pmc-enju-pas Home  

PMC:3317373 / 10566-11434 JSONTXT 19 Projects

Annnotations TAB TSV DIC JSON TextAE

Id Subject Object Predicate Lexical cue
T4395 0-5 NN denotes Cells
T4396 6-8 IN denotes of
T4397 9-13 CD denotes four
T4398 14-20 NN denotes groups
T4399 21-25 VB denotes were
T4400 26-37 VB denotes homogenized
T4401 38-40 IN denotes in
T4402 41-46 NN denotes lysis
T4403 47-53 NN denotes buffer
T4404 54-64 VB denotes containing
T4405 65-67 CD denotes 50
T4406 68-70 NN denotes mM
T4407 71-79 NN denotes Tris-HCl
T4408 80-81 -LRB- denotes (
T4409 81-83 NN denotes pH
T4410 84-87 CD denotes 7.4
T4411 87-88 -RRB- denotes )
T4412 88-89 -COMMA- denotes ,
T4413 90-93 CD denotes 150
T4414 94-96 NN denotes mM
T4415 97-101 NN denotes NaCl
T4416 101-102 -COMMA- denotes ,
T4417 103-104 CD denotes 5
T4418 105-107 NN denotes mM
T4419 108-113 NN denotes CaCl2
T4420 113-114 -COMMA- denotes ,
T4421 115-118 CD denotes 0.2
T4422 119-121 NN denotes mM
T4423 122-126 NN denotes NaN3
T4424 126-127 -COMMA- denotes ,
T4425 128-131 CC denotes and
T4426 132-136 CD denotes 0.01
T4427 136-137 NN denotes %
T4428 138-145 NNP denotes Triton.
T4429 146-153 NNP denotes Soluble
T4430 154-162 NN denotes extracts
T4431 163-167 VB denotes were
T4432 168-177 VB denotes separated
T4433 178-180 IN denotes by
T4434 181-195 NN denotes centrifugation
T4435 196-199 CC denotes and
T4436 200-206 VB denotes stored
T4437 207-209 IN denotes at
T4438 210-215 NN denotes –20°C
T4439 217-224 NN denotes Gelatin
T4440 225-235 NN denotes zymography
T4441 236-239 VB denotes was
T4442 240-249 VB denotes performed
T4443 250-259 VB denotes according
T4444 260-262 TO denotes to
T4445 263-266 DT denotes the
T4446 267-279 NN denotes manufacturer
T4447 279-281 POS denotes 's
T4448 282-294 NN denotes instructions
T4449 295-296 -LRB- denotes (
T4450 296-302 NNP denotes Genmed
T4451 303-314 NNP denotes Scientifics
T4452 315-318 NNP denotes Inc
T4453 318-319 -COMMA- denotes ,
T4454 320-322 NNP denotes MA
T4455 322-323 -COMMA- denotes ,
T4456 324-327 NNP denotes USA
T4457 327-328 -RRB- denotes )
T4458 330-337 RB denotes Briefly
T4459 337-338 -COMMA- denotes ,
T4460 339-341 CD denotes 40
T4461 342-344 NN denotes μg
T4462 345-354 JJ denotes cytosolic
T4463 355-362 NN denotes protein
T4464 363-371 NN denotes extracts
T4465 372-376 VB denotes were
T4466 377-386 VB denotes separated
T4467 387-389 IN denotes by
T4468 390-405 NN denotes electrophoresis
T4469 407-411 RB denotes Then
T4470 412-415 DT denotes the
T4471 416-424 NN denotes proteins
T4472 425-429 VB denotes were
T4473 430-439 VB denotes renatured
T4474 440-442 IN denotes by
T4475 443-453 NN denotes incubation
T4476 454-456 IN denotes in
T4477 457-460 CD denotes 2.5
T4478 460-461 NN denotes %
T4479 462-468 NN denotes Triton
T4480 469-474 NN denotes X-100
T4481 475-478 CC denotes and
T4482 479-483 RB denotes then
T4483 484-493 VB denotes incubated
T4484 494-496 IN denotes in
T4485 497-506 NN denotes substrate
T4486 507-513 NN denotes buffer
T4487 514-517 IN denotes for
T4488 518-520 CD denotes 40
