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PMC:3148254 JSONTXT 20 Projects

Annnotations TAB TSV DIC JSON TextAE

Id Subject Object Predicate Lexical cue
T425 0-7 NN denotes HOIL-1L
T426 8-19 VB denotes Interacting
T427 20-27 NN denotes Protein
T428 28-29 -LRB- denotes (
T429 29-33 NN denotes HOIP
T430 33-34 -RRB- denotes )
T431 35-37 VB denotes Is
T432 38-47 JJ denotes Essential
T433 48-51 IN denotes for
T434 52-56 NN denotes CD40
T435 57-66 NN denotes Signaling
T436 115-119 NN denotes CD40
T437 120-122 VB denotes is
T438 123-124 DT denotes a
T439 125-129 NN denotes cell
T440 130-137 NN denotes surface
T441 138-146 NN denotes receptor
T442 147-156 JJ denotes important
T443 157-159 IN denotes in
T444 160-163 DT denotes the
T445 164-174 NN denotes activation
T446 175-177 IN denotes of
T447 178-196 JJ denotes antigen-presenting
T448 197-202 NN denotes cells
T449 203-209 IN denotes during
T450 210-216 JJ denotes immune
T451 217-226 NN denotes responses
T452 228-230 IN denotes In
T453 231-242 NN denotes macrophages
T454 243-246 CC denotes and
T455 247-256 JJ denotes dendritic
T456 257-262 NN denotes cells
T457 262-263 -COMMA- denotes ,
T458 264-274 NN denotes engagement
T459 275-277 IN denotes of
T460 278-282 NN denotes CD40
T461 283-285 IN denotes by
T462 286-289 PRP-DOLLAR- denotes its
T463 290-296 NN denotes ligand
T464 297-302 NN denotes CD154
T465 303-311 VB denotes provides
T466 312-319 NN denotes signals
T467 320-328 JJ denotes critical
T468 329-332 IN denotes for
T469 333-347 JJ denotes anti-microbial
T470 348-351 CC denotes and
T471 352-353 NN denotes T
T472 354-367 JJ denotes cell-mediated
T473 368-374 JJ denotes immune
T474 375-384 NN denotes responses
T475 384-385 -COMMA- denotes ,
T476 386-398 RB denotes respectively
T477 400-402 IN denotes In
T478 403-404 NN denotes B
T479 405-410 NN denotes cells
T480 410-411 -COMMA- denotes ,
T481 412-416 NN denotes CD40
T482 417-426 NN denotes signaling
T483 427-430 VB denotes has
T484 431-432 DT denotes a
T485 433-438 JJ denotes major
T486 439-443 NN denotes role
T487 444-446 IN denotes in
T488 447-457 VB denotes regulating
T489 458-462 NN denotes cell
T490 463-476 NN denotes proliferation
T491 476-477 -COMMA- denotes ,
T492 478-486 NN denotes antibody
T493 487-497 NN denotes production
T494 497-498 -COMMA- denotes ,
T495 499-502 CC denotes and
T496 503-509 NN denotes memory
T497 510-511 NN denotes B
T498 512-516 NN denotes cell
T499 517-528 NN denotes development
T500 530-534 NN denotes CD40
T501 535-545 NN denotes engagement
T502 546-553 VB denotes results
T503 554-556 IN denotes in
T504 557-560 DT denotes the
T505 561-570 NN denotes formation
T506 571-573 IN denotes of
T507 574-575 DT denotes a
T508 576-595 JJ denotes receptor-associated
T509 596-603 NN denotes complex
T510 604-608 WDT denotes that
T511 609-617 VB denotes mediates
T512 618-628 NN denotes activation
T513 629-631 IN denotes of
T514 632-637 NN denotes NF-κB
T515 637-638 -COMMA- denotes ,
T516 639-655 JJ denotes stress-activated
T517 656-663 NN denotes protein
T518 664-671 NN denotes kinases
T519 671-672 -COMMA- denotes ,
T520 673-676 CC denotes and
T521 677-682 JJ denotes other
T522 683-692 VB denotes signaling
T523 693-702 NN denotes molecules
T524 704-711 RB denotes However
T525 711-712 -COMMA- denotes ,
T526 713-716 DT denotes the
T527 717-727 NN denotes mechanisms
T528 728-732 WDT denotes that
T529 733-737 VB denotes link
T530 738-742 NN denotes CD40
T531 743-745 TO denotes to
T532 746-751 DT denotes these
T533 752-761 NN denotes signaling
T534 762-768 NN denotes events
T535 769-773 VB denotes have
T536 774-778 VB denotes been
T537 779-783 RB denotes only
T538 784-793 RB denotes partially
T539 794-807 VB denotes characterized
T540 809-814 VB denotes Known
T541 815-825 NN denotes components
T542 826-828 IN denotes of
T543 829-832 DT denotes the
T544 833-837 NN denotes CD40
T545 838-847 NN denotes signaling
T546 848-855 NN denotes complex
T547 856-863 VB denotes include
T548 864-871 NN denotes members
T549 872-874 IN denotes of
T550 875-878 DT denotes the
T551 879-882 NN denotes TNF
T552 883-902 JJ denotes receptor-associated
T553 903-909 NN denotes factor
T554 910-911 -LRB- denotes (
T555 911-915 NN denotes TRAF
T556 915-916 -RRB- denotes )
T557 917-923 NN denotes family
T558 924-926 IN denotes of
T559 927-935 NN denotes proteins
T560 937-939 PRP denotes We
T561 940-950 RB denotes previously
T562 951-957 VB denotes showed
T563 958-962 IN denotes that
T564 963-966 DT denotes the
T565 967-971 NN denotes TRAF
T566 972-978 NN denotes family
T567 979-985 NN denotes member
T568 986-991 NN denotes TRAF2
T569 992-1000 VB denotes mediates
T570 1001-1012 NN denotes recruitment
T571 1013-1015 IN denotes of
T572 1016-1035 JJ denotes HOIL-1L-interacting
T573 1036-1043 NN denotes protein
T574 1044-1045 -LRB- denotes (
T575 1045-1049 NN denotes HOIP
T576 1049-1050 -RRB- denotes )
T577 1051-1053 TO denotes to
T578 1054-1057 DT denotes the
T579 1058-1069 JJ denotes cytoplasmic
T580 1070-1076 NN denotes domain
T581 1077-1079 IN denotes of
T582 1080-1084 NN denotes CD40
T583 1084-1085 -COMMA- denotes ,
T584 1086-1096 VB denotes suggesting
T585 1097-1101 IN denotes that
T586 1102-1106 NN denotes HOIP
T587 1107-1110 VB denotes has
T588 1111-1112 DT denotes a
T589 1113-1117 NN denotes role
T590 1118-1120 IN denotes in
T591 1121-1124 DT denotes the
T592 1125-1129 NN denotes CD40
T593 1130-1139 NN denotes signaling
T594 1140-1147 NN denotes pathway
T595 1149-1151 TO denotes To
T596 1152-1161 VB denotes determine
T597 1162-1165 DT denotes the
T598 1166-1170 NN denotes role
T599 1171-1173 IN denotes of
T600 1174-1178 NN denotes HOIP
T601 1179-1181 IN denotes in
T602 1182-1186 NN denotes CD40
T603 1187-1196 NN denotes signaling
T604 1196-1197 -COMMA- denotes ,
T605 1198-1200 PRP denotes we
T606 1201-1205 VB denotes used
T607 1206-1213 JJ denotes somatic
T608 1214-1218 NN denotes cell
T609 1219-1223 NN denotes gene
T610 1224-1233 VB denotes targeting
T611 1234-1236 TO denotes to
T612 1237-1245 VB denotes generate
T613 1246-1251 NN denotes mouse
T614 1252-1253 NN denotes B
T615 1254-1258 NN denotes cell
T616 1259-1264 NN denotes lines
T617 1265-1274 JJ denotes deficient
T618 1275-1277 IN denotes in
T619 1278-1282 NN denotes HOIP
T620 1284-1286 PRP denotes We
T621 1287-1292 VB denotes found
T622 1293-1297 IN denotes that
T623 1298-1301 DT denotes the
T624 1302-1314 JJ denotes CD40-induced
T625 1315-1327 NN denotes upregulation
T626 1328-1330 IN denotes of
T627 1331-1335 NN denotes CD80
T628 1336-1339 CC denotes and
T629 1340-1350 NN denotes activation
T630 1351-1353 IN denotes of
T631 1354-1362 NN denotes germline
T632 1363-1377 NN denotes immunoglobulin
T633 1378-1385 NN denotes epsilon
T634 1386-1399 NN denotes transcription
T635 1400-1404 VB denotes were
T636 1405-1414 JJ denotes defective
T637 1415-1417 IN denotes in
T638 1418-1432 JJ denotes HOIP-deficient
T639 1433-1438 NN denotes cells
T640 1440-1442 PRP denotes We
T641 1443-1447 RB denotes also
T642 1448-1453 VB denotes found
T643 1454-1458 IN denotes that
T644 1459-1462 DT denotes the
T645 1463-1476 JJ denotes CD40-mediated
T646 1477-1487 NN denotes activation
T647 1488-1490 IN denotes of
T648 1491-1496 NN denotes NF-κB
T649 1497-1500 CC denotes and
T650 1501-1506 NN denotes c-Jun
T651 1507-1513 NN denotes kinase
T652 1514-1517 VB denotes was
T653 1518-1526 JJ denotes impaired
T654 1528-1539 NN denotes Recruitment
T655 1540-1542 IN denotes of
T656 1543-1546 NN denotes IκB
T657 1547-1553 NN denotes kinase
T658 1554-1562 NN denotes proteins
T659 1563-1565 TO denotes to
T660 1566-1569 DT denotes the
T661 1570-1574 NN denotes CD40
T662 1575-1584 NN denotes signaling
T663 1585-1592 NN denotes complex
T664 1593-1596 VB denotes was
T665 1597-1609 JJ denotes undetectable
T666 1610-1612 IN denotes in
T667 1613-1627 JJ denotes HOIP-deficient
T668 1628-1633 NN denotes cells
T669 1633-1634 -COMMA- denotes ,
T670 1635-1646 RB denotes potentially
T671 1647-1657 VB denotes explaining
T672 1658-1661 DT denotes the
T673 1662-1668 NN denotes defect
T674 1669-1671 IN denotes in
T675 1672-1677 NN denotes NF-κB
T676 1678-1688 NN denotes activation
T677 1690-1701 NN denotes Restoration
T678 1702-1704 IN denotes of
T679 1705-1709 NN denotes HOIP
T680 1710-1720 NN denotes expression
T681 1721-1729 VB denotes reversed
T682 1730-1733 DT denotes the
T683 1734-1741 NN denotes defects
T684 1742-1744 IN denotes in
T685 1745-1753 JJ denotes cellular
T686 1754-1764 NN denotes activation
T687 1765-1768 CC denotes and
T688 1769-1778 NN denotes signaling
T689 1780-1785 DT denotes These
T690 1786-1793 NN denotes results
T691 1794-1800 VB denotes reveal
T692 1801-1805 NN denotes HOIP
T693 1806-1808 IN denotes as
T694 1809-1810 DT denotes a
T695 1811-1814 JJ denotes key
T696 1815-1824 NN denotes component
T697 1825-1827 IN denotes of
T698 1828-1831 DT denotes the
T699 1832-1836 NN denotes CD40
T700 1837-1846 NN denotes signaling
T701 1847-1854 NN denotes pathway
T1725 1870-1874 NN denotes CD40
T1726 1875-1884 NN denotes signaling
T1727 1885-1887 IN denotes in
T1728 1888-1900 JJ denotes professional
T1729 1901-1919 JJ denotes antigen-presenting
T1730 1920-1925 NN denotes cells
T1731 1925-1926 -COMMA- denotes ,
T1732 1927-1936 VB denotes including
T1733 1937-1938 NN denotes B
T1734 1939-1944 NN denotes cells
T1735 1944-1945 -COMMA- denotes ,
T1736 1946-1957 NN denotes macrophages
T1737 1957-1958 -COMMA- denotes ,
T1738 1959-1962 CC denotes and
T1739 1963-1972 JJ denotes dendritic
T1740 1973-1978 NN denotes cells
T1741 1978-1979 -COMMA- denotes ,
T1742 1980-1982 VB denotes is
T1743 1983-1991 JJ denotes critical
T1744 1992-1995 IN denotes for
T1745 1996-1999 DT denotes the
T1746 2000-2009 JJ denotes efficient
T1747 2010-2020 NN denotes activation
T1748 2021-2023 IN denotes of
T1749 2024-2031 JJ denotes humoral
T1750 2032-2035 CC denotes and
T1751 2036-2049 JJ denotes cell-mediated
T1752 2050-2056 JJ denotes immune
T1753 2057-2066 NN denotes responses
T1754 2067-2068 -LRB- denotes [
T1755 2068-2069 CD denotes 1
T1756 2069-2070 -RRB- denotes ]
T1757 2070-2071 -COMMA- denotes ,
T1758 2072-2073 -LRB- denotes [
T1759 2073-2074 CD denotes 2
T1760 2074-2075 -RRB- denotes ]
T1761 2075-2076 -COMMA- denotes ,
T1762 2077-2078 -LRB- denotes [
T1763 2078-2079 CD denotes 3
T1764 2079-2080 -RRB- denotes ]
T1765 2082-2086 NN denotes CD40
T1766 2087-2096 NN denotes signaling
T1767 2097-2099 VB denotes is
T1768 2100-2109 VB denotes activated
T1769 2110-2112 IN denotes in
T1770 2113-2114 DT denotes a
T1771 2115-2116 NN denotes T
T1772 2117-2131 JJ denotes cell-dependent
T1773 2132-2138 NN denotes manner
T1774 2138-2139 -COMMA- denotes ,
T1775 2140-2142 IN denotes as
T1776 2143-2146 DT denotes the
T1777 2147-2153 NN denotes ligand
T1778 2154-2157 IN denotes for
T1779 2158-2162 NN denotes CD40
T1780 2162-2163 -COMMA- denotes ,
T1781 2164-2169 NN denotes CD154
T1782 2169-2170 -COMMA- denotes ,
T1783 2171-2173 VB denotes is
T1784 2174-2183 VB denotes expressed
T1785 2184-2193 RB denotes primarily
T1786 2194-2196 IN denotes by
T1787 2197-2206 VB denotes activated
T1788 2207-2208 NN denotes T
T1789 2209-2214 NN denotes cells
T1790 2216-2220 NN denotes CD40
T1791 2221-2231 NN denotes engagement
T1792 2232-2237 VB denotes leads
T1793 2238-2240 TO denotes to
T1794 2241-2244 DT denotes the
T1795 2245-2255 NN denotes activation
T1796 2256-2258 IN denotes of
T1797 2259-2266 JJ denotes various
T1798 2267-2276 VB denotes signaling
T1799 2277-2286 NN denotes molecules
T1800 2286-2287 -COMMA- denotes ,
T1801 2288-2297 VB denotes including
T1802 2298-2314 JJ denotes stress-activated
T1803 2315-2322 NN denotes protein
T1804 2323-2330 NN denotes kinases
T1805 2331-2334 CC denotes and
T1806 2335-2338 DT denotes the
T1807 2339-2352 NN denotes transcription
T1808 2353-2359 NN denotes factor
T1809 2360-2365 NN denotes NF-κB
T1810 2365-2366 -COMMA- denotes ,
T1811 2367-2372 WDT denotes which
T1812 2373-2383 VB denotes upregulate
T1813 2384-2387 DT denotes the
T1814 2388-2398 NN denotes expression
T1815 2399-2401 IN denotes of
T1816 2402-2411 NN denotes cytokines
T1817 2412-2415 CC denotes and
T1818 2416-2421 JJ denotes other
T1819 2422-2429 NN denotes factors
T1820 2430-2434 WDT denotes that
T1821 2435-2442 VB denotes promote
T1822 2443-2449 JJ denotes immune
T1823 2450-2459 NN denotes responses
T1824 2461-2464 DT denotes The
T1825 2465-2474 NN denotes mechanism
T1826 2475-2477 IN denotes by
T1827 2478-2483 WDT denotes which
T1828 2484-2488 NN denotes CD40
T1829 2489-2496 VB denotes induces
T1830 2497-2502 DT denotes these
T1831 2503-2512 NN denotes signaling
T1832 2513-2521 NN denotes pathways
T1833 2522-2525 VB denotes has
T1834 2526-2529 RB denotes not
T1835 2530-2534 VB denotes been
T1836 2535-2545 RB denotes completely
T1837 2546-2553 VB denotes defined
T1838 2555-2558 DT denotes The
T1839 2559-2570 JJ denotes cytoplasmic
T1840 2571-2577 NN denotes domain
T1841 2578-2580 IN denotes of
T1842 2581-2585 NN denotes CD40
T1843 2586-2590 VB denotes does
T1844 2591-2594 RB denotes not
T1845 2595-2601 VB denotes appear
T1846 2602-2604 TO denotes to
T1847 2605-2609 VB denotes have
T1848 2610-2619 JJ denotes intrinsic
T1849 2620-2629 JJ denotes enzymatic
T1850 2630-2638 NN denotes activity
T1851 2638-2639 -COMMA- denotes ,
T1852 2640-2643 CC denotes but
T1853 2644-2646 VB denotes is
T1854 2647-2651 JJ denotes able
T1855 2652-2654 TO denotes to
T1856 2655-2662 VB denotes mediate
T1857 2663-2672 NN denotes signaling
T1858 2673-2680 IN denotes through
T1859 2681-2684 DT denotes the
T1860 2685-2696 NN denotes recruitment
T1861 2697-2699 IN denotes of
T1862 2700-2707 JJ denotes several
T1863 2708-2721 JJ denotes intracellular
T1864 2722-2730 NN denotes proteins
T1865 2732-2739 NN denotes Members
T1866 2740-2742 IN denotes of
T1867 2743-2746 DT denotes the
T1868 2747-2750 NN denotes TNF
T1869 2751-2770 JJ denotes receptor-associated
T1870 2771-2777 NN denotes factor
T1871 2778-2779 -LRB- denotes (
T1872 2779-2783 NN denotes TRAF
T1873 2783-2784 -RRB- denotes )
T1874 2785-2791 NN denotes family
T1875 2791-2792 -COMMA- denotes ,
T1876 2793-2802 VB denotes including
T1877 2803-2808 NN denotes TRAF1
T1878 2808-2809 -COMMA- denotes ,
T1879 2810-2815 NN denotes TRAF2
T1880 2815-2816 -COMMA- denotes ,
T1881 2817-2822 NN denotes TRAF3
T1882 2822-2823 -COMMA- denotes ,
T1883 2824-2827 CC denotes and
T1884 2828-2833 NN denotes TRAF6
T1885 2833-2834 -COMMA- denotes ,
T1886 2835-2841 VB denotes appear
T1887 2842-2844 TO denotes to
T1888 2845-2847 VB denotes be
T1889 2848-2860 RB denotes particularly
T1890 2861-2870 JJ denotes important
T1891 2871-2874 IN denotes for
T1892 2875-2878 DT denotes the
T1893 2879-2889 NN denotes initiation
T1894 2890-2893 CC denotes and
T1895 2894-2904 NN denotes regulation
T1896 2905-2907 IN denotes of
T1897 2908-2912 NN denotes CD40
T1898 2913-2922 NN denotes signaling
T1899 2923-2924 -LRB- denotes [
T1900 2924-2925 CD denotes 4
T1901 2925-2926 -RRB- denotes ]
T1902 2928-2933 DT denotes These
T1903 2934-2942 NN denotes proteins
T1904 2943-2951 VB denotes function
T1905 2952-2954 IN denotes in
T1906 2955-2959 NN denotes part
T1907 2960-2962 IN denotes as
T1908 2963-2970 NN denotes adaptor
T1909 2971-2980 NN denotes molecules
T1910 2980-2981 -COMMA- denotes ,
T1911 2982-2989 VB denotes binding
T1912 2990-2992 TO denotes to
T1913 2993-2996 DT denotes the
T1914 2997-3008 JJ denotes cytoplasmic
T1915 3009-3013 NN denotes tail
T1916 3014-3016 IN denotes of
T1917 3017-3021 NN denotes CD40
T1918 3022-3025 CC denotes and
T1919 3026-3036 VB denotes recruiting
T1920 3037-3042 JJ denotes other
T1921 3043-3051 NN denotes proteins
T1922 3052-3054 TO denotes to
T1923 3055-3058 DT denotes the
T1924 3059-3078 JJ denotes receptor-associated
T1925 3079-3086 NN denotes complex
T1926 3088-3092 DT denotes Some
T1927 3093-3095 IN denotes of
T1928 3096-3099 DT denotes the
T1929 3100-3105 NN denotes TRAFs
T1930 3106-3110 RB denotes also
T1931 3111-3119 VB denotes function
T1932 3120-3122 IN denotes as
T1933 3123-3125 NN denotes E3
T1934 3126-3135 NN denotes ubiquitin
T1935 3136-3143 NN denotes ligases
T1936 3143-3144 -COMMA- denotes ,
T1937 3145-3148 CC denotes and
T1938 3149-3153 DT denotes this
T1939 3154-3163 JJ denotes enzymatic
T1940 3164-3172 NN denotes activity
T1941 3173-3176 MD denotes may
T1942 3177-3187 VB denotes contribute
T1943 3188-3190 TO denotes to
T1944 3191-3197 NN denotes signal
T1945 3198-3209 NN denotes propagation
T1946 3210-3213 CC denotes and
T1947 3214-3224 NN denotes regulation
T1948 3226-3231 IN denotes Among
T1949 3232-3235 DT denotes the
T1950 3236-3244 JJ denotes multiple
T1951 3245-3250 NN denotes TRAFs
T1952 3251-3255 WDT denotes that
T1953 3256-3265 VB denotes associate
T1954 3266-3270 IN denotes with
T1955 3271-3275 NN denotes CD40
T1956 3275-3276 -COMMA- denotes ,
T1957 3277-3282 NN denotes TRAF3
T1958 3283-3286 MD denotes can
T1959 3287-3295 VB denotes function
T1960 3296-3298 IN denotes as
T1961 3299-3300 DT denotes a
T1962 3301-3309 JJ denotes negative
T1963 3310-3319 NN denotes regulator
T1964 3320-3322 IN denotes of
T1965 3323-3332 NN denotes signaling
T1966 3332-3333 -COMMA- denotes ,
T1967 3334-3339 IN denotes while
T1968 3340-3345 NN denotes TRAF2
T1969 3346-3349 CC denotes and
T1970 3350-3355 NN denotes TRAF6
T1971 3356-3363 VB denotes promote
T1972 3364-3367 DT denotes the
T1973 3368-3378 NN denotes activation
T1974 3379-3381 IN denotes of
T1975 3382-3392 JJ denotes downstream
T1976 3393-3402 NN denotes signaling
T1977 3403-3411 NN denotes pathways
T1978 3412-3413 -LRB- denotes [
T1979 3413-3414 CD denotes 4
T1980 3414-3415 -RRB- denotes ]
T1981 3417-3419 PRP denotes We
T1982 3420-3428 RB denotes recently
T1983 3429-3441 VB denotes demonstrated
T1984 3442-3446 IN denotes that
T1985 3447-3454 NN denotes HOIL-1L
T1986 3455-3466 VB denotes interacting
T1987 3467-3474 NN denotes protein
T1988 3475-3476 -LRB- denotes (
T1989 3476-3480 NN denotes HOIP
T1990 3480-3481 -RRB- denotes )
T1991 3481-3482 -COMMA- denotes ,
T1992 3483-3484 DT denotes a
T1993 3485-3494 NN denotes ubiquitin
T1994 3495-3501 NN denotes ligase
T1995 3502-3506 WDT denotes that
T1996 3507-3510 MD denotes can
T1997 3511-3519 VB denotes catalyze
T1998 3520-3523 DT denotes the
T1999 3524-3532 NN denotes assembly
T2000 3533-3535 IN denotes of
T2001 3536-3542 JJ denotes linear
T2002 3543-3556 NN denotes polyubiquitin
T2003 3557-3563 NN denotes chains
T2004 3564-3565 -LRB- denotes [
T2005 3565-3566 CD denotes 5
T2006 3566-3567 -RRB- denotes ]
T2007 3567-3568 -COMMA- denotes ,
T2008 3569-3571 VB denotes is
T2009 3572-3581 VB denotes recruited
T2010 3582-3584 TO denotes to
T2011 3585-3589 NN denotes CD40
T2012 3590-3592 IN denotes in
T2013 3593-3594 DT denotes a
T2014 3595-3610 JJ denotes TRAF2-dependent
T2015 3611-3617 NN denotes manner
T2016 3618-3627 VB denotes following
T2017 3628-3638 NN denotes engagement
T2018 3639-3641 IN denotes of
T2019 3642-3646 NN denotes CD40
T2020 3647-3649 IN denotes by
T2021 3650-3659 JJ denotes agonistic
T2022 3660-3668 NN denotes antibody
T2023 3669-3670 -LRB- denotes [
T2024 3670-3671 CD denotes 6
T2025 3671-3672 -RRB- denotes ]
T2026 3674-3679 DT denotes These
T2027 3680-3683 CC denotes and
T2028 3684-3689 JJ denotes other
T2029 3690-3698 NN denotes findings
T2030 3699-3702 VB denotes led
T2031 3703-3705 PRP denotes us
T2032 3706-3708 TO denotes to
T2033 3709-3720 VB denotes hypothesize
T2034 3721-3725 IN denotes that
T2035 3726-3730 NN denotes HOIP
T2036 3731-3740 VB denotes functions
T2037 3741-3751 RB denotes downstream
T2038 3752-3754 IN denotes of
T2039 3755-3760 NN denotes TRAF2
T2040 3761-3763 IN denotes in
T2041 3764-3767 DT denotes the
T2042 3768-3772 NN denotes CD40
T2043 3773-3782 NN denotes signaling
T2044 3783-3790 NN denotes pathway
T2045 3791-3794 CC denotes and
T2046 3795-3799 IN denotes that
T2047 3800-3804 NN denotes HOIP
T7475 3800-3804 NN denotes HOIP
T2048 3805-3807 VB denotes is
T7476 3805-3807 VB denotes is
T2049 3808-3817 JJ denotes necessary
T7477 3808-3817 JJ denotes necessary
T2050 3818-3821 IN denotes for
T7478 3818-3821 IN denotes for
T2051 3822-3825 DT denotes the
T2052 3826-3836 NN denotes activation
T2053 3837-3839 IN denotes of
T2054 3840-3845 NN denotes NF-κB
T2055 3846-3849 CC denotes and
T2056 3850-3858 RB denotes possibly
T2057 3859-3864 JJ denotes other
T2058 3865-3874 VB denotes signaling
T2059 3875-3884 NN denotes molecules
T2060 3886-3888 TO denotes To
T2061 3889-3893 VB denotes test
T2062 3894-3898 DT denotes this
T2063 3899-3909 NN denotes hypothesis
T2064 3909-3910 -COMMA- denotes ,
T2065 3911-3913 PRP denotes we
T2066 3914-3922 VB denotes employed
T2067 3923-3930 JJ denotes somatic
T2068 3931-3935 NN denotes cell
T2069 3936-3940 NN denotes gene
T2070 3941-3950 VB denotes targeting
T2071 3951-3953 TO denotes to
T2072 3954-3960 VB denotes ablate
T2073 3961-3971 NN denotes expression
T2074 3972-3974 IN denotes of
T2075 3975-3979 NN denotes HOIP
T2076 3980-3982 IN denotes in
T2077 3983-3984 DT denotes a
T2078 3985-3990 NN denotes mouse
T2079 3991-3992 NN denotes B
T2080 3993-3997 NN denotes cell
T2081 3998-4002 NN denotes line
T2082 4003-4007 WDT denotes that
T2083 4008-4011 VB denotes has
T2084 4012-4018 VB denotes proven
T2085 4019-4021 TO denotes to
T2086 4022-4024 VB denotes be
T2087 4025-4026 DT denotes a
T2088 4027-4033 JJ denotes useful
T2089 4034-4039 NN denotes model
T2090 4040-4043 IN denotes for
T2091 4044-4045 NN denotes B
T2092 4046-4050 NN denotes cell
T2093 4051-4055 NN denotes CD40
T2094 4056-4065 NN denotes signaling
T2095 4066-4067 -LRB- denotes [
T2096 4067-4068 CD denotes 7
T2097 4068-4069 -RRB- denotes ]
T2098 4069-4070 -COMMA- denotes ,
T2099 4071-4072 -LRB- denotes [
T2100 4072-4073 CD denotes 8
T2101 4073-4074 -RRB- denotes ]
T2102 4074-4075 -COMMA- denotes ,
T2103 4076-4077 -LRB- denotes [
T2104 4077-4078 CD denotes 9
T2105 4078-4079 -RRB- denotes ]
T2106 4081-4083 PRP denotes We
T2107 4084-4089 VB denotes found
T2108 4090-4094 IN denotes that
T2109 4095-4098 DT denotes the
T2110 4099-4111 JJ denotes CD40-induced
T2111 4112-4124 NN denotes upregulation
T2112 4125-4127 IN denotes of
T2113 4128-4132 NN denotes CD80
T2114 4133-4134 -LRB- denotes (
T2115 4134-4135 DT denotes a
T2116 4136-4149 JJ denotes costimulatory
T2117 4150-4158 NN denotes molecule
T2118 4159-4162 IN denotes for
T2119 4163-4164 NN denotes T
T2120 4165-4170 NN denotes cells
T2121 4170-4171 -RRB- denotes )
T2122 4172-4175 VB denotes was
T2123 4176-4185 JJ denotes defective
T2124 4186-4188 IN denotes in
T2125 4189-4203 JJ denotes HOIP-deficient
T2126 4204-4209 NN denotes cells
T2127 4211-4220 RB denotes Similarly
T2128 4220-4221 -COMMA- denotes ,
T2129 4222-4225 DT denotes the
T2130 4226-4230 NN denotes CD40
T2131 4231-4234 CC denotes and
T2132 4235-4239 NN denotes IL-4
T2133 4240-4246 JJ denotes driven
T2134 4247-4257 NN denotes production
T2135 4258-4260 IN denotes of
T2136 4261-4269 NN denotes germline
T2137 4270-4281 NN denotes transcripts
T2138 4282-4286 IN denotes from
T2139 4287-4290 DT denotes the
T2140 4291-4305 NN denotes immunoglobulin
T2141 4306-4313 NN denotes epsilon
T2142 4314-4319 JJ denotes heavy
T2143 4320-4325 NN denotes chain
T2144 4326-4331 NN denotes locus
T2145 4331-4332 -COMMA- denotes ,
T2146 4333-4335 DT denotes an
T2147 4336-4341 NN denotes event
T2148 4342-4346 WDT denotes that
T2149 4347-4355 VB denotes precedes
T2150 4356-4370 NN denotes immunoglobulin
T2151 4371-4375 NN denotes gene
T2152 4376-4389 NN denotes rearrangement
T2153 4390-4393 CC denotes and
T2154 4394-4401 NN denotes isotype
T2155 4402-4411 NN denotes switching
T2156 4411-4412 -COMMA- denotes ,
T2157 4413-4416 VB denotes was
T2158 4417-4426 JJ denotes defective
T2159 4427-4429 IN denotes in
T2160 4430-4433 DT denotes the
T2161 4434-4441 NN denotes absence
T2162 4442-4444 IN denotes of
T2163 4445-4449 NN denotes HOIP
T2164 4451-4453 PRP denotes We
T2165 4454-4458 RB denotes also
T2166 4459-4464 VB denotes found
T2167 4465-4469 IN denotes that
T2168 4470-4473 DT denotes the
T2169 4474-4487 JJ denotes CD40-mediated
T2170 4488-4498 NN denotes activation
T2171 4499-4501 IN denotes of
T2172 4502-4507 NN denotes NF-κB
T2173 4508-4511 CC denotes and
T2174 4512-4515 DT denotes the
T2175 4516-4532 JJ denotes stress-activated
T2176 4533-4540 NN denotes protein
T2177 4541-4547 NN denotes kinase
T2178 4548-4553 NN denotes c-Jun
T2179 4554-4560 NN denotes kinase
T2180 4561-4562 -LRB- denotes (
T2181 4562-4565 NN denotes JNK
T2182 4565-4566 -RRB- denotes )
T2183 4567-4570 VB denotes was
T2184 4571-4580 JJ denotes defective
T2185 4581-4583 IN denotes in
T2186 4584-4598 JJ denotes HOIP-deficient
T2187 4599-4604 NN denotes cells
T2188 4606-4616 JJ denotes Consistent
T2189 4617-4621 IN denotes with
T2190 4622-4630 JJ denotes impaired
T2191 4631-4636 NN denotes NF-κB
T2192 4637-4647 NN denotes activation
T2193 4647-4648 -COMMA- denotes ,
T2194 4649-4660 NN denotes association
T7479 4649-4660 NN denotes association
T2195 4661-4663 IN denotes of
T7480 4661-4663 IN denotes of
T2196 4664-4667 DT denotes the
T7481 4664-4667 DT denotes the
T2197 4668-4671 NN denotes IκB
