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PMC:3312845 / 11631-13094 JSONTXT 22 Projects

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Id Subject Object Predicate Lexical cue
T5369 0-31 Sentence denotes Double immunofluorescence study
T91 0-31 Sentence denotes Double immunofluorescence study
T5370 32-123 Sentence denotes Sections were incubated with 3% bovine serum albumin in PBS for 30 min at room temperature.
T92 32-123 Sentence denotes Sections were incubated with 3% bovine serum albumin in PBS for 30 min at room temperature.
T5371 124-959 Sentence denotes Sections were then incubated in a mixture of goat anti-TNF-α IgG (1:1000, R&D systems, Minneapolis, MN)/mouse anti-OX-42 IgG (1:100, Serotec, Cambridge, UK), mouse anti-GFAP IgG (1:1000, an astroglial marker, Millipore Corporation, Billerica, MA)/rabbit anti-TNFp55R IgG (1:1000, Abcam, Cambridge, UK), mouse anti-GFAP IgG/rabbit anti-TNFp75R IgG (1:1000, Abcam, Cambridge, UK), mouse anti-SMI-71 IgM (1:1000, Covance, Berkeley, CA)/rabbit anti-TNFp75R IgG, mouse anti-GFAP IgG/rabbit anti-NF-κB (p65-Ser276, p65-Ser311, p65-Ser529, and p65-Thr435) IgG (1:100, Abcam, Cambridge, UK), mouse anti-SMI-71 IgM/rabbit anti-p65-Thr435 NF-κB IgG, mouse anti-SMI-71 IgM/rabbit anti-GLUT-1 IgG (1:100, Abcam, Cambridge, UK), or mouse anti-GFAP IgG/rabbit anti-MIP-2 IgG (1:100) in PBS containing 0.3% triton X-100 overnight at room temperature.
T93 124-959 Sentence denotes Sections were then incubated in a mixture of goat anti-TNF-α IgG (1:1000, R&D systems, Minneapolis, MN)/mouse anti-OX-42 IgG (1:100, Serotec, Cambridge, UK), mouse anti-GFAP IgG (1:1000, an astroglial marker, Millipore Corporation, Billerica, MA)/rabbit anti-TNFp55R IgG (1:1000, Abcam, Cambridge, UK), mouse anti-GFAP IgG/rabbit anti-TNFp75R IgG (1:1000, Abcam, Cambridge, UK), mouse anti-SMI-71 IgM (1:1000, Covance, Berkeley, CA)/rabbit anti-TNFp75R IgG, mouse anti-GFAP IgG/rabbit anti-NF-κB (p65-Ser276, p65-Ser311, p65-Ser529, and p65-Thr435) IgG (1:100, Abcam, Cambridge, UK), mouse anti-SMI-71 IgM/rabbit anti-p65-Thr435 NF-κB IgG, mouse anti-SMI-71 IgM/rabbit anti-GLUT-1 IgG (1:100, Abcam, Cambridge, UK), or mouse anti-GFAP IgG/rabbit anti-MIP-2 IgG (1:100) in PBS containing 0.3% triton X-100 overnight at room temperature.
T5372 960-1175 Sentence denotes After washing three times for 10 minutes with PBS, sections were also incubated in a mixture of FITC- and Cy3-conjugated secondary antisera (Amersham, San Francisco, CA), diluted 1:200, for 2 hr at room temperature.
T94 960-1175 Sentence denotes After washing three times for 10 minutes with PBS, sections were also incubated in a mixture of FITC- and Cy3-conjugated secondary antisera (Amersham, San Francisco, CA), diluted 1:200, for 2 hr at room temperature.
T5373 1176-1271 Sentence denotes The sections were washed three times for 10 min with PBS, and mounted on gelatin-coated slides.
T95 1176-1271 Sentence denotes The sections were washed three times for 10 min with PBS, and mounted on gelatin-coated slides.
T5374 1272-1371 Sentence denotes For nuclei counterstaining, we used Vectashield mounting medium with DAPI (Vector, Burlingame, CA).
T96 1272-1371 Sentence denotes For nuclei counterstaining, we used Vectashield mounting medium with DAPI (Vector, Burlingame, CA).
T97 1372-1449 Sentence denotes All images were captured using an AxioImage M2 microscope and AxioVision Rel.
T5375 1372-1463 Sentence denotes All images were captured using an AxioImage M2 microscope and AxioVision Rel. 4.8 software.
T98 1450-1463 Sentence denotes 4.8 software.