 667-854 Sentence denotes Immunohistochemical and immunofluorescence showed that high levels of cleaved caspase-3 and Bax were found more frequently in the CCR5−/− mice than in the CCR5+/+ mice (Figure 3B and 3C).
T5962 855-1140 Sentence denotes Consistent with the immunohistochemical data, Western blot analysis of tumor proteins showed greater increased levels of cleaved caspase-3, cleaved caspase-9, cleaved PARP and Bax, whereas the levels of Bcl-2 and C-IAP1 were significantly decreased in CCR5−/− mice tissues (Figure 3D).
T93 855-1140 Sentence denotes Consistent with the immunohistochemical data, Western blot analysis of tumor proteins showed greater increased levels of cleaved caspase-3, cleaved caspase-9, cleaved PARP and Bax, whereas the levels of Bcl-2 and C-IAP1 were significantly decreased in CCR5−/− mice tissues (Figure 3D).
T94 1141-1253 Sentence denotes 10.1371/journal.pone.0033747.g003 Figure 3 Induction of apoptotic cell death in tumor tissues of CCR5 −/− mice.
T18204 1254-1399 Sentence denotes A, Apoptotic cells in tumor sections were examined by fluorescence microscopy after DAPI and TUNEL staining as described in material and methods.
T95 1254-1399 Sentence denotes A, Apoptotic cells in tumor sections were examined by fluorescence microscopy after DAPI and TUNEL staining as described in material and methods.
T18205 1400-1505 Sentence denotes The apoptotic index was determined as the number of DAPI-stained, TUNEL-positive cells that were counted.
T96 1400-1505 Sentence denotes The apoptotic index was determined as the number of DAPI-stained, TUNEL-positive cells that were counted.
T18206 1506-1631 Sentence denotes Values are the mean ± S.D. of four experimental animals. * indicates statistically significant differences from CCR5+/+ mice.
T97 1506-1631 Sentence denotes Values are the mean ± S.D. of four experimental animals. * indicates statistically significant differences from CCR5+/+ mice.
T18207 1632-1658 Sentence denotes Scale bar indicates 50 µm.
T98 1632-1658 Sentence denotes Scale bar indicates 50 µm.
T18208 1659-1774 Sentence denotes B and C, Apoptotic protein (cleaved caspase-3 and Bax) in tumor sections were detected by immunofluorescence assay.
T99 1659-1774 Sentence denotes B and C, Apoptotic protein (cleaved caspase-3 and Bax) in tumor sections were detected by immunofluorescence assay.
T18209 1775-1925 Sentence denotes The reactive cell number was determined as the number of DAPI-stained, Specific antibody (cleaved caspase-3 and Bax)-positive cells that were counted.
T100 1775-1925 Sentence denotes The reactive cell number was determined as the number of DAPI-stained, Specific antibody (cleaved caspase-3 and Bax)-positive cells that were counted.
T18210 1926-2051 Sentence denotes Values are the mean ± S.D. of four experimental animals. * indicates statistically significant differences from CCR5+/+ mice.
T101 1926-2051 Sentence denotes Values are the mean ± S.D. of four experimental animals. * indicates statistically significant differences from CCR5+/+ mice.
T18211 2052-2078 Sentence denotes Scale bar indicates 50 µm.
T102 2052-2078 Sentence denotes Scale bar indicates 50 µm.
T18212 2079-2274 Sentence denotes D, The expression of apoptotic proteins was detected by Western blotting using specific antibodies; cleaved caspase-3, cleaved caspase-9, cleaved PARP, Bax, Bcl-2 and c-IAP1 in the tumor tissues.
T103 2079-2274 Sentence denotes D, The expression of apoptotic proteins was detected by Western blotting using specific antibodies; cleaved caspase-3, cleaved caspase-9, cleaved PARP, Bax, Bcl-2 and c-IAP1 in the tumor tissues.
T18213 2275-2327 Sentence denotes The β-actin protein was used as an internal control.
T104 2275-2327 Sentence denotes The β-actin protein was used as an internal control.
T18214 2328-2398 Sentence denotes Each band is representative of three independent experimental results.
T105 2328-2398 Sentence denotes Each band is representative of three independent experimental results.
T18215 2399-2514 Sentence denotes Data are means ± S.D. of three experimental animals. * indicates significant difference from CCR5+/+ mice (p<0.05).
T106 2399-2514 Sentence denotes Data are means ± S.D. of three experimental animals. * indicates significant difference from CCR5+/+ mice (p<0.05).