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PMC:1064895 / 5649-15482 JSONTXT 26 Projects

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Id Subject Object Predicate Lexical cue
T36 0-21 Sentence denotes Materials and methods
T4872 23-31 Sentence denotes Patients
T37 23-31 Sentence denotes Patients
T4873 32-240 Sentence denotes Informed consent was obtained from 24 patients (5 men and 19 women) with RA who fulfilled the 1987 revised criteria of the American College of Rheumatology (formerly the American Rheumatism Association) [17].
T38 32-240 Sentence denotes Informed consent was obtained from 24 patients (5 men and 19 women) with RA who fulfilled the 1987 revised criteria of the American College of Rheumatology (formerly the American Rheumatism Association) [17].
T4874 241-323 Sentence denotes The age of the patients with RA was 50 ± 8 (mean ± SEM) years (range 23–71 years).
T39 241-323 Sentence denotes The age of the patients with RA was 50 ± 8 (mean ± SEM) years (range 23–71 years).
T4875 324-388 Sentence denotes All medications were stopped 48 hours before entry to the study.
T40 324-388 Sentence denotes All medications were stopped 48 hours before entry to the study.
T4876 389-537 Sentence denotes Comparisons were made with 14 patients with OA (3 men and 11 women) and with 14 healthy controls (3 men and 11 women) who had no rheumatic diseases.
T41 389-537 Sentence denotes Comparisons were made with 14 patients with OA (3 men and 11 women) and with 14 healthy controls (3 men and 11 women) who had no rheumatic diseases.
T4877 538-676 Sentence denotes The mean ages of the patients with OA and the healthy controls were 50 ± 8 years (range 34–68 years) and 30 ± 6 years (range 24–57 years).
T42 538-676 Sentence denotes The mean ages of the patients with OA and the healthy controls were 50 ± 8 years (range 34–68 years) and 30 ± 6 years (range 24–57 years).
T4878 677-806 Sentence denotes Informed consent was obtained, and the protocol was approved by the Catholic University of Korea Human Research Ethics Committee.
T43 677-806 Sentence denotes Informed consent was obtained, and the protocol was approved by the Catholic University of Korea Human Research Ethics Committee.
T5348 808-816 Sentence denotes Reagents
T44 808-816 Sentence denotes Reagents
T5349 817-1015 Sentence denotes Recombinant IL-17, IL-18, IL-15, monocyte chemoattractant protein-1 (MCP-1), macrophage inflammatory protein (MIP)-1α, MIP-1β, IL-6 and IL-8 were purchased from R & D systems (Minneapolis, MN, USA).
T45 817-1015 Sentence denotes Recombinant IL-17, IL-18, IL-15, monocyte chemoattractant protein-1 (MCP-1), macrophage inflammatory protein (MIP)-1α, MIP-1β, IL-6 and IL-8 were purchased from R & D systems (Minneapolis, MN, USA).
T5350 1016-1105 Sentence denotes Recombinant transforming growth factor (TGF)-β was purchased from Peprotech (London, UK).
T46 1016-1105 Sentence denotes Recombinant transforming growth factor (TGF)-β was purchased from Peprotech (London, UK).
T5351 1106-1187 Sentence denotes Recombinant TNF-α and IL-1 were purchased from Endogen Inc. (Cambridge, MA, USA).
T47 1106-1187 Sentence denotes Recombinant TNF-α and IL-1 were purchased from Endogen Inc. (Cambridge, MA, USA).
T5352 1188-1251 Sentence denotes Cyclosporin A was provided by Sandos Ltd. (Basel, Switzerland).
T48 1188-1251 Sentence denotes Cyclosporin A was provided by Sandos Ltd. (Basel, Switzerland).
T5353 1252-1418 Sentence denotes Phytohemagglutinin (PHA), pyrrolidine dithiocarbamate (PDTC), rapamycin, dexamethasone and curcumin were all obtained from the Sigma Chemical Co. (St Louis, MA, USA).
