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PMC:3279418 / 30214-31448 JSONTXT 15 Projects

Annnotations TAB TSV DIC JSON TextAE

Id Subject Object Predicate Lexical cue
T11602 0-3 NN denotes RNA
T11603 4-14 NN denotes expression
T11604 15-17 IN denotes by
T11605 18-22 NN denotes BMDC
T11606 23-27 NN denotes BMDC
T11607 28-32 VB denotes were
T11608 33-41 VB denotes cultured
T11609 42-44 IN denotes at
T11610 45-48 CD denotes 106
T11611 49-57 NN denotes cells/mL
T11612 58-60 IN denotes in
T11613 61-63 CD denotes 10
T11614 64-66 NN denotes mL
T11615 67-69 IN denotes of
T11616 70-79 NN denotes RPMI-1640
T11617 80-88 JJ denotes complete
T11618 89-95 NN denotes medium
T11619 96-98 IN denotes in
T11620 99-102 DT denotes the
T11621 103-111 NN denotes presence
T11622 112-114 CC denotes or
T11623 115-122 NN denotes absence
T11624 123-125 IN denotes of
T11625 126-129 CD denotes 100
T11626 130-135 NN denotes ng/mL
T11627 136-139 NN denotes LPS
T11628 140-142 CC denotes or
T11629 143-145 CD denotes 25
T11630 146-151 NN denotes µg/mL
T11631 152-156 NN denotes poly
T11632 157-158 CD denotes I
T11633 158-159 -COLON- denotes :
T11634 159-161 NNP denotes C.
T11635 162-167 IN denotes After
T11636 168-169 CD denotes 1
T11637 170-173 CC denotes and
T11638 174-175 CD denotes 2
T11639 176-181 NN denotes hours
T11640 181-182 -COMMA- denotes ,
T11641 183-186 NN denotes RNA
T11642 187-190 VB denotes was
T11643 191-199 VB denotes isolated
T11644 200-204 IN denotes with
T11645 205-216 NN denotes TRI-reagent
T11646 217-218 -LRB- denotes (
T11647 218-223 NN denotes Sigma
T11648 223-224 -RRB- denotes )
T11649 225-234 VB denotes according
T11650 235-237 TO denotes to
T11651 238-241 DT denotes the
T11652 242-254 NN denotes manufacturer
T11653 254-256 POS denotes 's
T11654 257-269 NN denotes instructions
T11655 271-274 DT denotes The
T11656 275-285 NN denotes expression
T11657 286-288 IN denotes of
T11658 289-292 NN denotes Il6
T11659 292-293 -COMMA- denotes ,
T11660 294-301 NN denotes Il12p40
T11661 301-302 -COMMA- denotes ,
T11662 303-308 NN denotes Gmcsf
T11663 308-309 -COMMA- denotes ,
T11664 310-313 CC denotes and
T11665 314-321 NN denotes Sharpin
T11666 322-326 NN denotes mRNA
T11667 327-330 VB denotes was
T11668 331-341 VB denotes determined
T11669 342-344 IN denotes by
T11670 345-352 NN denotes qRT-PCR
T11671 354-361 NN denotes Primers
T11672 362-365 CC denotes and
T11673 366-372 NN denotes probes
T11674 373-377 VB denotes were
T11675 378-387 VB denotes purchased
T11676 388-392 IN denotes from
T11677 393-400 NNP denotes Applied
T11678 401-412 NNP denotes Biosystems.
