| Id |
Subject |
Object |
Predicate |
Lexical cue |
| S1 |
0-143 |
Sentence |
denotes |
Identification of human TR2 orphan receptor response element in the transcriptional initiation site of the simian virus 40 major late promoter. |
| S2 |
144-391 |
Sentence |
denotes |
A DNA response element (TR2RE-SV40) for the TR2 orphan receptor, a member of the steroid-thyroid hormone receptor superfamily, has been identified in the simian virus 40 (SV40) +55 region (nucleotide numbers 368-389, 5'-GTTAAGGTTCGTAGGTCATGGA-3'). |
| S3 |
392-615 |
Sentence |
denotes |
Electrophoretic mobility shift assay, using in vitro translated TR2 orphan receptor with a molecular mass of 67 kilodaltons, showed a specific binding with high affinity (dissociation constant = 9 nM) for this DNA sequence. |
| S4 |
616-993 |
Sentence |
denotes |
DNA-swap experiments using chloramphenicol acetyl-transferase assay demonstrated that androgen can suppress the transcriptional activities of SV40 early promoter via the interaction between this TR2RE-SV40 and the chimeric receptor AR/TR2/AR with the DNA-binding domain of the TR2 orphan receptor flanked by the N-terminal and androgen-binding domains of the androgen receptor. |
| S5 |
994-1132 |
Sentence |
denotes |
In addition, this TR2RE-SV40 can function as a repressor to suppress the transcriptional activities of both SV40 early and late promoters. |
| S6 |
1133-1331 |
Sentence |
denotes |
Together, these data suggest the TR2RE-SV40 may represent the first identified natural DNA response element for the TR2 orphan receptor that may function as a repressor for the SV40 gene expression. |