T49 1252-1418 Sentence denotes Phytohemagglutinin (PHA), pyrrolidine dithiocarbamate (PDTC), rapamycin, dexamethasone and curcumin were all obtained from the Sigma Chemical Co. (St Louis, MA, USA).
T5354 1419-1537 Sentence denotes Anti-CD3 monoclonal antibody and anti-CD28 monoclonal antibody were obtained from BD Biosciences (San Diego, CA, USA).
T50 1419-1537 Sentence denotes Anti-CD3 monoclonal antibody and anti-CD28 monoclonal antibody were obtained from BD Biosciences (San Diego, CA, USA).
T5355 1538-1640 Sentence denotes LY294002, SB203580, FK506, wortmannin and PD98059 were obtained from Calbiochem (Schwalbach, Germany).
T51 1538-1640 Sentence denotes LY294002, SB203580, FK506, wortmannin and PD98059 were obtained from Calbiochem (Schwalbach, Germany).
T6081 1642-1716 Sentence denotes Production of IL-17 by T cell receptor activation, cytokines or chemokines
T52 1642-1716 Sentence denotes Production of IL-17 by T cell receptor activation, cytokines or chemokines
T6082 1717-1818 Sentence denotes PBMC were prepared from heparinized blood by Ficoll-Hypaque (SG1077) density-gradient centrifugation.
T53 1717-1818 Sentence denotes PBMC were prepared from heparinized blood by Ficoll-Hypaque (SG1077) density-gradient centrifugation.
T6083 1819-1877 Sentence denotes Cell cultures were performed as described previously [18].
T54 1819-1877 Sentence denotes Cell cultures were performed as described previously [18].
T6084 1878-2077 Sentence denotes In brief, the cell suspensions were adjusted to a concentration of 106/ml in RPMI 1640 medium supplemented with 10% fetal calf serum, 100 U/ml penicillin, 100 mg/ml streptomycin and 2 mM L-glutamine.
T55 1878-2077 Sentence denotes In brief, the cell suspensions were adjusted to a concentration of 106/ml in RPMI 1640 medium supplemented with 10% fetal calf serum, 100 U/ml penicillin, 100 mg/ml streptomycin and 2 mM L-glutamine.
T6085 2078-2218 Sentence denotes Cell suspension (1 ml) was dispensed into 24-well multi-well plates (Nunc, Roskilde, Denmark), and incubated for 24 hours at 37°C in 5% CO2.
T56 2078-2218 Sentence denotes Cell suspension (1 ml) was dispensed into 24-well multi-well plates (Nunc, Roskilde, Denmark), and incubated for 24 hours at 37°C in 5% CO2.
T6086 2219-2351 Sentence denotes Subsequently, various concentrations of cyclosporin A (10–500 ng/ml) were added to the medium and cells were incubated for 24 hours.
T57 2219-2351 Sentence denotes Subsequently, various concentrations of cyclosporin A (10–500 ng/ml) were added to the medium and cells were incubated for 24 hours.
T6087 2352-2466 Sentence denotes To each well was added FK506, rapamycin, curcumin, PDTC, LY294002, SB203580, PD98059, dexamethasone or wortmannin.
T58 2352-2466 Sentence denotes To each well was added FK506, rapamycin, curcumin, PDTC, LY294002, SB203580, PD98059, dexamethasone or wortmannin.
T6088 2467-2589 Sentence denotes After incubation for 24 hours (unless otherwise stated), cell-free media were collected and stored at -20°C until assayed.
T59 2467-2589 Sentence denotes After incubation for 24 hours (unless otherwise stated), cell-free media were collected and stored at -20°C until assayed.
T6089 2590-2671 Sentence denotes All cultures were set up in triplicate, and results are expressed as means ± SEM.
T60 2590-2671 Sentence denotes All cultures were set up in triplicate, and results are expressed as means ± SEM.
