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PMC:3317373 / 5505-7257 JSONTXT 9 Projects

Annnotations TAB TSV DIC JSON TextAE

Id Subject Object Predicate Lexical cue
T2331 0-10 NN denotes Monolayers
T2332 11-13 IN denotes of
T2333 14-24 NN denotes astrocytes
T2334 25-29 VB denotes were
T2335 30-36 VB denotes washed
T2336 37-41 IN denotes with
T2337 42-45 NNP denotes PBS
T2338 46-49 CC denotes and
T2339 50-59 VB denotes harvested
T2340 60-62 IN denotes by
T2341 63-71 VB denotes scraping
T2342 72-76 IN denotes into
T2343 77-81 JJ denotes cold
T2344 82-85 NNP denotes PBS
T2345 87-90 DT denotes The
T2346 91-95 NN denotes cell
T2347 96-102 NN denotes pellet
T2348 103-111 VB denotes obtained
T2349 112-114 IN denotes by
T2350 115-129 NN denotes centrifugation
T2351 130-133 VB denotes was
T2352 134-145 VB denotes resuspended
T2353 146-148 IN denotes in
T2354 149-155 NN denotes buffer
T2355 156-166 VB denotes containing
T2356 167-169 CD denotes 10
T2357 170-172 NN denotes mM
T2358 173-178 NN denotes HEPES
T2359 179-180 -LRB- denotes (
T2360 180-182 NN denotes pH
T2361 183-186 CD denotes 7.9
T2362 186-187 -RRB- denotes )
T2363 187-188 -COMMA- denotes ,
T2364 189-191 CD denotes 10
T2365 192-194 NN denotes mM
T2366 195-198 NN denotes KCl
T2367 198-199 -COMMA- denotes ,
T2368 200-203 CD denotes 0.1
T2369 204-206 NN denotes mM
T2370 207-211 NN denotes EDTA
T2371 211-212 -COMMA- denotes ,
T2372 213-216 CD denotes 0.1
T2373 217-219 NN denotes mM
T2374 220-224 NN denotes EGTA
T2375 224-225 -COMMA- denotes ,
T2376 226-229 CD denotes 1.0
T2377 230-232 NN denotes mM
T2378 233-236 NN denotes DTT
T2379 236-237 -COMMA- denotes ,
T2380 238-241 CC denotes and
T2381 242-245 CD denotes 0.5
T2382 246-248 NN denotes mM
T2383 249-269 NN denotes phenylmethylsulfonyl
T2384 270-278 NN denotes fluoride
T2385 280-284 RB denotes Then
T2386 285-287 CD denotes 10
T2387 287-288 NN denotes %
T2388 289-296 NN denotes Nonidet
T2389 297-301 NN denotes P-40
T2390 302-305 VB denotes was
T2391 306-311 VB denotes added
T2392 312-315 CC denotes and
T2393 316-324 VB denotes vortexed
T2394 325-332 NN denotes briefly
T2395 332-333 -COMMA- denotes ,
T2396 334-337 CC denotes and
T2397 338-341 DT denotes the
T2398 342-348 NN denotes nuclei
T2399 349-353 VB denotes were
T2400 354-362 VB denotes pelleted
T2401 363-365 IN denotes by
T2402 366-380 NN denotes centrifugation
T2403 382-385 DT denotes The
T2404 386-393 JJ denotes nuclear
T2405 394-402 NN denotes proteins
T2406 403-407 VB denotes were
T2407 408-417 VB denotes extracted
T2408 418-422 IN denotes with
T2409 423-429 NN denotes buffer
T2410 430-440 VB denotes containing
T2411 441-443 CD denotes 20
T2412 444-446 NN denotes mM
T2413 447-452 NN denotes HEPES
T2414 453-454 -LRB- denotes (
T2415 454-456 NN denotes pH
T2416 457-460 CD denotes 7.9
T2417 460-461 -RRB- denotes )
T2418 461-462 -COMMA- denotes ,
T2419 463-466 CD denotes 0.4
T2420 467-469 NN denotes mM
T2421 470-474 NN denotes NaCl
T2422 474-475 -COMMA- denotes ,
T2423 476-479 CD denotes 1.0
T2424 480-482 NN denotes mM
T2425 483-487 NN denotes EDTA
T2426 487-488 -COMMA- denotes ,
T2427 489-492 CD denotes 1.0
T2428 493-495 NN denotes mM
