| Id |
Subject |
Object |
Predicate |
Lexical cue |
| S1 |
0-63 |
Sentence |
denotes |
Nitric oxide decreases cytokine-induced endothelial activation. |
| S2 |
64-172 |
Sentence |
denotes |
Nitric oxide selectively reduces endothelial expression of adhesion molecules and proinflammatory cytokines. |
| S3 |
173-444 |
Sentence |
denotes |
To test the hypothesis that nitric oxide (NO) limits endothelial activation, we treated cytokine-stimulated human saphenous vein endothelial cells with several NO donors and assessed their effects on the inducible expression of vascular cell adhesion molecule-1 (VCAM-1). |
| S4 |
445-628 |
Sentence |
denotes |
In a concentration-dependent manner, NO inhibited interleukin (IL)-1 alpha-stimulated VCAM-1 expression by 35-55% as determined by cell surface enzyme immunoassays and flow cytometry. |
| S5 |
629-925 |
Sentence |
denotes |
This inhibition was paralleled by reduced monocyte adhesion to endothelial monolayers in nonstatic assays, was unaffected by cGMP analogues, and was quantitatively similar after stimulation by either IL-1 alpha, IL-1 beta, IL-4, tumor necrosis factor (TNF alpha), or bacterial lipopolysaccharide. |
| S6 |
926-1121 |
Sentence |
denotes |
NO also decreased the endothelial expression of other leukocyte adhesion molecules (E-selectin and to a lesser extent, intercellular adhesion molecule-1) and secretable cytokines (IL-6 and IL-8). |
| S7 |
1122-1282 |
Sentence |
denotes |
Inhibition of endogenous NO production by L-N-monomethyl-arginine also induced the expression of VCAM-1, but did not augment cytokine-induced VCAM-1 expression. |
| S8 |
1283-1514 |
Sentence |
denotes |
Nuclear run-on assays, transfection studies using various VCAM-1 promoter reporter gene constructs, and electrophoretic mobility shift assays indicated that NO represses VCAM-1 gene transcription, in part, by inhibiting NF-kappa B. |
| S9 |
1515-1707 |
Sentence |
denotes |
We propose that NO's ability to limit endothelial activation and inhibit monocyte adhesion may contribute to some of its antiatherogenic and antiinflammatory properties within the vessel wall. |