T6724 2673-2702 Sentence denotes CD4+ T-cell isolation by MACS
T61 2673-2702 Sentence denotes CD4+ T-cell isolation by MACS
T6725 2703-2796 Sentence denotes Anti-CD4 microbeads were used essentially as recommended by the manufacturer (Miltenyi) [19].
T62 2703-2796 Sentence denotes Anti-CD4 microbeads were used essentially as recommended by the manufacturer (Miltenyi) [19].
T6726 2797-2851 Sentence denotes PBMC were resuspended in 80 μl of FBS staining buffer.
T63 2797-2851 Sentence denotes PBMC were resuspended in 80 μl of FBS staining buffer.
T64 2852-2926 Sentence denotes Anti-CD4 microbeads (20 μl) were added and incubated for 15 min at 6–12°C.
T6727 2852-3016 Sentence denotes Anti-CD4 microbeads (20 μl) were added and incubated for 15 min at 6–12°C. Saturating amounts of fluorochrome-conjugated antibodies were added for a further 10 min.
T65 2927-3016 Sentence denotes Saturating amounts of fluorochrome-conjugated antibodies were added for a further 10 min.
T6728 3017-3189 Sentence denotes Cells were diluted in 2.5 ml of FBS staining buffer, pelleted, resuspended in 500 μl and magnetically separated, usually on an AutoMACS magnet fitted with a MACS MS column.
T66 3017-3189 Sentence denotes Cells were diluted in 2.5 ml of FBS staining buffer, pelleted, resuspended in 500 μl and magnetically separated, usually on an AutoMACS magnet fitted with a MACS MS column.
T6729 3190-3263 Sentence denotes Flow-through and two 1 ml washes were collected as the negative fraction.
T67 3190-3263 Sentence denotes Flow-through and two 1 ml washes were collected as the negative fraction.
T6730 3264-3363 Sentence denotes Enriched cells were collected in two 0.5 ml aliquots from the column after removal from the magnet.
T68 3264-3363 Sentence denotes Enriched cells were collected in two 0.5 ml aliquots from the column after removal from the magnet.
T6731 3364-3595 Sentence denotes Alternatively, cells stained with anti-CD4–phycoerythrin were washed, magnetically labeled with anti-phycoerythrin microbeads (20 μl added to 80 μl of cell suspension; 15 min, 6–12°C), and magnetically separated as described above.
T69 3364-3595 Sentence denotes Alternatively, cells stained with anti-CD4–phycoerythrin were washed, magnetically labeled with anti-phycoerythrin microbeads (20 μl added to 80 μl of cell suspension; 15 min, 6–12°C), and magnetically separated as described above.
T6732 3596-3699 Sentence denotes The purity of cells was assessed by flow cytometric analysis of stained cells on a FACS Vantage sorter.
T70 3596-3699 Sentence denotes The purity of cells was assessed by flow cytometric analysis of stained cells on a FACS Vantage sorter.
T6733 3700-3769 Sentence denotes Most (more than 97%) of the isolated cells had the CD4 T cell marker.
T71 3700-3769 Sentence denotes Most (more than 97%) of the isolated cells had the CD4 T cell marker.
T7492 3771-3813 Sentence denotes Enzyme-linked immunosorbent assay of IL-17
T72 3771-3813 Sentence denotes Enzyme-linked immunosorbent assay of IL-17
T7493 3814-3932 Sentence denotes IL-17 in culture supernatants was measured by sandwich enzyme-linked immunosorbent assay as described previously [20].
T73 3814-3932 Sentence denotes IL-17 in culture supernatants was measured by sandwich enzyme-linked immunosorbent assay as described previously [20].
T7494 3933-4059 Sentence denotes In brief, a 96-well plate (Nunc) was coated with 4 μg/ml monoclonal antibodies against IL-17 (R & D Systems) at 4°C overnight.
T74 3933-4059 Sentence denotes In brief, a 96-well plate (Nunc) was coated with 4 μg/ml monoclonal antibodies against IL-17 (R & D Systems) at 4°C overnight.
