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PMC:3317373 / 2853-11658 JSONTXT 25 Projects

Annnotations TAB TSV DIC JSON TextAE

Id Subject Object Predicate Lexical cue
T1240 26-29 CD denotes 2.1
T1241 31-38 JJ denotes Primary
T1242 39-46 NN denotes Culture
T1243 47-50 CC denotes and
T1244 51-65 NN denotes Identification
T1245 66-68 IN denotes of
T1246 69-74 NN denotes Mouse
T1247 75-85 NN denotes Astrocytes
T1248 86-90 NN denotes Nrf2
T1249 91-99 NN denotes knockout
T1250 100-103 NN denotes ICR
T1251 104-108 NN denotes mice
T1252 109-113 VB denotes were
T1253 114-120 RB denotes kindly
T1254 121-129 VB denotes provided
T1255 130-132 IN denotes by
T1256 133-136 NNP denotes Dr.
T1257 137-143 NNP denotes Thomas
T1258 144-146 NNP denotes W.
T1259 147-154 NNP denotes Kensler
T1260 155-156 -LRB- denotes (
T1261 156-161 NNP denotes Johns
T1262 162-169 NNP denotes Hopkins
T1263 170-180 NNP denotes University
T1264 180-181 -COMMA- denotes ,
T1265 182-191 NNP denotes Baltimore
T1266 191-192 -COMMA- denotes ,
T1267 193-195 NNP denotes MD
T1268 195-196 -COMMA- denotes ,
T1269 197-200 NNP denotes USA
T1270 200-201 -RRB- denotes )
T1271 203-210 JJ denotes Primary
T1272 211-221 NN denotes astrocytes
T1273 222-226 VB denotes were
T1274 227-235 VB denotes obtained
T1275 236-240 IN denotes from
T1276 241-250 JJ denotes postnatal
T1277 251-260 JJ denotes 2-day-old
T1278 261-265 NN denotes Nrf2
T1279 266-270 JJ denotes wide
T1280 271-275 NN denotes type
T1281 276-277 -LRB- denotes (
T1282 277-279 NN denotes WT
T1283 279-280 -RRB- denotes )
T1284 281-284 CC denotes and
T1285 285-293 NN denotes knockout
T1286 294-295 -LRB- denotes (
T1287 295-297 NN denotes KO
T1288 297-298 -RRB- denotes )
T1289 299-303 NN denotes mice
T1290 304-305 -LRB- denotes (
T1291 305-306 CD denotes 6
T1292 307-311 NN denotes mice
T1293 312-315 IN denotes for
T1294 316-320 DT denotes each
T1295 321-329 NN denotes genotype
T1296 329-330 -RRB- denotes )
T1297 332-341 VB denotes Following
T1298 342-354 NN denotes decapitation
T1299 354-355 -COMMA- denotes ,
T1300 356-359 DT denotes the
T1301 360-368 NN denotes cortices
T1302 369-373 VB denotes were
T1303 374-383 VB denotes dissected
T1304 384-387 RP denotes out
T1305 387-388 -COMMA- denotes ,
T1306 389-392 CC denotes and
T1307 393-396 DT denotes the
T1308 397-405 NN denotes meninges
T1309 406-409 CC denotes and
T1310 410-420 VB denotes associated
T1311 421-426 NN denotes blood
T1312 427-434 NN denotes vessels
T1313 435-439 VB denotes were
T1314 440-447 VB denotes removed
T1315 449-452 DT denotes The
T1316 453-459 NN denotes tissue
T1317 460-463 VB denotes was
T1318 464-471 RB denotes roughly
T1319 472-479 VB denotes chopped
T1320 480-484 IN denotes with
T1321 485-486 DT denotes a
T1322 487-494 NN denotes scalpel
T1323 495-500 NN denotes blade
T1324 500-501 -COMMA- denotes ,
T1325 502-511 VB denotes incubated
T1326 512-514 IN denotes in
T1327 515-518 CD denotes 0.5
T1328 518-519 NN denotes %
T1329 520-527 NN denotes trypsin
T1330 528-531 IN denotes for
T1331 532-534 CD denotes 10
T1332 535-542 NN denotes minutes
T1333 543-545 IN denotes at
T1334 546-550 NN denotes 37°C
T1335 550-551 -COMMA- denotes ,
T1336 552-555 CC denotes and
T1337 556-564 VB denotes agitated
T1338 565-570 DT denotes every
T1339 571-574 JJ denotes few
T1340 575-582 NN denotes minutes
T1341 584-589 IN denotes After
T1342 590-599 NN denotes digestion
T1343 599-600 -COMMA- denotes ,
T1344 601-604 DT denotes the
T1345 605-611 NN denotes tissue
T1346 612-615 VB denotes was
T1347 616-622 VB denotes rinsed
T1348 623-628 RB denotes twice
T1349 629-631 IN denotes in
T1350 632-636 NN denotes DMEM
T1351 637-641 IN denotes with
T1352 642-644 CD denotes 10
T1353 644-645 NN denotes %
T1354 646-649 NNP denotes FBS
T1355 649-650 -COMMA- denotes ,
T1356 651-659 VB denotes followed
T1357 660-662 IN denotes by
T1358 663-664 DT denotes a
T1359 665-675 JJ denotes mechanical
T1360 676-688 NN denotes dissociation
T1361 689-691 IN denotes in
T1362 692-696 NN denotes DMEM
T1363 697-701 IN denotes with
T1364 702-704 CD denotes 20
T1365 704-705 NN denotes %
T1366 706-709 NN denotes FBS
T1367 710-713 CC denotes and
T1368 714-715 CD denotes 5
T1369 716-724 NN denotes units/mL
T1370 725-735 NN denotes penicillin
T1371 735-736 -COMMA- denotes ,
T1372 737-738 CD denotes 5
T1373 739-744 NN denotes μg/mL
T1374 745-757 NN denotes streptomycin
T1375 758-759 -LRB- denotes (
T1376 759-767 JJ denotes complete
T1377 768-775 NN denotes culture
T1378 776-782 NN denotes medium
T1379 782-783 -RRB- denotes )
T1380 785-790 IN denotes After
T1381 791-801 NN denotes incubation
T1382 802-805 IN denotes for
T1383 806-807 CD denotes 1
T1384 808-809 NN denotes h
T1385 809-810 -COMMA- denotes ,
T1386 811-814 DT denotes the
T1387 815-826 NN denotes supernatant
T1388 827-830 VB denotes was
T1389 831-842 VB denotes transferred
T1390 843-845 TO denotes to
T1391 846-847 DT denotes a
T1392 848-851 JJ denotes new
T1393 852-857 NN denotes flask
T1394 858-860 CC denotes or
T1395 861-865 JJ denotes dish
T1396 866-867 -LRB- denotes (
T1397 867-873 NNP denotes Costar
T1398 873-874 -COMMA- denotes ,
T1399 875-878 NNP denotes USA
T1400 878-879 -RRB- denotes )
T1401 880-883 IN denotes for
T1402 884-893 VB denotes depleting
T1403 894-897 DT denotes the
T1404 898-906 JJ denotes residual
T1405 907-917 JJ denotes epithelial
T1406 918-923 NN denotes cells
T1407 925-929 RB denotes Then
T1408 930-933 DT denotes the
T1409 934-939 NN denotes cells
T1410 940-944 VB denotes were
T1411 945-955 VB denotes cultivated
T1412 956-958 IN denotes at
T1413 959-963 NN denotes 37°C
T1414 964-968 IN denotes with
T1415 969-970 CD denotes 5
T1416 970-971 NN denotes %
T1417 972-975 NN denotes CO2
T1418 977-980 CC denotes And
T1419 981-984 DT denotes the
T1420 985-993 JJ denotes complete
T1421 994-1001 NN denotes culture
T1422 1002-1008 NN denotes medium
T1423 1009-1012 VB denotes was
T1424 1013-1025 JJ denotes half-changed
T1425 1026-1031 RB denotes twice
T1426 1032-1033 DT denotes a
T1427 1034-1038 NN denotes week
T1428 1040-1050 NN denotes Astrocytes
T1429 1051-1059 VB denotes expanded
T1430 1060-1063 IN denotes for
T1431 1064-1069 IN denotes about
T1432 1070-1071 CD denotes 7
T1433 1072-1076 NN denotes days
T1434 1077-1079 TO denotes to
T1435 1080-1085 VB denotes reach
T1436 1086-1096 NN denotes confluence
T1437 1098-1102 RB denotes Then
T1438 1103-1106 DT denotes the
T1439 1107-1113 NN denotes flasks
T1440 1114-1116 CC denotes or
T1441 1117-1123 NN denotes dishes
T1442 1124-1128 VB denotes were
T1443 1129-1135 VB denotes shaken
T1444 1136-1138 IN denotes at
T1445 1139-1142 CD denotes 150
T1446 1143-1146 NN denotes rpm
T1447 1147-1150 IN denotes for
T1448 1151-1152 CD denotes 4
T1449 1153-1154 NN denotes h
T1450 1155-1157 TO denotes to
T1451 1158-1165 VB denotes deplete
T1452 1166-1169 DT denotes the
T1453 1170-1179 NN denotes microglia
T1454 1180-1183 CC denotes and
T1455 1184-1188 RB denotes less
T1456 1189-1197 JJ denotes adherent
T1457 1198-1203 NN denotes cells
T1458 1204-1208 IN denotes from
T1459 1209-1212 DT denotes the
T1460 1213-1221 NN denotes cultures
T1461 1223-1228 IN denotes After
T1462 1229-1236 VB denotes shaking
T1463 1236-1237 -COMMA- denotes ,
T1464 1238-1241 DT denotes the
T1465 1242-1251 VB denotes resulting
T1466 1252-1260 NN denotes cultures
T1467 1261-1265 VB denotes were
T1468 1266-1272 RB denotes mainly
T1469 1273-1283 NN denotes astrocytes
T1470 1283-1284 -COMMA- denotes ,
T1471 1285-1290 WDT denotes which
T1472 1291-1295 VB denotes were
T1473 1296-1306 VB denotes determined
T1474 1307-1309 IN denotes by
T1475 1310-1326 NN denotes immunoreactivity
T1476 1327-1330 IN denotes for
T1477 1331-1335 NN denotes GFAP
T1478 1336-1337 -LRB- denotes (
T1479 1337-1346 NN denotes sc-166481
T1480 1346-1347 -COMMA- denotes ,
T1481 1348-1353 NNP denotes Santa
T1482 1354-1358 NNP denotes Cruz
T1483 1359-1372 NNP denotes Biotechnology
T1484 1372-1373 -COMMA- denotes ,
T1485 1374-1376 NNP denotes CA
T1486 1376-1377 -COMMA- denotes ,
T1487 1378-1381 NNP denotes USA
T1488 1381-1382 -RRB- denotes )
T1489 1384-1389 NN denotes Cells
T1490 1390-1398 VB denotes passaged
T1491 1399-1402 IN denotes for
T1492 1403-1406 CD denotes 2-3
T1493 1407-1418 NN denotes generations
T1494 1419-1423 VB denotes were
T1495 1424-1428 VB denotes used
T1496 1429-1431 IN denotes in
T1497 1432-1435 DT denotes the
T1498 1436-1445 VB denotes following
T1499 1446-1453 NN denotes studies
T1821 1456-1459 CD denotes 2.2
T1822 1461-1463 IN denotes In
T1823 1464-1469 NNP denotes Vitro
T1824 1470-1475 NNP denotes Model
T1825 1476-1478 IN denotes of
T1826 1479-1482 NNP denotes TBI
T1827 1483-1494 VB denotes Established
T1828 1495-1497 IN denotes by
T1829 1498-1505 NNP denotes Scratch
T1830 1506-1512 NNP denotes Injury
T1831 1513-1522 NN denotes Astrocyte
T1832 1523-1530 NN denotes scratch
T1833 1531-1537 NN denotes injury
T1834 1538-1541 VB denotes was
T1835 1542-1551 VB denotes performed
T1836 1552-1554 IN denotes as
T1837 1555-1557 IN denotes in
T1838 1558-1559 DT denotes a
T1839 1560-1568 JJ denotes previous
T1840 1569-1575 NN denotes report
T1841 1576-1577 -LRB- denotes [
T1842 1577-1578 CD denotes 7
T1843 1578-1579 -RRB- denotes ]
T1844 1581-1591 NN denotes Astrocytes
T1845 1592-1596 VB denotes were
T1846 1597-1604 VB denotes planted
T1847 1605-1607 IN denotes in
T1848 1608-1614 JJ denotes 6-well
T1849 1615-1621 NN denotes plates
T1850 1622-1625 CC denotes and
T1851 1626-1631 VB denotes grown
T1852 1632-1634 TO denotes to
T1853 1635-1645 VB denotes confluence
T1854 1647-1650 DT denotes The
T1855 1651-1655 NN denotes cell
T1856 1656-1665 NN denotes monolayer
T1857 1666-1669 VB denotes was
T1858 1670-1679 VB denotes scratched
T1859 1680-1684 IN denotes with
T1860 1685-1686 DT denotes a
T1861 1687-1694 JJ denotes sterile
T1862 1695-1698 NN denotes 26G
T1863 1699-1706 NN denotes syringe
T1864 1707-1713 NN denotes needle
T1865 1713-1714 -COMMA- denotes ,
T1866 1715-1724 VB denotes resulting
T1867 1725-1727 IN denotes in
T1868 1728-1731 DT denotes the
T1869 1732-1741 NN denotes formation
T1870 1742-1744 IN denotes of
T1871 1745-1746 DT denotes a
T1872 1747-1750 CD denotes 0.5
T1873 1751-1753 NN denotes mm
T1874 1754-1758 JJ denotes wide
T1875 1759-1762 NN denotes gap
T1876 1764-1775 RB denotes Immediately
T1877 1776-1781 IN denotes after
T1878 1782-1789 NN denotes scratch
T1879 1789-1790 -COMMA- denotes ,
T1880 1791-1796 NN denotes cells
T1881 1797-1801 VB denotes were
T1882 1802-1808 VB denotes washed
T1883 1809-1814 RB denotes twice
T1884 1815-1819 IN denotes with
T1885 1820-1827 JJ denotes sterile
T1886 1828-1831 NNP denotes PBS
T1887 1831-1832 -COMMA- denotes ,
T1888 1833-1841 VB denotes cultured
T1889 1842-1846 IN denotes with
T1890 1847-1855 JJ denotes complete
T1891 1856-1863 NN denotes culture
T1892 1864-1870 NN denotes medium
T1893 1870-1871 -COMMA- denotes ,
T1894 1872-1875 CC denotes and
T1895 1876-1881 VB denotes named
T1896 1882-1884 IN denotes as
T1897 1885-1888 NN denotes TBI
T1898 1889-1894 NN denotes group
T1899 1896-1901 NN denotes Cells
T1900 1901-1902 -COMMA- denotes ,
T1901 1903-1908 WDT denotes which
T1902 1909-1912 VB denotes did
T1903 1913-1916 RB denotes not
T1904 1917-1925 VB denotes received
T1905 1926-1933 NN denotes scratch
T1906 1933-1934 -COMMA- denotes ,
T1907 1935-1939 VB denotes were
T1908 1940-1944 VB denotes used
T1909 1945-1947 IN denotes as
T1910 1948-1953 JJ denotes blank
T1911 1954-1961 NN denotes control
T1912 1962-1965 CC denotes and
T1913 1966-1971 VB denotes named
T1914 1972-1974 IN denotes as
T1915 1975-1979 NN denotes sham
T1916 1980-1985 NN denotes group
T2047 1988-1991 CD denotes 2.3
T2048 1993-1997 NNP denotes Cell
T2049 1998-2003 NNP denotes Death
T2050 2004-2015 NNP denotes Measurement
T2051 2016-2018 IN denotes by
T2052 2019-2024 NN denotes CCK-8
T2053 2025-2030 NN denotes Assay
T2054 2031-2032 CD denotes 1
T2055 2033-2034 SYM denotes ×
T2056 2035-2043 NN denotes 103/well
T2057 2044-2049 NN denotes cells
T2058 2050-2054 VB denotes were
T2059 2055-2061 VB denotes seeded
T2060 2062-2064 TO denotes to
T2061 2065-2072 JJ denotes 96-well
T2062 2073-2080 NN denotes culture
T2063 2081-2087 NN denotes plates
T2064 2088-2091 CC denotes and
T2065 2092-2102 VB denotes cultivated
T2066 2103-2106 IN denotes for
T2067 2107-2109 CD denotes 24
T2068 2110-2111 NN denotes h
T2069 2112-2114 TO denotes to
T2070 2115-2121 VB denotes adhere
T2071 2123-2127 RB denotes Then
T2072 2128-2131 DT denotes the
T2073 2132-2136 NN denotes cell
T2074 2137-2140 VB denotes was
T2075 2141-2148 VB denotes scrated
T2076 2149-2151 IN denotes as
T2077 2152-2161 VB denotes mentioned
T2078 2162-2167 IN denotes above
T2079 2169-2178 NN denotes Astrocyte
T2080 2179-2183 NN denotes cell
T2081 2184-2189 NN denotes death
T2082 2190-2193 VB denotes was
T2083 2194-2202 VB denotes assessed
T2084 2203-2205 IN denotes by
T2085 2206-2210 NN denotes cell
T2086 2211-2219 NN denotes counting
T2087 2220-2225 NN denotes kit-8
T2088 2226-2227 -LRB- denotes (
T2089 2227-2232 NN denotes CCK-8
T2090 2232-2233 -RRB- denotes )
T2091 2234-2235 -LRB- denotes (
T2092 2235-2242 NNP denotes Dojindo
T2093 2242-2243 -COMMA- denotes ,
T2094 2244-2249 NNP denotes Japan
T2095 2249-2250 -RRB- denotes )
T2096 2251-2256 NN denotes assay
T2097 2257-2259 CD denotes 24
T2098 2260-2261 NN denotes h
T2099 2262-2267 IN denotes after
T2100 2268-2275 NN denotes scratch
T2101 2276-2285 VB denotes according
T2102 2286-2288 TO denotes to
T2103 2289-2292 DT denotes the
T2104 2293-2305 NN denotes manufacturer
T2105 2305-2307 POS denotes 's
T2106 2308-2316 NN denotes protocol
T2107 2318-2325 NNP denotes Briefly
T2108 2325-2326 -COMMA- denotes ,
T2109 2327-2329 CD denotes 10
T2110 2330-2332 NN denotes μL
T2111 2333-2338 NN denotes CCK-8
T2112 2339-2342 VB denotes was
T2113 2343-2348 VB denotes added
T2114 2349-2353 IN denotes into
T2115 2354-2359 DT denotes every
T2116 2360-2364 RB denotes well
T2117 2365-2368 CC denotes and
T2118 2369-2378 VB denotes incubated
T2119 2379-2382 IN denotes for
T2120 2383-2384 CD denotes 1
T2121 2385-2386 NN denotes h
T2122 2388-2392 RB denotes Then
T2123 2393-2395 NN denotes OD