T4489 521-522 NN denotes h
T4490 523-525 IN denotes at
T4491 526-530 NN denotes 37°C
T4492 531-533 TO denotes to
T4493 534-540 VB denotes enable
T4494 541-544 DT denotes the
T4495 545-549 NN denotes MMP9
T4496 550-552 TO denotes to
T4497 553-559 VB denotes cleave
T4498 560-563 DT denotes the
T4499 564-571 NN denotes gelatin
T4500 573-578 IN denotes After
T4501 579-586 VB denotes rinsing
T4502 587-589 IN denotes in
T4503 590-595 NN denotes water
T4504 595-596 -COMMA- denotes ,
T4505 597-601 DT denotes each
T4506 602-605 NN denotes gel
T4507 606-609 VB denotes was
T4508 610-617 VB denotes stained
T4509 618-622 IN denotes with
T4510 623-632 NNP denotes Coomassie
T4511 633-637 JJ denotes blue
T4512 638-641 IN denotes for
T4513 642-643 CD denotes 1
T4514 644-645 NN denotes h
T4515 646-649 CC denotes and
T4516 650-659 VB denotes destained
T4517 660-662 IN denotes in
T4518 663-665 CD denotes 50
T4519 665-666 NN denotes %
T4520 667-675 NN denotes methanol
T4521 677-688 JJ denotes Proteolytic
T4522 689-699 NN denotes activities
T4523 700-704 VB denotes were
T4524 705-711 VB denotes showed
T4525 712-714 IN denotes by
T4526 715-720 JJ denotes clear
T4527 721-726 NN denotes bands
T4528 727-729 IN denotes in
T4529 730-734 JJ denotes blue
T4530 735-738 NN denotes gel
T4531 739-744 WDT denotes which
T4532 745-754 VB denotes indicates
T4533 755-758 DT denotes the
T4534 759-764 NN denotes lysis
T4535 765-767 IN denotes of
T4536 768-771 DT denotes the
T4537 772-781 NN denotes substrate
T4538 783-797 NN denotes Quantification
T4539 798-800 IN denotes of
T4540 801-805 NN denotes MMP9
T4541 806-810 NN denotes band
T4542 811-818 NN denotes density
T4543 819-822 VB denotes was
T4544 823-832 VB denotes performed
T4545 833-837 IN denotes with
T4546 838-843 NN denotes image
T4547 844-852 NN denotes analysis
T4548 853-860 NN denotes program
T4549 861-867 NN denotes ImageJ
R3710 T4395 T4396 arg1Of Cells,of
R3711 T4395 T4399 arg1Of Cells,were
R3712 T4395 T4400 arg2Of Cells,homogenized
R3713 T4398 T4396 arg2Of groups,of
R3714 T4398 T4397 arg1Of groups,four
R3715 T4400 T4399 arg2Of homogenized,were
R3716 T4400 T4401 arg1Of homogenized,in
R3717 T4400 T4425 arg1Of homogenized,and
R3718 T4403 T4401 arg2Of buffer,in
R3719 T4403 T4402 arg1Of buffer,lysis
R3720 T4403 T4404 arg1Of buffer,containing
R3721 T4405 T4406 arg1Of 50,mM
R3722 T4407 T4405 arg1Of Tris-HCl,50
R3723 T4407 T4408 arg1Of Tris-HCl,(
R3724 T4407 T4412 arg1Of Tris-HCl,","
R3725 T4409 T4408 arg2Of pH,(
R3726 T4409 T4410 arg1Of pH,7.4
R3727 T4411 T4408 arg3Of ),(
R3728 T4412 T4416 arg1Of ",",","
R3729 T4413 T4414 arg1Of 150,mM
R3730 T4415 T4412 arg2Of NaCl,","
R3731 T4415 T4413 arg1Of NaCl,150
R3732 T4416 T4420 arg1Of ",",","
R3733 T4417 T4418 arg1Of 5,mM
R3734 T4419 T4416 arg2Of CaCl2,","
R3735 T4419 T4417 arg1Of CaCl2,5
R3736 T4420 T4404 arg2Of ",",containing
R3737 T4421 T4422 arg1Of 0.2,mM
R3738 T4423 T4420 arg2Of NaN3,","
R3739 T4423 T4421 arg1Of NaN3,0.2
R3740 T4425 T4424 arg1Of and,","
R3741 T4427 T4426 arg1Of %,0.01
R3742 T4429 T4428 arg1Of Soluble,Triton.