T2429 496-500 NN denotes EGTA
T2430 500-501 -COMMA- denotes ,
T2431 502-505 CD denotes 1.0
T2432 506-508 NN denotes mM
T2433 509-512 NN denotes DTT
T2434 512-513 -COMMA- denotes ,
T2435 514-517 CC denotes and
T2436 518-521 CD denotes 1.0
T2437 522-524 NN denotes mM
T2438 525-545 NN denotes phenylmethylsulfonyl
T2439 546-554 NN denotes fluoride
T2440 556-565 JJ denotes Insoluble
T2441 566-574 NN denotes material
T2442 575-578 VB denotes was
T2443 579-586 VB denotes removed
T2444 587-589 IN denotes by
T2445 590-604 NN denotes centrifugation
T2446 605-607 IN denotes at
T2447 608-613 CD denotes 14000
T2448 614-617 NN denotes rpm
T2449 617-618 -COMMA- denotes ,
T2450 619-622 CC denotes and
T2451 623-626 DT denotes the
T2452 627-638 NN denotes supernatant
T2453 639-649 VB denotes containing
T2454 650-653 DT denotes the
T2455 654-661 JJ denotes nuclear
T2456 662-670 NN denotes proteins
T2457 671-674 VB denotes was
T2458 675-681 VB denotes stored
T2459 682-684 IN denotes at
T2460 685-690 NN denotes –80°C
T2461 691-696 IN denotes until
T2462 697-700 NN denotes use
T2463 702-709 NN denotes Protein
T2464 710-723 NN denotes concentration
T2465 724-727 VB denotes was
T2466 728-738 VB denotes determined
T2467 739-744 VB denotes using
T2468 745-746 DT denotes a
T2469 747-760 JJ denotes bicinchoninic
T2470 761-765 NN denotes acid
T2471 766-771 NN denotes assay
T2472 772-775 NN denotes kit
T2473 776-780 IN denotes with
T2474 781-787 JJ denotes bovine
T2475 788-793 NN denotes serum
T2476 794-801 NN denotes albumin
T2477 802-804 IN denotes as
T2478 805-808 DT denotes the
T2479 809-817 JJ denotes standard
T2480 818-819 -LRB- denotes (
T2481 819-825 NNP denotes Pierce
T2482 826-838 NNP denotes Biochemicals
T2483 838-839 -COMMA- denotes ,
T2484 840-848 NNP denotes Rockford
T2485 848-849 -COMMA- denotes ,
T2486 850-852 NNP denotes IL
T2487 852-853 -COMMA- denotes ,
T2488 854-857 NNP denotes USA
T2489 857-858 -RRB- denotes )
T2490 860-864 NN denotes EMSA
T2491 865-868 VB denotes was
T2492 869-878 VB denotes performed
T2493 879-884 VB denotes using
T2494 885-888 NN denotes gel
T2495 889-894 NN denotes shift
T2496 895-900 NN denotes assay
T2497 901-907 NN denotes system
T2498 908-909 -LRB- denotes (
T2499 909-916 NNP denotes Promega
T2500 916-917 -COMMA- denotes ,
T2501 918-925 NNP denotes Madison
T2502 925-926 -COMMA- denotes ,
T2503 927-929 NNP denotes WI
T2504 929-930 -COMMA- denotes ,
T2505 931-934 NNP denotes USA
T2506 934-935 -RRB- denotes )
T2507 937-946 NNP denotes Consensus
T2508 947-962 NN denotes oligonucleotide
T2509 963-968 NN denotes probe
T2510 969-970 -LRB- denotes (
T2511 970-997 NN denotes 5′-AGTTGAGGGGACTTTCCCAGGG-3
T2512 997-998 -DQE- denotes ′
T2513 998-999 -RRB- denotes )
T2514 1000-1003 VB denotes was
T2515 1004-1015 VB denotes end-labeled
T2516 1016-1018 IN denotes by
T2517 1019-1036 NN denotes T4-polynucleotide
T2518 1037-1043 NN denotes kinase
T2519 1044-1049 VB denotes using
T2520 1050-1051 -LRB- denotes [
T2521 1051-1056 NN denotes γ-32P
T2522 1056-1057 -RRB- denotes ]
T2523 1057-1061 NN denotes -ATP
T2524 1062-1063 -LRB- denotes (
T2525 1063-1067 NNP denotes Free
T2526 1068-1076 NNP denotes Biotech.