T7495 4060-4336 Sentence denotes After blocking with phosphate-buffered saline/1% bovine serum albumin (BSA)/0.05% Tween 20 for 2 hours at room temperature (22–25°C), test samples and the standard recombinant IL-17 (R & D Systems) were added to the 96-well plate and incubated at room temperature for 2 hours.
T75 4060-4336 Sentence denotes After blocking with phosphate-buffered saline/1% bovine serum albumin (BSA)/0.05% Tween 20 for 2 hours at room temperature (22–25°C), test samples and the standard recombinant IL-17 (R & D Systems) were added to the 96-well plate and incubated at room temperature for 2 hours.
T7496 4337-4545 Sentence denotes Plates were washed four times with phosphate-buffered saline/Tween 20, and then incubated with 500 ng/ml biotinylated mouse monoclonal antibodies against IL-17 (R & D Systems) for 2 hours at room temperature.
T76 4337-4545 Sentence denotes Plates were washed four times with phosphate-buffered saline/Tween 20, and then incubated with 500 ng/ml biotinylated mouse monoclonal antibodies against IL-17 (R & D Systems) for 2 hours at room temperature.
T7497 4546-4804 Sentence denotes After washing, streptavidin–alkaline phosphate–horseradish peroxidase conjugate (Sigma) was incubated for 2 hours, then washed again and incubated with 1 mg/ml p-nitrophenyl phosphate (Sigma) dissolved in diethanolamine (Sigma) to develop the color reaction.
T77 4546-4804 Sentence denotes After washing, streptavidin–alkaline phosphate–horseradish peroxidase conjugate (Sigma) was incubated for 2 hours, then washed again and incubated with 1 mg/ml p-nitrophenyl phosphate (Sigma) dissolved in diethanolamine (Sigma) to develop the color reaction.
T7498 4805-4914 Sentence denotes The reaction was stopped by the addition of 1 M NaOH and the optical density of each well was read at 405 nm.
T78 4805-4914 Sentence denotes The reaction was stopped by the addition of 1 M NaOH and the optical density of each well was read at 405 nm.
T7499 4915-4963 Sentence denotes The lower limit of IL-17 detection was 10 pg/ml.
T79 4915-4963 Sentence denotes The lower limit of IL-17 detection was 10 pg/ml.
T7500 4964-5080 Sentence denotes Recombinant human IL-17 diluted in culture medium was used as a calibration standard, ranging from 10 to 2000 pg/ml.
T80 4964-5080 Sentence denotes Recombinant human IL-17 diluted in culture medium was used as a calibration standard, ranging from 10 to 2000 pg/ml.
T7501 5081-5251 Sentence denotes A standard curve was drawn by plotting optical density against the log of the concentration of recombinant cytokines, and used for determination of IL-17 in test samples.
T81 5081-5251 Sentence denotes A standard curve was drawn by plotting optical density against the log of the concentration of recombinant cytokines, and used for determination of IL-17 in test samples.
T8623 5253-5349 Sentence denotes Quantification of IL-17 mRNA by semiquantitative reverse transcription–polymerase chain reaction
T82 5253-5349 Sentence denotes Quantification of IL-17 mRNA by semiquantitative reverse transcription–polymerase chain reaction
T8624 5350-5468 Sentence denotes PBMC were incubated with various concentrations of anti-CD3 in the presence or absence of inhibitors (LY294002, PDTC).
T83 5350-5468 Sentence denotes PBMC were incubated with various concentrations of anti-CD3 in the presence or absence of inhibitors (LY294002, PDTC).
T8625 5469-5623 Sentence denotes After 16 hours of incubation, mRNA was extracted with RNAzol B (Biotex Laboratories, Houston, TX, USA) in accordance with the manufacturer's instructions.
T84 5469-5623 Sentence denotes After 16 hours of incubation, mRNA was extracted with RNAzol B (Biotex Laboratories, Houston, TX, USA) in accordance with the manufacturer's instructions.
T8626 5624-5765 Sentence denotes Reverse transcription of 2 μg of total mRNA was performed at 42°C using the Superscript™ reverse transcription system (Takara, Shiga, Japan).