T7482 4668-13422 NN denotes IκB kinase (IKK) complex with CD40 was undetectable in HOIP-deficient cells. Together, our results indicate that HOIP plays a critical role in the activation of signaling pathways that regulate cellular responses to CD40 engagement. Results Generation of HOIP-deficient B cells via targeted disruption of Rnf31, the gene encoding HOIP In our previous study, we demonstrated that HOIP is recruited to the CD40 signaling complex in two mouse B cell lines [6]. Similar results were obtained with mouse splenocytes, indicating that the interaction of CD40 with HOIP is not limited to transformed B cell lines (unpublished observations). We also found that the recruitment of HOIP to CD40 was TRAF2-dependent and that overexpression of a truncated HOIP mutant partially inhibited CD40-mediated NF-κB activation. These results support the hypothesis that HOIP plays a role in CD40 signal transduction. To further define the role and evaluate the importance of HOIP in CD40 signaling, we used somatic cell gene targeting to disrupt the gene encoding HOIP in the mouse B cell line A20.2J. This cell line has been particularly useful in the characterization of CD40 signaling mechanisms due to the relative ease with which its genome can be modified by homologous recombination [7], [8], [9]. We used a targeting vector capable of undergoing homologous recombination with Rnf31 (the gene encoding HOIP) to disrupt the coding sequence of the gene in exon 5 (Fig. 1A). Following introduction of the vector, the neomycin-resistant clones that arose were screened by PCR amplification of genomic DNA to identify cells containing a disrupted Rnf31 allele. To remove the selectable marker gene cassette from the disrupted Rnf31 allele, recombinant cell lines were transiently transfected with a plasmid that encodes Cre recombinase. This step allowed us to perform a second round of targeting and drug selection, generating cells in which both copies of Rnf31 were disrupted. Two independent clonal cell lines were chosen for further analysis. HOIP protein expression was undetectable in both cell lines, as determined by Western blot analysis of cell lysates (Fig. 1B), demonstrating that the targeting process was successful. In the text that follows, the two gene-targeted cell lines are referred to as HOIP-deficient cells. 10.1371/journal.pone.0023061.g001 Figure 1 HOIP gene targeting. (A) Regions of Rnf31 gene sequence (bottom) used in the HOIP gene targeting vector (top) are shown. Arrows (F and R) indicate approximate positions of sequences homologous to oligonucleotides used for PCR-mediated detection of homologous recombination. Homologous recombination of the vector with Rnf31 resulted in a small chromosomal deletion and the in-frame insertion of neomycin phosphotransferase (NeoR) into the amino-terminal HOIP coding sequence. A diphtheria toxin (DT) cassette in the vector facilitates the negative selection of cells in which random chromosomal insertion of the vector takes place. LoxP sequences allow the Cre-mediated deletion of the NeoR coding sequence. The SV40pA sequence helps to ensure disruption of gene expression after deletion of the NeoR sequence. (B) Anti-HOIP and anti-FLAG Western blots of cell lysates from A20.2J cells and HOIP-deficient (HOIP-/-) clones. A partial decrease in HOIP protein expression is evident in cells following disruption of one copy of Rnf31 (HOIP−/+). HOIP expression in clones reconstituted with an empty retroviral vector (pMIP) or a retroviral vector encoding FLAG-tagged HOIP is also shown. Approximate molecular weight of HOIP is 120 kD. To enable us to confirm that any signaling or functional defects observed in HOIP-deficient cells were due to disruption of the Rnf31 gene, both HOIP-deficient cell lines were transduced with a retrovirus encoding FLAG-tagged wild-type HOIP, thus restoring HOIP protein expression (Fig. 1B). These cells are referred to as HOIP-reconstituted cells. In the experiments that follow, responses by these cells were compared to those of A20.2J cells and HOIP-deficient cells transduced with a retroviral vector (pMIP) lacking a cDNA insert (empty vector). HOIP is required for CD40-mediated CD80 upregulation and activation of germline epsilon transcription Engagement of CD40 on B cells upregulates expression of CD80 [10], a cell surface protein that promotes activation of T cells interacting with B cells and other APC. To begin evaluating potential contributions of HOIP to CD40 signaling, we assayed the upregulation of CD80 induced by engagement of CD40 on parental A20.2J, HOIP-deficient, and HOIP-reconstituted cells (Fig. 2). Compared to the parental cell line, the CD40-stimulated upregulation of CD80 was dramatically reduced in all HOIP-deficient cells tested. In contrast, upregulation of CD80 was restored in HOIP-reconstituted cells, confirming that the defect in CD80 upregulation in the HOIP-deficient cells was due to the disruption of Rnf31. 10.1371/journal.pone.0023061.g002 Figure 2 CD40-mediated CD80 upregulation is defective in HOIP-deficient cells. Cells were incubated for 72 hrs with an isotype control antibody (left) or anti-CD40 antibody (right) and then stained for expression of CD80 (filled profiles indicate staining with anti-CD80 antibody; open profiles are staining with an isotype control antibody). Transduction of both HOIP-deficient (HOIP-/-) clones with a retrovirus encoding FLAG-HOIP reversed defective CD80 upregulation, while transduction with an empty vector (pMIP) did not. Similar results were obtained in a second experiment. CD40 signals in B cells can also contribute to the activation of DNA recombination in the immunoglobulin heavy chain locus [11], [12]. This process, known as immunoglobulin class switching (or isotype switching), allows B cells, initially expressing IgM, to switch to IgA, IgG, or IgE depending on the class of antibody most appropriate for a particular immune response. Switching to IgE can be induced by the combination of IL-4 and CD40 signals. The gene rearrangement necessary for IgE production is preceded by the production of non-coding RNA transcripts from germline sequences in the IgE heavy chain locus [13]. To determine whether HOIP is required in the initiation of the class switching process, the relevant B cell lines were stimulated through CD40 in the presence of IL-4 and tested for production of germline epsilon (GLε) transcripts (Fig. 3). Anti-CD40 antibody stimulation alone or together with IL-4 induced GLε transcription in parental A20.2J cells as expected. In contrast, only low levels of GLε expression were detected in HOIP-deficient cells stimulated with either anti-CD40 antibody alone or anti-CD40 and IL-4. Normal levels of GLε expression were induced in HOIP-reconstituted cells stimulated with anti-CD40 antibody alone or together with IL-4. Collectively, these data demonstrate that HOIP has a critical role in cellular responses to CD40 signaling. 10.1371/journal.pone.0023061.g003 Figure 3 GLε transcription is defective in CD40-stimulated HOIP-deficient cells. A20.2J and HOIP-deficient cells transduced with an empty retroviral vector (A20.2J + pMIP and HOIP-/- + pMIP, respectively), or HOIP-deficient cells transduced with a HOIP-encoding retrovirus (HOIP-/- + HOIP) were cultured overnight with agonistic anti-CD40 or an isotype control antibody (iso), with or without IL-4. RNA was isolated from the cells, reverse-transcribed, and then subjected to quantitative PCR to determine levels of GLε transcripts. Results were normalized to the levels of Hprt1 transcripts in each sample. Symbols indicate the values from duplicate cultures (a line indicates the mean of the two values). Similar results were obtained in a second experiment and in an additional experiment with a second HOIP-deficient clone. HOIP mediates CD40-stimulated NF-κB and JNK activation The results described above show that HOIP plays an important role in CD40-mediated effector functions of B cells. It follows that HOIP is likely a key mediator of CD40 signaling. To test this hypothesis, we stimulated A20.2J and HOIP-deficient cells with CD154 (CD40 ligand) expressed by HI5 insect cells [7], [8] and measured activation of the NF-κB and JNK pathways, two of the major transcriptional regulators activated by CD40 [4]. Cell-associated CD154 was used as the stimulus in these experiments as it typically provides more robust, and therefore more readily detected, activation signals than does anti-CD40 antibody. Activation of the canonical NF-κB pathway is initiated with the phosphorylation of IκB proteins by the IκB kinase complex (IKK). In resting cells, IκB proteins are responsible for sequestering NF-κB subunits in the cytoplasm. Phosphorylation by the IKK
T2198 4672-4678 NN denotes kinase
T2199 4679-4680 -LRB- denotes (
T2200 4680-4683 NN denotes IKK
T2201 4683-4684 -RRB- denotes )
T2202 4685-4692 NN denotes complex
T2203 4693-4697 IN denotes with
T2204 4698-4702 NN denotes CD40
T2205 4703-4706 VB denotes was
T2206 4707-4719 JJ denotes undetectable
T2207 4720-4722 IN denotes in
T2208 4723-4737 JJ denotes HOIP-deficient
T2209 4738-4743 NN denotes cells
T2210 4745-4753 RB denotes Together
T2211 4753-4754 -COMMA- denotes ,
T2212 4755-4758 PRP-DOLLAR- denotes our
T2213 4759-4766 NN denotes results
T2214 4767-4775 VB denotes indicate
T2215 4776-4780 IN denotes that
T2216 4781-4785 NN denotes HOIP
T2217 4786-4791 VB denotes plays
T2218 4792-4793 DT denotes a
T2219 4794-4802 JJ denotes critical
T2220 4803-4807 NN denotes role
T2221 4808-4810 IN denotes in
T2222 4811-4814 DT denotes the
T2223 4815-4825 NN denotes activation
T2224 4826-4828 IN denotes of
T2225 4829-4838 NN denotes signaling
T2226 4839-4847 NN denotes pathways
T2227 4848-4852 WDT denotes that
T2228 4853-4861 VB denotes regulate
T2229 4862-4870 JJ denotes cellular
T2230 4871-4880 NN denotes responses
T2231 4881-4883 TO denotes to
T2232 4884-4888 NN denotes CD40
T2233 4889-4899 NN denotes engagement
T3341 4911-4921 NN denotes Generation
T3342 4922-4924 IN denotes of
T3343 4925-4939 JJ denotes HOIP-deficient
T3344 4940-4941 NN denotes B
T3345 4942-4947 NN denotes cells
T3346 4948-4951 IN denotes via
T3347 4952-4960 VB denotes targeted
T3348 4961-4971 NN denotes disruption
T3349 4972-4974 IN denotes of
T3350 4975-4980 NN denotes Rnf31
T3351 4980-4981 -COMMA- denotes ,
T3352 4982-4985 DT denotes the
T3353 4986-4990 NN denotes gene
T3354 4991-4999 VB denotes encoding
T3355 5000-5004 NN denotes HOIP
T3356 5005-5007 IN denotes In
T3357 5008-5011 PRP-DOLLAR- denotes our
T3358 5012-5020 JJ denotes previous
T3359 5021-5026 NN denotes study
T3360 5026-5027 -COMMA- denotes ,
T3361 5028-5030 PRP denotes we
T3362 5031-5043 VB denotes demonstrated
T3363 5044-5048 IN denotes that
T3364 5049-5053 NN denotes HOIP
T3365 5054-5056 VB denotes is
T3366 5057-5066 VB denotes recruited
T3367 5067-5069 TO denotes to
T3368 5070-5073 DT denotes the
T3369 5074-5078 NN denotes CD40
T3370 5079-5088 NN denotes signaling
T3371 5089-5096 NN denotes complex
T3372 5097-5099 IN denotes in
T3373 5100-5103 CD denotes two
T3374 5104-5109 NN denotes mouse
T3375 5110-5111 NN denotes B
T3376 5112-5116 NN denotes cell
T3377 5117-5122 NN denotes lines
T3378 5123-5124 -LRB- denotes [
T3379 5124-5125 CD denotes 6
T3380 5125-5126 -RRB- denotes ]
T3381 5128-5135 JJ denotes Similar
T3382 5136-5143 NN denotes results
T3383 5144-5148 VB denotes were
T3384 5149-5157 VB denotes obtained
T3385 5158-5162 IN denotes with
T3386 5163-5168 NN denotes mouse
T3387 5169-5180 NN denotes splenocytes
T3388 5180-5181 -COMMA- denotes ,
T3389 5182-5192 VB denotes indicating
T3390 5193-5197 IN denotes that
T3391 5198-5201 DT denotes the
T3392 5202-5213 NN denotes interaction
T3393 5214-5216 IN denotes of
T3394 5217-5221 NN denotes CD40
T3395 5222-5226 IN denotes with
T3396 5227-5231 NN denotes HOIP
T3397 5232-5234 VB denotes is
T3398 5235-5238 RB denotes not
T3399 5239-5246 VB denotes limited
T3400 5247-5249 TO denotes to
T3401 5250-5261 VB denotes transformed
T3402 5262-5263 NN denotes B
T3403 5264-5268 NN denotes cell
T3404 5269-5274 NN denotes lines
T3405 5275-5276 -LRB- denotes (
T3406 5276-5287 JJ denotes unpublished
T3407 5288-5300 NN denotes observations
T3408 5300-5301 -RRB- denotes )
T3409 5303-5305 PRP denotes We
T3410 5306-5310 RB denotes also
T3411 5311-5316 VB denotes found
T3412 5317-5321 IN denotes that
T3413 5322-5325 DT denotes the
T3414 5326-5337 NN denotes recruitment
T3415 5338-5340 IN denotes of
T3416 5341-5345 NN denotes HOIP
T3417 5346-5348 TO denotes to
T3418 5349-5353 NN denotes CD40
T3419 5354-5357 VB denotes was
T3420 5358-5373 JJ denotes TRAF2-dependent
T3421 5374-5377 CC denotes and
T3422 5378-5382 IN denotes that
T3423 5383-5397 NN denotes overexpression
T3424 5398-5400 IN denotes of
T3425 5401-5402 DT denotes a
T3426 5403-5412 VB denotes truncated
T3427 5413-5417 NN denotes HOIP
T3428 5418-5424 NN denotes mutant
T3429 5425-5434 RB denotes partially
T3430 5435-5444 VB denotes inhibited
T3431 5445-5458 JJ denotes CD40-mediated
T3432 5459-5464 NN denotes NF-κB
T3433 5465-5475 NN denotes activation
T3434 5477-5482 DT denotes These
T3435 5483-5490 NN denotes results
T3436 5491-5498 VB denotes support
T3437 5499-5502 DT denotes the
T3438 5503-5513 NN denotes hypothesis
T3439 5514-5518 IN denotes that
T3440 5519-5523 NN denotes HOIP
T3441 5524-5529 VB denotes plays
T3442 5530-5531 DT denotes a
T3443 5532-5536 NN denotes role
T3444 5537-5539 IN denotes in
T3445 5540-5544 NN denotes CD40
T3446 5545-5551 NN denotes signal
T3447 5552-5564 NN denotes transduction
T3448 5566-5568 TO denotes To
T3449 5569-5576 RB denotes further
T3450 5577-5583 VB denotes define
T3451 5584-5587 DT denotes the
T3452 5588-5592 NN denotes role
T3453 5593-5596 CC denotes and
T3454 5597-5605 VB denotes evaluate
T3455 5606-5609 DT denotes the
T3456 5610-5620 NN denotes importance
T3457 5621-5623 IN denotes of
T3458 5624-5628 NN denotes HOIP
T3459 5629-5631 IN denotes in
T3460 5632-5636 NN denotes CD40
T3461 5637-5646 NN denotes signaling
T3462 5646-5647 -COMMA- denotes ,
T3463 5648-5650 PRP denotes we
T3464 5651-5655 VB denotes used
T3465 5656-5663 JJ denotes somatic
T3466 5664-5668 NN denotes cell
T3467 5669-5673 NN denotes gene
T3468 5674-5683 VB denotes targeting
T3469 5684-5686 TO denotes to
T3470 5687-5694 VB denotes disrupt
T3471 5695-5698 DT denotes the
T3472 5699-5703 NN denotes gene
T3473 5704-5712 VB denotes encoding
T3474 5713-5717 NN denotes HOIP
T3475 5718-5720 IN denotes in
T3476 5721-5724 DT denotes the
T3477 5725-5730 NN denotes mouse
T3478 5731-5732 NN denotes B
T3479 5733-5737 NN denotes cell
T3480 5738-5742 NN denotes line
T3481 5743-5749 NN denotes A20.2J
T3482 5751-5755 DT denotes This
T3483 5756-5760 NN denotes cell
T3484 5761-5765 NN denotes line
T3485 5766-5769 VB denotes has
T3486 5770-5774 VB denotes been
T3487 5775-5787 RB denotes particularly
T3488 5788-5794 JJ denotes useful
T3489 5795-5797 IN denotes in
T3490 5798-5801 DT denotes the
T3491 5802-5818 NN denotes characterization
T3492 5819-5821 IN denotes of
T3493 5822-5826 NN denotes CD40
T3494 5827-5836 NN denotes signaling
T3495 5837-5847 NN denotes mechanisms
T3496 5848-5851 JJ denotes due
T3497 5852-5854 TO denotes to
T3498 5855-5858 DT denotes the
T3499 5859-5867 JJ denotes relative
T3500 5868-5872 NN denotes ease
T3501 5873-5877 IN denotes with
T3502 5878-5883 WDT denotes which
T3503 5884-5887 PRP-DOLLAR- denotes its
T3504 5888-5894 NN denotes genome
T3505 5895-5898 MD denotes can
T3506 5899-5901 VB denotes be
T3507 5902-5910 VB denotes modified
T3508 5911-5913 IN denotes by
T3509 5914-5924 JJ denotes homologous
T3510 5925-5938 NN denotes recombination
T3511 5939-5940 -LRB- denotes [
T3512 5940-5941 CD denotes 7
T3513 5941-5942 -RRB- denotes ]
T3514 5942-5943 -COMMA- denotes ,
T3515 5944-5945 -LRB- denotes [
T3516 5945-5946 CD denotes 8
T3517 5946-5947 -RRB- denotes ]
T3518 5947-5948 -COMMA- denotes ,
T3519 5949-5950 -LRB- denotes [
T3520 5950-5951 CD denotes 9
T3521 5951-5952 -RRB- denotes ]
T3522 5954-5956 PRP denotes We
T3523 5957-5961 VB denotes used
T3524 5962-5963 DT denotes a
T3525 5964-5973 VB denotes targeting
T3526 5974-5980 NN denotes vector
T3527 5981-5988 JJ denotes capable
T3528 5989-5991 IN denotes of
T3529 5992-6002 VB denotes undergoing
T3530 6003-6013 JJ denotes homologous
T3531 6014-6027 NN denotes recombination
T3532 6028-6032 IN denotes with
T3533 6033-6038 NN denotes Rnf31
T3534 6039-6040 -LRB- denotes (
T3535 6040-6043 DT denotes the
T3536 6044-6048 NN denotes gene
T3537 6049-6057 VB denotes encoding
T3538 6058-6062 NN denotes HOIP
T3539 6062-6063 -RRB- denotes )
T3540 6064-6066 TO denotes to
T3541 6067-6074 VB denotes disrupt
T3542 6075-6078 DT denotes the
T3543 6079-6085 VB denotes coding
T3544 6086-6094 NN denotes sequence
T3545 6095-6097 IN denotes of
T3546 6098-6101 DT denotes the
T3547 6102-6106 NN denotes gene
T3548 6107-6109 IN denotes in
T3549 6110-6114 NN denotes exon
T3550 6115-6116 CD denotes 5
T3551 6117-6118 -LRB- denotes (
T3552 6118-6122 NNP denotes Fig.
T3553 6123-6125 NN denotes 1A
T3554 6125-6126 -RRB- denotes )
T3555 6128-6137 VB denotes Following
T3556 6138-6150 NN denotes introduction
T3557 6151-6153 IN denotes of
T3558 6154-6157 DT denotes the
T3559 6158-6164 NN denotes vector
T3560 6164-6165 -COMMA- denotes ,
T3561 6166-6169 DT denotes the
T3562 6170-6188 JJ denotes neomycin-resistant
T3563 6189-6195 NN denotes clones
T3564 6196-6200 WDT denotes that
T3565 6201-6206 VB denotes arose
T3566 6207-6211 VB denotes were
T3567 6212-6220 VB denotes screened
T3568 6221-6223 IN denotes by
T3569 6224-6227 NN denotes PCR
T3570 6228-6241 NN denotes amplification
T3571 6242-6244 IN denotes of
T3572 6245-6252 JJ denotes genomic
T3573 6253-6256 NN denotes DNA
T3574 6257-6259 TO denotes to
T3575 6260-6268 VB denotes identify
T3576 6269-6274 NN denotes cells
T3577 6275-6285 VB denotes containing
T3578 6286-6287 DT denotes a
T3579 6288-6297 VB denotes disrupted
T3580 6298-6303 NN denotes Rnf31
T3581 6304-6310 NN denotes allele
T3582 6312-6314 TO denotes To
T3583 6315-6321 VB denotes remove
T3584 6322-6325 DT denotes the
T3585 6326-6336 JJ denotes selectable
T3586 6337-6343 NN denotes marker
T3587 6344-6348 NN denotes gene
T3588 6349-6357 NN denotes cassette
T3589 6358-6362 IN denotes from
T3590 6363-6366 DT denotes the
T3591 6367-6376 VB denotes disrupted
T3592 6377-6382 NN denotes Rnf31
T3593 6383-6389 NN denotes allele
T3594 6389-6390 -COMMA- denotes ,
T3595 6391-6402 JJ denotes recombinant
T3596 6403-6407 NN denotes cell
T3597 6408-6413 NN denotes lines
T3598 6414-6418 VB denotes were
T3599 6419-6430 RB denotes transiently
T3600 6431-6442 VB denotes transfected
T3601 6443-6447 IN denotes with
T3602 6448-6449 DT denotes a
T3603 6450-6457 NN denotes plasmid
T3604 6458-6462 WDT denotes that
T3605 6463-6470 VB denotes encodes
T3606 6471-6474 NNP denotes Cre
T3607 6475-6486 NN denotes recombinase
T3608 6488-6492 DT denotes This
T3609 6493-6497 NN denotes step
T3610 6498-6505 VB denotes allowed
T3611 6506-6508 PRP denotes us
T3612 6509-6511 TO denotes to
T3613 6512-6519 VB denotes perform
T3614 6520-6521 DT denotes a
T3615 6522-6528 JJ denotes second
T3616 6529-6534 NN denotes round
T3617 6535-6537 IN denotes of
T3618 6538-6547 VB denotes targeting
T3619 6548-6551 CC denotes and
T3620 6552-6556 NN denotes drug
T3621 6557-6566 NN denotes selection
T3622 6566-6567 -COMMA- denotes ,
T3623 6568-6578 VB denotes generating
T3624 6579-6584 NN denotes cells
T3625 6585-6587 IN denotes in
T3626 6588-6593 WDT denotes which
T3627 6594-6598 DT denotes both
T3628 6599-6605 NN denotes copies
T3629 6606-6608 IN denotes of
T3630 6609-6614 NN denotes Rnf31
T3631 6615-6619 VB denotes were
T3632 6620-6629 VB denotes disrupted
T3633 6631-6634 CD denotes Two
T3634 6635-6646 JJ denotes independent
T3635 6647-6653 JJ denotes clonal
T3636 6654-6658 NN denotes cell
T3637 6659-6664 NN denotes lines
T3638 6665-6669 VB denotes were
T3639 6670-6676 VB denotes chosen
T3640 6677-6680 IN denotes for
T3641 6681-6688 JJ denotes further
T3642 6689-6697 NN denotes analysis
T3643 6699-6703 NN denotes HOIP
T3644 6704-6711 NN denotes protein
T3645 6712-6722 NN denotes expression
T3646 6723-6726 VB denotes was
T3647 6727-6739 JJ denotes undetectable
T3648 6740-6742 IN denotes in
T3649 6743-6747 DT denotes both
T3650 6748-6752 NN denotes cell
T3651 6753-6758 NN denotes lines
T3652 6758-6759 -COMMA- denotes ,
T3653 6760-6762 IN denotes as
T3654 6763-6773 VB denotes determined
T3655 6774-6776 IN denotes by
T3656 6777-6784 JJ denotes Western
T3657 6785-6789 NN denotes blot
T3658 6790-6798 NN denotes analysis
T3659 6799-6801 IN denotes of
T3660 6802-6806 NN denotes cell
T3661 6807-6814 NN denotes lysates
T3662 6815-6816 -LRB- denotes (
T3663 6816-6820 NNP denotes Fig.
T3664 6821-6823 NN denotes 1B
T3665 6823-6824 -RRB- denotes )
T3666 6824-6825 -COMMA- denotes ,
T3667 6826-6839 VB denotes demonstrating
T3668 6840-6844 IN denotes that
T3669 6845-6848 DT denotes the
T3670 6849-6858 VB denotes targeting
T3671 6859-6866 NN denotes process
T3672 6867-6870 VB denotes was
T3673 6871-6881 JJ denotes successful
T3674 6883-6885 IN denotes In
T3675 6886-6889 DT denotes the
T3676 6890-6894 NN denotes text
T3677 6895-6899 WDT denotes that
T3678 6900-6907 VB denotes follows
T3679 6907-6908 -COMMA- denotes ,
T3680 6909-6912 DT denotes the
T3681 6913-6916 CD denotes two
T3682 6917-6930 JJ denotes gene-targeted
T3683 6931-6935 NN denotes cell
T3684 6936-6941 NN denotes lines
T3685 6942-6945 VB denotes are
T3686 6946-6954 VB denotes referred
T3687 6955-6957 TO denotes to
T3688 6958-6960 IN denotes as
T3689 6961-6975 JJ denotes HOIP-deficient
T3690 6976-6981 NN denotes cells
T15027 7027-7031 NN denotes HOIP
T15028 7032-7036 NN denotes gene
T15029 7037-7046 NN denotes targeting
T15030 7048-7049 -LRB- denotes (
T15031 7049-7050 NN denotes A
T15032 7050-7051 -RRB- denotes )
T15033 7052-7059 NN denotes Regions
T15034 7060-7062 IN denotes of
T15035 7063-7068 NN denotes Rnf31
T15036 7069-7073 NN denotes gene
T15037 7074-7082 NN denotes sequence
T15038 7083-7084 -LRB- denotes (
T15039 7084-7090 NN denotes bottom
T15040 7090-7091 -RRB- denotes )
T15041 7092-7096 VB denotes used
T15042 7097-7099 IN denotes in
T15043 7100-7103 DT denotes the
T15044 7104-7108 NN denotes HOIP
T15045 7109-7113 NN denotes gene
T15046 7114-7123 NN denotes targeting
T15047 7124-7130 NN denotes vector
T15048 7131-7132 -LRB- denotes (
T15049 7132-7135 NN denotes top
T15050 7135-7136 -RRB- denotes )
T15051 7137-7140 VB denotes are
T15052 7141-7146 VB denotes shown
T15053 7148-7154 NN denotes Arrows
T15054 7155-7156 -LRB- denotes (
T15055 7156-7157 NN denotes F
T15056 7158-7161 CC denotes and
T15057 7162-7163 NN denotes R
T15058 7163-7164 -RRB- denotes )
T15059 7165-7173 VB denotes indicate
T15060 7174-7185 JJ denotes approximate
T15061 7186-7195 NN denotes positions
T15062 7196-7198 IN denotes of
T15063 7199-7208 NN denotes sequences
T15064 7209-7219 JJ denotes homologous
T15065 7220-7222 TO denotes to
T15066 7223-7239 NN denotes oligonucleotides
T15067 7240-7244 VB denotes used
T15068 7245-7248 IN denotes for
T15069 7249-7261 JJ denotes PCR-mediated
T15070 7262-7271 NN denotes detection
T15071 7272-7274 IN denotes of
T15072 7275-7285 JJ denotes homologous
T15073 7286-7299 NN denotes recombination
T15074 7301-7311 JJ denotes Homologous
T15075 7312-7325 NN denotes recombination
T15076 7326-7328 IN denotes of
T15077 7329-7332 DT denotes the
T15078 7333-7339 NN denotes vector
T15079 7340-7344 IN denotes with
T15080 7345-7350 NN denotes Rnf31
T15081 7351-7359 VB denotes resulted
T15082 7360-7362 IN denotes in
T15083 7363-7364 DT denotes a
T15084 7365-7370 JJ denotes small
T15085 7371-7382 JJ denotes chromosomal
T15086 7383-7391 NN denotes deletion
T15087 7392-7395 CC denotes and
T15088 7396-7399 DT denotes the
T15089 7400-7408 JJ denotes in-frame
T15090 7409-7418 NN denotes insertion
T15091 7419-7421 IN denotes of
T15092 7422-7430 NN denotes neomycin
T15093 7431-7449 NN denotes phosphotransferase
T15094 7450-7451 -LRB- denotes (
T15095 7451-7455 NN denotes NeoR
T15096 7455-7456 -RRB- denotes )
T15097 7457-7461 IN denotes into
T15098 7462-7465 DT denotes the
T15099 7466-7480 JJ denotes amino-terminal
T15100 7481-7485 NN denotes HOIP
T15101 7486-7492 VB denotes coding
T15102 7493-7501 NN denotes sequence
T15103 7503-7504 DT denotes A
T15104 7505-7515 NN denotes diphtheria
T15105 7516-7521 NN denotes toxin
T15106 7522-7523 -LRB- denotes (
T15107 7523-7525 NN denotes DT
T15108 7525-7526 -RRB- denotes )
T15109 7527-7535 NN denotes cassette
T15110 7536-7538 IN denotes in
T15111 7539-7542 DT denotes the
T15112 7543-7549 NN denotes vector
T15113 7550-7561 VB denotes facilitates
T15114 7562-7565 DT denotes the
T15115 7566-7574 JJ denotes negative
T15116 7575-7584 NN denotes selection
T15117 7585-7587 IN denotes of
T15118 7588-7593 NN denotes cells
T15119 7594-7596 IN denotes in
T15120 7597-7602 WDT denotes which
T15121 7603-7609 VB denotes random
T15122 7610-7621 JJ denotes chromosomal
T15123 7622-7631 NN denotes insertion
T15124 7632-7634 IN denotes of
T15125 7635-7638 DT denotes the
T15126 7639-7645 NN denotes vector
T15127 7646-7651 VB denotes takes
T15128 7652-7657 NN denotes place
T15129 7659-7663 NN denotes LoxP
T15130 7664-7673 NN denotes sequences
T15131 7674-7679 VB denotes allow
T15132 7680-7683 DT denotes the
T15133 7684-7696 JJ denotes Cre-mediated
T15134 7697-7705 NN denotes deletion
T15135 7706-7708 IN denotes of
T15136 7709-7712 DT denotes the
T15137 7713-7717 NN denotes NeoR
T15138 7718-7724 NN denotes coding
T15139 7725-7733 NN denotes sequence
T15140 7735-7738 DT denotes The
T15141 7739-7745 NN denotes SV40pA
T15142 7746-7754 NN denotes sequence
T15143 7755-7760 VB denotes helps
T15144 7761-7763 TO denotes to
T15145 7764-7770 VB denotes ensure
T15146 7771-7781 NN denotes disruption
T15147 7782-7784 IN denotes of
T15148 7785-7789 NN denotes gene
T15149 7790-7800 NN denotes expression
T15150 7801-7806 IN denotes after
T15151 7807-7815 NN denotes deletion
T15152 7816-7818 IN denotes of
T15153 7819-7822 DT denotes the
T15154 7823-7827 NN denotes NeoR
T15155 7828-7837 NN denotes sequence.