T2124 2396-2401 NN denotes value
T2125 2402-2405 VB denotes was
T2126 2406-2410 VB denotes read
T2127 2411-2413 IN denotes at
T2128 2414-2417 CD denotes 450
T2129 2418-2420 NN denotes nm
T2130 2421-2426 VB denotes using
T2131 2427-2428 DT denotes a
T2132 2429-2436 JJ denotes Bio-Rad
T2133 2437-2442 NN denotes ELISA
T2134 2443-2453 NN denotes microplate
T2135 2454-2460 NN denotes reader
T2136 2461-2462 -LRB- denotes (
T2137 2462-2469 NNP denotes Bio-Rad
T2138 2470-2482 NNP denotes Laboratories
T2139 2482-2483 -COMMA- denotes ,
T2140 2484-2486 NNP denotes CA
T2141 2486-2487 -COMMA- denotes ,
T2142 2488-2491 NNP denotes USA
T2143 2491-2492 -RRB- denotes )
T2144 2494-2497 DT denotes All
T2145 2498-2510 NN denotes measurements
T2146 2511-2515 VB denotes were
T2147 2516-2525 VB denotes performed
T2148 2526-2528 IN denotes in
T2149 2529-2541 NN denotes sextuplicate
T2150 2543-2550 NN denotes Results
T2151 2551-2555 VB denotes were
T2152 2556-2565 VB denotes expressed
T2153 2566-2568 IN denotes as
T2154 2569-2573 NN denotes mean
T2155 2574-2576 IN denotes of
T2156 2577-2579 NN denotes OD
T2157 2580-2585 NN denotes value
T2158 2586-2588 IN denotes at
T2159 2589-2592 CD denotes 450
T2160 2593-2595 NN denotes nm
T2161 2596-2597 SYM denotes ±
T2162 2598-2600 NN denotes SD
T2323 2603-2606 CD denotes 2.4
T2324 2608-2623 JJ denotes Electrophoretic
T2325 2624-2632 NN denotes Mobility
T2326 2633-2638 NN denotes Shift
T2327 2639-2644 NN denotes Assay
T2328 2645-2646 -LRB- denotes (
T2329 2646-2650 NN denotes EMSA
T2330 2650-2651 -RRB- denotes )
T2331 2652-2662 NN denotes Monolayers
T2332 2663-2665 IN denotes of
T2333 2666-2676 NN denotes astrocytes
T2334 2677-2681 VB denotes were
T2335 2682-2688 VB denotes washed
T2336 2689-2693 IN denotes with
T2337 2694-2697 NNP denotes PBS
T2338 2698-2701 CC denotes and
T2339 2702-2711 VB denotes harvested
T2340 2712-2714 IN denotes by
T2341 2715-2723 VB denotes scraping
T2342 2724-2728 IN denotes into
T2343 2729-2733 JJ denotes cold
T2344 2734-2737 NNP denotes PBS
T2345 2739-2742 DT denotes The
T2346 2743-2747 NN denotes cell
T2347 2748-2754 NN denotes pellet
T2348 2755-2763 VB denotes obtained
T2349 2764-2766 IN denotes by
T2350 2767-2781 NN denotes centrifugation
T2351 2782-2785 VB denotes was
T2352 2786-2797 VB denotes resuspended
T2353 2798-2800 IN denotes in
T2354 2801-2807 NN denotes buffer
T2355 2808-2818 VB denotes containing
T2356 2819-2821 CD denotes 10
T2357 2822-2824 NN denotes mM
T2358 2825-2830 NN denotes HEPES
T2359 2831-2832 -LRB- denotes (
T2360 2832-2834 NN denotes pH
T2361 2835-2838 CD denotes 7.9
T2362 2838-2839 -RRB- denotes )
T2363 2839-2840 -COMMA- denotes ,
T2364 2841-2843 CD denotes 10
T2365 2844-2846 NN denotes mM
T2366 2847-2850 NN denotes KCl
T2367 2850-2851 -COMMA- denotes ,
T2368 2852-2855 CD denotes 0.1
T2369 2856-2858 NN denotes mM
T2370 2859-2863 NN denotes EDTA
T2371 2863-2864 -COMMA- denotes ,
T2372 2865-2868 CD denotes 0.1
T2373 2869-2871 NN denotes mM
T2374 2872-2876 NN denotes EGTA
T2375 2876-2877 -COMMA- denotes ,
T2376 2878-2881 CD denotes 1.0
T2377 2882-2884 NN denotes mM
T2378 2885-2888 NN denotes DTT
T2379 2888-2889 -COMMA- denotes ,
T2380 2890-2893 CC denotes and
T2381 2894-2897 CD denotes 0.5
T2382 2898-2900 NN denotes mM
T2383 2901-2921 NN denotes phenylmethylsulfonyl
T2384 2922-2930 NN denotes fluoride
T2385 2932-2936 RB denotes Then
T2386 2937-2939 CD denotes 10
T2387 2939-2940 NN denotes %
T2388 2941-2948 NN denotes Nonidet
T2389 2949-2953 NN denotes P-40
T2390 2954-2957 VB denotes was
T2391 2958-2963 VB denotes added
T2392 2964-2967 CC denotes and
T2393 2968-2976 VB denotes vortexed
T2394 2977-2984 NN denotes briefly
T2395 2984-2985 -COMMA- denotes ,
T2396 2986-2989 CC denotes and
T2397 2990-2993 DT denotes the
T2398 2994-3000 NN denotes nuclei
T2399 3001-3005 VB denotes were
T2400 3006-3014 VB denotes pelleted
T2401 3015-3017 IN denotes by
T2402 3018-3032 NN denotes centrifugation
T2403 3034-3037 DT denotes The
T2404 3038-3045 JJ denotes nuclear
T2405 3046-3054 NN denotes proteins
T2406 3055-3059 VB denotes were
T2407 3060-3069 VB denotes extracted
T2408 3070-3074 IN denotes with
T2409 3075-3081 NN denotes buffer
T2410 3082-3092 VB denotes containing
T2411 3093-3095 CD denotes 20
T2412 3096-3098 NN denotes mM
T2413 3099-3104 NN denotes HEPES
T2414 3105-3106 -LRB- denotes (
T2415 3106-3108 NN denotes pH
T2416 3109-3112 CD denotes 7.9
T2417 3112-3113 -RRB- denotes )
T2418 3113-3114 -COMMA- denotes ,
T2419 3115-3118 CD denotes 0.4
T2420 3119-3121 NN denotes mM
T2421 3122-3126 NN denotes NaCl
T2422 3126-3127 -COMMA- denotes ,
T2423 3128-3131 CD denotes 1.0
T2424 3132-3134 NN denotes mM
T2425 3135-3139 NN denotes EDTA
T2426 3139-3140 -COMMA- denotes ,
T2427 3141-3144 CD denotes 1.0
T2428 3145-3147 NN denotes mM
T2429 3148-3152 NN denotes EGTA
T2430 3152-3153 -COMMA- denotes ,
T2431 3154-3157 CD denotes 1.0
T2432 3158-3160 NN denotes mM
T2433 3161-3164 NN denotes DTT
T2434 3164-3165 -COMMA- denotes ,
T2435 3166-3169 CC denotes and
T2436 3170-3173 CD denotes 1.0
T2437 3174-3176 NN denotes mM
T2438 3177-3197 NN denotes phenylmethylsulfonyl
T2439 3198-3206 NN denotes fluoride
T2440 3208-3217 JJ denotes Insoluble
T2441 3218-3226 NN denotes material
T2442 3227-3230 VB denotes was
T2443 3231-3238 VB denotes removed
T2444 3239-3241 IN denotes by
T2445 3242-3256 NN denotes centrifugation
T2446 3257-3259 IN denotes at
T2447 3260-3265 CD denotes 14000
T2448 3266-3269 NN denotes rpm
T2449 3269-3270 -COMMA- denotes ,
T2450 3271-3274 CC denotes and
T2451 3275-3278 DT denotes the
T2452 3279-3290 NN denotes supernatant
T2453 3291-3301 VB denotes containing
T2454 3302-3305 DT denotes the
T2455 3306-3313 JJ denotes nuclear
T2456 3314-3322 NN denotes proteins
T2457 3323-3326 VB denotes was
T2458 3327-3333 VB denotes stored
T2459 3334-3336 IN denotes at
T2460 3337-3342 NN denotes –80°C
T2461 3343-3348 IN denotes until
T2462 3349-3352 NN denotes use
T2463 3354-3361 NN denotes Protein
T2464 3362-3375 NN denotes concentration
T2465 3376-3379 VB denotes was
T2466 3380-3390 VB denotes determined
T2467 3391-3396 VB denotes using
T2468 3397-3398 DT denotes a
T2469 3399-3412 JJ denotes bicinchoninic
T2470 3413-3417 NN denotes acid
T2471 3418-3423 NN denotes assay
T2472 3424-3427 NN denotes kit
T2473 3428-3432 IN denotes with
T2474 3433-3439 JJ denotes bovine
T2475 3440-3445 NN denotes serum
T2476 3446-3453 NN denotes albumin
T2477 3454-3456 IN denotes as
T2478 3457-3460 DT denotes the
T2479 3461-3469 JJ denotes standard
T2480 3470-3471 -LRB- denotes (
T2481 3471-3477 NNP denotes Pierce
T2482 3478-3490 NNP denotes Biochemicals
T2483 3490-3491 -COMMA- denotes ,
T2484 3492-3500 NNP denotes Rockford
T2485 3500-3501 -COMMA- denotes ,
T2486 3502-3504 NNP denotes IL
T2487 3504-3505 -COMMA- denotes ,
T2488 3506-3509 NNP denotes USA
T2489 3509-3510 -RRB- denotes )
T2490 3512-3516 NN denotes EMSA
T2491 3517-3520 VB denotes was
T2492 3521-3530 VB denotes performed
T2493 3531-3536 VB denotes using
T2494 3537-3540 NN denotes gel
T2495 3541-3546 NN denotes shift
T2496 3547-3552 NN denotes assay
T2497 3553-3559 NN denotes system
T2498 3560-3561 -LRB- denotes (
T2499 3561-3568 NNP denotes Promega
T2500 3568-3569 -COMMA- denotes ,
T2501 3570-3577 NNP denotes Madison
T2502 3577-3578 -COMMA- denotes ,
T2503 3579-3581 NNP denotes WI
T2504 3581-3582 -COMMA- denotes ,
T2505 3583-3586 NNP denotes USA
T2506 3586-3587 -RRB- denotes )
T2507 3589-3598 NNP denotes Consensus
T2508 3599-3614 NN denotes oligonucleotide
T2509 3615-3620 NN denotes probe
T2510 3621-3622 -LRB- denotes (
T2511 3622-3649 NN denotes 5′-AGTTGAGGGGACTTTCCCAGGG-3
T2512 3649-3650 -DQE- denotes ′
T2513 3650-3651 -RRB- denotes )
T2514 3652-3655 VB denotes was
T2515 3656-3667 VB denotes end-labeled
T2516 3668-3670 IN denotes by
T2517 3671-3688 NN denotes T4-polynucleotide
T2518 3689-3695 NN denotes kinase
T2519 3696-3701 VB denotes using
T2520 3702-3703 -LRB- denotes [
T2521 3703-3708 NN denotes γ-32P
T2522 3708-3709 -RRB- denotes ]
T2523 3709-3713 NN denotes -ATP
T2524 3714-3715 -LRB- denotes (
T2525 3715-3719 NNP denotes Free
T2526 3720-3728 NNP denotes Biotech.
T2527 3728-3729 -COMMA- denotes ,
T2528 3730-3737 NNP denotes Beijing
T2529 3737-3738 -COMMA- denotes ,
T2530 3739-3744 NNP denotes China
T2531 3744-3745 -RRB- denotes )
T2532 3747-3754 JJ denotes Nuclear
T2533 3755-3762 NN denotes protein
T2534 3763-3764 -LRB- denotes (
T2535 3764-3766 CD denotes 20
T2536 3767-3769 NN denotes μg
T2537 3769-3770 -RRB- denotes )
T2538 3771-3774 VB denotes was
T2539 3775-3787 VB denotes preincubated
T2540 3788-3790 IN denotes in
T2541 3791-3793 CD denotes 20
T2542 3794-3796 NN denotes μL
T2543 3797-3804 NN denotes binding
T2544 3805-3811 NN denotes buffer
T2545 3812-3822 VB denotes containing
T2546 3823-3825 CD denotes 10
T2547 3826-3828 NN denotes mM
T2548 3829-3837 NN denotes Tris-HCl
T2549 3838-3839 -LRB- denotes (
T2550 3839-3841 NN denotes PH
T2551 3842-3845 CD denotes 7.5
T2552 3845-3846 -RRB- denotes )
T2553 3846-3847 -COMMA- denotes ,
T2554 3848-3849 CD denotes 1
T2555 3850-3852 NN denotes mM
T2556 3853-3858 NN denotes MgCl2
T2557 3858-3859 -COMMA- denotes ,
T2558 3860-3863 CD denotes 0.5
T2559 3864-3866 NN denotes mM
T2560 3867-3871 NN denotes NaCl
T2561 3871-3872 -COMMA- denotes ,
T2562 3873-3874 CD denotes 4
T2563 3874-3875 NN denotes %
T2564 3876-3884 NN denotes glycerol
T2565 3884-3885 -COMMA- denotes ,
T2566 3886-3889 CD denotes 0.5
T2567 3890-3892 NN denotes mM
T2568 3893-3897 NN denotes EDTA
T2569 3897-3898 -COMMA- denotes ,
T2570 3899-3902 CD denotes 0.5
T2571 3903-3905 NN denotes mM
T2572 3906-3909 NN denotes DTT
T2573 3909-3910 -COMMA- denotes ,
T2574 3911-3914 CC denotes and
T2575 3915-3916 CD denotes 2
T2576 3917-3919 NN denotes μg
T2577 3920-3924 NN denotes poly
T2578 3925-3930 NN denotes dI-dC
T2579 3931-3934 IN denotes for
T2580 3935-3937 CD denotes 20
T2581 3938-3945 NN denotes minutes
T2582 3946-3948 IN denotes on
T2583 3949-3952 NN denotes ice
T2584 3954-3959 IN denotes After
T2585 3960-3968 NN denotes addition
T2586 3969-3971 IN denotes of
T2587 3972-3975 DT denotes the
T2588 3976-3977 CD denotes 1
T2589 3978-3980 NN denotes μL
T2590 3981-3991 JJ denotes 32P-labled
T2591 3992-4007 NN denotes oligonucleotide
T2592 4008-4013 NN denotes probe
T2593 4013-4014 -COMMA- denotes ,
T2594 4015-4018 DT denotes the
T2595 4019-4029 NN denotes incubation
T2596 4030-4033 VB denotes was
T2597 4034-4043 VB denotes continued
T2598 4044-4047 IN denotes for
T2599 4048-4050 CD denotes 20
T2600 4051-4058 NN denotes minutes
T2601 4059-4061 IN denotes on
T2602 4062-4065 NN denotes ice
T2603 4067-4070 DT denotes The
T2604 4071-4082 JJ denotes DNA-protein
T2605 4083-4092 NN denotes complexes
T2606 4093-4097 VB denotes were
T2607 4098-4107 VB denotes separated
T2608 4108-4110 IN denotes by
T2609 4111-4126 NN denotes electrophoresis
T2610 4127-4129 IN denotes on
T2611 4130-4131 CD denotes 4
T2612 4131-4132 NN denotes %
T2613 4133-4146 JJ denotes nondenaturing
T2614 4147-4161 NN denotes polyacrylamide
T2615 4162-4165 NN denotes gel
T2616 4166-4168 IN denotes in
T2617 4169-4172 CD denotes 0.5
T2618 4173-4174 SYM denotes ×
T2619 4175-4178 NN denotes TBE
T2620 4179-4185 NN denotes buffer
T2621 4186-4187 -LRB- denotes (
T2622 4187-4203 NN denotes tris-borate-EDTA
T2623 4203-4204 -RRB- denotes )
T2624 4205-4207 IN denotes at
T2625 4208-4211 CD denotes 390
T2626 4212-4213 NN denotes V
T2627 4214-4217 IN denotes for
T2628 4218-4219 CD denotes 1
T2629 4220-4224 NN denotes hour
T2630 4225-4227 IN denotes at
T2631 4228-4231 NN denotes 4°C
T2632 4233-4238 IN denotes After
T2633 4239-4254 NN denotes electrophoresis
T2634 4254-4255 -COMMA- denotes ,
T2635 4256-4259 DT denotes the
T2636 4260-4263 NN denotes gel
T2637 4264-4267 VB denotes was
T2638 4268-4273 VB denotes dried
T2639 4274-4277 CC denotes and
T2640 4278-4285 VB denotes exposed
T2641 4286-4288 TO denotes to
T2642 4289-4294 NN denotes X-ray
T2643 4295-4299 NN denotes film
T2644 4300-4301 -LRB- denotes (
T2645 4301-4305 NNP denotes Fuji
T2646 4306-4315 NNP denotes Hyperfilm
T2647 4315-4316 -COMMA- denotes ,
T2648 4317-4322 NNP denotes Tokyo
T2649 4322-4323 -COMMA- denotes ,
T2650 4324-4329 NNP denotes Japan
T2651 4329-4330 -RRB- denotes )
T2652 4332-4338 NN denotes Levels
T2653 4339-4341 IN denotes of
T2654 4342-4347 NN denotes NF-κB
T2655 4348-4351 NN denotes DNA
T2656 4352-4359 NN denotes binding
T2657 4360-4368 NN denotes activity
T2658 4369-4373 VB denotes were
T2659 4374-4384 VB denotes quantified
T2660 4385-4387 IN denotes by
T2661 4388-4396 NN denotes software
T2662 4397-4403 NN denotes ImageJ
T3115 4406-4409 CD denotes 2.5
T3116 4411-4417 NN denotes RT-PCR
T3117 4418-4423 JJ denotes Total
T3118 4424-4427 NN denotes RNA
T3119 4428-4431 VB denotes was
T3120 4432-4440 VB denotes isolated
T3121 4441-4445 IN denotes with
T3122 4446-4452 NN denotes Trizol
T3123 4453-4454 -LRB- denotes (
T3124 4454-4464 NN denotes Invitrogen
T3125 4464-4465 -COMMA- denotes ,
T3126 4466-4468 NNP denotes CA
T3127 4468-4469 -COMMA- denotes ,
T3128 4470-4473 NNP denotes USA
T3129 4473-4474 -RRB- denotes )
T3130 4474-4475 -COMMA- denotes ,
T3131 4476-4479 CC denotes and
T3132 4480-4495 JJ denotes single-stranded
T3133 4496-4500 NN denotes cDNA
T3134 4501-4504 VB denotes was
T3135 4505-4516 VB denotes synthesized
T3136 4517-4521 IN denotes from
T3137 4522-4523 CD denotes 2
T3138 4524-4526 NN denotes μg
T3139 4527-4529 IN denotes of
T3140 4530-4535 JJ denotes total
T3141 4536-4539 NN denotes RNA
T3142 4540-4544 IN denotes with
T3143 4545-4554 JJ denotes BU-Script
T3144 4555-4561 NN denotes RT-Kit
T3145 4562-4563 -LRB- denotes (
T3146 4563-4571 NNP denotes Biunique
T3147 4571-4572 -COMMA- denotes ,
T3148 4573-4580 NNP denotes Jiangsu
T3149 4580-4581 -COMMA- denotes ,
T3150 4582-4587 NNP denotes China
T3151 4587-4588 -RRB- denotes )
T3152 4589-4598 VB denotes according
T3153 4599-4601 TO denotes to
T3154 4602-4605 DT denotes the
T3155 4606-4618 NN denotes manufacturer
T3156 4618-4620 POS denotes 's
T3157 4621-4629 NN denotes protocol
T3158 4631-4634 DT denotes The
T3159 4635-4639 NN denotes cDNA
T3160 4640-4643 VB denotes was