T2527 1076-1077 -COMMA- denotes ,
T2528 1078-1085 NNP denotes Beijing
T2529 1085-1086 -COMMA- denotes ,
T2530 1087-1092 NNP denotes China
T2531 1092-1093 -RRB- denotes )
T2532 1095-1102 JJ denotes Nuclear
T2533 1103-1110 NN denotes protein
T2534 1111-1112 -LRB- denotes (
T2535 1112-1114 CD denotes 20
T2536 1115-1117 NN denotes μg
T2537 1117-1118 -RRB- denotes )
T2538 1119-1122 VB denotes was
T2539 1123-1135 VB denotes preincubated
T2540 1136-1138 IN denotes in
T2541 1139-1141 CD denotes 20
T2542 1142-1144 NN denotes μL
T2543 1145-1152 NN denotes binding
T2544 1153-1159 NN denotes buffer
T2545 1160-1170 VB denotes containing
T2546 1171-1173 CD denotes 10
T2547 1174-1176 NN denotes mM
T2548 1177-1185 NN denotes Tris-HCl
T2549 1186-1187 -LRB- denotes (
T2550 1187-1189 NN denotes PH
T2551 1190-1193 CD denotes 7.5
T2552 1193-1194 -RRB- denotes )
T2553 1194-1195 -COMMA- denotes ,
T2554 1196-1197 CD denotes 1
T2555 1198-1200 NN denotes mM
T2556 1201-1206 NN denotes MgCl2
T2557 1206-1207 -COMMA- denotes ,
T2558 1208-1211 CD denotes 0.5
T2559 1212-1214 NN denotes mM
T2560 1215-1219 NN denotes NaCl
T2561 1219-1220 -COMMA- denotes ,
T2562 1221-1222 CD denotes 4
T2563 1222-1223 NN denotes %
T2564 1224-1232 NN denotes glycerol
T2565 1232-1233 -COMMA- denotes ,
T2566 1234-1237 CD denotes 0.5
T2567 1238-1240 NN denotes mM
T2568 1241-1245 NN denotes EDTA
T2569 1245-1246 -COMMA- denotes ,
T2570 1247-1250 CD denotes 0.5
T2571 1251-1253 NN denotes mM
T2572 1254-1257 NN denotes DTT
T2573 1257-1258 -COMMA- denotes ,
T2574 1259-1262 CC denotes and
T2575 1263-1264 CD denotes 2
T2576 1265-1267 NN denotes μg
T2577 1268-1272 NN denotes poly
T2578 1273-1278 NN denotes dI-dC
T2579 1279-1282 IN denotes for
T2580 1283-1285 CD denotes 20
T2581 1286-1293 NN denotes minutes
T2582 1294-1296 IN denotes on
T2583 1297-1300 NN denotes ice
T2584 1302-1307 IN denotes After
T2585 1308-1316 NN denotes addition
T2586 1317-1319 IN denotes of
T2587 1320-1323 DT denotes the
T2588 1324-1325 CD denotes 1
T2589 1326-1328 NN denotes μL
T2590 1329-1339 JJ denotes 32P-labled
T2591 1340-1355 NN denotes oligonucleotide
T2592 1356-1361 NN denotes probe
T2593 1361-1362 -COMMA- denotes ,
T2594 1363-1366 DT denotes the
T2595 1367-1377 NN denotes incubation
T2596 1378-1381 VB denotes was
T2597 1382-1391 VB denotes continued
T2598 1392-1395 IN denotes for
T2599 1396-1398 CD denotes 20
T2600 1399-1406 NN denotes minutes
T2601 1407-1409 IN denotes on
T2602 1410-1413 NN denotes ice
T2603 1415-1418 DT denotes The
T2604 1419-1430 JJ denotes DNA-protein
T2605 1431-1440 NN denotes complexes
T2606 1441-1445 VB denotes were
T2607 1446-1455 VB denotes separated
T2608 1456-1458 IN denotes by
T2609 1459-1474 NN denotes electrophoresis
T2610 1475-1477 IN denotes on
T2611 1478-1479 CD denotes 4
T2612 1479-1480 NN denotes %