T15156 7838-7839 -LRB- denotes (
T15157 7839-7840 NN denotes B
T15158 7840-7841 -RRB- denotes )
T15159 7842-7851 NN denotes Anti-HOIP
T15160 7852-7855 CC denotes and
T15161 7856-7865 JJ denotes anti-FLAG
T15162 7866-7873 JJ denotes Western
T15163 7874-7879 NN denotes blots
T15164 7880-7882 IN denotes of
T15165 7883-7887 NN denotes cell
T15166 7888-7895 NN denotes lysates
T15167 7896-7900 IN denotes from
T15168 7901-7907 NN denotes A20.2J
T15169 7908-7913 NN denotes cells
T15170 7914-7917 CC denotes and
T15171 7918-7932 JJ denotes HOIP-deficient
T15172 7933-7934 -LRB- denotes (
T15173 7934-7941 JJ denotes HOIP-/-
T15174 7941-7942 -RRB- denotes )
T15175 7943-7949 NN denotes clones
T15176 7951-7952 DT denotes A
T15177 7953-7960 JJ denotes partial
T15178 7961-7969 NN denotes decrease
T15179 7970-7972 IN denotes in
T15180 7973-7977 NN denotes HOIP
T15181 7978-7985 NN denotes protein
T15182 7986-7996 NN denotes expression
T15183 7997-7999 VB denotes is
T15184 8000-8007 JJ denotes evident
T15185 8008-8010 IN denotes in
T15186 8011-8016 NN denotes cells
T15187 8017-8026 VB denotes following
T15188 8027-8037 NN denotes disruption
T15189 8038-8040 IN denotes of
T15190 8041-8044 CD denotes one
T15191 8045-8049 NN denotes copy
T15192 8050-8052 IN denotes of
T15193 8053-8058 NN denotes Rnf31
T15194 8059-8060 -LRB- denotes (
T15195 8060-8067 NN denotes HOIP−/+
T15196 8067-8068 -RRB- denotes )
T15197 8070-8074 NN denotes HOIP
T15198 8075-8085 NN denotes expression
T15199 8086-8088 IN denotes in
T15200 8089-8095 NN denotes clones
T15201 8096-8109 VB denotes reconstituted
T15202 8110-8114 IN denotes with
T15203 8115-8117 DT denotes an
T15204 8118-8123 JJ denotes empty
T15205 8124-8134 JJ denotes retroviral
T15206 8135-8141 NN denotes vector
T15207 8142-8143 -LRB- denotes (
T15208 8143-8147 NN denotes pMIP
T15209 8147-8148 -RRB- denotes )
T15210 8149-8151 CC denotes or
T15211 8152-8153 DT denotes a
T15212 8154-8164 JJ denotes retroviral
T15213 8165-8171 NN denotes vector
T15214 8172-8180 VB denotes encoding
T15215 8181-8192 JJ denotes FLAG-tagged
T15216 8193-8197 NN denotes HOIP
T15217 8198-8200 VB denotes is
T15218 8201-8205 RB denotes also
T15219 8206-8211 VB denotes shown
T15220 8213-8224 JJ denotes Approximate
T15221 8225-8234 JJ denotes molecular
T15222 8235-8241 NN denotes weight
T15223 8242-8244 IN denotes of
T15224 8245-8249 NN denotes HOIP
T15225 8250-8252 VB denotes is
T15226 8253-8256 CD denotes 120
T15227 8257-8259 NN denotes kD
T3691 8263-8265 TO denotes To
T3692 8266-8272 VB denotes enable
T3693 8273-8275 PRP denotes us
T3694 8276-8278 TO denotes to
T3695 8279-8286 VB denotes confirm
T3696 8287-8291 IN denotes that
T3697 8292-8295 DT denotes any
T3698 8296-8305 NN denotes signaling
T3699 8306-8308 CC denotes or
T3700 8309-8319 JJ denotes functional
T3701 8320-8327 NN denotes defects
T3702 8328-8336 VB denotes observed
T3703 8337-8339 IN denotes in
T3704 8340-8354 JJ denotes HOIP-deficient
T3705 8355-8360 NN denotes cells
T3706 8361-8365 VB denotes were
T3707 8366-8369 JJ denotes due
T3708 8370-8372 TO denotes to
T3709 8373-8383 NN denotes disruption
T3710 8384-8386 IN denotes of
T3711 8387-8390 DT denotes the
T3712 8391-8396 NN denotes Rnf31
T3713 8397-8401 NN denotes gene
T3714 8401-8402 -COMMA- denotes ,
T3715 8403-8407 DT denotes both
T3716 8408-8422 JJ denotes HOIP-deficient
T3717 8423-8427 NN denotes cell
T3718 8428-8433 NN denotes lines
T3719 8434-8438 VB denotes were
T3720 8439-8449 VB denotes transduced
T3721 8450-8454 IN denotes with
T3722 8455-8456 DT denotes a
T3723 8457-8467 NN denotes retrovirus
T3724 8468-8476 VB denotes encoding
T3725 8477-8488 JJ denotes FLAG-tagged
T3726 8489-8498 JJ denotes wild-type
T3727 8499-8503 NN denotes HOIP
T3728 8503-8504 -COMMA- denotes ,
T3729 8505-8509 RB denotes thus
T3730 8510-8519 VB denotes restoring
T3731 8520-8524 NN denotes HOIP
T3732 8525-8532 NN denotes protein
T3733 8533-8543 NN denotes expression
T3734 8544-8545 -LRB- denotes (
T3735 8545-8549 NN denotes Fig.
T3736 8550-8552 NN denotes 1B
T3737 8552-8553 -RRB- denotes )
T3738 8555-8560 DT denotes These
T3739 8561-8566 NN denotes cells
T3740 8567-8570 VB denotes are
T3741 8571-8579 VB denotes referred
T3742 8580-8582 TO denotes to
T3743 8583-8585 IN denotes as
T3744 8586-8604 JJ denotes HOIP-reconstituted
T3745 8605-8610 NN denotes cells
T3746 8612-8614 IN denotes In
T3747 8615-8618 DT denotes the
T3748 8619-8630 NN denotes experiments
T3749 8631-8635 WDT denotes that
T3750 8636-8642 VB denotes follow
T3751 8642-8643 -COMMA- denotes ,
T3752 8644-8653 NN denotes responses
T3753 8654-8656 IN denotes by
T3754 8657-8662 DT denotes these
T3755 8663-8668 NN denotes cells
T3756 8669-8673 VB denotes were
T3757 8674-8682 VB denotes compared
T3758 8683-8685 TO denotes to
T3759 8686-8691 DT denotes those
T3760 8692-8694 IN denotes of
T3761 8695-8701 NN denotes A20.2J
T3762 8702-8707 NN denotes cells
T3763 8708-8711 CC denotes and
T3764 8712-8726 JJ denotes HOIP-deficient
T3765 8727-8732 NN denotes cells
T3766 8733-8743 VB denotes transduced
T3767 8744-8748 IN denotes with
T3768 8749-8750 DT denotes a
T3769 8751-8761 JJ denotes retroviral
T3770 8762-8768 NN denotes vector
T3771 8769-8770 -LRB- denotes (
T3772 8770-8774 NN denotes pMIP
T3773 8774-8775 -RRB- denotes )
T3774 8776-8783 VB denotes lacking
T3775 8784-8785 DT denotes a
T3776 8786-8790 NN denotes cDNA
T3777 8791-8797 NN denotes insert
T3778 8798-8799 -LRB- denotes (
T3779 8799-8804 JJ denotes empty
T3780 8805-8811 NN denotes vector
T3781 8811-8812 -RRB- denotes )
T4639 8815-8819 NN denotes HOIP
T4640 8820-8822 VB denotes is
T4641 8823-8831 VB denotes required
T4642 8832-8835 IN denotes for
T4643 8836-8849 JJ denotes CD40-mediated
T4644 8850-8854 NN denotes CD80
T4645 8855-8867 NN denotes upregulation
T4646 8868-8871 CC denotes and
T4647 8872-8882 NN denotes activation
T4648 8883-8885 IN denotes of
T4649 8886-8894 NN denotes germline
T4650 8895-8902 NN denotes epsilon
T4651 8903-8916 NN denotes transcription
T4652 8917-8927 NN denotes Engagement
T4653 8928-8930 IN denotes of
T4654 8931-8935 NN denotes CD40
T4655 8936-8938 IN denotes on
T4656 8939-8940 NN denotes B
T4657 8941-8946 NN denotes cells
T4658 8947-8958 VB denotes upregulates
T4659 8959-8969 NN denotes expression
T4660 8970-8972 IN denotes of
T4661 8973-8977 NN denotes CD80
T4662 8978-8979 -LRB- denotes [
T4663 8979-8981 CD denotes 10
T4664 8981-8982 -RRB- denotes ]
T4665 8982-8983 -COMMA- denotes ,
T4666 8984-8985 DT denotes a
T4667 8986-8990 NN denotes cell
T4668 8991-8998 NN denotes surface
T4669 8999-9006 NN denotes protein
T4670 9007-9011 WDT denotes that
T4671 9012-9020 VB denotes promotes
T4672 9021-9031 NN denotes activation
T4673 9032-9034 IN denotes of
T4674 9035-9036 NN denotes T
T4675 9037-9042 NN denotes cells
T4676 9043-9054 VB denotes interacting
T4677 9055-9059 IN denotes with
T4678 9060-9061 NN denotes B
T4679 9062-9067 NN denotes cells
T4680 9068-9071 CC denotes and
T4681 9072-9077 JJ denotes other
T4682 9078-9081 NN denotes APC
T4683 9083-9085 TO denotes To
T4684 9086-9091 VB denotes begin
T4685 9092-9102 VB denotes evaluating
T4686 9103-9112 JJ denotes potential
T4687 9113-9126 NN denotes contributions
T4688 9127-9129 IN denotes of
T4689 9130-9134 NN denotes HOIP
T4690 9135-9137 TO denotes to
T4691 9138-9142 NN denotes CD40
T4692 9143-9152 NN denotes signaling
T4693 9152-9153 -COMMA- denotes ,
T4694 9154-9156 PRP denotes we
T4695 9157-9164 VB denotes assayed
T4696 9165-9168 DT denotes the
T4697 9169-9181 NN denotes upregulation
T4698 9182-9184 IN denotes of
T4699 9185-9189 NN denotes CD80
T4700 9190-9197 VB denotes induced
T4701 9198-9200 IN denotes by
T4702 9201-9211 NN denotes engagement
T4703 9212-9214 IN denotes of
T4704 9215-9219 NN denotes CD40
T4705 9220-9222 IN denotes on
T4706 9223-9231 JJ denotes parental
T4707 9232-9238 NN denotes A20.2J
T4708 9238-9239 -COMMA- denotes ,
T4709 9240-9254 JJ denotes HOIP-deficient
T4710 9254-9255 -COMMA- denotes ,
T4711 9256-9259 CC denotes and
T4712 9260-9278 JJ denotes HOIP-reconstituted
T4713 9279-9284 NN denotes cells
T4714 9285-9286 -LRB- denotes (
T4715 9286-9290 NN denotes Fig.
T4716 9291-9292 CD denotes 2
T4717 9292-9293 -RRB- denotes )
T4718 9295-9303 VB denotes Compared
T4719 9304-9306 TO denotes to
T4720 9307-9310 DT denotes the
T4721 9311-9319 JJ denotes parental
T4722 9320-9324 NN denotes cell
T4723 9325-9329 NN denotes line
T4724 9329-9330 -COMMA- denotes ,
T4725 9331-9334 DT denotes the
T4726 9335-9350 JJ denotes CD40-stimulated
T4727 9351-9363 NN denotes upregulation
T4728 9364-9366 IN denotes of
T4729 9367-9371 NN denotes CD80
T4730 9372-9375 VB denotes was
T4731 9376-9388 RB denotes dramatically
T4732 9389-9396 VB denotes reduced
T4733 9397-9399 IN denotes in
T4734 9400-9403 DT denotes all
T4735 9404-9418 JJ denotes HOIP-deficient
T4736 9419-9424 NN denotes cells
T4737 9425-9431 VB denotes tested
T4738 9433-9435 IN denotes In
T4739 9436-9444 NN denotes contrast
T4740 9444-9445 -COMMA- denotes ,
T4741 9446-9458 NN denotes upregulation
T4742 9459-9461 IN denotes of
T4743 9462-9466 NN denotes CD80
T4744 9467-9470 VB denotes was
T4745 9471-9479 VB denotes restored
T4746 9480-9482 IN denotes in
T4747 9483-9501 JJ denotes HOIP-reconstituted
T4748 9502-9507 NN denotes cells
T4749 9507-9508 -COMMA- denotes ,
T4750 9509-9519 VB denotes confirming
T4751 9520-9524 IN denotes that
T4752 9525-9528 DT denotes the
T4753 9529-9535 NN denotes defect
T4754 9536-9538 IN denotes in
T4755 9539-9543 NN denotes CD80
T4756 9544-9556 NN denotes upregulation
T4757 9557-9559 IN denotes in
T4758 9560-9563 DT denotes the
T4759 9564-9578 JJ denotes HOIP-deficient
T4760 9579-9584 NN denotes cells
T4761 9585-9588 VB denotes was
T4762 9589-9592 JJ denotes due
T4763 9593-9595 TO denotes to
T4764 9596-9599 DT denotes the
T4765 9600-9610 NN denotes disruption
T4766 9611-9613 IN denotes of
T4767 9614-9619 NN denotes Rnf31
T15490 9665-9678 JJ denotes CD40-mediated
T15491 9679-9683 NN denotes CD80
T15492 9684-9696 NN denotes upregulation
T15493 9697-9699 VB denotes is
T15494 9700-9709 JJ denotes defective
T15495 9710-9712 IN denotes in
T15496 9713-9727 JJ denotes HOIP-deficient
T15497 9728-9733 NN denotes cells
T15498 9735-9740 NN denotes Cells
T15499 9741-9745 VB denotes were
T15500 9746-9755 VB denotes incubated
T15501 9756-9759 IN denotes for
T15502 9760-9762 CD denotes 72
T15503 9763-9766 NN denotes hrs
T15504 9767-9771 IN denotes with
T15505 9772-9774 DT denotes an
T15506 9775-9782 NN denotes isotype
T15507 9783-9790 NN denotes control
T15508 9791-9799 NN denotes antibody
T15509 9800-9801 -LRB- denotes (
T15510 9801-9805 JJ denotes left
T15511 9805-9806 -RRB- denotes )
T15512 9807-9809 CC denotes or
T15513 9810-9819 JJ denotes anti-CD40
T15514 9820-9828 NN denotes antibody
T15515 9829-9830 -LRB- denotes (
T15516 9830-9835 NN denotes right
T15517 9835-9836 -RRB- denotes )
T15518 9837-9840 CC denotes and
T15519 9841-9845 RB denotes then
T15520 9846-9853 VB denotes stained
T15521 9854-9857 IN denotes for
T15522 9858-9868 NN denotes expression
T15523 9869-9871 IN denotes of
T15524 9872-9876 NN denotes CD80
T15525 9877-9878 -LRB- denotes (
T15526 9878-9884 VB denotes filled
T15527 9885-9893 NN denotes profiles
T15528 9894-9902 VB denotes indicate
T15529 9903-9911 NN denotes staining
T15530 9912-9916 IN denotes with
T15531 9917-9926 JJ denotes anti-CD80
T15532 9927-9935 NN denotes antibody
T15533 9935-9936 -COLON- denotes ;
T15534 9937-9941 JJ denotes open
T15535 9942-9950 NN denotes profiles
T15536 9951-9954 VB denotes are
T15537 9955-9963 VB denotes staining
T15538 9964-9968 IN denotes with
T15539 9969-9971 DT denotes an
T15540 9972-9979 NN denotes isotype
T15541 9980-9987 NN denotes control
T15542 9988-9996 NN denotes antibody
T15543 9996-9997 -RRB- denotes )
T15544 9999-10011 NN denotes Transduction
T15545 10012-10014 IN denotes of
T15546 10015-10019 CC denotes both
T15547 10020-10034 JJ denotes HOIP-deficient
T15548 10035-10036 -LRB- denotes (
T15549 10036-10043 JJ denotes HOIP-/-
T15550 10043-10044 -RRB- denotes )
T15551 10045-10051 NN denotes clones
T15552 10052-10056 IN denotes with
T15553 10057-10058 DT denotes a
T15554 10059-10069 NN denotes retrovirus
T15555 10070-10078 VB denotes encoding
T15556 10079-10088 NN denotes FLAG-HOIP
T15557 10089-10097 VB denotes reversed
T15558 10098-10107 JJ denotes defective
T15559 10108-10112 NN denotes CD80
T15560 10113-10125 NN denotes upregulation
T15561 10125-10126 -COMMA- denotes ,
T15562 10127-10132 IN denotes while
T15563 10133-10145 NN denotes transduction
T15564 10146-10150 IN denotes with
T15565 10151-10153 DT denotes an
T15566 10154-10159 JJ denotes empty
T15567 10160-10166 NN denotes vector
T15568 10167-10168 -LRB- denotes (
T15569 10168-10172 NN denotes pMIP
T15570 10172-10173 -RRB- denotes )
T15571 10174-10177 VB denotes did
T15572 10178-10181 RB denotes not
T15573 10183-10190 JJ denotes Similar
T15574 10191-10198 NN denotes results
T15575 10199-10203 VB denotes were
T15576 10204-10212 VB denotes obtained
T15577 10213-10215 IN denotes in
T15578 10216-10217 DT denotes a
T15579 10218-10224 JJ denotes second
T15580 10225-10235 NN denotes experiment
T4768 10239-10243 NN denotes CD40
T4769 10244-10251 NN denotes signals
T4770 10252-10254 IN denotes in
T4771 10255-10256 NN denotes B
T4772 10257-10262 NN denotes cells
T4773 10263-10266 MD denotes can
T4774 10267-10271 RB denotes also
T4775 10272-10282 VB denotes contribute
T4776 10283-10285 TO denotes to
T4777 10286-10289 DT denotes the
T4778 10290-10300 NN denotes activation
T4779 10301-10303 IN denotes of
T4780 10304-10307 NN denotes DNA
T4781 10308-10321 NN denotes recombination
T4782 10322-10324 IN denotes in
T4783 10325-10328 DT denotes the
T4784 10329-10343 NN denotes immunoglobulin
T4785 10344-10349 JJ denotes heavy
T4786 10350-10355 NN denotes chain
T4787 10356-10361 NN denotes locus
T4788 10362-10363 -LRB- denotes [
T4789 10363-10365 CD denotes 11
T4790 10365-10366 -RRB- denotes ]
T4791 10366-10367 -COMMA- denotes ,
T4792 10368-10369 -LRB- denotes [
T4793 10369-10371 CD denotes 12
T4794 10371-10372 -RRB- denotes ]
T4795 10374-10378 DT denotes This
T4796 10379-10386 NN denotes process
T4797 10386-10387 -COMMA- denotes ,
T4798 10388-10393 VB denotes known
T4799 10394-10396 IN denotes as
T4800 10397-10411 NN denotes immunoglobulin
T4801 10412-10417 NN denotes class
T4802 10418-10427 NN denotes switching
T4803 10428-10429 -LRB- denotes (
T4804 10429-10431 CC denotes or
T4805 10432-10439 NN denotes isotype
T4806 10440-10449 NN denotes switching
T4807 10449-10450 -RRB- denotes )
T4808 10450-10451 -COMMA- denotes ,
T4809 10452-10458 VB denotes allows
T4810 10459-10460 NN denotes B
T4811 10461-10466 NN denotes cells
T4812 10466-10467 -COMMA- denotes ,
T4813 10468-10477 RB denotes initially
T4814 10478-10488 VB denotes expressing
T4815 10489-10492 NN denotes IgM
T4816 10492-10493 -COMMA- denotes ,
T4817 10494-10496 TO denotes to
T4818 10497-10503 VB denotes switch
T4819 10504-10506 TO denotes to
T4820 10507-10510 NN denotes IgA
T4821 10510-10511 -COMMA- denotes ,
T4822 10512-10515 NN denotes IgG
T4823 10515-10516 -COMMA- denotes ,
T4824 10517-10519 CC denotes or
T4825 10520-10523 NN denotes IgE
T4826 10524-10533 VB denotes depending
T4827 10534-10536 IN denotes on
T4828 10537-10540 DT denotes the
T4829 10541-10546 NN denotes class
T4830 10547-10549 IN denotes of
T4831 10550-10558 NN denotes antibody
T4832 10559-10563 RB denotes most
T4833 10564-10575 JJ denotes appropriate
T4834 10576-10579 IN denotes for
T4835 10580-10581 DT denotes a
T4836 10582-10592 JJ denotes particular
T4837 10593-10599 JJ denotes immune
T4838 10600-10608 NN denotes response
T4839 10610-10619 VB denotes Switching
T4840 10620-10622 TO denotes to
T4841 10623-10626 NN denotes IgE
T4842 10627-10630 MD denotes can
T4843 10631-10633 VB denotes be
T4844 10634-10641 VB denotes induced
T4845 10642-10644 IN denotes by
T4846 10645-10648 DT denotes the
T4847 10649-10660 NN denotes combination
T4848 10661-10663 IN denotes of
T4849 10664-10668 NN denotes IL-4
T4850 10669-10672 CC denotes and
T4851 10673-10677 NN denotes CD40
T4852 10678-10685 NN denotes signals
T4853 10687-10690 DT denotes The
T4854 10691-10695 NN denotes gene
T4855 10696-10709 NN denotes rearrangement
T4856 10710-10719 JJ denotes necessary
T4857 10720-10723 IN denotes for
T4858 10724-10727 NN denotes IgE
T4859 10728-10738 NN denotes production
T4860 10739-10741 VB denotes is
T4861 10742-10750 VB denotes preceded
T4862 10751-10753 IN denotes by
T4863 10754-10757 DT denotes the
T4864 10758-10768 NN denotes production
T4865 10769-10771 IN denotes of
T4866 10772-10782 JJ denotes non-coding
T4867 10783-10786 NN denotes RNA
T4868 10787-10798 NN denotes transcripts
T4869 10799-10803 IN denotes from
T4870 10804-10812 NN denotes germline
T4871 10813-10822 NN denotes sequences
T4872 10823-10825 IN denotes in
T4873 10826-10829 DT denotes the
T4874 10830-10833 NN denotes IgE
T4875 10834-10839 JJ denotes heavy
T4876 10840-10845 NN denotes chain
T4877 10846-10851 NN denotes locus
T4878 10852-10853 -LRB- denotes [
T4879 10853-10855 CD denotes 13
T4880 10855-10856 -RRB- denotes ]
T4881 10858-10860 TO denotes To
T4882 10861-10870 VB denotes determine
T4883 10871-10878 IN denotes whether
T4884 10879-10883 NN denotes HOIP
T4885 10884-10886 VB denotes is
T4886 10887-10895 VB denotes required
T4887 10896-10898 IN denotes in
T4888 10899-10902 DT denotes the
T4889 10903-10913 NN denotes initiation
T4890 10914-10916 IN denotes of
T4891 10917-10920 DT denotes the
T4892 10921-10926 NN denotes class
T4893 10927-10936 NN denotes switching
T4894 10937-10944 NN denotes process
T4895 10944-10945 -COMMA- denotes ,
T4896 10946-10949 DT denotes the
T4897 10950-10958 JJ denotes relevant
T4898 10959-10960 NN denotes B
T4899 10961-10965 NN denotes cell
T4900 10966-10971 NN denotes lines
T4901 10972-10976 VB denotes were
T4902 10977-10987 VB denotes stimulated
T4903 10988-10995 IN denotes through
T4904 10996-11000 NN denotes CD40
T4905 11001-11003 IN denotes in
T4906 11004-11007 DT denotes the
T4907 11008-11016 NN denotes presence
T4908 11017-11019 IN denotes of
T4909 11020-11024 NN denotes IL-4
T4910 11025-11028 CC denotes and
T4911 11029-11035 VB denotes tested
T4912 11036-11039 IN denotes for
T4913 11040-11050 NN denotes production
T4914 11051-11053 IN denotes of
T4915 11054-11062 NN denotes germline
T4916 11063-11070 NN denotes epsilon
T4917 11071-11072 -LRB- denotes (
T4918 11072-11075 NN denotes GLε
T4919 11075-11076 -RRB- denotes )
T4920 11077-11088 NN denotes transcripts
T4921 11089-11090 -LRB- denotes (
T4922 11090-11094 NN denotes Fig.
