PMC:3359311 / 25931-28194 JSONTXT 16 Projects

Annnotations TAB TSV DIC JSON TextAE

Id Subject Object Predicate Lexical cue
T9469 0-4 NNP denotes Cell
T9470 5-12 NNP denotes Culture
T9471 13-15 NN denotes CF
T9472 16-17 -LRB- denotes (
T9473 17-22 NN denotes IB3-1
T9474 23-26 CC denotes and
T9475 27-33 NN denotes CuFi-1
T9476 33-34 -RRB- denotes )
T9477 35-38 CC denotes and
T9478 39-46 NN denotes control
T9479 47-48 -LRB- denotes (
T9480 48-50 NN denotes S9
T9481 51-54 CC denotes and
T9482 55-61 NN denotes NuLi-1
T9483 61-62 -RRB- denotes )
T9484 63-68 NN denotes cells
T9485 69-73 VB denotes were
T9486 74-82 VB denotes obtained
T9487 83-87 IN denotes from
T9488 88-91 DT denotes the
T9489 92-100 NNP denotes American
T9490 101-105 NNP denotes Type
T9491 106-113 NNP denotes Culture
T9492 114-125 NNP denotes Collection.
T9493 126-131 NN denotes IB3-1
T9494 132-137 NN denotes cells
T9495 138-142 VB denotes were
T9496 143-150 VB denotes derived
T9497 151-155 IN denotes from
T9498 156-157 DT denotes a
T9499 158-165 NN denotes patient
T9500 166-176 VB denotes expressing
T9501 177-180 DT denotes the
T9502 181-186 NN denotes ΔF508
T9503 187-190 CC denotes and
T9504 191-197 NN denotes W1282X
T9505 198-207 NN denotes mutations
T9506 208-211 CC denotes and
T9507 212-218 NN denotes CuFi-1
T9508 219-223 VB denotes were
T9509 224-231 VB denotes derived
T9510 232-236 IN denotes from
T9511 237-238 DT denotes a
T9512 239-244 NN denotes ΔF508
T9513 245-255 JJ denotes homozygous
T9514 256-263 NN denotes patient
T9515 265-267 NN denotes S9
T9516 268-273 NN denotes cells
T9517 274-277 VB denotes are
T9518 278-283 NN denotes IB3-1
T9519 284-289 NN denotes cells
T9520 290-294 WDT denotes that
T9521 295-299 VB denotes have
T9522 300-304 VB denotes been
T9523 305-316 VB denotes transfected
T9524 317-321 IN denotes with
T9525 322-326 NN denotes CFTR
T9526 327-332 VB denotes using
T9527 333-335 DT denotes an
T9528 336-352 JJ denotes adeno-associated
T9529 353-358 JJ denotes viral
T9530 359-365 NN denotes vector
T9531 366-369 CC denotes and
T9532 370-376 NN denotes NuLi-1
T9533 377-382 NN denotes cells
T9534 383-387 VB denotes were
T9535 388-395 VB denotes derived
T9536 396-400 IN denotes from
T9537 401-402 DT denotes a
T9538 403-410 NN denotes patient
T9539 411-421 VB denotes possessing
T9540 422-423 DT denotes a
T9541 424-433 JJ denotes wild-type
T9542 434-438 NN denotes CFTR
T9543 439-447 NN denotes genotype
T9544 449-459 JJ denotes THP1-XBlue
T9545 460-465 NN denotes cells
T9546 466-472 RB denotes stably
T9547 473-480 VB denotes express
T9548 481-482 DT denotes a
T9549 483-491 VB denotes secreted
T9550 492-501 JJ denotes embryonic
T9551 502-510 NN denotes alkaline
T9552 511-522 NN denotes phosphatase
T9553 523-524 -LRB- denotes (
T9554 524-528 NN denotes SEAP
T9555 528-529 -RRB- denotes )
T9556 530-538 NN denotes reporter
T9557 539-548 JJ denotes inducible
T9558 549-551 IN denotes by
T9559 552-557 NN denotes NF-κB
T9560 558-561 CC denotes and
T9561 562-566 NN denotes AP-1
T9562 567-568 -LRB- denotes (
T9563 568-577 NN denotes Invivogen
T9564 577-578 -RRB- denotes )
T9565 580-585 NN denotes Cells
T9566 586-590 VB denotes were
T9567 591-599 VB denotes cultured
T9568 600-602 IN denotes as
T9569 603-614 VB denotes recommended
T9570 615-617 IN denotes by
T9571 618-623 PRP-DOLLAR- denotes their
T9572 624-634 JJ denotes respective
T9573 635-644 NN denotes suppliers
T9574 645-650 VB denotes using
T9575 651-659 JJ denotes standard
T9576 660-669 NN denotes protocols
T9577 671-673 NN denotes S9
T9578 674-677 CC denotes and
T9579 678-683 NN denotes IB3-1