T11679 413-420 NNP denotes Reverse
T11680 421-434 NN denotes transcription
T11681 435-438 VB denotes was
T11682 439-448 VB denotes performed
T11683 449-451 IN denotes at
T11684 452-456 NN denotes 42°C
T11685 457-460 IN denotes for
T11686 461-463 CD denotes 60
T11687 464-471 NN denotes minutes
T11688 472-476 IN denotes with
T11689 477-480 DT denotes the
T11690 481-486 JJ denotes final
T11691 487-499 NN denotes denaturation
T11692 500-504 NN denotes step
T11693 505-507 IN denotes at
T11694 508-512 NN denotes 90°C
T11695 513-516 IN denotes for
T11696 517-518 CD denotes 5
T11697 519-526 NN denotes minutes
T11698 527-529 IN denotes in
T11699 530-532 CD denotes 30
T11700 533-535 NN denotes µl
T11701 536-546 VB denotes containing
T11702 547-550 CD denotes 0.5
T11703 551-553 NN denotes µg
T11704 554-556 IN denotes of
T11705 557-562 JJ denotes total
T11706 563-567 NN denotes RNA.
T11707 568-573 NN denotes dNTPs
T11708 573-574 -COMMA- denotes ,
T11709 575-580 NN denotes oligo
T11710 580-581 -LRB- denotes (
T11711 581-583 NN denotes dT
T11712 583-584 -RRB- denotes )
T11713 584-586 CD denotes 15
T11714 587-593 NN denotes primer
T11715 593-594 -COMMA- denotes ,
T11716 595-606 JJ denotes recombinant
T11717 607-613 NNP denotes RNasin
T11718 614-626 NNP denotes Ribonuclease
T11719 627-636 NNP denotes Inhibitor
T11720 636-637 -COMMA- denotes ,
T11721 638-641 CC denotes and
T11722 642-647 NN denotes M-MLV
T11723 648-655 JJ denotes Reverse
T11724 656-669 NN denotes Transcriptase
T11725 670-671 -LRB- denotes (
T11726 671-674 DT denotes all
T11727 675-679 IN denotes from
T11728 680-687 NNP denotes Promega
T11729 687-688 -COMMA- denotes ,
T11730 689-696 NNP denotes Madison
T11731 696-697 -COMMA- denotes ,
T11732 698-700 NNP denotes WI
T11733 700-701 -RRB- denotes )
T11734 702-706 VB denotes were
T11735 707-711 VB denotes used
T11736 712-721 VB denotes according
T11737 722-724 TO denotes to
T11738 725-728 DT denotes the
T11739 729-741 NN denotes manufacturer
T11740 741-743 POS denotes 's
T11741 744-755 NN denotes instruction
T11742 757-764 JJ denotes Reverse
T11743 765-778 NN denotes transcription
T11744 779-782 VB denotes was
T11745 783-787 VB denotes done
T11746 788-790 IN denotes in
T11747 791-792 DT denotes a
T11748 793-800 NN denotes PTC-200
T11749 801-808 NNP denotes Peltier
T11750 809-816 NNP denotes Thermal
T11751 817-823 NNP denotes Cycler
T11752 824-825 -LRB- denotes (
T11753 825-827 NNP denotes MJ
T11754 828-836 NNP denotes Research
T11755 836-837 -COMMA- denotes ,
T11756 838-847 NNP denotes Watertown
T11757 847-848 -COMMA- denotes ,
T11758 849-851 JJ denotes MA
T11759 851-852 -RRB- denotes )
T11760 852-859 NN denotes . qRT-P
T11761 859-862 VB denotes CR
T11762 862-871 VB denotes was perfo
T11763 871-873 IN denotes rm
T11764 873-876 NNP denotes ed
T11765 876-881 NNP denotes in AB
T11766 881-885 CD denotes I Pr
T11767 885-893 NNP denotes ism 7700
T11768 894-903 NNP denotes Sequence
T11769 903-909 NNP denotes Detect
T11770 909-913 IN denotes ion
T11771 913-920 NNP denotes System
