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PMC:3342329 / 33686-34891 JSONTXT 23 Projects

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Id Subject Object Predicate Lexical cue
T16223 0-4 NN denotes Flow
T16224 5-15 JJ denotes Cytometric
T16225 16-24 NN denotes Analysis
T16226 25-36 NN denotes Lymphocytes
T16227 37-41 VB denotes were
T16228 42-50 VB denotes obtained
T16229 51-55 IN denotes from
T16230 56-61 NN denotes tumor
T16231 62-65 CC denotes and
T16232 66-72 NN denotes spleen
T16233 73-80 NN denotes tissues
T16234 81-84 IN denotes for
T16235 85-94 NN denotes phenotype
T16236 95-103 NN denotes analysis
T16237 105-111 NN denotes Spleen
T16238 112-115 CC denotes and
T16239 116-121 NN denotes tumor
T16240 122-133 NN denotes lymphocytes
T16241 134-138 VB denotes were
T16242 139-147 VB denotes isolated
T16243 148-152 IN denotes from
T16244 153-158 JJ denotes fresh
T16245 159-165 NN denotes spleen
T16246 166-169 CC denotes and
T16247 170-175 NN denotes tumor
T16248 176-184 NN denotes biopsies
T16249 185-193 VB denotes obtained
T16250 194-198 IN denotes from
T16251 199-204 CD denotes three
T16252 205-209 NN denotes mice
T16253 210-213 IN denotes per
T16254 214-219 NN denotes group
T16255 221-227 NN denotes Spleen
T16256 228-231 CC denotes and
T16257 232-237 NN denotes tumor
T16258 238-246 NN denotes biopsies
T16259 247-251 VB denotes were
T16260 252-259 RB denotes briefly
T16261 260-271 VB denotes homogenized
T16262 272-284 RB denotes mechanically
T16263 285-287 IN denotes in
T16264 288-291 NNP denotes PBS
T16265 291-292 -COMMA- denotes ,
T16266 293-301 VB denotes filtered
T16267 302-303 -LRB- denotes (
T16268 303-306 CD denotes 100
T16269 307-309 NN denotes µm
T16270 310-314 NN denotes cell
T16271 315-323 NN denotes strainer
T16272 323-324 -COLON- denotes ;
T16273 325-327 NN denotes BD
T16274 328-338 NN denotes PharMingen
T16275 338-339 -RRB- denotes )
T16276 340-343 CC denotes and
T16277 344-348 RB denotes then
T16278 349-355 VB denotes placed
T16279 356-358 IN denotes in
T16280 359-364 NN denotes lysis
T16281 365-371 NN denotes buffer
T16282 372-373 -LRB- denotes (
T16283 373-376 NN denotes ACK
T16284 377-383 NN denotes lysing
T16285 384-390 NN denotes buffer
T16286 390-391 -COMMA- denotes ,
T16287 392-397 NN denotes LONZA
T16288 397-398 -RRB- denotes )
T16289 399-401 TO denotes to
T16290 402-408 VB denotes remove
T16291 409-412 JJ denotes red
T16292 413-418 NN denotes blood
T16293 419-424 NN denotes cells
T16294 426-429 CD denotes One
T16295 430-437 CD denotes million
T16296 438-449 NN denotes splenocytes
T16297 450-454 VB denotes were
T16298 455-461 VB denotes washed
T16299 462-466 RB denotes once
T16300 467-469 IN denotes in
T16301 470-473 NN denotes PBS
T16302 474-484 VB denotes containing
T16303 485-486 CD denotes 1
T16304 486-487 NN denotes %
T16305 488-494 JJ denotes bovine
T16306 495-500 NN denotes serum
T16307 501-508 NN denotes albumin
T16308 509-510 -LRB- denotes (
T16309 510-513 NN denotes BSA
T16310 513-514 -RRB- denotes )
T16311 515-518 CC denotes and
T16312 519-531 JJ denotes re-suspended
T16313 532-534 IN denotes in
T16314 535-538 CD denotes 100
T16315 539-541 NN denotes µl
T16316 542-544 IN denotes of
T16317 545-552 NN denotes PBS/BSA
T16318 553-559 NN denotes buffer