T3161 4644-4650 VB denotes stored
T3162 4651-4653 IN denotes in
T3163 4654-4659 NN denotes −20°C
T3164 4661-4668 JJ denotes Reverse
T3165 4669-4682 NN denotes transcription
T3166 4683-4686 VB denotes was
T3167 4687-4696 VB denotes conducted
T3168 4697-4701 IN denotes with
T3169 4702-4707 NNP denotes GoTaq
T3170 4708-4713 NNP denotes Green
T3171 4714-4720 NNP denotes Master
T3172 4721-4724 NNP denotes Mix
T3173 4725-4726 -LRB- denotes (
T3174 4726-4733 NNP denotes Promega
T3175 4733-4734 -COMMA- denotes ,
T3176 4735-4737 NNP denotes WI
T3177 4737-4738 -COMMA- denotes ,
T3178 4739-4742 NNP denotes USA
T3179 4742-4743 -RRB- denotes )
T3180 4744-4753 VB denotes according
T3181 4754-4756 TO denotes to
T3182 4757-4760 DT denotes the
T3183 4761-4773 NN denotes manufacturer
T3184 4773-4775 POS denotes 's
T3185 4776-4784 NN denotes protocol
T3186 4787-4792 JJ denotes Table
T3187 4793-4794 CD denotes 1
T3188 4795-4800 VB denotes shows
T3189 4801-4804 DT denotes the
T3190 4805-4812 NN denotes primers
T3191 4813-4816 CC denotes and
T3192 4817-4820 NN denotes PCR
T3193 4821-4831 NN denotes parameters
T3194 4833-4836 NN denotes PCR
T3195 4837-4845 NN denotes products
T3196 4846-4850 VB denotes were
T3197 4851-4859 VB denotes detected
T3198 4860-4862 IN denotes by
T3199 4863-4870 NN denotes agarose
T3200 4871-4874 NN denotes gel
T3201 4875-4890 NN denotes electrophoresis
T3202 4892-4895 DT denotes The
T3203 4896-4905 NN denotes intensity
T3204 4906-4908 IN denotes of
T3205 4909-4912 DT denotes the
T3206 4913-4918 NN denotes bands
T3207 4919-4922 VB denotes was
T3208 4923-4931 VB denotes analyzed
T3209 4932-4934 IN denotes by
T3210 4935-4941 NN denotes ImageJ
T3211 4942-4949 NN denotes program
T3212 4951-4954 DT denotes The
T3213 4955-4960 NN denotes level
T3214 4961-4963 IN denotes of
T3215 4964-4971 NN denotes β-actin
T3216 4972-4975 VB denotes was
T3217 4976-4980 VB denotes used
T3218 4981-4983 IN denotes as
T3219 4984-4986 DT denotes an
T3220 4987-4995 JJ denotes internal
T3221 4996-5004 NN denotes standard
T3389 5007-5010 CD denotes 2.6
T3390 5012-5019 NNP denotes Western
T3391 5020-5024 NNP denotes Blot
T3392 5025-5027 TO denotes To
T3393 5028-5034 VB denotes obtain
T3394 5035-5040 JJ denotes total
T3395 5041-5048 NN denotes protein
T3396 5049-5056 NN denotes lysates
T3397 5056-5057 -COMMA- denotes ,
T3398 5058-5063 NN denotes cells
T3399 5064-5068 VB denotes were
T3400 5069-5080 VB denotes homogenized
T3401 5081-5083 IN denotes in
T3402 5084-5088 NN denotes RIPA
T3403 5089-5095 NN denotes buffer
T3404 5096-5097 -LRB- denotes (
T3405 5097-5098 CD denotes 1
T3406 5098-5099 NN denotes %
T3407 5100-5104 NN denotes NP40
T3408 5104-5105 -COMMA- denotes ,
T3409 5106-5109 CD denotes 0.5
T3410 5109-5110 NN denotes %
T3411 5111-5117 NN denotes sodium
T3412 5118-5130 NN denotes deoxycholate
T3413 5130-5131 -COMMA- denotes ,
T3414 5132-5135 CD denotes 0.1
T3415 5135-5136 NN denotes %
T3416 5137-5140 NN denotes SDS
T3417 5140-5141 -COMMA- denotes ,
T3418 5142-5143 CD denotes 1
T3419 5144-5146 NN denotes mM
T3420 5147-5151 NN denotes EDTA
T3421 5151-5152 -COMMA- denotes ,
T3422 5153-5154 CD denotes 1
T3423 5155-5157 NN denotes mM
T3424 5158-5162 NN denotes EGTA
T3425 5162-5163 -COMMA- denotes ,
T3426 5164-5165 CD denotes 1
T3427 5166-5168 NN denotes mM
T3428 5169-5175 NN denotes Na3VO4
T3429 5175-5176 -COMMA- denotes ,
T3430 5177-5179 CD denotes 20
T3431 5180-5182 NN denotes mM
T3432 5183-5186 NN denotes NaF
T3433 5186-5187 -COMMA- denotes ,
T3434 5188-5191 CD denotes 0.5
T3435 5192-5194 NN denotes mM
T3436 5195-5198 NN denotes DTT
T3437 5198-5199 -COMMA- denotes ,
T3438 5200-5201 CD denotes 1
T3439 5202-5204 NN denotes mM
T3440 5205-5209 NN denotes PMSF
T3441 5209-5210 -COMMA- denotes ,
T3442 5211-5214 CC denotes and
T3443 5215-5223 NN denotes protease
T3444 5224-5233 NN denotes inhibitor
T3445 5234-5242 NN denotes cocktail
T3446 5243-5245 IN denotes in
T3447 5246-5249 NNP denotes PBS
T3448 5250-5252 NN denotes pH
T3449 5253-5256 CD denotes 7.4
T3450 5256-5257 -RRB- denotes )
T3451 5258-5261 CC denotes and
T3452 5262-5273 VB denotes centrifuged
T3453 5274-5276 IN denotes at
T3454 5277-5283 CD denotes 12,000
T3455 5284-5285 NN denotes g
T3456 5286-5289 IN denotes for
T3457 5290-5292 CD denotes 15
T3458 5293-5296 NN denotes min
T3459 5297-5299 IN denotes at
T3460 5300-5303 NN denotes 4°C
T3461 5305-5312 NN denotes Protein
T3462 5313-5327 NN denotes concentrations
T3463 5328-5332 VB denotes were
T3464 5333-5342 VB denotes estimated
T3465 5343-5345 IN denotes by
T3466 5346-5355 NNP denotes Coomassie
T3467 5356-5360 NNP denotes Plus
T3468 5361-5368 NNP denotes Protein
T3469 5369-5374 NNP denotes Assay
T3470 5375-5382 NNP denotes Reagent
T3471 5383-5384 -LRB- denotes (
T3472 5384-5390 NNP denotes Pierce
T3473 5390-5391 -COMMA- denotes ,
T3474 5392-5394 NNP denotes IL
T3475 5394-5395 -COMMA- denotes ,
T3476 5396-5399 NNP denotes USA
T3477 5399-5400 -RRB- denotes )
T3478 5402-5407 CD denotes Fifty
T3479 5408-5418 NN denotes micrograms
T3480 5419-5421 IN denotes of
T3481 5422-5425 DT denotes the
T3482 5426-5435 VB denotes resulting
T3483 5436-5445 JJ denotes cytosolic
T3484 5446-5453 NN denotes protein
T3485 5454-5462 NN denotes extracts
T3486 5463-5467 VB denotes were
T3487 5468-5482 JJ denotes heat-denatured
T3488 5483-5485 IN denotes in
T3489 5486-5493 NNP denotes Laemmli
T3490 5494-5500 NN denotes sample
T3491 5501-5508 NN denotes loading
T3492 5509-5515 NN denotes buffer
T3493 5515-5516 -COMMA- denotes ,
T3494 5517-5526 VB denotes separated
T3495 5527-5529 IN denotes by
T3496 5530-5532 CD denotes 10
T3497 5532-5533 NN denotes %
T3498 5534-5540 NN denotes sodium
T3499 5541-5548 NN denotes dodecyl
T3500 5549-5556 NN denotes sulfate
T3501 5557-5571 NN denotes polyacrylamide
T3502 5572-5575 NN denotes gel
T3503 5576-5591 NN denotes electrophoresis
T3504 5591-5592 -COMMA- denotes ,
T3505 5593-5596 CC denotes and
T3506 5597-5611 VB denotes electroblotted
T3507 5612-5616 IN denotes onto
T3508 5617-5618 DT denotes a
T3509 5619-5633 NN denotes nitrocellulose
T3510 5634-5642 NN denotes membrane
T3511 5644-5647 IN denotes For
T3512 5648-5662 NN denotes immunoblotting
T3513 5662-5663 -COMMA- denotes ,
T3514 5664-5673 NN denotes membranes
T3515 5674-5678 VB denotes were
T3516 5679-5686 VB denotes blocked
T3517 5687-5691 IN denotes with
T3518 5692-5693 CD denotes 5
T3519 5693-5694 NN denotes %
T3520 5695-5701 JJ denotes nonfat
T3521 5702-5705 JJ denotes dry
T3522 5706-5710 NN denotes milk
T3523 5711-5713 IN denotes in
T3524 5714-5720 NN denotes saline
T3525 5721-5727 NN denotes buffer
T3526 5728-5737 RB denotes overnight
T3527 5738-5740 IN denotes at
T3528 5741-5744 NN denotes 4°C
T3529 5744-5745 -COMMA- denotes ,
T3530 5746-5749 CC denotes and
T3531 5750-5753 DT denotes the
T3532 5754-5763 VB denotes following
T3533 5764-5774 NN denotes antibodies
T3534 5775-5779 VB denotes were
T3535 5780-5784 VB denotes used
T3536 5784-5785 -COLON- denotes :
T3537 5786-5798 JJ denotes anti-β-actin
T3538 5799-5800 -LRB- denotes (
T3539 5800-5809 NN denotes sc-130657
T3540 5809-5810 -COMMA- denotes ,
T3541 5811-5816 NNP denotes Santa
T3542 5817-5821 NNP denotes Cruz
T3543 5822-5835 NNP denotes Biotechnology
T3544 5835-5836 -COMMA- denotes ,
T3545 5837-5839 NNP denotes CA
T3546 5839-5840 -COMMA- denotes ,
T3547 5841-5844 NNP denotes USA
T3548 5844-5845 -COMMA- denotes ,
T3549 5846-5848 CD denotes 43
T3550 5849-5852 NN denotes kDa
T3551 5852-5853 -RRB- denotes )
T3552 5854-5857 CC denotes and
T3553 5858-5869 JJ denotes anti-matrix
T3554 5870-5886 NN denotes metallopeptidase
T3555 5887-5888 CD denotes 9
T3556 5889-5890 -LRB- denotes (
T3557 5890-5894 NN denotes MMP9
T3558 5894-5895 -RRB- denotes )
T3559 5896-5897 -LRB- denotes (
T3560 5897-5904 NN denotes sc-6841
T3561 5904-5905 -COMMA- denotes ,
T3562 5906-5911 NNP denotes Santa
T3563 5912-5916 NNP denotes Cruz
T3564 5917-5930 NNP denotes Biotechnology
T3565 5930-5931 -COMMA- denotes ,
T3566 5932-5934 NNP denotes CA
T3567 5934-5935 -COMMA- denotes ,
T3568 5936-5939 NNP denotes USA
T3569 5939-5940 -COMMA- denotes ,
T3570 5941-5943 CD denotes 92
T3571 5944-5947 NN denotes kDa
T3572 5947-5948 -RRB- denotes )
T3573 5950-5954 DT denotes Each
T3574 5955-5962 JJ denotes primary
T3575 5963-5971 NN denotes antibody
T3576 5972-5975 VB denotes was
T3577 5976-5983 VB denotes diluted
T3578 5984-5997 RB denotes appropriately
T3579 5998-6000 IN denotes in
T3580 6001-6009 VB denotes blocking
T3581 6010-6016 NN denotes buffer
T3582 6017-6020 CC denotes and
T3583 6021-6025 RB denotes then
T3584 6026-6031 VB denotes added
T3585 6032-6034 TO denotes to
T3586 6035-6038 DT denotes the
T3587 6039-6044 NN denotes blots
T3588 6045-6048 IN denotes for
T3589 6049-6050 CD denotes 1
T3590 6051-6052 NN denotes h
T3591 6053-6055 IN denotes at
T3592 6056-6060 NN denotes room
T3593 6061-6072 NN denotes temperature
T3594 6074-6077 DT denotes The
T3595 6078-6083 NN denotes blots
T3596 6084-6088 VB denotes were
T3597 6089-6095 VB denotes washed
T3598 6096-6101 CD denotes three
T3599 6102-6107 NN denotes times
T3600 6108-6110 IN denotes in
T3601 6111-6114 DT denotes the
T3602 6115-6122 VB denotes washing
T3603 6123-6129 NN denotes buffer
T3604 6130-6133 CC denotes and
T3605 6134-6141 VB denotes covered
T3606 6142-6146 IN denotes with
T3607 6147-6150 DT denotes the
T3608 6151-6162 JJ denotes horseradish
T3609 6163-6180 JJ denotes peroxidase-linked
T3610 6181-6190 JJ denotes secondary
T3611 6191-6199 NN denotes antibody
T3612 6200-6202 IN denotes at
T3613 6203-6204 DT denotes a
T3614 6205-6206 CD denotes 1
T3615 6207-6208 -COLON- denotes :
T3616 6209-6213 CD denotes 2000
T3617 6214-6222 NN denotes dilution
T3618 6223-6226 IN denotes for
T3619 6227-6228 CD denotes 1
T3620 6229-6230 NN denotes h
T3621 6232-6237 NN denotes Blots
T3622 6238-6242 VB denotes were
T3623 6243-6252 VB denotes incubated
T3624 6253-6257 IN denotes with
T3625 6258-6266 VB denotes enhanced
T3626 6267-6284 NN denotes chemiluminescence
T3627 6285-6286 -LRB- denotes (
T3628 6286-6289 NN denotes ECL
T3629 6289-6290 -RRB- denotes )
T3630 6291-6300 NN denotes detection
T3631 6301-6307 NN denotes system
T3632 6308-6309 -LRB- denotes (
T3633 6309-6317 NNP denotes Amersham
T3634 6318-6329 NNP denotes Biosciences
T3635 6329-6330 -COMMA- denotes ,
T3636 6331-6336 NNP denotes Bucks
T3637 6336-6337 -COMMA- denotes ,
T3638 6338-6340 NNP denotes UK
T3639 6340-6341 -RRB- denotes )
T3640 6342-6345 CC denotes and
T3641 6346-6353 VB denotes exposed
T3642 6354-6356 TO denotes to
T3643 6357-6369 JJ denotes radiographic
T3644 6370-6374 NN denotes film
T3645 6375-6376 -LRB- denotes (
T3646 6376-6380 NNP denotes Fuji
T3647 6381-6390 NNP denotes Hyperfilm
T3648 6390-6391 -COMMA- denotes ,
T3649 6392-6397 NNP denotes Tokyo
T3650 6397-6398 -COMMA- denotes ,
T3651 6399-6404 NNP denotes Japan
T3652 6404-6405 -RRB- denotes )
T3653 6407-6413 NN denotes ImageJ
T3654 6414-6417 VB denotes was
T3655 6418-6422 VB denotes used
T3656 6423-6425 TO denotes to
T3657 6426-6433 VB denotes analyze
T3658 6434-6437 DT denotes the
T3659 6438-6447 NN denotes intensity
T3660 6448-6450 IN denotes of
T3661 6451-6454 DT denotes the
T3662 6455-6460 NN denotes blots
T3663 6462-6465 DT denotes The
T3664 6466-6471 NN denotes level
T3665 6472-6474 IN denotes of
T3666 6475-6482 NN denotes β-actin
T3667 6483-6486 VB denotes was
T3668 6487-6491 VB denotes used
T3669 6492-6494 IN denotes as
T3670 6495-6503 JJ denotes internal
T3671 6504-6512 NN denotes standard
T4081 6515-6518 CD denotes 2.7
T4082 6520-6533 JJ denotes Enzyme-Linked
T4083 6534-6547 JJ denotes Immunosorbent
T4084 6548-6553 NN denotes Assay
T4085 6554-6555 -LRB- denotes (
T4086 6555-6560 NN denotes ELISA
T4087 6560-6561 -RRB- denotes )
T4088 6562-6567 NN denotes Cells
T4089 6568-6570 IN denotes of
T4090 6571-6575 CD denotes four
T4091 6576-6582 NN denotes groups
T4092 6583-6587 VB denotes were
T4093 6588-6599 VB denotes homogenized
T4094 6600-6602 IN denotes as
T4095 6603-6612 VB denotes mentioned
T4096 6613-6618 IN denotes above
T4097 6620-6623 DT denotes The
T4098 6624-6635 NN denotes supernatant
T4099 6636-6639 VB denotes was
T4100 6640-6649 VB denotes collected
T4101 6649-6650 -COMMA- denotes ,
T4102 6651-6654 CC denotes and
T4103 6655-6660 JJ denotes total
T4104 6661-6668 NN denotes protein
T4105 6669-6672 VB denotes was
T4106 6673-6683 VB denotes determined
T4107 6684-6686 IN denotes by
T4108 6687-6696 NNP denotes Coomassie
T4109 6697-6701 NNP denotes Plus
T4110 6702-6709 NNP denotes Protein
T4111 6710-6715 NNP denotes Assay
T4112 6716-6723 NNP denotes Reagent
T4113 6724-6725 -LRB- denotes (
T4114 6725-6731 NNP denotes Pierce
T4115 6731-6732 -COMMA- denotes ,
T4116 6733-6735 NNP denotes IL
T4117 6735-6736 -COMMA- denotes ,
T4118 6737-6740 NNP denotes USA
T4119 6740-6741 -RRB- denotes )
T4120 6743-6749 NN denotes Levels
T4121 6750-6752 IN denotes of
T4122 6753-6758 NN denotes tumor
T4123 6759-6767 NN denotes necrosis
T4124 6768-6780 NN denotes factor-alpha
T4125 6781-6782 -LRB- denotes (
T4126 6782-6787 NN denotes TNF-α
T4127 6787-6788 -RRB- denotes )
T4128 6788-6789 -COMMA- denotes ,
T4129 6790-6803 NN denotes interleukin-1
T4130 6804-6808 NN denotes beta
T4131 6809-6810 -LRB- denotes (
T4132 6810-6815 NN denotes IL-1β
T4133 6815-6816 -RRB- denotes )
T4134 6816-6817 -COMMA- denotes ,
T4135 6818-6821 CC denotes and
T4136 6822-6835 NN denotes interleukin-6
T4137 6836-6837 -LRB- denotes (
T4138 6837-6841 NN denotes IL-6
T4139 6841-6842 -RRB- denotes )
T4140 6843-6850 NN denotes protein
T4141 6851-6855 VB denotes were
T4142 6856-6866 VB denotes quantified
T4143 6867-6872 VB denotes using
T4144 6873-6878 NN denotes ELISA
T4145 6879-6883 NN denotes kits
T4146 6884-6892 JJ denotes specific
T4147 6893-6896 IN denotes for
T4148 6897-6902 NN denotes mouse
T4149 6903-6912 VB denotes according
T4150 6913-6915 TO denotes to
T4151 6916-6919 DT denotes the
T4152 6920-6932 NN denotes manufacturer
T4153 6932-6934 POS denotes 's
T4154 6935-6947 NN denotes instructions
T4155 6948-6949 -LRB- denotes (
T4156 6949-6955 NNP denotes Bender
T4157 6956-6966 NNP denotes MedSystems
T4158 6967-6971 NNP denotes Inc.
