| Id |
Subject |
Object |
Predicate |
Lexical cue |
| S1 |
0-97 |
Sentence |
denotes |
Effects of diesel organic extracts on chemokine production by peripheral blood mononuclear cells. |
| S2 |
98-236 |
Sentence |
denotes |
BACKGROUND: Polyaromatic hydrocarbons (PAHs) associated with diesel exhaust particles (DEPs) are found in the atmospheric urban pollution. |
| S3 |
237-348 |
Sentence |
denotes |
Such compounds have been shown to favor IgE production, bronchial hyperresponsiveness, and airway inflammation. |
| S4 |
349-447 |
Sentence |
denotes |
Chemokines are a group of chemotactic cytokines involved in the recruitment of inflammatory cells. |
| S5 |
448-600 |
Sentence |
denotes |
OBJECTIVE: We investigated the effect of DEP-PAHs on the release and mRNA expression of IL-8, MCP-1, and RANTES by PBMCs obtained from healthy subjects. |
| S6 |
601-729 |
Sentence |
denotes |
METHODS: Protein production in supernatants was assessed by ELISA, and mRNA expression was evaluated by semiquantitative RT-PCR. |
| S7 |
730-876 |
Sentence |
denotes |
RESULTS: Secretion of IL-8 and RANTES increased in a dose-dependent manner with increasing concentrations of DEP-PAHs (range, 0.5 ng to 50 ng/mL). |
| S8 |
877-976 |
Sentence |
denotes |
On the contrary, the release of MCP-1 was significantly inhibited, also in a dose-dependent manner. |
| S9 |
977-1112 |
Sentence |
denotes |
Messenger RNA production coding for IL-8, RANTES, and MCP-1 showed parallel variations to the production of the correspondent proteins. |
| S10 |
1113-1258 |
Sentence |
denotes |
Effects of DEP-PAHs became significant at 7 hours and up to 48 hours time culture for MCP-1, and up to 24 hours time culture for IL-8 and RANTES. |
| S11 |
1259-1553 |
Sentence |
denotes |
Moreover, supernatants from DEP-PAH-activated cells, compared with those of controls, exhibited a significantly enhanced chemotactic activity for neutrophils and eosinophils, which was significantly inhibited by pretreatment with anti-IL-8 and anti-RANTES neutralizing antibodies, respectively. |
| S12 |
1554-1786 |
Sentence |
denotes |
CONCLUSION: These findings suggest that the chemokine pathways are modulated by DEP-PAHs at the transcriptional level, reinforcing the idea that the development of inflammatory reactions might be affected by diesel exhaust emission. |
