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PMC:3279418 / 31854-32537 JSONTXT 12 Projects

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Id Subject Object Predicate Lexical cue
T12083 0-18 Sentence denotes Immunofluorescence
T225 0-18 Sentence denotes Immunofluorescence
T12084 19-87 Sentence denotes Fibroblast and macrophage cells were transfected with pFLAG-SHARPIN.
T226 19-87 Sentence denotes Fibroblast and macrophage cells were transfected with pFLAG-SHARPIN.
T12085 88-231 Sentence denotes 48 hours later, cells were washed briefly with PBS, then fixed in cold methanol at −20°C for 10 minutes and cold acetone at -20°C for 1 minute.
T227 88-231 Sentence denotes 48 hours later, cells were washed briefly with PBS, then fixed in cold methanol at −20°C for 10 minutes and cold acetone at -20°C for 1 minute.
T12086 232-308 Sentence denotes Incubate cells with PBS+1% BSA for 15 minutes to block non-specific binding.
T228 232-308 Sentence denotes Incubate cells with PBS+1% BSA for 15 minutes to block non-specific binding.
T12087 309-454 Sentence denotes Fixed cells were then incubated with anti-FLAG (1∶100) as the primary antibody at room temperature for 1 hour, followed by three 5-minute washes.
T229 309-454 Sentence denotes Fixed cells were then incubated with anti-FLAG (1∶100) as the primary antibody at room temperature for 1 hour, followed by three 5-minute washes.
T12088 455-619 Sentence denotes Cells were further incubated with goat anti-rabbit IgG-FITC (1∶100) as the secondary antibody at room temperature for 30 minutes, followed by three 5-minute washes.
T230 455-619 Sentence denotes Cells were further incubated with goat anti-rabbit IgG-FITC (1∶100) as the secondary antibody at room temperature for 30 minutes, followed by three 5-minute washes.
T12089 620-683 Sentence denotes Cells were then examined using inverse fluorescence microscope.
T231 620-683 Sentence denotes Cells were then examined using inverse fluorescence microscope.