T85 5624-5765 Sentence denotes Reverse transcription of 2 μg of total mRNA was performed at 42°C using the Superscript™ reverse transcription system (Takara, Shiga, Japan).
T8627 5766-5919 Sentence denotes PCR amplification of cDNA aliquots was performed by adding 2.5 mM dNTPs, 2.5 U of Taq DNA polymerase (Takara) and 0.25 μM of sense and antisense primers.
T86 5766-5919 Sentence denotes PCR amplification of cDNA aliquots was performed by adding 2.5 mM dNTPs, 2.5 U of Taq DNA polymerase (Takara) and 0.25 μM of sense and antisense primers.
T8628 5920-6038 Sentence denotes The reaction was performed in PCR buffer (1.5 mM MgCl2, 50 mM KCl, 10 mM Tris-HCl, pH 8.3) in a total volume of 25 μl.
T87 5920-6038 Sentence denotes The reaction was performed in PCR buffer (1.5 mM MgCl2, 50 mM KCl, 10 mM Tris-HCl, pH 8.3) in a total volume of 25 μl.
T8629 6039-6358 Sentence denotes The following sense and antisense primers for each molecules were used: IL-17 sense, 5'-ATG ACT CCT GGG AAG ACC TCA TTG-3'; IL-17 antisense, 5'-TTA GGC CAC ATG GTG GAC AAT CGG-3'; glyceraldehyde-3-phosphate dehydrogenase (GAPDH) sense, 5'-CGA TGC TGG GCG TGA GTA C-3'; GAPDH antisense, 5'-CGT TCA GCT CAG GGA TGA CC-3'.
T88 6039-6358 Sentence denotes The following sense and antisense primers for each molecules were used: IL-17 sense, 5'-ATG ACT CCT GGG AAG ACC TCA TTG-3'; IL-17 antisense, 5'-TTA GGC CAC ATG GTG GAC AAT CGG-3'; glyceraldehyde-3-phosphate dehydrogenase (GAPDH) sense, 5'-CGA TGC TGG GCG TGA GTA C-3'; GAPDH antisense, 5'-CGT TCA GCT CAG GGA TGA CC-3'.
T89 6359-6584 Sentence denotes Reactions were processed in a DNA thermal cycler (Perkin-Elmer Cetus, Norwalk, CT, USA) through cycles for 30 s of denaturation at 94°C, 1 min of annealing at 56°C for GAPDH and IL-17, followed by 1 min of elongation at 72°C.
T8630 6359-6750 Sentence denotes Reactions were processed in a DNA thermal cycler (Perkin-Elmer Cetus, Norwalk, CT, USA) through cycles for 30 s of denaturation at 94°C, 1 min of annealing at 56°C for GAPDH and IL-17, followed by 1 min of elongation at 72°C. PCR rounds were repeated for 25 cycles each for both GAPDH and IL-17; this was determined as falling within the exponential phase of amplification for each molecule.
T90 6585-6750 Sentence denotes PCR rounds were repeated for 25 cycles each for both GAPDH and IL-17; this was determined as falling within the exponential phase of amplification for each molecule.
T8631 6751-6845 Sentence denotes The level of mRNA expression was presented as a ratio of IL-17 PCR product over GAPDH product.
T91 6751-6845 Sentence denotes The level of mRNA expression was presented as a ratio of IL-17 PCR product over GAPDH product.
T9362 6847-6905 Sentence denotes Western blot analysis of Akt, phosphorylated Akt and IκB-α
T92 6847-6905 Sentence denotes Western blot analysis of Akt, phosphorylated Akt and IκB-α
T9363 6906-6998 Sentence denotes PBMC were incubated with anti-CD3 (10 μg/ml) in the presence or absence of LY294002 (20 μM).
T93 6906-6998 Sentence denotes PBMC were incubated with anti-CD3 (10 μg/ml) in the presence or absence of LY294002 (20 μM).
