| Id |
Subject |
Object |
Predicate |
Lexical cue |
| T85 |
0-279 |
Sentence |
denotes |
To obtain total protein lysates, cells were homogenized in RIPA buffer (1% NP40, 0.5% sodium deoxycholate, 0.1% SDS, 1 mM EDTA, 1 mM EGTA, 1 mM Na3VO4, 20 mM NaF, 0.5 mM DTT, 1 mM PMSF, and protease inhibitor cocktail in PBS pH 7.4) and centrifuged at 12,000 g for 15 min at 4°C. |
| T3376 |
0-376 |
Sentence |
denotes |
To obtain total protein lysates, cells were homogenized in RIPA buffer (1% NP40, 0.5% sodium deoxycholate, 0.1% SDS, 1 mM EDTA, 1 mM EGTA, 1 mM Na3VO4, 20 mM NaF, 0.5 mM DTT, 1 mM PMSF, and protease inhibitor cocktail in PBS pH 7.4) and centrifuged at 12,000 g for 15 min at 4°C. Protein concentrations were estimated by Coomassie Plus Protein Assay Reagent (Pierce, IL, USA). |
| T86 |
280-376 |
Sentence |
denotes |
Protein concentrations were estimated by Coomassie Plus Protein Assay Reagent (Pierce, IL, USA). |
| T3377 |
377-618 |
Sentence |
denotes |
Fifty micrograms of the resulting cytosolic protein extracts were heat-denatured in Laemmli sample loading buffer, separated by 10% sodium dodecyl sulfate polyacrylamide gel electrophoresis, and electroblotted onto a nitrocellulose membrane. |
| T87 |
377-618 |
Sentence |
denotes |
Fifty micrograms of the resulting cytosolic protein extracts were heat-denatured in Laemmli sample loading buffer, separated by 10% sodium dodecyl sulfate polyacrylamide gel electrophoresis, and electroblotted onto a nitrocellulose membrane. |
| T3378 |
619-924 |
Sentence |
denotes |
For immunoblotting, membranes were blocked with 5% nonfat dry milk in saline buffer overnight at 4°C, and the following antibodies were used: anti-β-actin (sc-130657, Santa Cruz Biotechnology, CA, USA, 43 kDa) and anti-matrix metallopeptidase 9 (MMP9) (sc-6841, Santa Cruz Biotechnology, CA, USA, 92 kDa). |
| T88 |
619-924 |
Sentence |
denotes |
For immunoblotting, membranes were blocked with 5% nonfat dry milk in saline buffer overnight at 4°C, and the following antibodies were used: anti-β-actin (sc-130657, Santa Cruz Biotechnology, CA, USA, 43 kDa) and anti-matrix metallopeptidase 9 (MMP9) (sc-6841, Santa Cruz Biotechnology, CA, USA, 92 kDa). |
| T3379 |
925-1048 |
Sentence |
denotes |
Each primary antibody was diluted appropriately in blocking buffer and then added to the blots for 1 h at room temperature. |
| T89 |
925-1048 |
Sentence |
denotes |
Each primary antibody was diluted appropriately in blocking buffer and then added to the blots for 1 h at room temperature. |
| T3380 |
1049-1206 |
Sentence |
denotes |
The blots were washed three times in the washing buffer and covered with the horseradish peroxidase-linked secondary antibody at a 1 : 2000 dilution for 1 h. |
| T90 |
1049-1206 |
Sentence |
denotes |
The blots were washed three times in the washing buffer and covered with the horseradish peroxidase-linked secondary antibody at a 1 : 2000 dilution for 1 h. |
| T3381 |
1207-1381 |
Sentence |
denotes |
Blots were incubated with enhanced chemiluminescence (ECL) detection system (Amersham Biosciences, Bucks, UK) and exposed to radiographic film (Fuji Hyperfilm, Tokyo, Japan). |
| T91 |
1207-1381 |
Sentence |
denotes |
Blots were incubated with enhanced chemiluminescence (ECL) detection system (Amersham Biosciences, Bucks, UK) and exposed to radiographic film (Fuji Hyperfilm, Tokyo, Japan). |
| T3382 |
1382-1436 |
Sentence |
denotes |
ImageJ was used to analyze the intensity of the blots. |
| T92 |
1382-1436 |
Sentence |
denotes |
ImageJ was used to analyze the intensity of the blots. |
| T3383 |
1437-1488 |
Sentence |
denotes |
The level of β-actin was used as internal standard. |
| T93 |
1437-1488 |
Sentence |
denotes |
The level of β-actin was used as internal standard. |
