| Id |
Subject |
Object |
Predicate |
Lexical cue |
| S1 |
0-105 |
Sentence |
denotes |
3-deazaadenosine, a S-adenosylhomocysteine hydrolase inhibitor, has dual effects on NF-kappaB regulation. |
| S2 |
106-197 |
Sentence |
denotes |
Inhibition of NF-kappaB transcriptional activity and promotion of IkappaBalpha degradation. |
| S3 |
198-469 |
Sentence |
denotes |
Previously we reported that 3-deazaadenosine (DZA), a potent inhibitor and substrate for S-adenosylhomocysteine hydrolase inhibits bacterial lipopolysaccharide-induced transcription of tumor necrosis factor-alpha and interleukin-1beta in mouse macrophage RAW 264.7 cells. |
| S4 |
470-567 |
Sentence |
denotes |
In this study, we demonstrate the effects of DZA on nuclear factor-kappaB (NF-kappaB) regulation. |
| S5 |
568-748 |
Sentence |
denotes |
DZA inhibits the transcriptional activity of NF-kappaB through the hindrance of p65 (Rel-A) phosphorylation without reduction of its nuclear translocation and DNA binding activity. |
| S6 |
749-863 |
Sentence |
denotes |
The inhibitory effect of DZA on NF-kappaB transcriptional activity is potentiated by the addition of homocysteine. |
| S7 |
864-1102 |
Sentence |
denotes |
Taken together, DZA promotes the proteolytic degradation of IkappaBalpha, but not IkappaBbeta, resulting in an increase of DNA binding activity of NF-kappaB in the nucleus in the absence of its transcriptional activity in RAW 264.7 cells. |
| S8 |
1103-1243 |
Sentence |
denotes |
The reduction of IkappaBalpha by DZA is neither involved in IkappaB kinase complex activation nor modulated by the addition of homocysteine. |
| S9 |
1244-1479 |
Sentence |
denotes |
This study strongly suggests that DZA may be a potent drug for the treatment of diseases in which NF-kappaB plays a central pathogenic role, as well as a useful tool for studying the regulation and physiological functions of NF-kappaB. |
