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PubMed:10328107 JSONTXT 9 Projects

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Id Subject Object Predicate Lexical cue
S1 0-135 Sentence denotes Apoptosis and growth inhibition in malignant lymphocytes after treatment with arsenic trioxide at clinically achievable concentrations.
S2 136-320 Sentence denotes BACKGROUND: Arsenic trioxide (As2O3) can induce clinical remission in patients with acute promyelocytic leukemia via induction of differentiation and programmed cell death (apoptosis).
S3 321-535 Sentence denotes We investigated the effects of As2O3 on a panel of malignant lymphocytes to determine whether growth-inhibitory and apoptotic effects of As2O3 can be observed in these cells at clinically achievable concentrations.
S4 536-782 Sentence denotes METHODS: Eight malignant lymphocytic cell lines and primary cultures of lymphocytic leukemia and lymphoma cells were treated with As2O3, with or without dithiothreitol (DTT) or buthionine sulfoximine (BSO) (an inhibitor of glutathione synthesis).
S5 783-935 Sentence denotes Apoptosis was assessed by cell morphology, flow cytometry, annexin V protein level, and terminal deoxynucleotidyl transferase labeling of DNA fragments.
S6 936-1080 Sentence denotes Cellular proliferation was determined by 5-bromo-2'-deoxyuridine incorporation into DNA and flow cytometry and by use of a mitotic arrest assay.
S7 1081-1201 Sentence denotes Mitochondrial transmembrane potential (delta psi(m)) was measured by means of rhodamine 123 staining and flow cytometry.
S8 1202-1281 Sentence denotes Protein expression was assessed by western blot analysis or immunofluorescence.
S9 1282-1520 Sentence denotes RESULTS: Therapeutic concentrations of As2O3 (1-2 microM) had dual effects on malignant lymphocytes: 1) inhibition of growth through adenosine triphosphate (ATP) depletion and prolongation of cell cycle time and 2) induction of apoptosis.
S10 1521-1583 Sentence denotes As2O3-induced apoptosis was preceded by delta psi(m) collapse.
S11 1584-1683 Sentence denotes DTT antagonized and BSO enhanced As2O3-induced ATP depletion, delta psi(m) collapse, and apoptosis.
S12 1684-1807 Sentence denotes Caspase-3 activation, usually resulting from delta psi(m) collapse, was not always associated with As2O3-induced apoptosis.
S13 1808-2073 Sentence denotes As2O3 induced PML (promyelocytic leukemia) protein degradation but did not modulate expression of cell cycle-related proteins, including c-myc, retinoblastoma protein, cyclin-dependent kinase 4, cyclin D1, and p53, or expression of differentiation-related antigens.
S14 2074-2246 Sentence denotes CONCLUSIONS: Substantial growth inhibition and apoptosis without evidence of differentiation were induced in most malignant lymphocytic cells treated with 1-2 microM As2O3.
S15 2247-2330 Sentence denotes As2O3 may prove useful in the treatment of malignant lymphoproliferative disorders.