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PMC:3317373 / 7259-7858 JSONTXT 21 Projects

Annnotations TAB TSV DIC JSON TextAE

Id Subject Object Predicate Lexical cue
T3115 0-3 CD denotes 2.5
T3116 5-11 NN denotes RT-PCR
T3117 12-17 JJ denotes Total
T3118 18-21 NN denotes RNA
T3119 22-25 VB denotes was
T3120 26-34 VB denotes isolated
T3121 35-39 IN denotes with
T3122 40-46 NN denotes Trizol
T3123 47-48 -LRB- denotes (
T3124 48-58 NN denotes Invitrogen
T3125 58-59 -COMMA- denotes ,
T3126 60-62 NNP denotes CA
T3127 62-63 -COMMA- denotes ,
T3128 64-67 NNP denotes USA
T3129 67-68 -RRB- denotes )
T3130 68-69 -COMMA- denotes ,
T3131 70-73 CC denotes and
T3132 74-89 JJ denotes single-stranded
T3133 90-94 NN denotes cDNA
T3134 95-98 VB denotes was
T3135 99-110 VB denotes synthesized
T3136 111-115 IN denotes from
T3137 116-117 CD denotes 2
T3138 118-120 NN denotes μg
T3139 121-123 IN denotes of
T3140 124-129 JJ denotes total
T3141 130-133 NN denotes RNA
T3142 134-138 IN denotes with
T3143 139-148 JJ denotes BU-Script
T3144 149-155 NN denotes RT-Kit
T3145 156-157 -LRB- denotes (
T3146 157-165 NNP denotes Biunique
T3147 165-166 -COMMA- denotes ,
T3148 167-174 NNP denotes Jiangsu
T3149 174-175 -COMMA- denotes ,
T3150 176-181 NNP denotes China
T3151 181-182 -RRB- denotes )
T3152 183-192 VB denotes according
T3153 193-195 TO denotes to
T3154 196-199 DT denotes the
T3155 200-212 NN denotes manufacturer
T3156 212-214 POS denotes 's
T3157 215-223 NN denotes protocol
T3158 225-228 DT denotes The
T3159 229-233 NN denotes cDNA
T3160 234-237 VB denotes was
T3161 238-244 VB denotes stored
T3162 245-247 IN denotes in
T3163 248-253 NN denotes −20°C
T3164 255-262 JJ denotes Reverse
T3165 263-276 NN denotes transcription
T3166 277-280 VB denotes was
T3167 281-290 VB denotes conducted
T3168 291-295 IN denotes with
T3169 296-301 NNP denotes GoTaq
T3170 302-307 NNP denotes Green
T3171 308-314 NNP denotes Master
T3172 315-318 NNP denotes Mix
T3173 319-320 -LRB- denotes (
T3174 320-327 NNP denotes Promega
T3175 327-328 -COMMA- denotes ,
T3176 329-331 NNP denotes WI
T3177 331-332 -COMMA- denotes ,
T3178 333-336 NNP denotes USA
T3179 336-337 -RRB- denotes )
T3180 338-347 VB denotes according
T3181 348-350 TO denotes to
T3182 351-354 DT denotes the
T3183 355-367 NN denotes manufacturer
T3184 367-369 POS denotes 's
T3185 370-378 NN denotes protocol
T3186 381-386 JJ denotes Table
T3187 387-388 CD denotes 1
T3188 389-394 VB denotes shows
T3189 395-398 DT denotes the
T3190 399-406 NN denotes primers
T3191 407-410 CC denotes and
T3192 411-414 NN denotes PCR
T3193 415-425 NN denotes parameters
T3194 427-430 NN denotes PCR
T3195 431-439 NN denotes products
T3196 440-444 VB denotes were
T3197 445-453 VB denotes detected
T3198 454-456 IN denotes by
T3199 457-464 NN denotes agarose
T3200 465-468 NN denotes gel
T3201 469-484 NN denotes electrophoresis
T3202 486-489 DT denotes The
T3203 490-499 NN denotes intensity
T3204 500-502 IN denotes of
T3205 503-506 DT denotes the
T3206 507-512 NN denotes bands
T3207 513-516 VB denotes was
T3208 517-525 VB denotes analyzed
T3209 526-528 IN denotes by
T3210 529-535 NN denotes ImageJ
T3211 536-543 NN denotes program
T3212 545-548 DT denotes The
T3213 549-554 NN denotes level
T3214 555-557 IN denotes of
T3215 558-565 NN denotes β-actin
T3216 566-569 VB denotes was
T3217 570-574 VB denotes used
T3218 575-577 IN denotes as
T3219 578-580 DT denotes an
T3220 581-589 JJ denotes internal
T3221 590-598 NN denotes standard
R2677 T3118 T3117 arg1Of RNA,Total
R2678 T3118 T3119 arg1Of RNA,was
R2679 T3118 T3120 arg2Of RNA,isolated
R2680 T3120 T3119 arg2Of isolated,was
R2681 T3120 T3121 arg1Of isolated,with
R2682 T3120 T3131 arg1Of isolated,and