T16319 561-570 RB denotes Meanwhile
T16320 570-571 -COMMA- denotes ,
T16321 572-575 CD denotes one
T16322 576-583 CD denotes million
T16323 584-589 NN denotes cells
T16324 590-598 VB denotes obtained
T16325 599-603 IN denotes from
T16326 604-609 NN denotes tumor
T16327 610-617 NN denotes tissues
T16328 618-622 VB denotes were
T16329 623-629 VB denotes placed
T16330 630-632 IN denotes in
T16331 633-637 NN denotes RPMI
T16332 638-644 NN denotes medium
T16333 644-645 -COMMA- denotes ,
T16334 646-655 VB denotes incubated
T16335 656-658 TO denotes to
T16336 659-665 VB denotes obtain
T16337 666-676 NN denotes suspension
T16338 677-681 NN denotes cell
T16339 682-685 IN denotes for
T16340 686-691 NN denotes 1hour
T16341 692-694 IN denotes at
T16342 695-699 NN denotes 37°C
T16343 699-700 -COMMA- denotes ,
T16344 701-712 VB denotes centrifuged
T16345 713-714 -LRB- denotes (
T16346 714-718 CD denotes 1200
T16347 719-722 NN denotes rpm
T16348 722-723 -COMMA- denotes ,
T16349 724-725 CD denotes 3
T16350 726-729 NN denotes min
T16351 729-730 -RRB- denotes )
T16352 731-734 CC denotes and
T16353 735-739 RB denotes then
T16354 740-746 VB denotes washed
T16355 747-749 IN denotes in
T16356 750-753 NNP denotes PBS
T16357 755-759 RB denotes Next
T16358 759-760 -COMMA- denotes ,
T16359 761-764 DT denotes the
T16360 765-776 NN denotes lymphocytes
T16361 777-781 VB denotes were
T16362 782-791 VB denotes incubated
T16363 792-796 IN denotes with
T16364 797-804 JJ denotes various
T16365 805-815 VB denotes conjugated
T16366 816-826 JJ denotes monoclonal
T16367 827-837 NN denotes antibodies
T16368 837-838 -COMMA- denotes ,
T16369 839-848 VB denotes including
T16370 849-856 NN denotes CD4-APC
T16371 856-857 -COMMA- denotes ,
T16372 858-866 NN denotes CD8-FITC
T16373 867-870 CC denotes and
T16374 871-878 NN denotes CD19-PE
T16375 879-882 IN denotes for
T16376 883-888 NN denotes 20min
T16377 889-891 IN denotes at
T16378 892-895 NN denotes 4°C
T16379 895-896 -COMMA- denotes ,
T16380 897-903 VB denotes washed
T16381 904-909 RB denotes twice
T16382 910-912 IN denotes in
T16383 913-916 NNP denotes PBS
T16384 916-917 -COMMA- denotes ,
T16385 918-921 CC denotes and
T16386 922-934 JJ denotes re-suspended
T16387 935-937 IN denotes in
T16388 938-941 CD denotes 400
T16389 942-944 NN denotes µl
T16390 945-947 IN denotes of
T16391 948-951 NNP denotes PBS
T16392 953-954 DT denotes A
T16393 955-959 NN denotes flow
T16394 960-970 JJ denotes cytometric
T16395 971-979 NN denotes analysis
T16396 980-983 VB denotes was
T16397 984-993 VB denotes performed
T16398 994-996 IN denotes on
T16399 997-998 DT denotes a
T16400 999-1007 NN denotes FACSAria
T16401 1008-1012 NN denotes flow
T16402 1013-1022 NN denotes cytometry
T16403 1023-1024 -LRB- denotes (
T16404 1024-1026 NN denotes BD
T16405 1027-1038 NNP denotes Biosciences
T16406 1038-1039 -RRB- denotes )
T16407 1039-1040 -COMMA- denotes ,
T16408 1041-1044 CC denotes and
T16409 1045-1048 DT denotes the
T16410 1049-1053 NN denotes data
T16411 1054-1058 VB denotes were
T16412 1059-1067 VB denotes analyzed
T16413 1068-1073 VB denotes using
T16414 1074-1077 DT denotes the
T16415 1078-1084 NNP denotes WinMDI
T16416 1085-1096 JJ denotes statistical
T16417 1097-1105 NN denotes software
T16418 1106-1107 -LRB- denotes (