T2613 1481-1494 JJ denotes nondenaturing
T2614 1495-1509 NN denotes polyacrylamide
T2615 1510-1513 NN denotes gel
T2616 1514-1516 IN denotes in
T2617 1517-1520 CD denotes 0.5
T2618 1521-1522 SYM denotes ×
T2619 1523-1526 NN denotes TBE
T2620 1527-1533 NN denotes buffer
T2621 1534-1535 -LRB- denotes (
T2622 1535-1551 NN denotes tris-borate-EDTA
T2623 1551-1552 -RRB- denotes )
T2624 1553-1555 IN denotes at
T2625 1556-1559 CD denotes 390
T2626 1560-1561 NN denotes V
T2627 1562-1565 IN denotes for
T2628 1566-1567 CD denotes 1
T2629 1568-1572 NN denotes hour
T2630 1573-1575 IN denotes at
T2631 1576-1579 NN denotes 4°C
T2632 1581-1586 IN denotes After
T2633 1587-1602 NN denotes electrophoresis
T2634 1602-1603 -COMMA- denotes ,
T2635 1604-1607 DT denotes the
T2636 1608-1611 NN denotes gel
T2637 1612-1615 VB denotes was
T2638 1616-1621 VB denotes dried
T2639 1622-1625 CC denotes and
T2640 1626-1633 VB denotes exposed
T2641 1634-1636 TO denotes to
T2642 1637-1642 NN denotes X-ray
T2643 1643-1647 NN denotes film
T2644 1648-1649 -LRB- denotes (
T2645 1649-1653 NNP denotes Fuji
T2646 1654-1663 NNP denotes Hyperfilm
T2647 1663-1664 -COMMA- denotes ,
T2648 1665-1670 NNP denotes Tokyo
T2649 1670-1671 -COMMA- denotes ,
T2650 1672-1677 NNP denotes Japan
T2651 1677-1678 -RRB- denotes )
T2652 1680-1686 NN denotes Levels
T2653 1687-1689 IN denotes of
T2654 1690-1695 NN denotes NF-κB
T2655 1696-1699 NN denotes DNA
T2656 1700-1707 NN denotes binding
T2657 1708-1716 NN denotes activity
T2658 1717-1721 VB denotes were
T2659 1722-1732 VB denotes quantified
T2660 1733-1735 IN denotes by
T2661 1736-1744 NN denotes software
T2662 1745-1751 NN denotes ImageJ
R1974 T2331 T2332 arg1Of Monolayers,of
R1975 T2331 T2334 arg1Of Monolayers,were
R1976 T2331 T2335 arg2Of Monolayers,washed
R1977 T2331 T2339 arg2Of Monolayers,harvested
R1978 T2333 T2332 arg2Of astrocytes,of
R1979 T2335 T2336 arg1Of washed,with
R1980 T2335 T2338 arg1Of washed,and
R1981 T2337 T2336 arg2Of PBS,with
R1982 T2338 T2334 arg2Of and,were
R1983 T2339 T2338 arg2Of harvested,and
R1984 T2339 T2340 arg1Of harvested,by
R1985 T2341 T2340 arg2Of scraping,by
R1986 T2341 T2342 arg1Of scraping,into
R1987 T2344 T2342 arg2Of PBS,into
R1988 T2344 T2343 arg1Of PBS,cold
R1989 T2347 T2345 arg1Of pellet,The
R1990 T2347 T2346 arg1Of pellet,cell
R1991 T2347 T2348 arg2Of pellet,obtained
R1992 T2347 T2351 arg1Of pellet,was
R1993 T2347 T2352 arg2Of pellet,resuspended
R1994 T2350 T2348 arg1Of centrifugation,obtained
R1995 T2350 T2349 arg2Of centrifugation,by
R1996 T2352 T2351 arg2Of resuspended,was
R1997 T2352 T2353 arg1Of resuspended,in
R1998 T2354 T2355 arg1Of buffer,containing
R1999 T2354 T2367 arg1Of buffer,","
R2000 T2356 T2357 arg1Of 10,mM
R2001 T2358 T2356 arg1Of HEPES,10
R2002 T2358 T2359 arg1Of HEPES,(
R2003 T2358 T2363 arg1Of HEPES,","
