sentences Home  

PMC:3317373 / 2853-11658 JSONTXT 25 Projects

Annnotations TAB TSV DIC JSON TextAE

Id Subject Object Predicate Lexical cue
T26 0-2 Sentence denotes 2.
T27 3-24 Sentence denotes Methods and Materials
T1224 26-30 Sentence denotes 2.1.
T28 26-30 Sentence denotes 2.1.
T1225 31-85 Sentence denotes Primary Culture and Identification of Mouse Astrocytes
T29 31-85 Sentence denotes Primary Culture and Identification of Mouse Astrocytes
T30 86-146 Sentence denotes Nrf2 knockout ICR mice were kindly provided by Dr. Thomas W.
T1226 86-202 Sentence denotes Nrf2 knockout ICR mice were kindly provided by Dr. Thomas W. Kensler (Johns Hopkins University, Baltimore, MD, USA).
T31 147-202 Sentence denotes Kensler (Johns Hopkins University, Baltimore, MD, USA).
T1227 203-331 Sentence denotes Primary astrocytes were obtained from postnatal 2-day-old Nrf2 wide type (WT) and knockout (KO) mice (6 mice for each genotype).
T32 203-331 Sentence denotes Primary astrocytes were obtained from postnatal 2-day-old Nrf2 wide type (WT) and knockout (KO) mice (6 mice for each genotype).
T1228 332-448 Sentence denotes Following decapitation, the cortices were dissected out, and the meninges and associated blood vessels were removed.
T33 332-448 Sentence denotes Following decapitation, the cortices were dissected out, and the meninges and associated blood vessels were removed.
T1229 449-583 Sentence denotes The tissue was roughly chopped with a scalpel blade, incubated in 0.5% trypsin for 10 minutes at 37°C, and agitated every few minutes.
T34 449-583 Sentence denotes The tissue was roughly chopped with a scalpel blade, incubated in 0.5% trypsin for 10 minutes at 37°C, and agitated every few minutes.
T1230 584-784 Sentence denotes After digestion, the tissue was rinsed twice in DMEM with 10% FBS, followed by a mechanical dissociation in DMEM with 20% FBS and 5 units/mL penicillin, 5 μg/mL streptomycin (complete culture medium).
T35 584-784 Sentence denotes After digestion, the tissue was rinsed twice in DMEM with 10% FBS, followed by a mechanical dissociation in DMEM with 20% FBS and 5 units/mL penicillin, 5 μg/mL streptomycin (complete culture medium).
T1231 785-924 Sentence denotes After incubation for 1 h, the supernatant was transferred to a new flask or dish (Costar, USA) for depleting the residual epithelial cells.
T36 785-924 Sentence denotes After incubation for 1 h, the supernatant was transferred to a new flask or dish (Costar, USA) for depleting the residual epithelial cells.
T1232 925-976 Sentence denotes Then the cells were cultivated at 37°C with 5% CO2.
T37 925-976 Sentence denotes Then the cells were cultivated at 37°C with 5% CO2.
T1233 977-1039 Sentence denotes And the complete culture medium was half-changed twice a week.
T38 977-1039 Sentence denotes And the complete culture medium was half-changed twice a week.
T1234 1040-1097 Sentence denotes Astrocytes expanded for about 7 days to reach confluence.
T39 1040-1097 Sentence denotes Astrocytes expanded for about 7 days to reach confluence.
T1235 1098-1222 Sentence denotes Then the flasks or dishes were shaken at 150 rpm for 4 h to deplete the microglia and less adherent cells from the cultures.
T40 1098-1222 Sentence denotes Then the flasks or dishes were shaken at 150 rpm for 4 h to deplete the microglia and less adherent cells from the cultures.
T1236 1223-1383 Sentence denotes After shaking, the resulting cultures were mainly astrocytes, which were determined by immunoreactivity for GFAP (sc-166481, Santa Cruz Biotechnology, CA, USA).
T41 1223-1383 Sentence denotes After shaking, the resulting cultures were mainly astrocytes, which were determined by immunoreactivity for GFAP (sc-166481, Santa Cruz Biotechnology, CA, USA).
T1237 1384-1454 Sentence denotes Cells passaged for 2-3 generations were used in the following studies.
T42 1384-1454 Sentence denotes Cells passaged for 2-3 generations were used in the following studies.
T1813 1456-1460 Sentence denotes 2.2.
T43 1456-1460 Sentence denotes 2.2.
