| Id |
Subject |
Object |
Predicate |
Lexical cue |
| S1 |
0-156 |
Sentence |
denotes |
Latent membrane protein-1 induces cyclin D2 expression, pRb hyperphosphorylation, and loss of TGF-beta 1-mediated growth inhibition in EBV-positive B cells. |
| S2 |
157-323 |
Sentence |
denotes |
The normal cell cycle is regulated by several molecules, such as the tumor-suppressor protein pRb, the G1 cyclins, the cyclin-dependent kinases, and their inhibitors. |
| S3 |
324-427 |
Sentence |
denotes |
These regulators are targeted by negative growth regulatory signals, such as that provided by TGF-beta. |
| S4 |
428-616 |
Sentence |
denotes |
Here, we show that the presence of either wild-type EBV or its transforming latent membrane protein-1 (LMP-1) results in the loss of TGF-beta 1-mediated growth inhibition in human B cells. |
| S5 |
617-935 |
Sentence |
denotes |
Chemical cross-linking with 125I-labeled TGF-beta 1 showed an essentially normal TGF-beta receptor profile in EBV-positive and EBV-negative Burkitt's lymphoma cell lines, and these receptors were shown to be functional in transducing signals, as evidenced by the TGF-beta 1-mediated modulation of junB gene expression. |
| S6 |
936-1191 |
Sentence |
denotes |
However, TGF-beta 1 did not induce dephosphorylation of pRb in EBV (or LMP-1)-positive cells as opposed to EBV-negative cells, suggesting a dichotomy in the TGF-beta 1 signaling pathway leading to separable gene regulatory and growth inhibitory responses. |
| S7 |
1192-1346 |
Sentence |
denotes |
Furthermore, LMP-1 was found to induce the expression of cyclin D2; normal B cells or EBV-negative Burkitt's lymphoma cells do not express D-type cyclins. |
| S8 |
1347-1593 |
Sentence |
denotes |
Taken together, these data point to a potential mechanism underlying EBV-mediated B cell transformation whereby constitutive induction of key cell cycle regulators by LMP-1 can lead to pRb hyperphosphorylation and uncontrolled cell proliferation. |