R2004 T2360 T2359 arg2Of pH,(
R2005 T2360 T2361 arg1Of pH,7.9
R2006 T2362 T2359 arg3Of ),(
R2007 T2363 T2355 arg2Of ",",containing
R2008 T2364 T2365 arg1Of 10,mM
R2009 T2366 T2363 arg2Of KCl,","
R2010 T2366 T2364 arg1Of KCl,10
R2011 T2367 T2353 arg2Of ",",in
R2012 T2368 T2369 arg1Of 0.1,mM
R2013 T2370 T2368 arg1Of EDTA,0.1
R2014 T2370 T2371 arg1Of EDTA,","
R2015 T2371 T2375 arg1Of ",",","
R2016 T2372 T2373 arg1Of 0.1,mM
R2017 T2374 T2371 arg2Of EGTA,","
R2018 T2374 T2372 arg1Of EGTA,0.1
R2019 T2375 T2380 arg1Of ",",and
R2020 T2376 T2377 arg1Of 1.0,mM
R2021 T2378 T2375 arg2Of DTT,","
R2022 T2378 T2376 arg1Of DTT,1.0
R2023 T2380 T2367 arg2Of and,","
R2024 T2380 T2379 arg1Of and,","
R2025 T2381 T2382 arg1Of 0.5,mM
R2026 T2384 T2380 arg2Of fluoride,and
R2027 T2384 T2381 arg1Of fluoride,0.5
R2028 T2384 T2383 arg1Of fluoride,phenylmethylsulfonyl
R2029 T2386 T2385 arg1Of 10,Then
R2030 T2386 T2387 arg1Of 10,%
R2031 T2389 T2386 arg1Of P-40,10
R2032 T2389 T2388 arg1Of P-40,Nonidet
R2033 T2389 T2390 arg1Of P-40,was
R2034 T2389 T2391 arg2Of P-40,added
R2035 T2389 T2393 arg2Of P-40,vortexed
R2036 T2391 T2392 arg1Of added,and
R2037 T2392 T2390 arg2Of and,was
R2038 T2392 T2396 arg1Of and,and
R2039 T2393 T2392 arg2Of vortexed,and
R2040 T2394 T2393 arg3Of briefly,vortexed
R2041 T2396 T2395 arg1Of and,","
R2042 T2398 T2397 arg1Of nuclei,the
R2043 T2398 T2399 arg1Of nuclei,were
R2044 T2398 T2400 arg2Of nuclei,pelleted
R2045 T2400 T2396 arg2Of pelleted,and
R2046 T2400 T2399 arg2Of pelleted,were
R2047 T2402 T2400 arg1Of centrifugation,pelleted
R2048 T2402 T2401 arg2Of centrifugation,by
R2049 T2405 T2403 arg1Of proteins,The
R2050 T2405 T2404 arg1Of proteins,nuclear
R2051 T2405 T2406 arg1Of proteins,were
R2052 T2405 T2407 arg2Of proteins,extracted
R2053 T2407 T2406 arg2Of extracted,were
R2054 T2407 T2408 arg1Of extracted,with
R2055 T2409 T2410 arg1Of buffer,containing
R2056 T2409 T2422 arg1Of buffer,","
R2057 T2411 T2412 arg1Of 20,mM
R2058 T2413 T2411 arg1Of HEPES,20
R2059 T2413 T2414 arg1Of HEPES,(
R2060 T2413 T2418 arg1Of HEPES,","
R2061 T2415 T2414 arg2Of pH,(
R2062 T2415 T2416 arg1Of pH,7.9
R2063 T2417 T2414 arg3Of ),(
R2064 T2418 T2410 arg2Of ",",containing
R2065 T2419 T2420 arg1Of 0.4,mM
R2066 T2421 T2418 arg2Of NaCl,","
R2067 T2421 T2419 arg1Of NaCl,0.4
R2068 T2422 T2408 arg2Of ",",with
R2069 T2423 T2424 arg1Of 1.0,mM
R2070 T2425 T2423 arg1Of EDTA,1.0
R2071 T2425 T2426 arg1Of EDTA,","
R2072 T2426 T2430 arg1Of ",",","
R2073 T2427 T2428 arg1Of 1.0,mM
R2074 T2429 T2426 arg2Of EGTA,","
R2075 T2429 T2427 arg1Of EGTA,1.0
R2076 T2430 T2435 arg1Of ",",and
R2077 T2431 T2432 arg1Of 1.0,mM
R2078 T2433 T2430 arg2Of DTT,","
R2079 T2433 T2431 arg1Of DTT,1.0
R2080 T2435 T2422 arg2Of and,","
R2081 T2435 T2434 arg1Of and,","
R2082 T2436 T2437 arg1Of 1.0,mM
R2083 T2439 T2435 arg2Of fluoride,and