T11772 920-924 NNP denotes with
T11773 925-935 NN denotes TaqMan® Ge
T11774 935-941 NN denotes ne Exp
T11775 941-942 -LRB- denotes r
T11776 942-949 NNP denotes ession
T11777 949-959 NNP denotes Assays (Ap
T11778 959-960 -COMMA- denotes p
T11779 960-966 NNP denotes lied B
T11780 966-970 NNP denotes iosy
T11781 970-971 -COMMA- denotes s
T11782 971-973 NNP denotes te
T11783 973-974 -RRB- denotes m
T11784 974-977 IN denotes s,
T11785 977-982 NN denotes Foste
T11786 982-986 NN denotes r Ci
T11787 986-987 -COMMA- denotes t
T11788 987-990 NN denotes y,
T11789 990-991 -COMMA- denotes C
T11790 991-998 NN denotes A) for
T11791 998-999 -COMMA- denotes m
T11792 999-1003 NNP denotes ouse
T11793 1004-1005 -COMMA- denotes A
T11794 1005-1010 NNP denotes ctb,
T11795 1010-1011 -COMMA- denotes I
T11796 1011-1014 CC denotes L6,
T11797 1015-1022 NNP denotes IL12p40
T11798 1022-1031 VB denotes , Ifnβ, G
T11799 1031-1033 TO denotes mc
T11800 1033-1036 DT denotes sf,
T11801 1037-1049 NN denotes and Sharpin
T11802 1049-1051 POS denotes ac
T11803 1051-1059 NN denotes cording
T11804 1091-1094 DT denotes The
T11805 1095-1105 JJ denotes endogenous
T11806 1106-1114 NN denotes standard
T11807 1115-1118 IN denotes for
T11808 1119-1132 NN denotes normalization
T11809 1133-1135 IN denotes of
T11810 1136-1139 DT denotes the
T11811 1140-1146 NN denotes target
T11812 1147-1151 NN denotes gene
T11813 1152-1155 VB denotes was
T11814 1156-1163 JJ denotes β-actin
T11815 1165-1173 JJ denotes Relative
T11816 1174-1178 NN denotes gene
T11817 1179-1189 NN denotes expression
T11818 1190-1193 VB denotes was
T11819 1194-1204 VB denotes calculated
T11820 1205-1210 VB denotes using
T11821 1211-1214 DT denotes the
T11822 1215-1221 NN denotes 2−ΔΔCt
T11823 1222-1228 NN denotes method
T11824 1229-1230 -LRB- denotes [
T11825 1230-1232 CD denotes 56
T11826 1232-1233 -RRB- denotes ]
R9640 T11603 T11602 arg1Of expression,RNA
R9641 T11603 T11604 arg1Of expression,by
R9642 T11605 T11604 arg2Of BMDC,by
R9643 T11606 T11607 arg1Of BMDC,were
R9644 T11606 T11608 arg2Of BMDC,cultured
R9645 T11608 T11607 arg2Of cultured,were
R9646 T11608 T11609 arg1Of cultured,at
R9647 T11608 T11612 arg1Of cultured,in
R9648 T11608 T11619 arg1Of cultured,in
R9649 T11608 T11635 arg1Of cultured,After
R9650 T11611 T11609 arg2Of cells/mL,at
R9651 T11611 T11610 arg1Of cells/mL,106
R9652 T11614 T11612 arg2Of mL,in
R9653 T11614 T11613 arg1Of mL,10
R9654 T11614 T11615 arg1Of mL,of
R9655 T11618 T11615 arg2Of medium,of
R9656 T11618 T11616 arg1Of medium,RPMI-1640
R9657 T11618 T11617 arg1Of medium,complete
R9658 T11621 T11622 arg1Of presence,or
R9659 T11622 T11619 arg2Of or,in
R9660 T11622 T11620 arg1Of or,the
R9661 T11622 T11624 arg1Of or,of
R9662 T11622 T11633 arg1Of or,:
R9663 T11623 T11622 arg2Of absence,or
R9664 T11625 T11626 arg1Of 100,ng/mL
R9665 T11627 T11625 arg1Of LPS,100
R9666 T11627 T11628 arg1Of LPS,or
R9667 T11628 T11624 arg2Of or,of
R9668 T11631 T11628 arg2Of poly,or
R9669 T11631 T11629 arg1Of poly,25
R9670 T11631 T11630 arg1Of poly,µg/mL