T4159 6972-6974 NNP denotes CA
T4160 6974-6975 -COMMA- denotes ,
T4161 6976-6979 NNP denotes USA
T4162 6979-6980 -RRB- denotes )
T4163 6982-6989 NNP denotes Briefly
T4164 6989-6990 -COMMA- denotes ,
T4165 6991-6999 VB denotes prepared
T4166 7000-7003 DT denotes the
T4167 7004-7012 NN denotes standard
T4168 7013-7016 CC denotes and
T4169 7017-7024 VB denotes created
T4170 7025-7028 DT denotes the
T4171 7029-7037 JJ denotes standard
T4172 7038-7046 NN denotes dilution
T4173 7047-7050 IN denotes for
T4174 7051-7059 VB denotes building
T4175 7060-7068 JJ denotes standard
T4176 7069-7074 NN denotes curve
T4177 7076-7080 RB denotes Then
T4178 7081-7088 NN denotes samples
T4179 7089-7092 CC denotes and
T4180 7093-7108 NN denotes biotinconjugate
T4181 7109-7113 VB denotes were
T4182 7114-7119 VB denotes added
T4183 7120-7122 TO denotes to
T4184 7123-7132 VB denotes microwell
T4185 7133-7139 NN denotes strips
T4186 7141-7146 IN denotes After
T4187 7147-7156 VB denotes incubated
T4188 7157-7160 IN denotes for
T4189 7161-7162 CD denotes 2
T4190 7163-7164 NN denotes h
T4191 7165-7167 IN denotes at
T4192 7168-7172 NN denotes room
T4193 7173-7184 NN denotes temperature
T4194 7184-7185 -COMMA- denotes ,
T4195 7186-7189 DT denotes the
T4196 7190-7199 NN denotes microwell
T4197 7200-7206 NN denotes strips
T4198 7207-7211 VB denotes were
T4199 7212-7218 VB denotes washed
T4200 7219-7220 CD denotes 3
T4201 7221-7226 NN denotes times
T4202 7227-7231 IN denotes with
T4203 7232-7236 NN denotes wash
T4204 7237-7243 NN denotes buffer
T4205 7243-7244 -COMMA- denotes ,
T4206 7245-7248 CC denotes and
T4207 7249-7265 NN denotes streptavidin-HRP
T4208 7266-7270 VB denotes were
T4209 7271-7276 VB denotes added
T4210 7277-7279 TO denotes to
T4211 7280-7283 DT denotes all
T4212 7284-7289 NN denotes wells
T4213 7291-7296 IN denotes After
T4214 7297-7306 VB denotes incubated
T4215 7307-7310 IN denotes for
T4216 7311-7312 CD denotes 1
T4217 7313-7314 NN denotes h
T4218 7314-7315 -COMMA- denotes ,
T4219 7316-7325 NN denotes microwell
T4220 7326-7332 NN denotes strips
T4221 7333-7337 VB denotes were
T4222 7338-7344 VB denotes washed
T4223 7345-7346 CD denotes 3
T4224 7347-7352 NN denotes times
T4225 7353-7361 VB denotes followed
T4226 7362-7364 IN denotes by
T4227 7365-7371 VB denotes adding
T4228 7372-7375 NN denotes TMB
T4229 7376-7385 NN denotes substrate
T4230 7387-7392 IN denotes After
T4231 7393-7402 VB denotes incubated
T4232 7403-7406 IN denotes for
T4233 7407-7412 RB denotes about
T4234 7413-7418 CD denotes 10–30
T4235 7419-7422 NN denotes min
T4236 7422-7423 -COMMA- denotes ,
T4237 7424-7427 DT denotes the
T4238 7428-7432 NN denotes stop
T4239 7433-7441 NN denotes solution
T4240 7442-7445 VB denotes was
T4241 7446-7451 VB denotes added
T4242 7453-7456 DT denotes The
T4243 7457-7463 NN denotes colour
T4244 7464-7473 NN denotes intensity
T4245 7474-7477 VB denotes was
T4246 7478-7486 VB denotes measured
T4247 7487-7489 IN denotes at
T4248 7490-7493 CD denotes 450
T4249 7494-7496 NN denotes nm
T4250 7497-7502 VB denotes using
T4251 7503-7504 DT denotes a
T4252 7505-7512 JJ denotes Bio-Rad
T4253 7513-7518 NN denotes ELISA
T4254 7519-7529 NN denotes microplate
T4255 7530-7536 NN denotes reader
T4256 7537-7538 -LRB- denotes (
T4257 7538-7545 NNP denotes Bio-Rad
T4258 7546-7558 NNP denotes Laboratories
T4259 7558-7559 -COMMA- denotes ,
T4260 7560-7562 NNP denotes CA
T4261 7562-7563 -COMMA- denotes ,
T4262 7564-7567 NNP denotes USA
T4263 7567-7568 -RRB- denotes )
T4264 7570-7573 DT denotes The
T4265 7574-7587 NN denotes concentration
T4266 7588-7590 IN denotes of
T4267 7591-7598 NN denotes protein
T4268 7599-7602 VB denotes was
T4269 7603-7613 VB denotes determined
T4270 7614-7623 VB denotes according
T4271 7624-7626 TO denotes to
T4272 7627-7630 DT denotes the
T4273 7631-7639 JJ denotes standard
T4274 7640-7645 NN denotes curve
T4275 7646-7649 CC denotes and
T4276 7650-7659 VB denotes expressed
T4277 7660-7662 IN denotes as
T4278 7663-7668 NN denotes pg/mg
T4279 7669-7671 IN denotes of
T4280 7672-7677 JJ denotes total
T4281 7678-7685 NN denotes protein
T4392 7688-7691 CD denotes 2.8
T4393 7693-7701 NN denotes Gelatine
T4394 7702-7712 NN denotes Zymography
T4395 7713-7718 NN denotes Cells
T4396 7719-7721 IN denotes of
T4397 7722-7726 CD denotes four
T4398 7727-7733 NN denotes groups
T4399 7734-7738 VB denotes were
T4400 7739-7750 VB denotes homogenized
T4401 7751-7753 IN denotes in
T4402 7754-7759 NN denotes lysis
T4403 7760-7766 NN denotes buffer
T4404 7767-7777 VB denotes containing
T4405 7778-7780 CD denotes 50
T4406 7781-7783 NN denotes mM
T4407 7784-7792 NN denotes Tris-HCl
T4408 7793-7794 -LRB- denotes (
T4409 7794-7796 NN denotes pH
T4410 7797-7800 CD denotes 7.4
T4411 7800-7801 -RRB- denotes )
T4412 7801-7802 -COMMA- denotes ,
T4413 7803-7806 CD denotes 150
T4414 7807-7809 NN denotes mM
T4415 7810-7814 NN denotes NaCl
T4416 7814-7815 -COMMA- denotes ,
T4417 7816-7817 CD denotes 5
T4418 7818-7820 NN denotes mM
T4419 7821-7826 NN denotes CaCl2
T4420 7826-7827 -COMMA- denotes ,
T4421 7828-7831 CD denotes 0.2
T4422 7832-7834 NN denotes mM
T4423 7835-7839 NN denotes NaN3
T4424 7839-7840 -COMMA- denotes ,
T4425 7841-7844 CC denotes and
T4426 7845-7849 CD denotes 0.01
T4427 7849-7850 NN denotes %
T4428 7851-7858 NNP denotes Triton.
T4429 7859-7866 NNP denotes Soluble
T4430 7867-7875 NN denotes extracts
T4431 7876-7880 VB denotes were
T4432 7881-7890 VB denotes separated
T4433 7891-7893 IN denotes by
T4434 7894-7908 NN denotes centrifugation
T4435 7909-7912 CC denotes and
T4436 7913-7919 VB denotes stored
T4437 7920-7922 IN denotes at
T4438 7923-7928 NN denotes –20°C
T4439 7930-7937 NN denotes Gelatin
T4440 7938-7948 NN denotes zymography
T4441 7949-7952 VB denotes was
T4442 7953-7962 VB denotes performed
T4443 7963-7972 VB denotes according
T4444 7973-7975 TO denotes to
T4445 7976-7979 DT denotes the
T4446 7980-7992 NN denotes manufacturer
T4447 7992-7994 POS denotes 's
T4448 7995-8007 NN denotes instructions
T4449 8008-8009 -LRB- denotes (
T4450 8009-8015 NNP denotes Genmed
T4451 8016-8027 NNP denotes Scientifics
T4452 8028-8031 NNP denotes Inc
T4453 8031-8032 -COMMA- denotes ,
T4454 8033-8035 NNP denotes MA
T4455 8035-8036 -COMMA- denotes ,
T4456 8037-8040 NNP denotes USA
T4457 8040-8041 -RRB- denotes )
T4458 8043-8050 RB denotes Briefly
T4459 8050-8051 -COMMA- denotes ,
T4460 8052-8054 CD denotes 40
T4461 8055-8057 NN denotes μg
T4462 8058-8067 JJ denotes cytosolic
T4463 8068-8075 NN denotes protein
T4464 8076-8084 NN denotes extracts
T4465 8085-8089 VB denotes were
T4466 8090-8099 VB denotes separated
T4467 8100-8102 IN denotes by
T4468 8103-8118 NN denotes electrophoresis
T4469 8120-8124 RB denotes Then
T4470 8125-8128 DT denotes the
T4471 8129-8137 NN denotes proteins
T4472 8138-8142 VB denotes were
T4473 8143-8152 VB denotes renatured
T4474 8153-8155 IN denotes by
T4475 8156-8166 NN denotes incubation
T4476 8167-8169 IN denotes in
T4477 8170-8173 CD denotes 2.5
T4478 8173-8174 NN denotes %
T4479 8175-8181 NN denotes Triton
T4480 8182-8187 NN denotes X-100
T4481 8188-8191 CC denotes and
T4482 8192-8196 RB denotes then
T4483 8197-8206 VB denotes incubated
T4484 8207-8209 IN denotes in
T4485 8210-8219 NN denotes substrate
T4486 8220-8226 NN denotes buffer
T4487 8227-8230 IN denotes for
T4488 8231-8233 CD denotes 40
T4489 8234-8235 NN denotes h
T4490 8236-8238 IN denotes at
T4491 8239-8243 NN denotes 37°C
T4492 8244-8246 TO denotes to
T4493 8247-8253 VB denotes enable
T4494 8254-8257 DT denotes the
T4495 8258-8262 NN denotes MMP9
T4496 8263-8265 TO denotes to
T4497 8266-8272 VB denotes cleave
T4498 8273-8276 DT denotes the
T4499 8277-8284 NN denotes gelatin
T4500 8286-8291 IN denotes After
T4501 8292-8299 VB denotes rinsing
T4502 8300-8302 IN denotes in
T4503 8303-8308 NN denotes water
T4504 8308-8309 -COMMA- denotes ,
T4505 8310-8314 DT denotes each
T4506 8315-8318 NN denotes gel
T4507 8319-8322 VB denotes was
T4508 8323-8330 VB denotes stained
T4509 8331-8335 IN denotes with
T4510 8336-8345 NNP denotes Coomassie
T4511 8346-8350 JJ denotes blue
T4512 8351-8354 IN denotes for
T4513 8355-8356 CD denotes 1
T4514 8357-8358 NN denotes h
T4515 8359-8362 CC denotes and
T4516 8363-8372 VB denotes destained
T4517 8373-8375 IN denotes in
T4518 8376-8378 CD denotes 50
T4519 8378-8379 NN denotes %
T4520 8380-8388 NN denotes methanol
T4521 8390-8401 JJ denotes Proteolytic
T4522 8402-8412 NN denotes activities
T4523 8413-8417 VB denotes were
T4524 8418-8424 VB denotes showed
T4525 8425-8427 IN denotes by
T4526 8428-8433 JJ denotes clear
T4527 8434-8439 NN denotes bands
T4528 8440-8442 IN denotes in
T4529 8443-8447 JJ denotes blue
T4530 8448-8451 NN denotes gel
T4531 8452-8457 WDT denotes which
T4532 8458-8467 VB denotes indicates
T4533 8468-8471 DT denotes the
T4534 8472-8477 NN denotes lysis
T4535 8478-8480 IN denotes of
T4536 8481-8484 DT denotes the
T4537 8485-8494 NN denotes substrate
T4538 8496-8510 NN denotes Quantification
T4539 8511-8513 IN denotes of
T4540 8514-8518 NN denotes MMP9
T4541 8519-8523 NN denotes band
T4542 8524-8531 NN denotes density
T4543 8532-8535 VB denotes was
T4544 8536-8545 VB denotes performed
T4545 8546-8550 IN denotes with
T4546 8551-8556 NN denotes image
T4547 8557-8565 NN denotes analysis
T4548 8566-8573 NN denotes program
T4549 8574-8580 NN denotes ImageJ
T4762 8583-8586 CD denotes 2.9
T4763 8588-8599 JJ denotes Statistical
T4764 8600-8608 NN denotes Analysis
T4765 8609-8613 NN denotes Data
T4766 8614-8618 VB denotes were
T4767 8619-8628 VB denotes expressed
T4768 8629-8631 IN denotes as
T4769 8632-8636 NN denotes mean
T4770 8637-8638 SYM denotes ±
T4771 8639-8641 NN denotes SD
T4772 8642-8645 CC denotes and
T4773 8646-8655 VB denotes evaluated
T4774 8656-8658 IN denotes by
T4775 8659-8664 NN denotes ANOVA
T4776 8665-8668 CC denotes and
T4777 8669-8672 NN denotes LSD
T4778 8673-8681 JJ denotes multiple
T4779 8682-8692 NN denotes comparison
T4780 8693-8697 NN denotes test
T4781 8699-8700 NN denotes P
T4782 8701-8707 NN denotes values
T4783 8708-8709 SYM denotes <
T4784 8709-8713 CD denotes 0.05
T4785 8714-8718 VB denotes were
T4786 8719-8729 VB denotes considered
T4787 8730-8732 TO denotes to
T4788 8733-8735 VB denotes be
T4789 8736-8747 JJ denotes significant
T4790 8749-8752 DT denotes All
T4791 8753-8761 NN denotes analyses
T4792 8762-8766 VB denotes were
T4793 8767-8776 VB denotes performed
T4794 8777-8779 IN denotes by
T4795 8780-8785 VB denotes using
T4796 8786-8790 NN denotes SPSS
T4797 8791-8795 CD denotes 18.0
T4798 8796-8804 NN denotes software
R1000 T1259 T1256 arg1Of Kensler,Dr.
R1001 T1259 T1257 arg1Of Kensler,Thomas
R1002 T1259 T1258 arg1Of Kensler,W.
R1003 T1259 T1260 arg1Of Kensler,(
R1004 T1263 T1260 arg2Of University,(
R1005 T1263 T1261 arg1Of University,Johns
R1006 T1263 T1262 arg1Of University,Hopkins
R1007 T1263 T1264 arg1Of University,","
R1008 T1263 T1268 arg1Of University,","
R1009 T1263 T1269 arg1Of University,USA
R1010 T1265 T1264 arg2Of Baltimore,","
R1011 T1265 T1266 arg1Of Baltimore,","
R1012 T1267 T1266 arg2Of MD,","
R1013 T1270 T1260 arg3Of ),(
R1014 T1272 T1271 arg1Of astrocytes,Primary
R1015 T1272 T1273 arg1Of astrocytes,were
R1016 T1272 T1274 arg2Of astrocytes,obtained
R1017 T1274 T1273 arg2Of obtained,were
R1018 T1274 T1275 arg1Of obtained,from
R1019 T1280 T1276 arg1Of type,postnatal
R1020 T1280 T1277 arg1Of type,2-day-old
R1021 T1280 T1278 arg1Of type,Nrf2
R1022 T1280 T1279 arg1Of type,wide
R1023 T1280 T1281 arg1Of type,(
R1024 T1280 T1284 arg1Of type,and
R1025 T1282 T1281 arg2Of WT,(
R1026 T1283 T1281 arg3Of ),(
R1027 T1284 T1275 arg2Of and,from
R1028 T1287 T1286 arg2Of KO,(
R1029 T1288 T1286 arg3Of ),(
R1030 T1289 T1284 arg2Of mice,and
R1031 T1289 T1285 arg1Of mice,knockout
R1032 T1289 T1286 arg1Of mice,(
R1033 T1289 T1290 arg1Of mice,(
R1034 T1292 T1290 arg2Of mice,(
R1035 T1292 T1291 arg1Of mice,6
R1036 T1292 T1293 arg1Of mice,for
R1037 T1295 T1293 arg2Of genotype,for
R1038 T1295 T1294 arg1Of genotype,each
R1039 T1296 T1290 arg3Of ),(
R1040 T1298 T1297 arg2Of decapitation,Following
R1041 T1301 T1300 arg1Of cortices,the
R1042 T1301 T1302 arg1Of cortices,were
R1043 T1301 T1303 arg2Of cortices,dissected
R1044 T1303 T1302 arg2Of dissected,were
R1045 T1303 T1304 arg1Of dissected,out
R1046 T1303 T1306 arg1Of dissected,and
R1047 T1306 T1297 arg1Of and,Following
R1048 T1306 T1299 arg1Of and,","
R1049 T1306 T1305 arg1Of and,","
R1050 T1308 T1309 arg1Of meninges,and
R1051 T1309 T1307 arg1Of and,the
R1052 T1309 T1313 arg1Of and,were
R1053 T1309 T1314 arg2Of and,removed
R1054 T1312 T1309 arg2Of vessels,and
R1055 T1312 T1310 arg2Of vessels,associated
R1056 T1312 T1311 arg1Of vessels,blood
R1057 T1314 T1306 arg2Of removed,and
R1058 T1314 T1313 arg2Of removed,were
R1059 T1316 T1315 arg1Of tissue,The
R1060 T1316 T1317 arg1Of tissue,was
R1061 T1316 T1319 arg2Of tissue,chopped
R1062 T1316 T1337 arg1Of tissue,agitated
R1063 T1319 T1317 arg2Of chopped,was
R1064 T1319 T1318 arg1Of chopped,roughly
R1065 T1319 T1320 arg1Of chopped,with
R1066 T1319 T1336 arg1Of chopped,and
R1067 T1323 T1320 arg2Of blade,with
R1068 T1323 T1321 arg1Of blade,a
R1069 T1323 T1322 arg1Of blade,scalpel
R1070 T1323 T1324 arg1Of blade,","
R1071 T1323 T1325 arg2Of blade,incubated
R1072 T1325 T1326 arg1Of incubated,in
R1073 T1327 T1328 arg1Of 0.5,%
R1074 T1329 T1326 arg2Of trypsin,in
R1075 T1329 T1327 arg1Of trypsin,0.5
R1076 T1329 T1330 arg1Of trypsin,for
R1077 T1329 T1333 arg1Of trypsin,at
R1078 T1332 T1330 arg2Of minutes,for
R1079 T1332 T1331 arg1Of minutes,10
R1080 T1334 T1333 arg2Of 37°C,at
R1081 T1336 T1335 arg1Of and,","
R1082 T1337 T1336 arg2Of agitated,and
R1083 T1340 T1337 arg2Of minutes,agitated
R1084 T1340 T1338 arg1Of minutes,every
R1085 T1340 T1339 arg1Of minutes,few
R1086 T1342 T1341 arg2Of digestion,After
R1087 T1345 T1344 arg1Of tissue,the
R1088 T1345 T1346 arg1Of tissue,was
R1089 T1345 T1347 arg2Of tissue,rinsed
R1090 T1347 T1341 arg1Of rinsed,After
R1091 T1347 T1343 arg1Of rinsed,","
R1092 T1347 T1346 arg2Of rinsed,was
R1093 T1347 T1348 arg1Of rinsed,twice
R1094 T1347 T1349 arg1Of rinsed,in
R1095 T1350 T1349 arg2Of DMEM,in
R1096 T1350 T1351 arg1Of DMEM,with
R1097 T1352 T1353 arg1Of 10,%
R1098 T1354 T1352 arg1Of FBS,10
R1099 T1354 T1355 arg1Of FBS,","
R1100 T1354 T1356 arg2Of FBS,followed
R1101 T1354 T1371 arg1Of FBS,","
R1102 T1360 T1356 arg1Of dissociation,followed
R1103 T1360 T1357 arg2Of dissociation,by
R1104 T1360 T1358 arg1Of dissociation,a
R1105 T1360 T1359 arg1Of dissociation,mechanical