T4923 11095-11096 CD denotes 3
T4924 11096-11097 -RRB- denotes )
T4925 11099-11108 JJ denotes Anti-CD40
T4926 11109-11117 NN denotes antibody
T4927 11118-11129 NN denotes stimulation
T4928 11130-11135 RB denotes alone
T4929 11136-11138 CC denotes or
T4930 11139-11147 RB denotes together
T4931 11148-11152 IN denotes with
T4932 11153-11157 NN denotes IL-4
T4933 11158-11165 VB denotes induced
T4934 11166-11169 NN denotes GLε
T4935 11170-11183 NN denotes transcription
T4936 11184-11186 IN denotes in
T4937 11187-11195 JJ denotes parental
T4938 11196-11202 NN denotes A20.2J
T4939 11203-11208 NN denotes cells
T4940 11209-11211 IN denotes as
T4941 11212-11220 VB denotes expected
T4942 11222-11224 IN denotes In
T4943 11225-11233 NN denotes contrast
T4944 11233-11234 -COMMA- denotes ,
T4945 11235-11239 RB denotes only
T4946 11240-11243 JJ denotes low
T4947 11244-11250 NN denotes levels
T4948 11251-11253 IN denotes of
T4949 11254-11257 NN denotes GLε
T4950 11258-11268 NN denotes expression
T4951 11269-11273 VB denotes were
T4952 11274-11282 VB denotes detected
T4953 11283-11285 IN denotes in
T4954 11286-11300 JJ denotes HOIP-deficient
T4955 11301-11306 NN denotes cells
T4956 11307-11317 VB denotes stimulated
T4957 11318-11322 IN denotes with
T4958 11323-11329 CC denotes either
T4959 11330-11339 JJ denotes anti-CD40
T4960 11340-11348 NN denotes antibody
T4961 11349-11354 RB denotes alone
T4962 11355-11357 CC denotes or
T4963 11358-11367 NN denotes anti-CD40
T4964 11368-11371 CC denotes and
T4965 11372-11376 NN denotes IL-4
T4966 11378-11384 JJ denotes Normal
T4967 11385-11391 NN denotes levels
T4968 11392-11394 IN denotes of
T4969 11395-11398 NN denotes GLε
T4970 11399-11409 NN denotes expression
T4971 11410-11414 VB denotes were
T4972 11415-11422 VB denotes induced
T4973 11423-11425 IN denotes in
T4974 11426-11444 JJ denotes HOIP-reconstituted
T4975 11445-11450 NN denotes cells
T4976 11451-11461 VB denotes stimulated
T4977 11462-11466 IN denotes with
T4978 11467-11476 JJ denotes anti-CD40
T4979 11477-11485 NN denotes antibody
T4980 11486-11491 RB denotes alone
T4981 11492-11494 CC denotes or
T4982 11495-11503 RB denotes together
T4983 11504-11508 IN denotes with
T4984 11509-11513 NN denotes IL-4
T4985 11515-11527 RB denotes Collectively
T4986 11527-11528 -COMMA- denotes ,
T4987 11529-11534 DT denotes these
T4988 11535-11539 NN denotes data
T4989 11540-11551 VB denotes demonstrate
T4990 11552-11556 IN denotes that
T4991 11557-11561 NN denotes HOIP
T4992 11562-11565 VB denotes has
T4993 11566-11567 DT denotes a
T4994 11568-11576 JJ denotes critical
T4995 11577-11581 NN denotes role
T4996 11582-11584 IN denotes in
T4997 11585-11593 JJ denotes cellular
T4998 11594-11603 NN denotes responses
T4999 11604-11606 TO denotes to
T5000 11607-11611 NN denotes CD40
T5001 11612-11621 NN denotes signaling
T15889 11667-11670 NN denotes GLε
T15890 11671-11684 NN denotes transcription
T15891 11685-11687 VB denotes is
T15892 11688-11697 JJ denotes defective
T15893 11698-11700 IN denotes in
T15894 11701-11716 JJ denotes CD40-stimulated
T15895 11717-11731 JJ denotes HOIP-deficient
T15896 11732-11737 NN denotes cells
T15897 11739-11745 NN denotes A20.2J
T15898 11746-11749 CC denotes and
T15899 11750-11764 JJ denotes HOIP-deficient
T15900 11765-11770 NN denotes cells
T15901 11771-11781 VB denotes transduced
T15902 11782-11786 IN denotes with
T15903 11787-11789 DT denotes an
T15904 11790-11795 JJ denotes empty
T15905 11796-11806 JJ denotes retroviral
T15906 11807-11813 NN denotes vector
T15907 11814-11815 -LRB- denotes (
T15908 11815-11821 NN denotes A20.2J
T15909 11822-11823 SYM denotes +
T15910 11824-11828 NN denotes pMIP
T15911 11829-11832 CC denotes and
T15912 11833-11840 JJ denotes HOIP-/-
T15913 11841-11842 SYM denotes +
T15914 11843-11847 NN denotes pMIP
T15915 11847-11848 -COMMA- denotes ,
T15916 11849-11861 RB denotes respectively
T15917 11861-11862 -RRB- denotes )
T15918 11862-11863 -COMMA- denotes ,
T15919 11864-11866 CC denotes or
T15920 11867-11881 JJ denotes HOIP-deficient
T15921 11882-11887 NN denotes cells
T15922 11888-11898 VB denotes transduced
T15923 11899-11903 IN denotes with
T15924 11904-11905 DT denotes a
T15925 11906-11919 JJ denotes HOIP-encoding
T15926 11920-11930 NN denotes retrovirus
T15927 11931-11932 -LRB- denotes (
T15928 11932-11939 JJ denotes HOIP-/-
T15929 11940-11941 SYM denotes +
T15930 11942-11946 NN denotes HOIP
T15931 11946-11947 -RRB- denotes )
T15932 11948-11952 VB denotes were
T15933 11953-11961 VB denotes cultured
T15934 11962-11971 RB denotes overnight
T15935 11972-11976 IN denotes with
T15936 11977-11986 JJ denotes agonistic
T15937 11987-11996 NN denotes anti-CD40
T15938 11997-11999 CC denotes or
T15939 12000-12002 DT denotes an
T15940 12003-12010 NN denotes isotype
T15941 12011-12018 NN denotes control
T15942 12019-12027 NN denotes antibody
T15943 12028-12029 -LRB- denotes (
T15944 12029-12032 NN denotes iso
T15945 12032-12033 -RRB- denotes )
T15946 12033-12034 -COMMA- denotes ,
T15947 12035-12039 IN denotes with
T15948 12040-12042 CC denotes or
T15949 12043-12050 IN denotes without
T15950 12051-12055 NN denotes IL-4
T15951 12057-12060 NN denotes RNA
T15952 12061-12064 VB denotes was
T15953 12065-12073 VB denotes isolated
T15954 12074-12078 IN denotes from
T15955 12079-12082 DT denotes the
T15956 12083-12088 NN denotes cells
T15957 12088-12089 -COMMA- denotes ,
T15958 12090-12109 JJ denotes reverse-transcribed
T15959 12109-12110 -COMMA- denotes ,
T15960 12111-12114 CC denotes and
T15961 12115-12119 RB denotes then
T15962 12120-12129 VB denotes subjected
T15963 12130-12132 TO denotes to
T15964 12133-12145 JJ denotes quantitative
T15965 12146-12149 NN denotes PCR
T15966 12150-12152 TO denotes to
T15967 12153-12162 VB denotes determine
T15968 12163-12169 NN denotes levels
T15969 12170-12172 IN denotes of
T15970 12173-12176 NN denotes GLε
T15971 12177-12188 NN denotes transcripts
T15972 12190-12197 NN denotes Results
T15973 12198-12202 VB denotes were
T15974 12203-12213 VB denotes normalized
T15975 12214-12216 TO denotes to
T15976 12217-12220 DT denotes the
T15977 12221-12227 NN denotes levels
T15978 12228-12230 IN denotes of
T15979 12231-12236 NN denotes Hprt1
T15980 12237-12248 NN denotes transcripts
T15981 12249-12251 IN denotes in
T15982 12252-12256 DT denotes each
T15983 12257-12263 NN denotes sample
T15984 12265-12272 NN denotes Symbols
T15985 12273-12281 VB denotes indicate
T15986 12282-12285 DT denotes the
T15987 12286-12292 NN denotes values
T15988 12293-12297 IN denotes from
T15989 12298-12307 NN denotes duplicate
T15990 12308-12316 NN denotes cultures
T15991 12317-12318 -LRB- denotes (
T15992 12318-12319 DT denotes a
T15993 12320-12324 NN denotes line
T15994 12325-12334 VB denotes indicates
T15995 12335-12338 DT denotes the
T15996 12339-12343 NN denotes mean
T15997 12344-12346 IN denotes of
T15998 12347-12350 DT denotes the
T15999 12351-12354 CD denotes two
T16000 12355-12361 NN denotes values
T16001 12361-12362 -RRB- denotes )
T16002 12364-12371 JJ denotes Similar
T16003 12372-12379 NN denotes results
T16004 12380-12384 VB denotes were
T16005 12385-12393 VB denotes obtained
T16006 12394-12396 IN denotes in
T16007 12397-12398 DT denotes a
T16008 12399-12405 JJ denotes second
T16009 12406-12416 NN denotes experiment
T16010 12417-12420 CC denotes and
T16011 12421-12423 IN denotes in
T16012 12424-12426 DT denotes an
T16013 12427-12437 JJ denotes additional
T16014 12438-12448 NN denotes experiment
T16015 12449-12453 IN denotes with
T16016 12454-12455 DT denotes a
T16017 12456-12462 JJ denotes second
T16018 12463-12477 JJ denotes HOIP-deficient
T16019 12478-12483 NN denotes clone
T5789 12486-12490 NN denotes HOIP
T5790 12491-12499 VB denotes mediates
T5791 12500-12515 JJ denotes CD40-stimulated
T5792 12516-12521 NN denotes NF-κB
T5793 12522-12525 CC denotes and
T5794 12526-12529 NN denotes JNK
T5795 12530-12540 NN denotes activation
T5796 12541-12544 DT denotes The
T5797 12545-12552 NN denotes results
T5798 12553-12562 VB denotes described
T5799 12563-12568 IN denotes above
T5800 12569-12573 VB denotes show
T5801 12574-12578 IN denotes that
T5802 12579-12583 NN denotes HOIP
T5803 12584-12589 VB denotes plays
T5804 12590-12592 DT denotes an
T5805 12593-12602 JJ denotes important
T5806 12603-12607 NN denotes role
T5807 12608-12610 IN denotes in
T5808 12611-12624 JJ denotes CD40-mediated
T5809 12625-12633 NN denotes effector
T5810 12634-12643 NN denotes functions
T5811 12644-12646 IN denotes of
T5812 12647-12648 NN denotes B
T5813 12649-12654 NN denotes cells
T5814 12656-12658 PRP denotes It
T5815 12659-12666 VB denotes follows
T5816 12667-12671 IN denotes that
T5817 12672-12676 NN denotes HOIP
T5818 12677-12679 VB denotes is
T5819 12680-12686 RB denotes likely
T5820 12687-12688 DT denotes a
T5821 12689-12692 JJ denotes key
T5822 12693-12701 NN denotes mediator
T5823 12702-12704 IN denotes of
T5824 12705-12709 NN denotes CD40
T5825 12710-12719 NN denotes signaling
T5826 12721-12723 TO denotes To
T5827 12724-12728 VB denotes test
T5828 12729-12733 DT denotes this
T5829 12734-12744 NN denotes hypothesis
T5830 12744-12745 -COMMA- denotes ,
T5831 12746-12748 PRP denotes we
T5832 12749-12759 VB denotes stimulated
T5833 12760-12766 NN denotes A20.2J
T5834 12767-12770 CC denotes and
T5835 12771-12785 JJ denotes HOIP-deficient
T5836 12786-12791 NN denotes cells
T5837 12792-12796 IN denotes with
T5838 12797-12802 NN denotes CD154
T5839 12803-12804 -LRB- denotes (
T5840 12804-12808 NN denotes CD40
T5841 12809-12815 NN denotes ligand
T5842 12815-12816 -RRB- denotes )
T5843 12817-12826 VB denotes expressed
T5844 12827-12829 IN denotes by
T5845 12830-12833 NN denotes HI5
T5846 12834-12840 NN denotes insect
T5847 12841-12846 NN denotes cells
T5848 12847-12848 -LRB- denotes [
T5849 12848-12849 CD denotes 7
T5850 12849-12850 -RRB- denotes ]
T5851 12850-12851 -COMMA- denotes ,
T5852 12852-12853 -LRB- denotes [
T5853 12853-12854 CD denotes 8
T5854 12854-12855 -RRB- denotes ]
T5855 12856-12859 CC denotes and
T5856 12860-12868 VB denotes measured
T5857 12869-12879 NN denotes activation
T5858 12880-12882 IN denotes of
T5859 12883-12886 DT denotes the
T5860 12887-12892 NN denotes NF-κB
T5861 12893-12896 CC denotes and
T5862 12897-12900 NN denotes JNK
T5863 12901-12909 NN denotes pathways
T5864 12909-12910 -COMMA- denotes ,
T5865 12911-12914 CD denotes two
T5866 12915-12917 IN denotes of
T5867 12918-12921 DT denotes the
T5868 12922-12927 JJ denotes major
T5869 12928-12943 JJ denotes transcriptional
T5870 12944-12954 NN denotes regulators
T5871 12955-12964 VB denotes activated
T5872 12965-12967 IN denotes by
T5873 12968-12972 NN denotes CD40
T5874 12973-12974 -LRB- denotes [
T5875 12974-12975 CD denotes 4
T5876 12975-12976 -RRB- denotes ]
T5877 12978-12993 JJ denotes Cell-associated
T5878 12994-12999 NN denotes CD154
T5879 13000-13003 VB denotes was
T5880 13004-13008 VB denotes used
T5881 13009-13011 IN denotes as
T5882 13012-13015 DT denotes the
T5883 13016-13024 NN denotes stimulus
T5884 13025-13027 IN denotes in
T5885 13028-13033 DT denotes these
T5886 13034-13045 NN denotes experiments
T5887 13046-13048 IN denotes as
T5888 13049-13051 PRP denotes it
T5889 13052-13061 RB denotes typically
T5890 13062-13070 VB denotes provides
T5891 13071-13075 RB denotes more
T5892 13076-13082 JJ denotes robust
T5893 13082-13083 -COMMA- denotes ,
T5894 13084-13087 CC denotes and
T5895 13088-13097 RB denotes therefore
T5896 13098-13102 RB denotes more
T5897 13103-13110 RB denotes readily
T5898 13111-13119 VB denotes detected
T5899 13119-13120 -COMMA- denotes ,
T5900 13121-13131 NN denotes activation
T5901 13132-13139 NN denotes signals
T5902 13140-13144 IN denotes than
T5903 13145-13149 VB denotes does
T5904 13150-13159 JJ denotes anti-CD40
T5905 13160-13168 NN denotes antibody
T5906 13170-13180 NN denotes Activation
T5907 13181-13183 IN denotes of
T5908 13184-13187 DT denotes the
T5909 13188-13197 JJ denotes canonical
T5910 13198-13203 NN denotes NF-κB
T5911 13204-13211 NN denotes pathway
T5912 13212-13214 VB denotes is
T5913 13215-13224 VB denotes initiated
T5914 13225-13229 IN denotes with
T5915 13230-13233 DT denotes the
T5916 13234-13249 NN denotes phosphorylation
T5917 13250-13252 IN denotes of
T5918 13253-13256 NN denotes IκB
T5919 13257-13265 NN denotes proteins
T5920 13266-13268 IN denotes by
T5921 13269-13272 DT denotes the
T5922 13273-13276 NN denotes IκB
T5923 13277-13283 NN denotes kinase
T5924 13284-13291 NN denotes complex
T5925 13292-13293 -LRB- denotes (
T5926 13293-13296 NN denotes IKK
T5927 13296-13297 -RRB- denotes )
T5928 13299-13301 IN denotes In
T5929 13302-13309 VB denotes resting
T5930 13310-13315 NN denotes cells
T5931 13315-13316 -COMMA- denotes ,
T5932 13317-13320 NN denotes IκB
T5933 13321-13329 NN denotes proteins
T5934 13330-13333 VB denotes are
T5935 13334-13345 JJ denotes responsible
T5936 13346-13349 IN denotes for
T5937 13350-13362 VB denotes sequestering
T5938 13363-13368 NN denotes NF-κB
T5939 13369-13377 NN denotes subunits
T5940 13378-13380 IN denotes in
T5941 13381-13384 DT denotes the
T5942 13385-13394 NN denotes cytoplasm
T5943 13396-13411 NN denotes Phosphorylation
T5944 13412-13414 IN denotes by
T5945 13415-13418 DT denotes the
T5946 13419-13422 NN denotes IKK
T5947 13423-13430 NN denotes complex
T7483 13423-13430 NN denotes complex
T5948 13431-13438 VB denotes targets
T5949 13439-13442 NN denotes IκB
T5950 13443-13451 NN denotes proteins
T5951 13452-13455 IN denotes for
T5952 13456-13470 NN denotes ubiquitination
T5953 13471-13474 CC denotes and
T5954 13475-13486 NN denotes degradation
T5955 13486-13487 -COMMA- denotes ,
T5956 13488-13496 VB denotes allowing
T5957 13497-13502 NN denotes NF-κB
T5958 13503-13505 TO denotes to
T5959 13506-13511 VB denotes enter
T5960 13512-13515 DT denotes the
T5961 13516-13523 NN denotes nucleus
T5962 13524-13527 CC denotes and
T5963 13528-13536 VB denotes activate
T5964 13537-13541 NN denotes gene
T5965 13542-13552 NN denotes expression
T5966 13554-13567 JJ denotes CD40-mediated
T5967 13568-13583 NN denotes phosphorylation
T5968 13584-13587 CC denotes and
T5969 13588-13599 NN denotes degradation
T5970 13600-13602 IN denotes of
T5971 13603-13607 NN denotes IκBα
T5972 13608-13610 IN denotes in
T5973 13611-13625 JJ denotes HOIP-deficient
T5974 13626-13631 NN denotes cells
T5975 13632-13635 VB denotes was
T5976 13636-13648 RB denotes dramatically
T5977 13649-13657 JJ denotes impaired
T5978 13658-13666 JJ denotes relative
T5979 13667-13669 TO denotes to
T5980 13670-13674 DT denotes that
T5981 13675-13683 VB denotes observed
T5982 13684-13686 IN denotes in
T5983 13687-13695 JJ denotes parental
T5984 13696-13702 NN denotes A20.2J
T5985 13703-13708 NN denotes cells
T5986 13709-13710 -LRB- denotes (
T5987 13710-13714 NN denotes Fig.
T5988 13715-13716 CD denotes 4
T5989 13716-13717 -RRB- denotes )
T5990 13719-13721 PRP denotes We
T5991 13722-13726 RB denotes also
T5992 13727-13734 VB denotes assayed
T5993 13735-13745 NN denotes activation
T5994 13746-13748 IN denotes of
T5995 13749-13752 DT denotes the
T5996 13753-13769 JJ denotes stress-activated
T5997 13770-13777 NN denotes protein
T5998 13778-13784 NN denotes kinase
T5999 13785-13788 NN denotes JNK
T6000 13789-13791 IN denotes in
T6001 13792-13800 NN denotes response
T6002 13801-13803 TO denotes to
T6003 13804-13808 NN denotes CD40
T6004 13809-13819 NN denotes engagement
T6005 13820-13821 -LRB- denotes (
T6006 13821-13825 NN denotes Fig.
T6007 13826-13827 CD denotes 4
T6008 13827-13828 -RRB- denotes )
T6009 13830-13843 JJ denotes CD40-mediated
T6010 13844-13847 NN denotes JNK
T6011 13848-13858 NN denotes activation
T6012 13859-13861 IN denotes in
T6013 13862-13876 JJ denotes HOIP-deficient
T6014 13877-13882 NN denotes cells
T6015 13883-13886 VB denotes was
T6016 13887-13895 VB denotes impaired
T6017 13896-13898 IN denotes as
T6018 13899-13907 VB denotes measured
T6019 13908-13910 IN denotes by
T6020 13911-13926 NN denotes phosphorylation
T6021 13927-13929 IN denotes of
T6022 13930-13936 NN denotes Thr183
T6023 13937-13940 CC denotes and
T6024 13941-13947 NN denotes Tyr185
T6025 13948-13950 IN denotes in
T6026 13951-13954 NN denotes JNK
T6027 13956-13968 JJ denotes CD40-induced
T6028 13969-13979 NN denotes activation
T6029 13980-13982 IN denotes of
T6030 13983-13988 NN denotes NF-κB
T6031 13989-13992 CC denotes and
T6032 13993-13996 NN denotes JNK
T6033 13997-13999 IN denotes in
T6034 14000-14018 JJ denotes HOIP-reconstituted
T6035 14019-14024 NN denotes cells
T6036 14025-14028 VB denotes was
T6037 14029-14035 JJ denotes normal
T6038 14035-14036 -COMMA- denotes ,
T6039 14037-14050 VB denotes demonstrating
T6040 14051-14055 IN denotes that
T6041 14056-14059 DT denotes the
T6042 14060-14067 NN denotes defects
T6043 14068-14076 VB denotes observed
T6044 14077-14079 IN denotes in
T6045 14080-14094 JJ denotes gene-deficient
T6046 14095-14100 NN denotes cells
T6047 14101-14105 VB denotes were
T6048 14106-14109 JJ denotes due
T6049 14110-14112 TO denotes to
T6050 14113-14116 DT denotes the
T6051 14117-14124 NN denotes absence
T6052 14125-14127 IN denotes of
T6053 14128-14132 NN denotes HOIP
T6054 14133-14143 NN denotes expression
T16374 14189-14201 JJ denotes CD40-induced
T16375 14202-14212 NN denotes activation
T16376 14213-14215 IN denotes of
T16377 14216-14221 NN denotes NF-κB
T16378 14222-14225 CC denotes and
T16379 14226-14229 NN denotes JNK
T16380 14230-14232 VB denotes is
T16381 14233-14242 JJ denotes defective
T16382 14243-14245 IN denotes in
T16383 14246-14260 JJ denotes HOIP-deficient
T16384 14261-14266 NN denotes cells
T16385 14268-14281 JJ denotes CD40-mediated
T16386 14282-14291 NN denotes signaling
T16387 14292-14295 VB denotes was
T16388 14296-14305 JJ denotes defective
T16389 14306-14308 IN denotes in
T16390 14309-14323 JJ denotes HOIP-deficient
T16391 14324-14329 NN denotes cells
T16392 14330-14331 -LRB- denotes (
T16393 14331-14338 JJ denotes HOIP-/-
T16394 14339-14340 SYM denotes +
T16395 14341-14345 NN denotes pMIP
T16396 14345-14346 -RRB- denotes )
T16397 14346-14347 -COMMA- denotes ,
T16398 14348-14351 CC denotes but
T16399 14352-14358 JJ denotes intact
T16400 14359-14361 IN denotes in
T16401 14362-14370 JJ denotes parental
T16402 14371-14376 NN denotes cells
T16403 14377-14387 VB denotes transduced
T16404 14388-14392 IN denotes with
T16405 14393-14395 DT denotes an
T16406 14396-14401 JJ denotes empty
T16407 14402-14412 JJ denotes retroviral
T16408 14413-14419 NN denotes vector
T16409 14420-14421 -LRB- denotes (
T16410 14421-14427 NN denotes A20.2J
T16411 14428-14429 SYM denotes +
T16412 14430-14434 NN denotes pMIP
T16413 14434-14435 -RRB- denotes )
T16414 14436-14439 CC denotes and
T16415 14440-14442 IN denotes in
T16416 14443-14457 JJ denotes HOIP-deficient
T16417 14458-14463 NN denotes cells
T16418 14464-14474 VB denotes transduced
T16419 14475-14479 IN denotes with
T16420 14480-14481 DT denotes a
T16421 14482-14492 JJ denotes retroviral
T16422 14493-14499 NN denotes vector
T16423 14500-14508 VB denotes encoding
T16424 14509-14513 NN denotes HOIP
T16425 14514-14515 -LRB- denotes (
T16426 14515-14522 JJ denotes HOIP-/-
T16427 14523-14524 SYM denotes +
T16428 14525-14529 NN denotes HOIP
T16429 14529-14530 -RRB- denotes )
T16430 14532-14537 NN denotes Cells
T16431 14538-14542 VB denotes were
T16432 14543-14552 VB denotes activated
T16433 14553-14557 IN denotes with
T16434 14558-14574 JJ denotes CD154-expressing
T16435 14575-14581 NN denotes insect
T16436 14582-14587 NN denotes cells
T16437 14588-14591 IN denotes for
T16438 14592-14595 DT denotes the
T16439 14596-14601 NN denotes times
T16440 14602-14611 VB denotes indicated
T16441 14613-14615 IN denotes As
T16442 14616-14617 DT denotes a
T16443 14618-14626 JJ denotes negative
T16444 14627-14634 NN denotes control
T16445 14634-14635 -COMMA- denotes ,
T16446 14636-14641 NN denotes cells
T16447 14642-14646 VB denotes were
T16448 14647-14657 VB denotes stimulated
T16449 14658-14662 IN denotes with
T16450 14663-14669 NN denotes insect
T16451 14670-14675 NN denotes cells
T16452 14676-14683 VB denotes lacking
T16453 14684-14689 NN denotes CD154
T16454 14690-14691 -LRB- denotes (
T16455 14691-14692 CD denotes 5
T16456 14693-14699 JJ denotes minute
T16457 14700-14704 NN denotes time
T16458 14705-14710 NN denotes point
T16459 14711-14715 RB denotes only
T16460 14715-14716 -RRB- denotes )
T16461 14718-14725 JJ denotes Western
T16462 14726-14731 NN denotes blots
T16463 14732-14734 IN denotes of
T16464 14735-14745 JJ denotes whole-cell
T16465 14746-14753 NN denotes lysates
T16466 14754-14758 VB denotes were
T16467 14759-14765 VB denotes probed
T16468 14766-14770 IN denotes with
T16469 14771-14774 DT denotes the
T16470 14775-14784 JJ denotes indicated
T16471 14785-14795 NN denotes antibodies
T16472 14797-14809 NN denotes Phospho-IκBα
T16473 14810-14811 -LRB- denotes (
T16474 14811-14816 NN denotes pIκBα
T16475 14816-14817 -RRB- denotes )
T16476 14818-14821 CC denotes and
T16477 14822-14833 NN denotes phospho-JNK
T16478 14834-14835 -LRB- denotes (
T16479 14835-14839 NN denotes pJNK
T16480 14839-14840 -RRB- denotes )
T16481 14841-14846 NN denotes blots
T16482 14847-14851 VB denotes were
T16483 14852-14860 VB denotes stripped
T16484 14861-14864 CC denotes and
T16485 14865-14873 VB denotes reprobed
T16486 14874-14877 IN denotes for
T16487 14878-14883 JJ denotes total
T16488 14884-14888 NN denotes IκBα
T16489 14889-14892 CC denotes and
T16490 14893-14898 JJ denotes total
T16491 14899-14902 NN denotes JNK
T16492 14902-14903 -COMMA- denotes ,
T16493 14904-14916 RB denotes respectively
T16494 14917-14918 -LRB- denotes (
T16495 14918-14926 JJ denotes anti-JNK
T16496 14927-14937 NN denotes antibodies
T16497 14938-14947 VB denotes recognize
T16498 14948-14951 NN denotes p46
T16499 14952-14955 CC denotes and
T16500 14956-14959 NN denotes p54
T16501 14960-14968 NN denotes isoforms
T16502 14968-14969 -RRB- denotes )
T16503 14971-14975 NN denotes IκBα
T16504 14976-14981 NN denotes blots
T16505 14982-14986 VB denotes were
T16506 14987-14991 RB denotes also
T16507 14992-15000 VB denotes reprobed
T16508 15001-15004 IN denotes for
T16509 15005-15010 NN denotes actin
T16510 15011-15013 TO denotes to
T16511 15014-15025 VB denotes demonstrate
T16512 15026-15031 JJ denotes equal
T16513 15032-15036 NN denotes lane
T16514 15037-15044 NN denotes loading
T16515 15046-15055 JJ denotes Molecular
T16516 15056-15063 NN denotes weights
T16517 15064-15067 VB denotes are
T16518 15068-15077 VB denotes indicated
T16519 15078-15080 IN denotes at
T16520 15081-15086 NN denotes right
T16521 15088-15095 JJ denotes Similar
T16522 15096-15103 NN denotes results
T16523 15104-15108 VB denotes were
T16524 15109-15117 VB denotes obtained
T16525 15118-15120 IN denotes in
T16526 15121-15122 DT denotes a
T16527 15123-15129 JJ denotes second
T16528 15130-15140 NN denotes experiment
T16529 15141-15144 CC denotes and
T16530 15145-15147 IN denotes in
T16531 15148-15151 CD denotes two
T16532 15152-15162 JJ denotes additional
T16533 15163-15174 NN denotes experiments
T16534 15175-15179 IN denotes with
T16535 15180-15181 DT denotes a
T16536 15182-15188 JJ denotes second
T16537 15189-15203 JJ denotes HOIP-deficient
T16538 15204-15209 NN denotes clone
T7484 15265-15269 IN denotes with
T7485 15270-15274 NN denotes CD40
T7486 15275-15278 DT denotes The
T7487 15279-15285 JJ denotes marked
T7488 15286-15293 NN denotes defects
T7489 15294-15296 IN denotes in
T7490 15297-15310 JJ denotes CD40-mediated
T7491 15311-15315 NN denotes cell
T7492 15316-15326 NN denotes activation
T7493 15327-15330 CC denotes and
T7494 15331-15340 NN denotes signaling
T7495 15341-15350 VB denotes displayed
T7496 15351-15353 IN denotes by
T7497 15354-15368 JJ denotes HOIP-deficient
T7498 15369-15374 NN denotes cells
T7499 15375-15384 VB denotes suggested
T7500 15385-15389 IN denotes that
T7501 15390-15394 NN denotes HOIP
T7502 15395-15403 VB denotes mediates
T7503 15404-15415 NN denotes recruitment
T7504 15416-15418 IN denotes of
T7505 15419-15427 JJ denotes critical
T7506 15428-15438 NN denotes components
T7507 15439-15441 IN denotes of
T7508 15442-15445 DT denotes the
T7509 15446-15450 NN denotes CD40
T7510 15451-15460 NN denotes signaling
T7511 15461-15470 NN denotes apparatus
T7512 15471-15473 TO denotes to
T7513 15474-15477 DT denotes the
T7514 15478-15486 NN denotes receptor
T7515 15488-15498 RB denotes Previously
T7516 15498-15499 -COMMA- denotes ,
T7517 15500-15502 PRP denotes we
T7518 15503-15515 VB denotes demonstrated
T7519 15516-15520 IN denotes that
T7520 15521-15525 NN denotes HOIP
T7521 15526-15528 VB denotes is
T7522 15529-15538 VB denotes recruited
T7523 15539-15541 TO denotes to
T7524 15542-15545 DT denotes the
T7525 15546-15550 NN denotes CD40
T7526 15551-15560 NN denotes signaling
T7527 15561-15568 NN denotes complex
T7528 15569-15571 IN denotes in
T7529 15572-15573 DT denotes a
T7530 15574-15589 JJ denotes TRAF2-dependent
T7531 15590-15596 NN denotes manner
T7532 15596-15597 -COMMA- denotes ,
T7533 15598-15608 VB denotes suggesting
T7534 15609-15613 IN denotes that
T7535 15614-15618 NN denotes HOIP
T7536 15619-15628 NN denotes functions
T7537 15629-15639 JJ denotes downstream
T7538 15640-15642 IN denotes of
T7539 15643-15648 NN denotes TRAF2
T7540 15649-15650 -LRB- denotes [
T7541 15650-15651 CD denotes 6
T7542 15651-15652 -RRB- denotes ]
T7543 15654-15661 NN denotes Studies
T7544 15662-15664 IN denotes by
T7545 15665-15671 NN denotes others
T7546 15672-15679 VB denotes suggest
T7547 15680-15684 IN denotes that
T7548 15685-15688 DT denotes the
T7549 15689-15705 JJ denotes TRAF2-associated
T7550 15706-15714 NN denotes proteins
T7551 15715-15720 NN denotes cIAP1
T7552 15721-15724 CC denotes and
T7553 15725-15730 NN denotes cIAP2
T7554 15731-15735 VB denotes play
T7555 15736-15737 DT denotes a
T7556 15738-15742 NN denotes role
T7557 15743-15745 IN denotes in
T7558 15746-15749 DT denotes the
T7559 15750-15761 NN denotes recruitment
T7560 15762-15764 IN denotes of
T7561 15765-15769 NN denotes HOIP
T7562 15770-15772 TO denotes to
T7563 15773-15778 NN denotes TNFR1
T7564 15779-15782 CC denotes and
T7565 15783-15787 NN denotes CD40
T7566 15788-15789 -LRB- denotes [
T7567 15789-15791 CD denotes 14
T7568 15791-15792 -RRB- denotes ]
T7569 15794-15803 RB denotes Therefore
T7570 15803-15804 -COMMA- denotes ,
T7571 15805-15807 PRP denotes we
T7572 15808-15818 VB denotes determined
T7573 15819-15826 IN denotes whether
T7574 15827-15830 DT denotes the
T7575 15831-15842 NN denotes association
T7576 15843-15845 IN denotes of
T7577 15846-15850 NN denotes HOIP
T7578 15851-15855 IN denotes with
T7579 15856-15860 NN denotes CD40
T7580 15861-15863 IN denotes in
T7581 15864-15870 NN denotes A20.2J
T7582 15871-15876 NN denotes cells
T7583 15877-15880 VB denotes was
T7584 15881-15888 VB denotes altered
T7585 15889-15891 IN denotes by
T7586 15892-15901 NN denotes treatment
T7587 15902-15904 IN denotes of
T7588 15905-15910 NN denotes cells
T7589 15911-15915 IN denotes with
T7590 15916-15918 DT denotes an
T7591 15919-15928 NN denotes inhibitor
T7592 15929-15931 IN denotes of
T7593 15932-15936 NN denotes cIAP
T7594 15937-15945 NN denotes activity
T7595 15945-15946 -COMMA- denotes ,
T7596 15947-15948 DT denotes a
T7597 15949-15967 JJ denotes membrane-permeable
T7598 15968-15975 NN denotes peptide
T7599 15976-15983 VB denotes derived
T7600 15984-15988 IN denotes from
T7601 15989-15992 DT denotes the
T7602 15993-16002 NN denotes apoptosis
T7603 16003-16012 NN denotes regulator
T7604 16013-16017 NN denotes SMAC
T7605 16018-16019 -LRB- denotes [
T7606 16019-16021 CD denotes 15
T7607 16021-16022 -RRB- denotes ]
T7608 16024-16026 PRP denotes We
T7609 16027-16032 VB denotes found
T7610 16033-16037 IN denotes that
T7611 16038-16050 NN denotes pretreatment
T7612 16051-16053 IN denotes of
T7613 16054-16059 NN denotes cells
T7614 16060-16064 IN denotes with
T7615 16065-16068 DT denotes the
T7616 16069-16073 NN denotes SMAC
T7617 16074-16081 NN denotes peptide
T7618 16082-16094 RB denotes dramatically
T7619 16095-16102 VB denotes reduced
T7620 16103-16106 DT denotes the
T7621 16107-16113 NN denotes amount
T7622 16114-16116 IN denotes of
T7623 16117-16122 NN denotes cIAP1
T7624 16123-16133 VB denotes associated
T7625 16134-16138 IN denotes with
T7626 16139-16142 DT denotes the
T7627 16143-16147 NN denotes CD40
T7628 16148-16157 NN denotes signaling
T7629 16158-16165 NN denotes complex
T7630 16166-16168 IN denotes in
T7631 16169-16174 NN denotes cells
T7632 16175-16185 VB denotes stimulated
T7633 16186-16190 IN denotes with
T7634 16191-16200 JJ denotes anti-CD40
T7635 16201-16216 JJ denotes antibody-coated
T7636 16217-16222 NN denotes beads
T7637 16223-16224 -LRB- denotes (
T7638 16224-16228 NNP denotes Fig.