T9580 684-689 NN denotes cells
T9581 690-694 VB denotes were
T9582 695-703 VB denotes cultured
T9583 704-706 IN denotes in
T9584 707-712 JJ denotes basal
T9585 713-718 NN denotes LHC-8
T9586 719-720 -LRB- denotes (
T9587 720-730 NN denotes Invitrogen
T9588 730-731 -RRB- denotes )
T9589 732-744 VB denotes supplemented
T9590 745-749 IN denotes with
T9591 750-752 CD denotes 10
T9592 752-753 NN denotes %
T9593 754-755 -LRB- denotes (
T9594 755-758 NN denotes v/v
T9595 758-759 -RRB- denotes )
T9596 760-765 JJ denotes fetal
T9597 766-772 JJ denotes bovine
T9598 773-778 NN denotes serum
T9599 779-780 -LRB- denotes (
T9600 780-783 NN denotes FBS
T9601 783-784 -RRB- denotes )
T9602 784-785 -COMMA- denotes ,
T9603 786-787 CD denotes 2
T9604 788-790 NN denotes mM
T9605 791-802 NN denotes L-glutamine
T9606 802-803 -COMMA- denotes ,
T9607 804-807 CC denotes and
T9608 808-809 CD denotes 1
T9609 810-812 NN denotes mM
T9610 813-819 NN denotes sodium
T9611 820-828 NN denotes pyruvate
T9612 830-843 NN denotes NuLi-1/CuFi-1
T9613 844-849 NN denotes cells
T9614 850-854 VB denotes were
T9615 855-863 VB denotes cultured
T9616 864-866 IN denotes in
T9617 867-871 NN denotes BEBM
T9618 872-882 JJ denotes serum-free
T9619 883-889 NN denotes medium
T9620 890-891 -LRB- denotes (
T9621 891-896 NN denotes Lonza
T9622 896-897 -RRB- denotes )
T9623 898-902 IN denotes with
T9624 903-913 NN denotes supplement
T9625 914-920 NN denotes bullet
T9626 921-924 NN denotes kit
T9627 925-926 -LRB- denotes (
T9628 926-929 NN denotes EGF
T9629 929-930 -COMMA- denotes ,
T9630 931-945 NN denotes hydrocortisone
T9631 945-946 -COMMA- denotes ,
T9632 947-953 JJ denotes bovine
T9633 954-963 JJ denotes pituitary
T9634 964-971 NN denotes extract
T9635 971-972 -COMMA- denotes ,
T9636 973-984 NN denotes transferrin
T9637 984-985 -COMMA- denotes ,
T9638 986-992 JJ denotes bovine
T9639 993-1000 NN denotes insulin
T9640 1000-1001 -COMMA- denotes ,
T9641 1002-1018 NN denotes triiodothyronine
T9642 1018-1019 -COMMA- denotes ,
T9643 1020-1031 NN denotes epinephrine
T9644 1031-1032 -COMMA- denotes ,
T9645 1033-1041 JJ denotes retinoic
T9646 1042-1046 NN denotes acid
T9647 1046-1047 -RRB- denotes )
T9648 1047-1048 -COMMA- denotes ,
T9649 1049-1050 CD denotes 2
T9650 1051-1053 NN denotes mM
T9651 1054-1065 NN denotes L-glutamine
T9652 1065-1066 -COMMA- denotes ,
T9653 1067-1070 CC denotes and
T9654 1071-1072 CD denotes 1
T9655 1073-1075 NN denotes mM
T9656 1076-1082 NN denotes sodium
T9657 1083-1091 NN denotes pyruvate
T9658 1093-1098 NN denotes PBMCs
T9659 1099-1103 IN denotes from
T9660 1104-1106 NN denotes CF
T9661 1107-1115 NN denotes patients
T9662 1116-1119 CC denotes and
T9663 1120-1128 NN denotes controls
T9664 1129-1133 VB denotes were
T9665 1134-1142 VB denotes cultured
T9666 1143-1145 IN denotes in
T9667 1146-1155 NN denotes RPMI-1640
T9668 1156-1157 -LRB- denotes (
T9669 1157-1164 NN denotes Hyclone
T9670 1164-1165 -RRB- denotes )
T9671 1166-1178 VB denotes supplemented
T9672 1179-1183 IN denotes with
T9673 1184-1186 CD denotes 10
T9674 1186-1187 NN denotes %
T9675 1188-1191 NNP denotes FBS
T9676 1191-1192 -COMMA- denotes ,
T9677 1193-1194 CD denotes 2
T9678 1195-1197 NN denotes mM
T9679 1198-1209 NN denotes L-glutamine
T9680 1209-1210 -COMMA- denotes ,
T9681 1211-1214 CC denotes and
T9682 1215-1216 CD denotes 1
T9683 1217-1219 NN denotes mM
T9684 1220-1226 NN denotes sodium
T9685 1227-1235 NN denotes pyruvate
T9686 1236-1237 -LRB- denotes (
T9687 1237-1245 JJ denotes complete
T9688 1246-1250 NN denotes RPMI
T9689 1250-1251 -RRB- denotes )
