| Id |
Subject |
Object |
Predicate |
Lexical cue |
| S1 |
0-100 |
Sentence |
denotes |
ALY, a context-dependent coactivator of LEF-1 and AML-1, is required for TCRalpha enhancer function. |
| S2 |
101-311 |
Sentence |
denotes |
LEF-1 is a transcription factor that participates in the regulation of the T-cell receptor alpha (TCR alpha) enhancer by facilitating the assembly of multiple proteins into a higher order nucleoprotein complex. |
| S3 |
312-539 |
Sentence |
denotes |
The function of LEF-1 is dependent, in part, on the HMG domain that induces a sharp bend in the DNA helix, and on an activation domain that stimulates transcription only in a specific context of other enhancer-binding proteins. |
| S4 |
540-680 |
Sentence |
denotes |
With the aim of gaining insight into the function of context-dependent activation domains, we cloned ALY, a novel LEF-1-interacting protein. |
| S5 |
681-904 |
Sentence |
denotes |
ALY is a ubiquitously expressed, nuclear protein that specifically associates with the activation domains of LEF-1 and AML-1 (CBF alpha2, PEBP2 alpha(B), which is another protein component of the TCR alpha enhancer complex. |
| S6 |
905-978 |
Sentence |
denotes |
In addition, ALY can increase DNA binding by both LEF-1 and AML proteins. |
| S7 |
979-1234 |
Sentence |
denotes |
Overexpression of ALY stimulates the activity of the TCR alpha enhancer complex reconstituted in transfected nonlymphoid HeLa cells, whereas down-regulation of ALY by anti-sense oligonucleotides virtually eliminates TCR alpha enhancer activity in T cells. |
| S8 |
1235-1409 |
Sentence |
denotes |
Similar to LEF-1, ALY can stimulate transcription in the context of the TCR alpha enhancer but apparently not when tethered to DNA through an heterologous DNA-binding domain. |
| S9 |
1410-1584 |
Sentence |
denotes |
We propose that ALY mediates context-dependent transcriptional activation by facilitating the functional collaboration of multiple proteins in the TCR alpha enhancer complex. |