T1814 1461-1512 Sentence denotes In Vitro Model of TBI Established by Scratch Injury
T44 1461-1512 Sentence denotes In Vitro Model of TBI Established by Scratch Injury
T1815 1513-1580 Sentence denotes Astrocyte scratch injury was performed as in a previous report [7].
T45 1513-1580 Sentence denotes Astrocyte scratch injury was performed as in a previous report [7].
T1816 1581-1646 Sentence denotes Astrocytes were planted in 6-well plates and grown to confluence.
T46 1581-1646 Sentence denotes Astrocytes were planted in 6-well plates and grown to confluence.
T1817 1647-1763 Sentence denotes The cell monolayer was scratched with a sterile 26G syringe needle, resulting in the formation of a 0.5 mm wide gap.
T47 1647-1763 Sentence denotes The cell monolayer was scratched with a sterile 26G syringe needle, resulting in the formation of a 0.5 mm wide gap.
T1818 1764-1895 Sentence denotes Immediately after scratch, cells were washed twice with sterile PBS, cultured with complete culture medium, and named as TBI group.
T48 1764-1895 Sentence denotes Immediately after scratch, cells were washed twice with sterile PBS, cultured with complete culture medium, and named as TBI group.
T1819 1896-1986 Sentence denotes Cells, which did not received scratch, were used as blank control and named as sham group.
T49 1896-1986 Sentence denotes Cells, which did not received scratch, were used as blank control and named as sham group.
T2033 1988-1992 Sentence denotes 2.3.
T50 1988-1992 Sentence denotes 2.3.
T2034 1993-2030 Sentence denotes Cell Death Measurement by CCK-8 Assay
T51 1993-2030 Sentence denotes Cell Death Measurement by CCK-8 Assay
T2035 2031-2122 Sentence denotes 1 × 103/well cells were seeded to 96-well culture plates and cultivated for 24 h to adhere.
T52 2031-2122 Sentence denotes 1 × 103/well cells were seeded to 96-well culture plates and cultivated for 24 h to adhere.
T2036 2123-2168 Sentence denotes Then the cell was scrated as mentioned above.
T53 2123-2168 Sentence denotes Then the cell was scrated as mentioned above.
T2037 2169-2317 Sentence denotes Astrocyte cell death was assessed by cell counting kit-8 (CCK-8) (Dojindo, Japan) assay 24 h after scratch according to the manufacturer's protocol.
T54 2169-2317 Sentence denotes Astrocyte cell death was assessed by cell counting kit-8 (CCK-8) (Dojindo, Japan) assay 24 h after scratch according to the manufacturer's protocol.
T2038 2318-2387 Sentence denotes Briefly, 10 μL CCK-8 was added into every well and incubated for 1 h.
T55 2318-2387 Sentence denotes Briefly, 10 μL CCK-8 was added into every well and incubated for 1 h.
T2039 2388-2493 Sentence denotes Then OD value was read at 450 nm using a Bio-Rad ELISA microplate reader (Bio-Rad Laboratories, CA, USA).
T56 2388-2493 Sentence denotes Then OD value was read at 450 nm using a Bio-Rad ELISA microplate reader (Bio-Rad Laboratories, CA, USA).
T2040 2494-2542 Sentence denotes All measurements were performed in sextuplicate.
T57 2494-2542 Sentence denotes All measurements were performed in sextuplicate.
T2041 2543-2601 Sentence denotes Results were expressed as mean of OD value at 450 nm ± SD.
T58 2543-2601 Sentence denotes Results were expressed as mean of OD value at 450 nm ± SD.
T2309 2603-2607 Sentence denotes 2.4.
T59 2603-2607 Sentence denotes 2.4.
T2310 2608-2651 Sentence denotes Electrophoretic Mobility Shift Assay (EMSA)
T60 2608-2651 Sentence denotes Electrophoretic Mobility Shift Assay (EMSA)
T2311 2652-2738 Sentence denotes Monolayers of astrocytes were washed with PBS and harvested by scraping into cold PBS.
T61 2652-2738 Sentence denotes Monolayers of astrocytes were washed with PBS and harvested by scraping into cold PBS.
T2312 2739-2931 Sentence denotes The cell pellet obtained by centrifugation was resuspended in buffer containing 10 mM HEPES (pH 7.9), 10 mM KCl, 0.1 mM EDTA, 0.1 mM EGTA, 1.0 mM DTT, and 0.5 mM phenylmethylsulfonyl fluoride.