T7639 16229-16231 NN denotes 5A
T7640 16231-16232 -RRB- denotes )
T7641 16234-16238 NN denotes SMAC
T7642 16239-16246 NN denotes peptide
T7643 16247-16256 NN denotes treatment
T7644 16257-16261 RB denotes also
T7645 16262-16270 VB denotes resulted
T7646 16271-16273 IN denotes in
T7647 16274-16275 DT denotes a
T7648 16276-16282 JJ denotes slight
T7649 16283-16286 CC denotes but
T7650 16287-16299 JJ denotes reproducible
T7651 16300-16308 NN denotes decrease
T7652 16309-16311 IN denotes in
T7653 16312-16315 DT denotes the
T7654 16316-16322 NN denotes amount
T7655 16323-16325 IN denotes of
T7656 16326-16329 DT denotes the
T7657 16330-16335 JJ denotes major
T7658 16336-16340 NN denotes HOIP
T7659 16341-16345 NN denotes form
T7660 16346-16355 VB denotes recovered
T7661 16356-16358 IN denotes by
T7662 16359-16363 NN denotes CD40
T7663 16364-16383 NN denotes immunoprecipitation
T7664 16383-16384 -COMMA- denotes ,
T7665 16385-16390 IN denotes along
T7666 16391-16395 IN denotes with
T7667 16396-16398 DT denotes an
T7668 16399-16407 JJ denotes apparent
T7669 16408-16416 NN denotes increase
T7670 16417-16419 IN denotes in
T7671 16420-16426 JJ denotes higher
T7672 16427-16436 JJ denotes molecular
T7673 16437-16443 NN denotes weight
T7674 16444-16451 NN denotes species
T7675 16452-16462 VB denotes recognized
T7676 16463-16465 IN denotes by
T7677 16466-16475 JJ denotes anti-HOIP
T7678 16476-16484 NN denotes antibody
T7679 16486-16488 IN denotes In
T7680 16489-16497 NN denotes contrast
T7681 16497-16498 -COMMA- denotes ,
T7682 16499-16508 NN denotes treatment
T7683 16509-16513 IN denotes with
T7684 16514-16517 DT denotes the
T7685 16518-16522 NN denotes SMAC
T7686 16523-16530 NN denotes peptide
T7687 16531-16534 VB denotes did
T7688 16535-16538 RB denotes not
T7689 16539-16544 VB denotes alter
T7690 16545-16548 DT denotes the
T7691 16549-16555 NN denotes amount
T7692 16556-16558 CC denotes or
T7693 16559-16568 JJ denotes molecular
T7694 16569-16575 NN denotes weight
T7695 16576-16578 IN denotes of
T7696 16579-16583 NN denotes HOIP
T7697 16584-16591 JJ denotes present
T7698 16592-16594 IN denotes in
T7699 16595-16599 NN denotes cell
T7700 16600-16607 NN denotes lysates
T7701 16609-16614 DT denotes These
T7702 16615-16619 NN denotes data
T7703 16620-16627 VB denotes suggest
T7704 16628-16632 IN denotes that
T7705 16633-16637 NN denotes SMAC
T7706 16638-16645 NN denotes peptide
T7707 16646-16655 NN denotes treatment
T7708 16656-16668 RB denotes specifically
T7709 16669-16675 VB denotes alters
T7710 16676-16679 DT denotes the
T7711 16680-16695 NN denotes characteristics
T7712 16696-16698 IN denotes of
T7713 16699-16714 JJ denotes CD40-associated
T7714 16715-16719 NN denotes HOIP
T7715 16720-16726 RB denotes rather
T7716 16727-16731 IN denotes than
T7717 16732-16735 DT denotes the
T7718 16736-16742 JJ denotes entire
T7719 16743-16751 JJ denotes cellular
T7720 16752-16756 NN denotes pool
T7721 16757-16759 IN denotes of
T7722 16760-16764 DT denotes this
T7723 16765-16772 NN denotes protein
T7724 16774-16782 RB denotes Together
T7725 16782-16783 -COMMA- denotes ,
T7726 16784-16789 DT denotes these
T7727 16790-16797 NN denotes results
T7728 16798-16805 VB denotes support
T7729 16806-16809 DT denotes the
T7730 16810-16814 NN denotes idea
T7731 16815-16819 IN denotes that
T7732 16820-16823 DT denotes the
T7733 16824-16828 NN denotes cIAP
T7734 16829-16837 NN denotes proteins
T7735 16838-16847 VB denotes influence
T7736 16848-16851 DT denotes the
T7737 16852-16863 NN denotes recruitment
T7738 16864-16867 CC denotes and
T7739 16868-16886 JJ denotes post-translational
T7740 16887-16899 NN denotes modification
T7741 16900-16905 NN denotes state
T7742 16906-16908 IN denotes of
T7743 16909-16924 JJ denotes CD40-associated
T7744 16925-16929 NN denotes HOIP
T17281 16975-16978 NN denotes IKK
T17282 16979-16990 NN denotes recruitment
T17283 16991-16993 TO denotes to
T17284 16994-16997 DT denotes the
T17285 16998-17002 NN denotes CD40
T17286 17003-17012 NN denotes signaling
T17287 17013-17020 NN denotes complex
T17288 17021-17023 VB denotes is
T17289 17024-17033 JJ denotes defective
T17290 17034-17036 IN denotes in
T17291 17037-17051 JJ denotes HOIP-deficient
T17292 17052-17057 NN denotes cells
T17293 17059-17060 -LRB- denotes (
T17294 17060-17061 NN denotes A
T17295 17061-17062 -RRB- denotes )
T17296 17063-17067 NN denotes SMAC
T17297 17068-17075 NN denotes peptide
T17298 17076-17085 NN denotes treatment
T17299 17086-17093 VB denotes reduces
T17300 17094-17105 NN denotes recruitment
T17301 17106-17108 IN denotes of
T17302 17109-17114 NN denotes cIAP1
T17303 17115-17117 TO denotes to
T17304 17118-17122 NN denotes CD40
T17305 17123-17126 CC denotes and
T17306 17127-17130 MD denotes may
T17307 17131-17137 VB denotes modify
T17308 17138-17142 NN denotes HOIP
T17309 17143-17154 NN denotes recruitment
T17310 17156-17162 NN denotes A20.2J
T17311 17163-17168 NN denotes cells
T17312 17169-17173 VB denotes were
T17313 17174-17183 VB denotes incubated
T17314 17184-17187 IN denotes for
T17315 17188-17191 CD denotes six
T17316 17192-17197 NN denotes hours
T17317 17198-17202 IN denotes with
T17318 17203-17221 JJ denotes membrane-permeable
T17319 17222-17229 NN denotes SMAC-N7
T17320 17230-17237 NN denotes peptide
T17321 17238-17240 CC denotes or
T17322 17241-17244 CD denotes 1.5
T17323 17244-17245 NN denotes %
T17324 17246-17250 NN denotes DMSO
T17325 17251-17252 -LRB- denotes (
T17326 17252-17259 NN denotes solvent
T17327 17260-17264 VB denotes used
T17328 17265-17268 IN denotes for
T17329 17269-17272 DT denotes the
T17330 17273-17280 NN denotes peptide
T17331 17280-17281 -RRB- denotes )
T17332 17283-17292 VB denotes Following
T17333 17293-17296 DT denotes the
T17334 17297-17307 NN denotes incubation
T17335 17307-17308 -COMMA- denotes ,
T17336 17309-17313 NN denotes cell
T17337 17314-17321 NN denotes lysates
T17338 17322-17326 VB denotes were
T17339 17327-17335 VB denotes prepared
T17340 17335-17336 -COMMA- denotes ,
T17341 17337-17349 VB denotes fractionated
T17342 17350-17352 IN denotes by
T17343 17353-17361 NN denotes SDS-PAGE
T17344 17361-17362 -COMMA- denotes ,
T17345 17363-17366 CC denotes and
T17346 17367-17376 VB denotes evaluated
T17347 17377-17379 IN denotes by
T17348 17380-17387 JJ denotes Western
T17349 17388-17392 NN denotes blot
T17350 17393-17394 -LRB- denotes (
T17351 17394-17399 NN denotes lanes
T17352 17400-17401 CD denotes 1
T17353 17402-17405 CC denotes and
T17354 17406-17407 CD denotes 2
T17355 17407-17408 -RRB- denotes )
T17356 17410-17415 NN denotes Cells
T17357 17416-17425 VB denotes incubated
T17358 17426-17430 IN denotes with
T17359 17431-17435 NN denotes DMSO
T17360 17436-17438 CC denotes or
T17361 17439-17446 NN denotes SMAC-N7
T17362 17447-17451 VB denotes were
T17363 17452-17456 RB denotes also
T17364 17457-17467 VB denotes stimulated
T17365 17468-17472 IN denotes with
T17366 17473-17481 JJ denotes magnetic
T17367 17482-17487 NN denotes beads
T17368 17488-17494 VB denotes coated
T17369 17495-17499 IN denotes with
T17370 17500-17509 NN denotes anti-CD40
T17371 17510-17512 CC denotes or
T17372 17513-17515 DT denotes an
T17373 17516-17523 NN denotes isotype
T17374 17524-17531 NN denotes control
T17375 17532-17540 NN denotes antibody
T17376 17542-17560 VB denotes Immunoprecipitated
T17377 17561-17562 -LRB- denotes (
T17378 17562-17564 NN denotes IP
T17379 17564-17565 -RRB- denotes )
T17380 17566-17574 NN denotes material
T17381 17575-17580 VB denotes bound
T17382 17581-17583 TO denotes to
T17383 17584-17587 DT denotes the
T17384 17588-17593 NN denotes beads
T17385 17594-17597 VB denotes was
T17386 17598-17604 VB denotes loaded
T17387 17605-17607 IN denotes in
T17388 17608-17613 NN denotes lanes
T17389 17614-17617 CD denotes 3-5
T17390 17619-17626 NN denotes Samples
T17391 17627-17629 IN denotes of
T17392 17630-17633 DT denotes the
T17393 17634-17638 NN denotes cell
T17394 17639-17646 NN denotes lysates
T17395 17647-17652 IN denotes after
T17396 17653-17672 NN denotes immunoprecipitation
T17397 17673-17679 VB denotes appear
T17398 17680-17682 IN denotes in
T17399 17683-17688 NN denotes lanes
T17400 17689-17692 CD denotes 6–8
T17401 17694-17701 JJ denotes Western
T17402 17702-17707 NN denotes blots
T17403 17708-17712 VB denotes were
T17404 17713-17719 VB denotes probed
T17405 17720-17724 IN denotes with
T17406 17725-17735 NN denotes antibodies
T17407 17736-17744 JJ denotes specific
T17408 17745-17748 IN denotes for
T17409 17749-17754 NN denotes cIAP1
T17410 17754-17755 -COMMA- denotes ,
T17411 17756-17761 NN denotes TRAF2
T17412 17761-17762 -COMMA- denotes ,
T17413 17763-17768 NN denotes TRAF3
T17414 17768-17769 -COMMA- denotes ,
T17415 17770-17773 CC denotes and
T17416 17774-17778 NN denotes HOIP
T17417 17779-17780 -LRB- denotes (
T17418 17780-17791 JJ denotes approximate
T17419 17792-17801 JJ denotes molecular
T17420 17802-17809 NN denotes weights
T17421 17810-17819 VB denotes indicated
T17422 17820-17822 IN denotes on
T17423 17823-17827 JJ denotes left
T17424 17827-17828 -RRB- denotes )
T17425 17830-17837 JJ denotes Similar
T17426 17838-17845 NN denotes results
T17427 17846-17850 VB denotes were
T17428 17851-17859 VB denotes obtained
T17429 17860-17862 IN denotes in
T17430 17863-17866 CD denotes two
T17431 17867-17877 JJ denotes additional
T17432 17878-17890 NN denotes experiments.
T17433 17891-17892 -LRB- denotes (
T17434 17892-17893 NN denotes B
T17435 17893-17894 -RRB- denotes )
T17436 17895-17899 NN denotes CD40
T17437 17900-17903 VB denotes was
T17438 17904-17912 VB denotes isolated
T17439 17913-17915 IN denotes by
T17440 17916-17935 NN denotes immunoprecipitation
T17441 17936-17937 -LRB- denotes (
T17442 17937-17939 IN denotes as
T17443 17940-17942 IN denotes in
T17444 17943-17944 -LRB- denotes (
T17445 17944-17945 NN denotes A
T17446 17945-17946 -RRB- denotes )
T17447 17946-17947 -RRB- denotes )
T17448 17948-17952 IN denotes from
T17449 17953-17959 NN denotes A20.2J
T17450 17960-17965 NN denotes cells
T17451 17966-17969 CC denotes and
T17452 17970-17984 JJ denotes HOIP-deficient
T17453 17985-17990 NN denotes cells
T17454 17991-18001 VB denotes transduced
T17455 18002-18006 IN denotes with
T17456 18007-18009 DT denotes an
T17457 18010-18015 JJ denotes empty
T17458 18016-18026 JJ denotes retroviral
T17459 18027-18033 NN denotes vector
T17460 18034-18035 -LRB- denotes (
T17461 18035-18039 NN denotes pMIP
T17462 18039-18040 -RRB- denotes )
T17463 18041-18043 CC denotes or
T17464 18044-18045 DT denotes a
T17465 18046-18056 JJ denotes retroviral
T17466 18057-18063 NN denotes vector
T17467 18064-18072 VB denotes encoding
T17468 18073-18077 NN denotes HOIP
T17469 18079-18087 NN denotes Material
T17470 18088-18106 VB denotes immunoprecipitated
T17471 18107-18111 IN denotes with
T17472 18112-18114 DT denotes an
T17473 18115-18122 NN denotes isotype
T17474 18123-18130 NN denotes control
T17475 18131-18139 NN denotes antibody
T17476 18140-18141 -LRB- denotes (
T17477 18141-18148 NN denotes isotype
T17478 18148-18149 -RRB- denotes )
T17479 18150-18152 CC denotes or
T17480 18153-18162 JJ denotes anti-CD40
T17481 18163-18171 NN denotes antibody
T17482 18172-18175 VB denotes was
T17483 18176-18184 VB denotes examined
T17484 18185-18187 IN denotes by
T17485 18188-18195 JJ denotes Western
T17486 18196-18204 NN denotes blotting
T17487 18205-18208 IN denotes for
T17488 18209-18213 NN denotes CD40
T17489 18213-18214 -COMMA- denotes ,
T17490 18215-18220 NN denotes TRAF2
T17491 18220-18221 -COMMA- denotes ,
T17492 18222-18227 NN denotes TRAF3
T17493 18227-18228 -COMMA- denotes ,
T17494 18229-18234 NN denotes cIAP1
T17495 18234-18235 -COMMA- denotes ,
T17496 18236-18240 NN denotes HOIP
T17497 18240-18241 -COMMA- denotes ,
T17498 18242-18248 NN denotes IKKα/β
T17499 18248-18249 -COMMA- denotes ,
T17500 18250-18253 CC denotes and
T17501 18254-18258 NN denotes IKKγ
T17502 18259-18260 -LRB- denotes (
T17503 18260-18265 JJ denotes right
T17504 18266-18272 NN denotes panels
T17505 18272-18273 -RRB- denotes )
T17506 18275-18279 NN denotes HOIP
T17507 18280-18290 NN denotes expression
T17508 18291-18294 VB denotes was
T17509 18295-18303 VB denotes required
T17510 18304-18307 IN denotes for
T17511 18308-18323 NN denotes coprecipitation
T17512 18324-18326 IN denotes of
T17513 18327-18330 NN denotes IKK
T17514 18331-18339 NN denotes proteins
T17515 18340-18344 IN denotes with
T17516 18345-18349 NN denotes CD40
T17517 18351-18355 NN denotes Cell
T17518 18356-18363 NN denotes lysates
T17519 18364-18368 IN denotes from
T17520 18369-18381 JJ denotes unstimulated
T17521 18382-18387 NN denotes cells
T17522 18388-18391 VB denotes are
T17523 18392-18397 VB denotes shown
T17524 18398-18400 IN denotes in
T17525 18401-18404 DT denotes the
T17526 18405-18409 JJ denotes left
T17527 18410-18416 NN denotes panels
T17528 18418-18425 JJ denotes Similar
T17529 18426-18433 NN denotes results
T17530 18434-18438 VB denotes were
T17531 18439-18447 VB denotes obtained
T17532 18448-18450 IN denotes in
T17533 18451-18452 DT denotes a
T17534 18453-18459 JJ denotes second
T17535 18460-18470 NN denotes experiment
T17536 18471-18474 CC denotes and
T17537 18475-18477 IN denotes in
T17538 18478-18481 CD denotes two
T17539 18482-18493 NN denotes experiments
T17540 18494-18498 IN denotes with
T17541 18499-18500 DT denotes a
T17542 18501-18507 JJ denotes second
T17543 18508-18522 JJ denotes HOIP-deficient
T17544 18523-18529 NN denotes clone.
T17545 18530-18531 -LRB- denotes (
T17546 18531-18532 NN denotes C
T17547 18532-18533 -RRB- denotes )
T17548 18534-18536 TO denotes To
T17549 18537-18544 RB denotes further
T17550 18545-18553 VB denotes evaluate
T17551 18554-18568 JJ denotes HOIP-dependent
T17552 18569-18580 NN denotes recruitment
T17553 18581-18583 IN denotes of
T17554 18584-18588 NN denotes IKKγ
T17555 18589-18591 TO denotes to
T17556 18592-18596 NN denotes CD40
T17557 18596-18597 -COMMA- denotes ,
T17558 18598-18604 NN denotes A20.2J
T17559 18605-18610 NN denotes cells
T17560 18611-18613 CC denotes or
T17561 18614-18628 JJ denotes HOIP-deficient
T17562 18629-18630 -LRB- denotes (
T17563 18630-18637 JJ denotes HOIP-/-
T17564 18637-18638 -RRB- denotes )
T17565 18639-18645 NN denotes A20.2J
T17566 18646-18651 NN denotes cells
T17567 18652-18656 VB denotes were
T17568 18657-18667 VB denotes transduced
T17569 18668-18672 IN denotes with
T17570 18673-18675 DT denotes an
T17571 18676-18681 JJ denotes empty
T17572 18682-18692 JJ denotes retroviral
T17573 18693-18699 NN denotes vector
T17574 18700-18702 CC denotes or
T17575 18703-18704 DT denotes a
T17576 18705-18715 JJ denotes retroviral
T17577 18716-18725 NN denotes construct
T17578 18726-18734 VB denotes encoding
T17579 18735-18737 NN denotes BP
T17580 18738-18752 JJ denotes epitope-tagged
T17581 18753-18757 NN denotes IKKγ
T17582 18758-18759 -LRB- denotes (
T17583 18759-18764 VB denotes noted
T17584 18765-18767 IN denotes in
T17585 18768-18771 DT denotes the
T17586 18772-18778 NN denotes figure
T17587 18779-18781 IN denotes as
T17588 18782-18786 NN denotes pMIP
T17589 18787-18790 CC denotes and
T17590 18791-18795 NN denotes IKKγ
T17591 18795-18796 -COMMA- denotes ,
T17592 18797-18809 RB denotes respectively
T17593 18809-18810 -RRB- denotes )
T17594 18812-18819 NN denotes Lysates
T17595 18820-18821 -LRB- denotes (
T17596 18821-18826 NN denotes lanes
T17597 18827-18830 CD denotes 1–3
T17598 18830-18831 -RRB- denotes )
T17599 18832-18835 CC denotes and
T17600 18836-18855 NN denotes immunoprecipitation
T17601 18856-18857 -LRB- denotes (
T17602 18857-18859 NN denotes IP
T17603 18859-18860 -RRB- denotes )
T17604 18861-18868 NN denotes samples
T17605 18869-18870 -LRB- denotes (
T17606 18870-18875 NN denotes lanes
T17607 18876-18880 CD denotes 4–11
T17608 18880-18881 -RRB- denotes )
T17609 18882-18886 IN denotes from
T17610 18887-18890 DT denotes the
T17611 18891-18895 NN denotes cell
T17612 18896-18901 NN denotes lines
T17613 18902-18906 VB denotes were
T17614 18907-18919 VB denotes fractionated
T17615 18920-18922 IN denotes by
T17616 18923-18931 NN denotes SDS-PAGE
T17617 18932-18935 CC denotes and
T17618 18936-18945 VB denotes evaluated
T17619 18946-18948 IN denotes by
T17620 18949-18956 JJ denotes Western
T17621 18957-18965 NN denotes blotting
T17622 18966-18970 IN denotes with
T17623 18971-18981 NN denotes antibodies
T17624 18982-18984 TO denotes to
T17625 18985-18988 DT denotes the
T17626 18989-18991 NN denotes BP
T17627 18992-18995 NN denotes tag
T17628 18996-18997 -LRB- denotes (
T17629 18997-19001 NN denotes IKKγ
T17630 19001-19002 -COMMA- denotes ,
T17631 19003-19008 JJ denotes upper
T17632 19009-19014 NN denotes panel
T17633 19014-19015 -RRB- denotes )
T17634 19016-19019 CC denotes and
T17635 19020-19025 NN denotes TRAF2
T17636 19027-19030 DT denotes The
T17637 19031-19040 JJ denotes anti-CD40
T17638 19041-19043 NN denotes IP
T17639 19044-19050 NN denotes sample
T17640 19051-19053 IN denotes in
T17641 19054-19058 NN denotes lane
T17642 19059-19061 CD denotes 11
T17643 19062-19065 VB denotes was
T17644 19066-19073 VB denotes treated
T17645 19074-19078 IN denotes with
T17646 19079-19080 NN denotes λ
T17647 19081-19092 NN denotes phosphatase
T17648 19092-19093 -COLON- denotes ;
T17649 19094-19097 DT denotes the
T17650 19098-19104 NN denotes sample
T17651 19105-19107 IN denotes in
T17652 19108-19112 NN denotes lane
T17653 19113-19115 CD denotes 10
T17654 19116-19119 VB denotes was
T17655 19120-19132 JJ denotes mock-treated
T17656 19134-19141 NN denotes Protein
T17657 19142-19149 NN denotes samples
T17658 19150-19151 -LRB- denotes (
T17659 19151-19156 CC denotes minus
T17660 19157-19162 DT denotes those
T17661 19163-19170 VB denotes treated
T17662 19171-19175 IN denotes with
T17663 19176-19187 NN denotes phosphatase
T17664 19187-19188 -RRB- denotes )
T17665 19189-19193 VB denotes were
T17666 19194-19198 RB denotes also
T17667 19199-19211 VB denotes fractionated
T17668 19212-19214 IN denotes on
T17669 19215-19216 DT denotes a
T17670 19217-19225 JJ denotes separate
T17671 19226-19229 NN denotes gel
T17672 19230-19231 -LRB- denotes (
T17673 19231-19236 JJ denotes lower
T17674 19237-19247 NN denotes acrylamide
T17675 19248-19261 NN denotes concentration
T17676 19261-19262 -RRB- denotes )
T17677 19263-19266 IN denotes for
T17678 19267-19270 DT denotes the
T17679 19271-19281 NN denotes evaluation
T17680 19282-19284 IN denotes of
T17681 19285-19290 NN denotes TRAF3
T17682 19291-19294 CC denotes and
T17683 19295-19299 NN denotes HOIP
T17684 19300-19301 -LRB- denotes (
T17685 19301-19307 NN denotes bottom
T17686 19308-19311 CD denotes two
T17687 19312-19318 NN denotes panels
T17688 19318-19319 -RRB- denotes )
T17689 19321-19328 JJ denotes Similar
T17690 19329-19336 NN denotes results
T17691 19337-19341 VB denotes were
T17692 19342-19350 VB denotes obtained
T17693 19351-19353 IN denotes in
T17694 19354-19357 CD denotes two
T17695 19358-19368 JJ denotes additional
T17696 19369-19380 NN denotes experiments
T7745 19384-19386 TO denotes To
T7746 19387-19391 VB denotes test
T7747 19392-19395 DT denotes the
T7748 19396-19407 NN denotes possibility
T7749 19408-19412 IN denotes that
T7750 19413-19417 NN denotes HOIP
T7751 19418-19420 VB denotes is
T7752 19421-19432 JJ denotes responsible
T7753 19433-19436 IN denotes for
T7754 19437-19440 DT denotes the
T7755 19441-19452 NN denotes recruitment
T7756 19453-19455 IN denotes of
T7757 19456-19461 JJ denotes other
T7758 19462-19470 JJ denotes critical
T7759 19471-19480 NN denotes signaling
T7760 19481-19489 NN denotes proteins
T7761 19490-19492 TO denotes to
T7762 19493-19497 NN denotes CD40
T7763 19497-19498 -COMMA- denotes ,
T7764 19499-19501 PRP denotes we
T7765 19502-19520 VB denotes immunoprecipitated
T7766 19521-19525 NN denotes CD40
T7767 19526-19535 NN denotes signaling
T7768 19536-19545 NN denotes complexes
T7769 19546-19550 IN denotes from
T7770 19551-19565 JJ denotes HOIP-deficient
T7771 19566-19569 CC denotes and
T7772 19570-19588 JJ denotes HOIP-reconstituted
T7773 19589-19594 NN denotes cells
T7774 19595-19596 -LRB- denotes (
T7775 19596-19600 NN denotes Fig.
T7776 19601-19603 NN denotes 5B
T7777 19603-19604 -RRB- denotes )
T7778 19606-19608 IN denotes As
T7779 19609-19618 VB denotes described
T7780 19619-19629 RB denotes previously
T7781 19629-19630 -COMMA- denotes ,
T7782 19631-19635 NN denotes CD40
T7783 19636-19645 NN denotes signaling
T7784 19646-19655 NN denotes complexes
T7785 19656-19674 VB denotes immunoprecipitated
T7786 19675-19679 IN denotes from
T7787 19680-19686 NN denotes A20.2J
T7788 19687-19692 NN denotes cells
T7789 19693-19702 VB denotes contained
T7790 19703-19707 NN denotes HOIP
T7791 19708-19710 RB denotes as
T7792 19711-19715 RB denotes well
T7793 19716-19718 IN denotes as
T7794 19719-19724 NN denotes TRAF2
T7795 19724-19725 -COMMA- denotes ,
T7796 19726-19731 NN denotes TRAF3
T7797 19731-19732 -COMMA- denotes ,
T7798 19733-19739 NN denotes IKKα/β
T7799 19739-19740 -COMMA- denotes ,
T7800 19741-19744 CC denotes and
T7801 19745-19749 NNP denotes IKKγ
T7802 19750-19751 -LRB- denotes [
T7803 19751-19752 CD denotes 6
T7804 19752-19753 -RRB- denotes ]
T7805 19755-19758 DT denotes The
T7806 19759-19766 NN denotes amounts
T7807 19767-19769 IN denotes of
T7808 19770-19775 NN denotes TRAF2
T7809 19775-19776 -COMMA- denotes ,
T7810 19777-19782 NN denotes TRAF3
T7811 19782-19783 -COMMA- denotes ,
T7812 19784-19787 CC denotes and
T7813 19788-19793 NN denotes cIAP1
T7814 19794-19796 IN denotes in
T7815 19797-19801 NN denotes CD40
T7816 19802-19820 NN denotes immunoprecipitates
T7817 19821-19825 IN denotes from
T7818 19826-19840 JJ denotes HOIP-deficient
T7819 19841-19846 NN denotes cells
T7820 19847-19851 VB denotes were
T7821 19852-19859 JJ denotes similar
T7822 19860-19862 TO denotes to
T7823 19863-19868 DT denotes those
T7824 19869-19873 IN denotes from
T7825 19874-19882 JJ denotes parental
T7826 19883-19889 NN denotes A20.2J
T7827 19890-19895 NN denotes cells
T7828 19895-19896 -COMMA- denotes ,
T7829 19897-19907 VB denotes indicating
T7830 19908-19912 IN denotes that
T7831 19913-19917 NN denotes HOIP
T7832 19918-19920 VB denotes is
T7833 19921-19924 RB denotes not
T7834 19925-19933 VB denotes required
T7835 19934-19937 IN denotes for
T7836 19938-19941 DT denotes the
T7837 19942-19953 NN denotes association
T7838 19954-19956 IN denotes of
T7839 19957-19962 DT denotes these
T7840 19963-19971 NN denotes proteins
T7841 19972-19976 IN denotes with
T7842 19977-19981 NN denotes CD40
T7843 19983-19985 IN denotes In
T7844 19986-19994 NN denotes contrast
T7845 19994-19995 -COMMA- denotes ,
T7846 19996-20002 NN denotes IKKα/β
T7847 20003-20006 CC denotes and
T7848 20007-20011 NN denotes IKKγ
T7849 20012-20016 VB denotes were
T7850 20017-20020 RB denotes not
T7851 20021-20031 JJ denotes detectable
T7852 20032-20034 IN denotes in
T7853 20035-20039 NN denotes CD40
T7854 20040-20058 NN denotes immunoprecipitates
T7855 20059-20068 VB denotes recovered
T7856 20069-20073 IN denotes from
T7857 20074-20088 JJ denotes HOIP-deficient
T7858 20089-20094 NN denotes cells
T7859 20095-20096 -LRB- denotes (
T7860 20096-20100 NN denotes Fig.
