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PMC:3317373 / 2879-4307 JSONTXT 10 Projects

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Id Subject Object Predicate Lexical cue
T1224 0-4 Sentence denotes 2.1.
T28 0-4 Sentence denotes 2.1.
T1225 5-59 Sentence denotes Primary Culture and Identification of Mouse Astrocytes
T29 5-59 Sentence denotes Primary Culture and Identification of Mouse Astrocytes
T30 60-120 Sentence denotes Nrf2 knockout ICR mice were kindly provided by Dr. Thomas W.
T1226 60-176 Sentence denotes Nrf2 knockout ICR mice were kindly provided by Dr. Thomas W. Kensler (Johns Hopkins University, Baltimore, MD, USA).
T31 121-176 Sentence denotes Kensler (Johns Hopkins University, Baltimore, MD, USA).
T1227 177-305 Sentence denotes Primary astrocytes were obtained from postnatal 2-day-old Nrf2 wide type (WT) and knockout (KO) mice (6 mice for each genotype).
T32 177-305 Sentence denotes Primary astrocytes were obtained from postnatal 2-day-old Nrf2 wide type (WT) and knockout (KO) mice (6 mice for each genotype).
T1228 306-422 Sentence denotes Following decapitation, the cortices were dissected out, and the meninges and associated blood vessels were removed.
T33 306-422 Sentence denotes Following decapitation, the cortices were dissected out, and the meninges and associated blood vessels were removed.
T1229 423-557 Sentence denotes The tissue was roughly chopped with a scalpel blade, incubated in 0.5% trypsin for 10 minutes at 37°C, and agitated every few minutes.
T34 423-557 Sentence denotes The tissue was roughly chopped with a scalpel blade, incubated in 0.5% trypsin for 10 minutes at 37°C, and agitated every few minutes.
T1230 558-758 Sentence denotes After digestion, the tissue was rinsed twice in DMEM with 10% FBS, followed by a mechanical dissociation in DMEM with 20% FBS and 5 units/mL penicillin, 5 μg/mL streptomycin (complete culture medium).
T35 558-758 Sentence denotes After digestion, the tissue was rinsed twice in DMEM with 10% FBS, followed by a mechanical dissociation in DMEM with 20% FBS and 5 units/mL penicillin, 5 μg/mL streptomycin (complete culture medium).
T1231 759-898 Sentence denotes After incubation for 1 h, the supernatant was transferred to a new flask or dish (Costar, USA) for depleting the residual epithelial cells.
T36 759-898 Sentence denotes After incubation for 1 h, the supernatant was transferred to a new flask or dish (Costar, USA) for depleting the residual epithelial cells.
T1232 899-950 Sentence denotes Then the cells were cultivated at 37°C with 5% CO2.
T37 899-950 Sentence denotes Then the cells were cultivated at 37°C with 5% CO2.
T1233 951-1013 Sentence denotes And the complete culture medium was half-changed twice a week.
T38 951-1013 Sentence denotes And the complete culture medium was half-changed twice a week.
T1234 1014-1071 Sentence denotes Astrocytes expanded for about 7 days to reach confluence.
T39 1014-1071 Sentence denotes Astrocytes expanded for about 7 days to reach confluence.
T1235 1072-1196 Sentence denotes Then the flasks or dishes were shaken at 150 rpm for 4 h to deplete the microglia and less adherent cells from the cultures.
T40 1072-1196 Sentence denotes Then the flasks or dishes were shaken at 150 rpm for 4 h to deplete the microglia and less adherent cells from the cultures.
T1236 1197-1357 Sentence denotes After shaking, the resulting cultures were mainly astrocytes, which were determined by immunoreactivity for GFAP (sc-166481, Santa Cruz Biotechnology, CA, USA).
T41 1197-1357 Sentence denotes After shaking, the resulting cultures were mainly astrocytes, which were determined by immunoreactivity for GFAP (sc-166481, Santa Cruz Biotechnology, CA, USA).
T1237 1358-1428 Sentence denotes Cells passaged for 2-3 generations were used in the following studies.
T42 1358-1428 Sentence denotes Cells passaged for 2-3 generations were used in the following studies.