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PMC:3317373 / 10541-11434 JSONTXT 19 Projects

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Id Subject Object Predicate Lexical cue
T4392 0-3 CD denotes 2.8
T4393 5-13 NN denotes Gelatine
T4394 14-24 NN denotes Zymography
T4395 25-30 NN denotes Cells
T4396 31-33 IN denotes of
T4397 34-38 CD denotes four
T4398 39-45 NN denotes groups
T4399 46-50 VB denotes were
T4400 51-62 VB denotes homogenized
T4401 63-65 IN denotes in
T4402 66-71 NN denotes lysis
T4403 72-78 NN denotes buffer
T4404 79-89 VB denotes containing
T4405 90-92 CD denotes 50
T4406 93-95 NN denotes mM
T4407 96-104 NN denotes Tris-HCl
T4408 105-106 -LRB- denotes (
T4409 106-108 NN denotes pH
T4410 109-112 CD denotes 7.4
T4411 112-113 -RRB- denotes )
T4412 113-114 -COMMA- denotes ,
T4413 115-118 CD denotes 150
T4414 119-121 NN denotes mM
T4415 122-126 NN denotes NaCl
T4416 126-127 -COMMA- denotes ,
T4417 128-129 CD denotes 5
T4418 130-132 NN denotes mM
T4419 133-138 NN denotes CaCl2
T4420 138-139 -COMMA- denotes ,
T4421 140-143 CD denotes 0.2
T4422 144-146 NN denotes mM
T4423 147-151 NN denotes NaN3
T4424 151-152 -COMMA- denotes ,
T4425 153-156 CC denotes and
T4426 157-161 CD denotes 0.01
T4427 161-162 NN denotes %
T4428 163-170 NNP denotes Triton.
T4429 171-178 NNP denotes Soluble
T4430 179-187 NN denotes extracts
T4431 188-192 VB denotes were
T4432 193-202 VB denotes separated
T4433 203-205 IN denotes by
T4434 206-220 NN denotes centrifugation
T4435 221-224 CC denotes and
T4436 225-231 VB denotes stored
T4437 232-234 IN denotes at
T4438 235-240 NN denotes –20°C
T4439 242-249 NN denotes Gelatin
T4440 250-260 NN denotes zymography
T4441 261-264 VB denotes was
T4442 265-274 VB denotes performed
T4443 275-284 VB denotes according
T4444 285-287 TO denotes to
T4445 288-291 DT denotes the
T4446 292-304 NN denotes manufacturer
T4447 304-306 POS denotes 's
T4448 307-319 NN denotes instructions
T4449 320-321 -LRB- denotes (
T4450 321-327 NNP denotes Genmed
T4451 328-339 NNP denotes Scientifics
T4452 340-343 NNP denotes Inc
T4453 343-344 -COMMA- denotes ,
T4454 345-347 NNP denotes MA
T4455 347-348 -COMMA- denotes ,
T4456 349-352 NNP denotes USA
T4457 352-353 -RRB- denotes )
T4458 355-362 RB denotes Briefly
T4459 362-363 -COMMA- denotes ,
T4460 364-366 CD denotes 40
T4461 367-369 NN denotes μg
T4462 370-379 JJ denotes cytosolic
T4463 380-387 NN denotes protein
T4464 388-396 NN denotes extracts
T4465 397-401 VB denotes were
T4466 402-411 VB denotes separated
T4467 412-414 IN denotes by
T4468 415-430 NN denotes electrophoresis
T4469 432-436 RB denotes Then
T4470 437-440 DT denotes the
T4471 441-449 NN denotes proteins
T4472 450-454 VB denotes were
T4473 455-464 VB denotes renatured
T4474 465-467 IN denotes by
T4475 468-478 NN denotes incubation
T4476 479-481 IN denotes in
T4477 482-485 CD denotes 2.5
T4478 485-486 NN denotes %
T4479 487-493 NN denotes Triton
T4480 494-499 NN denotes X-100
T4481 500-503 CC denotes and
T4482 504-508 RB denotes then
T4483 509-518 VB denotes incubated
T4484 519-521 IN denotes in
T4485 522-531 NN denotes substrate
T4486 532-538 NN denotes buffer