R2084 T2439 T2436 arg1Of fluoride,1.0
R2085 T2439 T2438 arg1Of fluoride,phenylmethylsulfonyl
R2086 T2441 T2440 arg1Of material,Insoluble
R2087 T2441 T2442 arg1Of material,was
R2088 T2441 T2443 arg2Of material,removed
R2089 T2443 T2442 arg2Of removed,was
R2090 T2443 T2446 arg1Of removed,at
R2091 T2443 T2450 arg1Of removed,and
R2092 T2445 T2443 arg1Of centrifugation,removed
R2093 T2445 T2444 arg2Of centrifugation,by
R2094 T2448 T2446 arg2Of rpm,at
R2095 T2448 T2447 arg1Of rpm,14000
R2096 T2450 T2449 arg1Of and,","
R2097 T2452 T2451 arg1Of supernatant,the
R2098 T2452 T2453 arg1Of supernatant,containing
R2099 T2452 T2457 arg1Of supernatant,was
R2100 T2452 T2458 arg2Of supernatant,stored
R2101 T2456 T2453 arg2Of proteins,containing
R2102 T2456 T2454 arg1Of proteins,the
R2103 T2456 T2455 arg1Of proteins,nuclear
R2104 T2458 T2450 arg2Of stored,and
R2105 T2458 T2457 arg2Of stored,was
R2106 T2458 T2459 arg1Of stored,at
R2107 T2458 T2461 arg1Of stored,until
R2108 T2460 T2459 arg2Of –80°C,at
R2109 T2462 T2461 arg2Of use,until
R2110 T2464 T2463 arg1Of concentration,Protein
R2111 T2464 T2465 arg1Of concentration,was
R2112 T2464 T2466 arg2Of concentration,determined
R2113 T2466 T2465 arg2Of determined,was
R2114 T2467 T2466 arg3Of using,determined
R2115 T2467 T2473 arg1Of using,with
R2116 T2472 T2467 arg2Of kit,using
R2117 T2472 T2468 arg1Of kit,a
R2118 T2472 T2469 arg1Of kit,bicinchoninic
R2119 T2472 T2470 arg1Of kit,acid
R2120 T2472 T2471 arg1Of kit,assay
R2121 T2476 T2473 arg2Of albumin,with
R2122 T2476 T2474 arg1Of albumin,bovine
R2123 T2476 T2475 arg1Of albumin,serum
R2124 T2476 T2477 arg1Of albumin,as
R2125 T2482 T2481 arg1Of Biochemicals,Pierce
R2126 T2482 T2483 arg1Of Biochemicals,","
R2127 T2483 T2485 arg1Of ",",","
R2128 T2484 T2483 arg2Of Rockford,","
R2129 T2485 T2477 arg2Of ",",as
R2130 T2485 T2478 arg1Of ",",the
R2131 T2485 T2479 arg1Of ",",standard
R2132 T2485 T2480 arg2Of ",",(
R2133 T2485 T2487 arg1Of ",",","
R2134 T2485 T2488 arg1Of ",",USA
R2135 T2486 T2485 arg2Of IL,","
R2136 T2489 T2480 arg3Of ),(
R2137 T2490 T2491 arg1Of EMSA,was
R2138 T2490 T2492 arg2Of EMSA,performed
R2139 T2492 T2491 arg2Of performed,was
R2140 T2493 T2492 arg3Of using,performed
R2141 T2497 T2493 arg2Of system,using
R2142 T2497 T2494 arg1Of system,gel
R2143 T2497 T2495 arg1Of system,shift
R2144 T2497 T2496 arg1Of system,assay
R2145 T2497 T2498 arg1Of system,(
R2146 T2499 T2500 arg1Of Promega,","
R2147 T2500 T2502 arg1Of ",",","
R2148 T2501 T2500 arg2Of Madison,","
R2149 T2502 T2498 arg2Of ",",(
R2150 T2502 T2504 arg1Of ",",","
R2151 T2502 T2505 arg1Of ",",USA
R2152 T2503 T2502 arg2Of WI,","
R2153 T2506 T2498 arg3Of ),(
R2154 T2509 T2507 arg1Of probe,Consensus
R2155 T2509 T2508 arg1Of probe,oligonucleotide
R2156 T2509 T2510 arg1Of probe,(
R2157 T2509 T2514 arg1Of probe,was
