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PMC:2817507 / 5816-7332 JSONTXT 21 Projects

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Id Subject Object Predicate Lexical cue
T2729 0-4 Sentence denotes 2.3.
T51 0-4 Sentence denotes 2.3.
T2730 5-51 Sentence denotes Detection of TLR4 mRNA by the Method of RT-PCR
T52 5-51 Sentence denotes Detection of TLR4 mRNA by the Method of RT-PCR
T2731 52-249 Sentence denotes Briefly, total RNA samples were isolated from peri-hemorrhage regions in left basal ganglia using Simply P Total RNA isolation Kit (BioFlux, HZ, China) according to the manufacturer's instructions.
T53 52-249 Sentence denotes Briefly, total RNA samples were isolated from peri-hemorrhage regions in left basal ganglia using Simply P Total RNA isolation Kit (BioFlux, HZ, China) according to the manufacturer's instructions.
T2732 250-393 Sentence denotes The quantity of total RNA was determined on a Nanodrop (ND-1000 Spectrophotometer) by measuring optical density at A260 and A280 nm wavelength.
T54 250-393 Sentence denotes The quantity of total RNA was determined on a Nanodrop (ND-1000 Spectrophotometer) by measuring optical density at A260 and A280 nm wavelength.
T2733 394-453 Sentence denotes Then, the concentrations of mRNA were adjusted to 1.0 g/mL.
T55 394-453 Sentence denotes Then, the concentrations of mRNA were adjusted to 1.0 g/mL.
T2734 454-630 Sentence denotes RNA was reverse-transcribed into cDNA in 20 μL reaction system using Superscript First-Strand Synthesis Kit for RT-PCR (promega Inc) under conditions described by the supplier.
T56 454-630 Sentence denotes RNA was reverse-transcribed into cDNA in 20 μL reaction system using Superscript First-Strand Synthesis Kit for RT-PCR (promega Inc) under conditions described by the supplier.
T2735 631-797 Sentence denotes One microliter of cDNA was used to amplify the TLR4 gene fragment with 1× PCR buffer, 1.5 mM MgCl2, 200 μM of each dNTP, 200 μM of primers, and 2u Taq DNA polymerase.
T57 631-797 Sentence denotes One microliter of cDNA was used to amplify the TLR4 gene fragment with 1× PCR buffer, 1.5 mM MgCl2, 200 μM of each dNTP, 200 μM of primers, and 2u Taq DNA polymerase.
T2736 798-860 Sentence denotes The house keep gene (β-actin) was used as an internal control.
T58 798-860 Sentence denotes The house keep gene (β-actin) was used as an internal control.
T2737 861-969 Sentence denotes The PCR primer sequences were designed according to the TLR4 and β-actin gene sequences reported in GenBank.
T59 861-969 Sentence denotes The PCR primer sequences were designed according to the TLR4 and β-actin gene sequences reported in GenBank.
T2738 970-993 Sentence denotes TLR4 (145 bp): forward:
T60 970-993 Sentence denotes TLR4 (145 bp): forward:
T2739 994-1030 Sentence denotes 5′-AGCTTTGGTCAGTTGGCTCT-3′; reverse:
T61 994-1030 Sentence denotes 5′-AGCTTTGGTCAGTTGGCTCT-3′; reverse:
T2740 1031-1085 Sentence denotes 5′-CAGGATGACACCATTGAAGC-3′; β-actin (701 bp): forward:
T62 1031-1085 Sentence denotes 5′-CAGGATGACACCATTGAAGC-3′; β-actin (701 bp): forward:
T2741 1086-1121 Sentence denotes 5′-GCCAACCGTGAAAAAGATG-3′; reverse:
T63 1086-1121 Sentence denotes 5′-GCCAACCGTGAAAAAGATG-3′; reverse:
T2742 1122-1148 Sentence denotes 5′-CCAGGATAGAGCCACCAAT-3′.
T64 1122-1148 Sentence denotes 5′-CCAGGATAGAGCCACCAAT-3′.
T2743 1149-1207 Sentence denotes Reaction cycles were performed at the following condition:
T65 1149-1207 Sentence denotes Reaction cycles were performed at the following condition:
T2744 1208-1256 Sentence denotes 94°C 2 min, 94°C 40 s, 55°C 40 s, and 72°C 60 s.
T66 1208-1256 Sentence denotes 94°C 2 min, 94°C 40 s, 55°C 40 s, and 72°C 60 s.
T2745 1257-1298 Sentence denotes The reaction was stopped after 31 cycles.
T67 1257-1298 Sentence denotes The reaction was stopped after 31 cycles.
T2746 1299-1353 Sentence denotes PCR products were electrophoresed through agarose gel.
T68 1299-1353 Sentence denotes PCR products were electrophoresed through agarose gel.
T2747 1354-1432 Sentence denotes Image Acquisition and Analysis software were used to determine band densities.
T69 1354-1432 Sentence denotes Image Acquisition and Analysis software were used to determine band densities.
T2748 1433-1516 Sentence denotes The abundance of TLR4 mRNA was expressed as the ratio of TLR4 mRNA to β-actin mRNA.
T70 1433-1516 Sentence denotes The abundance of TLR4 mRNA was expressed as the ratio of TLR4 mRNA to β-actin mRNA.