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PMC:1064895 / 9463-10900 JSONTXT 24 Projects

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Id Subject Object Predicate Lexical cue
T7493 0-118 Sentence denotes IL-17 in culture supernatants was measured by sandwich enzyme-linked immunosorbent assay as described previously [20].
T73 0-118 Sentence denotes IL-17 in culture supernatants was measured by sandwich enzyme-linked immunosorbent assay as described previously [20].
T7494 119-245 Sentence denotes In brief, a 96-well plate (Nunc) was coated with 4 μg/ml monoclonal antibodies against IL-17 (R & D Systems) at 4°C overnight.
T74 119-245 Sentence denotes In brief, a 96-well plate (Nunc) was coated with 4 μg/ml monoclonal antibodies against IL-17 (R & D Systems) at 4°C overnight.
T7495 246-522 Sentence denotes After blocking with phosphate-buffered saline/1% bovine serum albumin (BSA)/0.05% Tween 20 for 2 hours at room temperature (22–25°C), test samples and the standard recombinant IL-17 (R & D Systems) were added to the 96-well plate and incubated at room temperature for 2 hours.
T75 246-522 Sentence denotes After blocking with phosphate-buffered saline/1% bovine serum albumin (BSA)/0.05% Tween 20 for 2 hours at room temperature (22–25°C), test samples and the standard recombinant IL-17 (R & D Systems) were added to the 96-well plate and incubated at room temperature for 2 hours.
T7496 523-731 Sentence denotes Plates were washed four times with phosphate-buffered saline/Tween 20, and then incubated with 500 ng/ml biotinylated mouse monoclonal antibodies against IL-17 (R & D Systems) for 2 hours at room temperature.
T76 523-731 Sentence denotes Plates were washed four times with phosphate-buffered saline/Tween 20, and then incubated with 500 ng/ml biotinylated mouse monoclonal antibodies against IL-17 (R & D Systems) for 2 hours at room temperature.
T7497 732-990 Sentence denotes After washing, streptavidin–alkaline phosphate–horseradish peroxidase conjugate (Sigma) was incubated for 2 hours, then washed again and incubated with 1 mg/ml p-nitrophenyl phosphate (Sigma) dissolved in diethanolamine (Sigma) to develop the color reaction.
T77 732-990 Sentence denotes After washing, streptavidin–alkaline phosphate–horseradish peroxidase conjugate (Sigma) was incubated for 2 hours, then washed again and incubated with 1 mg/ml p-nitrophenyl phosphate (Sigma) dissolved in diethanolamine (Sigma) to develop the color reaction.
T7498 991-1100 Sentence denotes The reaction was stopped by the addition of 1 M NaOH and the optical density of each well was read at 405 nm.
T78 991-1100 Sentence denotes The reaction was stopped by the addition of 1 M NaOH and the optical density of each well was read at 405 nm.
T7499 1101-1149 Sentence denotes The lower limit of IL-17 detection was 10 pg/ml.
T79 1101-1149 Sentence denotes The lower limit of IL-17 detection was 10 pg/ml.
T7500 1150-1266 Sentence denotes Recombinant human IL-17 diluted in culture medium was used as a calibration standard, ranging from 10 to 2000 pg/ml.
T80 1150-1266 Sentence denotes Recombinant human IL-17 diluted in culture medium was used as a calibration standard, ranging from 10 to 2000 pg/ml.
T7501 1267-1437 Sentence denotes A standard curve was drawn by plotting optical density against the log of the concentration of recombinant cytokines, and used for determination of IL-17 in test samples.
T81 1267-1437 Sentence denotes A standard curve was drawn by plotting optical density against the log of the concentration of recombinant cytokines, and used for determination of IL-17 in test samples.