R2158 T2509 T2515 arg2Of probe,end-labeled
R2159 T2509 T2519 arg1Of probe,using
R2160 T2511 T2510 arg2Of 5′-AGTTGAGGGGACTTTCCCAGGG-3,(
R2161 T2511 T2512 arg1Of 5′-AGTTGAGGGGACTTTCCCAGGG-3,′
R2162 T2513 T2510 arg3Of ),(
R2163 T2515 T2514 arg2Of end-labeled,was
R2164 T2515 T2519 modOf end-labeled,using
R2165 T2518 T2515 arg1Of kinase,end-labeled
R2166 T2518 T2516 arg2Of kinase,by
R2167 T2518 T2517 arg1Of kinase,T4-polynucleotide
R2168 T2521 T2520 arg2Of γ-32P,[
R2169 T2522 T2520 arg3Of ],[
R2170 T2523 T2519 arg2Of -ATP,using
R2171 T2523 T2520 arg1Of -ATP,[
R2172 T2523 T2524 arg1Of -ATP,(
R2173 T2526 T2524 arg2Of Biotech.,(
R2174 T2526 T2525 arg1Of Biotech.,Free
R2175 T2526 T2527 arg1Of Biotech.,","
R2176 T2528 T2527 arg2Of Beijing,","
R2177 T2528 T2529 arg1Of Beijing,","
R2178 T2530 T2529 arg2Of China,","
R2179 T2531 T2524 arg3Of ),(
R2180 T2533 T2532 arg1Of protein,Nuclear
R2181 T2533 T2534 arg1Of protein,(
R2182 T2533 T2538 arg1Of protein,was
R2183 T2533 T2539 arg2Of protein,preincubated
R2184 T2536 T2534 arg2Of μg,(
R2185 T2536 T2535 arg1Of μg,20
R2186 T2537 T2534 arg3Of ),(
R2187 T2539 T2538 arg2Of preincubated,was
R2188 T2539 T2540 arg1Of preincubated,in
R2189 T2544 T2540 arg2Of buffer,in
R2190 T2544 T2541 arg1Of buffer,20
R2191 T2544 T2542 arg1Of buffer,μL
R2192 T2544 T2543 arg1Of buffer,binding
R2193 T2544 T2545 arg1Of buffer,containing
R2194 T2546 T2547 arg1Of 10,mM
R2195 T2548 T2546 arg1Of Tris-HCl,10
R2196 T2548 T2549 arg1Of Tris-HCl,(
R2197 T2548 T2553 arg1Of Tris-HCl,","
R2198 T2550 T2549 arg2Of PH,(
R2199 T2550 T2551 arg1Of PH,7.5
R2200 T2552 T2549 arg3Of ),(
R2201 T2553 T2557 arg1Of ",",","
R2202 T2554 T2555 arg1Of 1,mM
R2203 T2556 T2553 arg2Of MgCl2,","
R2204 T2556 T2554 arg1Of MgCl2,1
R2205 T2557 T2561 arg1Of ",",","
R2206 T2558 T2559 arg1Of 0.5,mM
R2207 T2560 T2557 arg2Of NaCl,","
R2208 T2560 T2558 arg1Of NaCl,0.5
R2209 T2561 T2545 arg2Of ",",containing
R2210 T2562 T2563 arg1Of 4,%
R2211 T2564 T2562 arg1Of glycerol,4
R2212 T2564 T2565 arg1Of glycerol,","
R2213 T2565 T2569 arg1Of ",",","
R2214 T2566 T2567 arg1Of 0.5,mM
R2215 T2568 T2565 arg2Of EDTA,","
R2216 T2568 T2566 arg1Of EDTA,0.5
R2217 T2569 T2574 arg1Of ",",and
R2218 T2570 T2571 arg1Of 0.5,mM
R2219 T2572 T2569 arg2Of DTT,","
R2220 T2572 T2570 arg1Of DTT,0.5
R2221 T2574 T2561 arg2Of and,","
R2222 T2574 T2573 arg1Of and,","
R2223 T2578 T2574 arg2Of dI-dC,and
R2224 T2578 T2575 arg1Of dI-dC,2
R2225 T2578 T2576 arg1Of dI-dC,μg
R2226 T2578 T2577 arg1Of dI-dC,poly
R2227 T2578 T2579 arg1Of dI-dC,for
R2228 T2581 T2579 arg2Of minutes,for
R2229 T2581 T2580 arg1Of minutes,20
R2230 T2581 T2582 arg1Of minutes,on
R2231 T2583 T2582 arg2Of ice,on
R2232 T2585 T2584 arg2Of addition,After
R2233 T2585 T2586 arg1Of addition,of
R2234 T2592 T2586 arg2Of probe,of
R2235 T2592 T2587 arg1Of probe,the
R2236 T2592 T2588 arg1Of probe,1
