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PMC:1310901 / 14326-15970 JSONTXT 16 Projects

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Id Subject Object Predicate Lexical cue
T7004 0-81 Sentence denotes Increase of IRF-4 expression in hematopoietic cells after demethylating treatment
T108 0-81 Sentence denotes Increase of IRF-4 expression in hematopoietic cells after demethylating treatment
T7005 82-189 Sentence denotes We next analyzed whether promoter methylation could be responsible for down-regulation of IRF-4 expression.
T109 82-189 Sentence denotes We next analyzed whether promoter methylation could be responsible for down-regulation of IRF-4 expression.
T7006 190-296 Sentence denotes A region including exon1 in the IRF-4 promoter exhibited a large number of CpG-rich sequences (Figure 3A).
T110 190-296 Sentence denotes A region including exon1 in the IRF-4 promoter exhibited a large number of CpG-rich sequences (Figure 3A).
T7007 297-493 Sentence denotes Several chemical substances such as 5-aza-2-deoxycytidine (AzadC) or 5-azacytidine (AzaC) inhibit de novo and maintenance methylation, and thus can be used to discern promoter methylation (32,33).
T111 297-493 Sentence denotes Several chemical substances such as 5-aza-2-deoxycytidine (AzadC) or 5-azacytidine (AzaC) inhibit de novo and maintenance methylation, and thus can be used to discern promoter methylation (32,33).
T7008 494-537 Sentence denotes We used AzadC to generate unmethylated DNA.
T112 494-537 Sentence denotes We used AzadC to generate unmethylated DNA.
T7009 538-721 Sentence denotes A 72 h AzadC-treatment resulted in a concentration-dependent activation of IRF-4 transcription in Jurkat and CML-T1 T-cells as well as in U-937, K-562 and EM-2 cell lines (Figure 2A).
T113 538-721 Sentence denotes A 72 h AzadC-treatment resulted in a concentration-dependent activation of IRF-4 transcription in Jurkat and CML-T1 T-cells as well as in U-937, K-562 and EM-2 cell lines (Figure 2A).
T7010 722-889 Sentence denotes IRF-4 transcription was induced in a time-dependent manner and was observed as early as 24 h after treatment with AzadC and increased over time until 72 h (Figure 2B).
T114 722-889 Sentence denotes IRF-4 transcription was induced in a time-dependent manner and was observed as early as 24 h after treatment with AzadC and increased over time until 72 h (Figure 2B).
T7011 890-1040 Sentence denotes Time and strength of the appearance of IRF-4 transcripts varied among cell lines, i.e. CML-T1 responded strongest to AzadC-treatment (data not shown).
T115 890-1040 Sentence denotes Time and strength of the appearance of IRF-4 transcripts varied among cell lines, i.e. CML-T1 responded strongest to AzadC-treatment (data not shown).
T7012 1041-1176 Sentence denotes In line with this, AzadC-treatment of CML-T1 and LAMA-84 cells also translated in an induction of IRF-4 protein expression (Figure 2C).
T116 1041-1176 Sentence denotes In line with this, AzadC-treatment of CML-T1 and LAMA-84 cells also translated in an induction of IRF-4 protein expression (Figure 2C).
T7013 1177-1316 Sentence denotes Accordingly, treatment of the IRF-4-positive cell line BV-173, SD-1 and RPMI-8226 with AzadC had no effect on IRF-4 expression (Figure 2D).
T117 1177-1316 Sentence denotes Accordingly, treatment of the IRF-4-positive cell line BV-173, SD-1 and RPMI-8226 with AzadC had no effect on IRF-4 expression (Figure 2D).
T7014 1317-1458 Sentence denotes There was no difference in the effects of AzaC versus AzadC, as both increased the IRF-4 mRNA level in CML-T1 cells as well (data not shown).
T118 1317-1458 Sentence denotes There was no difference in the effects of AzaC versus AzadC, as both increased the IRF-4 mRNA level in CML-T1 cells as well (data not shown).
T7015 1459-1644 Sentence denotes This implied that promoter methylation may control IRF-4 expression, but an alternative explanation may be activation of positive transcriptional regulators of IRF-4 by AzadC (or AzaC).
T119 1459-1644 Sentence denotes This implied that promoter methylation may control IRF-4 expression, but an alternative explanation may be activation of positive transcriptional regulators of IRF-4 by AzadC (or AzaC).