| Id |
Subject |
Object |
Predicate |
Lexical cue |
| S1 |
0-127 |
Sentence |
denotes |
In vivo modification of major histocompatibility complex class II DRA promoter occupancy mediated by the AIR-1 trans-activator. |
| S2 |
128-253 |
Sentence |
denotes |
RJ 2.2.5 is a human B cell mutant derived from the Burkitt lymphoma Raji cell which is defective in the AIR-1 locus function. |
| S3 |
254-405 |
Sentence |
denotes |
This locus encodes a transcriptional trans-activator required for the constitutive expression of major histocompatibility complex (MHC) class II genes. |
| S4 |
406-535 |
Sentence |
denotes |
Here we show, by in vivo DNase I footprinting, that the AIR-1 locus defect correlates with changes in the DRA promoter occupancy. |
| S5 |
536-715 |
Sentence |
denotes |
Interestingly, reexpression of human MHC class II genes in RJ 2.2.5 x mouse spleen cell hybrids is associated with partial reversion of DRA promoter occupancy to the Raji pattern. |
| S6 |
716-907 |
Sentence |
denotes |
DRA promoter occupancy in other class II-negative B cell lines, derived from patients with bare lymphocyte syndrome, is drastically different from the one observed in RJ 2.2.5 and Raji cells. |
| S7 |
908-1128 |
Sentence |
denotes |
Moreover, the use of the DNase I as an in vivo footprinting agent reveals that the patients' cell lines do not display a completely "bare promoter" as previously reported using dimethyl sulfate as the footprinting agent. |
| S8 |
1129-1403 |
Sentence |
denotes |
Thus, the use of DNase I allowed us, for the first time, to correlate the AIR-1 locus defect with class II promoter occupancy alterations and distinguish these alterations from the ones observed in phenotypically similar but genetically distinct MHC class II-negative cells. |
