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PMC:3317373 / 7259-7858 JSONTXT 21 Projects

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Id Subject Object Predicate Lexical cue
T3100 0-4 Sentence denotes 2.5.
T74 0-4 Sentence denotes 2.5.
T3101 5-11 Sentence denotes RT-PCR
T75 5-11 Sentence denotes RT-PCR
T3102 12-224 Sentence denotes Total RNA was isolated with Trizol (Invitrogen, CA, USA), and single-stranded cDNA was synthesized from 2 μg of total RNA with BU-Script RT-Kit (Biunique, Jiangsu, China) according to the manufacturer's protocol.
T76 12-224 Sentence denotes Total RNA was isolated with Trizol (Invitrogen, CA, USA), and single-stranded cDNA was synthesized from 2 μg of total RNA with BU-Script RT-Kit (Biunique, Jiangsu, China) according to the manufacturer's protocol.
T77 225-254 Sentence denotes The cDNA was stored in −20°C.
T3103 225-379 Sentence denotes The cDNA was stored in −20°C. Reverse transcription was conducted with GoTaq Green Master Mix (Promega, WI, USA) according to the manufacturer's protocol.
T78 255-379 Sentence denotes Reverse transcription was conducted with GoTaq Green Master Mix (Promega, WI, USA) according to the manufacturer's protocol.
T3104 381-426 Sentence denotes Table 1 shows the primers and PCR parameters.
T79 381-426 Sentence denotes Table 1 shows the primers and PCR parameters.
T3105 427-485 Sentence denotes PCR products were detected by agarose gel electrophoresis.
T80 427-485 Sentence denotes PCR products were detected by agarose gel electrophoresis.
T3106 486-544 Sentence denotes The intensity of the bands was analyzed by ImageJ program.
T81 486-544 Sentence denotes The intensity of the bands was analyzed by ImageJ program.
T3107 545-599 Sentence denotes The level of β-actin was used as an internal standard.
T82 545-599 Sentence denotes The level of β-actin was used as an internal standard.