pubmed-sentences-benchmark  

PubMed:1782151 JSONTXT 10 Projects

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Id Subject Object Predicate Lexical cue
S1 0-105 Sentence denotes Stimulation of interferon beta gene transcription in vitro by purified NF-kappa B and a novel TH protein.
S2 106-293 Sentence denotes The human interferon beta (IFN-beta) regulatory element consists of multiple enhanson domains which are targets for transcription factors involved in inducible expression of the promoter.
S3 294-575 Sentence denotes To further characterize the protein-DNA interactions mediating IFN-beta induction, positive regulatory domain (PRD) II binding proteins were purified from phorbol ester induced Jurkat T-cells and from IFN primed, cycloheximide/polyinosinic-polycytidylic acid treated HeLa S3 cells.
S4 576-806 Sentence denotes From HeLa cells, two major proteins of 52 and 45 kilodaltons (kD) copurified with DNA binding activity, whereas from T-cells, four proteins--a major protein of 52 kD and three minor proteins of 82, 67, and 43-47 kD--were purified.
S5 807-961 Sentence denotes Also, an induction specific DNA binding protein was purified from HeLa cells that interacted with the (AAGTGA)4 tetrahexamer sequence and the PRDI domain.
S6 962-1020 Sentence denotes This protein is immunologically distinct from IRF-1/ISGF2.
S7 1021-1159 Sentence denotes Uninduced or Sendai virus induced HeLa extracts were used to examine transcription in vitro using a series of IFN beta promoter deletions.
S8 1160-1311 Sentence denotes Deletions upstream of the PRDII element increased transcription in the uninduced extract, indicating predominantly negative regulation of the promoter.
S9 1312-1500 Sentence denotes A 2-4-fold increase in IFN-beta promoter transcription was observed in Sendai virus induced extracts, and deletion of PRDI and PRDII elements decreased this induced level of transcription.
S10 1501-1638 Sentence denotes When purified PRDII and tetrahexamer binding proteins were added to the induced extract, a 4-fold increase in transcription was observed.
S11 1639-1828 Sentence denotes These experiments demonstrate that it is possible to modulate IFN-beta transcription in vitro but indicate that additional proteins may be required to fully activate IFN-beta transcription.