T4487 539-542 IN denotes for
T4488 543-545 CD denotes 40
T4489 546-547 NN denotes h
T4490 548-550 IN denotes at
T4491 551-555 NN denotes 37°C
T4492 556-558 TO denotes to
T4493 559-565 VB denotes enable
T4494 566-569 DT denotes the
T4495 570-574 NN denotes MMP9
T4496 575-577 TO denotes to
T4497 578-584 VB denotes cleave
T4498 585-588 DT denotes the
T4499 589-596 NN denotes gelatin
T4500 598-603 IN denotes After
T4501 604-611 VB denotes rinsing
T4502 612-614 IN denotes in
T4503 615-620 NN denotes water
T4504 620-621 -COMMA- denotes ,
T4505 622-626 DT denotes each
T4506 627-630 NN denotes gel
T4507 631-634 VB denotes was
T4508 635-642 VB denotes stained
T4509 643-647 IN denotes with
T4510 648-657 NNP denotes Coomassie
T4511 658-662 JJ denotes blue
T4512 663-666 IN denotes for
T4513 667-668 CD denotes 1
T4514 669-670 NN denotes h
T4515 671-674 CC denotes and
T4516 675-684 VB denotes destained
T4517 685-687 IN denotes in
T4518 688-690 CD denotes 50
T4519 690-691 NN denotes %
T4520 692-700 NN denotes methanol
T4521 702-713 JJ denotes Proteolytic
T4522 714-724 NN denotes activities
T4523 725-729 VB denotes were
T4524 730-736 VB denotes showed
T4525 737-739 IN denotes by
T4526 740-745 JJ denotes clear
T4527 746-751 NN denotes bands
T4528 752-754 IN denotes in
T4529 755-759 JJ denotes blue
T4530 760-763 NN denotes gel
T4531 764-769 WDT denotes which
T4532 770-779 VB denotes indicates
T4533 780-783 DT denotes the
T4534 784-789 NN denotes lysis
T4535 790-792 IN denotes of
T4536 793-796 DT denotes the
T4537 797-806 NN denotes substrate
T4538 808-822 NN denotes Quantification
T4539 823-825 IN denotes of
T4540 826-830 NN denotes MMP9
T4541 831-835 NN denotes band
T4542 836-843 NN denotes density
T4543 844-847 VB denotes was
T4544 848-857 VB denotes performed
T4545 858-862 IN denotes with
T4546 863-868 NN denotes image
T4547 869-877 NN denotes analysis
T4548 878-885 NN denotes program
T4549 886-892 NN denotes ImageJ
R3709 T4394 T4393 arg1Of Zymography,Gelatine
R3710 T4395 T4396 arg1Of Cells,of
R3711 T4395 T4399 arg1Of Cells,were
R3712 T4395 T4400 arg2Of Cells,homogenized
R3713 T4398 T4396 arg2Of groups,of
R3714 T4398 T4397 arg1Of groups,four
R3715 T4400 T4399 arg2Of homogenized,were
R3716 T4400 T4401 arg1Of homogenized,in
R3717 T4400 T4425 arg1Of homogenized,and
R3718 T4403 T4401 arg2Of buffer,in
R3719 T4403 T4402 arg1Of buffer,lysis
R3720 T4403 T4404 arg1Of buffer,containing
R3721 T4405 T4406 arg1Of 50,mM
R3722 T4407 T4405 arg1Of Tris-HCl,50
R3723 T4407 T4408 arg1Of Tris-HCl,(
R3724 T4407 T4412 arg1Of Tris-HCl,","
R3725 T4409 T4408 arg2Of pH,(
R3726 T4409 T4410 arg1Of pH,7.4
R3727 T4411 T4408 arg3Of ),(
R3728 T4412 T4416 arg1Of ",",","
R3729 T4413 T4414 arg1Of 150,mM
R3730 T4415 T4412 arg2Of NaCl,","
R3731 T4415 T4413 arg1Of NaCl,150
R3732 T4416 T4420 arg1Of ",",","
R3733 T4417 T4418 arg1Of 5,mM
R3734 T4419 T4416 arg2Of CaCl2,","
R3735 T4419 T4417 arg1Of CaCl2,5
R3736 T4420 T4404 arg2Of ",",containing
R3737 T4421 T4422 arg1Of 0.2,mM
R3738 T4423 T4420 arg2Of NaN3,","
R3739 T4423 T4421 arg1Of NaN3,0.2
R3740 T4425 T4424 arg1Of and,","
R3741 T4427 T4426 arg1Of %,0.01
R3742 T4429 T4428 arg1Of Soluble,Triton.