T9690 1253-1263 JJ denotes THP1-XBlue
T9691 1264-1269 NN denotes cells
T9692 1270-1274 VB denotes were
T9693 1275-1283 VB denotes cultured
T9694 1284-1286 IN denotes in
T9695 1287-1295 JJ denotes complete
T9696 1296-1300 NN denotes RPMI
T9697 1301-1305 IN denotes with
T9698 1306-1309 DT denotes the
T9699 1310-1318 NN denotes addition
T9700 1319-1321 IN denotes of
T9701 1322-1328 NN denotes zeocin
T9702 1329-1330 -LRB- denotes (
T9703 1330-1333 CD denotes 100
T9704 1334-1339 NN denotes µg/ml
T9705 1339-1340 -RRB- denotes )
T9706 1341-1343 TO denotes to
T9707 1344-1350 VB denotes select
T9708 1351-1354 IN denotes for
T9709 1355-1360 NN denotes cells
T9710 1361-1371 VB denotes expressing
T9711 1372-1375 DT denotes the
T9712 1376-1380 NN denotes SEAP
T9713 1381-1391 NN denotes NF-κB/AP-1
T9714 1392-1400 NN denotes reporter
T9715 1402-1407 RB denotes Prior
T9716 1408-1410 TO denotes to
T9717 1411-1422 NN denotes stimulation
T9718 1422-1423 -COMMA- denotes ,
T9719 1424-1433 JJ denotes bronchial
T9720 1434-1444 JJ denotes epithelial
T9721 1445-1449 NN denotes cell
T9722 1450-1455 NN denotes lines
T9723 1456-1460 VB denotes were
T9724 1461-1467 VB denotes plated
T9725 1468-1470 IN denotes in
T9726 1471-1477 VB denotes coated
T9727 1478-1479 -LRB- denotes [
T9728 1479-1481 CD denotes 62
T9729 1481-1482 -RRB- denotes ]
T9730 1483-1490 JJ denotes 96-well
T9731 1491-1497 NN denotes plates
T9732 1498-1499 -LRB- denotes (
T9733 1499-1501 NN denotes BD
T9734 1502-1513 NNP denotes Biosciences
T9735 1513-1514 -RRB- denotes )
T9736 1515-1517 IN denotes at
T9737 1518-1523 CD denotes 3×104
T9738 1524-1534 NN denotes cells/well
T9739 1535-1541 IN denotes unless
T9740 1542-1551 VB denotes indicated
T9741 1551-1552 -COMMA- denotes ,
T9742 1553-1556 CC denotes and
T9743 1557-1564 VB denotes allowed
T9744 1565-1567 TO denotes to
T9745 1568-1574 VB denotes adhere
T9746 1575-1584 RB denotes overnight
T9747 1586-1592 NN denotes Plates
T9748 1593-1596 IN denotes for
T9749 1597-1599 NN denotes S9
T9750 1600-1603 CC denotes and
T9751 1604-1609 NN denotes IB3-1
T9752 1610-1622 NN denotes stimulations
T9753 1623-1627 VB denotes were
T9754 1628-1634 VB denotes coated
T9755 1635-1637 IN denotes in
T9756 1638-1639 DT denotes a
T9757 1640-1647 NN denotes mixture
T9758 1648-1650 IN denotes of
T9759 1651-1657 JJ denotes bovine
T9760 1658-1663 NN denotes serum
T9761 1664-1671 NN denotes albumin
T9762 1672-1673 -LRB- denotes (
T9763 1673-1676 CD denotes 100
T9764 1677-1682 NN denotes µg/ml
T9765 1682-1683 -RRB- denotes )
T9766 1683-1684 -COMMA- denotes ,
T9767 1685-1696 NN denotes fibronectin
T9768 1697-1698 -LRB- denotes (
T9769 1698-1700 CD denotes 10
T9770 1701-1706 NN denotes µg/ml
T9771 1706-1707 -RRB- denotes )
T9772 1707-1708 -COMMA- denotes ,
T9773 1709-1712 CC denotes and
T9774 1713-1719 JJ denotes bovine
T9775 1720-1727 NN denotes collage
T9776 1728-1732 NN denotes type
T9777 1733-1734 CD denotes I
T9778 1735-1736 -LRB- denotes (
T9779 1736-1738 CD denotes 30
T9780 1739-1744 NN denotes µg/ml
T9781 1744-1745 -RRB- denotes )
T9782 1746-1747 -LRB- denotes (
T9783 1747-1749 NN denotes BD
T9784 1750-1761 NNP denotes Biosciences
T9785 1761-1762 -RRB- denotes )
T9786 1764-1770 NN denotes Plates
T9787 1771-1774 IN denotes for
T9788 1775-1781 NN denotes NuLi-1
T9789 1782-1785 CC denotes and
T9790 1786-1792 NN denotes CuFi-1
T9791 1793-1797 VB denotes were
T9792 1798-1804 VB denotes coated
T9793 1805-1809 IN denotes with
T9794 1810-1818 NN denotes collagen
T9795 1819-1823 NN denotes type
T9796 1824-1826 CD denotes IV
T9797 1827-1828 -LRB- denotes (