R3743 T4430 T4429 arg1Of extracts,Soluble
R3744 T4430 T4431 arg1Of extracts,were
R3745 T4430 T4432 arg2Of extracts,separated
R3746 T4430 T4436 arg2Of extracts,stored
R3747 T4432 T4435 arg1Of separated,and
R3748 T4434 T4432 arg1Of centrifugation,separated
R3749 T4434 T4433 arg2Of centrifugation,by
R3750 T4435 T4425 arg2Of and,and
R3751 T4435 T4427 arg1Of and,%
R3752 T4435 T4431 arg2Of and,were
R3753 T4436 T4435 arg2Of stored,and
R3754 T4436 T4437 arg1Of stored,at
R3755 T4438 T4437 arg2Of –20°C,at
R3756 T4440 T4439 arg1Of zymography,Gelatin
R3757 T4440 T4441 arg1Of zymography,was
R3758 T4440 T4442 arg2Of zymography,performed
R3759 T4442 T4441 arg2Of performed,was
R3760 T4442 T4443 arg1Of performed,according
R3761 T4444 T4443 arg2Of to,according
R3762 T4446 T4445 arg1Of manufacturer,the
R3763 T4446 T4447 arg2Of manufacturer,'s
R3764 T4448 T4444 arg2Of instructions,to
R3765 T4448 T4447 arg1Of instructions,'s
R3766 T4448 T4449 arg1Of instructions,(
R3767 T4452 T4449 arg2Of Inc,(
R3768 T4452 T4450 arg1Of Inc,Genmed
R3769 T4452 T4451 arg1Of Inc,Scientifics
R3770 T4452 T4453 arg1Of Inc,","
R3771 T4454 T4453 arg2Of MA,","
R3772 T4454 T4455 arg1Of MA,","
R3773 T4456 T4455 arg2Of USA,","
R3774 T4457 T4449 arg3Of ),(
R3775 T4464 T4460 arg1Of extracts,40
R3776 T4464 T4461 arg1Of extracts,μg
R3777 T4464 T4462 arg1Of extracts,cytosolic
R3778 T4464 T4463 arg1Of extracts,protein
R3779 T4464 T4465 arg1Of extracts,were
R3780 T4464 T4466 arg2Of extracts,separated
R3781 T4466 T4458 arg1Of separated,Briefly
R3782 T4466 T4459 arg1Of separated,","
R3783 T4466 T4465 arg2Of separated,were
R3784 T4468 T4466 arg1Of electrophoresis,separated
R3785 T4468 T4467 arg2Of electrophoresis,by
R3786 T4471 T4470 arg1Of proteins,the
R3787 T4471 T4472 arg1Of proteins,were
R3788 T4471 T4473 arg2Of proteins,renatured
R3789 T4471 T4483 arg2Of proteins,incubated
R3790 T4473 T4474 arg1Of renatured,by
R3791 T4473 T4481 arg1Of renatured,and
R3792 T4475 T4474 arg2Of incubation,by
R3793 T4475 T4476 arg1Of incubation,in
R3794 T4477 T4478 arg1Of 2.5,%
R3795 T4480 T4476 arg2Of X-100,in
R3796 T4480 T4477 arg1Of X-100,2.5
R3797 T4480 T4479 arg1Of X-100,Triton
R3798 T4481 T4469 arg1Of and,Then
R3799 T4481 T4472 arg2Of and,were
R3800 T4483 T4481 arg2Of incubated,and
R3801 T4483 T4482 arg1Of incubated,then
R3802 T4483 T4484 arg1Of incubated,in
R3803 T4483 T4487 arg1Of incubated,for
R3804 T4483 T4490 arg1Of incubated,at
R3805 T4483 T4492 modOf incubated,to