T16419 1107-1114 NNP denotes Scripps
T16420 1114-1115 -COMMA- denotes ,
T16421 1116-1118 NNP denotes La
T16422 1119-1124 NNP denotes Jolla
T16423 1124-1125 -COMMA- denotes ,
T16424 1126-1128 NNP denotes CA
T16425 1128-1129 -COMMA- denotes ,
T16426 1130-1133 NNP denotes USA
T16427 1133-1134 -RRB- denotes )
T16428 1136-1143 RB denotes Forward
T16429 1144-1147 CC denotes and
T16430 1148-1152 NN denotes side
T16431 1153-1160 NN denotes scatter
T16432 1161-1171 NN denotes parameters
T16433 1172-1176 VB denotes were
T16434 1177-1181 VB denotes used
T16435 1182-1184 TO denotes to
T16436 1185-1189 VB denotes gate
T16437 1190-1192 IN denotes on
T16438 1193-1204 NN denotes lymphocytes
R11588 T16225 T16223 arg1Of Analysis,Flow
R11589 T16225 T16224 arg1Of Analysis,Cytometric
R11590 T16226 T16227 arg1Of Lymphocytes,were
R11591 T16226 T16228 arg2Of Lymphocytes,obtained
R11592 T16228 T16227 arg2Of obtained,were
R11593 T16228 T16229 arg1Of obtained,from
R11594 T16230 T16231 arg1Of tumor,and
R11595 T16232 T16231 arg2Of spleen,and
R11596 T16233 T16229 arg2Of tissues,from
R11597 T16233 T16230 arg1Of tissues,tumor
R11598 T16233 T16232 arg1Of tissues,spleen
R11599 T16233 T16234 arg1Of tissues,for
R11600 T16236 T16234 arg2Of analysis,for
R11601 T16236 T16235 arg1Of analysis,phenotype
R11602 T16237 T16238 arg1Of Spleen,and
R11603 T16239 T16238 arg2Of tumor,and
R11604 T16240 T16237 arg1Of lymphocytes,Spleen
R11605 T16240 T16239 arg1Of lymphocytes,tumor
R11606 T16240 T16241 arg1Of lymphocytes,were
R11607 T16240 T16242 arg2Of lymphocytes,isolated
R11608 T16242 T16241 arg2Of isolated,were
R11609 T16242 T16243 arg1Of isolated,from
R11610 T16245 T16246 arg1Of spleen,and
R11611 T16247 T16246 arg2Of tumor,and
R11612 T16248 T16243 arg2Of biopsies,from
R11613 T16248 T16244 arg1Of biopsies,fresh
R11614 T16248 T16245 arg1Of biopsies,spleen
R11615 T16248 T16247 arg1Of biopsies,tumor
R11616 T16248 T16249 arg2Of biopsies,obtained
R11617 T16249 T16250 arg1Of obtained,from
R11618 T16252 T16250 arg2Of mice,from
R11619 T16252 T16251 arg1Of mice,three
R11620 T16252 T16253 arg1Of mice,per
R11621 T16254 T16253 arg2Of group,per
R11622 T16255 T16256 arg1Of Spleen,and
R11623 T16257 T16256 arg2Of tumor,and
R11624 T16258 T16255 arg1Of biopsies,Spleen
R11625 T16258 T16257 arg1Of biopsies,tumor
R11626 T16258 T16259 arg1Of biopsies,were
R11627 T16258 T16261 arg2Of biopsies,homogenized
R11628 T16258 T16278 arg2Of biopsies,placed
R11629 T16261 T16262 arg1Of homogenized,mechanically
R11630 T16261 T16263 arg1Of homogenized,in
R11631 T16261 T16267 arg1Of homogenized,(
R11632 T16261 T16276 arg1Of homogenized,and
R11633 T16264 T16263 arg2Of PBS,in
R11634 T16264 T16265 arg1Of PBS,","
R11635 T16264 T16266 arg2Of PBS,filtered
R11636 T16271 T16268 arg1Of strainer,100
R11637 T16271 T16269 arg1Of strainer,µm
R11638 T16271 T16270 arg1Of strainer,cell
R11639 T16271 T16272 arg1Of strainer,;
R11640 T16272 T16267 arg2Of ;,(
R11641 T16274 T16272 arg2Of PharMingen,;
R11642 T16274 T16273 arg1Of PharMingen,BD
R11643 T16275 T16267 arg3Of ),(
R11644 T16276 T16259 arg2Of and,were
R11645 T16276 T16260 arg1Of and,briefly
R11646 T16278 T16276 arg2Of placed,and
R11647 T16278 T16277 arg1Of placed,then
R11648 T16278 T16279 arg1Of placed,in
R11649 T16278 T16289 modOf placed,to
