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PMC:3317373 / 4841-5454 JSONTXT 10 Projects

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Id Subject Object Predicate Lexical cue
T2033 0-4 Sentence denotes 2.3.
T50 0-4 Sentence denotes 2.3.
T2034 5-42 Sentence denotes Cell Death Measurement by CCK-8 Assay
T51 5-42 Sentence denotes Cell Death Measurement by CCK-8 Assay
T2035 43-134 Sentence denotes 1 × 103/well cells were seeded to 96-well culture plates and cultivated for 24 h to adhere.
T52 43-134 Sentence denotes 1 × 103/well cells were seeded to 96-well culture plates and cultivated for 24 h to adhere.
T2036 135-180 Sentence denotes Then the cell was scrated as mentioned above.
T53 135-180 Sentence denotes Then the cell was scrated as mentioned above.
T2037 181-329 Sentence denotes Astrocyte cell death was assessed by cell counting kit-8 (CCK-8) (Dojindo, Japan) assay 24 h after scratch according to the manufacturer's protocol.
T54 181-329 Sentence denotes Astrocyte cell death was assessed by cell counting kit-8 (CCK-8) (Dojindo, Japan) assay 24 h after scratch according to the manufacturer's protocol.
T2038 330-399 Sentence denotes Briefly, 10 μL CCK-8 was added into every well and incubated for 1 h.
T55 330-399 Sentence denotes Briefly, 10 μL CCK-8 was added into every well and incubated for 1 h.
T2039 400-505 Sentence denotes Then OD value was read at 450 nm using a Bio-Rad ELISA microplate reader (Bio-Rad Laboratories, CA, USA).
T56 400-505 Sentence denotes Then OD value was read at 450 nm using a Bio-Rad ELISA microplate reader (Bio-Rad Laboratories, CA, USA).
T2040 506-554 Sentence denotes All measurements were performed in sextuplicate.
T57 506-554 Sentence denotes All measurements were performed in sextuplicate.
T2041 555-613 Sentence denotes Results were expressed as mean of OD value at 450 nm ± SD.
T58 555-613 Sentence denotes Results were expressed as mean of OD value at 450 nm ± SD.