T9798 1828-1830 CD denotes 60
T9799 1831-1836 NN denotes µg/ml
T9800 1836-1837 -RRB- denotes )
T9801 1838-1839 -LRB- denotes (
T9802 1839-1844 NNP denotes Sigma
T9803 1845-1852 NNP denotes Aldrich
T9804 1852-1853 -RRB- denotes )
T9805 1855-1860 NN denotes PBMCs
T9806 1861-1865 VB denotes were
T9807 1866-1872 VB denotes plated
T9808 1873-1875 IN denotes in
T9809 1876-1883 JJ denotes 96-well
T9810 1884-1890 NN denotes plates
T9811 1891-1893 IN denotes at
T9812 1894-1895 DT denotes a
T9813 1896-1903 NN denotes density
T9814 1904-1906 IN denotes of
T9815 1907-1914 CD denotes 1.5×105
T9816 1915-1925 NN denotes cells/well
T9817 1926-1928 IN denotes in
T9818 1929-1932 CD denotes 200
T9819 1933-1935 NN denotes µl
T9820 1936-1937 -LRB- denotes (
T9821 1937-1944 CD denotes 7.5×105
T9822 1945-1953 NN denotes cells/mL
T9823 1953-1954 -RRB- denotes )
T9824 1956-1961 NN denotes THP-1
T9825 1962-1970 NN denotes reporter
T9826 1971-1976 NN denotes cells
T9827 1977-1981 VB denotes were
T9828 1982-1996 VB denotes differentiated
T9829 1997-2001 IN denotes into
T9830 2002-2003 DT denotes a
T9831 2004-2019 JJ denotes macrophage-like
T9832 2020-2029 NN denotes phenotype
T9833 2030-2035 VB denotes using
T9834 2036-2038 CD denotes 50
T9835 2039-2044 NN denotes ng/ml
T9836 2045-2047 IN denotes of
T9837 2048-2055 NN denotes phorbol
T9838 2056-2068 NN denotes 12-myristate
T9839 2069-2079 NN denotes 13-acetate
T9840 2080-2081 -LRB- denotes (
T9841 2081-2084 NN denotes PMA
T9842 2084-2085 -RRB- denotes )
T9843 2086-2087 -LRB- denotes (
T9844 2087-2092 NNP denotes Sigma
T9845 2093-2100 NNP denotes Aldrich
T9846 2100-2101 -RRB- denotes )
T9847 2102-2105 IN denotes for
T9848 2106-2108 CD denotes 24
T9849 2109-2114 NN denotes hours
T9850 2115-2117 IN denotes at
T9851 2118-2119 DT denotes a
T9852 2120-2127 NN denotes density
T9853 2128-2130 IN denotes of
T9854 2131-2136 CD denotes 1×105
T9855 2137-2147 NN denotes cells/well
T9856 2148-2150 IN denotes in
T9857 2151-2154 CD denotes 200
T9858 2155-2157 NN denotes µl
T9859 2158-2159 -LRB- denotes (
T9860 2159-2164 CD denotes 5×105
T9861 2165-2173 NN denotes cells/ml
T9862 2173-2174 -RRB- denotes )
T9863 2176-2181 NN denotes Cells
T9864 2182-2186 VB denotes were
T9865 2187-2193 VB denotes washed
T9866 2194-2198 IN denotes with
T9867 2199-2202 NNP denotes PBS
T9868 2203-2206 CC denotes and
T9869 2207-2214 VB denotes allowed
T9870 2215-2217 TO denotes to
T9871 2218-2222 VB denotes rest
T9872 2223-2224 DT denotes a
T9873 2225-2232 JJ denotes further
T9874 2233-2235 CD denotes 42
T9875 2236-2241 NN denotes hours
T9876 2242-2247 RB denotes prior
T9877 2248-2250 TO denotes to
T9878 2251-2262 NN denotes stimulation
R8253 T9470 T9469 arg1Of Culture,Cell
R8254 T9471 T9472 arg1Of CF,(
R8255 T9471 T9477 arg1Of CF,and
R8256 T9473 T9474 arg1Of IB3-1,and
R8257 T9474 T9472 arg2Of and,(
R8258 T9475 T9474 arg2Of CuFi-1,and
R8259 T9476 T9472 arg3Of ),(
R8260 T9477 T9495 arg1Of and,were
R8261 T9477 T9496 arg2Of and,derived
R8262 T9478 T9477 arg2Of control,and
R8263 T9478 T9479 arg1Of control,(
R8264 T9480 T9481 arg1Of S9,and
R8265 T9481 T9479 arg2Of and,(
R8266 T9482 T9481 arg2Of NuLi-1,and
R8267 T9483 T9479 arg3Of ),(
R8268 T9484 T9485 arg1Of cells,were
R8269 T9484 T9486 arg2Of cells,obtained
R8270 T9486 T9485 arg2Of obtained,were
R8271 T9486 T9487 arg1Of obtained,from
R8272 T9492 T9489 arg1Of Collection.,American
R8273 T9492 T9490 arg1Of Collection.,Type
R8274 T9492 T9491 arg1Of Collection.,Culture
R8275 T9494 T9487 arg2Of cells,from
R8276 T9494 T9488 arg1Of cells,the
R8277 T9494 T9492 arg1Of cells,Collection.