R9671 T11631 T11632 arg1Of poly,I
R9672 T11634 T11633 arg2Of C.,:
R9673 T11636 T11637 arg1Of 1,and
R9674 T11638 T11637 arg2Of 2,and
R9675 T11639 T11635 arg2Of hours,After
R9676 T11639 T11636 arg1Of hours,1
R9677 T11639 T11638 arg1Of hours,2
R9678 T11641 T11642 arg1Of RNA,was
R9679 T11641 T11643 arg2Of RNA,isolated
R9680 T11643 T11607 modOf isolated,were
R9681 T11643 T11640 arg1Of isolated,","
R9682 T11643 T11642 arg2Of isolated,was
R9683 T11643 T11644 arg1Of isolated,with
R9684 T11643 T11649 arg1Of isolated,according
R9685 T11645 T11644 arg2Of TRI-reagent,with
R9686 T11645 T11646 arg1Of TRI-reagent,(
R9687 T11647 T11646 arg2Of Sigma,(
R9688 T11648 T11646 arg3Of ),(
R9689 T11650 T11649 arg2Of to,according
R9690 T11652 T11651 arg1Of manufacturer,the
R9691 T11652 T11653 arg2Of manufacturer,'s
R9692 T11654 T11650 arg2Of instructions,to
R9693 T11654 T11653 arg1Of instructions,'s
R9694 T11656 T11655 arg1Of expression,The
R9695 T11656 T11657 arg1Of expression,of
R9696 T11656 T11667 arg1Of expression,was
R9697 T11656 T11668 arg2Of expression,determined
R9698 T11658 T11659 arg1Of Il6,","
R9699 T11659 T11661 arg1Of ",",","
R9700 T11660 T11659 arg2Of Il12p40,","
R9701 T11661 T11664 arg1Of ",",and
R9702 T11662 T11661 arg2Of Gmcsf,","
R9703 T11664 T11657 arg2Of and,of
R9704 T11664 T11663 arg1Of and,","
R9705 T11666 T11664 arg2Of mRNA,and
R9706 T11666 T11665 arg1Of mRNA,Sharpin
R9707 T11668 T11667 arg2Of determined,was
R9708 T11670 T11668 arg1Of qRT-PCR,determined
R9709 T11670 T11669 arg2Of qRT-PCR,by
R9710 T11671 T11672 arg1Of Primers,and
R9711 T11672 T11674 arg1Of and,were
R9712 T11672 T11675 arg2Of and,purchased
R9713 T11673 T11672 arg2Of probes,and
R9714 T11675 T11674 arg2Of purchased,were
R9715 T11675 T11676 arg1Of purchased,from
R9716 T11675 T11721 arg1Of purchased,and
R9717 T11679 T11677 arg1Of Reverse,Applied
R9718 T11679 T11678 arg1Of Reverse,Biosystems.
R9719 T11679 T11701 arg1Of Reverse,containing
R9720 T11680 T11681 arg1Of transcription,was
R9721 T11680 T11682 arg2Of transcription,performed
R9722 T11682 T11681 arg2Of performed,was
R9723 T11682 T11683 arg1Of performed,at
R9724 T11682 T11685 arg1Of performed,for
R9725 T11682 T11688 arg1Of performed,with
R9726 T11684 T11683 arg2Of 42°C,at
R9727 T11687 T11685 arg2Of minutes,for
R9728 T11687 T11686 arg1Of minutes,60
R9729 T11692 T11688 arg2Of step,with
R9730 T11692 T11689 arg1Of step,the
R9731 T11692 T11690 arg1Of step,final
R9732 T11692 T11691 arg1Of step,denaturation
R9733 T11692 T11693 arg1Of step,at
R9734 T11692 T11695 arg1Of step,for
R9735 T11692 T11698 arg1Of step,in
R9736 T11694 T11693 arg2Of 90°C,at
R9737 T11697 T11695 arg2Of minutes,for
R9738 T11697 T11696 arg1Of minutes,5
R9739 T11700 T11698 arg2Of µl,in
R9740 T11700 T11699 arg1Of µl,30
R9741 T11701 T11681 modOf containing,was
R9742 T11703 T11701 arg2Of µg,containing
R9743 T11703 T11702 arg1Of µg,0.5
R9744 T11703 T11704 arg1Of µg,of
R9745 T11707 T11704 arg2Of dNTPs,of
R9746 T11707 T11705 arg1Of dNTPs,total
R9747 T11707 T11706 arg1Of dNTPs,RNA.
