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PMC:3317373 / 9368-10539 JSONTXT 20 Projects

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Id Subject Object Predicate Lexical cue
T4081 0-3 CD denotes 2.7
T4082 5-18 JJ denotes Enzyme-Linked
T4083 19-32 JJ denotes Immunosorbent
T4084 33-38 NN denotes Assay
T4085 39-40 -LRB- denotes (
T4086 40-45 NN denotes ELISA
T4087 45-46 -RRB- denotes )
T4088 47-52 NN denotes Cells
T4089 53-55 IN denotes of
T4090 56-60 CD denotes four
T4091 61-67 NN denotes groups
T4092 68-72 VB denotes were
T4093 73-84 VB denotes homogenized
T4094 85-87 IN denotes as
T4095 88-97 VB denotes mentioned
T4096 98-103 IN denotes above
T4097 105-108 DT denotes The
T4098 109-120 NN denotes supernatant
T4099 121-124 VB denotes was
T4100 125-134 VB denotes collected
T4101 134-135 -COMMA- denotes ,
T4102 136-139 CC denotes and
T4103 140-145 JJ denotes total
T4104 146-153 NN denotes protein
T4105 154-157 VB denotes was
T4106 158-168 VB denotes determined
T4107 169-171 IN denotes by
T4108 172-181 NNP denotes Coomassie
T4109 182-186 NNP denotes Plus
T4110 187-194 NNP denotes Protein
T4111 195-200 NNP denotes Assay
T4112 201-208 NNP denotes Reagent
T4113 209-210 -LRB- denotes (
T4114 210-216 NNP denotes Pierce
T4115 216-217 -COMMA- denotes ,
T4116 218-220 NNP denotes IL
T4117 220-221 -COMMA- denotes ,
T4118 222-225 NNP denotes USA
T4119 225-226 -RRB- denotes )
T4120 228-234 NN denotes Levels
T4121 235-237 IN denotes of
T4122 238-243 NN denotes tumor
T4123 244-252 NN denotes necrosis
T4124 253-265 NN denotes factor-alpha
T4125 266-267 -LRB- denotes (
T4126 267-272 NN denotes TNF-α
T4127 272-273 -RRB- denotes )
T4128 273-274 -COMMA- denotes ,
T4129 275-288 NN denotes interleukin-1
T4130 289-293 NN denotes beta
T4131 294-295 -LRB- denotes (
T4132 295-300 NN denotes IL-1β
T4133 300-301 -RRB- denotes )
T4134 301-302 -COMMA- denotes ,
T4135 303-306 CC denotes and
T4136 307-320 NN denotes interleukin-6
T4137 321-322 -LRB- denotes (
T4138 322-326 NN denotes IL-6
T4139 326-327 -RRB- denotes )
T4140 328-335 NN denotes protein
T4141 336-340 VB denotes were
T4142 341-351 VB denotes quantified
T4143 352-357 VB denotes using
T4144 358-363 NN denotes ELISA
T4145 364-368 NN denotes kits
T4146 369-377 JJ denotes specific
T4147 378-381 IN denotes for
T4148 382-387 NN denotes mouse
T4149 388-397 VB denotes according
T4150 398-400 TO denotes to
T4151 401-404 DT denotes the
T4152 405-417 NN denotes manufacturer
T4153 417-419 POS denotes 's
T4154 420-432 NN denotes instructions
T4155 433-434 -LRB- denotes (
T4156 434-440 NNP denotes Bender
T4157 441-451 NNP denotes MedSystems
T4158 452-456 NNP denotes Inc.