R8278 T9494 T9493 arg1Of cells,IB3-1
R8279 T9496 T9485 modOf derived,were
R8280 T9496 T9495 arg2Of derived,were
R8281 T9496 T9497 arg1Of derived,from
R8282 T9496 T9506 arg1Of derived,and
R8283 T9499 T9497 arg2Of patient,from
R8284 T9499 T9498 arg1Of patient,a
R8285 T9499 T9500 arg1Of patient,expressing
R8286 T9502 T9503 arg1Of ΔF508,and
R8287 T9504 T9503 arg2Of W1282X,and
R8288 T9505 T9500 arg2Of mutations,expressing
R8289 T9505 T9501 arg1Of mutations,the
R8290 T9505 T9502 arg1Of mutations,ΔF508
R8291 T9505 T9504 arg1Of mutations,W1282X
R8292 T9507 T9508 arg1Of CuFi-1,were
R8293 T9507 T9509 arg2Of CuFi-1,derived
R8294 T9509 T9506 arg2Of derived,and
R8295 T9509 T9508 arg2Of derived,were
R8296 T9509 T9510 arg1Of derived,from
R8297 T9514 T9510 arg2Of patient,from
R8298 T9514 T9511 arg1Of patient,a
R8299 T9514 T9512 arg1Of patient,ΔF508
R8300 T9514 T9513 arg1Of patient,homozygous
R8301 T9516 T9515 arg1Of cells,S9
R8302 T9516 T9517 arg1Of cells,are
R8303 T9517 T9531 arg1Of are,and
R8304 T9519 T9517 arg2Of cells,are
R8305 T9519 T9518 arg1Of cells,IB3-1
R8306 T9519 T9520 arg1Of cells,that
R8307 T9519 T9521 arg1Of cells,have
R8308 T9519 T9522 arg1Of cells,been
R8309 T9519 T9523 arg2Of cells,transfected
R8310 T9523 T9521 arg2Of transfected,have
R8311 T9523 T9522 arg2Of transfected,been
R8312 T9523 T9524 arg1Of transfected,with
R8313 T9525 T9524 arg2Of CFTR,with
R8314 T9525 T9526 arg1Of CFTR,using
R8315 T9530 T9526 arg2Of vector,using
R8316 T9530 T9527 arg1Of vector,an
R8317 T9530 T9528 arg1Of vector,adeno-associated
R8318 T9530 T9529 arg1Of vector,viral
R8319 T9533 T9532 arg1Of cells,NuLi-1
R8320 T9533 T9534 arg1Of cells,were
R8321 T9533 T9535 arg2Of cells,derived
R8322 T9535 T9531 arg2Of derived,and
R8323 T9535 T9534 arg2Of derived,were
R8324 T9535 T9536 arg1Of derived,from
R8325 T9538 T9536 arg2Of patient,from
R8326 T9538 T9537 arg1Of patient,a
R8327 T9538 T9539 arg1Of patient,possessing
R8328 T9543 T9539 arg2Of genotype,possessing
R8329 T9543 T9540 arg1Of genotype,a
R8330 T9543 T9541 arg1Of genotype,wild-type
R8331 T9543 T9542 arg1Of genotype,CFTR
R8332 T9545 T9544 arg1Of cells,THP1-XBlue
R8333 T9545 T9547 arg1Of cells,express
R8334 T9547 T9546 arg1Of express,stably
R8335 T9554 T9553 arg2Of SEAP,(
R8336 T9555 T9553 arg3Of ),(
R8337 T9556 T9547 arg2Of reporter,express
R8338 T9556 T9548 arg1Of reporter,a
R8339 T9556 T9549 arg2Of reporter,secreted
R8340 T9556 T9550 arg1Of reporter,embryonic
R8341 T9556 T9551 arg1Of reporter,alkaline
R8342 T9556 T9552 arg1Of reporter,phosphatase
R8343 T9556 T9553 arg1Of reporter,(
R8344 T9556 T9557 arg1Of reporter,inducible
R8345 T9557 T9558 arg1Of inducible,by
R8346 T9559 T9560 arg1Of NF-κB,and
R8347 T9560 T9558 arg2Of and,by
R8348 T9560 T9562 arg1Of and,(
R8349 T9561 T9560 arg2Of AP-1,and
R8350 T9563 T9562 arg2Of Invivogen,(
R8351 T9564 T9562 arg3Of ),(
R8352 T9565 T9566 arg1Of Cells,were
R8353 T9565 T9567 arg2Of Cells,cultured
R8354 T9567 T9566 arg2Of cultured,were
R8355 T9567 T9568 arg1Of cultured,as
R8356 T9567 T9574 modOf cultured,using
R8357 T9569 T9568 arg2Of recommended,as
R8358 T9573 T9569 arg1Of suppliers,recommended
R8359 T9573 T9570 arg2Of suppliers,by
R8360 T9573 T9571 arg1Of suppliers,their
R8361 T9573 T9572 arg1Of suppliers,respective
R8362 T9576 T9574 arg2Of protocols,using
R8363 T9576 T9575 arg1Of protocols,standard
R8364 T9577 T9578 arg1Of S9,and
R8365 T9579 T9578 arg2Of IB3-1,and
R8366 T9580 T9577 arg1Of cells,S9
R8367 T9580 T9579 arg1Of cells,IB3-1
R8368 T9580 T9581 arg1Of cells,were
R8369 T9580 T9582 arg2Of cells,cultured
R8370 T9582 T9581 arg2Of cultured,were
R8371 T9582 T9583 arg1Of cultured,in