R3743 T4430 T4429 arg1Of extracts,Soluble
R3744 T4430 T4431 arg1Of extracts,were
R3745 T4430 T4432 arg2Of extracts,separated
R3746 T4430 T4436 arg2Of extracts,stored
R3747 T4432 T4435 arg1Of separated,and
R3748 T4434 T4432 arg1Of centrifugation,separated
R3749 T4434 T4433 arg2Of centrifugation,by
R3750 T4435 T4425 arg2Of and,and
R3751 T4435 T4427 arg1Of and,%
R3752 T4435 T4431 arg2Of and,were
R3753 T4436 T4435 arg2Of stored,and
R3754 T4436 T4437 arg1Of stored,at
R3755 T4438 T4437 arg2Of –20°C,at
R3756 T4440 T4439 arg1Of zymography,Gelatin
R3757 T4440 T4441 arg1Of zymography,was
R3758 T4440 T4442 arg2Of zymography,performed
R3759 T4442 T4441 arg2Of performed,was
R3760 T4442 T4443 arg1Of performed,according
R3761 T4444 T4443 arg2Of to,according
R3762 T4446 T4445 arg1Of manufacturer,the
R3763 T4446 T4447 arg2Of manufacturer,'s
R3764 T4448 T4444 arg2Of instructions,to
R3765 T4448 T4447 arg1Of instructions,'s
R3766 T4448 T4449 arg1Of instructions,(
R3767 T4452 T4449 arg2Of Inc,(
R3768 T4452 T4450 arg1Of Inc,Genmed
R3769 T4452 T4451 arg1Of Inc,Scientifics
R3770 T4452 T4453 arg1Of Inc,","
R3771 T4454 T4453 arg2Of MA,","
R3772 T4454 T4455 arg1Of MA,","
R3773 T4456 T4455 arg2Of USA,","
R3774 T4457 T4449 arg3Of ),(
R3775 T4464 T4460 arg1Of extracts,40
R3776 T4464 T4461 arg1Of extracts,μg
R3777 T4464 T4462 arg1Of extracts,cytosolic
R3778 T4464 T4463 arg1Of extracts,protein
R3779 T4464 T4465 arg1Of extracts,were
R3780 T4464 T4466 arg2Of extracts,separated
R3781 T4466 T4458 arg1Of separated,Briefly
R3782 T4466 T4459 arg1Of separated,","
R3783 T4466 T4465 arg2Of separated,were
R3784 T4468 T4466 arg1Of electrophoresis,separated
R3785 T4468 T4467 arg2Of electrophoresis,by
R3786 T4471 T4470 arg1Of proteins,the
R3787 T4471 T4472 arg1Of proteins,were
R3788 T4471 T4473 arg2Of proteins,renatured
R3789 T4471 T4483 arg2Of proteins,incubated
R3790 T4473 T4474 arg1Of renatured,by
R3791 T4473 T4481 arg1Of renatured,and
R3792 T4475 T4474 arg2Of incubation,by
R3793 T4475 T4476 arg1Of incubation,in
R3794 T4477 T4478 arg1Of 2.5,%
R3795 T4480 T4476 arg2Of X-100,in
R3796 T4480 T4477 arg1Of X-100,2.5
R3797 T4480 T4479 arg1Of X-100,Triton
R3798 T4481 T4469 arg1Of and,Then
R3799 T4481 T4472 arg2Of and,were
R3800 T4483 T4481 arg2Of incubated,and
R3801 T4483 T4482 arg1Of incubated,then
R3802 T4483 T4484 arg1Of incubated,in
R3803 T4483 T4487 arg1Of incubated,for
R3804 T4483 T4490 arg1Of incubated,at
R3805 T4483 T4492 modOf incubated,to