R1106 T1360 T1361 arg1Of dissociation,in
R1107 T1362 T1361 arg2Of DMEM,in
R1108 T1362 T1363 arg1Of DMEM,with
R1109 T1366 T1364 arg1Of FBS,20
R1110 T1366 T1365 arg1Of FBS,%
R1111 T1366 T1367 arg1Of FBS,and
R1112 T1367 T1363 arg2Of and,with
R1113 T1370 T1367 arg2Of penicillin,and
R1114 T1370 T1368 arg1Of penicillin,5
R1115 T1370 T1369 arg1Of penicillin,units/mL
R1116 T1371 T1351 arg2Of ",",with
R1117 T1374 T1371 arg2Of streptomycin,","
R1118 T1374 T1372 arg1Of streptomycin,5
R1119 T1374 T1373 arg1Of streptomycin,μg/mL
R1120 T1374 T1375 arg1Of streptomycin,(
R1121 T1378 T1375 arg2Of medium,(
R1122 T1378 T1376 arg1Of medium,complete
R1123 T1378 T1377 arg1Of medium,culture
R1124 T1379 T1375 arg3Of ),(
R1125 T1381 T1380 arg2Of incubation,After
R1126 T1381 T1382 arg1Of incubation,for
R1127 T1384 T1382 arg2Of h,for
R1128 T1384 T1383 arg1Of h,1
R1129 T1387 T1386 arg1Of supernatant,the
R1130 T1387 T1388 arg1Of supernatant,was
R1131 T1387 T1389 arg2Of supernatant,transferred
R1132 T1389 T1380 arg1Of transferred,After
R1133 T1389 T1385 arg1Of transferred,","
R1134 T1389 T1388 arg2Of transferred,was
R1135 T1389 T1390 arg1Of transferred,to
R1136 T1389 T1396 arg1Of transferred,(
R1137 T1389 T1401 arg1Of transferred,for
R1138 T1393 T1394 arg1Of flask,or
R1139 T1394 T1390 arg2Of or,to
R1140 T1394 T1391 arg1Of or,a
R1141 T1394 T1392 arg1Of or,new
R1142 T1395 T1394 arg2Of dish,or
R1143 T1397 T1396 arg2Of Costar,(
R1144 T1397 T1398 arg1Of Costar,","
R1145 T1397 T1399 arg1Of Costar,USA
R1146 T1400 T1396 arg3Of ),(
R1147 T1402 T1401 arg2Of depleting,for
R1148 T1406 T1402 arg2Of cells,depleting
R1149 T1406 T1403 arg1Of cells,the
R1150 T1406 T1404 arg1Of cells,residual
R1151 T1406 T1405 arg1Of cells,epithelial
R1152 T1409 T1408 arg1Of cells,the
R1153 T1409 T1410 arg1Of cells,were
R1154 T1409 T1411 arg2Of cells,cultivated
R1155 T1411 T1407 arg1Of cultivated,Then
R1156 T1411 T1410 arg2Of cultivated,were
R1157 T1411 T1412 arg1Of cultivated,at
R1158 T1413 T1412 arg2Of 37°C,at
R1159 T1413 T1414 arg1Of 37°C,with
R1160 T1415 T1416 arg1Of 5,%
R1161 T1417 T1414 arg2Of CO2,with
R1162 T1417 T1415 arg1Of CO2,5
R1163 T1422 T1419 arg1Of medium,the
R1164 T1422 T1420 arg1Of medium,complete
R1165 T1422 T1421 arg1Of medium,culture
R1166 T1422 T1423 arg1Of medium,was
R1167 T1422 T1424 arg1Of medium,half-changed
R1168 T1423 T1418 arg1Of was,And
R1169 T1423 T1425 arg1Of was,twice
R1170 T1423 T1427 arg1Of was,week
R1171 T1424 T1423 arg2Of half-changed,was
R1172 T1427 T1426 arg1Of week,a
R1173 T1428 T1429 arg2Of Astrocytes,expanded
R1174 T1428 T1435 arg1Of Astrocytes,reach
R1175 T1429 T1430 arg1Of expanded,for
R1176 T1429 T1434 modOf expanded,to
R1177 T1432 T1431 arg1Of 7,about
R1178 T1433 T1430 arg2Of days,for
R1179 T1433 T1432 arg1Of days,7
R1180 T1435 T1434 arg1Of reach,to
R1181 T1436 T1435 arg2Of confluence,reach
R1182 T1439 T1440 arg1Of flasks,or
R1183 T1440 T1438 arg1Of or,the
R1184 T1440 T1442 arg1Of or,were
R1185 T1440 T1443 arg2Of or,shaken
R1186 T1441 T1440 arg2Of dishes,or
R1187 T1443 T1437 arg1Of shaken,Then
R1188 T1443 T1442 arg2Of shaken,were
R1189 T1443 T1444 arg1Of shaken,at
R1190 T1446 T1444 arg2Of rpm,at
R1191 T1446 T1445 arg1Of rpm,150
R1192 T1446 T1447 arg1Of rpm,for
R1193 T1449 T1447 arg2Of h,for
R1194 T1449 T1448 arg1Of h,4
R1195 T1449 T1451 arg1Of h,deplete
R1196 T1451 T1447 arg3Of deplete,for
R1197 T1451 T1450 arg1Of deplete,to
R1198 T1451 T1458 arg1Of deplete,from
R1199 T1453 T1454 arg1Of microglia,and
R1200 T1454 T1451 arg2Of and,deplete
R1201 T1454 T1452 arg1Of and,the
R1202 T1456 T1455 arg1Of adherent,less
R1203 T1457 T1454 arg2Of cells,and
R1204 T1457 T1456 arg1Of cells,adherent
R1205 T1460 T1458 arg2Of cultures,from
R1206 T1460 T1459 arg1Of cultures,the
R1207 T1462 T1461 arg2Of shaking,After
R1208 T1466 T1462 arg1Of cultures,shaking
R1209 T1466 T1464 arg1Of cultures,the
R1210 T1466 T1465 arg1Of cultures,resulting
R1211 T1466 T1467 arg1Of cultures,were
R1212 T1467 T1461 arg1Of were,After
R1213 T1467 T1463 arg1Of were,","
R1214 T1469 T1467 arg2Of astrocytes,were
R1215 T1469 T1468 arg1Of astrocytes,mainly
R1216 T1469 T1470 arg1Of astrocytes,","
R1217 T1469 T1471 arg1Of astrocytes,which
R1218 T1469 T1472 arg1Of astrocytes,were
R1219 T1469 T1473 arg2Of astrocytes,determined
R1220 T1473 T1472 arg2Of determined,were
R1221 T1473 T1474 arg1Of determined,by
R1222 T1475 T1474 arg2Of immunoreactivity,by
R1223 T1475 T1476 arg1Of immunoreactivity,for
R1224 T1477 T1476 arg2Of GFAP,for
R1225 T1477 T1478 arg1Of GFAP,(
R1226 T1479 T1480 arg1Of sc-166481,","
R1227 T1479 T1484 arg1Of sc-166481,","
R1228 T1483 T1480 arg2Of Biotechnology,","
R1229 T1483 T1481 arg1Of Biotechnology,Santa
R1230 T1483 T1482 arg1Of Biotechnology,Cruz
R1231 T1484 T1478 arg2Of ",",(
R1232 T1485 T1484 arg2Of CA,","
R1233 T1485 T1486 arg1Of CA,","
R1234 T1487 T1486 arg2Of USA,","
R1235 T1488 T1478 arg3Of ),(
R1236 T1489 T1490 arg2Of Cells,passaged
R1237 T1489 T1494 arg1Of Cells,were
R1238 T1489 T1495 arg2Of Cells,used
R1239 T1490 T1491 arg1Of passaged,for
R1240 T1493 T1491 arg2Of generations,for
R1241 T1493 T1492 arg1Of generations,2-3
R1242 T1495 T1494 arg2Of used,were
R1243 T1495 T1496 arg1Of used,in
R1244 T1499 T1496 arg2Of studies,in
R1245 T1499 T1497 arg1Of studies,the
R1246 T1499 T1498 arg1Of studies,following
R1526 T1824 T1822 arg2Of Model,In
R1527 T1824 T1823 arg1Of Model,Vitro
R1528 T1824 T1825 arg1Of Model,of
R1529 T1824 T1827 arg2Of Model,Established
R1530 T1826 T1825 arg2Of TBI,of
R1531 T1830 T1827 arg1Of Injury,Established
R1532 T1830 T1828 arg2Of Injury,by
R1533 T1830 T1829 arg1Of Injury,Scratch
R1534 T1833 T1831 arg1Of injury,Astrocyte
R1535 T1833 T1832 arg1Of injury,scratch
R1536 T1833 T1834 arg1Of injury,was
R1537 T1833 T1835 arg2Of injury,performed
R1538 T1835 T1834 arg2Of performed,was
R1539 T1835 T1837 arg1Of performed,in
R1540 T1835 T1841 arg1Of performed,[
R1541 T1837 T1836 arg1Of in,as
R1542 T1840 T1837 arg2Of report,in
R1543 T1840 T1838 arg1Of report,a
R1544 T1840 T1839 arg1Of report,previous
R1545 T1842 T1841 arg2Of 7,[
R1546 T1843 T1841 arg3Of ],[
R1547 T1844 T1845 arg1Of Astrocytes,were
R1548 T1844 T1846 arg2Of Astrocytes,planted
R1549 T1844 T1851 arg2Of Astrocytes,grown
R1550 T1846 T1847 arg1Of planted,in
R1551 T1846 T1850 arg1Of planted,and
R1552 T1849 T1847 arg2Of plates,in
R1553 T1849 T1848 arg1Of plates,6-well
R1554 T1850 T1845 arg2Of and,were
R1555 T1850 T1852 modOf and,to
R1556 T1851 T1850 arg2Of grown,and
R1557 T1853 T1852 arg1Of confluence,to
R1558 T1856 T1854 arg1Of monolayer,The
R1559 T1856 T1855 arg1Of monolayer,cell
R1560 T1856 T1857 arg1Of monolayer,was
R1561 T1856 T1858 arg2Of monolayer,scratched
R1562 T1858 T1857 arg2Of scratched,was
R1563 T1858 T1859 arg1Of scratched,with
R1564 T1858 T1865 arg1Of scratched,","
R1565 T1858 T1866 modOf scratched,resulting
R1566 T1864 T1859 arg2Of needle,with
R1567 T1864 T1860 arg1Of needle,a
R1568 T1864 T1861 arg1Of needle,sterile
R1569 T1864 T1862 arg1Of needle,26G
R1570 T1864 T1863 arg1Of needle,syringe
R1571 T1866 T1867 arg1Of resulting,in
R1572 T1869 T1867 arg2Of formation,in
R1573 T1869 T1868 arg1Of formation,the
R1574 T1869 T1870 arg1Of formation,of
R1575 T1875 T1870 arg2Of gap,of
R1576 T1875 T1871 arg1Of gap,a
R1577 T1875 T1872 arg1Of gap,0.5
R1578 T1875 T1873 arg1Of gap,mm
R1579 T1875 T1874 arg1Of gap,wide
R1580 T1877 T1876 arg1Of after,Immediately
R1581 T1878 T1877 arg2Of scratch,after
R1582 T1880 T1881 arg1Of cells,were
R1583 T1880 T1882 arg2Of cells,washed
R1584 T1880 T1895 arg2Of cells,named
R1585 T1882 T1883 arg1Of washed,twice
R1586 T1882 T1884 arg1Of washed,with
R1587 T1882 T1894 arg1Of washed,and
R1588 T1886 T1884 arg2Of PBS,with
R1589 T1886 T1885 arg1Of PBS,sterile
R1590 T1886 T1887 arg1Of PBS,","
R1591 T1886 T1888 arg2Of PBS,cultured
R1592 T1888 T1889 arg1Of cultured,with
R1593 T1892 T1889 arg2Of medium,with
R1594 T1892 T1890 arg1Of medium,complete
R1595 T1892 T1891 arg1Of medium,culture
R1596 T1894 T1877 arg1Of and,after
R1597 T1894 T1879 arg1Of and,","
R1598 T1894 T1881 arg2Of and,were
R1599 T1894 T1893 arg1Of and,","
R1600 T1895 T1894 arg2Of named,and
R1601 T1895 T1896 arg1Of named,as
R1602 T1898 T1896 arg2Of group,as
R1603 T1898 T1897 arg1Of group,TBI
R1604 T1899 T1900 arg1Of Cells,","
R1605 T1899 T1901 arg1Of Cells,which
R1606 T1899 T1902 arg1Of Cells,did
R1607 T1899 T1907 arg1Of Cells,were
R1608 T1899 T1908 arg2Of Cells,used
R1609 T1899 T1913 arg2Of Cells,named
R1610 T1902 T1903 arg1Of did,not
R1611 T1905 T1902 arg2Of scratch,did
R1612 T1905 T1904 arg2Of scratch,received
R1613 T1908 T1909 arg1Of used,as
R1614 T1908 T1912 arg1Of used,and
R1615 T1911 T1909 arg2Of control,as
R1616 T1911 T1910 arg1Of control,blank
R1617 T1912 T1906 arg1Of and,","
R1618 T1912 T1907 arg2Of and,were
R1619 T1913 T1912 arg2Of named,and
R1620 T1913 T1914 arg1Of named,as
R1621 T1916 T1914 arg2Of group,as
R1622 T1916 T1915 arg1Of group,sham
R1726 T2050 T2048 arg1Of Measurement,Cell
R1727 T2050 T2049 arg1Of Measurement,Death
R1728 T2050 T2051 arg1Of Measurement,by
R1729 T2053 T2051 arg2Of Assay,by
R1730 T2053 T2052 arg1Of Assay,CCK-8
R1731 T2057 T2054 arg1Of cells,1
R1732 T2057 T2055 arg1Of cells,×
R1733 T2057 T2056 arg1Of cells,103/well
R1734 T2057 T2058 arg1Of cells,were
R1735 T2057 T2059 arg2Of cells,seeded
R1736 T2057 T2065 arg2Of cells,cultivated
R1737 T2059 T2060 arg1Of seeded,to
R1738 T2059 T2064 arg1Of seeded,and
R1739 T2063 T2060 arg2Of plates,to
R1740 T2063 T2061 arg1Of plates,96-well
R1741 T2063 T2062 arg1Of plates,culture
R1742 T2064 T2058 arg2Of and,were
R1743 T2065 T2064 arg2Of cultivated,and
R1744 T2065 T2066 arg1Of cultivated,for
R1745 T2068 T2066 arg2Of h,for
R1746 T2068 T2067 arg1Of h,24
R1747 T2068 T2070 arg1Of h,adhere
R1748 T2070 T2066 arg3Of adhere,for
R1749 T2070 T2069 arg1Of adhere,to
R1750 T2073 T2072 arg1Of cell,the
R1751 T2073 T2074 arg1Of cell,was
R1752 T2073 T2075 arg2Of cell,scrated
R1753 T2075 T2071 arg1Of scrated,Then
R1754 T2075 T2074 arg2Of scrated,was
R1755 T2075 T2076 arg1Of scrated,as
R1756 T2077 T2076 arg2Of mentioned,as
R1757 T2077 T2078 arg1Of mentioned,above
R1758 T2081 T2079 arg1Of death,Astrocyte
R1759 T2081 T2080 arg1Of death,cell
R1760 T2081 T2082 arg1Of death,was
R1761 T2081 T2083 arg2Of death,assessed
R1762 T2083 T2082 arg2Of assessed,was
R1763 T2083 T2091 arg1Of assessed,(
R1764 T2083 T2101 arg1Of assessed,according
R1765 T2087 T2083 arg1Of kit-8,assessed
R1766 T2087 T2084 arg2Of kit-8,by
R1767 T2087 T2085 arg1Of kit-8,cell
R1768 T2087 T2086 arg1Of kit-8,counting
R1769 T2087 T2088 arg1Of kit-8,(
R1770 T2089 T2088 arg2Of CCK-8,(
R1771 T2090 T2088 arg3Of ),(
R1772 T2092 T2091 arg2Of Dojindo,(
R1773 T2092 T2093 arg1Of Dojindo,","
R1774 T2094 T2093 arg2Of Japan,","
R1775 T2095 T2091 arg3Of ),(
R1776 T2098 T2096 arg1Of h,assay
R1777 T2098 T2097 arg1Of h,24
R1778 T2098 T2099 arg1Of h,after
R1779 T2100 T2099 arg2Of scratch,after
R1780 T2101 T2098 arg1Of according,h
R1781 T2102 T2101 arg2Of to,according
R1782 T2104 T2103 arg1Of manufacturer,the
R1783 T2104 T2105 arg2Of manufacturer,'s
R1784 T2106 T2102 arg2Of protocol,to
R1785 T2106 T2105 arg1Of protocol,'s
R1786 T2107 T2108 arg1Of Briefly,","
R1787 T2107 T2112 arg1Of Briefly,was
R1788 T2107 T2113 arg2Of Briefly,added
R1789 T2107 T2118 arg2Of Briefly,incubated
R1790 T2111 T2108 arg2Of CCK-8,","
R1791 T2111 T2109 arg1Of CCK-8,10
R1792 T2111 T2110 arg1Of CCK-8,μL
R1793 T2113 T2114 arg1Of added,into
R1794 T2113 T2117 arg1Of added,and
R1795 T2116 T2114 arg2Of well,into
R1796 T2116 T2115 arg1Of well,every
R1797 T2117 T2112 arg2Of and,was
R1798 T2118 T2117 arg2Of incubated,and
R1799 T2118 T2119 arg1Of incubated,for
R1800 T2121 T2119 arg2Of h,for
R1801 T2121 T2120 arg1Of h,1
R1802 T2124 T2123 arg1Of value,OD
R1803 T2124 T2125 arg1Of value,was
R1804 T2124 T2126 arg2Of value,read
R1805 T2124 T2130 arg1Of value,using
R1806 T2126 T2122 arg1Of read,Then
R1807 T2126 T2125 arg2Of read,was
R1808 T2126 T2127 arg1Of read,at
R1809 T2126 T2130 modOf read,using
R1810 T2129 T2127 arg2Of nm,at
R1811 T2129 T2128 arg1Of nm,450
R1812 T2130 T2136 arg1Of using,(
R1813 T2135 T2130 arg2Of reader,using
R1814 T2135 T2131 arg1Of reader,a
R1815 T2135 T2132 arg1Of reader,Bio-Rad
R1816 T2135 T2133 arg1Of reader,ELISA
R1817 T2135 T2134 arg1Of reader,microplate
R1818 T2138 T2136 arg2Of Laboratories,(
R1819 T2138 T2137 arg1Of Laboratories,Bio-Rad
R1820 T2138 T2139 arg1Of Laboratories,","
R1821 T2140 T2139 arg2Of CA,","
R1822 T2140 T2141 arg1Of CA,","
R1823 T2142 T2141 arg2Of USA,","
R1824 T2143 T2136 arg3Of ),(
R1825 T2145 T2144 arg1Of measurements,All
R1826 T2145 T2146 arg1Of measurements,were
R1827 T2145 T2147 arg2Of measurements,performed
R1828 T2147 T2146 arg2Of performed,were
R1829 T2147 T2148 arg1Of performed,in
R1830 T2149 T2148 arg2Of sextuplicate,in
R1831 T2150 T2151 arg1Of Results,were
R1832 T2150 T2152 arg2Of Results,expressed
R1833 T2152 T2151 arg2Of expressed,were
R1834 T2152 T2153 arg1Of expressed,as
R1835 T2152 T2158 arg1Of expressed,at
R1836 T2154 T2153 arg2Of mean,as
R1837 T2154 T2155 arg1Of mean,of
R1838 T2157 T2155 arg2Of value,of
R1839 T2157 T2156 arg1Of value,OD
R1840 T2159 T2160 arg1Of 450,nm
R1841 T2162 T2158 arg2Of SD,at
R1842 T2162 T2159 arg1Of SD,450
R1843 T2162 T2161 arg1Of SD,±
R1968 T2327 T2324 arg1Of Assay,Electrophoretic
R1969 T2327 T2325 arg1Of Assay,Mobility
R1970 T2327 T2326 arg1Of Assay,Shift
R1971 T2327 T2328 arg1Of Assay,(
R1972 T2329 T2328 arg2Of EMSA,(
R1973 T2330 T2328 arg3Of ),(
R1974 T2331 T2332 arg1Of Monolayers,of
R1975 T2331 T2334 arg1Of Monolayers,were
R1976 T2331 T2335 arg2Of Monolayers,washed
R1977 T2331 T2339 arg2Of Monolayers,harvested
R1978 T2333 T2332 arg2Of astrocytes,of
R1979 T2335 T2336 arg1Of washed,with
R1980 T2335 T2338 arg1Of washed,and
R1981 T2337 T2336 arg2Of PBS,with
R1982 T2338 T2334 arg2Of and,were
R1983 T2339 T2338 arg2Of harvested,and
R1984 T2339 T2340 arg1Of harvested,by
R1985 T2341 T2340 arg2Of scraping,by
R1986 T2341 T2342 arg1Of scraping,into
R1987 T2344 T2342 arg2Of PBS,into
R1988 T2344 T2343 arg1Of PBS,cold
R1989 T2347 T2345 arg1Of pellet,The
R1990 T2347 T2346 arg1Of pellet,cell
R1991 T2347 T2348 arg2Of pellet,obtained
R1992 T2347 T2351 arg1Of pellet,was
R1993 T2347 T2352 arg2Of pellet,resuspended
R1994 T2350 T2348 arg1Of centrifugation,obtained
R1995 T2350 T2349 arg2Of centrifugation,by
R1996 T2352 T2351 arg2Of resuspended,was
R1997 T2352 T2353 arg1Of resuspended,in
R1998 T2354 T2355 arg1Of buffer,containing
R1999 T2354 T2367 arg1Of buffer,","
R2000 T2356 T2357 arg1Of 10,mM
R2001 T2358 T2356 arg1Of HEPES,10
R2002 T2358 T2359 arg1Of HEPES,(
