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PMC:1310901 / 5973-6808 JSONTXT 14 Projects

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Id Subject Object Predicate Lexical cue
T3053 0-32 Sentence denotes Sequencing of the IRF-4 promoter
T42 0-32 Sentence denotes Sequencing of the IRF-4 promoter
T3054 33-299 Sentence denotes For analysis of the IRF-4 promoter region for permanent aberrations such as insertions/deletions or mutation, we PCR-amplified two fragments from genomic DNA, which was extracted from depicted cell lines with a commercial kit (Qiagen, Hilde, Germany) as recommended.
T43 33-299 Sentence denotes For analysis of the IRF-4 promoter region for permanent aberrations such as insertions/deletions or mutation, we PCR-amplified two fragments from genomic DNA, which was extracted from depicted cell lines with a commercial kit (Qiagen, Hilde, Germany) as recommended.
T3055 300-327 Sentence denotes The primers were 1-forward:
T44 300-327 Sentence denotes The primers were 1-forward:
T3056 328-368 Sentence denotes 5′-TTGAGATGGAGTCTTGCTCTGT-3′, 1-reverse:
T45 328-368 Sentence denotes 5′-TTGAGATGGAGTCTTGCTCTGT-3′, 1-reverse:
T3057 369-410 Sentence denotes 5′-CCAGGACCTCAGGAGGCCAGTCA-3′; 2-forward:
T46 369-410 Sentence denotes 5′-CCAGGACCTCAGGAGGCCAGTCA-3′; 2-forward:
T3058 411-454 Sentence denotes 5′-AGCGGTGAAACTGAGAGTGCGAGGT-3′, 2-reverse:
T47 411-454 Sentence denotes 5′-AGCGGTGAAACTGAGAGTGCGAGGT-3′, 2-reverse:
T3059 455-482 Sentence denotes 5′-GCCACATCGCTGCAGTTTAG-3′.
T48 455-482 Sentence denotes 5′-GCCACATCGCTGCAGTTTAG-3′.
T3060 483-580 Sentence denotes The products were cloned with the ‘TOPO TA cloning kit’ (Invitrogen, Groningen, The Netherlands).
T49 483-580 Sentence denotes The products were cloned with the ‘TOPO TA cloning kit’ (Invitrogen, Groningen, The Netherlands).
T3061 581-835 Sentence denotes After bacterial amplification of the cloned PCR fragments by standard procedures, at least three clones from each sample were sequenced with an automated sequencer (ABI Prism 377, Applied Bio-systems, Foster City, USA) as recommended by the manufacturer.
T50 581-835 Sentence denotes After bacterial amplification of the cloned PCR fragments by standard procedures, at least three clones from each sample were sequenced with an automated sequencer (ABI Prism 377, Applied Bio-systems, Foster City, USA) as recommended by the manufacturer.