Id |
Subject |
Object |
Predicate |
Lexical cue |
T4054 |
0-57 |
Sentence |
denotes |
Cells of four groups were homogenized as mentioned above. |
T96 |
0-57 |
Sentence |
denotes |
Cells of four groups were homogenized as mentioned above. |
T4055 |
58-180 |
Sentence |
denotes |
The supernatant was collected, and total protein was determined by Coomassie Plus Protein Assay Reagent (Pierce, IL, USA). |
T97 |
58-180 |
Sentence |
denotes |
The supernatant was collected, and total protein was determined by Coomassie Plus Protein Assay Reagent (Pierce, IL, USA). |
T98 |
181-409 |
Sentence |
denotes |
Levels of tumor necrosis factor-alpha (TNF-α), interleukin-1 beta (IL-1β), and interleukin-6 (IL-6) protein were quantified using ELISA kits specific for mouse according to the manufacturer's instructions (Bender MedSystems Inc. |
T4056 |
181-419 |
Sentence |
denotes |
Levels of tumor necrosis factor-alpha (TNF-α), interleukin-1 beta (IL-1β), and interleukin-6 (IL-6) protein were quantified using ELISA kits specific for mouse according to the manufacturer's instructions (Bender MedSystems Inc. CA, USA). |
T99 |
410-419 |
Sentence |
denotes |
CA, USA). |
T4057 |
420-513 |
Sentence |
denotes |
Briefly, prepared the standard and created the standard dilution for building standard curve. |
T100 |
420-513 |
Sentence |
denotes |
Briefly, prepared the standard and created the standard dilution for building standard curve. |
T4058 |
514-578 |
Sentence |
denotes |
Then samples and biotinconjugate were added to microwell strips. |
T101 |
514-578 |
Sentence |
denotes |
Then samples and biotinconjugate were added to microwell strips. |
T4059 |
579-728 |
Sentence |
denotes |
After incubated for 2 h at room temperature, the microwell strips were washed 3 times with wash buffer, and streptavidin-HRP were added to all wells. |
T102 |
579-728 |
Sentence |
denotes |
After incubated for 2 h at room temperature, the microwell strips were washed 3 times with wash buffer, and streptavidin-HRP were added to all wells. |
T4060 |
729-824 |
Sentence |
denotes |
After incubated for 1 h, microwell strips were washed 3 times followed by adding TMB substrate. |
T103 |
729-824 |
Sentence |
denotes |
After incubated for 1 h, microwell strips were washed 3 times followed by adding TMB substrate. |
T4061 |
825-890 |
Sentence |
denotes |
After incubated for about 10–30 min, the stop solution was added. |
T104 |
825-890 |
Sentence |
denotes |
After incubated for about 10–30 min, the stop solution was added. |
T4062 |
891-1007 |
Sentence |
denotes |
The colour intensity was measured at 450 nm using a Bio-Rad ELISA microplate reader (Bio-Rad Laboratories, CA, USA). |
T105 |
891-1007 |
Sentence |
denotes |
The colour intensity was measured at 450 nm using a Bio-Rad ELISA microplate reader (Bio-Rad Laboratories, CA, USA). |
T4063 |
1008-1124 |
Sentence |
denotes |
The concentration of protein was determined according to the standard curve and expressed as pg/mg of total protein. |
T106 |
1008-1124 |
Sentence |
denotes |
The concentration of protein was determined according to the standard curve and expressed as pg/mg of total protein. |