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PMC:2817507 / 7770-8455 JSONTXT 23 Projects

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Id Subject Object Predicate Lexical cue
T3718 0-186 Sentence denotes Cellular proteins were prepared from ICH cerebral hemispheres, electrophoresed with SDS-polyacrylamide gel and transferred onto Hybond ECL membranes (Amersham Pharmacia, Piscataway, NJ).
T79 0-186 Sentence denotes Cellular proteins were prepared from ICH cerebral hemispheres, electrophoresed with SDS-polyacrylamide gel and transferred onto Hybond ECL membranes (Amersham Pharmacia, Piscataway, NJ).
T3719 187-370 Sentence denotes The ECL membranes were incubated with the appropriate primary antibody (WuHan, BA1717) followed by incubation with peroxidase-conjugated secondary antibodies (Golden Bridge, PV-6001).
T80 187-370 Sentence denotes The ECL membranes were incubated with the appropriate primary antibody (WuHan, BA1717) followed by incubation with peroxidase-conjugated secondary antibodies (Golden Bridge, PV-6001).
T3720 371-438 Sentence denotes The signals were detected with the ECL system (Amersham Pharmacia).
T81 371-438 Sentence denotes The signals were detected with the ECL system (Amersham Pharmacia).
T3721 439-594 Sentence denotes The same membranes were probed with anti-GAPDH (glyceraldehyde-3-phosphate dehydrogenase, Biodesign, Saco, Maine) after being washed with stripping buffer.
T82 439-594 Sentence denotes The same membranes were probed with anti-GAPDH (glyceraldehyde-3-phosphate dehydrogenase, Biodesign, Saco, Maine) after being washed with stripping buffer.
T3722 595-685 Sentence denotes The signals were quantified by scanning densitometry and computer-assisted image analysis.
T83 595-685 Sentence denotes The signals were quantified by scanning densitometry and computer-assisted image analysis.