T62 2739-2931 Sentence denotes The cell pellet obtained by centrifugation was resuspended in buffer containing 10 mM HEPES (pH 7.9), 10 mM KCl, 0.1 mM EDTA, 0.1 mM EGTA, 1.0 mM DTT, and 0.5 mM phenylmethylsulfonyl fluoride.
T2313 2932-3033 Sentence denotes Then 10% Nonidet P-40 was added and vortexed briefly, and the nuclei were pelleted by centrifugation.
T63 2932-3033 Sentence denotes Then 10% Nonidet P-40 was added and vortexed briefly, and the nuclei were pelleted by centrifugation.
T2314 3034-3207 Sentence denotes The nuclear proteins were extracted with buffer containing 20 mM HEPES (pH 7.9), 0.4 mM NaCl, 1.0 mM EDTA, 1.0 mM EGTA, 1.0 mM DTT, and 1.0 mM phenylmethylsulfonyl fluoride.
T64 3034-3207 Sentence denotes The nuclear proteins were extracted with buffer containing 20 mM HEPES (pH 7.9), 0.4 mM NaCl, 1.0 mM EDTA, 1.0 mM EGTA, 1.0 mM DTT, and 1.0 mM phenylmethylsulfonyl fluoride.
T2315 3208-3353 Sentence denotes Insoluble material was removed by centrifugation at 14000 rpm, and the supernatant containing the nuclear proteins was stored at –80°C until use.
T65 3208-3353 Sentence denotes Insoluble material was removed by centrifugation at 14000 rpm, and the supernatant containing the nuclear proteins was stored at –80°C until use.
T2316 3354-3511 Sentence denotes Protein concentration was determined using a bicinchoninic acid assay kit with bovine serum albumin as the standard (Pierce Biochemicals, Rockford, IL, USA).
T66 3354-3511 Sentence denotes Protein concentration was determined using a bicinchoninic acid assay kit with bovine serum albumin as the standard (Pierce Biochemicals, Rockford, IL, USA).
T2317 3512-3588 Sentence denotes EMSA was performed using gel shift assay system (Promega, Madison, WI, USA).
T67 3512-3588 Sentence denotes EMSA was performed using gel shift assay system (Promega, Madison, WI, USA).
T2318 3589-3746 Sentence denotes Consensus oligonucleotide probe (5′-AGTTGAGGGGACTTTCCCAGGG-3′) was end-labeled by T4-polynucleotide kinase using [γ-32P]-ATP (Free Biotech., Beijing, China).
T68 3589-3746 Sentence denotes Consensus oligonucleotide probe (5′-AGTTGAGGGGACTTTCCCAGGG-3′) was end-labeled by T4-polynucleotide kinase using [γ-32P]-ATP (Free Biotech., Beijing, China).
T2319 3747-3953 Sentence denotes Nuclear protein (20 μg) was preincubated in 20 μL binding buffer containing 10 mM Tris-HCl (PH 7.5), 1 mM MgCl2, 0.5 mM NaCl, 4% glycerol, 0.5 mM EDTA, 0.5 mM DTT, and 2 μg poly dI-dC for 20 minutes on ice.
T69 3747-3953 Sentence denotes Nuclear protein (20 μg) was preincubated in 20 μL binding buffer containing 10 mM Tris-HCl (PH 7.5), 1 mM MgCl2, 0.5 mM NaCl, 4% glycerol, 0.5 mM EDTA, 0.5 mM DTT, and 2 μg poly dI-dC for 20 minutes on ice.
T2320 3954-4066 Sentence denotes After addition of the 1 μL 32P-labled oligonucleotide probe, the incubation was continued for 20 minutes on ice.
T70 3954-4066 Sentence denotes After addition of the 1 μL 32P-labled oligonucleotide probe, the incubation was continued for 20 minutes on ice.
T71 4067-4232 Sentence denotes The DNA-protein complexes were separated by electrophoresis on 4% nondenaturing polyacrylamide gel in 0.5 × TBE buffer (tris-borate-EDTA) at 390 V for 1 hour at 4°C.
T2321 4067-4331 Sentence denotes The DNA-protein complexes were separated by electrophoresis on 4% nondenaturing polyacrylamide gel in 0.5 × TBE buffer (tris-borate-EDTA) at 390 V for 1 hour at 4°C. After electrophoresis, the gel was dried and exposed to X-ray film (Fuji Hyperfilm, Tokyo, Japan).
T72 4233-4331 Sentence denotes After electrophoresis, the gel was dried and exposed to X-ray film (Fuji Hyperfilm, Tokyo, Japan).