T7861 20101-20103 NN denotes 5B
T7862 20103-20104 -COMMA- denotes ,
T7863 20104-20105 NN denotes C
T7864 20105-20106 -RRB- denotes )
T7865 20108-20111 DT denotes The
T7866 20112-20119 NN denotes amounts
T7867 20120-20122 IN denotes of
T7868 20123-20129 NN denotes IKKα/β
T7869 20130-20133 CC denotes and
T7870 20134-20138 NN denotes IKKγ
T7871 20139-20141 IN denotes in
T7872 20142-20146 NN denotes CD40
T7873 20147-20165 NN denotes immunoprecipitates
T7874 20166-20170 IN denotes from
T7875 20171-20189 JJ denotes HOIP-reconstituted
T7876 20190-20195 NN denotes cells
T7877 20196-20200 VB denotes were
T7878 20201-20208 JJ denotes similar
T7879 20209-20211 TO denotes to
T7880 20212-20217 DT denotes those
T7881 20218-20226 VB denotes detected
T7882 20227-20229 IN denotes in
T7883 20230-20237 NN denotes samples
T7884 20238-20246 VB denotes prepared
T7885 20247-20251 IN denotes from
T7886 20252-20260 JJ denotes parental
T7887 20261-20266 NN denotes cells
T7888 20266-20267 -COMMA- denotes ,
T7889 20268-20281 VB denotes demonstrating
T7890 20282-20286 IN denotes that
T7891 20287-20290 DT denotes the
T7892 20291-20298 NN denotes defects
T7893 20299-20301 IN denotes in
T7894 20302-20305 NN denotes IKK
T7895 20306-20317 NN denotes recruitment
T7896 20318-20320 PRP denotes we
T7897 20321-20329 VB denotes observed
T7898 20330-20334 VB denotes were
T7899 20335-20338 JJ denotes due
T7900 20339-20351 RB denotes specifically
T7901 20352-20354 TO denotes to
T7902 20355-20358 DT denotes the
T7903 20359-20366 NN denotes absence
T7904 20367-20369 IN denotes of
T7905 20370-20374 NN denotes HOIP
T7906 20375-20385 NN denotes expression
T7907 20387-20392 DT denotes These
T7908 20393-20397 NN denotes data
T7909 20398-20406 VB denotes indicate
T7910 20407-20411 IN denotes that
T7911 20412-20416 NN denotes HOIP
T7912 20417-20419 VB denotes is
T7913 20420-20428 VB denotes required
T7914 20429-20432 IN denotes for
T7915 20433-20444 NN denotes recruitment
T7916 20445-20447 IN denotes of
T7917 20448-20451 DT denotes the
T7918 20452-20468 JJ denotes NF-κB-activating
T7919 20469-20476 NN denotes complex
T7920 20477-20479 TO denotes to
T7921 20480-20483 DT denotes the
T7922 20484-20488 NN denotes CD40
T7923 20489-20498 NN denotes signaling
T7924 20499-20506 NN denotes complex
T7925 20508-20510 IN denotes As
T7926 20511-20516 VB denotes shown
T7927 20517-20521 RB denotes here
T7928 20522-20525 CC denotes and
T7929 20526-20528 IN denotes in
T7930 20529-20532 PRP-DOLLAR- denotes our
T7931 20533-20541 JJ denotes previous
T7932 20542-20547 NN denotes study
T7933 20548-20549 -LRB- denotes [
T7934 20549-20550 CD denotes 6
T7935 20550-20551 -RRB- denotes ]
T7936 20551-20552 -COMMA- denotes ,
T7937 20553-20557 NN denotes IKKγ
T7938 20558-20565 JJ denotes present
T7939 20566-20568 IN denotes in
T7940 20569-20573 NN denotes CD40
T7941 20574-20592 NN denotes immunoprecipitates
T7942 20593-20600 VB denotes appears
T7943 20601-20603 TO denotes to
T7944 20604-20608 VB denotes have
T7945 20609-20610 DT denotes a
T7946 20611-20617 JJ denotes higher
T7947 20618-20627 JJ denotes molecular
T7948 20628-20634 NN denotes weight
T7949 20635-20639 IN denotes than
T7950 20640-20644 DT denotes that
T7951 20645-20650 VB denotes found
T7952 20651-20653 IN denotes in
T7953 20654-20658 NN denotes cell
T7954 20659-20666 NN denotes lysates
T7955 20668-20670 TO denotes To
T7956 20671-20678 VB denotes confirm
T7957 20679-20683 IN denotes that
T7958 20684-20688 DT denotes this
T7959 20689-20695 JJ denotes higher
T7960 20696-20705 JJ denotes molecular
T7961 20706-20712 NN denotes weight
T7962 20713-20720 NN denotes species
T7963 20721-20724 VB denotes was
T7964 20725-20731 RB denotes indeed
T7965 20732-20736 NN denotes IKKγ
T7966 20736-20737 -COMMA- denotes ,
T7967 20738-20740 PRP denotes we
T7968 20741-20750 VB denotes generated
T7969 20751-20757 NN denotes A20.2J
T7970 20758-20762 NN denotes cell
T7971 20763-20768 NN denotes lines
T7972 20769-20779 VB denotes containing
T7973 20780-20781 DT denotes a
T7974 20782-20788 RB denotes stably
T7975 20789-20799 VB denotes integrated
T7976 20800-20810 JJ denotes retroviral
T7977 20811-20817 NN denotes vector
T7978 20818-20822 WDT denotes that
T7979 20823-20830 VB denotes encoded
T7980 20831-20833 DT denotes an
T7981 20834-20848 JJ denotes epitope-tagged
T7982 20849-20856 NN denotes version
T7983 20857-20859 IN denotes of
T7984 20860-20865 NN denotes mouse
T7985 20866-20870 NN denotes IKKγ
T7986 20872-20876 NN denotes Cell
T7987 20877-20884 NN denotes lysates
T7988 20885-20888 CC denotes and
T7989 20889-20893 NN denotes CD40
T7990 20894-20912 NN denotes immunoprecipitates
T7991 20913-20921 VB denotes prepared
T7992 20922-20926 IN denotes from
T7993 20927-20932 DT denotes these
T7994 20933-20938 NN denotes cells
T7995 20939-20943 VB denotes were
T7996 20944-20952 VB denotes analyzed
T7997 20953-20955 IN denotes by
T7998 20956-20963 JJ denotes Western
T7999 20964-20972 NN denotes blotting
T8000 20973-20976 IN denotes for
T8001 20977-20980 DT denotes the
T8002 20981-20988 NN denotes epitope
T8003 20989-20992 NN denotes tag
T8004 20993-20994 -LRB- denotes (
T8005 20994-20998 NN denotes Fig.
T8006 20999-21001 NN denotes 5C
T8007 21001-21002 -RRB- denotes )
T8008 21004-21008 DT denotes This
T8009 21009-21017 NN denotes analysis
T8010 21018-21026 VB denotes produced
T8011 21027-21028 DT denotes a
T8012 21029-21036 NN denotes pattern
T8013 21037-21039 IN denotes of
T8014 21040-21045 NN denotes bands
T8015 21046-21050 WDT denotes that
T8016 21051-21054 VB denotes was
T8017 21055-21066 RB denotes essentially
T8018 21067-21070 DT denotes the
T8019 21071-21075 JJ denotes same
T8020 21076-21078 IN denotes as
T8021 21079-21083 DT denotes that
T8022 21084-21092 VB denotes obtained
T8023 21093-21098 VB denotes using
T8024 21099-21101 DT denotes an
T8025 21102-21110 NN denotes antibody
T8026 21111-21119 JJ denotes specific
T8027 21120-21123 IN denotes for
T8028 21124-21130 JJ denotes native
T8029 21131-21135 NN denotes IKKγ
T8030 21136-21137 -LRB- denotes (
T8031 21137-21144 NN denotes compare
T8032 21145-21151 NN denotes panels
T8033 21152-21153 NN denotes B
T8034 21154-21157 CC denotes and
T8035 21158-21159 NN denotes C
T8036 21160-21162 IN denotes in
T8037 21163-21167 NNP denotes Fig.
T8038 21168-21169 CD denotes 5
T8039 21169-21170 -RRB- denotes )
T8040 21172-21180 RB denotes Moreover
T8041 21180-21181 -COMMA- denotes ,
T8042 21182-21190 NN denotes analysis
T8043 21191-21193 IN denotes of
T8044 21194-21199 NN denotes cells
T8045 21200-21210 VB denotes expressing
T8046 21211-21218 NN denotes epitope
T8047 21219-21230 NN denotes tagged-IKKγ
T8048 21231-21240 VB denotes confirmed
T8049 21241-21245 IN denotes that
T8050 21246-21257 NN denotes recruitment
T8051 21258-21260 IN denotes of
T8052 21261-21265 NN denotes IKKγ
T8053 21266-21268 TO denotes to
T8054 21269-21273 NN denotes CD40
T8055 21274-21282 VB denotes requires
T8056 21283-21287 NN denotes HOIP
T8057 21289-21291 TO denotes To
T8058 21292-21301 VB denotes determine
T8059 21302-21306 WP denotes what
T8060 21307-21310 VB denotes was
T8061 21311-21322 JJ denotes responsible
T8062 21323-21326 IN denotes for
T8063 21327-21330 DT denotes the
T8064 21331-21340 VB denotes increased
T8065 21341-21350 JJ denotes molecular
T8066 21351-21357 NN denotes weight
T8067 21358-21360 IN denotes of
T8068 21361-21365 NN denotes IKKγ
T8069 21366-21368 IN denotes in
T8070 21369-21373 NN denotes CD40
T8071 21374-21392 NN denotes immunoprecipitates
T8072 21392-21393 -COMMA- denotes ,
T8073 21394-21397 DT denotes the
T8074 21398-21405 NN denotes protein
T8075 21406-21413 NN denotes samples
T8076 21414-21418 VB denotes were
T8077 21419-21428 VB denotes incubated
T8078 21429-21433 IN denotes with
T8079 21434-21440 NN denotes lambda
T8080 21441-21452 NN denotes phosphatase
T8081 21452-21453 -COMMA- denotes ,
T8082 21454-21459 WDT denotes which
T8083 21460-21467 VB denotes removes
T8084 21468-21478 NN denotes phosphates
T8085 21479-21487 VB denotes attached
T8086 21488-21490 TO denotes to
T8087 21491-21499 NN denotes tyrosine
T8088 21499-21500 -COMMA- denotes ,
T8089 21501-21510 NN denotes threonine
T8090 21510-21511 -COMMA- denotes ,
T8091 21512-21514 CC denotes or
T8092 21515-21521 NN denotes serine
T8093 21522-21530 NN denotes residues
T8094 21531-21533 IN denotes in
T8095 21534-21542 NN denotes proteins
T8096 21544-21548 DT denotes This
T8097 21549-21558 NN denotes treatment
T8098 21559-21566 VB denotes reduced
T8099 21567-21571 JJ denotes much
T8100 21572-21574 IN denotes of
T8101 21575-21590 JJ denotes CD40-associated
T8102 21591-21595 NN denotes IKKγ
T8103 21596-21598 TO denotes to
T8104 21599-21601 DT denotes an
T8105 21602-21610 JJ denotes apparent
T8106 21611-21620 JJ denotes molecular
T8107 21621-21627 NN denotes weight
T8108 21628-21635 JJ denotes similar
T8109 21636-21638 TO denotes to
T8110 21639-21643 DT denotes that
T8111 21644-21646 IN denotes of
T8112 21647-21650 DT denotes the
T8113 21651-21658 NN denotes protein
T8114 21659-21661 IN denotes in
T8115 21662-21666 NN denotes cell
T8116 21667-21674 NN denotes lysates
T8117 21675-21676 -LRB- denotes (
T8118 21676-21680 NNP denotes Fig.
T8119 21681-21683 NN denotes 5C
T8120 21683-21684 -RRB- denotes )
T8121 21686-21693 RB denotes However
T8122 21693-21694 -COMMA- denotes ,
T8123 21695-21697 IN denotes at
T8124 21698-21703 JJ denotes least
T8125 21704-21707 CD denotes one
T8126 21708-21712 NN denotes band
T8127 21713-21715 IN denotes of
T8128 21716-21729 RB denotes significantly
T8129 21730-21736 JJ denotes higher
T8130 21737-21746 JJ denotes molecular
T8131 21747-21753 NN denotes weight
T8132 21754-21762 VB denotes remained
T8133 21763-21768 IN denotes after
T8134 21769-21780 NN denotes phosphatase
T8135 21781-21790 NN denotes treatment
T8136 21790-21791 -COMMA- denotes ,
T8137 21792-21802 VB denotes suggesting
T8138 21803-21807 IN denotes that
T8139 21808-21823 JJ denotes CD40-associated
T8140 21824-21828 NN denotes IKKγ
T8141 21829-21831 VB denotes is
T8142 21832-21839 JJ denotes subject
T8143 21840-21842 TO denotes to
T8144 21843-21845 IN denotes at
T8145 21846-21851 JJ denotes least
T8146 21852-21855 CD denotes one
T8147 21856-21861 JJ denotes other
T8148 21862-21874 NN denotes modification
T8149 21875-21877 IN denotes in
T8150 21878-21886 NN denotes addition
T8151 21887-21889 TO denotes to
T8152 21890-21905 NN denotes phosphorylation
T8153 21907-21914 RB denotes Overall
T8154 21914-21915 -COMMA- denotes ,
T8155 21916-21921 DT denotes these
T8156 21922-21926 NN denotes data
T8157 21927-21938 VB denotes demonstrate
T8158 21939-21943 IN denotes that
T8159 21944-21948 NN denotes HOIP
T8160 21949-21951 VB denotes is
T8161 21952-21960 VB denotes required
T8162 21961-21964 IN denotes for
T8163 21965-21968 DT denotes the
T8164 21969-21980 NN denotes association
T8165 21981-21983 IN denotes of
T8166 21984-21988 NN denotes IKKγ
T8167 21989-21993 IN denotes with
T8168 21994-21998 NN denotes CD40
T8169 21998-21999 -COMMA- denotes ,
T8170 22000-22003 CC denotes and
T8171 22004-22011 VB denotes suggest
T8172 22012-22016 IN denotes that
T8173 22017-22021 DT denotes this
T8174 22022-22027 NN denotes event
T8175 22028-22030 VB denotes is
T8176 22031-22038 VB denotes coupled
T8177 22039-22041 TO denotes to
T8178 22042-22060 JJ denotes post-translational
T8179 22061-22074 NN denotes modifications
T8180 22075-22077 IN denotes of
T8181 22078-22082 NN denotes IKKγ
T10198 22096-22099 PRP-DOLLAR- denotes Our
T10199 22100-22107 NN denotes results
T10200 22108-22116 VB denotes indicate
T10201 22117-22121 IN denotes that
T10202 22122-22125 DT denotes the
T10203 22126-22133 NN denotes protein
T10204 22134-22138 NN denotes HOIP
T10205 22139-22141 VB denotes is
T10206 22142-22150 JJ denotes critical
T10207 22151-22154 IN denotes for
T10208 22155-22167 JJ denotes CD40-induced
T10209 22168-22175 NN denotes signals
T10210 22176-22180 WDT denotes that
T10211 22181-22189 VB denotes regulate
T10212 22190-22191 NN denotes B
T10213 22192-22196 NN denotes cell
T10214 22197-22205 NN denotes function
T10215 22207-22210 PRP-DOLLAR- denotes Our
T10216 22211-22215 NN denotes data
T10217 22216-22220 VB denotes show
T10218 22221-22225 IN denotes that
T10219 22226-22240 JJ denotes HOIP-dependent
T10220 22241-22249 JJ denotes cellular
T10221 22250-22259 NN denotes responses
T10222 22260-22267 VB denotes include
T10223 22268-22281 JJ denotes CD40-mediated
T10224 22282-22294 NN denotes upregulation
T10225 22295-22297 IN denotes of
T10226 22298-22302 NN denotes CD80
T10227 22303-22313 NN denotes expression
T10228 22314-22317 CC denotes and
T10229 22318-22327 NN denotes synthesis
T10230 22328-22330 IN denotes of
T10231 22331-22339 NN denotes germline
T10232 22340-22343 NN denotes RNA
T10233 22344-22355 NN denotes transcripts
T10234 22356-22360 IN denotes from
T10235 22361-22364 DT denotes the
T10236 22365-22379 NN denotes immunoglobulin
T10237 22380-22385 JJ denotes heavy
T10238 22386-22391 NN denotes chain
T10239 22392-22397 NN denotes locus
T10240 22397-22398 -COMMA- denotes ,
T10241 22399-22402 CD denotes two
T10242 22403-22409 NN denotes events
T10243 22410-22414 WDT denotes that
T10244 22415-22418 VB denotes are
T10245 22419-22428 JJ denotes important
T10246 22429-22432 IN denotes for
T10247 22433-22449 JJ denotes T-cell-dependent
T10248 22450-22467 JJ denotes antibody-mediated
T10249 22468-22474 JJ denotes immune
T10250 22475-22484 NN denotes responses
T10251 22486-22488 IN denotes At
T10252 22489-22492 DT denotes the
T10253 22493-22502 JJ denotes molecular
T10254 22503-22508 NN denotes level
T10255 22508-22509 -COMMA- denotes ,
T10256 22510-22513 PRP-DOLLAR- denotes our
T10257 22514-22518 NN denotes data
T10258 22519-22527 VB denotes indicate
T10259 22528-22532 IN denotes that
T10260 22533-22537 NN denotes HOIP
T10261 22538-22547 NN denotes functions
T10262 22548-22558 JJ denotes downstream
T10263 22559-22561 IN denotes of
T10264 22562-22567 NN denotes TRAF2
T10265 22568-22570 IN denotes in
T10266 22571-22574 DT denotes the
T10267 22575-22579 NN denotes CD40
T10268 22580-22589 NN denotes signaling
T10269 22590-22597 NN denotes pathway
T10270 22598-22601 CC denotes and
T10271 22602-22606 IN denotes that
T10272 22607-22611 NN denotes HOIP
T10273 22612-22615 VB denotes has
T10274 22616-22617 DT denotes a
T10275 22618-22621 JJ denotes key
T10276 22622-22626 NN denotes role
T10277 22627-22629 IN denotes in
T10278 22630-22639 VB denotes promoting
T10279 22640-22643 DT denotes the
T10280 22644-22655 NN denotes recruitment
T10281 22656-22658 IN denotes of
T10282 22659-22662 DT denotes the
T10283 22663-22666 NN denotes IKK
T10284 22667-22674 NN denotes complex
T10285 22675-22677 TO denotes to
T10286 22678-22682 NN denotes CD40
T10287 22684-22694 JJ denotes Consistent
T10288 22695-22699 IN denotes with
T10289 22700-22704 DT denotes this
T10290 22704-22705 -COMMA- denotes ,
T10291 22706-22718 JJ denotes CD40-induced
T10292 22719-22729 NN denotes activation
T10293 22730-22732 IN denotes of
T10294 22733-22738 NN denotes NF-κB
T10295 22739-22741 VB denotes is
T10296 22742-22751 JJ denotes dependent
T10297 22752-22754 IN denotes on
T10298 22755-22758 DT denotes the
T10299 22759-22767 NN denotes presence
T10300 22768-22770 IN denotes of
T10301 22771-22775 NN denotes HOIP
T10302 22777-22779 IN denotes In
T10303 22780-22788 NN denotes addition
T10304 22788-22789 -COMMA- denotes ,
T10305 22790-22793 PRP-DOLLAR- denotes our
T10306 22794-22798 NN denotes data
T10307 22799-22803 VB denotes show
T10308 22804-22808 IN denotes that
T10309 22809-22813 NN denotes HOIP
T10310 22814-22825 VB denotes facilitates
T10311 22826-22829 DT denotes the
T10312 22830-22840 NN denotes activation
T10313 22841-22843 IN denotes of
T10314 22844-22847 NN denotes JNK
T10315 22848-22850 IN denotes in
T10316 22851-22859 NN denotes response
T10317 22860-22862 TO denotes to
T10318 22863-22867 NN denotes CD40
T10319 22868-22878 NN denotes engagement
T10320 22880-22888 RB denotes Together
T10321 22888-22889 -COMMA- denotes ,
T10322 22890-22893 PRP-DOLLAR- denotes our
T10323 22894-22902 NN denotes findings
T10324 22903-22910 VB denotes provide
T10325 22911-22918 NN denotes support
T10326 22919-22922 IN denotes for
T10327 22923-22926 DT denotes the
T10328 22927-22937 NN denotes conclusion
T10329 22938-22942 IN denotes that
T10330 22943-22947 NN denotes HOIP
T10331 22948-22950 VB denotes is
T10332 22951-22952 DT denotes a
T10333 22953-22956 JJ denotes key
T10334 22957-22966 NN denotes component
T10335 22967-22969 IN denotes of
T10336 22970-22973 DT denotes the
T10337 22974-22978 NN denotes CD40
T10338 22979-22988 NN denotes signaling
T10339 22989-22996 NN denotes pathway
T10340 22998-23003 VB denotes Given
T10341 23004-23007 DT denotes the
T10342 23008-23018 NN denotes importance
T10343 23019-23021 IN denotes of
T10344 23022-23026 NN denotes CD40
T10345 23027-23036 NN denotes signaling
T10346 23037-23039 IN denotes in
T10347 23040-23044 CC denotes both
T10348 23045-23053 JJ denotes cellular
T10349 23054-23057 CC denotes and
T10350 23058-23065 JJ denotes humoral
T10351 23066-23072 JJ denotes immune
T10352 23073-23082 NN denotes responses
T10353 23082-23083 -COMMA- denotes ,
T10354 23084-23087 PRP-DOLLAR- denotes our
T10355 23088-23095 NN denotes results
T10356 23096-23104 VB denotes indicate
T10357 23105-23109 IN denotes that
T10358 23110-23114 NN denotes HOIP
T10359 23115-23118 VB denotes has
T10360 23119-23120 DT denotes a
T10361 23121-23129 JJ denotes critical
T10362 23130-23134 NN denotes role
T10363 23135-23137 IN denotes in
T10364 23138-23141 DT denotes the
T10365 23142-23152 NN denotes regulation
T10366 23153-23155 IN denotes of
T10367 23156-23159 DT denotes the
T10368 23160-23166 JJ denotes immune
T10369 23167-23173 NN denotes system
T10370 23175-23178 DT denotes The
T10371 23179-23189 JJ denotes functional
T10372 23190-23200 NN denotes properties
T10373 23201-23203 IN denotes of
T10374 23204-23208 NN denotes HOIP
T10375 23209-23213 VB denotes have
T10376 23214-23218 RB denotes only
T10377 23219-23223 VB denotes been
T10378 23224-23233 RB denotes partially
T10379 23234-23247 VB denotes characterized
T10380 23249-23256 JJ denotes Initial
T10381 23257-23264 NN denotes studies
T10382 23265-23271 VB denotes showed
T10383 23272-23276 IN denotes that
T10384 23277-23281 NN denotes HOIP
T10385 23282-23285 CC denotes and
T10386 23286-23289 DT denotes the
T10387 23290-23297 VB denotes related
T10388 23298-23305 NN denotes protein
T10389 23306-23312 NN denotes HOIL-1
T10390 23313-23316 VB denotes are
T10391 23317-23327 NN denotes components
T10392 23328-23330 IN denotes of
T10393 23331-23332 DT denotes a
T10394 23333-23338 JJ denotes large
T10395 23339-23340 -LRB- denotes (
T10396 23340-23344 CD denotes ∼600
T10397 23345-23348 NN denotes kDa
T10398 23348-23349 -RRB- denotes )
T10399 23350-23357 NN denotes protein
T10400 23358-23365 NN denotes complex
T10401 23366-23373 JJ denotes capable
T10402 23374-23376 IN denotes of
T10403 23377-23389 VB denotes synthesizing
T10404 23390-23396 JJ denotes linear
T10405 23397-23410 NN denotes polyubiquitin
T10406 23411-23417 NN denotes chains
T10407 23418-23419 -LRB- denotes [
T10408 23419-23420 CD denotes 5
T10409 23420-23421 -RRB- denotes ]
T10410 23423-23433 JJ denotes Subsequent
T10411 23434-23441 NN denotes studies
T10412 23442-23448 VB denotes showed
T10413 23449-23453 IN denotes that
T10414 23454-23455 DT denotes a
T10415 23456-23471 JJ denotes HOIP-containing
T10416 23472-23479 NN denotes complex
T10417 23480-23483 MD denotes can
T10418 23484-23492 VB denotes interact
T10419 23493-23497 IN denotes with
T10420 23498-23502 NN denotes IKKγ
T10421 23503-23506 CC denotes and
T10422 23507-23517 VB denotes facilitate
T10423 23518-23528 NN denotes activation
T10424 23529-23531 IN denotes of
T10425 23532-23537 NN denotes NF-κB
T10426 23538-23541 IN denotes via
T10427 23542-23545 DT denotes the
T10428 23546-23555 JJ denotes canonical
T10429 23556-23563 NN denotes pathway
T10430 23564-23565 -LRB- denotes [
T10431 23565-23567 CD denotes 16
T10432 23567-23568 -RRB- denotes ]
T10433 23570-23575 DT denotes These
T10434 23576-23580 NN denotes data
T10435 23580-23581 -COMMA- denotes ,
T10436 23582-23592 VB denotes considered
T10437 23593-23601 RB denotes together
T10438 23602-23606 IN denotes with
T10439 23607-23611 NN denotes ours
T10440 23611-23612 -COMMA- denotes ,
T10441 23613-23620 VB denotes suggest
T10442 23621-23625 IN denotes that
T10443 23626-23627 DT denotes a
T10444 23628-23643 JJ denotes HOIP-containing
T10445 23644-23651 NN denotes complex
T10446 23652-23660 VB denotes mediates
T10447 23661-23672 NN denotes recruitment
T10448 23673-23675 IN denotes of
T10449 23676-23680 NN denotes IKKγ
T10450 23681-23683 TO denotes to
T10451 23684-23687 DT denotes the
T10452 23688-23692 NN denotes CD40
T10453 23693-23702 NN denotes signaling
T10454 23703-23710 NN denotes complex
T10455 23712-23714 IN denotes In
T10456 23715-23723 NN denotes addition
T10457 23723-23724 -COMMA- denotes ,
T10458 23725-23740 JJ denotes CD40-associated
T10459 23741-23745 NN denotes HOIP
T10460 23746-23751 MD denotes could
T10461 23752-23756 VB denotes play
T10462 23757-23758 DT denotes a
T10463 23759-23763 NN denotes role
T10464 23764-23766 IN denotes in
T10465 23767-23777 VB denotes activating
T10466 23778-23782 NN denotes IKKγ
T10467 23783-23788 IN denotes after
T10468 23789-23792 PRP-DOLLAR- denotes its
T10469 23793-23804 NN denotes recruitment
T10470 23805-23807 TO denotes to
T10471 23808-23811 DT denotes the
T10472 23812-23821 NN denotes signaling
T10473 23822-23829 NN denotes complex
T10474 23830-23831 -LRB- denotes [
T10475 23831-23833 CD denotes 16
T10476 23833-23834 -RRB- denotes ]
T10477 23836-23839 DT denotes The
T10478 23840-23846 JJ denotes higher
T10479 23847-23856 JJ denotes molecular
T10480 23857-23863 NN denotes weight
T10481 23864-23869 NN denotes forms
T10482 23870-23872 IN denotes of
T10483 23873-23877 NN denotes IKKγ
T10484 23878-23880 PRP denotes we
T10485 23881-23889 VB denotes observed
T10486 23890-23892 IN denotes in
T10487 23893-23897 NN denotes CD40
T10488 23898-23916 NN denotes immunoprecipitates
T10489 23917-23922 MD denotes would
T10490 23923-23925 VB denotes be
T10491 23926-23936 JJ denotes consistent
T10492 23937-23941 IN denotes with
T10493 23942-23945 DT denotes the
T10494 23946-23954 NN denotes presence
T10495 23955-23957 IN denotes of
T10496 23958-23978 JJ denotes post-transcriptional
T10497 23979-23992 NN denotes modifications
T10498 23993-24002 VB denotes including
T10499 24003-24018 NN denotes phosphorylation
T10500 24019-24022 CC denotes and
T10501 24023-24037 NN denotes ubiquitination
T10502 24037-24038 -COMMA- denotes ,
T10503 24039-24044 WDT denotes which
T10504 24045-24049 VB denotes have
T10505 24050-24054 VB denotes been
T10506 24055-24064 VB denotes suggested
T10507 24065-24067 TO denotes to
T10508 24068-24074 VB denotes reduce
T10509 24075-24077 CC denotes or
T10510 24078-24085 VB denotes enhance
T10511 24086-24090 NN denotes IKKγ
T10512 24091-24099 NN denotes activity
T10513 24099-24100 -COMMA- denotes ,
T10514 24101-24113 RB denotes respectively
T10515 24114-24115 -LRB- denotes [
T10516 24115-24117 CD denotes 16
T10517 24117-24118 -RRB- denotes ]
T10518 24118-24119 -COMMA- denotes ,
T10519 24120-24121 -LRB- denotes [
T10520 24121-24123 CD denotes 17
T10521 24123-24124 -RRB- denotes ]
T10522 24126-24129 DT denotes The
T10523 24130-24140 NN denotes mechanisms
T10524 24141-24143 IN denotes by
T10525 24144-24149 WDT denotes which
T10526 24150-24154 NN denotes HOIP
T10527 24155-24163 VB denotes mediates
T10528 24164-24175 NN denotes recruitment
T10529 24176-24178 IN denotes of
T10530 24179-24182 DT denotes the
T10531 24183-24186 NN denotes IKK
T10532 24187-24194 NN denotes complex
T10533 24195-24197 TO denotes to
T10534 24198-24202 NN denotes CD40
T10535 24203-24206 CC denotes and
T10536 24207-24209 IN denotes by
T10537 24210-24215 WDT denotes which
T10538 24216-24220 NN denotes HOIP
T10539 24221-24223 VB denotes is
T10540 24224-24233 VB denotes recruited
T10541 24234-24236 TO denotes to
T10542 24237-24241 NN denotes CD40
T10543 24242-24248 VB denotes remain
T10544 24249-24256 JJ denotes unclear
T10545 24258-24259 DT denotes A
T10546 24260-24268 JJ denotes previous
T10547 24269-24274 NN denotes study
T10548 24275-24284 VB denotes indicates
T10549 24285-24289 IN denotes that
T10550 24290-24294 NN denotes HOIP
T10551 24295-24298 MD denotes may
T10552 24299-24306 VB denotes mediate
T10553 24307-24313 JJ denotes direct
T10554 24314-24322 NN denotes contacts
T10555 24323-24327 IN denotes with
T10556 24328-24331 DT denotes the
T10557 24332-24335 NN denotes IKK
T10558 24336-24343 NN denotes complex
T10559 24344-24345 -LRB- denotes [
T10560 24345-24347 CD denotes 16
T10561 24347-24348 -RRB- denotes ]
T10562 24348-24349 -COMMA- denotes ,
T10563 24350-24360 VB denotes suggesting
T10564 24361-24365 IN denotes that
T10565 24366-24368 PRP denotes it
T10566 24369-24378 VB denotes functions
T10567 24379-24381 IN denotes as
T10568 24382-24384 DT denotes an
T10569 24385-24392 NN denotes adaptor
T10570 24393-24396 IN denotes for
T10571 24397-24400 DT denotes the
T10572 24401-24412 NN denotes recruitment
T10573 24413-24415 IN denotes of