R11650 T16281 T16279 arg2Of buffer,in
R11651 T16281 T16280 arg1Of buffer,lysis
R11652 T16281 T16282 arg1Of buffer,(
R11653 T16285 T16282 arg2Of buffer,(
R11654 T16285 T16283 arg1Of buffer,ACK
R11655 T16285 T16284 arg1Of buffer,lysing
R11656 T16285 T16286 arg1Of buffer,","
R11657 T16287 T16286 arg2Of LONZA,","
R11658 T16288 T16282 arg3Of ),(
R11659 T16290 T16289 arg1Of remove,to
R11660 T16293 T16290 arg2Of cells,remove
R11661 T16293 T16291 arg1Of cells,red
R11662 T16293 T16292 arg1Of cells,blood
R11663 T16295 T16294 arg1Of million,One
R11664 T16296 T16295 arg1Of splenocytes,million
R11665 T16296 T16297 arg1Of splenocytes,were
R11666 T16296 T16298 arg2Of splenocytes,washed
R11667 T16296 T16312 arg1Of splenocytes,re-suspended
R11668 T16298 T16299 arg1Of washed,once
R11669 T16298 T16300 arg1Of washed,in
R11670 T16298 T16311 arg1Of washed,and
R11671 T16301 T16300 arg2Of PBS,in
R11672 T16301 T16302 arg1Of PBS,containing
R11673 T16303 T16304 arg1Of 1,%
R11674 T16307 T16302 arg2Of albumin,containing
R11675 T16307 T16303 arg1Of albumin,1
R11676 T16307 T16305 arg1Of albumin,bovine
R11677 T16307 T16306 arg1Of albumin,serum
R11678 T16307 T16308 arg1Of albumin,(
R11679 T16309 T16308 arg2Of BSA,(
R11680 T16310 T16308 arg3Of ),(
R11681 T16311 T16297 arg2Of and,were
R11682 T16312 T16311 arg2Of re-suspended,and
R11683 T16312 T16313 arg1Of re-suspended,in
R11684 T16315 T16313 arg2Of µl,in
R11685 T16315 T16314 arg1Of µl,100
R11686 T16315 T16316 arg1Of µl,of
R11687 T16318 T16316 arg2Of buffer,of
R11688 T16318 T16317 arg1Of buffer,PBS/BSA
R11689 T16322 T16321 arg1Of million,one
R11690 T16323 T16322 arg1Of cells,million
R11691 T16323 T16324 arg2Of cells,obtained
R11692 T16323 T16328 arg1Of cells,were
R11693 T16323 T16329 arg2Of cells,placed
R11694 T16323 T16334 arg2Of cells,incubated
R11695 T16323 T16354 arg2Of cells,washed
R11696 T16324 T16325 arg1Of obtained,from
R11697 T16327 T16325 arg2Of tissues,from
R11698 T16327 T16326 arg1Of tissues,tumor
R11699 T16329 T16330 arg1Of placed,in
R11700 T16329 T16334 modOf placed,incubated
R11701 T16329 T16352 arg1Of placed,and
R11702 T16332 T16330 arg2Of medium,in
R11703 T16332 T16331 arg1Of medium,RPMI
R11704 T16334 T16333 arg1Of incubated,","
R11705 T16334 T16335 modOf incubated,to
R11706 T16336 T16335 arg1Of obtain,to
R11707 T16338 T16336 arg2Of cell,obtain
R11708 T16338 T16337 arg1Of cell,suspension
R11709 T16338 T16339 arg1Of cell,for
R11710 T16340 T16339 arg2Of 1hour,for
R11711 T16340 T16341 arg1Of 1hour,at
R11712 T16340 T16343 arg1Of 1hour,","
R11713 T16340 T16344 arg2Of 1hour,centrifuged
R11714 T16340 T16345 arg1Of 1hour,(
R11715 T16342 T16341 arg2Of 37°C,at
R11716 T16347 T16345 arg2Of rpm,(
R11717 T16347 T16346 arg1Of rpm,1200
R11718 T16347 T16348 arg1Of rpm,","
R11719 T16350 T16348 arg2Of min,","
R11720 T16350 T16349 arg1Of min,3
R11721 T16351 T16345 arg3Of ),(
R11722 T16352 T16319 arg1Of and,Meanwhile
R11723 T16352 T16320 arg1Of and,","
R11724 T16352 T16328 arg2Of and,were
R11725 T16354 T16352 arg2Of washed,and
R11726 T16354 T16353 arg1Of washed,then
R11727 T16354 T16355 arg1Of washed,in
R11728 T16356 T16355 arg2Of PBS,in
R11729 T16360 T16359 arg1Of lymphocytes,the
R11730 T16360 T16361 arg1Of lymphocytes,were
R11731 T16360 T16362 arg2Of lymphocytes,incubated
