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PMC:3317373 / 9368-10539 JSONTXT 20 Projects

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Id Subject Object Predicate Lexical cue
T4052 0-4 Sentence denotes 2.7.
T94 0-4 Sentence denotes 2.7.
T4053 5-46 Sentence denotes Enzyme-Linked Immunosorbent Assay (ELISA)
T95 5-46 Sentence denotes Enzyme-Linked Immunosorbent Assay (ELISA)
T4054 47-104 Sentence denotes Cells of four groups were homogenized as mentioned above.
T96 47-104 Sentence denotes Cells of four groups were homogenized as mentioned above.
T4055 105-227 Sentence denotes The supernatant was collected, and total protein was determined by Coomassie Plus Protein Assay Reagent (Pierce, IL, USA).
T97 105-227 Sentence denotes The supernatant was collected, and total protein was determined by Coomassie Plus Protein Assay Reagent (Pierce, IL, USA).
T98 228-456 Sentence denotes Levels of tumor necrosis factor-alpha (TNF-α), interleukin-1 beta (IL-1β), and interleukin-6 (IL-6) protein were quantified using ELISA kits specific for mouse according to the manufacturer's instructions (Bender MedSystems Inc.
T4056 228-466 Sentence denotes Levels of tumor necrosis factor-alpha (TNF-α), interleukin-1 beta (IL-1β), and interleukin-6 (IL-6) protein were quantified using ELISA kits specific for mouse according to the manufacturer's instructions (Bender MedSystems Inc. CA, USA).
T99 457-466 Sentence denotes CA, USA).
T4057 467-560 Sentence denotes Briefly, prepared the standard and created the standard dilution for building standard curve.
T100 467-560 Sentence denotes Briefly, prepared the standard and created the standard dilution for building standard curve.
T4058 561-625 Sentence denotes Then samples and biotinconjugate were added to microwell strips.
T101 561-625 Sentence denotes Then samples and biotinconjugate were added to microwell strips.
T4059 626-775 Sentence denotes After incubated for 2 h at room temperature, the microwell strips were washed 3 times with wash buffer, and streptavidin-HRP were added to all wells.
T102 626-775 Sentence denotes After incubated for 2 h at room temperature, the microwell strips were washed 3 times with wash buffer, and streptavidin-HRP were added to all wells.
T4060 776-871 Sentence denotes After incubated for 1 h, microwell strips were washed 3 times followed by adding TMB substrate.
T103 776-871 Sentence denotes After incubated for 1 h, microwell strips were washed 3 times followed by adding TMB substrate.
T4061 872-937 Sentence denotes After incubated for about 10–30 min, the stop solution was added.
T104 872-937 Sentence denotes After incubated for about 10–30 min, the stop solution was added.
T4062 938-1054 Sentence denotes The colour intensity was measured at 450 nm using a Bio-Rad ELISA microplate reader (Bio-Rad Laboratories, CA, USA).
T105 938-1054 Sentence denotes The colour intensity was measured at 450 nm using a Bio-Rad ELISA microplate reader (Bio-Rad Laboratories, CA, USA).
T4063 1055-1171 Sentence denotes The concentration of protein was determined according to the standard curve and expressed as pg/mg of total protein.
T106 1055-1171 Sentence denotes The concentration of protein was determined according to the standard curve and expressed as pg/mg of total protein.