T4159 457-459 NNP denotes CA
T4160 459-460 -COMMA- denotes ,
T4161 461-464 NNP denotes USA
T4162 464-465 -RRB- denotes )
T4163 467-474 NNP denotes Briefly
T4164 474-475 -COMMA- denotes ,
T4165 476-484 VB denotes prepared
T4166 485-488 DT denotes the
T4167 489-497 NN denotes standard
T4168 498-501 CC denotes and
T4169 502-509 VB denotes created
T4170 510-513 DT denotes the
T4171 514-522 JJ denotes standard
T4172 523-531 NN denotes dilution
T4173 532-535 IN denotes for
T4174 536-544 VB denotes building
T4175 545-553 JJ denotes standard
T4176 554-559 NN denotes curve
T4177 561-565 RB denotes Then
T4178 566-573 NN denotes samples
T4179 574-577 CC denotes and
T4180 578-593 NN denotes biotinconjugate
T4181 594-598 VB denotes were
T4182 599-604 VB denotes added
T4183 605-607 TO denotes to
T4184 608-617 VB denotes microwell
T4185 618-624 NN denotes strips
T4186 626-631 IN denotes After
T4187 632-641 VB denotes incubated
T4188 642-645 IN denotes for
T4189 646-647 CD denotes 2
T4190 648-649 NN denotes h
T4191 650-652 IN denotes at
T4192 653-657 NN denotes room
T4193 658-669 NN denotes temperature
T4194 669-670 -COMMA- denotes ,
T4195 671-674 DT denotes the
T4196 675-684 NN denotes microwell
T4197 685-691 NN denotes strips
T4198 692-696 VB denotes were
T4199 697-703 VB denotes washed
T4200 704-705 CD denotes 3
T4201 706-711 NN denotes times
T4202 712-716 IN denotes with
T4203 717-721 NN denotes wash
T4204 722-728 NN denotes buffer
T4205 728-729 -COMMA- denotes ,
T4206 730-733 CC denotes and
T4207 734-750 NN denotes streptavidin-HRP
T4208 751-755 VB denotes were
T4209 756-761 VB denotes added
T4210 762-764 TO denotes to
T4211 765-768 DT denotes all
T4212 769-774 NN denotes wells
T4213 776-781 IN denotes After
T4214 782-791 VB denotes incubated
T4215 792-795 IN denotes for
T4216 796-797 CD denotes 1
T4217 798-799 NN denotes h
T4218 799-800 -COMMA- denotes ,
T4219 801-810 NN denotes microwell
T4220 811-817 NN denotes strips
T4221 818-822 VB denotes were
T4222 823-829 VB denotes washed
T4223 830-831 CD denotes 3
T4224 832-837 NN denotes times
T4225 838-846 VB denotes followed
T4226 847-849 IN denotes by
T4227 850-856 VB denotes adding
T4228 857-860 NN denotes TMB
T4229 861-870 NN denotes substrate
T4230 872-877 IN denotes After
T4231 878-887 VB denotes incubated
T4232 888-891 IN denotes for
T4233 892-897 RB denotes about
T4234 898-903 CD denotes 10–30
T4235 904-907 NN denotes min
T4236 907-908 -COMMA- denotes ,
T4237 909-912 DT denotes the
T4238 913-917 NN denotes stop
T4239 918-926 NN denotes solution
T4240 927-930 VB denotes was
T4241 931-936 VB denotes added
T4242 938-941 DT denotes The
T4243 942-948 NN denotes colour
T4244 949-958 NN denotes intensity
T4245 959-962 VB denotes was
T4246 963-971 VB denotes measured
T4247 972-974 IN denotes at
T4248 975-978 CD denotes 450
T4249 979-981 NN denotes nm
T4250 982-987 VB denotes using
T4251 988-989 DT denotes a
T4252 990-997 JJ denotes Bio-Rad
T4253 998-1003 NN denotes ELISA
T4254 1004-1014 NN denotes microplate
T4255 1015-1021 NN denotes reader
T4256 1022-1023 -LRB- denotes (
T4257 1023-1030 NNP denotes Bio-Rad
T4258 1031-1043 NNP denotes Laboratories
T4259 1043-1044 -COMMA- denotes ,
T4260 1045-1047 NNP denotes CA
T4261 1047-1048 -COMMA- denotes ,
T4262 1049-1052 NNP denotes USA
T4263 1052-1053 -RRB- denotes )
T4264 1055-1058 DT denotes The
T4265 1059-1072 NN denotes concentration
T4266 1073-1075 IN denotes of
T4267 1076-1083 NN denotes protein
