PMC:3317373 / 4884-5454 JSONTXT 10 Projects

Annnotations TAB TSV DIC JSON TextAE

Id Subject Object Predicate Lexical cue
T2035 0-91 Sentence denotes 1 × 103/well cells were seeded to 96-well culture plates and cultivated for 24 h to adhere.
T52 0-91 Sentence denotes 1 × 103/well cells were seeded to 96-well culture plates and cultivated for 24 h to adhere.
T2036 92-137 Sentence denotes Then the cell was scrated as mentioned above.
T53 92-137 Sentence denotes Then the cell was scrated as mentioned above.
T2037 138-286 Sentence denotes Astrocyte cell death was assessed by cell counting kit-8 (CCK-8) (Dojindo, Japan) assay 24 h after scratch according to the manufacturer's protocol.
T54 138-286 Sentence denotes Astrocyte cell death was assessed by cell counting kit-8 (CCK-8) (Dojindo, Japan) assay 24 h after scratch according to the manufacturer's protocol.
T2038 287-356 Sentence denotes Briefly, 10 μL CCK-8 was added into every well and incubated for 1 h.
T55 287-356 Sentence denotes Briefly, 10 μL CCK-8 was added into every well and incubated for 1 h.
T2039 357-462 Sentence denotes Then OD value was read at 450 nm using a Bio-Rad ELISA microplate reader (Bio-Rad Laboratories, CA, USA).
T56 357-462 Sentence denotes Then OD value was read at 450 nm using a Bio-Rad ELISA microplate reader (Bio-Rad Laboratories, CA, USA).
T2040 463-511 Sentence denotes All measurements were performed in sextuplicate.
T57 463-511 Sentence denotes All measurements were performed in sextuplicate.
T2041 512-570 Sentence denotes Results were expressed as mean of OD value at 450 nm ± SD.
T58 512-570 Sentence denotes Results were expressed as mean of OD value at 450 nm ± SD.