R2003 T2358 T2363 arg1Of HEPES,","
R2004 T2360 T2359 arg2Of pH,(
R2005 T2360 T2361 arg1Of pH,7.9
R2006 T2362 T2359 arg3Of ),(
R2007 T2363 T2355 arg2Of ",",containing
R2008 T2364 T2365 arg1Of 10,mM
R2009 T2366 T2363 arg2Of KCl,","
R2010 T2366 T2364 arg1Of KCl,10
R2011 T2367 T2353 arg2Of ",",in
R2012 T2368 T2369 arg1Of 0.1,mM
R2013 T2370 T2368 arg1Of EDTA,0.1
R2014 T2370 T2371 arg1Of EDTA,","
R2015 T2371 T2375 arg1Of ",",","
R2016 T2372 T2373 arg1Of 0.1,mM
R2017 T2374 T2371 arg2Of EGTA,","
R2018 T2374 T2372 arg1Of EGTA,0.1
R2019 T2375 T2380 arg1Of ",",and
R2020 T2376 T2377 arg1Of 1.0,mM
R2021 T2378 T2375 arg2Of DTT,","
R2022 T2378 T2376 arg1Of DTT,1.0
R2023 T2380 T2367 arg2Of and,","
R2024 T2380 T2379 arg1Of and,","
R2025 T2381 T2382 arg1Of 0.5,mM
R2026 T2384 T2380 arg2Of fluoride,and
R2027 T2384 T2381 arg1Of fluoride,0.5
R2028 T2384 T2383 arg1Of fluoride,phenylmethylsulfonyl
R2029 T2386 T2385 arg1Of 10,Then
R2030 T2386 T2387 arg1Of 10,%
R2031 T2389 T2386 arg1Of P-40,10
R2032 T2389 T2388 arg1Of P-40,Nonidet
R2033 T2389 T2390 arg1Of P-40,was
R2034 T2389 T2391 arg2Of P-40,added
R2035 T2389 T2393 arg2Of P-40,vortexed
R2036 T2391 T2392 arg1Of added,and
R2037 T2392 T2390 arg2Of and,was
R2038 T2392 T2396 arg1Of and,and
R2039 T2393 T2392 arg2Of vortexed,and
R2040 T2394 T2393 arg3Of briefly,vortexed
R2041 T2396 T2395 arg1Of and,","
R2042 T2398 T2397 arg1Of nuclei,the
R2043 T2398 T2399 arg1Of nuclei,were
R2044 T2398 T2400 arg2Of nuclei,pelleted
R2045 T2400 T2396 arg2Of pelleted,and
R2046 T2400 T2399 arg2Of pelleted,were
R2047 T2402 T2400 arg1Of centrifugation,pelleted
R2048 T2402 T2401 arg2Of centrifugation,by
R2049 T2405 T2403 arg1Of proteins,The
R2050 T2405 T2404 arg1Of proteins,nuclear
R2051 T2405 T2406 arg1Of proteins,were
R2052 T2405 T2407 arg2Of proteins,extracted
R2053 T2407 T2406 arg2Of extracted,were
R2054 T2407 T2408 arg1Of extracted,with
R2055 T2409 T2410 arg1Of buffer,containing
R2056 T2409 T2422 arg1Of buffer,","
R2057 T2411 T2412 arg1Of 20,mM
R2058 T2413 T2411 arg1Of HEPES,20
R2059 T2413 T2414 arg1Of HEPES,(
R2060 T2413 T2418 arg1Of HEPES,","
R2061 T2415 T2414 arg2Of pH,(
R2062 T2415 T2416 arg1Of pH,7.9
R2063 T2417 T2414 arg3Of ),(
R2064 T2418 T2410 arg2Of ",",containing
R2065 T2419 T2420 arg1Of 0.4,mM
R2066 T2421 T2418 arg2Of NaCl,","
R2067 T2421 T2419 arg1Of NaCl,0.4
R2068 T2422 T2408 arg2Of ",",with
R2069 T2423 T2424 arg1Of 1.0,mM
R2070 T2425 T2423 arg1Of EDTA,1.0
R2071 T2425 T2426 arg1Of EDTA,","
R2072 T2426 T2430 arg1Of ",",","
R2073 T2427 T2428 arg1Of 1.0,mM
R2074 T2429 T2426 arg2Of EGTA,","
R2075 T2429 T2427 arg1Of EGTA,1.0
R2076 T2430 T2435 arg1Of ",",and
R2077 T2431 T2432 arg1Of 1.0,mM
R2078 T2433 T2430 arg2Of DTT,","
R2079 T2433 T2431 arg1Of DTT,1.0
R2080 T2435 T2422 arg2Of and,","
R2081 T2435 T2434 arg1Of and,","
R2082 T2436 T2437 arg1Of 1.0,mM
R2083 T2439 T2435 arg2Of fluoride,and
R2084 T2439 T2436 arg1Of fluoride,1.0
R2085 T2439 T2438 arg1Of fluoride,phenylmethylsulfonyl
R2086 T2441 T2440 arg1Of material,Insoluble
R2087 T2441 T2442 arg1Of material,was
R2088 T2441 T2443 arg2Of material,removed
R2089 T2443 T2442 arg2Of removed,was
R2090 T2443 T2446 arg1Of removed,at
R2091 T2443 T2450 arg1Of removed,and
R2092 T2445 T2443 arg1Of centrifugation,removed
R2093 T2445 T2444 arg2Of centrifugation,by
R2094 T2448 T2446 arg2Of rpm,at
R2095 T2448 T2447 arg1Of rpm,14000
R2096 T2450 T2449 arg1Of and,","
R2097 T2452 T2451 arg1Of supernatant,the
R2098 T2452 T2453 arg1Of supernatant,containing
R2099 T2452 T2457 arg1Of supernatant,was
R2100 T2452 T2458 arg2Of supernatant,stored
R2101 T2456 T2453 arg2Of proteins,containing
R2102 T2456 T2454 arg1Of proteins,the
R2103 T2456 T2455 arg1Of proteins,nuclear
R2104 T2458 T2450 arg2Of stored,and
R2105 T2458 T2457 arg2Of stored,was
R2106 T2458 T2459 arg1Of stored,at
R2107 T2458 T2461 arg1Of stored,until
R2108 T2460 T2459 arg2Of –80°C,at
R2109 T2462 T2461 arg2Of use,until
R2110 T2464 T2463 arg1Of concentration,Protein
R2111 T2464 T2465 arg1Of concentration,was
R2112 T2464 T2466 arg2Of concentration,determined
R2113 T2466 T2465 arg2Of determined,was
R2114 T2467 T2466 arg3Of using,determined
R2115 T2467 T2473 arg1Of using,with
R2116 T2472 T2467 arg2Of kit,using
R2117 T2472 T2468 arg1Of kit,a
R2118 T2472 T2469 arg1Of kit,bicinchoninic
R2119 T2472 T2470 arg1Of kit,acid
R2120 T2472 T2471 arg1Of kit,assay
R2121 T2476 T2473 arg2Of albumin,with
R2122 T2476 T2474 arg1Of albumin,bovine
R2123 T2476 T2475 arg1Of albumin,serum
R2124 T2476 T2477 arg1Of albumin,as
R2125 T2482 T2481 arg1Of Biochemicals,Pierce
R2126 T2482 T2483 arg1Of Biochemicals,","
R2127 T2483 T2485 arg1Of ",",","
R2128 T2484 T2483 arg2Of Rockford,","
R2129 T2485 T2477 arg2Of ",",as
R2130 T2485 T2478 arg1Of ",",the
R2131 T2485 T2479 arg1Of ",",standard
R2132 T2485 T2480 arg2Of ",",(
R2133 T2485 T2487 arg1Of ",",","
R2134 T2485 T2488 arg1Of ",",USA
R2135 T2486 T2485 arg2Of IL,","
R2136 T2489 T2480 arg3Of ),(
R2137 T2490 T2491 arg1Of EMSA,was
R2138 T2490 T2492 arg2Of EMSA,performed
R2139 T2492 T2491 arg2Of performed,was
R2140 T2493 T2492 arg3Of using,performed
R2141 T2497 T2493 arg2Of system,using
R2142 T2497 T2494 arg1Of system,gel
R2143 T2497 T2495 arg1Of system,shift
R2144 T2497 T2496 arg1Of system,assay
R2145 T2497 T2498 arg1Of system,(
R2146 T2499 T2500 arg1Of Promega,","
R2147 T2500 T2502 arg1Of ",",","
R2148 T2501 T2500 arg2Of Madison,","
R2149 T2502 T2498 arg2Of ",",(
R2150 T2502 T2504 arg1Of ",",","
R2151 T2502 T2505 arg1Of ",",USA
R2152 T2503 T2502 arg2Of WI,","
R2153 T2506 T2498 arg3Of ),(
R2154 T2509 T2507 arg1Of probe,Consensus
R2155 T2509 T2508 arg1Of probe,oligonucleotide
R2156 T2509 T2510 arg1Of probe,(
R2157 T2509 T2514 arg1Of probe,was
R2158 T2509 T2515 arg2Of probe,end-labeled
R2159 T2509 T2519 arg1Of probe,using
R2160 T2511 T2510 arg2Of 5′-AGTTGAGGGGACTTTCCCAGGG-3,(
R2161 T2511 T2512 arg1Of 5′-AGTTGAGGGGACTTTCCCAGGG-3,′
R2162 T2513 T2510 arg3Of ),(
R2163 T2515 T2514 arg2Of end-labeled,was
R2164 T2515 T2519 modOf end-labeled,using
R2165 T2518 T2515 arg1Of kinase,end-labeled
R2166 T2518 T2516 arg2Of kinase,by
R2167 T2518 T2517 arg1Of kinase,T4-polynucleotide
R2168 T2521 T2520 arg2Of γ-32P,[
R2169 T2522 T2520 arg3Of ],[
R2170 T2523 T2519 arg2Of -ATP,using
R2171 T2523 T2520 arg1Of -ATP,[
R2172 T2523 T2524 arg1Of -ATP,(
R2173 T2526 T2524 arg2Of Biotech.,(
R2174 T2526 T2525 arg1Of Biotech.,Free
R2175 T2526 T2527 arg1Of Biotech.,","
R2176 T2528 T2527 arg2Of Beijing,","
R2177 T2528 T2529 arg1Of Beijing,","
R2178 T2530 T2529 arg2Of China,","
R2179 T2531 T2524 arg3Of ),(
R2180 T2533 T2532 arg1Of protein,Nuclear
R2181 T2533 T2534 arg1Of protein,(
R2182 T2533 T2538 arg1Of protein,was
R2183 T2533 T2539 arg2Of protein,preincubated
R2184 T2536 T2534 arg2Of μg,(
R2185 T2536 T2535 arg1Of μg,20
R2186 T2537 T2534 arg3Of ),(
R2187 T2539 T2538 arg2Of preincubated,was
R2188 T2539 T2540 arg1Of preincubated,in
R2189 T2544 T2540 arg2Of buffer,in
R2190 T2544 T2541 arg1Of buffer,20
R2191 T2544 T2542 arg1Of buffer,μL
R2192 T2544 T2543 arg1Of buffer,binding
R2193 T2544 T2545 arg1Of buffer,containing
R2194 T2546 T2547 arg1Of 10,mM
R2195 T2548 T2546 arg1Of Tris-HCl,10
R2196 T2548 T2549 arg1Of Tris-HCl,(
R2197 T2548 T2553 arg1Of Tris-HCl,","
R2198 T2550 T2549 arg2Of PH,(
R2199 T2550 T2551 arg1Of PH,7.5
R2200 T2552 T2549 arg3Of ),(
R2201 T2553 T2557 arg1Of ",",","
R2202 T2554 T2555 arg1Of 1,mM
R2203 T2556 T2553 arg2Of MgCl2,","
R2204 T2556 T2554 arg1Of MgCl2,1
R2205 T2557 T2561 arg1Of ",",","
R2206 T2558 T2559 arg1Of 0.5,mM
R2207 T2560 T2557 arg2Of NaCl,","
R2208 T2560 T2558 arg1Of NaCl,0.5
R2209 T2561 T2545 arg2Of ",",containing
R2210 T2562 T2563 arg1Of 4,%
R2211 T2564 T2562 arg1Of glycerol,4
R2212 T2564 T2565 arg1Of glycerol,","
R2213 T2565 T2569 arg1Of ",",","
R2214 T2566 T2567 arg1Of 0.5,mM
R2215 T2568 T2565 arg2Of EDTA,","
R2216 T2568 T2566 arg1Of EDTA,0.5
R2217 T2569 T2574 arg1Of ",",and
R2218 T2570 T2571 arg1Of 0.5,mM
R2219 T2572 T2569 arg2Of DTT,","
R2220 T2572 T2570 arg1Of DTT,0.5
R2221 T2574 T2561 arg2Of and,","
R2222 T2574 T2573 arg1Of and,","
R2223 T2578 T2574 arg2Of dI-dC,and
R2224 T2578 T2575 arg1Of dI-dC,2
R2225 T2578 T2576 arg1Of dI-dC,μg
R2226 T2578 T2577 arg1Of dI-dC,poly
R2227 T2578 T2579 arg1Of dI-dC,for
R2228 T2581 T2579 arg2Of minutes,for
R2229 T2581 T2580 arg1Of minutes,20
R2230 T2581 T2582 arg1Of minutes,on
R2231 T2583 T2582 arg2Of ice,on
R2232 T2585 T2584 arg2Of addition,After
R2233 T2585 T2586 arg1Of addition,of
R2234 T2592 T2586 arg2Of probe,of
R2235 T2592 T2587 arg1Of probe,the
R2236 T2592 T2588 arg1Of probe,1
R2237 T2592 T2589 arg1Of probe,μL
R2238 T2592 T2590 arg1Of probe,32P-labled
R2239 T2592 T2591 arg1Of probe,oligonucleotide
R2240 T2595 T2594 arg1Of incubation,the
R2241 T2595 T2596 arg1Of incubation,was
R2242 T2595 T2597 arg2Of incubation,continued
R2243 T2597 T2584 arg1Of continued,After
R2244 T2597 T2593 arg1Of continued,","
R2245 T2597 T2596 arg2Of continued,was
R2246 T2597 T2598 arg1Of continued,for
R2247 T2600 T2598 arg2Of minutes,for
R2248 T2600 T2599 arg1Of minutes,20
R2249 T2600 T2601 arg1Of minutes,on
R2250 T2602 T2601 arg2Of ice,on
R2251 T2605 T2603 arg1Of complexes,The
R2252 T2605 T2604 arg1Of complexes,DNA-protein
R2253 T2605 T2606 arg1Of complexes,were
R2254 T2605 T2607 arg2Of complexes,separated
R2255 T2607 T2606 arg2Of separated,were
R2256 T2607 T2610 arg1Of separated,on
R2257 T2607 T2616 arg1Of separated,in
R2258 T2609 T2607 arg1Of electrophoresis,separated
R2259 T2609 T2608 arg2Of electrophoresis,by
R2260 T2611 T2612 arg1Of 4,%
R2261 T2615 T2610 arg2Of gel,on
R2262 T2615 T2611 arg1Of gel,4
R2263 T2615 T2613 arg1Of gel,nondenaturing
R2264 T2615 T2614 arg1Of gel,polyacrylamide
R2265 T2620 T2616 arg2Of buffer,in
R2266 T2620 T2617 arg1Of buffer,0.5
R2267 T2620 T2618 arg1Of buffer,×
R2268 T2620 T2619 arg1Of buffer,TBE
R2269 T2620 T2621 arg1Of buffer,(
R2270 T2620 T2624 arg1Of buffer,at
R2271 T2622 T2621 arg2Of tris-borate-EDTA,(
R2272 T2623 T2621 arg3Of ),(
R2273 T2626 T2624 arg2Of V,at
R2274 T2626 T2625 arg1Of V,390
R2275 T2626 T2627 arg1Of V,for
R2276 T2629 T2627 arg2Of hour,for
R2277 T2629 T2628 arg1Of hour,1
R2278 T2629 T2630 arg1Of hour,at
R2279 T2631 T2630 arg2Of 4°C,at
R2280 T2633 T2632 arg2Of electrophoresis,After
R2281 T2636 T2635 arg1Of gel,the
R2282 T2636 T2637 arg1Of gel,was
R2283 T2636 T2638 arg2Of gel,dried
R2284 T2636 T2640 arg2Of gel,exposed
R2285 T2638 T2639 arg1Of dried,and
R2286 T2639 T2632 arg1Of and,After
R2287 T2639 T2634 arg1Of and,","
R2288 T2639 T2637 arg2Of and,was
R2289 T2640 T2639 arg2Of exposed,and
R2290 T2640 T2641 arg1Of exposed,to
R2291 T2643 T2641 arg2Of film,to
R2292 T2643 T2642 arg1Of film,X-ray
R2293 T2643 T2644 arg1Of film,(
R2294 T2646 T2644 arg2Of Hyperfilm,(
R2295 T2646 T2645 arg1Of Hyperfilm,Fuji
R2296 T2646 T2647 arg1Of Hyperfilm,","
R2297 T2648 T2647 arg2Of Tokyo,","
R2298 T2648 T2649 arg1Of Tokyo,","
R2299 T2650 T2649 arg2Of Japan,","
R2300 T2651 T2644 arg3Of ),(
R2301 T2652 T2653 arg1Of Levels,of
R2302 T2652 T2658 arg1Of Levels,were
R2303 T2652 T2659 arg2Of Levels,quantified
R2304 T2657 T2653 arg2Of activity,of
R2305 T2657 T2654 arg1Of activity,NF-κB
R2306 T2657 T2655 arg1Of activity,DNA
R2307 T2657 T2656 arg1Of activity,binding
R2308 T2659 T2658 arg2Of quantified,were
R2309 T2662 T2659 arg1Of ImageJ,quantified
R2310 T2662 T2660 arg2Of ImageJ,by
R2311 T2662 T2661 arg1Of ImageJ,software
R2677 T3118 T3117 arg1Of RNA,Total
R2678 T3118 T3119 arg1Of RNA,was
R2679 T3118 T3120 arg2Of RNA,isolated
R2680 T3120 T3119 arg2Of isolated,was
R2681 T3120 T3121 arg1Of isolated,with
R2682 T3120 T3131 arg1Of isolated,and
R2683 T3122 T3121 arg2Of Trizol,with
R2684 T3122 T3123 arg1Of Trizol,(
R2685 T3124 T3123 arg2Of Invitrogen,(
R2686 T3124 T3125 arg1Of Invitrogen,","
R2687 T3124 T3127 arg1Of Invitrogen,","
R2688 T3124 T3128 arg1Of Invitrogen,USA
R2689 T3126 T3125 arg2Of CA,","
R2690 T3129 T3123 arg3Of ),(
R2691 T3131 T3130 arg1Of and,","
R2692 T3131 T3145 arg1Of and,(
R2693 T3131 T3152 arg1Of and,according
R2694 T3133 T3132 arg1Of cDNA,single-stranded
R2695 T3133 T3134 arg1Of cDNA,was
R2696 T3133 T3135 arg2Of cDNA,synthesized
R2697 T3135 T3131 arg2Of synthesized,and
R2698 T3135 T3134 arg2Of synthesized,was
R2699 T3135 T3136 arg1Of synthesized,from
R2700 T3138 T3136 arg2Of μg,from
R2701 T3138 T3137 arg1Of μg,2
R2702 T3138 T3139 arg1Of μg,of
R2703 T3141 T3139 arg2Of RNA,of
R2704 T3141 T3140 arg1Of RNA,total
R2705 T3141 T3142 arg1Of RNA,with
R2706 T3144 T3142 arg2Of RT-Kit,with
R2707 T3144 T3143 arg1Of RT-Kit,BU-Script
R2708 T3146 T3147 arg1Of Biunique,","
R2709 T3147 T3145 arg2Of ",",(
R2710 T3147 T3149 arg1Of ",",","
R2711 T3148 T3147 arg2Of Jiangsu,","
R2712 T3150 T3149 arg2Of China,","
R2713 T3151 T3145 arg3Of ),(
R2714 T3153 T3152 arg2Of to,according
R2715 T3155 T3154 arg1Of manufacturer,the
R2716 T3155 T3156 arg2Of manufacturer,'s
R2717 T3157 T3153 arg2Of protocol,to
R2718 T3157 T3156 arg1Of protocol,'s
R2719 T3159 T3158 arg1Of cDNA,The
R2720 T3159 T3160 arg1Of cDNA,was
R2721 T3159 T3161 arg2Of cDNA,stored
R2722 T3161 T3160 arg2Of stored,was
R2723 T3161 T3162 arg1Of stored,in
R2724 T3163 T3162 arg2Of −20°C,in
R2725 T3165 T3164 arg1Of transcription,Reverse
R2726 T3165 T3166 arg1Of transcription,was
R2727 T3165 T3167 arg2Of transcription,conducted
R2728 T3167 T3166 arg2Of conducted,was
R2729 T3167 T3168 arg1Of conducted,with
R2730 T3167 T3180 arg1Of conducted,according
R2731 T3172 T3168 arg2Of Mix,with
R2732 T3172 T3169 arg1Of Mix,GoTaq
R2733 T3172 T3170 arg1Of Mix,Green
R2734 T3172 T3171 arg1Of Mix,Master
R2735 T3172 T3173 arg1Of Mix,(
R2736 T3174 T3175 arg1Of Promega,","
R2737 T3175 T3173 arg2Of ",",(
R2738 T3175 T3177 arg1Of ",",","
R2739 T3175 T3178 arg1Of ",",USA
R2740 T3176 T3175 arg2Of WI,","
R2741 T3179 T3173 arg3Of ),(
R2742 T3181 T3180 arg2Of to,according
R2743 T3183 T3182 arg1Of manufacturer,the
R2744 T3183 T3184 arg2Of manufacturer,'s
R2745 T3185 T3181 arg2Of protocol,to
R2746 T3185 T3184 arg1Of protocol,'s
R2747 T3187 T3186 arg1Of 1,Table
R2748 T3187 T3188 arg1Of 1,shows
R2749 T3190 T3191 arg1Of primers,and
R2750 T3191 T3188 arg2Of and,shows
R2751 T3191 T3189 arg1Of and,the
R2752 T3193 T3191 arg2Of parameters,and
R2753 T3193 T3192 arg1Of parameters,PCR
R2754 T3195 T3194 arg1Of products,PCR
R2755 T3195 T3196 arg1Of products,were
R2756 T3195 T3197 arg2Of products,detected
R2757 T3197 T3196 arg2Of detected,were
R2758 T3201 T3197 arg1Of electrophoresis,detected
R2759 T3201 T3198 arg2Of electrophoresis,by
R2760 T3201 T3199 arg1Of electrophoresis,agarose
R2761 T3201 T3200 arg1Of electrophoresis,gel
R2762 T3203 T3202 arg1Of intensity,The
