| Id |
Subject |
Object |
Predicate |
Lexical cue |
| S1 |
0-62 |
Sentence |
denotes |
T-cell functional regions of the human IL-3 proximal promoter. |
| S2 |
63-152 |
Sentence |
denotes |
The human interleukin-3 (IL-3) gene is expressed almost exclusively in activated T cells. |
| S3 |
153-240 |
Sentence |
denotes |
Its expression is regulated at both the transcriptional and post-transcriptional level. |
| S4 |
241-410 |
Sentence |
denotes |
We have previously shown that treatment of Jurkat T cells with phytohemaglutinin (PHA) and the phorbol ester, PMA, activated transcription initiation from the IL-3 gene. |
| S5 |
411-604 |
Sentence |
denotes |
To define the regions of the gene required for transcription activation, we generated a series of reporter constructs containing different regions of the IL-3 gene 5' and 3' flanking sequences. |
| S6 |
605-720 |
Sentence |
denotes |
Both positive and negative regulatory elements were identified in the proximal 5' flanking region of the IL-3 gene. |
| S7 |
721-806 |
Sentence |
denotes |
The promoter region between -173 and -60 contained the strongest activating elements. |
| S8 |
807-898 |
Sentence |
denotes |
The transcription factor AP-1 could bind to this positive activator region of the promoter. |
| S9 |
899-1150 |
Sentence |
denotes |
We also examined the function of the IL-3 CK-1/CK-2 elements that are present in many cytokine genes and found that they acted as a repressor of basal level expression when cloned upstream of a heterologous promoter but were also inducible by PMA/PHA. |