T4268 1084-1087 VB denotes was
T4269 1088-1098 VB denotes determined
T4270 1099-1108 VB denotes according
T4271 1109-1111 TO denotes to
T4272 1112-1115 DT denotes the
T4273 1116-1124 JJ denotes standard
T4274 1125-1130 NN denotes curve
T4275 1131-1134 CC denotes and
T4276 1135-1144 VB denotes expressed
T4277 1145-1147 IN denotes as
T4278 1148-1153 NN denotes pg/mg
T4279 1154-1156 IN denotes of
T4280 1157-1162 JJ denotes total
T4281 1163-1170 NN denotes protein
R3492 T4084 T4082 arg1Of Assay,Enzyme-Linked
R3493 T4084 T4083 arg1Of Assay,Immunosorbent
R3494 T4084 T4085 arg1Of Assay,(
R3495 T4086 T4085 arg2Of ELISA,(
R3496 T4087 T4085 arg3Of ),(
R3497 T4088 T4089 arg1Of Cells,of
R3498 T4088 T4092 arg1Of Cells,were
R3499 T4088 T4093 arg2Of Cells,homogenized
R3500 T4091 T4089 arg2Of groups,of
R3501 T4091 T4090 arg1Of groups,four
R3502 T4093 T4092 arg2Of homogenized,were
R3503 T4093 T4094 arg1Of homogenized,as
R3504 T4095 T4094 arg2Of mentioned,as
R3505 T4095 T4096 arg1Of mentioned,above
R3506 T4098 T4097 arg1Of supernatant,The
R3507 T4098 T4099 arg1Of supernatant,was
R3508 T4098 T4100 arg2Of supernatant,collected
R3509 T4100 T4099 arg2Of collected,was
R3510 T4100 T4102 arg1Of collected,and
R3511 T4102 T4101 arg1Of and,","
R3512 T4104 T4103 arg1Of protein,total
R3513 T4104 T4105 arg1Of protein,was
R3514 T4104 T4106 arg2Of protein,determined
R3515 T4106 T4102 arg2Of determined,and
R3516 T4106 T4105 arg2Of determined,was
R3517 T4112 T4106 arg1Of Reagent,determined
R3518 T4112 T4107 arg2Of Reagent,by
R3519 T4112 T4108 arg1Of Reagent,Coomassie
R3520 T4112 T4109 arg1Of Reagent,Plus
R3521 T4112 T4110 arg1Of Reagent,Protein
R3522 T4112 T4111 arg1Of Reagent,Assay
R3523 T4112 T4113 arg1Of Reagent,(
R3524 T4114 T4115 arg1Of Pierce,","
R3525 T4115 T4113 arg2Of ",",(
R3526 T4115 T4117 arg1Of ",",","
R3527 T4116 T4115 arg2Of IL,","
R3528 T4118 T4117 arg2Of USA,","
R3529 T4119 T4113 arg3Of ),(
R3530 T4120 T4121 arg1Of Levels,of
R3531 T4120 T4135 arg1Of Levels,and
R3532 T4124 T4122 arg1Of factor-alpha,tumor
R3533 T4124 T4123 arg1Of factor-alpha,necrosis
R3534 T4124 T4125 arg1Of factor-alpha,(
R3535 T4124 T4128 arg1Of factor-alpha,","
R3536 T4126 T4125 arg2Of TNF-α,(
R3537 T4127 T4125 arg3Of ),(
R3538 T4128 T4121 arg2Of ",",of
R3539 T4130 T4128 arg2Of beta,","
R3540 T4130 T4129 arg1Of beta,interleukin-1
R3541 T4130 T4131 arg1Of beta,(
R3542 T4132 T4131 arg2Of IL-1β,(
R3543 T4133 T4131 arg3Of ),(
R3544 T4135 T4134 arg1Of and,","
R3545 T4136 T4135 arg2Of interleukin-6,and
R3546 T4136 T4137 arg1Of interleukin-6,(
R3547 T4136 T4141 modOf interleukin-6,were
R3548 T4138 T4137 arg2Of IL-6,(
R3549 T4139 T4137 arg3Of ),(
R3550 T4140 T4141 arg1Of protein,were
R3551 T4140 T4142 arg2Of protein,quantified
R3552 T4142 T4141 arg2Of quantified,were
R3553 T4143 T4142 arg3Of using,quantified
R3554 T4143 T4149 arg1Of using,according
R3555 T4145 T4143 arg2Of kits,using
R3556 T4145 T4144 arg1Of kits,ELISA
R3557 T4145 T4146 arg1Of kits,specific
R3558 T4146 T4147 arg1Of specific,for
R3559 T4148 T4147 arg2Of mouse,for
R3560 T4150 T4149 arg2Of to,according
R3561 T4152 T4151 arg1Of manufacturer,the
R3562 T4152 T4153 arg2Of manufacturer,'s
R3563 T4154 T4150 arg2Of instructions,to
R3564 T4154 T4153 arg1Of instructions,'s
R3565 T4154 T4155 arg1Of instructions,(
R3566 T4159 T4155 arg2Of CA,(
R3567 T4159 T4156 arg1Of CA,Bender
R3568 T4159 T4157 arg1Of CA,MedSystems
R3569 T4159 T4158 arg1Of CA,Inc.