R9748 T11707 T11708 arg1Of dNTPs,","
R9749 T11709 T11708 arg2Of oligo,","
R9750 T11709 T11710 arg1Of oligo,(
R9751 T11711 T11710 arg2Of dT,(
R9752 T11712 T11710 arg3Of ),(
R9753 T11714 T11676 arg2Of primer,from
R9754 T11714 T11679 arg1Of primer,Reverse
R9755 T11714 T11713 arg1Of primer,15
R9756 T11714 T11715 arg1Of primer,","
R9757 T11719 T11715 arg2Of Inhibitor,","
R9758 T11719 T11716 arg1Of Inhibitor,recombinant
R9759 T11719 T11717 arg1Of Inhibitor,RNasin
R9760 T11719 T11718 arg1Of Inhibitor,Ribonuclease
R9761 T11721 T11720 arg1Of and,","
R9762 T11724 T11722 arg1Of Transcriptase,M-MLV
R9763 T11724 T11723 arg1Of Transcriptase,Reverse
R9764 T11724 T11725 arg1Of Transcriptase,(
R9765 T11724 T11734 arg1Of Transcriptase,were
R9766 T11724 T11735 arg2Of Transcriptase,used
R9767 T11726 T11725 arg2Of all,(
R9768 T11726 T11727 arg1Of all,from
R9769 T11726 T11729 arg1Of all,","
R9770 T11728 T11727 arg2Of Promega,from
R9771 T11730 T11729 arg2Of Madison,","
R9772 T11730 T11731 arg1Of Madison,","
R9773 T11732 T11731 arg2Of WI,","
R9774 T11733 T11725 arg3Of ),(
R9775 T11735 T11721 arg2Of used,and
R9776 T11735 T11734 arg2Of used,were
R9777 T11735 T11736 arg1Of used,according
R9778 T11737 T11736 arg2Of to,according
R9779 T11739 T11738 arg1Of manufacturer,the
R9780 T11739 T11740 arg2Of manufacturer,'s
R9781 T11741 T11737 arg2Of instruction,to
R9782 T11741 T11740 arg1Of instruction,'s
R9783 T11743 T11742 arg1Of transcription,Reverse
R9784 T11743 T11744 arg1Of transcription,was
R9785 T11743 T11745 arg2Of transcription,done
R9786 T11745 T11744 arg2Of done,was
R9787 T11745 T11746 arg1Of done,in
R9788 T11751 T11746 arg2Of Cycler,in
R9789 T11751 T11747 arg1Of Cycler,a
R9790 T11751 T11748 arg1Of Cycler,PTC-200
R9791 T11751 T11749 arg1Of Cycler,Peltier
R9792 T11751 T11750 arg1Of Cycler,Thermal
R9793 T11751 T11752 arg1Of Cycler,(
R9794 T11754 T11753 arg1Of Research,MJ
R9795 T11754 T11755 arg1Of Research,","
R9796 T11755 T11752 arg2Of ",",(
R9797 T11755 T11757 arg1Of ",",","
R9798 T11755 T11758 arg1Of ",",MA
R9799 T11756 T11755 arg2Of Watertown,","
R9800 T11759 T11752 arg3Of ),(
R9801 T11760 T11761 arg1Of . qRT-P,CR
R9802 T11760 T11762 arg2Of . qRT-P,was perfo
R9803 T11762 T11744 modOf was perfo,was
R9804 T11762 T11761 arg2Of was perfo,CR
R9805 T11762 T11763 arg1Of was perfo,rm
R9806 T11762 T11798 arg1Of was perfo,", Ifnβ, G"
R9807 T11769 T11763 arg2Of Detect,rm
