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PMC:3279418 / 16693-19345 JSONTXT 16 Projects

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Id Subject Object Predicate Lexical cue
T5820 0-49 Sentence denotes Th2-biased immunogenicity of stimulated cpdm BMDC
T113 0-49 Sentence denotes Th2-biased immunogenicity of stimulated cpdm BMDC
T5821 50-263 Sentence denotes The defective IL12 production (Fig. 3A) and Th2-dominant cytokine profile in cpdm mice [5] suggest that the absence of SHARPIN affects the ability of cpdm BMDC to induce T cell differentiation into effector cells.
T114 50-263 Sentence denotes The defective IL12 production (Fig. 3A) and Th2-dominant cytokine profile in cpdm mice [5] suggest that the absence of SHARPIN affects the ability of cpdm BMDC to induce T cell differentiation into effector cells.
T5822 264-564 Sentence denotes Co-culture of allogeneic naïve CD4+ T cells with WT BMDC stimulated with LPS or poly I:C elicited robust IFNγ production, whereas the concentration of IFNγ in cpdm BMDC-T cell cultures was significantly lower after LPS stimulation (Fig. 7A), indicating impaired Th1-polarizing abilities of cpdm BMDC.
T115 264-564 Sentence denotes Co-culture of allogeneic naïve CD4+ T cells with WT BMDC stimulated with LPS or poly I:C elicited robust IFNγ production, whereas the concentration of IFNγ in cpdm BMDC-T cell cultures was significantly lower after LPS stimulation (Fig. 7A), indicating impaired Th1-polarizing abilities of cpdm BMDC.
T5823 565-701 Sentence denotes In addition to TLR3/4 agonists, the TLR2 ligand Pam3CYS was used since it has been shown to induce both Th1 and Th2 responses [28]–[30].
T116 565-701 Sentence denotes In addition to TLR3/4 agonists, the TLR2 ligand Pam3CYS was used since it has been shown to induce both Th1 and Th2 responses [28]–[30].
T5824 702-814 Sentence denotes Pam3CYS-matured WT BMDC induced robust IFNγ production at a significantly higher level than cpdm BMDC (Fig. 7A).
T117 702-814 Sentence denotes Pam3CYS-matured WT BMDC induced robust IFNγ production at a significantly higher level than cpdm BMDC (Fig. 7A).
T5825 815-995 Sentence denotes The reduced Th1 differentiation following Pam3CYS stimulation is consistent with the recent report of decreased IL12 production in cpdm macrophages following TLR2 stimulation [31].
T118 815-995 Sentence denotes The reduced Th1 differentiation following Pam3CYS stimulation is consistent with the recent report of decreased IL12 production in cpdm macrophages following TLR2 stimulation [31].
T5826 996-1163 Sentence denotes In contrast, more Th2-specific IL4 cytokine was produced in the cpdm BMDC co-cultures than the WT control (Fig. 7B), suggesting Th2-skewed immunogenicity of cpdm BMDC.
T119 996-1163 Sentence denotes In contrast, more Th2-specific IL4 cytokine was produced in the cpdm BMDC co-cultures than the WT control (Fig. 7B), suggesting Th2-skewed immunogenicity of cpdm BMDC.
T5827 1164-1332 Sentence denotes The production of Th17-specific cytokine IL17A following LPS stimulation of dendritic cells was similar between stimulated WT and cpdm BMDC cocultures (data not shown).
T120 1164-1332 Sentence denotes The production of Th17-specific cytokine IL17A following LPS stimulation of dendritic cells was similar between stimulated WT and cpdm BMDC cocultures (data not shown).
T5828 1333-1573 Sentence denotes Despite the distinct Th1- and Th2-stimulating abilities of WT and cpdm BMDC, they were equally effective in IL2 production from BMDC-T cell co-cultures except for poly I:C stimulation where WT BMDC induced more IL2 than cpdm BMDC (Fig. 7C).
T121 1333-1573 Sentence denotes Despite the distinct Th1- and Th2-stimulating abilities of WT and cpdm BMDC, they were equally effective in IL2 production from BMDC-T cell co-cultures except for poly I:C stimulation where WT BMDC induced more IL2 than cpdm BMDC (Fig. 7C).
T5829 1574-1754 Sentence denotes The Th2-biased stimulating capability of cpdm BMDC when co-cultured with allogeneic naïve CD4+ T cells is consistent with the Th2-dominant cytokine phenotype observed in cpdm mice.
T122 1574-1754 Sentence denotes The Th2-biased stimulating capability of cpdm BMDC when co-cultured with allogeneic naïve CD4+ T cells is consistent with the Th2-dominant cytokine phenotype observed in cpdm mice.
T123 1755-1883 Sentence denotes 10.1371/journal.pone.0031809.g007 Figure 7 Stimulated cpdm BMDC induced Th2-biased cytokine production from naïve CD4+ T cells.
T15334 1799-1883 Sentence denotes Stimulated cpdm BMDC induced Th2-biased cytokine production from naïve CD4+ T cells.
T15335 1884-2042 Sentence denotes WT and cpdm BMDC (5×104 cells in 0.1 mL complete medium; MHC haplotype: H-2b) were incubated with medium, 100 ng/mL LPS, 25 µg/mL poly I:C or 5 µg/mL Pam3CYS.
T124 1884-2042 Sentence denotes WT and cpdm BMDC (5×104 cells in 0.1 mL complete medium; MHC haplotype: H-2b) were incubated with medium, 100 ng/mL LPS, 25 µg/mL poly I:C or 5 µg/mL Pam3CYS.
T15336 2043-2215 Sentence denotes After 24 hours, cells were washed with PBS and incubated with freshly isolated allogeneic naïve CD4+ T cells (2.5×105 cells in 0.1 mL complete medium; MHC haplotype: H-2d).
T125 2043-2215 Sentence denotes After 24 hours, cells were washed with PBS and incubated with freshly isolated allogeneic naïve CD4+ T cells (2.5×105 cells in 0.1 mL complete medium; MHC haplotype: H-2d).
T15337 2216-2321 Sentence denotes After 5 days, supernatants were collected and the secretion of IFNγ, IL4, and IL2 were measured by ELISA.
T126 2216-2321 Sentence denotes After 5 days, supernatants were collected and the secretion of IFNγ, IL4, and IL2 were measured by ELISA.
T15338 2322-2479 Sentence denotes Negative controls are 1) stimulated BMDC without co-culture with allogeneic CD4+ T cells; 2) allogeneic CD4+ T cells without co-culture with stimulated BMDC.
T127 2322-2479 Sentence denotes Negative controls are 1) stimulated BMDC without co-culture with allogeneic CD4+ T cells; 2) allogeneic CD4+ T cells without co-culture with stimulated BMDC.
T15339 2480-2589 Sentence denotes Samples from both negative controls had no detectable production of the aforementioned cytokines (not shown).
T128 2480-2589 Sentence denotes Samples from both negative controls had no detectable production of the aforementioned cytokines (not shown).
T15340 2590-2649 Sentence denotes Results are analyzed based on 2–4 mice per group. * P<0.05.
T129 2590-2649 Sentence denotes Results are analyzed based on 2–4 mice per group. * P<0.05.