R3570 T4159 T4160 arg1Of CA,","
R3571 T4159 T4161 arg1Of CA,USA
R3572 T4162 T4155 arg3Of ),(
R3573 T4163 T4165 arg1Of Briefly,prepared
R3574 T4163 T4169 arg1Of Briefly,created
R3575 T4165 T4168 arg1Of prepared,and
R3576 T4167 T4165 arg2Of standard,prepared
R3577 T4167 T4166 arg1Of standard,the
R3578 T4168 T4164 arg1Of and,","
R3579 T4169 T4168 arg2Of created,and
R3580 T4172 T4169 arg2Of dilution,created
R3581 T4172 T4170 arg1Of dilution,the
R3582 T4172 T4171 arg1Of dilution,standard
R3583 T4172 T4173 arg1Of dilution,for
R3584 T4174 T4173 arg2Of building,for
R3585 T4176 T4174 arg2Of curve,building
R3586 T4176 T4175 arg1Of curve,standard
R3587 T4178 T4179 arg1Of samples,and
R3588 T4179 T4177 arg1Of and,Then
R3589 T4179 T4181 arg1Of and,were
R3590 T4179 T4182 arg2Of and,added
R3591 T4180 T4179 arg2Of biotinconjugate,and
R3592 T4182 T4181 arg2Of added,were
R3593 T4182 T4183 modOf added,to
R3594 T4184 T4183 arg1Of microwell,to
R3595 T4185 T4184 arg2Of strips,microwell
R3596 T4187 T4188 arg1Of incubated,for
R3597 T4190 T4188 arg2Of h,for
R3598 T4190 T4189 arg1Of h,2
R3599 T4190 T4191 arg1Of h,at
R3600 T4192 T4186 arg1Of room,After
R3601 T4192 T4187 arg2Of room,incubated
R3602 T4193 T4192 arg1Of temperature,room
R3603 T4193 T4194 arg1Of temperature,","
R3604 T4193 T4198 arg1Of temperature,were
R3605 T4193 T4199 arg2Of temperature,washed
R3606 T4197 T4194 arg2Of strips,","
R3607 T4197 T4195 arg1Of strips,the
R3608 T4197 T4196 arg1Of strips,microwell
R3609 T4199 T4198 arg2Of washed,were
R3610 T4199 T4202 arg1Of washed,with
R3611 T4199 T4206 arg1Of washed,and
R3612 T4201 T4199 arg3Of times,washed
R3613 T4201 T4200 arg1Of times,3
R3614 T4204 T4202 arg2Of buffer,with
R3615 T4204 T4203 arg1Of buffer,wash
R3616 T4206 T4205 arg1Of and,","
R3617 T4207 T4208 arg1Of streptavidin-HRP,were
R3618 T4207 T4209 arg2Of streptavidin-HRP,added
R3619 T4209 T4206 arg2Of added,and
R3620 T4209 T4208 arg2Of added,were
R3621 T4209 T4210 arg1Of added,to
R3622 T4212 T4210 arg2Of wells,to
R3623 T4212 T4211 arg1Of wells,all
R3624 T4214 T4215 arg1Of incubated,for
R3625 T4217 T4215 arg2Of h,for
R3626 T4217 T4216 arg1Of h,1
R3627 T4220 T4213 arg1Of strips,After
R3628 T4220 T4214 arg2Of strips,incubated
R3629 T4220 T4218 arg1Of strips,","
R3630 T4220 T4219 arg1Of strips,microwell
R3631 T4220 T4221 arg1Of strips,were
R3632 T4220 T4222 arg2Of strips,washed