T2322 4332-4404 Sentence denotes Levels of NF-κB DNA binding activity were quantified by software ImageJ.
T73 4332-4404 Sentence denotes Levels of NF-κB DNA binding activity were quantified by software ImageJ.
T3100 4406-4410 Sentence denotes 2.5.
T74 4406-4410 Sentence denotes 2.5.
T3101 4411-4417 Sentence denotes RT-PCR
T75 4411-4417 Sentence denotes RT-PCR
T3102 4418-4630 Sentence denotes Total RNA was isolated with Trizol (Invitrogen, CA, USA), and single-stranded cDNA was synthesized from 2 μg of total RNA with BU-Script RT-Kit (Biunique, Jiangsu, China) according to the manufacturer's protocol.
T76 4418-4630 Sentence denotes Total RNA was isolated with Trizol (Invitrogen, CA, USA), and single-stranded cDNA was synthesized from 2 μg of total RNA with BU-Script RT-Kit (Biunique, Jiangsu, China) according to the manufacturer's protocol.
T77 4631-4660 Sentence denotes The cDNA was stored in −20°C.
T3103 4631-4785 Sentence denotes The cDNA was stored in −20°C. Reverse transcription was conducted with GoTaq Green Master Mix (Promega, WI, USA) according to the manufacturer's protocol.
T78 4661-4785 Sentence denotes Reverse transcription was conducted with GoTaq Green Master Mix (Promega, WI, USA) according to the manufacturer's protocol.
T3104 4787-4832 Sentence denotes Table 1 shows the primers and PCR parameters.
T79 4787-4832 Sentence denotes Table 1 shows the primers and PCR parameters.
T3105 4833-4891 Sentence denotes PCR products were detected by agarose gel electrophoresis.
T80 4833-4891 Sentence denotes PCR products were detected by agarose gel electrophoresis.
T3106 4892-4950 Sentence denotes The intensity of the bands was analyzed by ImageJ program.
T81 4892-4950 Sentence denotes The intensity of the bands was analyzed by ImageJ program.
T3107 4951-5005 Sentence denotes The level of β-actin was used as an internal standard.
T82 4951-5005 Sentence denotes The level of β-actin was used as an internal standard.
T3374 5007-5011 Sentence denotes 2.6.
T83 5007-5011 Sentence denotes 2.6.
T3375 5012-5024 Sentence denotes Western Blot
T84 5012-5024 Sentence denotes Western Blot
T85 5025-5304 Sentence denotes To obtain total protein lysates, cells were homogenized in RIPA buffer (1% NP40, 0.5% sodium deoxycholate, 0.1% SDS, 1 mM EDTA, 1 mM EGTA, 1 mM Na3VO4, 20 mM NaF, 0.5 mM DTT, 1 mM PMSF, and protease inhibitor cocktail in PBS pH 7.4) and centrifuged at 12,000 g for 15 min at 4°C.
T3376 5025-5401 Sentence denotes To obtain total protein lysates, cells were homogenized in RIPA buffer (1% NP40, 0.5% sodium deoxycholate, 0.1% SDS, 1 mM EDTA, 1 mM EGTA, 1 mM Na3VO4, 20 mM NaF, 0.5 mM DTT, 1 mM PMSF, and protease inhibitor cocktail in PBS pH 7.4) and centrifuged at 12,000 g for 15 min at 4°C. Protein concentrations were estimated by Coomassie Plus Protein Assay Reagent (Pierce, IL, USA).
T86 5305-5401 Sentence denotes Protein concentrations were estimated by Coomassie Plus Protein Assay Reagent (Pierce, IL, USA).
T3377 5402-5643 Sentence denotes Fifty micrograms of the resulting cytosolic protein extracts were heat-denatured in Laemmli sample loading buffer, separated by 10% sodium dodecyl sulfate polyacrylamide gel electrophoresis, and electroblotted onto a nitrocellulose membrane.
T87 5402-5643 Sentence denotes Fifty micrograms of the resulting cytosolic protein extracts were heat-denatured in Laemmli sample loading buffer, separated by 10% sodium dodecyl sulfate polyacrylamide gel electrophoresis, and electroblotted onto a nitrocellulose membrane.
