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PMC:3317373 / 10541-11434 JSONTXT 19 Projects

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Id Subject Object Predicate Lexical cue
T4378 0-4 Sentence denotes 2.8.
T107 0-4 Sentence denotes 2.8.
T4379 5-24 Sentence denotes Gelatine Zymography
T108 5-24 Sentence denotes Gelatine Zymography
T109 25-170 Sentence denotes Cells of four groups were homogenized in lysis buffer containing 50 mM Tris-HCl (pH 7.4), 150 mM NaCl, 5 mM CaCl2, 0.2 mM NaN3, and 0.01% Triton.
T4380 25-354 Sentence denotes Cells of four groups were homogenized in lysis buffer containing 50 mM Tris-HCl (pH 7.4), 150 mM NaCl, 5 mM CaCl2, 0.2 mM NaN3, and 0.01% Triton. Soluble extracts were separated by centrifugation and stored at –20°C. Gelatin zymography was performed according to the manufacturer's instructions (Genmed Scientifics Inc, MA, USA).
T110 171-241 Sentence denotes Soluble extracts were separated by centrifugation and stored at –20°C.
T111 242-354 Sentence denotes Gelatin zymography was performed according to the manufacturer's instructions (Genmed Scientifics Inc, MA, USA).
T4381 355-431 Sentence denotes Briefly, 40 μg cytosolic protein extracts were separated by electrophoresis.
T112 355-431 Sentence denotes Briefly, 40 μg cytosolic protein extracts were separated by electrophoresis.
T4382 432-597 Sentence denotes Then the proteins were renatured by incubation in 2.5% Triton X-100 and then incubated in substrate buffer for 40 h at 37°C to enable the MMP9 to cleave the gelatin.
T113 432-597 Sentence denotes Then the proteins were renatured by incubation in 2.5% Triton X-100 and then incubated in substrate buffer for 40 h at 37°C to enable the MMP9 to cleave the gelatin.
T4383 598-701 Sentence denotes After rinsing in water, each gel was stained with Coomassie blue for 1 h and destained in 50% methanol.
T114 598-701 Sentence denotes After rinsing in water, each gel was stained with Coomassie blue for 1 h and destained in 50% methanol.
T4384 702-807 Sentence denotes Proteolytic activities were showed by clear bands in blue gel which indicates the lysis of the substrate.
T115 702-807 Sentence denotes Proteolytic activities were showed by clear bands in blue gel which indicates the lysis of the substrate.
T4385 808-893 Sentence denotes Quantification of MMP9 band density was performed with image analysis program ImageJ.
T116 808-893 Sentence denotes Quantification of MMP9 band density was performed with image analysis program ImageJ.