T10574 24416-24419 DT denotes the
T10575 24420-24423 NN denotes IKK
T10576 24424-24431 NN denotes complex
T10577 24432-24434 TO denotes to
T10578 24435-24439 NN denotes CD40
T10579 24441-24448 RB denotes However
T10580 24448-24449 -COMMA- denotes ,
T10581 24450-24453 DT denotes the
T10582 24454-24463 NN denotes ubiquitin
T10583 24464-24470 NN denotes ligase
T10584 24471-24479 NN denotes activity
T10585 24480-24482 IN denotes of
T10586 24483-24487 NN denotes HOIP
T10587 24488-24496 VB denotes suggests
T10588 24497-24501 IN denotes that
T10589 24502-24504 PRP denotes it
T10590 24505-24507 VB denotes is
T10591 24508-24512 JJ denotes more
T10592 24513-24517 IN denotes than
T10593 24518-24519 DT denotes a
T10594 24520-24526 JJ denotes simple
T10595 24527-24534 NN denotes adapter
T10596 24535-24543 NN denotes molecule
T10597 24545-24547 IN denotes As
T10598 24548-24552 NN denotes IKKγ
T10599 24553-24560 VB denotes appears
T10600 24561-24568 JJ denotes capable
T10601 24569-24571 IN denotes of
T10602 24572-24579 VB denotes binding
T10603 24580-24586 JJ denotes linear
T10604 24587-24600 NN denotes polyubiquitin
T10605 24601-24602 -LRB- denotes [
T10606 24602-24604 CD denotes 18
T10607 24604-24605 -RRB- denotes ]
T10608 24605-24606 -COMMA- denotes ,
T10609 24607-24608 -LRB- denotes [
T10610 24608-24610 CD denotes 19
T10611 24610-24611 -RRB- denotes ]
T10612 24611-24612 -COMMA- denotes ,
T10613 24613-24615 PRP denotes it
T10614 24616-24618 VB denotes is
T10615 24619-24627 JJ denotes possible
T10616 24628-24632 IN denotes that
T10617 24633-24637 NN denotes HOIP
T10618 24638-24645 VB denotes directs
T10619 24646-24655 NN denotes formation
T10620 24656-24658 IN denotes of
T10621 24659-24665 JJ denotes linear
T10622 24666-24679 NN denotes polyubiquitin
T10623 24680-24686 NN denotes chains
T10624 24687-24689 IN denotes on
T10625 24690-24691 DT denotes a
T10626 24692-24707 JJ denotes CD40-associated
T10627 24708-24714 NN denotes factor
T10628 24714-24715 -COMMA- denotes ,
T10629 24716-24719 CC denotes and
T10630 24720-24722 PRP denotes it
T10631 24723-24725 VB denotes is
T10632 24726-24731 DT denotes these
T10633 24732-24738 NN denotes chains
T10634 24739-24743 WDT denotes that
T10635 24744-24749 VB denotes serve
T10636 24750-24752 TO denotes to
T10637 24753-24760 VB denotes recruit
T10638 24761-24765 NN denotes IKKγ
T10639 24766-24768 TO denotes to
T10640 24769-24772 DT denotes the
T10641 24773-24777 NN denotes CD40
T10642 24778-24787 NN denotes signaling
T10643 24788-24795 NN denotes complex
T10644 24797-24800 DT denotes The
T10645 24801-24810 JJ denotes molecular
T10646 24811-24823 NN denotes interactions
T10647 24824-24833 JJ denotes necessary
T10648 24834-24837 IN denotes for
T10649 24838-24849 NN denotes recruitment
T10650 24850-24852 IN denotes of
T10651 24853-24857 NN denotes HOIP
T10652 24858-24864 PRP denotes itself
T10653 24865-24867 TO denotes to
T10654 24868-24871 DT denotes the
T10655 24872-24876 NN denotes CD40
T10656 24877-24886 NN denotes signaling
T10657 24887-24894 NN denotes complex
T10658 24895-24899 RB denotes also
T10659 24900-24906 VB denotes remain
T10660 24907-24909 TO denotes to
T10661 24910-24912 VB denotes be
T10662 24913-24918 RB denotes fully
T10663 24919-24932 VB denotes characterized
T10664 24934-24936 PRP denotes We
T10665 24937-24947 RB denotes previously
T10666 24948-24954 VB denotes showed
T10667 24955-24959 IN denotes that
T10668 24960-24963 DT denotes the
T10669 24964-24975 NN denotes recruitment
T10670 24976-24978 IN denotes of
T10671 24979-24983 NN denotes HOIP
T10672 24984-24986 TO denotes to
T10673 24987-24990 DT denotes the
T10674 24991-25000 NN denotes signaling
T10675 25001-25008 NN denotes complex
T10676 25009-25011 VB denotes is
T10677 25012-25027 JJ denotes TRAF2-dependent
T10678 25028-25029 -LRB- denotes [
T10679 25029-25030 CD denotes 8
T10680 25030-25031 -RRB- denotes ]
T10681 25033-25044 RB denotes Potentially
T10682 25044-25045 -COMMA- denotes ,
T10683 25046-25051 NN denotes TRAF2
T10684 25052-25055 CC denotes and
T10685 25056-25060 NN denotes HOIP
T10686 25061-25069 RB denotes directly
T10687 25070-25078 VB denotes interact
T10688 25078-25079 -COMMA- denotes ,
T10689 25080-25083 CC denotes but
T10690 25084-25086 PRP denotes it
T10691 25087-25089 VB denotes is
T10692 25090-25098 JJ denotes possible
T10693 25099-25103 IN denotes that
T10694 25104-25107 DT denotes the
T10695 25108-25117 NN denotes ubiquitin
T10696 25118-25124 NN denotes ligase
T10697 25125-25133 NN denotes activity
T10698 25134-25136 IN denotes of
T10699 25137-25142 NN denotes TRAF2
T10700 25143-25144 -LRB- denotes [
T10701 25144-25146 CD denotes 20
T10702 25146-25147 -RRB- denotes ]
T10703 25148-25149 -LRB- denotes (
T10704 25149-25151 CC denotes or
T10705 25152-25168 JJ denotes TRAF2-associated
T10706 25169-25177 NN denotes proteins
T10707 25177-25178 -COMMA- denotes ,
T10708 25179-25183 JJ denotes such
T10709 25184-25186 IN denotes as
T10710 25187-25190 DT denotes the
T10711 25191-25196 NN denotes cIAPs
T10712 25197-25198 -LRB- denotes [
T10713 25198-25200 CD denotes 21
T10714 25200-25201 -RRB- denotes ]
T10715 25201-25202 -RRB- denotes )
T10716 25203-25212 VB denotes generates
T10717 25213-25223 JJ denotes K63-linked
T10718 25224-25237 NN denotes polyubiquitin
T10719 25238-25244 NN denotes chains
T10720 25245-25247 TO denotes to
T10721 25248-25253 WDT denotes which
T10722 25254-25258 NN denotes HOIP
T10723 25259-25262 MD denotes can
T10724 25263-25267 VB denotes bind
T10725 25268-25271 CC denotes and
T10726 25272-25276 RB denotes thus
T10727 25277-25286 VB denotes associate
T10728 25287-25291 IN denotes with
T10729 25292-25295 DT denotes the
T10730 25296-25300 NN denotes CD40
T10731 25301-25310 NN denotes signaling
T10732 25311-25318 NN denotes complex
T10733 25319-25320 -LRB- denotes [
T10734 25320-25322 CD denotes 22
T10735 25322-25323 -RRB- denotes ]
T10736 25325-25330 IN denotes While
T10737 25331-25334 PRP-DOLLAR- denotes our
T10738 25335-25343 JJ denotes previous
T10739 25344-25348 NN denotes work
T10740 25349-25358 VB denotes indicated
T10741 25359-25360 DT denotes a
T10742 25361-25372 RB denotes potentially
T10743 25373-25382 JJ denotes important
T10744 25383-25387 NN denotes link
T10745 25388-25395 IN denotes between
T10746 25396-25401 NN denotes TRAF2
T10747 25402-25405 CC denotes and
T10748 25406-25410 NN denotes HOIP
T10749 25411-25413 IN denotes in
T10750 25414-25418 NN denotes CD40
T10751 25419-25428 NN denotes signaling
T10752 25428-25429 -COMMA- denotes ,
T10753 25430-25433 DT denotes the
T10754 25434-25441 NN denotes signals
T10755 25442-25445 CC denotes and
T10756 25446-25455 NN denotes functions
T10757 25456-25462 VB denotes tested
T10758 25463-25467 RB denotes here
T10759 25468-25471 VB denotes are
T10760 25472-25481 JJ denotes dependent
T10761 25482-25486 IN denotes upon
T10762 25487-25492 NN denotes TRAF6
T10763 25493-25495 RB denotes as
T10764 25496-25500 RB denotes well
T10765 25501-25503 IN denotes as
T10766 25504-25509 NN denotes TRAF2
T10767 25511-25513 IN denotes In
T10768 25514-25522 JJ denotes previous
T10769 25523-25534 NN denotes experiments
T10770 25535-25539 IN denotes with
T10771 25540-25554 JJ denotes TRAF-deficient
T10772 25555-25561 NN denotes A20.2J
T10773 25562-25567 NN denotes cells
T10774 25567-25568 -COMMA- denotes ,
T10775 25569-25571 PRP denotes we
T10776 25572-25577 VB denotes found
T10777 25578-25582 IN denotes that
T10778 25583-25586 DT denotes the
T10779 25587-25597 NN denotes activation
T10780 25598-25600 IN denotes of
T10781 25601-25606 NN denotes NF-κB
T10782 25607-25609 IN denotes by
T10783 25610-25614 NN denotes CD40
T10784 25615-25620 MD denotes could
T10785 25621-25623 VB denotes be
T10786 25624-25632 VB denotes mediated
T10787 25633-25635 IN denotes by
T10788 25636-25642 CC denotes either
T10789 25643-25648 NN denotes TRAF2
T10790 25649-25651 CC denotes or
T10791 25652-25657 NN denotes TRAF6
T10792 25657-25658 -COMMA- denotes ,
T10793 25659-25664 IN denotes while
T10794 25665-25675 NN denotes activation
T10795 25676-25678 IN denotes of
T10796 25679-25682 NN denotes JNK
T10797 25683-25685 IN denotes by
T10798 25686-25690 NN denotes CD40
T10799 25691-25694 VB denotes was
T10800 25695-25702 RB denotes largely
T10801 25703-25712 JJ denotes dependent
T10802 25713-25715 IN denotes on
T10803 25716-25721 NN denotes TRAF6
T10804 25722-25727 RB denotes alone
T10805 25728-25729 -LRB- denotes [
T10806 25729-25730 CD denotes 8
T10807 25730-25731 -RRB- denotes ]
T10808 25733-25737 NN denotes HOIP
T10809 25738-25748 NN denotes deficiency
T10810 25749-25760 VB denotes compromises
T10811 25761-25764 DT denotes the
T10812 25765-25778 JJ denotes CD40-mediated
T10813 25779-25789 NN denotes activation
T10814 25790-25792 IN denotes of
T10815 25793-25797 CC denotes both
T10816 25798-25803 NN denotes NF-κB
T10817 25804-25807 CC denotes and
T10818 25808-25811 NN denotes JNK
T10819 25811-25812 -COMMA- denotes ,
T10820 25813-25823 VB denotes indicating
T10821 25824-25828 IN denotes that
T10822 25829-25836 NN denotes signals
T10823 25837-25845 VB denotes mediated
T10824 25846-25848 IN denotes by
T10825 25849-25853 CC denotes both
T10826 25854-25859 NN denotes TRAF2
T10827 25860-25863 CC denotes and
T10828 25864-25869 NN denotes TRAF6
T10829 25870-25876 RB denotes likely
T10830 25877-25881 VB denotes pass
T10831 25882-25889 IN denotes through
T10832 25890-25894 NN denotes HOIP
T10833 25896-25899 PRP-DOLLAR- denotes Our
T10834 25900-25908 JJ denotes previous
T10835 25909-25913 NN denotes work
T10836 25914-25918 RB denotes also
T10837 25919-25931 VB denotes demonstrated
T10838 25932-25936 IN denotes that
T10839 25937-25940 DT denotes the
T10840 25941-25954 JJ denotes CD40-mediated
T10841 25955-25965 NN denotes activation
T10842 25966-25968 IN denotes of
T10843 25969-25974 NN denotes NF-κB
T10844 25975-25978 CC denotes and
T10845 25979-25982 NN denotes JNK
T10846 25982-25983 -COMMA- denotes ,
T10847 25984-25989 IN denotes while
T10848 25990-26005 JJ denotes TRAF6-dependent
T10849 26005-26006 -COMMA- denotes ,
T10850 26007-26010 VB denotes was
T10851 26011-26014 RB denotes not
T10852 26015-26026 VB denotes compromised
T10853 26027-26029 IN denotes by
T10854 26030-26033 DT denotes the
T10855 26034-26044 NN denotes disruption
T10856 26045-26047 IN denotes of
T10857 26048-26051 DT denotes the
T10858 26052-26059 VB denotes binding
T10859 26060-26064 NN denotes site
T10860 26065-26068 IN denotes for
T10861 26069-26074 NN denotes TRAF6
T10862 26075-26077 IN denotes in
T10863 26078-26081 DT denotes the
T10864 26082-26093 JJ denotes cytoplasmic
T10865 26094-26100 NN denotes domain
T10866 26101-26103 IN denotes of
T10867 26104-26108 NN denotes CD40
T10868 26109-26111 CC denotes or
T10869 26112-26120 NN denotes deletion
T10870 26121-26123 IN denotes of
T10871 26124-26127 DT denotes the
T10872 26128-26136 NN denotes receptor
T10873 26137-26144 NN denotes binding
T10874 26145-26151 NN denotes domain
T10875 26152-26153 -LRB- denotes (
T10876 26153-26156 DT denotes the
T10877 26157-26163 NN denotes TRAF-C
T10878 26164-26170 NN denotes domain
T10879 26170-26171 -RRB- denotes )
T10880 26172-26174 IN denotes in
T10881 26175-26180 NN denotes TRAF6
T10882 26181-26182 -LRB- denotes [
T10883 26182-26183 CD denotes 8
T10884 26183-26184 -RRB- denotes ]
T10885 26186-26191 DT denotes These
T10886 26192-26204 NN denotes observations
T10887 26205-26213 VB denotes indicate
T10888 26214-26218 IN denotes that
T10889 26219-26224 NN denotes TRAF6
T10890 26225-26229 VB denotes need
T10891 26230-26233 RB denotes not
T10892 26234-26242 RB denotes directly
T10893 26243-26247 VB denotes bind
T10894 26248-26252 NN denotes CD40
T10895 26253-26255 IN denotes in
T10896 26256-26261 NN denotes order
T10897 26262-26264 TO denotes to
T10898 26265-26272 VB denotes mediate
T10899 26273-26280 JJ denotes certain
T10900 26281-26288 NN denotes signals
T10901 26288-26289 -COMMA- denotes ,
T10902 26290-26300 VB denotes suggesting
T10903 26301-26304 DT denotes the
T10904 26305-26313 NN denotes assembly
T10905 26314-26316 IN denotes of
T10906 26317-26318 DT denotes a
T10907 26319-26328 NN denotes signaling
T10908 26329-26336 NN denotes complex
T10909 26337-26340 RB denotes not
T10910 26341-26349 RB denotes directly
T10911 26350-26360 VB denotes associated
T10912 26361-26365 IN denotes with
T10913 26366-26369 DT denotes the
T10914 26370-26378 NN denotes receptor
T10915 26380-26382 IN denotes If
T10916 26383-26387 PDT denotes such
T10917 26388-26389 DT denotes a
T10918 26390-26397 NN denotes complex
T10919 26398-26404 VB denotes exists
T10920 26404-26405 -COMMA- denotes ,
T10921 26406-26409 PRP-DOLLAR- denotes our
T10922 26410-26417 NN denotes results
T10923 26418-26426 VB denotes indicate
T10924 26427-26431 IN denotes that
T10925 26432-26435 DT denotes the
T10926 26436-26443 NN denotes absence
T10927 26444-26446 IN denotes of
T10928 26447-26451 NN denotes HOIP
T10929 26452-26463 VB denotes compromises
T10930 26464-26467 PRP-DOLLAR- denotes its
T10931 26468-26476 NN denotes function
T10932 26477-26479 RB denotes as
T10933 26480-26484 RB denotes well
T10934 26486-26494 IN denotes Although
T10935 26495-26498 DT denotes the
T10936 26499-26510 NN denotes experiments
T10937 26511-26520 VB denotes presented
T10938 26521-26525 RB denotes here
T10939 26526-26531 VB denotes focus
T10940 26532-26534 IN denotes on
T10941 26535-26539 NN denotes CD40
T10942 26539-26540 -COMMA- denotes ,
T10943 26541-26544 PRP-DOLLAR- denotes our
T10944 26545-26552 NN denotes results
T10945 26553-26556 CC denotes and
T10946 26557-26562 DT denotes those
T10947 26563-26565 IN denotes of
T10948 26566-26571 JJ denotes other
T10949 26572-26578 NN denotes groups
T10950 26579-26580 -LRB- denotes [
T10951 26580-26582 CD denotes 16
T10952 26582-26583 -RRB- denotes ]
T10953 26583-26584 -COMMA- denotes ,
T10954 26585-26586 -LRB- denotes [
T10955 26586-26588 CD denotes 22
T10956 26588-26589 -RRB- denotes ]
T10957 26590-26597 VB denotes support
T10958 26598-26601 DT denotes the
T10959 26602-26613 NN denotes possibility
T10960 26614-26618 IN denotes that
T10961 26619-26623 NN denotes HOIP
T10962 26624-26626 VB denotes is
T10963 26627-26636 JJ denotes important
T10964 26637-26639 IN denotes in
T10965 26640-26644 JJ denotes many
T10966 26645-26654 NN denotes signaling
T10967 26655-26663 NN denotes pathways
T10968 26664-26666 IN denotes in
T10969 26667-26672 WDT denotes which
T10970 26673-26678 NN denotes TRAF2
T10971 26679-26681 CC denotes or
T10972 26682-26687 NN denotes TRAF6
T10973 26688-26691 VB denotes are
T10974 26692-26700 VB denotes involved
T10975 26700-26701 -COMMA- denotes ,
T10976 26702-26711 VB denotes including
T10977 26712-26717 DT denotes those
T10978 26718-26728 VB denotes associated
T10979 26729-26733 IN denotes with
T10980 26734-26741 JJ denotes various
T10981 26742-26749 NN denotes members
T10982 26750-26752 IN denotes of
T10983 26753-26756 DT denotes the
T10984 26757-26760 NN denotes TNF
T10985 26761-26769 NN denotes receptor
T10986 26770-26781 NN denotes superfamily
T10987 26782-26785 CC denotes and
T10988 26786-26789 DT denotes the
T10989 26790-26799 JJ denotes Toll-like
T10990 26800-26809 NN denotes receptors
T10991 26811-26814 DT denotes The
T10992 26815-26824 JJ denotes potential
T10993 26825-26835 NN denotes importance
T10994 26836-26838 IN denotes of
T10995 26839-26843 NN denotes HOIP
T10996 26844-26846 IN denotes in
T10997 26847-26853 JJ denotes immune
T10998 26854-26862 NN denotes function
T10999 26863-26866 CC denotes and
T11000 26867-26871 NN denotes TNFR
T11001 26872-26878 NN denotes family
T11002 26879-26888 NN denotes signaling
T11003 26889-26891 VB denotes is
T11004 26892-26899 RB denotes further
T11005 26900-26909 VB denotes supported
T11006 26910-26912 IN denotes by
T11007 26913-26916 DT denotes the
T11008 26917-26923 JJ denotes recent
T11009 26924-26933 NN denotes discovery
T11010 26934-26938 IN denotes that
T11011 26939-26943 NN denotes HOIP
T11012 26944-26953 VB denotes interacts
T11013 26954-26958 IN denotes with
T11014 26959-26960 DT denotes a
T11015 26961-26968 NN denotes protein
T11016 26969-26974 VB denotes known
T11017 26975-26977 IN denotes as
T11018 26978-26985 NN denotes SHARPIN
T11019 26986-26987 -LRB- denotes (
T11020 26987-27003 JJ denotes SHANK-associated
T11021 27004-27006 NN denotes RH
T11022 27007-27013 NN denotes domain
T11023 27014-27025 VB denotes interacting
T11024 27026-27033 NN denotes protein
T11025 27034-27036 IN denotes in
T11026 27037-27049 JJ denotes postsynaptic
T11027 27050-27057 NN denotes density
T11028 27057-27058 -RRB- denotes )
T11029 27058-27059 -COMMA- denotes ,
T11030 27060-27065 WDT denotes which
T11031 27066-27073 VB denotes appears
T11032 27074-27081 JJ denotes capable
T11033 27082-27084 IN denotes of
T11034 27085-27092 VB denotes working
T11035 27093-27101 RB denotes together
T11036 27102-27106 IN denotes with
T11037 27107-27111 NN denotes HOIP
T11038 27112-27115 CC denotes and
T11039 27116-27120 NN denotes HOIL
T11040 27121-27123 TO denotes to
T11041 27124-27131 VB denotes mediate
T11042 27132-27135 DT denotes the
T11043 27136-27144 NN denotes assembly
T11044 27145-27147 IN denotes of
T11045 27148-27154 JJ denotes linear
T11046 27155-27168 NN denotes polyubiquitin
T11047 27169-27170 -LRB- denotes [
T11048 27170-27172 CD denotes 14
T11049 27172-27173 -RRB- denotes ]
T11050 27173-27174 -COMMA- denotes ,
T11051 27175-27176 -LRB- denotes [
T11052 27176-27178 CD denotes 23
T11053 27178-27179 -RRB- denotes ]
T11054 27179-27180 -COMMA- denotes ,
T11055 27181-27182 -LRB- denotes [
T11056 27182-27184 CD denotes 24
T11057 27184-27185 -RRB- denotes ]
T11058 27187-27200 RB denotes Interestingly
T11059 27200-27201 -COMMA- denotes ,
T11060 27202-27206 NN denotes mice
T11061 27207-27211 IN denotes with
T11062 27212-27213 DT denotes a
T11063 27214-27225 JJ denotes spontaneous
T11064 27226-27234 NN denotes mutation
T11065 27235-27237 IN denotes in
T11066 27238-27241 DT denotes the
T11067 27242-27246 NN denotes gene
T11068 27247-27255 VB denotes encoding
T11069 27256-27263 NN denotes SHARPIN
T11070 27264-27265 -LRB- denotes (
T11071 27265-27272 JJ denotes chronic
T11072 27273-27286 JJ denotes proliferative
T11073 27287-27297 NN denotes dermatitis
T11074 27298-27299 -LRB- denotes (
T11075 27299-27303 NN denotes cpdm
T11076 27303-27304 -RRB- denotes )
T11077 27305-27309 NN denotes mice
T11078 27309-27310 -RRB- denotes )
T11079 27311-27318 VB denotes exhibit
T11080 27319-27326 JJ denotes chronic
T11081 27327-27339 NN denotes inflammation
T11082 27340-27342 IN denotes of
T11083 27343-27346 DT denotes the
T11084 27347-27351 NN denotes skin
T11085 27352-27355 CC denotes and
T11086 27356-27364 JJ denotes internal
T11087 27365-27371 NN denotes organs
T11088 27371-27372 -COMMA- denotes ,
T11089 27373-27382 JJ denotes defective
T11090 27383-27394 NN denotes development
T11091 27395-27397 IN denotes of
T11092 27398-27407 JJ denotes secondary
T11093 27408-27416 JJ denotes lymphoid
T11094 27417-27423 NN denotes tissue
T11095 27423-27424 -COMMA- denotes ,
T11096 27425-27428 CC denotes and
T11097 27429-27436 NN denotes defects
T11098 27437-27439 IN denotes in
T11099 27440-27443 DT denotes the
T11100 27444-27454 NN denotes production
T11101 27455-27457 IN denotes of
T11102 27458-27466 VB denotes switched
T11103 27467-27481 NN denotes immunoglobulin
T11104 27482-27490 NN denotes isotypes
T11105 27491-27492 -LRB- denotes [
T11106 27492-27494 CD denotes 17
T11107 27494-27495 -RRB- denotes ]
T11108 27495-27496 -COMMA- denotes ,
T11109 27497-27498 -LRB- denotes [
T11110 27498-27500 CD denotes 25
T11111 27500-27501 -RRB- denotes ]
T11112 27503-27506 DT denotes The
T11113 27507-27517 RB denotes apparently
T11114 27518-27526 JJ denotes intimate
T11115 27527-27537 JJ denotes functional
T11116 27538-27542 NN denotes link
T11117 27543-27550 IN denotes between
T11118 27551-27558 NN denotes SHARPIN
T11119 27559-27562 CC denotes and
T11120 27563-27567 NN denotes HOIP
T11121 27568-27576 RB denotes strongly
T11122 27577-27585 VB denotes suggests
T11123 27586-27590 IN denotes that
T11124 27591-27593 IN denotes at
T11125 27594-27599 JJ denotes least
T11126 27600-27604 NN denotes part
T11127 27605-27607 IN denotes of
T11128 27608-27611 DT denotes the
T11129 27612-27616 NN denotes cpdm
T11130 27617-27626 NN denotes phenotype
T11131 27627-27632 VB denotes stems
T11132 27633-27637 IN denotes from
T11133 27638-27645 NN denotes defects
T11134 27646-27648 IN denotes in
T11135 27649-27652 DT denotes the
T11136 27653-27663 NN denotes regulation
T11137 27664-27666 CC denotes or
T11138 27667-27675 NN denotes function
T11139 27676-27678 IN denotes of
T11140 27679-27683 NN denotes HOIP
T11983 27709-27713 NN denotes Cell
T11984 27714-27719 NN denotes lines
T11985 27720-27723 DT denotes The
T11986 27724-27729 NN denotes mouse
T11987 27730-27731 NN denotes B
T11988 27732-27736 NN denotes cell
T11989 27737-27741 NN denotes line
T11990 27742-27748 NN denotes A20.2J
T11991 27749-27752 VB denotes has
T11992 27753-27757 VB denotes been
T11993 27758-27768 RB denotes previously
T11994 27769-27778 VB denotes described
T11995 27779-27780 -LRB- denotes [
T11996 27780-27782 CD denotes 26
T11997 27782-27783 -RRB- denotes ]
T11998 27783-27784 -COMMA- denotes ,
T11999 27785-27786 -LRB- denotes [
T12000 27786-27788 CD denotes 27
T12001 27788-27789 -RRB- denotes ]
T12002 27791-27796 NN denotes Cells
T12003 27797-27801 VB denotes were
T12004 27802-27807 VB denotes grown
T12005 27808-27810 IN denotes in
T12006 27811-27815 NN denotes RPMI
T12007 27816-27820 CD denotes 1640
T12008 27820-27821 -COMMA- denotes ,
T12009 27822-27824 CD denotes 10
T12010 27824-27825 NN denotes %
T12011 27826-27829 NN denotes FCS
T12012 27829-27830 -COMMA- denotes ,
T12013 27831-27833 CD denotes 10
T12014 27834-27836 NN denotes µM
T12015 27837-27841 NN denotes 2-ME
T12016 27841-27842 -COMMA- denotes ,
T12017 27843-27844 CD denotes 2
T12018 27845-27847 NN denotes mM
T12019 27848-27859 NN denotes L-glutamine
T12020 27859-27860 -COMMA- denotes ,
T12021 27861-27864 CC denotes and
T12022 27865-27876 NN denotes antibiotics
T12023 27878-27882 NN denotes HI-5
T12024 27883-27889 NN denotes insect
T12025 27890-27895 NN denotes cells
T12026 27896-27897 -LRB- denotes (
T12027 27897-27907 NN denotes Invitrogen
T12028 27907-27908 -RRB- denotes )
T12029 27909-27919 VB denotes expressing
T12030 27920-27925 NN denotes CD154
T12031 27926-27930 VB denotes have
T12032 27931-27935 VB denotes been
T12033 27936-27945 VB denotes described
T12034 27946-27947 -LRB- denotes [
T12035 27947-27949 CD denotes 28
T12036 27949-27950 -RRB- denotes ]
T12163 27953-27963 NN denotes Antibodies
T12164 27964-27969 NN denotes Mouse
T12165 27970-27980 JJ denotes anti-birch
T12166 27981-27989 NN denotes profilin
T12167 27990-27998 NN denotes antibody
T12168 27999-28000 -LRB- denotes (
T12169 28000-28003 NN denotes 4A6
T12170 28004-28005 -LRB- denotes [
T12171 28005-28007 CD denotes 29
T12172 28007-28008 -RRB- denotes ]
T12173 28008-28009 -RRB- denotes )
T12174 28009-28010 -COMMA- denotes ,
T12175 28011-28014 NN denotes rat
T12176 28015-28025 JJ denotes anti-mouse
T12177 28026-28030 NN denotes CD40
T12178 28031-28032 -LRB- denotes (
T12179 28032-28036 CD denotes 1C10
T12180 28037-28038 -LRB- denotes [
T12181 28038-28040 CD denotes 30
T12182 28040-28041 -RRB- denotes ]
T12183 28041-28042 -RRB- denotes )
T12184 28043-28046 CC denotes and
T12185 28047-28048 DT denotes a
T12186 28049-28052 NN denotes rat
T12187 28053-28060 NN denotes isotype
T12188 28061-28068 NN denotes control
T12189 28069-28077 NN denotes antibody
T12190 28078-28079 -LRB- denotes (
T12191 28079-28084 NN denotes mAb72
T12192 28084-28085 -COMMA- denotes ,
T12193 28086-28099 NNP denotes Developmental
T12194 28100-28107 NNP denotes Studies
T12195 28108-28117 NNP denotes Hybridoma
T12196 28118-28122 NNP denotes Bank
T12197 28122-28123 -COMMA- denotes ,
T12198 28124-28134 NNP denotes University
T12199 28135-28137 IN denotes of
T12200 28138-28142 NNP denotes Iowa
T12201 28142-28143 -COMMA- denotes ,
T12202 28144-28148 NNP denotes Iowa
T12203 28149-28153 NNP denotes City
T12204 28153-28154 -COMMA- denotes ,
T12205 28155-28157 NN denotes IA
T12206 28157-28158 -RRB- denotes )
T12207 28159-28163 VB denotes were
T12208 28164-28172 VB denotes isolated
T12209 28173-28177 IN denotes from
T12210 28178-28187 NN denotes hybridoma
T12211 28188-28200 NN denotes supernatants
T12212 28202-28214 JJ denotes FITC-labeled
T12213 28215-28225 JJ denotes anti-mouse
T12214 28226-28230 NN denotes CD80
T12215 28231-28234 CC denotes and
T12216 28235-28237 DT denotes an
T12217 28238-28245 NN denotes isotype
T12218 28246-28253 NN denotes control
T12219 28254-28256 NN denotes Ab
T12220 28257-28261 VB denotes were
T12221 28262-28266 IN denotes from
T12222 28267-28279 NN denotes eBiosciences
T12223 28281-28287 NN denotes Rabbit
T12224 28288-28297 JJ denotes anti-HOIP
T12225 28298-28300 NN denotes Ab
T12226 28301-28302 -LRB- denotes [
T12227 28302-28303 CD denotes 6
T12228 28303-28304 -RRB- denotes ]
T12229 28305-28308 VB denotes was
T12230 28309-28312 DT denotes the
T12231 28313-28317 NN denotes kind
T12232 28318-28322 NN denotes gift
T12233 28323-28325 IN denotes of
T12234 28326-28329 NNP denotes Dr.