R8372 T9585 T9584 arg1Of LHC-8,basal
R8373 T9585 T9586 arg1Of LHC-8,(
R8374 T9585 T9589 arg2Of LHC-8,supplemented
R8375 T9587 T9586 arg2Of Invitrogen,(
R8376 T9588 T9586 arg3Of ),(
R8377 T9589 T9590 arg1Of supplemented,with
R8378 T9592 T9590 arg2Of %,with
R8379 T9592 T9591 arg1Of %,10
R8380 T9594 T9593 arg2Of v/v,(
R8381 T9595 T9593 arg3Of ),(
R8382 T9598 T9585 arg1Of serum,LHC-8
R8383 T9598 T9593 arg1Of serum,(
R8384 T9598 T9596 arg1Of serum,fetal
R8385 T9598 T9597 arg1Of serum,bovine
R8386 T9598 T9599 arg1Of serum,(
R8387 T9598 T9602 arg1Of serum,","
R8388 T9600 T9599 arg2Of FBS,(
R8389 T9601 T9599 arg3Of ),(
R8390 T9602 T9607 arg1Of ",",and
R8391 T9603 T9604 arg1Of 2,mM
R8392 T9605 T9602 arg2Of L-glutamine,","
R8393 T9605 T9603 arg1Of L-glutamine,2
R8394 T9607 T9583 arg2Of and,in
R8395 T9607 T9606 arg1Of and,","
R8396 T9608 T9609 arg1Of 1,mM
R8397 T9611 T9607 arg2Of pyruvate,and
R8398 T9611 T9608 arg1Of pyruvate,1
R8399 T9611 T9610 arg1Of pyruvate,sodium
R8400 T9613 T9612 arg1Of cells,NuLi-1/CuFi-1
R8401 T9613 T9614 arg1Of cells,were
R8402 T9613 T9615 arg2Of cells,cultured
R8403 T9615 T9614 arg2Of cultured,were
R8404 T9615 T9616 arg1Of cultured,in
R8405 T9619 T9617 arg1Of medium,BEBM
R8406 T9619 T9618 arg1Of medium,serum-free
R8407 T9619 T9620 arg1Of medium,(
R8408 T9619 T9623 arg1Of medium,with
R8409 T9619 T9653 arg1Of medium,and
R8410 T9621 T9620 arg2Of Lonza,(
R8411 T9622 T9620 arg3Of ),(
R8412 T9626 T9624 arg1Of kit,supplement
R8413 T9626 T9625 arg1Of kit,bullet
R8414 T9626 T9627 arg1Of kit,(
R8415 T9626 T9648 arg1Of kit,","
R8416 T9628 T9629 arg1Of EGF,","
R8417 T9628 T9631 arg1Of EGF,","
R8418 T9628 T9635 arg1Of EGF,","
R8419 T9628 T9637 arg1Of EGF,","
R8420 T9630 T9629 arg2Of hydrocortisone,","
R8421 T9634 T9631 arg2Of extract,","
R8422 T9634 T9632 arg1Of extract,bovine
R8423 T9634 T9633 arg1Of extract,pituitary
R8424 T9636 T9635 arg2Of transferrin,","
R8425 T9637 T9627 arg2Of ",",(
R8426 T9637 T9640 arg1Of ",",","
R8427 T9639 T9637 arg2Of insulin,","
R8428 T9639 T9638 arg1Of insulin,bovine
R8429 T9641 T9640 arg2Of triiodothyronine,","
R8430 T9641 T9642 arg1Of triiodothyronine,","
R8431 T9641 T9644 arg1Of triiodothyronine,","
R8432 T9643 T9642 arg2Of epinephrine,","
R8433 T9646 T9644 arg2Of acid,","
R8434 T9646 T9645 arg1Of acid,retinoic
R8435 T9647 T9627 arg3Of ),(
R8436 T9648 T9623 arg2Of ",",with
R8437 T9649 T9650 arg1Of 2,mM
R8438 T9651 T9648 arg2Of L-glutamine,","
R8439 T9651 T9649 arg1Of L-glutamine,2
R8440 T9653 T9616 arg2Of and,in
R8441 T9653 T9652 arg1Of and,","
R8442 T9654 T9655 arg1Of 1,mM
R8443 T9657 T9653 arg2Of pyruvate,and
R8444 T9657 T9654 arg1Of pyruvate,1
R8445 T9657 T9656 arg1Of pyruvate,sodium
R8446 T9658 T9659 arg1Of PBMCs,from
R8447 T9658 T9664 arg1Of PBMCs,were
R8448 T9658 T9665 arg2Of PBMCs,cultured
R8449 T9661 T9660 arg1Of patients,CF
R8450 T9661 T9662 arg1Of patients,and
R8451 T9662 T9659 arg2Of and,from
R8452 T9663 T9662 arg2Of controls,and
R8453 T9665 T9664 arg2Of cultured,were
R8454 T9665 T9666 arg1Of cultured,in
R8455 T9667 T9668 arg1Of RPMI-1640,(
R8456 T9667 T9671 arg2Of RPMI-1640,supplemented
R8457 T9667 T9681 arg1Of RPMI-1640,and
R8458 T9669 T9668 arg2Of Hyclone,(
R8459 T9670 T9668 arg3Of ),(
R8460 T9671 T9672 arg1Of supplemented,with
R8461 T9674 T9673 arg1Of %,10
R8462 T9675 T9672 arg2Of FBS,with
R8463 T9675 T9674 arg1Of FBS,%
R8464 T9675 T9676 arg1Of FBS,","
R8465 T9677 T9678 arg1Of 2,mM
R8466 T9679 T9676 arg2Of L-glutamine,","
R8467 T9679 T9677 arg1Of L-glutamine,2
R8468 T9681 T9666 arg2Of and,in
R8469 T9681 T9680 arg1Of and,","
R8470 T9682 T9683 arg1Of 1,mM
R8471 T9685 T9681 arg2Of pyruvate,and
