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PMC:1310901 / 12057-13047 JSONTXT 17 Projects

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Id Subject Object Predicate Lexical cue
T5684 0-45 Sentence denotes In vitro methylation and reporter gene assays
T90 0-45 Sentence denotes In vitro methylation and reporter gene assays
T91 46-118 Sentence denotes The IRF-4 promoter-reporter gene construct was generously provided by J.
T5685 46-132 Sentence denotes The IRF-4 promoter-reporter gene construct was generously provided by J. Hiscott (31).
T92 119-132 Sentence denotes Hiscott (31).
T5686 133-320 Sentence denotes Constructs were methylated in vitro with CpG Methylase (M.Sss I) as recommended by the manufacturer (NE Biolabs) and complete methylation was checked via restriction analysis (Figure 5A).
T93 133-320 Sentence denotes Constructs were methylated in vitro with CpG Methylase (M.Sss I) as recommended by the manufacturer (NE Biolabs) and complete methylation was checked via restriction analysis (Figure 5A).
T5687 321-432 Sentence denotes Reporter gene assays using the dual luciferase assay (Promega) were performed similar to previous reports (29).
T94 321-432 Sentence denotes Reporter gene assays using the dual luciferase assay (Promega) were performed similar to previous reports (29).
T5688 433-600 Sentence denotes Briefly, 5 nM of the reporter construct and the transfection control construct expressing the renilla luciferase gene were transientlyco-expressed via electroporation.
T95 433-600 Sentence denotes Briefly, 5 nM of the reporter construct and the transfection control construct expressing the renilla luciferase gene were transientlyco-expressed via electroporation.
T5689 601-683 Sentence denotes The control construct served as an internal reference for transfection efficiency.
T96 601-683 Sentence denotes The control construct served as an internal reference for transfection efficiency.
T5690 684-819 Sentence denotes Forty-eight hours after transfection, luciferase activity was measured with a LB 96 P microlumat (EG&G Berthold, Bad Wildbad, Germany).
T97 684-819 Sentence denotes Forty-eight hours after transfection, luciferase activity was measured with a LB 96 P microlumat (EG&G Berthold, Bad Wildbad, Germany).
T5691 820-936 Sentence denotes IRF-4 promoter activation was quantified as a ratio of measured firefly light units (flu) relative to renilla (rlu).
T98 820-936 Sentence denotes IRF-4 promoter activation was quantified as a ratio of measured firefly light units (flu) relative to renilla (rlu).
T5692 937-990 Sentence denotes Each experiment was carried out at least three times.
T99 937-990 Sentence denotes Each experiment was carried out at least three times.