R2763 T3203 T3204 arg1Of intensity,of
R2764 T3203 T3207 arg1Of intensity,was
R2765 T3203 T3208 arg2Of intensity,analyzed
R2766 T3206 T3204 arg2Of bands,of
R2767 T3206 T3205 arg1Of bands,the
R2768 T3208 T3207 arg2Of analyzed,was
R2769 T3211 T3208 arg1Of program,analyzed
R2770 T3211 T3209 arg2Of program,by
R2771 T3211 T3210 arg1Of program,ImageJ
R2772 T3213 T3212 arg1Of level,The
R2773 T3213 T3214 arg1Of level,of
R2774 T3213 T3216 arg1Of level,was
R2775 T3213 T3217 arg2Of level,used
R2776 T3215 T3214 arg2Of β-actin,of
R2777 T3217 T3216 arg2Of used,was
R2778 T3217 T3218 arg1Of used,as
R2779 T3221 T3218 arg2Of standard,as
R2780 T3221 T3219 arg1Of standard,an
R2781 T3221 T3220 arg1Of standard,internal
R2899 T3391 T3390 arg1Of Blot,Western
R2900 T3393 T3392 arg1Of obtain,To
R2901 T3396 T3393 arg2Of lysates,obtain
R2902 T3396 T3394 arg1Of lysates,total
R2903 T3396 T3395 arg1Of lysates,protein
R2904 T3398 T3393 arg1Of cells,obtain
R2905 T3398 T3399 arg1Of cells,were
R2906 T3398 T3400 arg2Of cells,homogenized
R2907 T3398 T3452 arg2Of cells,centrifuged
R2908 T3400 T3401 arg1Of homogenized,in
R2909 T3400 T3451 arg1Of homogenized,and
R2910 T3403 T3401 arg2Of buffer,in
R2911 T3403 T3402 arg1Of buffer,RIPA
R2912 T3403 T3404 arg1Of buffer,(
R2913 T3405 T3406 arg1Of 1,%
R2914 T3407 T3405 arg1Of NP40,1
R2915 T3407 T3408 arg1Of NP40,","
R2916 T3408 T3413 arg1Of ",",","
R2917 T3409 T3410 arg1Of 0.5,%
R2918 T3412 T3408 arg2Of deoxycholate,","
R2919 T3412 T3409 arg1Of deoxycholate,0.5
R2920 T3412 T3411 arg1Of deoxycholate,sodium
R2921 T3413 T3417 arg1Of ",",","
R2922 T3414 T3415 arg1Of 0.1,%
R2923 T3416 T3413 arg2Of SDS,","
R2924 T3416 T3414 arg1Of SDS,0.1
R2925 T3417 T3421 arg1Of ",",","
R2926 T3418 T3419 arg1Of 1,mM
R2927 T3420 T3417 arg2Of EDTA,","
R2928 T3420 T3418 arg1Of EDTA,1
R2929 T3421 T3404 arg2Of ",",(
R2930 T3421 T3425 arg1Of ",",","
R2931 T3422 T3423 arg1Of 1,mM
R2932 T3424 T3421 arg2Of EGTA,","
R2933 T3424 T3422 arg1Of EGTA,1
R2934 T3426 T3427 arg1Of 1,mM
R2935 T3428 T3426 arg1Of Na3VO4,1
R2936 T3428 T3429 arg1Of Na3VO4,","
R2937 T3429 T3433 arg1Of ",",","
R2938 T3430 T3431 arg1Of 20,mM
R2939 T3432 T3429 arg2Of NaF,","
R2940 T3432 T3430 arg1Of NaF,20
R2941 T3433 T3437 arg1Of ",",","
R2942 T3434 T3435 arg1Of 0.5,mM
R2943 T3436 T3433 arg2Of DTT,","
R2944 T3436 T3434 arg1Of DTT,0.5
R2945 T3437 T3442 arg1Of ",",and
R2946 T3438 T3439 arg1Of 1,mM
R2947 T3440 T3437 arg2Of PMSF,","
R2948 T3440 T3438 arg1Of PMSF,1
R2949 T3442 T3425 arg2Of and,","
R2950 T3442 T3441 arg1Of and,","
R2951 T3445 T3442 arg2Of cocktail,and
R2952 T3445 T3443 arg1Of cocktail,protease
R2953 T3445 T3444 arg1Of cocktail,inhibitor
R2954 T3445 T3446 arg1Of cocktail,in
R2955 T3448 T3446 arg2Of pH,in
R2956 T3448 T3447 arg1Of pH,PBS
R2957 T3448 T3449 arg1Of pH,7.4
R2958 T3450 T3404 arg3Of ),(
R2959 T3451 T3392 modOf and,To
R2960 T3451 T3397 arg1Of and,","
R2961 T3451 T3399 arg2Of and,were
R2962 T3452 T3451 arg2Of centrifuged,and
R2963 T3452 T3453 arg1Of centrifuged,at
R2964 T3452 T3456 arg1Of centrifuged,for
R2965 T3455 T3453 arg2Of g,at
R2966 T3455 T3454 arg1Of g,"12,000"
R2967 T3458 T3456 arg2Of min,for
R2968 T3458 T3457 arg1Of min,15
R2969 T3458 T3459 arg1Of min,at
R2970 T3460 T3459 arg2Of 4°C,at
R2971 T3462 T3461 arg1Of concentrations,Protein
R2972 T3462 T3463 arg1Of concentrations,were
R2973 T3462 T3464 arg2Of concentrations,estimated
R2974 T3464 T3463 arg2Of estimated,were
R2975 T3470 T3464 arg1Of Reagent,estimated
R2976 T3470 T3465 arg2Of Reagent,by
R2977 T3470 T3466 arg1Of Reagent,Coomassie
R2978 T3470 T3467 arg1Of Reagent,Plus
R2979 T3470 T3468 arg1Of Reagent,Protein
R2980 T3470 T3469 arg1Of Reagent,Assay
R2981 T3470 T3471 arg1Of Reagent,(
R2982 T3472 T3473 arg1Of Pierce,","
R2983 T3473 T3471 arg2Of ",",(
R2984 T3473 T3475 arg1Of ",",","
R2985 T3474 T3473 arg2Of IL,","
R2986 T3476 T3475 arg2Of USA,","
R2987 T3477 T3471 arg3Of ),(
R2988 T3479 T3478 arg1Of micrograms,Fifty
R2989 T3479 T3480 arg1Of micrograms,of
R2990 T3479 T3486 arg1Of micrograms,were
R2991 T3479 T3487 arg1Of micrograms,heat-denatured
R2992 T3479 T3506 arg1Of micrograms,electroblotted
R2993 T3485 T3480 arg2Of extracts,of
R2994 T3485 T3481 arg1Of extracts,the
R2995 T3485 T3482 arg1Of extracts,resulting
R2996 T3485 T3483 arg1Of extracts,cytosolic
R2997 T3485 T3484 arg1Of extracts,protein
R2998 T3486 T3488 arg1Of were,in
R2999 T3486 T3505 arg1Of were,and
R3000 T3487 T3486 arg2Of heat-denatured,were
R3001 T3492 T3488 arg2Of buffer,in
R3002 T3492 T3489 arg1Of buffer,Laemmli
R3003 T3492 T3490 arg1Of buffer,sample
R3004 T3492 T3491 arg1Of buffer,loading
R3005 T3492 T3493 arg1Of buffer,","
R3006 T3492 T3494 arg2Of buffer,separated
R3007 T3496 T3497 arg1Of 10,%
R3008 T3503 T3494 arg1Of electrophoresis,separated
R3009 T3503 T3495 arg2Of electrophoresis,by
R3010 T3503 T3496 arg1Of electrophoresis,10
R3011 T3503 T3498 arg1Of electrophoresis,sodium
R3012 T3503 T3499 arg1Of electrophoresis,dodecyl
R3013 T3503 T3500 arg1Of electrophoresis,sulfate
R3014 T3503 T3501 arg1Of electrophoresis,polyacrylamide
R3015 T3503 T3502 arg1Of electrophoresis,gel
R3016 T3505 T3504 arg1Of and,","
R3017 T3506 T3505 arg2Of electroblotted,and
R3018 T3506 T3507 arg1Of electroblotted,onto
R3019 T3510 T3507 arg2Of membrane,onto
R3020 T3510 T3508 arg1Of membrane,a
R3021 T3510 T3509 arg1Of membrane,nitrocellulose
R3022 T3512 T3511 arg2Of immunoblotting,For
R3023 T3514 T3515 arg1Of membranes,were
R3024 T3514 T3516 arg2Of membranes,blocked
R3025 T3516 T3511 arg1Of blocked,For
R3026 T3516 T3513 arg1Of blocked,","
R3027 T3516 T3515 arg2Of blocked,were
R3028 T3516 T3517 arg1Of blocked,with
R3029 T3516 T3526 arg1Of blocked,overnight
R3030 T3516 T3527 arg1Of blocked,at
R3031 T3516 T3530 arg1Of blocked,and
R3032 T3518 T3519 arg1Of 5,%
R3033 T3522 T3517 arg2Of milk,with
R3034 T3522 T3518 arg1Of milk,5
R3035 T3522 T3520 arg1Of milk,nonfat
R3036 T3522 T3521 arg1Of milk,dry
R3037 T3522 T3523 arg1Of milk,in
R3038 T3525 T3523 arg2Of buffer,in
R3039 T3525 T3524 arg1Of buffer,saline
R3040 T3528 T3527 arg2Of 4°C,at
R3041 T3530 T3529 arg1Of and,","
R3042 T3530 T3536 arg1Of and,:
R3043 T3530 T3537 arg1Of and,anti-β-actin
R3044 T3530 T3554 arg1Of and,metallopeptidase
R3045 T3533 T3531 arg1Of antibodies,the
R3046 T3533 T3532 arg1Of antibodies,following
R3047 T3533 T3534 arg1Of antibodies,were
R3048 T3533 T3535 arg2Of antibodies,used
R3049 T3535 T3530 arg2Of used,and
R3050 T3535 T3534 arg2Of used,were
R3051 T3537 T3538 arg1Of anti-β-actin,(
R3052 T3537 T3552 arg1Of anti-β-actin,and
R3053 T3539 T3540 arg1Of sc-130657,","
R3054 T3539 T3544 arg1Of sc-130657,","
R3055 T3543 T3540 arg2Of Biotechnology,","
R3056 T3543 T3541 arg1Of Biotechnology,Santa
R3057 T3543 T3542 arg1Of Biotechnology,Cruz
R3058 T3544 T3538 arg2Of ",",(
R3059 T3545 T3546 arg1Of CA,","
R3060 T3546 T3548 arg1Of ",",","
R3061 T3547 T3546 arg2Of USA,","
R3062 T3549 T3548 arg2Of 43,","
R3063 T3550 T3544 arg2Of kDa,","
R3064 T3550 T3545 arg1Of kDa,CA
R3065 T3550 T3547 arg1Of kDa,USA
R3066 T3550 T3549 arg1Of kDa,43
R3067 T3551 T3538 arg3Of ),(
R3068 T3552 T3559 arg1Of and,(
R3069 T3554 T3552 arg2Of metallopeptidase,and
R3070 T3554 T3553 arg1Of metallopeptidase,anti-matrix
R3071 T3554 T3555 arg1Of metallopeptidase,9
R3072 T3554 T3556 arg1Of metallopeptidase,(
R3073 T3557 T3556 arg2Of MMP9,(
R3074 T3558 T3556 arg3Of ),(
R3075 T3560 T3561 arg1Of sc-6841,","
R3076 T3560 T3565 arg1Of sc-6841,","
R3077 T3564 T3561 arg2Of Biotechnology,","
R3078 T3564 T3562 arg1Of Biotechnology,Santa
R3079 T3564 T3563 arg1Of Biotechnology,Cruz
R3080 T3565 T3559 arg2Of ",",(
R3081 T3566 T3567 arg1Of CA,","
R3082 T3567 T3569 arg1Of ",",","
R3083 T3568 T3567 arg2Of USA,","
R3084 T3570 T3569 arg2Of 92,","
R3085 T3571 T3565 arg2Of kDa,","
R3086 T3571 T3566 arg1Of kDa,CA
R3087 T3571 T3568 arg1Of kDa,USA
R3088 T3571 T3570 arg1Of kDa,92
R3089 T3572 T3559 arg3Of ),(
R3090 T3575 T3573 arg1Of antibody,Each
R3091 T3575 T3574 arg1Of antibody,primary
R3092 T3575 T3576 arg1Of antibody,was
R3093 T3575 T3577 arg2Of antibody,diluted
R3094 T3575 T3584 arg1Of antibody,added
R3095 T3577 T3576 arg2Of diluted,was
R3096 T3577 T3578 arg1Of diluted,appropriately
R3097 T3577 T3579 arg1Of diluted,in
R3098 T3577 T3582 arg1Of diluted,and
R3099 T3580 T3579 arg2Of blocking,in
R3100 T3581 T3580 arg2Of buffer,blocking
R3101 T3584 T3582 arg2Of added,and
R3102 T3584 T3583 arg1Of added,then
R3103 T3584 T3585 arg1Of added,to
R3104 T3584 T3588 arg1Of added,for
R3105 T3587 T3585 arg2Of blots,to
R3106 T3587 T3586 arg1Of blots,the
R3107 T3590 T3588 arg2Of h,for
R3108 T3590 T3589 arg1Of h,1
R3109 T3590 T3591 arg1Of h,at
R3110 T3593 T3591 arg2Of temperature,at
R3111 T3593 T3592 arg1Of temperature,room
R3112 T3595 T3594 arg1Of blots,The
R3113 T3595 T3596 arg1Of blots,were
R3114 T3595 T3597 arg2Of blots,washed
R3115 T3595 T3605 arg2Of blots,covered
R3116 T3597 T3599 arg1Of washed,times
R3117 T3597 T3600 arg1Of washed,in
R3118 T3597 T3604 arg1Of washed,and
R3119 T3599 T3598 arg1Of times,three
R3120 T3603 T3600 arg2Of buffer,in
R3121 T3603 T3601 arg1Of buffer,the
R3122 T3603 T3602 arg1Of buffer,washing
R3123 T3604 T3596 arg2Of and,were
R3124 T3605 T3604 arg2Of covered,and
R3125 T3605 T3606 arg1Of covered,with
R3126 T3605 T3612 arg1Of covered,at
R3127 T3611 T3606 arg2Of antibody,with
R3128 T3611 T3607 arg1Of antibody,the
R3129 T3611 T3608 arg1Of antibody,horseradish
R3130 T3611 T3609 arg1Of antibody,peroxidase-linked
R3131 T3611 T3610 arg1Of antibody,secondary
R3132 T3614 T3612 arg2Of 1,at
R3133 T3614 T3613 arg1Of 1,a
R3134 T3614 T3615 arg1Of 1,:
R3135 T3617 T3616 arg1Of dilution,2000
R3136 T3617 T3618 arg1Of dilution,for
R3137 T3620 T3618 arg2Of h,for
R3138 T3620 T3619 arg1Of h,1
R3139 T3621 T3622 arg1Of Blots,were
R3140 T3621 T3623 arg2Of Blots,incubated
R3141 T3621 T3641 arg2Of Blots,exposed
R3142 T3623 T3624 arg1Of incubated,with
R3143 T3623 T3640 arg1Of incubated,and
R3144 T3628 T3627 arg2Of ECL,(
R3145 T3629 T3627 arg3Of ),(
R3146 T3631 T3624 arg2Of system,with
R3147 T3631 T3625 arg2Of system,enhanced
R3148 T3631 T3626 arg1Of system,chemiluminescence
R3149 T3631 T3627 arg1Of system,(
R3150 T3631 T3630 arg1Of system,detection
R3151 T3631 T3632 arg1Of system,(
R3152 T3634 T3632 arg2Of Biosciences,(
R3153 T3634 T3633 arg1Of Biosciences,Amersham
R3154 T3634 T3635 arg1Of Biosciences,","
R3155 T3636 T3635 arg2Of Bucks,","
R3156 T3636 T3637 arg1Of Bucks,","
R3157 T3638 T3637 arg2Of UK,","
R3158 T3639 T3632 arg3Of ),(
R3159 T3640 T3622 arg2Of and,were
R3160 T3641 T3640 arg2Of exposed,and
R3161 T3641 T3642 arg1Of exposed,to
R3162 T3644 T3642 arg2Of film,to
R3163 T3644 T3643 arg1Of film,radiographic
R3164 T3644 T3645 arg1Of film,(
R3165 T3647 T3645 arg2Of Hyperfilm,(
R3166 T3647 T3646 arg1Of Hyperfilm,Fuji
R3167 T3647 T3648 arg1Of Hyperfilm,","
R3168 T3649 T3648 arg2Of Tokyo,","
R3169 T3649 T3650 arg1Of Tokyo,","
R3170 T3651 T3650 arg2Of Japan,","
R3171 T3652 T3645 arg3Of ),(
R3172 T3653 T3654 arg1Of ImageJ,was
R3173 T3653 T3655 arg2Of ImageJ,used
R3174 T3655 T3654 arg2Of used,was
R3175 T3657 T3655 arg3Of analyze,used
R3176 T3657 T3656 arg1Of analyze,to
R3177 T3659 T3657 arg2Of intensity,analyze
R3178 T3659 T3658 arg1Of intensity,the
R3179 T3659 T3660 arg1Of intensity,of
R3180 T3662 T3660 arg2Of blots,of
R3181 T3662 T3661 arg1Of blots,the
R3182 T3664 T3663 arg1Of level,The
R3183 T3664 T3665 arg1Of level,of
R3184 T3664 T3667 arg1Of level,was
R3185 T3664 T3668 arg2Of level,used
R3186 T3666 T3665 arg2Of β-actin,of
R3187 T3668 T3667 arg2Of used,was
R3188 T3668 T3669 arg1Of used,as
R3189 T3671 T3669 arg2Of standard,as
R3190 T3671 T3670 arg1Of standard,internal
R3492 T4084 T4082 arg1Of Assay,Enzyme-Linked
R3493 T4084 T4083 arg1Of Assay,Immunosorbent
R3494 T4084 T4085 arg1Of Assay,(
R3495 T4086 T4085 arg2Of ELISA,(
R3496 T4087 T4085 arg3Of ),(
R3497 T4088 T4089 arg1Of Cells,of
R3498 T4088 T4092 arg1Of Cells,were
R3499 T4088 T4093 arg2Of Cells,homogenized
R3500 T4091 T4089 arg2Of groups,of
R3501 T4091 T4090 arg1Of groups,four
R3502 T4093 T4092 arg2Of homogenized,were
R3503 T4093 T4094 arg1Of homogenized,as
R3504 T4095 T4094 arg2Of mentioned,as
R3505 T4095 T4096 arg1Of mentioned,above
R3506 T4098 T4097 arg1Of supernatant,The
R3507 T4098 T4099 arg1Of supernatant,was
R3508 T4098 T4100 arg2Of supernatant,collected
R3509 T4100 T4099 arg2Of collected,was
R3510 T4100 T4102 arg1Of collected,and
R3511 T4102 T4101 arg1Of and,","
R3512 T4104 T4103 arg1Of protein,total
R3513 T4104 T4105 arg1Of protein,was
R3514 T4104 T4106 arg2Of protein,determined
R3515 T4106 T4102 arg2Of determined,and
R3516 T4106 T4105 arg2Of determined,was
R3517 T4112 T4106 arg1Of Reagent,determined
R3518 T4112 T4107 arg2Of Reagent,by
R3519 T4112 T4108 arg1Of Reagent,Coomassie
R3520 T4112 T4109 arg1Of Reagent,Plus
R3521 T4112 T4110 arg1Of Reagent,Protein
R3522 T4112 T4111 arg1Of Reagent,Assay
R3523 T4112 T4113 arg1Of Reagent,(
R3524 T4114 T4115 arg1Of Pierce,","
R3525 T4115 T4113 arg2Of ",",(
R3526 T4115 T4117 arg1Of ",",","
R3527 T4116 T4115 arg2Of IL,","
R3528 T4118 T4117 arg2Of USA,","
R3529 T4119 T4113 arg3Of ),(
R3530 T4120 T4121 arg1Of Levels,of
R3531 T4120 T4135 arg1Of Levels,and
R3532 T4124 T4122 arg1Of factor-alpha,tumor
R3533 T4124 T4123 arg1Of factor-alpha,necrosis
R3534 T4124 T4125 arg1Of factor-alpha,(
R3535 T4124 T4128 arg1Of factor-alpha,","
R3536 T4126 T4125 arg2Of TNF-α,(
R3537 T4127 T4125 arg3Of ),(
R3538 T4128 T4121 arg2Of ",",of
R3539 T4130 T4128 arg2Of beta,","
R3540 T4130 T4129 arg1Of beta,interleukin-1
R3541 T4130 T4131 arg1Of beta,(
R3542 T4132 T4131 arg2Of IL-1β,(
R3543 T4133 T4131 arg3Of ),(
R3544 T4135 T4134 arg1Of and,","
R3545 T4136 T4135 arg2Of interleukin-6,and
R3546 T4136 T4137 arg1Of interleukin-6,(
R3547 T4136 T4141 modOf interleukin-6,were
R3548 T4138 T4137 arg2Of IL-6,(
R3549 T4139 T4137 arg3Of ),(
R3550 T4140 T4141 arg1Of protein,were
R3551 T4140 T4142 arg2Of protein,quantified
R3552 T4142 T4141 arg2Of quantified,were
R3553 T4143 T4142 arg3Of using,quantified
R3554 T4143 T4149 arg1Of using,according
R3555 T4145 T4143 arg2Of kits,using
R3556 T4145 T4144 arg1Of kits,ELISA
R3557 T4145 T4146 arg1Of kits,specific
R3558 T4146 T4147 arg1Of specific,for
R3559 T4148 T4147 arg2Of mouse,for
R3560 T4150 T4149 arg2Of to,according
R3561 T4152 T4151 arg1Of manufacturer,the
R3562 T4152 T4153 arg2Of manufacturer,'s
R3563 T4154 T4150 arg2Of instructions,to
R3564 T4154 T4153 arg1Of instructions,'s
R3565 T4154 T4155 arg1Of instructions,(
R3566 T4159 T4155 arg2Of CA,(
R3567 T4159 T4156 arg1Of CA,Bender
R3568 T4159 T4157 arg1Of CA,MedSystems
R3569 T4159 T4158 arg1Of CA,Inc.