R8472 T9685 T9682 arg1Of pyruvate,1
R8473 T9685 T9684 arg1Of pyruvate,sodium
R8474 T9685 T9686 arg1Of pyruvate,(
R8475 T9688 T9686 arg2Of RPMI,(
R8476 T9688 T9687 arg1Of RPMI,complete
R8477 T9689 T9686 arg3Of ),(
R8478 T9691 T9690 arg1Of cells,THP1-XBlue
R8479 T9691 T9692 arg1Of cells,were
R8480 T9691 T9693 arg2Of cells,cultured
R8481 T9693 T9692 arg2Of cultured,were
R8482 T9693 T9694 arg1Of cultured,in
R8483 T9693 T9697 arg1Of cultured,with
R8484 T9696 T9694 arg2Of RPMI,in
R8485 T9696 T9695 arg1Of RPMI,complete
R8486 T9699 T9697 arg2Of addition,with
R8487 T9699 T9698 arg1Of addition,the
R8488 T9699 T9700 arg1Of addition,of
R8489 T9699 T9706 modOf addition,to
R8490 T9701 T9700 arg2Of zeocin,of
R8491 T9701 T9702 arg1Of zeocin,(
R8492 T9704 T9702 arg2Of µg/ml,(
R8493 T9704 T9703 arg1Of µg/ml,100
R8494 T9705 T9702 arg3Of ),(
R8495 T9707 T9706 arg1Of select,to
R8496 T9707 T9708 arg1Of select,for
R8497 T9709 T9708 arg2Of cells,for
R8498 T9709 T9710 arg1Of cells,expressing
R8499 T9714 T9710 arg2Of reporter,expressing
R8500 T9714 T9711 arg1Of reporter,the
R8501 T9714 T9712 arg1Of reporter,SEAP
R8502 T9714 T9713 arg1Of reporter,NF-κB/AP-1
R8503 T9715 T9716 arg1Of Prior,to
R8504 T9717 T9716 arg2Of stimulation,to
R8505 T9722 T9719 arg1Of lines,bronchial
R8506 T9722 T9720 arg1Of lines,epithelial
R8507 T9722 T9721 arg1Of lines,cell
R8508 T9722 T9723 arg1Of lines,were
R8509 T9722 T9724 arg2Of lines,plated
R8510 T9724 T9715 arg1Of plated,Prior
R8511 T9724 T9718 arg1Of plated,","
R8512 T9724 T9723 arg2Of plated,were
R8513 T9724 T9725 arg1Of plated,in
R8514 T9724 T9736 arg1Of plated,at
R8515 T9724 T9739 arg1Of plated,unless
R8516 T9728 T9727 arg2Of 62,[
R8517 T9729 T9727 arg3Of ],[
R8518 T9731 T9725 arg2Of plates,in
R8519 T9731 T9726 arg1Of plates,coated
R8520 T9731 T9727 arg1Of plates,[
R8521 T9731 T9730 arg1Of plates,96-well
R8522 T9731 T9732 arg1Of plates,(
R8523 T9733 T9732 arg2Of BD,(
R8524 T9733 T9734 arg1Of BD,Biosciences
R8525 T9735 T9732 arg3Of ),(
R8526 T9738 T9736 arg2Of cells/well,at
R8527 T9738 T9737 arg1Of cells/well,3×104
R8528 T9740 T9742 arg1Of indicated,and
R8529 T9742 T9739 arg2Of and,unless
R8530 T9742 T9741 arg1Of and,","
R8531 T9743 T9742 arg2Of allowed,and
R8532 T9745 T9743 arg3Of adhere,allowed
R8533 T9745 T9744 arg1Of adhere,to
R8534 T9745 T9746 arg1Of adhere,overnight
R8535 T9747 T9748 arg1Of Plates,for
R8536 T9747 T9753 arg1Of Plates,were
R8537 T9747 T9754 arg2Of Plates,coated
R8538 T9749 T9750 arg1Of S9,and
R8539 T9751 T9750 arg2Of IB3-1,and
R8540 T9752 T9748 arg2Of stimulations,for
R8541 T9752 T9749 arg1Of stimulations,S9
R8542 T9752 T9751 arg1Of stimulations,IB3-1
R8543 T9754 T9753 arg2Of coated,were
R8544 T9754 T9755 arg1Of coated,in
R8545 T9757 T9755 arg2Of mixture,in
R8546 T9757 T9756 arg1Of mixture,a
R8547 T9757 T9758 arg1Of mixture,of
R8548 T9761 T9759 arg1Of albumin,bovine
R8549 T9761 T9760 arg1Of albumin,serum
R8550 T9761 T9762 arg1Of albumin,(
R8551 T9761 T9766 arg1Of albumin,","
R8552 T9764 T9762 arg2Of µg/ml,(
R8553 T9764 T9763 arg1Of µg/ml,100
R8554 T9765 T9762 arg3Of ),(
R8555 T9766 T9773 arg1Of ",",and
R8556 T9767 T9766 arg2Of fibronectin,","
R8557 T9767 T9768 arg1Of fibronectin,(
R8558 T9770 T9768 arg2Of µg/ml,(
R8559 T9770 T9769 arg1Of µg/ml,10
R8560 T9771 T9768 arg3Of ),(
R8561 T9773 T9758 arg2Of and,of
R8562 T9773 T9772 arg1Of and,","
R8563 T9773 T9782 arg1Of and,(
R8564 T9776 T9773 arg2Of type,and
R8565 T9776 T9774 arg1Of type,bovine
R8566 T9776 T9775 arg1Of type,collage
R8567 T9776 T9777 arg1Of type,I
R8568 T9776 T9778 arg1Of type,(
R8569 T9780 T9778 arg2Of µg/ml,(
R8570 T9780 T9779 arg1Of µg/ml,30
