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PMC:1134658 / 7310-8478 JSONTXT 16 Projects

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Id Subject Object Predicate Lexical cue
T4599 0-42 Sentence denotes BMP-6 induces phosphorylation of Smad1/5/8
T60 0-42 Sentence denotes BMP-6 induces phosphorylation of Smad1/5/8
T4600 43-139 Sentence denotes Upon ligand binding, the type II receptor transphosphorylates and activates the type I receptor.
T61 43-139 Sentence denotes Upon ligand binding, the type II receptor transphosphorylates and activates the type I receptor.
T4601 140-189 Sentence denotes Type I receptors can signal via several pathways.
T62 140-189 Sentence denotes Type I receptors can signal via several pathways.
T4602 190-334 Sentence denotes We examined the effect of BMP-6 on Smad phosphorylation, as the activation of Smad is considered to be a major signalling pathway for BMPs [17].
T63 190-334 Sentence denotes We examined the effect of BMP-6 on Smad phosphorylation, as the activation of Smad is considered to be a major signalling pathway for BMPs [17].
T4603 335-440 Sentence denotes B cells were cultured in serum-free media over night and then treated with BMP-6 for various time points.
T64 335-440 Sentence denotes B cells were cultured in serum-free media over night and then treated with BMP-6 for various time points.
T4604 441-576 Sentence denotes Total protein lysates were prepared, and the amounts of the phosphorylated forms of Smad1/5/8 were determined by western blot analysis.
T65 441-576 Sentence denotes Total protein lysates were prepared, and the amounts of the phosphorylated forms of Smad1/5/8 were determined by western blot analysis.
T66 577-655 Sentence denotes Interestingly, treatment with 500 ng/ml BMP-6 induced phosphorylation of Smad.
T4605 577-798 Sentence denotes Interestingly, treatment with 500 ng/ml BMP-6 induced phosphorylation of Smad. The BMP-6 induced phosphorylation was high at the earliest time point tested (15 minutes), and remained high for at least 48 hours (Figure 5).
T67 656-798 Sentence denotes The BMP-6 induced phosphorylation was high at the earliest time point tested (15 minutes), and remained high for at least 48 hours (Figure 5).
T4606 799-887 Sentence denotes A similar phosphorylation was observed in Ramos cells, but not in HL60 cells (Figure 6).
T68 799-887 Sentence denotes A similar phosphorylation was observed in Ramos cells, but not in HL60 cells (Figure 6).
T4607 888-1021 Sentence denotes Furthermore, we also tested whether other known downstream signalling pathways of BMP-6 could be triggered by BMP-6 in human B cells.
T69 888-1021 Sentence denotes Furthermore, we also tested whether other known downstream signalling pathways of BMP-6 could be triggered by BMP-6 in human B cells.
T4608 1022-1168 Sentence denotes However, we did not observe any significant changes in the level of phospho-STAT3 or phospho-p38 upon BMP-6 treatment of B cells (data not shown).
T70 1022-1168 Sentence denotes However, we did not observe any significant changes in the level of phospho-STAT3 or phospho-p38 upon BMP-6 treatment of B cells (data not shown).