R2237 T2592 T2589 arg1Of probe,μL
R2238 T2592 T2590 arg1Of probe,32P-labled
R2239 T2592 T2591 arg1Of probe,oligonucleotide
R2240 T2595 T2594 arg1Of incubation,the
R2241 T2595 T2596 arg1Of incubation,was
R2242 T2595 T2597 arg2Of incubation,continued
R2243 T2597 T2584 arg1Of continued,After
R2244 T2597 T2593 arg1Of continued,","
R2245 T2597 T2596 arg2Of continued,was
R2246 T2597 T2598 arg1Of continued,for
R2247 T2600 T2598 arg2Of minutes,for
R2248 T2600 T2599 arg1Of minutes,20
R2249 T2600 T2601 arg1Of minutes,on
R2250 T2602 T2601 arg2Of ice,on
R2251 T2605 T2603 arg1Of complexes,The
R2252 T2605 T2604 arg1Of complexes,DNA-protein
R2253 T2605 T2606 arg1Of complexes,were
R2254 T2605 T2607 arg2Of complexes,separated
R2255 T2607 T2606 arg2Of separated,were
R2256 T2607 T2610 arg1Of separated,on
R2257 T2607 T2616 arg1Of separated,in
R2258 T2609 T2607 arg1Of electrophoresis,separated
R2259 T2609 T2608 arg2Of electrophoresis,by
R2260 T2611 T2612 arg1Of 4,%
R2261 T2615 T2610 arg2Of gel,on
R2262 T2615 T2611 arg1Of gel,4
R2263 T2615 T2613 arg1Of gel,nondenaturing
R2264 T2615 T2614 arg1Of gel,polyacrylamide
R2265 T2620 T2616 arg2Of buffer,in
R2266 T2620 T2617 arg1Of buffer,0.5
R2267 T2620 T2618 arg1Of buffer,×
R2268 T2620 T2619 arg1Of buffer,TBE
R2269 T2620 T2621 arg1Of buffer,(
R2270 T2620 T2624 arg1Of buffer,at
R2271 T2622 T2621 arg2Of tris-borate-EDTA,(
R2272 T2623 T2621 arg3Of ),(
R2273 T2626 T2624 arg2Of V,at
R2274 T2626 T2625 arg1Of V,390
R2275 T2626 T2627 arg1Of V,for
R2276 T2629 T2627 arg2Of hour,for
R2277 T2629 T2628 arg1Of hour,1
R2278 T2629 T2630 arg1Of hour,at
R2279 T2631 T2630 arg2Of 4°C,at
R2280 T2633 T2632 arg2Of electrophoresis,After
R2281 T2636 T2635 arg1Of gel,the
R2282 T2636 T2637 arg1Of gel,was
R2283 T2636 T2638 arg2Of gel,dried
R2284 T2636 T2640 arg2Of gel,exposed
R2285 T2638 T2639 arg1Of dried,and
R2286 T2639 T2632 arg1Of and,After
R2287 T2639 T2634 arg1Of and,","
R2288 T2639 T2637 arg2Of and,was
R2289 T2640 T2639 arg2Of exposed,and
R2290 T2640 T2641 arg1Of exposed,to
R2291 T2643 T2641 arg2Of film,to
R2292 T2643 T2642 arg1Of film,X-ray
R2293 T2643 T2644 arg1Of film,(
R2294 T2646 T2644 arg2Of Hyperfilm,(
R2295 T2646 T2645 arg1Of Hyperfilm,Fuji
R2296 T2646 T2647 arg1Of Hyperfilm,","
R2297 T2648 T2647 arg2Of Tokyo,","
R2298 T2648 T2649 arg1Of Tokyo,","
R2299 T2650 T2649 arg2Of Japan,","
R2300 T2651 T2644 arg3Of ),(
R2301 T2652 T2653 arg1Of Levels,of
R2302 T2652 T2658 arg1Of Levels,were
R2303 T2652 T2659 arg2Of Levels,quantified
R2304 T2657 T2653 arg2Of activity,of
R2305 T2657 T2654 arg1Of activity,NF-κB
R2306 T2657 T2655 arg1Of activity,DNA
R2307 T2657 T2656 arg1Of activity,binding
R2308 T2659 T2658 arg2Of quantified,were
R2309 T2662 T2659 arg1Of ImageJ,quantified
R2310 T2662 T2660 arg2Of ImageJ,by
R2311 T2662 T2661 arg1Of ImageJ,software