R8571 T9781 T9778 arg3Of ),(
R8572 T9784 T9782 arg2Of Biosciences,(
R8573 T9784 T9783 arg1Of Biosciences,BD
R8574 T9785 T9782 arg3Of ),(
R8575 T9786 T9787 arg1Of Plates,for
R8576 T9786 T9791 arg1Of Plates,were
R8577 T9786 T9792 arg2Of Plates,coated
R8578 T9788 T9789 arg1Of NuLi-1,and
R8579 T9789 T9787 arg2Of and,for
R8580 T9790 T9789 arg2Of CuFi-1,and
R8581 T9792 T9791 arg2Of coated,were
R8582 T9792 T9793 arg1Of coated,with
R8583 T9795 T9793 arg2Of type,with
R8584 T9795 T9794 arg1Of type,collagen
R8585 T9795 T9796 arg1Of type,IV
R8586 T9795 T9797 arg1Of type,(
R8587 T9795 T9801 arg1Of type,(
R8588 T9799 T9797 arg2Of µg/ml,(
R8589 T9799 T9798 arg1Of µg/ml,60
R8590 T9800 T9797 arg3Of ),(
R8591 T9803 T9801 arg2Of Aldrich,(
R8592 T9803 T9802 arg1Of Aldrich,Sigma
R8593 T9804 T9801 arg3Of ),(
R8594 T9805 T9806 arg1Of PBMCs,were
R8595 T9805 T9807 arg2Of PBMCs,plated
R8596 T9807 T9806 arg2Of plated,were
R8597 T9807 T9808 arg1Of plated,in
R8598 T9807 T9811 arg1Of plated,at
R8599 T9810 T9808 arg2Of plates,in
R8600 T9810 T9809 arg1Of plates,96-well
R8601 T9813 T9811 arg2Of density,at
R8602 T9813 T9812 arg1Of density,a
R8603 T9813 T9814 arg1Of density,of
R8604 T9813 T9817 arg1Of density,in
R8605 T9816 T9814 arg2Of cells/well,of
R8606 T9816 T9815 arg1Of cells/well,1.5×105
R8607 T9819 T9817 arg2Of µl,in
R8608 T9819 T9818 arg1Of µl,200
R8609 T9819 T9820 arg1Of µl,(
R8610 T9822 T9820 arg2Of cells/mL,(
R8611 T9822 T9821 arg1Of cells/mL,7.5×105
R8612 T9823 T9820 arg3Of ),(
R8613 T9826 T9824 arg1Of cells,THP-1
R8614 T9826 T9825 arg1Of cells,reporter
R8615 T9826 T9827 arg1Of cells,were
R8616 T9826 T9828 arg2Of cells,differentiated
R8617 T9828 T9827 arg2Of differentiated,were
R8618 T9828 T9829 arg1Of differentiated,into
R8619 T9832 T9829 arg2Of phenotype,into
R8620 T9832 T9830 arg1Of phenotype,a
R8621 T9832 T9831 arg1Of phenotype,macrophage-like
R8622 T9832 T9833 arg1Of phenotype,using
R8623 T9833 T9847 arg1Of using,for
R8624 T9835 T9833 arg2Of ng/ml,using
R8625 T9835 T9834 arg1Of ng/ml,50
R8626 T9835 T9836 arg1Of ng/ml,of
R8627 T9839 T9836 arg2Of 13-acetate,of
R8628 T9839 T9837 arg1Of 13-acetate,phorbol
R8629 T9839 T9838 arg1Of 13-acetate,12-myristate
R8630 T9839 T9840 arg1Of 13-acetate,(
R8631 T9839 T9843 arg1Of 13-acetate,(
R8632 T9841 T9840 arg2Of PMA,(
R8633 T9842 T9840 arg3Of ),(
R8634 T9845 T9843 arg2Of Aldrich,(
R8635 T9845 T9844 arg1Of Aldrich,Sigma
R8636 T9846 T9843 arg3Of ),(
R8637 T9849 T9847 arg2Of hours,for
R8638 T9849 T9848 arg1Of hours,24
R8639 T9849 T9850 arg1Of hours,at
R8640 T9852 T9850 arg2Of density,at
R8641 T9852 T9851 arg1Of density,a
R8642 T9852 T9853 arg1Of density,of
R8643 T9852 T9856 arg1Of density,in
R8644 T9855 T9853 arg2Of cells/well,of
R8645 T9855 T9854 arg1Of cells/well,1×105
R8646 T9858 T9856 arg2Of µl,in
R8647 T9858 T9857 arg1Of µl,200
R8648 T9858 T9859 arg1Of µl,(
R8649 T9861 T9859 arg2Of cells/ml,(
R8650 T9861 T9860 arg1Of cells/ml,5×105
R8651 T9862 T9859 arg3Of ),(
R8652 T9863 T9864 arg1Of Cells,were
R8653 T9863 T9865 arg2Of Cells,washed
R8654 T9863 T9869 arg2Of Cells,allowed
R8655 T9863 T9871 arg1Of Cells,rest
R8656 T9865 T9866 arg1Of washed,with
R8657 T9865 T9868 arg1Of washed,and
R8658 T9867 T9866 arg2Of PBS,with
R8659 T9868 T9864 arg2Of and,were
R8660 T9869 T9868 arg2Of allowed,and
R8661 T9871 T9869 arg3Of rest,allowed
R8662 T9871 T9870 arg1Of rest,to
R8663 T9871 T9876 arg1Of rest,prior
R8664 T9875 T9871 arg2Of hours,rest
R8665 T9875 T9872 arg1Of hours,a
R8666 T9875 T9873 arg1Of hours,further
R8667 T9875 T9874 arg1Of hours,42
R8668 T9876 T9877 arg1Of prior,to
R8669 T9878 T9877 arg2Of stimulation,to