R3633 T4222 T4221 arg2Of washed,were
R3634 T4224 T4222 arg3Of times,washed
R3635 T4224 T4223 arg1Of times,3
R3636 T4224 T4225 arg2Of times,followed
R3637 T4225 T4226 arg1Of followed,by
R3638 T4227 T4226 arg2Of adding,by
R3639 T4229 T4227 arg2Of substrate,adding
R3640 T4229 T4228 arg1Of substrate,TMB
R3641 T4231 T4232 arg1Of incubated,for
R3642 T4234 T4230 arg1Of 10–30,After
R3643 T4234 T4231 arg2Of 10–30,incubated
R3644 T4234 T4233 arg1Of 10–30,about
R3645 T4235 T4234 arg1Of min,10–30
R3646 T4235 T4236 arg1Of min,","
R3647 T4235 T4240 arg1Of min,was
R3648 T4235 T4241 arg2Of min,added
R3649 T4239 T4236 arg2Of solution,","
R3650 T4239 T4237 arg1Of solution,the
R3651 T4239 T4238 arg1Of solution,stop
R3652 T4241 T4240 arg2Of added,was
R3653 T4244 T4242 arg1Of intensity,The
R3654 T4244 T4243 arg1Of intensity,colour
R3655 T4244 T4245 arg1Of intensity,was
R3656 T4244 T4246 arg2Of intensity,measured
R3657 T4244 T4250 arg1Of intensity,using
R3658 T4246 T4245 arg2Of measured,was
R3659 T4246 T4247 arg1Of measured,at
R3660 T4246 T4250 modOf measured,using
R3661 T4249 T4247 arg2Of nm,at
R3662 T4249 T4248 arg1Of nm,450
R3663 T4250 T4256 arg1Of using,(
R3664 T4255 T4250 arg2Of reader,using
R3665 T4255 T4251 arg1Of reader,a
R3666 T4255 T4252 arg1Of reader,Bio-Rad
R3667 T4255 T4253 arg1Of reader,ELISA
R3668 T4255 T4254 arg1Of reader,microplate
R3669 T4258 T4256 arg2Of Laboratories,(
R3670 T4258 T4257 arg1Of Laboratories,Bio-Rad
R3671 T4258 T4259 arg1Of Laboratories,","
R3672 T4260 T4259 arg2Of CA,","
R3673 T4260 T4261 arg1Of CA,","
R3674 T4262 T4261 arg2Of USA,","
R3675 T4263 T4256 arg3Of ),(
R3676 T4265 T4264 arg1Of concentration,The
R3677 T4265 T4266 arg1Of concentration,of
R3678 T4265 T4268 arg1Of concentration,was
R3679 T4265 T4269 arg2Of concentration,determined
R3680 T4265 T4276 arg2Of concentration,expressed
R3681 T4267 T4266 arg2Of protein,of
R3682 T4269 T4270 arg1Of determined,according
R3683 T4269 T4275 arg1Of determined,and
R3684 T4271 T4270 arg2Of to,according
R3685 T4274 T4271 arg2Of curve,to
R3686 T4274 T4272 arg1Of curve,the
R3687 T4274 T4273 arg1Of curve,standard
R3688 T4275 T4268 arg2Of and,was
R3689 T4276 T4275 arg2Of expressed,and
R3690 T4276 T4277 arg1Of expressed,as
R3691 T4278 T4277 arg2Of pg/mg,as
R3692 T4278 T4279 arg1Of pg/mg,of
R3693 T4281 T4279 arg2Of protein,of
R3694 T4281 T4280 arg1Of protein,total