pubmed-sentences-benchmark  

PubMed:7565736 JSONTXT 8 Projects

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Id Subject Object Predicate Lexical cue
S1 0-125 Sentence denotes PU.1 (Spi-1) and C/EBP alpha regulate expression of the granulocyte-macrophage colony-stimulating factor receptor alpha gene.
S2 126-190 Sentence denotes Growth factor receptors play an important role in hematopoiesis.
S3 191-476 Sentence denotes In order to further understand the mechanisms directing the expression of these key regulators of hematopoiesis, we initiated a study investigating the transcription factors activating the expression of the granulocyte-macrophage colony-stimulating factor (GM-CSF) receptor alpha gene.
S4 477-715 Sentence denotes Here, we demonstrate that the human GM-CSF receptor alpha promoter directs reporter gene activity in a tissue-specific fashion in myelomonocytic cells, which correlates with its expression pattern as analyzed by reverse transcription PCR.
S5 716-877 Sentence denotes The GM-CSF receptor alpha promoter contains an important functional site between positions -53 and -41 as identified by deletion analysis of reporter constructs.
S6 878-972 Sentence denotes We show that the myeloid and B cell transcription factor PU.1 binds specifically to this site.
S7 973-1174 Sentence denotes Furthermore, we demonstrate that a CCAAT site located upstream of the PU.1 site between positions -70 and -54 is involved in positive-negative regulation of the GM-CSF receptor alpha promoter activity.
S8 1175-1299 Sentence denotes C/EBP alpha is the major CCAAT/enhancer-binding protein (C/EBP) form binding to this site in nuclear extracts of U937 cells.
S9 1300-1514 Sentence denotes Point mutations of either the PU.1 site or the C/EBP site that abolish the binding of the respective factors result in a significant decrease of GM-CSF receptor alpha promoter activity in myelomonocytic cells only.
S10 1515-1706 Sentence denotes Furthermore, we demonstrate that in myeloid and B cell extracts, PU.1 forms a novel, specific, more slowly migrating complex (PU-SF) when binding the GM-CSF receptor alpha promoter PU.1 site.
S11 1707-1806 Sentence denotes This is the first demonstration of a specific interaction with PU.1 on a myeloid PU.1 binding site.
S12 1807-2085 Sentence denotes The novel complex is distinct from that described previously as binding to B cell enhancer sites and can be formed by addition of PU.1 to extracts from certain nonmyeloid cell types which do not express PU.1, including T cells and epithelial cells, but not from erythroid cells.
S13 2086-2281 Sentence denotes Furthermore, we demonstrate that the PU-SF complex binds to PU.1 sites found on a number of myeloid promoters, and its formation requires an intact PU.1 site adjacent to a single-stranded region.
S14 2282-2369 Sentence denotes Expression of PU.1 in nonmyeloid cells can activate the GM-CSF receptor alpha promoter.
S15 2370-2639 Sentence denotes Deletion of the amino-terminal region of PU.1 results in a failure to form the PU-SF complex and in a concomitant loss of transactivation, suggesting that formation of the PU-SF complex is of functional importance for the activity of the GM-CSF receptor alpha promoter.
S16 2640-2752 Sentence denotes Finally, we demonstrate that C/EBP alpha can also active the GM-CSF receptor alpha promoter in nonmyeloid cells.
S17 2753-3015 Sentence denotes These results suggest that PU.1 and C/EBP alpha direct the cell-type-specific expression of GM-CSF receptor alpha, further establish the role of PU.1 as a key regulator of hematopoiesis, and point to C/EBP alpha as an additional important factor in this process.