T9364 6999-7176 Sentence denotes After incubation for 1 hour, whole cell lysates were prepared from about 107 cells by homogenization in the lysis buffer, and centrifuged at 14,000 r.p.m. (19,000 g) for 15 min.
T94 6999-7176 Sentence denotes After incubation for 1 hour, whole cell lysates were prepared from about 107 cells by homogenization in the lysis buffer, and centrifuged at 14,000 r.p.m. (19,000 g) for 15 min.
T9365 7177-7290 Sentence denotes Protein concentrations in the supernatants were determined with the Bradford method (Bio-Rad, Hercules, CA, USA).
T95 7177-7290 Sentence denotes Protein concentrations in the supernatants were determined with the Bradford method (Bio-Rad, Hercules, CA, USA).
T9366 7291-7429 Sentence denotes Protein samples were separated by 10% SDS–PAGE and transferred to a nitrocellulose membrane (Amersham Pharmacia Biotech, Uppsala, Sweden).
T96 7291-7429 Sentence denotes Protein samples were separated by 10% SDS–PAGE and transferred to a nitrocellulose membrane (Amersham Pharmacia Biotech, Uppsala, Sweden).
T97 7430-7787 Sentence denotes For western hybridization, membrane was preincubated with 0.1% skimmed milk in TBS-T buffer (0.1% Tween 20 in Tris-buffered saline) at room temperature for 2 hours, then primary antibodies against Akt, phosphorylated Akt and IκB-α (Cell Signaling Technology Inc., Beverly, MA, USA), diluted 1:1000 in 5% BSA/TBS-T, were added and incubated overnight at 4°C.
T9367 7430-7946 Sentence denotes For western hybridization, membrane was preincubated with 0.1% skimmed milk in TBS-T buffer (0.1% Tween 20 in Tris-buffered saline) at room temperature for 2 hours, then primary antibodies against Akt, phosphorylated Akt and IκB-α (Cell Signaling Technology Inc., Beverly, MA, USA), diluted 1:1000 in 5% BSA/TBS-T, were added and incubated overnight at 4°C. After washing four times with TBS-T, horseradish peroxidase-conjugated secondary antibodies were added and allowed to incubate for 1 hour at room temperature.
T98 7788-7946 Sentence denotes After washing four times with TBS-T, horseradish peroxidase-conjugated secondary antibodies were added and allowed to incubate for 1 hour at room temperature.
T9368 7947-8103 Sentence denotes After TBS-T washing, hybridized bands were detected with the enhanced chemiluminescence (ECL) detection kit and Hyperfilm-ECL reagents (Amersham Pharmacia).
T99 7947-8103 Sentence denotes After TBS-T washing, hybridized bands were detected with the enhanced chemiluminescence (ECL) detection kit and Hyperfilm-ECL reagents (Amersham Pharmacia).
T10177 8105-8151 Sentence denotes Gel mobility-shift assay of NF-κB binding site
T100 8105-8151 Sentence denotes Gel mobility-shift assay of NF-κB binding site
T10178 8152-8208 Sentence denotes Nuclear proteins were extracted from about 5 × 106 PBMC.
T101 8152-8208 Sentence denotes Nuclear proteins were extracted from about 5 × 106 PBMC.
T10179 8209-8466 Sentence denotes Oligonucleotide probes encompassing the NF-κB binding site of the human IL-17 promoter (5'-ATG ACC TGG AAA TAC CCA AAA TTC-3') were generated by 5'-end labeling of the sense strand with [γ-32P]dATP (Amersham Pharmacia) and T4 polynucleotide kinase (TaKaRa).
T102 8209-8466 Sentence denotes Oligonucleotide probes encompassing the NF-κB binding site of the human IL-17 promoter (5'-ATG ACC TGG AAA TAC CCA AAA TTC-3') were generated by 5'-end labeling of the sense strand with [γ-32P]dATP (Amersham Pharmacia) and T4 polynucleotide kinase (TaKaRa).
