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PMC:3279418 / 30568-30970 JSONTXT 12 Projects

Annnotations TAB TSV DIC JSON TextAE

Id Subject Object Predicate Lexical cue
T11671 0-7 NN denotes Primers
T11672 8-11 CC denotes and
T11673 12-18 NN denotes probes
T11674 19-23 VB denotes were
T11675 24-33 VB denotes purchased
T11676 34-38 IN denotes from
T11677 39-46 NNP denotes Applied
T11678 47-58 NNP denotes Biosystems.
T11679 59-66 NNP denotes Reverse
T11680 67-80 NN denotes transcription
T11681 81-84 VB denotes was
T11682 85-94 VB denotes performed
T11683 95-97 IN denotes at
T11684 98-102 NN denotes 42°C
T11685 103-106 IN denotes for
T11686 107-109 CD denotes 60
T11687 110-117 NN denotes minutes
T11688 118-122 IN denotes with
T11689 123-126 DT denotes the
T11690 127-132 JJ denotes final
T11691 133-145 NN denotes denaturation
T11692 146-150 NN denotes step
T11693 151-153 IN denotes at
T11694 154-158 NN denotes 90°C
T11695 159-162 IN denotes for
T11696 163-164 CD denotes 5
T11697 165-172 NN denotes minutes
T11698 173-175 IN denotes in
T11699 176-178 CD denotes 30
T11700 179-181 NN denotes µl
T11701 182-192 VB denotes containing
T11702 193-196 CD denotes 0.5
T11703 197-199 NN denotes µg
T11704 200-202 IN denotes of
T11705 203-208 JJ denotes total
T11706 209-213 NN denotes RNA.
T11707 214-219 NN denotes dNTPs
T11708 219-220 -COMMA- denotes ,
T11709 221-226 NN denotes oligo
T11710 226-227 -LRB- denotes (
T11711 227-229 NN denotes dT
T11712 229-230 -RRB- denotes )
T11713 230-232 CD denotes 15
T11714 233-239 NN denotes primer
T11715 239-240 -COMMA- denotes ,
T11716 241-252 JJ denotes recombinant
T11717 253-259 NNP denotes RNasin
T11718 260-272 NNP denotes Ribonuclease
T11719 273-282 NNP denotes Inhibitor
T11720 282-283 -COMMA- denotes ,
T11721 284-287 CC denotes and
T11722 288-293 NN denotes M-MLV
T11723 294-301 JJ denotes Reverse
T11724 302-315 NN denotes Transcriptase
T11725 316-317 -LRB- denotes (
T11726 317-320 DT denotes all
T11727 321-325 IN denotes from
T11728 326-333 NNP denotes Promega
T11729 333-334 -COMMA- denotes ,
T11730 335-342 NNP denotes Madison
T11731 342-343 -COMMA- denotes ,
T11732 344-346 NNP denotes WI
T11733 346-347 -RRB- denotes )
T11734 348-352 VB denotes were
T11735 353-357 VB denotes used
T11736 358-367 VB denotes according
T11737 368-370 TO denotes to
T11738 371-374 DT denotes the
T11739 375-387 NN denotes manufacturer
T11740 387-389 POS denotes 's
T11741 390-401 NN denotes instruction
R9710 T11671 T11672 arg1Of Primers,and
R9711 T11672 T11674 arg1Of and,were
R9712 T11672 T11675 arg2Of and,purchased
R9713 T11673 T11672 arg2Of probes,and
R9714 T11675 T11674 arg2Of purchased,were
R9715 T11675 T11676 arg1Of purchased,from
R9716 T11675 T11721 arg1Of purchased,and
R9717 T11679 T11677 arg1Of Reverse,Applied
R9718 T11679 T11678 arg1Of Reverse,Biosystems.
R9719 T11679 T11701 arg1Of Reverse,containing
R9720 T11680 T11681 arg1Of transcription,was
R9721 T11680 T11682 arg2Of transcription,performed
R9722 T11682 T11681 arg2Of performed,was
R9723 T11682 T11683 arg1Of performed,at
R9724 T11682 T11685 arg1Of performed,for
R9725 T11682 T11688 arg1Of performed,with
R9726 T11684 T11683 arg2Of 42°C,at
R9727 T11687 T11685 arg2Of minutes,for
R9728 T11687 T11686 arg1Of minutes,60
R9729 T11692 T11688 arg2Of step,with
R9730 T11692 T11689 arg1Of step,the
R9731 T11692 T11690 arg1Of step,final
R9732 T11692 T11691 arg1Of step,denaturation
R9733 T11692 T11693 arg1Of step,at
R9734 T11692 T11695 arg1Of step,for
R9735 T11692 T11698 arg1Of step,in
R9736 T11694 T11693 arg2Of 90°C,at
R9737 T11697 T11695 arg2Of minutes,for
R9738 T11697 T11696 arg1Of minutes,5
R9739 T11700 T11698 arg2Of µl,in
R9740 T11700 T11699 arg1Of µl,30
R9741 T11701 T11681 modOf containing,was
R9742 T11703 T11701 arg2Of µg,containing
R9743 T11703 T11702 arg1Of µg,0.5
R9744 T11703 T11704 arg1Of µg,of
R9745 T11707 T11704 arg2Of dNTPs,of
R9746 T11707 T11705 arg1Of dNTPs,total
R9747 T11707 T11706 arg1Of dNTPs,RNA.
R9748 T11707 T11708 arg1Of dNTPs,","
R9749 T11709 T11708 arg2Of oligo,","
R9750 T11709 T11710 arg1Of oligo,(
R9751 T11711 T11710 arg2Of dT,(
R9752 T11712 T11710 arg3Of ),(
R9753 T11714 T11676 arg2Of primer,from
R9754 T11714 T11679 arg1Of primer,Reverse
R9755 T11714 T11713 arg1Of primer,15
R9756 T11714 T11715 arg1Of primer,","
R9757 T11719 T11715 arg2Of Inhibitor,","
R9758 T11719 T11716 arg1Of Inhibitor,recombinant
R9759 T11719 T11717 arg1Of Inhibitor,RNasin
R9760 T11719 T11718 arg1Of Inhibitor,Ribonuclease
R9761 T11721 T11720 arg1Of and,","
R9762 T11724 T11722 arg1Of Transcriptase,M-MLV
R9763 T11724 T11723 arg1Of Transcriptase,Reverse
R9764 T11724 T11725 arg1Of Transcriptase,(
R9765 T11724 T11734 arg1Of Transcriptase,were
R9766 T11724 T11735 arg2Of Transcriptase,used
R9767 T11726 T11725 arg2Of all,(
R9768 T11726 T11727 arg1Of all,from
R9769 T11726 T11729 arg1Of all,","
R9770 T11728 T11727 arg2Of Promega,from
R9771 T11730 T11729 arg2Of Madison,","
R9772 T11730 T11731 arg1Of Madison,","
R9773 T11732 T11731 arg2Of WI,","
R9774 T11733 T11725 arg3Of ),(
R9775 T11735 T11721 arg2Of used,and
R9776 T11735 T11734 arg2Of used,were
R9777 T11735 T11736 arg1Of used,according
R9778 T11737 T11736 arg2Of to,according
R9779 T11739 T11738 arg1Of manufacturer,the
R9780 T11739 T11740 arg2Of manufacturer,'s
R9781 T11741 T11737 arg2Of instruction,to
R9782 T11741 T11740 arg1Of instruction,'s