T3378 5644-5949 Sentence denotes For immunoblotting, membranes were blocked with 5% nonfat dry milk in saline buffer overnight at 4°C, and the following antibodies were used: anti-β-actin (sc-130657, Santa Cruz Biotechnology, CA, USA, 43 kDa) and anti-matrix metallopeptidase 9 (MMP9) (sc-6841, Santa Cruz Biotechnology, CA, USA, 92 kDa).
T88 5644-5949 Sentence denotes For immunoblotting, membranes were blocked with 5% nonfat dry milk in saline buffer overnight at 4°C, and the following antibodies were used: anti-β-actin (sc-130657, Santa Cruz Biotechnology, CA, USA, 43 kDa) and anti-matrix metallopeptidase 9 (MMP9) (sc-6841, Santa Cruz Biotechnology, CA, USA, 92 kDa).
T3379 5950-6073 Sentence denotes Each primary antibody was diluted appropriately in blocking buffer and then added to the blots for 1 h at room temperature.
T89 5950-6073 Sentence denotes Each primary antibody was diluted appropriately in blocking buffer and then added to the blots for 1 h at room temperature.
T3380 6074-6231 Sentence denotes The blots were washed three times in the washing buffer and covered with the horseradish peroxidase-linked secondary antibody at a 1 : 2000 dilution for 1 h.
T90 6074-6231 Sentence denotes The blots were washed three times in the washing buffer and covered with the horseradish peroxidase-linked secondary antibody at a 1 : 2000 dilution for 1 h.
T3381 6232-6406 Sentence denotes Blots were incubated with enhanced chemiluminescence (ECL) detection system (Amersham Biosciences, Bucks, UK) and exposed to radiographic film (Fuji Hyperfilm, Tokyo, Japan).
T91 6232-6406 Sentence denotes Blots were incubated with enhanced chemiluminescence (ECL) detection system (Amersham Biosciences, Bucks, UK) and exposed to radiographic film (Fuji Hyperfilm, Tokyo, Japan).
T3382 6407-6461 Sentence denotes ImageJ was used to analyze the intensity of the blots.
T92 6407-6461 Sentence denotes ImageJ was used to analyze the intensity of the blots.
T3383 6462-6513 Sentence denotes The level of β-actin was used as internal standard.
T93 6462-6513 Sentence denotes The level of β-actin was used as internal standard.
T4052 6515-6519 Sentence denotes 2.7.
T94 6515-6519 Sentence denotes 2.7.
T4053 6520-6561 Sentence denotes Enzyme-Linked Immunosorbent Assay (ELISA)
T95 6520-6561 Sentence denotes Enzyme-Linked Immunosorbent Assay (ELISA)
T4054 6562-6619 Sentence denotes Cells of four groups were homogenized as mentioned above.
T96 6562-6619 Sentence denotes Cells of four groups were homogenized as mentioned above.
T4055 6620-6742 Sentence denotes The supernatant was collected, and total protein was determined by Coomassie Plus Protein Assay Reagent (Pierce, IL, USA).
T97 6620-6742 Sentence denotes The supernatant was collected, and total protein was determined by Coomassie Plus Protein Assay Reagent (Pierce, IL, USA).
T98 6743-6971 Sentence denotes Levels of tumor necrosis factor-alpha (TNF-α), interleukin-1 beta (IL-1β), and interleukin-6 (IL-6) protein were quantified using ELISA kits specific for mouse according to the manufacturer's instructions (Bender MedSystems Inc.
T4056 6743-6981 Sentence denotes Levels of tumor necrosis factor-alpha (TNF-α), interleukin-1 beta (IL-1β), and interleukin-6 (IL-6) protein were quantified using ELISA kits specific for mouse according to the manufacturer's instructions (Bender MedSystems Inc. CA, USA).
T99 6972-6981 Sentence denotes CA, USA).
T4057 6982-7075 Sentence denotes Briefly, prepared the standard and created the standard dilution for building standard curve.
T100 6982-7075 Sentence denotes Briefly, prepared the standard and created the standard dilution for building standard curve.
T4058 7076-7140 Sentence denotes Then samples and biotinconjugate were added to microwell strips.
T101 7076-7140 Sentence denotes Then samples and biotinconjugate were added to microwell strips.
T4059 7141-7290 Sentence denotes After incubated for 2 h at room temperature, the microwell strips were washed 3 times with wash buffer, and streptavidin-HRP were added to all wells.
T102 7141-7290 Sentence denotes After incubated for 2 h at room temperature, the microwell strips were washed 3 times with wash buffer, and streptavidin-HRP were added to all wells.
T4060 7291-7386 Sentence denotes After incubated for 1 h, microwell strips were washed 3 times followed by adding TMB substrate.