R3806 T4486 T4484 arg2Of buffer,in
R3807 T4486 T4485 arg1Of buffer,substrate
R3808 T4489 T4487 arg2Of h,for
R3809 T4489 T4488 arg1Of h,40
R3810 T4491 T4490 arg2Of 37°C,at
R3811 T4493 T4492 arg1Of enable,to
R3812 T4495 T4493 arg2Of MMP9,enable
R3813 T4495 T4494 arg1Of MMP9,the
R3814 T4495 T4497 arg1Of MMP9,cleave
R3815 T4497 T4493 arg3Of cleave,enable
R3816 T4497 T4496 arg1Of cleave,to
R3817 T4499 T4497 arg2Of gelatin,cleave
R3818 T4499 T4498 arg1Of gelatin,the
R3819 T4501 T4500 arg2Of rinsing,After
R3820 T4501 T4502 arg1Of rinsing,in
R3821 T4503 T4502 arg2Of water,in
R3822 T4506 T4501 arg1Of gel,rinsing
R3823 T4506 T4505 arg1Of gel,each
R3824 T4506 T4507 arg1Of gel,was
R3825 T4506 T4508 arg2Of gel,stained
R3826 T4506 T4511 arg1Of gel,blue
R3827 T4506 T4516 arg2Of gel,destained
R3828 T4508 T4509 arg1Of stained,with
R3829 T4508 T4515 arg1Of stained,and
R3830 T4510 T4509 arg2Of Coomassie,with
R3831 T4511 T4508 arg3Of blue,stained
R3832 T4511 T4512 arg1Of blue,for
R3833 T4514 T4512 arg2Of h,for
R3834 T4514 T4513 arg1Of h,1
R3835 T4515 T4500 arg1Of and,After
R3836 T4515 T4504 arg1Of and,","
R3837 T4515 T4507 arg2Of and,was
R3838 T4516 T4515 arg2Of destained,and
R3839 T4516 T4517 arg1Of destained,in
R3840 T4518 T4519 arg1Of 50,%
R3841 T4520 T4517 arg2Of methanol,in
R3842 T4520 T4518 arg1Of methanol,50
R3843 T4522 T4521 arg1Of activities,Proteolytic
R3844 T4522 T4523 arg1Of activities,were
R3845 T4522 T4524 arg2Of activities,showed
R3846 T4524 T4523 arg2Of showed,were
R3847 T4527 T4524 arg1Of bands,showed
R3848 T4527 T4525 arg2Of bands,by
R3849 T4527 T4526 arg1Of bands,clear
R3850 T4527 T4528 arg1Of bands,in
R3851 T4530 T4528 arg2Of gel,in
R3852 T4530 T4529 arg1Of gel,blue
R3853 T4530 T4531 arg1Of gel,which
R3854 T4530 T4532 arg1Of gel,indicates
R3855 T4534 T4532 arg2Of lysis,indicates
R3856 T4534 T4533 arg1Of lysis,the
R3857 T4534 T4535 arg1Of lysis,of
R3858 T4537 T4535 arg2Of substrate,of
R3859 T4537 T4536 arg1Of substrate,the
R3860 T4538 T4539 arg1Of Quantification,of
R3861 T4538 T4543 arg1Of Quantification,was
R3862 T4538 T4544 arg2Of Quantification,performed
R3863 T4542 T4539 arg2Of density,of
R3864 T4542 T4540 arg1Of density,MMP9
R3865 T4542 T4541 arg1Of density,band
R3866 T4544 T4543 arg2Of performed,was
R3867 T4544 T4545 arg1Of performed,with
R3868 T4549 T4545 arg2Of ImageJ,with
R3869 T4549 T4546 arg1Of ImageJ,image
R3870 T4549 T4547 arg1Of ImageJ,analysis
R3871 T4549 T4548 arg1Of ImageJ,program