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PMC:3279418 / 30214-31448 JSONTXT 15 Projects

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Id Subject Object Predicate Lexical cue
T11351 0-22 Sentence denotes RNA expression by BMDC
T211 0-22 Sentence denotes RNA expression by BMDC
T212 23-161 Sentence denotes BMDC were cultured at 106 cells/mL in 10 mL of RPMI-1640 complete medium in the presence or absence of 100 ng/mL LPS or 25 µg/mL poly I:C.
T11352 23-270 Sentence denotes BMDC were cultured at 106 cells/mL in 10 mL of RPMI-1640 complete medium in the presence or absence of 100 ng/mL LPS or 25 µg/mL poly I:C. After 1 and 2 hours, RNA was isolated with TRI-reagent (Sigma) according to the manufacturer's instructions.
T213 162-270 Sentence denotes After 1 and 2 hours, RNA was isolated with TRI-reagent (Sigma) according to the manufacturer's instructions.
T11353 271-353 Sentence denotes The expression of Il6, Il12p40, Gmcsf, and Sharpin mRNA was determined by qRT-PCR.
T214 271-353 Sentence denotes The expression of Il6, Il12p40, Gmcsf, and Sharpin mRNA was determined by qRT-PCR.
T215 354-412 Sentence denotes Primers and probes were purchased from Applied Biosystems.
T11354 354-756 Sentence denotes Primers and probes were purchased from Applied Biosystems. Reverse transcription was performed at 42°C for 60 minutes with the final denaturation step at 90°C for 5 minutes in 30 µl containing 0.5 µg of total RNA. dNTPs, oligo(dT)15 primer, recombinant RNasin Ribonuclease Inhibitor, and M-MLV Reverse Transcriptase (all from Promega, Madison, WI) were used according to the manufacturer's instruction.
T216 413-756 Sentence denotes Reverse transcription was performed at 42°C for 60 minutes with the final denaturation step at 90°C for 5 minutes in 30 µl containing 0.5 µg of total RNA. dNTPs, oligo(dT)15 primer, recombinant RNasin Ribonuclease Inhibitor, and M-MLV Reverse Transcriptase (all from Promega, Madison, WI) were used according to the manufacturer's instruction.
T11355 757-1090 Sentence denotes Reverse transcription was done in a PTC-200 Peltier Thermal Cycler (MJ Research, Watertown, MA). qRT-PCR was performed in ABI Prism 7700 Sequence Detection System with TaqMan® Gene Expression Assays (Applied Biosystems, Foster City, CA) for mouse Actb, IL6, IL12p40, Ifnβ, Gmcsf, and Sharpin according to the manufacturer's protocol.
T217 757-1090 Sentence denotes Reverse transcription was done in a PTC-200 Peltier Thermal Cycler (MJ Research, Watertown, MA). qRT-PCR was performed in ABI Prism 7700 Sequence Detection System with TaqMan® Gene Expression Assays (Applied Biosystems, Foster City, CA) for mouse Actb, IL6, IL12p40, Ifnβ, Gmcsf, and Sharpin according to the manufacturer's protocol.
T11356 1091-1164 Sentence denotes The endogenous standard for normalization of the target gene was β-actin.
T218 1091-1164 Sentence denotes The endogenous standard for normalization of the target gene was β-actin.
T11357 1165-1234 Sentence denotes Relative gene expression was calculated using the 2−ΔΔCt method [56].
T219 1165-1234 Sentence denotes Relative gene expression was calculated using the 2−ΔΔCt method [56].