R9808 T11769 T11764 arg1Of Detect,ed
R9809 T11769 T11765 arg1Of Detect,in AB
R9810 T11769 T11766 arg1Of Detect,I Pr
R9811 T11769 T11767 arg1Of Detect,ism 7700
R9812 T11769 T11768 arg1Of Detect,Sequence
R9813 T11769 T11770 arg1Of Detect,ion
R9814 T11769 T11784 arg1Of Detect,"s, "
R9815 T11772 T11771 arg1Of with,System
R9816 T11774 T11770 arg2Of ne Exp,ion
R9817 T11774 T11772 arg1Of ne Exp,with
R9818 T11774 T11773 arg1Of ne Exp,TaqMan® Ge
R9819 T11774 T11775 arg1Of ne Exp,r
R9820 T11777 T11775 arg2Of Assays (Ap,r
R9821 T11777 T11776 arg1Of Assays (Ap,ession
R9822 T11777 T11778 arg1Of Assays (Ap,p
R9823 T11780 T11778 arg2Of iosy,p
R9824 T11780 T11779 arg1Of iosy,lied B
R9825 T11780 T11781 arg1Of iosy,s
R9826 T11782 T11781 arg2Of te,s
R9827 T11783 T11775 arg3Of m,r
R9828 T11786 T11785 arg1Of r Ci,Foste
R9829 T11786 T11787 arg1Of r Ci,t
R9830 T11787 T11789 arg1Of t,C
R9831 T11788 T11787 arg2Of "y, ",t
R9832 T11789 T11791 arg1Of C,m
R9833 T11790 T11789 arg2Of A) for ,C
R9834 T11791 T11793 arg1Of m,A
R9835 T11792 T11791 arg2Of ouse,m
R9836 T11793 T11796 arg1Of A,"L6,"
R9837 T11794 T11793 arg2Of "ctb, ",A
R9838 T11796 T11784 arg2Of "L6,","s, "
R9839 T11796 T11795 arg1Of "L6,",I
R9840 T11797 T11796 arg2Of IL12p40,"L6,"
R9841 T11799 T11798 arg2Of mc,", Ifnβ, G"
R9842 T11801 T11800 arg1Of and Sharpin ,"sf,"
R9843 T11801 T11802 arg2Of and Sharpin ,ac
R9844 T11803 T11799 arg2Of cording ,mc
R9845 T11803 T11802 arg1Of cording ,ac
R9846 T11806 T11804 arg1Of standard,The
R9847 T11806 T11805 arg1Of standard,endogenous
R9848 T11806 T11807 arg1Of standard,for
R9849 T11806 T11813 arg1Of standard,was
R9850 T11806 T11814 arg1Of standard,β-actin
R9851 T11808 T11807 arg2Of normalization,for
R9852 T11808 T11809 arg1Of normalization,of
R9853 T11812 T11809 arg2Of gene,of
R9854 T11812 T11810 arg1Of gene,the
R9855 T11812 T11811 arg1Of gene,target
R9856 T11814 T11813 arg2Of β-actin,was
R9857 T11817 T11815 arg1Of expression,Relative
R9858 T11817 T11816 arg1Of expression,gene
R9859 T11817 T11818 arg1Of expression,was
R9860 T11817 T11819 arg2Of expression,calculated
R9861 T11819 T11818 arg2Of calculated,was
R9862 T11819 T11820 modOf calculated,using
R9863 T11820 T11824 arg1Of using,[
R9864 T11823 T11820 arg2Of method,using
R9865 T11823 T11821 arg1Of method,the
R9866 T11823 T11822 arg1Of method,2−ΔΔCt
R9867 T11825 T11824 arg2Of 56,[
R9868 T11826 T11824 arg3Of ],[