R3806 T4486 T4484 arg2Of buffer,in
R3807 T4486 T4485 arg1Of buffer,substrate
R3808 T4489 T4487 arg2Of h,for
R3809 T4489 T4488 arg1Of h,40
R3810 T4491 T4490 arg2Of 37°C,at
R3811 T4493 T4492 arg1Of enable,to
R3812 T4495 T4493 arg2Of MMP9,enable
R3813 T4495 T4494 arg1Of MMP9,the
R3814 T4495 T4497 arg1Of MMP9,cleave
R3815 T4497 T4493 arg3Of cleave,enable
R3816 T4497 T4496 arg1Of cleave,to
R3817 T4499 T4497 arg2Of gelatin,cleave
R3818 T4499 T4498 arg1Of gelatin,the
R3819 T4501 T4500 arg2Of rinsing,After
R3820 T4501 T4502 arg1Of rinsing,in
R3821 T4503 T4502 arg2Of water,in
R3822 T4506 T4501 arg1Of gel,rinsing
R3823 T4506 T4505 arg1Of gel,each
R3824 T4506 T4507 arg1Of gel,was
R3825 T4506 T4508 arg2Of gel,stained
R3826 T4506 T4511 arg1Of gel,blue
R3827 T4506 T4516 arg2Of gel,destained
R3828 T4508 T4509 arg1Of stained,with
R3829 T4508 T4515 arg1Of stained,and
R3830 T4510 T4509 arg2Of Coomassie,with
R3831 T4511 T4508 arg3Of blue,stained
R3832 T4511 T4512 arg1Of blue,for
R3833 T4514 T4512 arg2Of h,for
R3834 T4514 T4513 arg1Of h,1
R3835 T4515 T4500 arg1Of and,After
R3836 T4515 T4504 arg1Of and,","
R3837 T4515 T4507 arg2Of and,was
R3838 T4516 T4515 arg2Of destained,and
R3839 T4516 T4517 arg1Of destained,in
R3840 T4518 T4519 arg1Of 50,%
R3841 T4520 T4517 arg2Of methanol,in
R3842 T4520 T4518 arg1Of methanol,50
R3843 T4522 T4521 arg1Of activities,Proteolytic
R3844 T4522 T4523 arg1Of activities,were
R3845 T4522 T4524 arg2Of activities,showed
R3846 T4524 T4523 arg2Of showed,were
R3847 T4527 T4524 arg1Of bands,showed
R3848 T4527 T4525 arg2Of bands,by
R3849 T4527 T4526 arg1Of bands,clear
R3850 T4527 T4528 arg1Of bands,in
R3851 T4530 T4528 arg2Of gel,in
R3852 T4530 T4529 arg1Of gel,blue
R3853 T4530 T4531 arg1Of gel,which
R3854 T4530 T4532 arg1Of gel,indicates
R3855 T4534 T4532 arg2Of lysis,indicates
R3856 T4534 T4533 arg1Of lysis,the
R3857 T4534 T4535 arg1Of lysis,of
R3858 T4537 T4535 arg2Of substrate,of
R3859 T4537 T4536 arg1Of substrate,the
R3860 T4538 T4539 arg1Of Quantification,of
R3861 T4538 T4543 arg1Of Quantification,was
R3862 T4538 T4544 arg2Of Quantification,performed
R3863 T4542 T4539 arg2Of density,of
R3864 T4542 T4540 arg1Of density,MMP9
R3865 T4542 T4541 arg1Of density,band
R3866 T4544 T4543 arg2Of performed,was
R3867 T4544 T4545 arg1Of performed,with
R3868 T4549 T4545 arg2Of ImageJ,with
R3869 T4549 T4546 arg1Of ImageJ,image
R3870 T4549 T4547 arg1Of ImageJ,analysis
R3871 T4549 T4548 arg1Of ImageJ,program