R3570 T4159 T4160 arg1Of CA,","
R3571 T4159 T4161 arg1Of CA,USA
R3572 T4162 T4155 arg3Of ),(
R3573 T4163 T4165 arg1Of Briefly,prepared
R3574 T4163 T4169 arg1Of Briefly,created
R3575 T4165 T4168 arg1Of prepared,and
R3576 T4167 T4165 arg2Of standard,prepared
R3577 T4167 T4166 arg1Of standard,the
R3578 T4168 T4164 arg1Of and,","
R3579 T4169 T4168 arg2Of created,and
R3580 T4172 T4169 arg2Of dilution,created
R3581 T4172 T4170 arg1Of dilution,the
R3582 T4172 T4171 arg1Of dilution,standard
R3583 T4172 T4173 arg1Of dilution,for
R3584 T4174 T4173 arg2Of building,for
R3585 T4176 T4174 arg2Of curve,building
R3586 T4176 T4175 arg1Of curve,standard
R3587 T4178 T4179 arg1Of samples,and
R3588 T4179 T4177 arg1Of and,Then
R3589 T4179 T4181 arg1Of and,were
R3590 T4179 T4182 arg2Of and,added
R3591 T4180 T4179 arg2Of biotinconjugate,and
R3592 T4182 T4181 arg2Of added,were
R3593 T4182 T4183 modOf added,to
R3594 T4184 T4183 arg1Of microwell,to
R3595 T4185 T4184 arg2Of strips,microwell
R3596 T4187 T4188 arg1Of incubated,for
R3597 T4190 T4188 arg2Of h,for
R3598 T4190 T4189 arg1Of h,2
R3599 T4190 T4191 arg1Of h,at
R3600 T4192 T4186 arg1Of room,After
R3601 T4192 T4187 arg2Of room,incubated
R3602 T4193 T4192 arg1Of temperature,room
R3603 T4193 T4194 arg1Of temperature,","
R3604 T4193 T4198 arg1Of temperature,were
R3605 T4193 T4199 arg2Of temperature,washed
R3606 T4197 T4194 arg2Of strips,","
R3607 T4197 T4195 arg1Of strips,the
R3608 T4197 T4196 arg1Of strips,microwell
R3609 T4199 T4198 arg2Of washed,were
R3610 T4199 T4202 arg1Of washed,with
R3611 T4199 T4206 arg1Of washed,and
R3612 T4201 T4199 arg3Of times,washed
R3613 T4201 T4200 arg1Of times,3
R3614 T4204 T4202 arg2Of buffer,with
R3615 T4204 T4203 arg1Of buffer,wash
R3616 T4206 T4205 arg1Of and,","
R3617 T4207 T4208 arg1Of streptavidin-HRP,were
R3618 T4207 T4209 arg2Of streptavidin-HRP,added
R3619 T4209 T4206 arg2Of added,and
R3620 T4209 T4208 arg2Of added,were
R3621 T4209 T4210 arg1Of added,to
R3622 T4212 T4210 arg2Of wells,to
R3623 T4212 T4211 arg1Of wells,all
R3624 T4214 T4215 arg1Of incubated,for
R3625 T4217 T4215 arg2Of h,for
R3626 T4217 T4216 arg1Of h,1
R3627 T4220 T4213 arg1Of strips,After
R3628 T4220 T4214 arg2Of strips,incubated
R3629 T4220 T4218 arg1Of strips,","
R3630 T4220 T4219 arg1Of strips,microwell
R3631 T4220 T4221 arg1Of strips,were
R3632 T4220 T4222 arg2Of strips,washed
R3633 T4222 T4221 arg2Of washed,were
R3634 T4224 T4222 arg3Of times,washed
R3635 T4224 T4223 arg1Of times,3
R3636 T4224 T4225 arg2Of times,followed
R3637 T4225 T4226 arg1Of followed,by
R3638 T4227 T4226 arg2Of adding,by
R3639 T4229 T4227 arg2Of substrate,adding
R3640 T4229 T4228 arg1Of substrate,TMB
R3641 T4231 T4232 arg1Of incubated,for
R3642 T4234 T4230 arg1Of 10–30,After
R3643 T4234 T4231 arg2Of 10–30,incubated
R3644 T4234 T4233 arg1Of 10–30,about
R3645 T4235 T4234 arg1Of min,10–30
R3646 T4235 T4236 arg1Of min,","
R3647 T4235 T4240 arg1Of min,was
R3648 T4235 T4241 arg2Of min,added
R3649 T4239 T4236 arg2Of solution,","
R3650 T4239 T4237 arg1Of solution,the
R3651 T4239 T4238 arg1Of solution,stop
R3652 T4241 T4240 arg2Of added,was
R3653 T4244 T4242 arg1Of intensity,The
R3654 T4244 T4243 arg1Of intensity,colour
R3655 T4244 T4245 arg1Of intensity,was
R3656 T4244 T4246 arg2Of intensity,measured
R3657 T4244 T4250 arg1Of intensity,using
R3658 T4246 T4245 arg2Of measured,was
R3659 T4246 T4247 arg1Of measured,at
R3660 T4246 T4250 modOf measured,using
R3661 T4249 T4247 arg2Of nm,at
R3662 T4249 T4248 arg1Of nm,450
R3663 T4250 T4256 arg1Of using,(
R3664 T4255 T4250 arg2Of reader,using
R3665 T4255 T4251 arg1Of reader,a
R3666 T4255 T4252 arg1Of reader,Bio-Rad
R3667 T4255 T4253 arg1Of reader,ELISA
R3668 T4255 T4254 arg1Of reader,microplate
R3669 T4258 T4256 arg2Of Laboratories,(
R3670 T4258 T4257 arg1Of Laboratories,Bio-Rad
R3671 T4258 T4259 arg1Of Laboratories,","
R3672 T4260 T4259 arg2Of CA,","
R3673 T4260 T4261 arg1Of CA,","
R3674 T4262 T4261 arg2Of USA,","
R3675 T4263 T4256 arg3Of ),(
R3676 T4265 T4264 arg1Of concentration,The
R3677 T4265 T4266 arg1Of concentration,of
R3678 T4265 T4268 arg1Of concentration,was
R3679 T4265 T4269 arg2Of concentration,determined
R3680 T4265 T4276 arg2Of concentration,expressed
R3681 T4267 T4266 arg2Of protein,of
R3682 T4269 T4270 arg1Of determined,according
R3683 T4269 T4275 arg1Of determined,and
R3684 T4271 T4270 arg2Of to,according
R3685 T4274 T4271 arg2Of curve,to
R3686 T4274 T4272 arg1Of curve,the
R3687 T4274 T4273 arg1Of curve,standard
R3688 T4275 T4268 arg2Of and,was
R3689 T4276 T4275 arg2Of expressed,and
R3690 T4276 T4277 arg1Of expressed,as
R3691 T4278 T4277 arg2Of pg/mg,as
R3692 T4278 T4279 arg1Of pg/mg,of
R3693 T4281 T4279 arg2Of protein,of
R3694 T4281 T4280 arg1Of protein,total
R3709 T4394 T4393 arg1Of Zymography,Gelatine
R3710 T4395 T4396 arg1Of Cells,of
R3711 T4395 T4399 arg1Of Cells,were
R3712 T4395 T4400 arg2Of Cells,homogenized
R3713 T4398 T4396 arg2Of groups,of
R3714 T4398 T4397 arg1Of groups,four
R3715 T4400 T4399 arg2Of homogenized,were
R3716 T4400 T4401 arg1Of homogenized,in
R3717 T4400 T4425 arg1Of homogenized,and
R3718 T4403 T4401 arg2Of buffer,in
R3719 T4403 T4402 arg1Of buffer,lysis
R3720 T4403 T4404 arg1Of buffer,containing
R3721 T4405 T4406 arg1Of 50,mM
R3722 T4407 T4405 arg1Of Tris-HCl,50
R3723 T4407 T4408 arg1Of Tris-HCl,(
R3724 T4407 T4412 arg1Of Tris-HCl,","
R3725 T4409 T4408 arg2Of pH,(
R3726 T4409 T4410 arg1Of pH,7.4
R3727 T4411 T4408 arg3Of ),(
R3728 T4412 T4416 arg1Of ",",","
R3729 T4413 T4414 arg1Of 150,mM
R3730 T4415 T4412 arg2Of NaCl,","
R3731 T4415 T4413 arg1Of NaCl,150
R3732 T4416 T4420 arg1Of ",",","
R3733 T4417 T4418 arg1Of 5,mM
R3734 T4419 T4416 arg2Of CaCl2,","
R3735 T4419 T4417 arg1Of CaCl2,5
R3736 T4420 T4404 arg2Of ",",containing
R3737 T4421 T4422 arg1Of 0.2,mM
R3738 T4423 T4420 arg2Of NaN3,","
R3739 T4423 T4421 arg1Of NaN3,0.2
R3740 T4425 T4424 arg1Of and,","
R3741 T4427 T4426 arg1Of %,0.01
R3742 T4429 T4428 arg1Of Soluble,Triton.
R3743 T4430 T4429 arg1Of extracts,Soluble
R3744 T4430 T4431 arg1Of extracts,were
R3745 T4430 T4432 arg2Of extracts,separated
R3746 T4430 T4436 arg2Of extracts,stored
R3747 T4432 T4435 arg1Of separated,and
R3748 T4434 T4432 arg1Of centrifugation,separated
R3749 T4434 T4433 arg2Of centrifugation,by
R3750 T4435 T4425 arg2Of and,and
R3751 T4435 T4427 arg1Of and,%
R3752 T4435 T4431 arg2Of and,were
R3753 T4436 T4435 arg2Of stored,and
R3754 T4436 T4437 arg1Of stored,at
R3755 T4438 T4437 arg2Of –20°C,at
R3756 T4440 T4439 arg1Of zymography,Gelatin
R3757 T4440 T4441 arg1Of zymography,was
R3758 T4440 T4442 arg2Of zymography,performed
R3759 T4442 T4441 arg2Of performed,was
R3760 T4442 T4443 arg1Of performed,according
R3761 T4444 T4443 arg2Of to,according
R3762 T4446 T4445 arg1Of manufacturer,the
R3763 T4446 T4447 arg2Of manufacturer,'s
R3764 T4448 T4444 arg2Of instructions,to
R3765 T4448 T4447 arg1Of instructions,'s
R3766 T4448 T4449 arg1Of instructions,(
R3767 T4452 T4449 arg2Of Inc,(
R3768 T4452 T4450 arg1Of Inc,Genmed
R3769 T4452 T4451 arg1Of Inc,Scientifics
R3770 T4452 T4453 arg1Of Inc,","
R3771 T4454 T4453 arg2Of MA,","
R3772 T4454 T4455 arg1Of MA,","
R3773 T4456 T4455 arg2Of USA,","
R3774 T4457 T4449 arg3Of ),(
R3775 T4464 T4460 arg1Of extracts,40
R3776 T4464 T4461 arg1Of extracts,μg
R3777 T4464 T4462 arg1Of extracts,cytosolic
R3778 T4464 T4463 arg1Of extracts,protein
R3779 T4464 T4465 arg1Of extracts,were
R3780 T4464 T4466 arg2Of extracts,separated
R3781 T4466 T4458 arg1Of separated,Briefly
R3782 T4466 T4459 arg1Of separated,","
R3783 T4466 T4465 arg2Of separated,were
R3784 T4468 T4466 arg1Of electrophoresis,separated
R3785 T4468 T4467 arg2Of electrophoresis,by
R3786 T4471 T4470 arg1Of proteins,the
R3787 T4471 T4472 arg1Of proteins,were
R3788 T4471 T4473 arg2Of proteins,renatured
R3789 T4471 T4483 arg2Of proteins,incubated
R3790 T4473 T4474 arg1Of renatured,by
R3791 T4473 T4481 arg1Of renatured,and
R3792 T4475 T4474 arg2Of incubation,by
R3793 T4475 T4476 arg1Of incubation,in
R3794 T4477 T4478 arg1Of 2.5,%
R3795 T4480 T4476 arg2Of X-100,in
R3796 T4480 T4477 arg1Of X-100,2.5
R3797 T4480 T4479 arg1Of X-100,Triton
R3798 T4481 T4469 arg1Of and,Then
R3799 T4481 T4472 arg2Of and,were
R3800 T4483 T4481 arg2Of incubated,and
R3801 T4483 T4482 arg1Of incubated,then
R3802 T4483 T4484 arg1Of incubated,in
R3803 T4483 T4487 arg1Of incubated,for
R3804 T4483 T4490 arg1Of incubated,at
R3805 T4483 T4492 modOf incubated,to
R3806 T4486 T4484 arg2Of buffer,in
R3807 T4486 T4485 arg1Of buffer,substrate
R3808 T4489 T4487 arg2Of h,for
R3809 T4489 T4488 arg1Of h,40
R3810 T4491 T4490 arg2Of 37°C,at
R3811 T4493 T4492 arg1Of enable,to
R3812 T4495 T4493 arg2Of MMP9,enable
R3813 T4495 T4494 arg1Of MMP9,the
R3814 T4495 T4497 arg1Of MMP9,cleave
R3815 T4497 T4493 arg3Of cleave,enable
R3816 T4497 T4496 arg1Of cleave,to
R3817 T4499 T4497 arg2Of gelatin,cleave
R3818 T4499 T4498 arg1Of gelatin,the
R3819 T4501 T4500 arg2Of rinsing,After
R3820 T4501 T4502 arg1Of rinsing,in
R3821 T4503 T4502 arg2Of water,in
R3822 T4506 T4501 arg1Of gel,rinsing
R3823 T4506 T4505 arg1Of gel,each
R3824 T4506 T4507 arg1Of gel,was
R3825 T4506 T4508 arg2Of gel,stained
R3826 T4506 T4511 arg1Of gel,blue
R3827 T4506 T4516 arg2Of gel,destained
R3828 T4508 T4509 arg1Of stained,with
R3829 T4508 T4515 arg1Of stained,and
R3830 T4510 T4509 arg2Of Coomassie,with
R3831 T4511 T4508 arg3Of blue,stained
R3832 T4511 T4512 arg1Of blue,for
R3833 T4514 T4512 arg2Of h,for
R3834 T4514 T4513 arg1Of h,1
R3835 T4515 T4500 arg1Of and,After
R3836 T4515 T4504 arg1Of and,","
R3837 T4515 T4507 arg2Of and,was
R3838 T4516 T4515 arg2Of destained,and
R3839 T4516 T4517 arg1Of destained,in
R3840 T4518 T4519 arg1Of 50,%
R3841 T4520 T4517 arg2Of methanol,in
R3842 T4520 T4518 arg1Of methanol,50
R3843 T4522 T4521 arg1Of activities,Proteolytic
R3844 T4522 T4523 arg1Of activities,were
R3845 T4522 T4524 arg2Of activities,showed
R3846 T4524 T4523 arg2Of showed,were
R3847 T4527 T4524 arg1Of bands,showed
R3848 T4527 T4525 arg2Of bands,by
R3849 T4527 T4526 arg1Of bands,clear
R3850 T4527 T4528 arg1Of bands,in
R3851 T4530 T4528 arg2Of gel,in
R3852 T4530 T4529 arg1Of gel,blue
R3853 T4530 T4531 arg1Of gel,which
R3854 T4530 T4532 arg1Of gel,indicates
R3855 T4534 T4532 arg2Of lysis,indicates
R3856 T4534 T4533 arg1Of lysis,the
R3857 T4534 T4535 arg1Of lysis,of
R3858 T4537 T4535 arg2Of substrate,of
R3859 T4537 T4536 arg1Of substrate,the
R3860 T4538 T4539 arg1Of Quantification,of
R3861 T4538 T4543 arg1Of Quantification,was
R3862 T4538 T4544 arg2Of Quantification,performed
R3863 T4542 T4539 arg2Of density,of
R3864 T4542 T4540 arg1Of density,MMP9
R3865 T4542 T4541 arg1Of density,band
R3866 T4544 T4543 arg2Of performed,was
R3867 T4544 T4545 arg1Of performed,with
R3868 T4549 T4545 arg2Of ImageJ,with
R3869 T4549 T4546 arg1Of ImageJ,image
R3870 T4549 T4547 arg1Of ImageJ,analysis
R3871 T4549 T4548 arg1Of ImageJ,program
R4043 T4764 T4763 arg1Of Analysis,Statistical
R4044 T4765 T4766 arg1Of Data,were
R4045 T4765 T4767 arg2Of Data,expressed
R4046 T4765 T4773 arg2Of Data,evaluated
R4047 T4767 T4768 arg1Of expressed,as
R4048 T4767 T4772 arg1Of expressed,and
R4049 T4771 T4768 arg2Of SD,as
R4050 T4771 T4769 arg1Of SD,mean
R4051 T4771 T4770 arg1Of SD,±
R4052 T4772 T4766 arg2Of and,were
R4053 T4773 T4772 arg2Of evaluated,and
R4054 T4775 T4776 arg1Of ANOVA,and
R4055 T4777 T4776 arg2Of LSD,and
R4056 T4780 T4773 arg1Of test,evaluated
R4057 T4780 T4774 arg2Of test,by
R4058 T4780 T4775 arg1Of test,ANOVA
R4059 T4780 T4777 arg1Of test,LSD
R4060 T4780 T4778 arg1Of test,multiple
R4061 T4780 T4779 arg1Of test,comparison
R4062 T4782 T4781 arg1Of values,P
R4063 T4782 T4783 arg1Of values,<
R4064 T4782 T4784 arg1Of values,0.05
R4065 T4782 T4785 arg1Of values,were
R4066 T4782 T4786 arg2Of values,considered
R4067 T4782 T4788 arg1Of values,be
R4068 T4782 T4789 arg1Of values,significant
R4069 T4786 T4785 arg2Of considered,were
R4070 T4788 T4786 arg3Of be,considered
R4071 T4788 T4787 arg1Of be,to
R4072 T4789 T4788 arg2Of significant,be
R4073 T4791 T4790 arg1Of analyses,All
R4074 T4791 T4792 arg1Of analyses,were
R4075 T4791 T4793 arg2Of analyses,performed
R4076 T4793 T4792 arg2Of performed,were
R4077 T4793 T4794 arg1Of performed,by
R4078 T4795 T4794 arg2Of using,by
R4079 T4798 T4795 arg2Of software,using
R4080 T4798 T4796 arg1Of software,SPSS
R4081 T4798 T4797 arg1Of software,18.0
R985 T1242 T1243 arg1Of Culture,and
R986 T1243 T1241 arg1Of and,Primary
R987 T1243 T1245 arg1Of and,of
R988 T1244 T1243 arg2Of Identification,and
R989 T1247 T1245 arg2Of Astrocytes,of
R990 T1247 T1246 arg1Of Astrocytes,Mouse
R991 T1251 T1248 arg1Of mice,Nrf2
R992 T1251 T1249 arg1Of mice,knockout
R993 T1251 T1250 arg1Of mice,ICR
R994 T1251 T1252 arg1Of mice,were
R995 T1251 T1254 arg2Of mice,provided
R996 T1254 T1252 arg2Of provided,were
R997 T1254 T1253 arg1Of provided,kindly
R998 T1259 T1254 arg1Of Kensler,provided
R999 T1259 T1255 arg2Of Kensler,by