T103 7291-7386 Sentence denotes After incubated for 1 h, microwell strips were washed 3 times followed by adding TMB substrate.
T4061 7387-7452 Sentence denotes After incubated for about 10–30 min, the stop solution was added.
T104 7387-7452 Sentence denotes After incubated for about 10–30 min, the stop solution was added.
T4062 7453-7569 Sentence denotes The colour intensity was measured at 450 nm using a Bio-Rad ELISA microplate reader (Bio-Rad Laboratories, CA, USA).
T105 7453-7569 Sentence denotes The colour intensity was measured at 450 nm using a Bio-Rad ELISA microplate reader (Bio-Rad Laboratories, CA, USA).
T4063 7570-7686 Sentence denotes The concentration of protein was determined according to the standard curve and expressed as pg/mg of total protein.
T106 7570-7686 Sentence denotes The concentration of protein was determined according to the standard curve and expressed as pg/mg of total protein.
T4378 7688-7692 Sentence denotes 2.8.
T107 7688-7692 Sentence denotes 2.8.
T4379 7693-7712 Sentence denotes Gelatine Zymography
T108 7693-7712 Sentence denotes Gelatine Zymography
T109 7713-7858 Sentence denotes Cells of four groups were homogenized in lysis buffer containing 50 mM Tris-HCl (pH 7.4), 150 mM NaCl, 5 mM CaCl2, 0.2 mM NaN3, and 0.01% Triton.
T4380 7713-8042 Sentence denotes Cells of four groups were homogenized in lysis buffer containing 50 mM Tris-HCl (pH 7.4), 150 mM NaCl, 5 mM CaCl2, 0.2 mM NaN3, and 0.01% Triton. Soluble extracts were separated by centrifugation and stored at –20°C. Gelatin zymography was performed according to the manufacturer's instructions (Genmed Scientifics Inc, MA, USA).
T110 7859-7929 Sentence denotes Soluble extracts were separated by centrifugation and stored at –20°C.
T111 7930-8042 Sentence denotes Gelatin zymography was performed according to the manufacturer's instructions (Genmed Scientifics Inc, MA, USA).
T4381 8043-8119 Sentence denotes Briefly, 40 μg cytosolic protein extracts were separated by electrophoresis.
T112 8043-8119 Sentence denotes Briefly, 40 μg cytosolic protein extracts were separated by electrophoresis.
T4382 8120-8285 Sentence denotes Then the proteins were renatured by incubation in 2.5% Triton X-100 and then incubated in substrate buffer for 40 h at 37°C to enable the MMP9 to cleave the gelatin.
T113 8120-8285 Sentence denotes Then the proteins were renatured by incubation in 2.5% Triton X-100 and then incubated in substrate buffer for 40 h at 37°C to enable the MMP9 to cleave the gelatin.
T4383 8286-8389 Sentence denotes After rinsing in water, each gel was stained with Coomassie blue for 1 h and destained in 50% methanol.
T114 8286-8389 Sentence denotes After rinsing in water, each gel was stained with Coomassie blue for 1 h and destained in 50% methanol.
T4384 8390-8495 Sentence denotes Proteolytic activities were showed by clear bands in blue gel which indicates the lysis of the substrate.
T115 8390-8495 Sentence denotes Proteolytic activities were showed by clear bands in blue gel which indicates the lysis of the substrate.
T4385 8496-8581 Sentence denotes Quantification of MMP9 band density was performed with image analysis program ImageJ.
T116 8496-8581 Sentence denotes Quantification of MMP9 band density was performed with image analysis program ImageJ.
T4757 8583-8587 Sentence denotes 2.9.
T117 8583-8587 Sentence denotes 2.9.
T4758 8588-8608 Sentence denotes Statistical Analysis
T118 8588-8608 Sentence denotes Statistical Analysis
T4759 8609-8698 Sentence denotes Data were expressed as mean ± SD and evaluated by ANOVA and LSD multiple comparison test.
T119 8609-8698 Sentence denotes Data were expressed as mean ± SD and evaluated by ANOVA and LSD multiple comparison test.
T4760 8699-8748 Sentence denotes P values <0.05 were considered to be significant.
T120 8699-8748 Sentence denotes P values <0.05 were considered to be significant.
T4761 8749-8805 Sentence denotes All analyses were performed by using SPSS 18.0 software.
T121 8749-8805 Sentence denotes All analyses were performed by using SPSS 18.0 software.