R11732 T16360 T16386 arg1Of lymphocytes,re-suspended
R11733 T16362 T16363 arg1Of incubated,with
R11734 T16362 T16385 arg1Of incubated,and
R11735 T16367 T16363 arg2Of antibodies,with
R11736 T16367 T16364 arg1Of antibodies,various
R11737 T16367 T16365 arg2Of antibodies,conjugated
R11738 T16367 T16366 arg1Of antibodies,monoclonal
R11739 T16367 T16368 arg1Of antibodies,","
R11740 T16367 T16369 arg1Of antibodies,including
R11741 T16370 T16371 arg1Of CD4-APC,","
R11742 T16371 T16373 arg1Of ",",and
R11743 T16372 T16371 arg2Of CD8-FITC,","
R11744 T16373 T16369 arg2Of and,including
R11745 T16373 T16375 arg1Of and,for
R11746 T16373 T16377 arg1Of and,at
R11747 T16373 T16379 arg1Of and,","
R11748 T16373 T16380 arg2Of and,washed
R11749 T16374 T16373 arg2Of CD19-PE,and
R11750 T16376 T16375 arg2Of 20min,for
R11751 T16378 T16377 arg2Of 4°C,at
R11752 T16380 T16381 arg1Of washed,twice
R11753 T16380 T16382 arg1Of washed,in
R11754 T16383 T16382 arg2Of PBS,in
R11755 T16385 T16357 arg1Of and,Next
R11756 T16385 T16358 arg1Of and,","
R11757 T16385 T16361 arg2Of and,were
R11758 T16385 T16384 arg1Of and,","
R11759 T16386 T16385 arg2Of re-suspended,and
R11760 T16386 T16387 arg1Of re-suspended,in
R11761 T16389 T16387 arg2Of µl,in
R11762 T16389 T16388 arg1Of µl,400
R11763 T16389 T16390 arg1Of µl,of
R11764 T16391 T16390 arg2Of PBS,of
R11765 T16395 T16392 arg1Of analysis,A
R11766 T16395 T16393 arg1Of analysis,flow
R11767 T16395 T16394 arg1Of analysis,cytometric
R11768 T16395 T16396 arg1Of analysis,was
R11769 T16395 T16397 arg2Of analysis,performed
R11770 T16397 T16396 arg2Of performed,was
R11771 T16397 T16398 arg1Of performed,on
R11772 T16397 T16408 arg1Of performed,and
R11773 T16402 T16398 arg2Of cytometry,on
R11774 T16402 T16399 arg1Of cytometry,a
R11775 T16402 T16400 arg1Of cytometry,FACSAria
R11776 T16402 T16401 arg1Of cytometry,flow
R11777 T16402 T16403 arg1Of cytometry,(
R11778 T16405 T16403 arg2Of Biosciences,(
R11779 T16405 T16404 arg1Of Biosciences,BD
R11780 T16406 T16403 arg3Of ),(
R11781 T16408 T16407 arg1Of and,","
R11782 T16410 T16409 arg1Of data,the
R11783 T16410 T16411 arg1Of data,were
R11784 T16410 T16412 arg2Of data,analyzed
R11785 T16412 T16408 arg2Of analyzed,and
R11786 T16412 T16411 arg2Of analyzed,were
R11787 T16413 T16412 arg3Of using,analyzed
R11788 T16413 T16418 arg1Of using,(
R11789 T16417 T16413 arg2Of software,using
R11790 T16417 T16414 arg1Of software,the
R11791 T16417 T16415 arg1Of software,WinMDI
R11792 T16417 T16416 arg1Of software,statistical
R11793 T16419 T16420 arg1Of Scripps,","
R11794 T16420 T16423 arg1Of ",",","
R11795 T16422 T16420 arg2Of Jolla,","
R11796 T16422 T16421 arg1Of Jolla,La
R11797 T16423 T16418 arg2Of ",",(
R11798 T16423 T16425 arg1Of ",",","
R11799 T16424 T16423 arg2Of CA,","
R11800 T16426 T16425 arg2Of USA,","
R11801 T16427 T16418 arg3Of ),(
R11802 T16432 T16428 arg1Of parameters,Forward
R11803 T16432 T16429 arg1Of parameters,and
R11804 T16432 T16430 arg1Of parameters,side
R11805 T16432 T16431 arg1Of parameters,scatter
R11806 T16432 T16433 arg1Of parameters,were
R11807 T16432 T16434 arg2Of parameters,used
R11808 T16434 T16433 arg2Of used,were
R11809 T16436 T16434 arg3Of gate,used
R11810 T16436 T16435 arg1Of gate,to
R11811 T16436 T16437 arg1Of gate,on
R11812 T16438 T16437 arg2Of lymphocytes,on