T10180 8467-8577 Sentence denotes Unincorporated nucleotides were removed by NucTrap probe purification columns (Stratagene, La Jolla, CA, USA).
T103 8467-8577 Sentence denotes Unincorporated nucleotides were removed by NucTrap probe purification columns (Stratagene, La Jolla, CA, USA).
T10181 8578-8890 Sentence denotes Nuclear extracts (2 μg of protein) were incubated with radiolabeled DNA probes (10 ng; 100,000 c.p.m.) for 30 min at room temperature in 20 μl of binding buffer consisting of 20 mM Tris-HCl, pH 7.9, 50 mM KCl, 1 mM dithiothreitol, 0.5 mM EDTA, 5% glycerol, 1 mg/ml BSA, 0.2% Nonidet P40 and 50 ng/μl poly(dI-dC).
T104 8578-8890 Sentence denotes Nuclear extracts (2 μg of protein) were incubated with radiolabeled DNA probes (10 ng; 100,000 c.p.m.) for 30 min at room temperature in 20 μl of binding buffer consisting of 20 mM Tris-HCl, pH 7.9, 50 mM KCl, 1 mM dithiothreitol, 0.5 mM EDTA, 5% glycerol, 1 mg/ml BSA, 0.2% Nonidet P40 and 50 ng/μl poly(dI-dC).
T105 8891-9024 Sentence denotes Samples were subjected to electrophoresis on nondenaturing 5% polyacrylamide gels in 0.5 × Tris-borate-EDTA buffer (pH 8.0) at 100 V.
T10182 8891-9122 Sentence denotes Samples were subjected to electrophoresis on nondenaturing 5% polyacrylamide gels in 0.5 × Tris-borate-EDTA buffer (pH 8.0) at 100 V. Gels were dried under vacuum and exposed to Kodak X-OMAT film at -70°C with intensifying screens.
T106 9025-9122 Sentence denotes Gels were dried under vacuum and exposed to Kodak X-OMAT film at -70°C with intensifying screens.
T10183 9123-9251 Sentence denotes Rabbit polyclonal antibodies against NF-κB subunits p50, p65 and c-Rel were from Santa Cruz Biotechnology (Santa Cruz, CA, USA).
T107 9123-9251 Sentence denotes Rabbit polyclonal antibodies against NF-κB subunits p50, p65 and c-Rel were from Santa Cruz Biotechnology (Santa Cruz, CA, USA).
T10822 9253-9301 Sentence denotes Cell viability (Trypan blue dye exclusion assay)
T108 9253-9301 Sentence denotes Cell viability (Trypan blue dye exclusion assay)
T10823 9302-9451 Sentence denotes For cell viability assays, the trypan blue dye exclusion method was used to evaluate the potential of direct cytotoxic effect of inhibitors on cells.
T109 9302-9451 Sentence denotes For cell viability assays, the trypan blue dye exclusion method was used to evaluate the potential of direct cytotoxic effect of inhibitors on cells.
T10824 9452-9647 Sentence denotes After incubation for 24 hours, the cells were harvested and the percentage cell viability was calculated with the formula 100 × (number of viable cells/number of both viable and dead cells) [21].
T110 9452-9647 Sentence denotes After incubation for 24 hours, the cells were harvested and the percentage cell viability was calculated with the formula 100 × (number of viable cells/number of both viable and dead cells) [21].
T11014 9649-9669 Sentence denotes Statistical analysis
T111 9649-9669 Sentence denotes Statistical analysis
T11015 9670-9704 Sentence denotes Data are expressed as means ± SEM.
T112 9670-9704 Sentence denotes Data are expressed as means ± SEM.
T11016 9705-9780 Sentence denotes Statistical analysis was performed with Student's t-test for matched pairs.
T113 9705-9780 Sentence denotes Statistical analysis was performed with Student's t-test for matched pairs.
T11017 9781-9833 Sentence denotes P values less than 0.05 were considered significant.
T114 9781-9833 Sentence denotes P values less than 0.05 were considered significant.