| Id |
Subject |
Object |
Predicate |
Lexical cue |
| S1 |
0-64 |
Sentence |
denotes |
Nuclear factor-IL6 activates the human IL-4 promoter in T cells. |
| S2 |
65-180 |
Sentence |
denotes |
Positive regulatory element I (PRE-I) is a strong enhancer element essential for expression of the human IL-4 gene. |
| S3 |
181-371 |
Sentence |
denotes |
To identify transcription factors binding to PRE-I, we screened a cDNA expression library from Jurkat T cells and isolated a cDNA encoding nuclear factor (NF)-IL6 (also known as C/EBP beta). |
| S4 |
372-474 |
Sentence |
denotes |
NF-IL6 mRNA was found in human Jurkat T cells and in the mouse Th2 clone D10, but not in Th1 clone 29. |
| S5 |
475-545 |
Sentence |
denotes |
rNF-IL6 expressed in bacteria was shown to specifically bind to PRE-I. |
| S6 |
546-629 |
Sentence |
denotes |
PRE-I forms multiple DNA-protein complexes with nuclear extracts from Jurkat cells. |
| S7 |
630-719 |
Sentence |
denotes |
Some of these complexes were demonstrated to contain NF-IL6 by using anti-C/EBP beta Abs. |
| S8 |
720-919 |
Sentence |
denotes |
Overexpression of NF-IL6 enhanced expression of the chloramphenicol acetyl transferase reporter gene linked to the PRE-I-thymidine kinase or the human IL-4 promoter more than 10-fold in Jurkat cells. |
| S9 |
920-1084 |
Sentence |
denotes |
Promoter deletion studies revealed two additional NF-IL6 binding sites located at positions -44 to -36 (C/EBP proximal) and -87 to -79 (C/EBP medial), respectively. |
| S10 |
1085-1201 |
Sentence |
denotes |
Our results demonstrate that NF-IL6 is involved in transcriptional activation of the human IL-4 promoter in T cells. |
