| Id |
Subject |
Object |
Predicate |
Lexical cue |
| S1 |
0-161 |
Sentence |
denotes |
Negative transcriptional regulation of human interleukin 2 (IL-2) gene by glucocorticoids through interference with nuclear transcription factors AP-1 and NF-AT. |
| S2 |
162-226 |
Sentence |
denotes |
IL-2 gene transcription is affected by several nuclear proteins. |
| S3 |
227-404 |
Sentence |
denotes |
We asked whether dexamethasone (Dex) and cyclosporin A (CsA) inhibit IL-2 gene transcription by interfering with the activity of nuclear proteins that bind to the IL-2 promoter. |
| S4 |
405-514 |
Sentence |
denotes |
Nuclear extracts from primary human T lymphocytes were analyzed by electrophoretic DNA mobility shift assays. |
| S5 |
515-682 |
Sentence |
denotes |
Both Dex and CsA inhibited the binding of transcription factors AP-1 and NF-AT, but not of NF-kB and OCT-1/OAF, to their corresponding sites on the IL-2 gene promoter. |
| S6 |
683-882 |
Sentence |
denotes |
To correlate changes in nuclear factor binding in vitro with transcriptional activity in vivo and define the structural requirements for IL-2 promoter repression, we used transient DNA transfections. |
| S7 |
883-1007 |
Sentence |
denotes |
Jurkat cells were transfected with plasmids containing either the intact IL-2 promoter or its AP-1, NF-AT, and NF-kB motifs. |
| S8 |
1008-1091 |
Sentence |
denotes |
Dex inhibited the IL-2 promoter and the AP-1, but not the NF-AT and NF-kB plasmids. |
| S9 |
1092-1188 |
Sentence |
denotes |
In contrast, CsA inhibited the IL-2 promoter and the NF-AT, but not the AP-1 and NF-kB plasmids. |
| S10 |
1189-1353 |
Sentence |
denotes |
These results suggest that in human T lymphocytes both Dex and CsA inhibited IL-2 gene transcription through interference with transcription factors AP-1 and NF-AT. |
| S11 |
1354-1487 |
Sentence |
denotes |
We propose that, while maximum inhibition may involve interaction with both transcription factors, AP-1 is the primary target of Dex. |