R2683 T3122 T3121 arg2Of Trizol,with
R2684 T3122 T3123 arg1Of Trizol,(
R2685 T3124 T3123 arg2Of Invitrogen,(
R2686 T3124 T3125 arg1Of Invitrogen,","
R2687 T3124 T3127 arg1Of Invitrogen,","
R2688 T3124 T3128 arg1Of Invitrogen,USA
R2689 T3126 T3125 arg2Of CA,","
R2690 T3129 T3123 arg3Of ),(
R2691 T3131 T3130 arg1Of and,","
R2692 T3131 T3145 arg1Of and,(
R2693 T3131 T3152 arg1Of and,according
R2694 T3133 T3132 arg1Of cDNA,single-stranded
R2695 T3133 T3134 arg1Of cDNA,was
R2696 T3133 T3135 arg2Of cDNA,synthesized
R2697 T3135 T3131 arg2Of synthesized,and
R2698 T3135 T3134 arg2Of synthesized,was
R2699 T3135 T3136 arg1Of synthesized,from
R2700 T3138 T3136 arg2Of μg,from
R2701 T3138 T3137 arg1Of μg,2
R2702 T3138 T3139 arg1Of μg,of
R2703 T3141 T3139 arg2Of RNA,of
R2704 T3141 T3140 arg1Of RNA,total
R2705 T3141 T3142 arg1Of RNA,with
R2706 T3144 T3142 arg2Of RT-Kit,with
R2707 T3144 T3143 arg1Of RT-Kit,BU-Script
R2708 T3146 T3147 arg1Of Biunique,","
R2709 T3147 T3145 arg2Of ",",(
R2710 T3147 T3149 arg1Of ",",","
R2711 T3148 T3147 arg2Of Jiangsu,","
R2712 T3150 T3149 arg2Of China,","
R2713 T3151 T3145 arg3Of ),(
R2714 T3153 T3152 arg2Of to,according
R2715 T3155 T3154 arg1Of manufacturer,the
R2716 T3155 T3156 arg2Of manufacturer,'s
R2717 T3157 T3153 arg2Of protocol,to
R2718 T3157 T3156 arg1Of protocol,'s
R2719 T3159 T3158 arg1Of cDNA,The
R2720 T3159 T3160 arg1Of cDNA,was
R2721 T3159 T3161 arg2Of cDNA,stored
R2722 T3161 T3160 arg2Of stored,was
R2723 T3161 T3162 arg1Of stored,in
R2724 T3163 T3162 arg2Of −20°C,in
R2725 T3165 T3164 arg1Of transcription,Reverse
R2726 T3165 T3166 arg1Of transcription,was
R2727 T3165 T3167 arg2Of transcription,conducted
R2728 T3167 T3166 arg2Of conducted,was
R2729 T3167 T3168 arg1Of conducted,with
R2730 T3167 T3180 arg1Of conducted,according
R2731 T3172 T3168 arg2Of Mix,with
R2732 T3172 T3169 arg1Of Mix,GoTaq
R2733 T3172 T3170 arg1Of Mix,Green
R2734 T3172 T3171 arg1Of Mix,Master
R2735 T3172 T3173 arg1Of Mix,(
R2736 T3174 T3175 arg1Of Promega,","
R2737 T3175 T3173 arg2Of ",",(
R2738 T3175 T3177 arg1Of ",",","
R2739 T3175 T3178 arg1Of ",",USA
R2740 T3176 T3175 arg2Of WI,","
R2741 T3179 T3173 arg3Of ),(
R2742 T3181 T3180 arg2Of to,according
R2743 T3183 T3182 arg1Of manufacturer,the
R2744 T3183 T3184 arg2Of manufacturer,'s
R2745 T3185 T3181 arg2Of protocol,to
R2746 T3185 T3184 arg1Of protocol,'s
R2747 T3187 T3186 arg1Of 1,Table
R2748 T3187 T3188 arg1Of 1,shows
R2749 T3190 T3191 arg1Of primers,and
R2750 T3191 T3188 arg2Of and,shows
R2751 T3191 T3189 arg1Of and,the
R2752 T3193 T3191 arg2Of parameters,and
R2753 T3193 T3192 arg1Of parameters,PCR
R2754 T3195 T3194 arg1Of products,PCR
R2755 T3195 T3196 arg1Of products,were
R2756 T3195 T3197 arg2Of products,detected
R2757 T3197 T3196 arg2Of detected,were
R2758 T3201 T3197 arg1Of electrophoresis,detected
R2759 T3201 T3198 arg2Of electrophoresis,by
R2760 T3201 T3199 arg1Of electrophoresis,agarose
R2761 T3201 T3200 arg1Of electrophoresis,gel
R2762 T3203 T3202 arg1Of intensity,The
R2763 T3203 T3204 arg1Of intensity,of
R2764 T3203 T3207 arg1Of intensity,was
R2765 T3203 T3208 arg2Of intensity,analyzed
R2766 T3206 T3204 arg2Of bands,of
R2767 T3206 T3205 arg1Of bands,the
R2768 T3208 T3207 arg2Of analyzed,was
R2769 T3211 T3208 arg1Of program,analyzed
R2770 T3211 T3209 arg2Of program,by
R2771 T3211 T3210 arg1Of program,ImageJ
R2772 T3213 T3212 arg1Of level,The
R2773 T3213 T3214 arg1Of level,of
R2774 T3213 T3216 arg1Of level,was
R2775 T3213 T3217 arg2Of level,used
R2776 T3215 T3214 arg2Of β-actin,of
R2777 T3217 T3216 arg2Of used,was
R2778 T3217 T3218 arg1Of used,as
R2779 T3221 T3218 arg2Of standard,as
R2780 T3221 T3219 arg1Of standard,an
R2781 T3221 T3220 arg1Of standard,internal