| Id |
Subject |
Object |
Predicate |
Lexical cue |
| S1 |
0-89 |
Sentence |
denotes |
Human alveolar macrophages are markedly deficient in REF-1 and AP-1 DNA binding activity. |
| S2 |
90-291 |
Sentence |
denotes |
Although many functions of human alveolar macrophages are altered compared with their precursor cell, the blood monocyte (monocyte), the reason(s) for these functional changes have not been determined. |
| S3 |
292-510 |
Sentence |
denotes |
We recently reported that human alveolar macrophages do not express AP-1 DNA binding activity (Monick, M. M., Carter, A. B., Gudmundsson, G., Geist, L. J., and Hunninghake, G. W. (1998) Am. J. Physiol. 275, L389-L397). |
| S4 |
511-709 |
Sentence |
denotes |
To determine why alveolar macrophages do not express AP-1 DNA binding activity, we first showed that there was not a decrease in expression of the FOS and JUN proteins that make up the AP-1 complex. |
| S5 |
710-937 |
Sentence |
denotes |
There was, however, a significant difference in the amounts of the nuclear protein, REF-1 (which regulates AP-1 DNA binding by altering the redox status of FOS and JUN proteins), in alveolar macrophages compared with monocytes. |
| S6 |
938-1054 |
Sentence |
denotes |
In addition, in vitro differentiation of monocytes to a macrophage-like cell resulted in decreased amounts of REF-1. |
| S7 |
1055-1197 |
Sentence |
denotes |
Finally, addition of REF-1 from activated monocytes to alveolar macrophage nuclear proteins resulted in a marked increase in AP-1 DNA binding. |
| S8 |
1198-1357 |
Sentence |
denotes |
These studies strongly suggest that the process of differentiation of monocytes into alveolar macrophages is associated with a loss of REF-1 and AP-1 activity. |
| S9 |
1358-1490 |
Sentence |
denotes |
This observation may explain, in part, some of the functional differences observed for alveolar macrophages compared with monocytes. |