T12235 28330-28335 NNP denotes Betty
T12236 28336-28342 NNP denotes Eipper
T12237 28343-28344 -LRB- denotes (
T12238 28344-28354 NNP denotes University
T12239 28355-28357 IN denotes of
T12240 28358-28369 NNP denotes Connecticut
T12241 28370-28376 NNP denotes Health
T12242 28377-28383 NNP denotes Center
T12243 28383-28384 -COMMA- denotes ,
T12244 28385-28395 NNP denotes Farmington
T12245 28395-28396 -COMMA- denotes ,
T12246 28397-28408 NNP denotes Connecticut
T12247 28408-28409 -RRB- denotes )
T12248 28411-28415 NNP denotes Goat
T12249 28416-28424 JJ denotes anti-rat
T12250 28425-28428 NN denotes IgG
T12251 28428-28429 -COMMA- denotes ,
T12252 28430-28433 CC denotes and
T12253 28434-28445 JJ denotes HRP-labeled
T12254 28446-28455 JJ denotes secondary
T12255 28456-28459 NN denotes Abs
T12256 28460-28464 VB denotes were
T12257 28465-28469 IN denotes from
T12258 28470-28477 NNP denotes Jackson
T12259 28478-28492 NNP denotes ImmunoResearch
T12260 28493-28505 NNP denotes Laboratories
T12261 28505-28506 -COMMA- denotes ,
T12262 28507-28511 NNP denotes Inc.
T12263 28512-28515 DT denotes All
T12264 28516-28521 JJ denotes other
T12265 28522-28532 NN denotes antibodies
T12266 28533-28537 VB denotes used
T12267 28538-28542 VB denotes were
T12268 28543-28552 VB denotes described
T12269 28553-28563 RB denotes previously
T12270 28564-28565 -LRB- denotes [
T12271 28565-28566 CD denotes 6
T12272 28566-28567 -RRB- denotes ]
T12597 28570-28577 JJ denotes Somatic
T12598 28578-28582 NN denotes cell
T12599 28583-28587 NN denotes gene
T12600 28588-28597 VB denotes targeting
T12601 28598-28601 DT denotes The
T12602 28602-28612 NN denotes generation
T12603 28613-28615 IN denotes of
T12604 28616-28630 JJ denotes HOIP-deficient
T12605 28631-28636 NN denotes cells
T12606 28637-28640 VB denotes was
T12607 28641-28653 VB denotes accomplished
T12608 28654-28659 VB denotes using
T12609 28660-28661 DT denotes a
T12610 28662-28672 JJ denotes homologous
T12611 28673-28686 NN denotes recombination
T12612 28687-28695 NN denotes approach
T12613 28696-28705 VB denotes described
T12614 28706-28716 RB denotes previously
T12615 28717-28718 -LRB- denotes [
T12616 28718-28719 CD denotes 7
T12617 28719-28720 -RRB- denotes ]
T12618 28720-28721 -COMMA- denotes ,
T12619 28722-28723 -LRB- denotes [
T12620 28723-28724 CD denotes 8
T12621 28724-28725 -RRB- denotes ]
T12622 28727-28735 NN denotes Segments
T12623 28736-28738 IN denotes of
T12624 28739-28744 NN denotes Rnf31
T12625 28745-28749 NN denotes gene
T12626 28750-28758 NN denotes sequence
T12627 28759-28763 VB denotes used
T12628 28764-28766 IN denotes in
T12629 28767-28770 DT denotes the
T12630 28771-28780 VB denotes targeting
T12631 28781-28790 NN denotes construct
T12632 28791-28792 -LRB- denotes (
T12633 28792-28796 NN denotes Fig.
T12634 28797-28799 NN denotes 1A
T12635 28799-28800 -RRB- denotes )
T12636 28801-28805 VB denotes were
T12637 28806-28815 VB denotes amplified
T12638 28816-28818 IN denotes by
T12639 28819-28822 NN denotes PCR
T12640 28823-28827 IN denotes from
T12641 28828-28834 NN denotes A20.2J
T12642 28835-28842 JJ denotes genomic
T12643 28843-28846 NN denotes DNA
T12644 28848-28851 DT denotes The
T12645 28852-28867 NN denotes oligonucleotide
T12646 28868-28875 NN denotes primers
T12647 28876-28880 VB denotes used
T12648 28881-28883 TO denotes to
T12649 28884-28892 VB denotes generate
T12650 28893-28896 DT denotes the
T12651 28897-28899 NN denotes 5′
T12652 28900-28905 NN denotes flank
T12653 28906-28907 -LRB- denotes (
T12654 28907-28911 CD denotes 1224
T12655 28912-28914 NN denotes bp
T12656 28914-28915 -RRB- denotes )
T12657 28916-28920 VB denotes were
T12658 28921-28955 JJ denotes 5′-ttttctagagcggtggcttaagtgaccc-3′
T12659 28956-28959 CC denotes and
T12660 28960-28997 JJ denotes 5′-tattctagatgcagcatctgagaaagcaagc-3′
T12661 28999-29002 DT denotes The
T12662 29003-29005 NN denotes 3′
T12663 29006-29011 NN denotes flank
T12664 29012-29013 -LRB- denotes (
T12665 29013-29017 CD denotes 6032
T12666 29018-29020 NN denotes bp
T12667 29020-29021 -RRB- denotes )
T12668 29022-29029 NN denotes primers
T12669 29030-29034 VB denotes were
T12670 29035-29080 JJ denotes 5′-aaaaccggtgtatgcttctttacgggagaaaaatattag-3′
T12671 29081-29084 CC denotes and
T12672 29085-29124 JJ denotes 5′-tataccggtatgaagccaaaggaacactgagag-3′
T12673 29126-29137 NN denotes Restriction
T12674 29138-29150 NN denotes endonuclease
T12675 29151-29156 NN denotes sites
T12676 29157-29159 IN denotes in
T12677 29160-29163 DT denotes the
T12678 29164-29179 NN denotes oligonucleotide
T12679 29180-29187 NN denotes primers
T12680 29188-29195 VB denotes allowed
T12681 29196-29205 NN denotes insertion
T12682 29206-29208 IN denotes of
T12683 29209-29212 DT denotes the
T12684 29213-29216 NN denotes PCR
T12685 29217-29225 NN denotes products
T12686 29226-29230 IN denotes into
T12687 29231-29234 DT denotes the
T12688 29235-29244 NN denotes targeting
T12689 29245-29251 NN denotes vector
T12690 29253-29259 NN denotes A20.2J
T12691 29260-29265 NN denotes cells
T12692 29266-29270 VB denotes were
T12693 29271-29282 VB denotes transfected
T12694 29283-29284 -LRB- denotes (
T12695 29284-29286 IN denotes by
T12696 29287-29302 NN denotes electroporation
T12697 29303-29304 -LRB- denotes [
T12698 29304-29305 CD denotes 7
T12699 29305-29306 -RRB- denotes ]
T12700 29306-29307 -RRB- denotes )
T12701 29308-29312 IN denotes with
T12702 29313-29316 DT denotes the
T12703 29317-29326 VB denotes targeting
T12704 29327-29336 NN denotes construct
T12705 29337-29340 CC denotes and
T12706 29341-29350 VB denotes subcloned
T12707 29351-29353 IN denotes in
T12708 29354-29360 NN denotes medium
T12709 29361-29371 VB denotes containing
T12710 29372-29375 CD denotes 600
T12711 29376-29381 NN denotes µg/ml
T12712 29382-29386 NN denotes G418
T12713 29387-29394 NN denotes sulfate
T12714 29396-29406 JJ denotes Homologous
T12715 29407-29420 NN denotes recombination
T12716 29421-29423 IN denotes in
T12717 29424-29438 JJ denotes G418-resistant
T12718 29439-29445 NN denotes clones
T12719 29446-29449 VB denotes was
T12720 29450-29458 VB denotes detected
T12721 29459-29461 IN denotes by
T12722 29462-29465 NN denotes PCR
T12723 29466-29468 IN denotes of
T12724 29469-29476 JJ denotes genomic
T12725 29477-29480 NN denotes DNA
T12726 29480-29481 -COMMA- denotes ,
T12727 29482-29484 IN denotes as
T12728 29485-29494 VB denotes described
T12729 29495-29496 -LRB- denotes [
T12730 29496-29497 CD denotes 7
T12731 29497-29498 -RRB- denotes ]
T12732 29500-29515 NN denotes Oligonucleotide
T12733 29516-29523 NN denotes primers
T12734 29524-29528 VB denotes used
T12735 29529-29532 IN denotes for
T12736 29533-29542 NN denotes screening
T12737 29543-29547 VB denotes were
T12738 29548-29580 JJ denotes 5′-cttcctgatctcagctttaccgtcac-3′
T12739 29581-29582 -LRB- denotes (
T12740 29582-29592 JJ denotes homologous
T12741 29593-29595 TO denotes to
T12742 29596-29603 JJ denotes genomic
T12743 29604-29612 NN denotes sequence
T12744 29612-29613 -COLON- denotes ;
T12745 29614-29625 JJ denotes approximate
T12746 29626-29634 NN denotes position
T12747 29635-29640 VB denotes noted
T12748 29641-29643 IN denotes in
T12749 29644-29648 NNP denotes Fig.
T12750 29649-29650 CD denotes 1
T12751 29650-29651 -RRB- denotes )
T12752 29652-29655 CC denotes and
T12753 29656-29683 JJ denotes 5′-caatccatcttgttcagccat-3′
T12754 29684-29685 -LRB- denotes (
T12755 29685-29695 JJ denotes homologous
T12756 29696-29698 TO denotes to
T12757 29699-29707 NN denotes sequence
T12758 29708-29710 IN denotes in
T12759 29711-29715 NN denotes NeoR
T12760 29715-29716 -RRB- denotes )
T12761 29718-29724 NN denotes Clones
T12762 29725-29727 IN denotes in
T12763 29728-29733 WDT denotes which
T12764 29734-29737 CD denotes one
T12765 29738-29742 NN denotes copy
T12766 29743-29745 IN denotes of
T12767 29746-29751 NN denotes Rnf31
T12768 29752-29755 VB denotes had
T12769 29756-29760 VB denotes been
T12770 29761-29770 VB denotes disrupted
T12771 29771-29775 VB denotes were
T12772 29776-29787 RB denotes transiently
T12773 29788-29799 VB denotes transfected
T12774 29800-29804 IN denotes with
T12775 29805-29807 DT denotes an
T12776 29808-29818 NN denotes expression
T12777 29819-29826 NN denotes plasmid
T12778 29827-29835 VB denotes encoding
T12779 29836-29839 NNP denotes Cre
T12780 29839-29840 -COMMA- denotes ,
T12781 29841-29843 IN denotes in
T12782 29844-29849 NN denotes order
T12783 29850-29852 TO denotes to
T12784 29853-29859 VB denotes remove
T12785 29860-29864 NN denotes NeoR
T12786 29866-29880 JJ denotes G418-sensitive
T12787 29881-29890 NN denotes subclones
T12788 29891-29895 VB denotes were
T12789 29896-29905 VB denotes subjected
T12790 29906-29908 TO denotes to
T12791 29909-29910 DT denotes a
T12792 29911-29917 JJ denotes second
T12793 29918-29923 NN denotes round
T12794 29924-29926 IN denotes of
T12795 29927-29936 VB denotes targeting
T12796 29937-29939 TO denotes to
T12797 29940-29947 VB denotes disrupt
T12798 29948-29951 DT denotes the
T12799 29952-29961 VB denotes remaining
T12800 29962-29966 NN denotes copy
T12801 29967-29969 IN denotes of
T12802 29970-29975 NN denotes Rnf31
T12803 29977-29991 JJ denotes G418-resistant
T12804 29992-29998 NN denotes clones
T12805 29999-30003 VB denotes were
T12806 30004-30010 VB denotes tested
T12807 30011-30014 IN denotes for
T12808 30015-30025 JJ denotes homologous
T12809 30026-30039 NN denotes recombination
T12810 30040-30042 IN denotes by
T12811 30043-30046 NN denotes PCR
T12812 30047-30050 CC denotes and
T12813 30051-30055 NN denotes HOIP
T12814 30056-30063 NN denotes protein
T12815 30064-30074 NN denotes expression
T12816 30075-30077 IN denotes by
T12817 30078-30085 JJ denotes Western
T12818 30086-30090 NN denotes blot
T13106 30093-30103 JJ denotes Retroviral
T13107 30104-30116 NN denotes transduction
T13108 30117-30118 DT denotes A
T13109 30119-30129 JJ denotes retroviral
T13110 30130-30136 NN denotes vector
T13111 30137-30138 -LRB- denotes (
T13112 30138-30142 NN denotes pMIP
T13113 30142-30143 -RRB- denotes )
T13114 30144-30147 VB denotes was
T13115 30148-30152 VB denotes used
T13116 30153-30156 IN denotes for
T13117 30157-30160 DT denotes the
T13118 30161-30167 JJ denotes stable
T13119 30168-30180 NN denotes transduction
T13120 30181-30183 IN denotes of
T13121 30184-30189 NN denotes cells
T13122 30190-30194 IN denotes with
T13123 30195-30199 NN denotes HOIP
T13124 30200-30203 CC denotes and
T13125 30204-30208 NN denotes IKKγ
T13126 30209-30213 NN denotes cDNA
T13127 30214-30225 NN denotes constructs.
T13128 30226-30230 NN denotes pMIP
T13129 30231-30234 VB denotes was
T13130 30235-30246 VB denotes constructed
T13131 30247-30249 IN denotes by
T13132 30250-30259 VB denotes replacing
T13133 30260-30263 DT denotes the
T13134 30264-30268 NN denotes IRES
T13135 30269-30272 CC denotes and
T13136 30273-30276 NN denotes GFP
T13137 30277-30285 VB denotes encoding
T13138 30286-30295 NN denotes sequences
T13139 30296-30298 IN denotes in
T13140 30299-30303 NN denotes pMIG
T13141 30304-30305 -LRB- denotes [
T13142 30305-30307 CD denotes 31
T13143 30307-30308 -RRB- denotes ]
T13144 30309-30313 IN denotes with
T13145 30314-30317 DT denotes the
T13146 30318-30322 NN denotes IRES
T13147 30323-30326 CC denotes and
T13148 30327-30336 NN denotes puromycin
T13149 30337-30347 NN denotes resistance
T13150 30348-30352 NN denotes gene
T13151 30353-30357 IN denotes from
T13152 30358-30368 NN denotes pIRESpuro2
T13153 30369-30370 -LRB- denotes (
T13154 30370-30378 NN denotes Clontech
T13155 30378-30379 -RRB- denotes )
T13156 30381-30392 JJ denotes FLAG-tagged
T13157 30393-30398 NN denotes mouse
T13158 30399-30403 NN denotes HOIP
T13159 30404-30408 NN denotes cDNA
T13160 30409-30412 VB denotes was
T13161 30413-30421 VB denotes inserted
T13162 30422-30426 IN denotes into
T13163 30427-30430 DT denotes the
T13164 30431-30439 JJ denotes multiple
T13165 30440-30447 NN denotes cloning
T13166 30448-30452 NN denotes site
T13167 30453-30455 IN denotes of
T13168 30456-30460 NN denotes pMIP
T13169 30462-30463 DT denotes A
T13170 30464-30468 NN denotes pMIP
T13171 30469-30478 NN denotes construct
T13172 30479-30487 VB denotes encoding
T13173 30488-30493 NN denotes mouse
T13174 30494-30498 NN denotes IKKγ
T13175 30499-30503 IN denotes with
T13176 30504-30506 DT denotes an
T13177 30507-30512 JJ denotes amino
T13178 30513-30521 JJ denotes terminal
T13179 30522-30527 NN denotes birch
T13180 30528-30536 NN denotes profilin
T13181 30537-30544 NN denotes peptide
T13182 30545-30546 -LRB- denotes (
T13183 30546-30548 NN denotes BP
T13184 30548-30549 -RRB- denotes )
T13185 30549-30553 NN denotes -tag
T13186 30554-30557 VB denotes was
T13187 30558-30562 RB denotes also
T13188 30563-30571 VB denotes prepared
T13189 30573-30583 JJ denotes Retroviral
T13190 30584-30593 NN denotes particles
T13191 30594-30598 VB denotes were
T13192 30599-30608 VB denotes generated
T13193 30609-30611 IN denotes by
T13194 30612-30621 JJ denotes transient
T13195 30622-30634 NN denotes transfection
T13196 30635-30637 IN denotes of
T13197 30638-30642 NN denotes 293T
T13198 30643-30648 NN denotes cells
T13199 30649-30653 IN denotes with
T13200 30654-30658 NN denotes pMIP
T13201 30658-30659 -COMMA- denotes ,
T13202 30660-30669 NN denotes pMIP-HOIP
T13203 30669-30670 -COMMA- denotes ,
T13204 30671-30673 CC denotes or
T13205 30674-30683 NN denotes pMIP-IKKγ
T13206 30684-30687 CC denotes and
T13207 30688-30691 DT denotes the
T13208 30692-30701 NN denotes packaging
T13209 30702-30708 NN denotes vector
T13210 30709-30716 NN denotes pCL-Eco
T13211 30717-30718 -LRB- denotes [
T13212 30718-30720 CD denotes 32
T13213 30720-30721 -RRB- denotes ]
T13214 30723-30726 CD denotes Two
T13215 30727-30731 NN denotes days
T13216 30732-30737 IN denotes after
T13217 30738-30750 NN denotes transfection
T13218 30750-30751 -COMMA- denotes ,
T13219 30752-30759 NN denotes culture
T13220 30760-30772 NN denotes supernatants
T13221 30773-30777 VB denotes were
T13222 30778-30787 VB denotes harvested
T13223 30788-30791 CC denotes and
T13224 30792-30800 VB denotes filtered
T13225 30802-30808 NN denotes A20.2J
T13226 30809-30811 CC denotes or
T13227 30812-30826 JJ denotes HOIP-deficient
T13228 30827-30832 NN denotes cells
T13229 30833-30837 VB denotes were
T13230 30838-30843 VB denotes added
T13231 30844-30846 TO denotes to
T13232 30847-30854 JJ denotes 24-well
T13233 30855-30861 NN denotes plates
T13234 30862-30863 -LRB- denotes (
T13235 30863-30870 CD denotes 2.5×105
T13236 30871-30881 NN denotes cells/well
T13237 30881-30882 -RRB- denotes )
T13238 30883-30887 IN denotes with
T13239 30888-30889 CD denotes 1
T13240 30890-30892 NN denotes ml
T13241 30893-30909 JJ denotes virus-containing
T13242 30910-30921 NN denotes supernatant
T13243 30922-30925 CC denotes and
T13244 30926-30927 CD denotes 8
T13245 30928-30933 NN denotes µg/ml
T13246 30934-30943 NN denotes polybrene
T13247 30945-30951 NN denotes Plates
T13248 30952-30956 VB denotes were
T13249 30957-30968 VB denotes centrifuged
T13250 30969-30971 IN denotes at
T13251 30972-30975 CD denotes 500
T13252 30976-30977 SYM denotes x
T13253 30978-30979 NN denotes g
T13254 30980-30983 IN denotes for
T13255 30984-30985 CD denotes 2
T13256 30986-30989 NN denotes hrs
T13257 30990-30992 IN denotes at
T13258 30993-30997 NN denotes room
T13259 30998-31009 NN denotes temperature
T13260 31011-31016 NN denotes Cells
T13261 31017-31021 VB denotes were
T13262 31022-31030 VB denotes cultured
T13263 31031-31033 IN denotes in
T13264 31034-31035 NN denotes B
T13265 31036-31040 NN denotes cell
T13266 31041-31048 NN denotes culture
T13267 31049-31055 NN denotes medium
T13268 31056-31059 IN denotes for
T13269 31060-31062 CD denotes 48
T13270 31063-31066 NN denotes hrs
T13271 31066-31067 -COMMA- denotes ,
T13272 31068-31073 IN denotes after
T13273 31074-31079 WDT denotes which
T13274 31080-31083 DT denotes the
T13275 31084-31094 VB denotes transduced
T13276 31095-31100 NN denotes cells
T13277 31101-31105 VB denotes were
T13278 31106-31114 VB denotes selected
T13279 31115-31117 IN denotes in
T13280 31118-31119 CD denotes 3
T13281 31120-31125 NN denotes µg/ml
T13282 31126-31135 NN denotes puromycin
T13427 31138-31142 NN denotes CD80
T13428 31143-31155 NN denotes upregulation
T13429 31156-31159 CC denotes and
T13430 31160-31164 NN denotes flow
T13431 31165-31174 NN denotes cytometry
T13432 31175-31180 NN denotes Cells
T13433 31181-31182 -LRB- denotes (
T13434 31182-31187 CD denotes 5×104
T13435 31188-31190 IN denotes in
T13436 31191-31192 CD denotes 2
T13437 31193-31195 NN denotes ml
T13438 31195-31196 -RRB- denotes )
T13439 31197-31201 VB denotes were
T13440 31202-31210 VB denotes cultured
T13441 31211-31213 IN denotes in
T13442 31214-31221 JJ denotes 24-well
T13443 31222-31228 NN denotes plates
T13444 31229-31232 IN denotes for
T13445 31233-31234 CD denotes 3
T13446 31235-31239 NN denotes days
T13447 31240-31244 IN denotes with
T13448 31245-31246 CD denotes 5
T13449 31247-31252 NN denotes µg/ml
T13450 31253-31262 NN denotes anti-CD40
T13451 31263-31265 CC denotes or
T13452 31266-31268 DT denotes an
T13453 31269-31276 NN denotes isotype
T13454 31277-31284 NN denotes control
T13455 31285-31293 NN denotes antibody
T13456 31295-31300 NN denotes Cells
T13457 31301-31305 VB denotes were
T13458 31306-31313 VB denotes stained
T13459 31314-31317 IN denotes for
T13460 31318-31322 NN denotes flow
T13461 31323-31332 NN denotes cytometry
T13462 31333-31337 IN denotes with
T13463 31338-31352 NN denotes FITC-anti-CD80
T13464 31353-31355 CC denotes or
T13465 31356-31358 DT denotes an
T13466 31359-31370 JJ denotes appropriate
T13467 31371-31378 NN denotes control
T13468 31379-31387 NN denotes antibody
T13469 31388-31390 RB denotes as
T13470 31391-31400 VB denotes described
T13471 31401-31402 -LRB- denotes [
T13472 31402-31404 CD denotes 28
T13473 31404-31405 -RRB- denotes ]
T13474 31407-31411 NN denotes Data
T13475 31412-31420 VB denotes acquired
T13476 31421-31425 IN denotes with
T13477 31426-31427 DT denotes a
T13478 31428-31435 NN denotes FACScan
T13479 31436-31440 NN denotes flow
T13480 31441-31450 NN denotes cytometer
T13481 31451-31452 -LRB- denotes (
T13482 31452-31454 NN denotes BD
T13483 31455-31466 NNP denotes Biosciences
T13484 31466-31467 -RRB- denotes )
T13485 31468-31472 VB denotes were
T13486 31473-31481 VB denotes analyzed
T13487 31482-31486 IN denotes with
T13488 31487-31493 NNP denotes WinMDI
T13489 31494-31497 CD denotes 2.8
T13490 31498-31499 -LRB- denotes (
T13491 31499-31506 NNP denotes Scripps
T13492 31507-31515 NNP denotes Research
T13493 31516-31525 NNP denotes Institute
T13494 31525-31526 -COMMA- denotes ,
T13495 31527-31530 NNP denotes San
T13496 31531-31536 NNP denotes Diego
T13497 31536-31537 -COMMA- denotes ,
T13498 31538-31540 NNP denotes CA
T13499 31540-31541 -RRB- denotes )
T13684 31544-31547 NN denotes GLε
T13685 31548-31558 NN denotes transcript
T13686 31559-31564 NN denotes assay
T13687 31565-31568 DT denotes The
T13688 31569-31583 JJ denotes CD40-simulated
T13689 31584-31594 NN denotes activation
T13690 31595-31597 IN denotes of
T13691 31598-31601 NN denotes GLε
T13692 31602-31615 NN denotes transcription
T13693 31616-31619 VB denotes was
T13694 31620-31629 VB denotes evaluated
T13695 31630-31632 IN denotes as
T13696 31633-31643 RB denotes previously
T13697 31644-31652 VB denotes reported
T13698 31653-31654 -LRB- denotes [
T13699 31654-31656 CD denotes 33
T13700 31656-31657 -RRB- denotes ]
T13701 31659-31666 NNP denotes Briefly
T13702 31666-31667 -COMMA- denotes ,
T13703 31668-31673 CD denotes 1×106
T13704 31674-31679 NN denotes cells
T13705 31680-31684 VB denotes were
T13706 31685-31695 VB denotes stimulated
T13707 31696-31705 RB denotes overnight
T13708 31706-31710 IN denotes with
T13709 31711-31720 JJ denotes anti-CD40
T13710 31721-31729 NN denotes antibody
T13711 31730-31731 -LRB- denotes (
T13712 31731-31733 CD denotes 10
T13713 31734-31739 NN denotes µg/ml
T13714 31739-31740 -RRB- denotes )
T13715 31741-31743 CC denotes or
T13716 31744-31746 DT denotes an
T13717 31747-31754 NN denotes isotype
T13718 31755-31762 NN denotes control
T13719 31763-31771 NN denotes antibody
T13720 31771-31772 -COMMA- denotes ,
T13721 31773-31777 IN denotes with
T13722 31778-31780 CC denotes or
T13723 31781-31788 IN denotes without
T13724 31789-31792 CD denotes 500
T13725 31793-31797 NN denotes U/ml
T13726 31798-31803 NN denotes mouse
T13727 31804-31808 NN denotes IL-4
T13728 31809-31810 -LRB- denotes (
T13729 31810-31812 NN denotes BD
T13730 31813-31824 NNP denotes Biosciences
T13731 31824-31825 -RRB- denotes )
T13732 31827-31830 NN denotes RNA
T13733 31831-31834 VB denotes was
T13734 31835-31843 VB denotes isolated
T13735 31844-31849 VB denotes using
T13736 31850-31856 NN denotes Trizol
T13737 31857-31858 -LRB- denotes (
T13738 31858-31868 NN denotes Invitrogen
T13739 31868-31869 -RRB- denotes )
T13740 31869-31870 -COMMA- denotes ,
T13741 31871-31874 CC denotes and
T13742 31875-31894 JJ denotes reverse-transcribed
T13743 31895-31896 -LRB- denotes (
T13744 31896-31907 NN denotes Superscript
T13745 31908-31911 CD denotes III
T13746 31912-31915 NN denotes kit
T13747 31915-31916 -COMMA- denotes ,
T13748 31917-31927 NN denotes Invitrogen
T13749 31927-31928 -RRB- denotes )
T13750 31930-31942 JJ denotes Quantitative
T13751 31943-31946 NN denotes PCR
T13752 31947-31950 IN denotes for
T13753 31951-31954 NN denotes GLε
T13754 31955-31958 CC denotes and
T13755 31959-31964 NN denotes Hprt1
T13756 31965-31968 VB denotes was
T13757 31969-31978 VB denotes performed
T13758 31979-31984 VB denotes using
T13759 31985-31989 NN denotes SYBR
T13760 31990-31995 NN denotes GREEN
T13761 31996-32002 NN denotes master
T13762 32003-32006 NN denotes mix
T13763 32007-32008 -LRB- denotes (
T13764 32008-32015 NNP denotes Applied
T13765 32016-32026 NNP denotes Biosystems
T13766 32026-32027 -RRB- denotes )
T13767 32027-32028 -COMMA- denotes ,
T13768 32029-32032 CC denotes and
T13769 32033-32035 DT denotes an
T13770 32036-32043 NNP denotes Applied
T13771 32044-32054 NNP denotes Biosystems
T13772 32055-32061 NNP denotes 7900HT
T13773 32062-32066 NNP denotes Fast
T13774 32067-32076 NNP denotes Real-Time
T13775 32077-32080 NN denotes PCR
T13776 32081-32091 NN denotes instrument
T13777 32093-32103 NN denotes Expression
T13778 32104-32106 IN denotes of
T13779 32107-32110 NN denotes GLε
T13780 32111-32113 IN denotes in
T13781 32114-32118 DT denotes each
T13782 32119-32125 NN denotes sample
T13783 32126-32129 VB denotes was
T13784 32130-32140 VB denotes normalized
T13785 32141-32143 TO denotes to
T13786 32144-32147 DT denotes the
T13787 32148-32158 NN denotes expression
T13788 32159-32161 IN denotes of
T13789 32162-32167 NN denotes Hprt1
T14056 32170-32175 NN denotes NF-κB
T14057 32176-32179 CC denotes and
T14058 32180-32183 NN denotes JNK
T14059 32184-32194 NN denotes activation
T14060 32195-32201 NN denotes assays
T14061 32202-32205 IN denotes For
T14062 32206-32216 NN denotes activation
T14063 32217-32219 IN denotes of
T14064 32220-32224 NN denotes CD40
T14065 32225-32234 NN denotes signaling
T14066 32234-32235 -COMMA- denotes ,
T14067 32236-32241 CD denotes 5×104
T14068 32242-32245 NN denotes HI5
T14069 32246-32252 NN denotes insect
T14070 32253-32258 NN denotes cells
T14071 32259-32261 CC denotes or
T14072 32262-32265 NN denotes HI5
T14073 32266-32272 NN denotes insect
T14074 32273-32278 NN denotes cells
T14075 32279-32289 VB denotes expressing
T14076 32290-32295 NN denotes mouse
T14077 32296-32301 NN denotes CD154
T14078 32302-32303 -LRB- denotes (
T14079 32303-32307 NN denotes CD40
T14080 32308-32314 NN denotes ligand
T14081 32314-32315 -RRB- denotes )
T14082 32316-32320 VB denotes were
T14083 32321-32326 VB denotes added
T14084 32327-32329 TO denotes to
T14085 32330-32335 CD denotes 1×106
T14086 32336-32337 NN denotes B
T14087 32338-32343 NN denotes cells
T14088 32345-32350 NN denotes Cells
T14089 32351-32355 VB denotes were
T14090 32356-32367 VB denotes centrifuged
T14091 32368-32371 IN denotes for
T14092 32372-32373 CD denotes 1
T14093 32374-32380 NN denotes minute
T14094 32381-32383 IN denotes at
T14095 32384-32387 CD denotes 400
T14096 32388-32389 SYM denotes x
T14097 32390-32391 NN denotes g
T14098 32392-32393 -LRB- denotes (
T14099 32393-32395 TO denotes to
T14100 32396-32403 VB denotes promote
T14101 32404-32411 NN denotes contact
T14102 32412-32419 IN denotes between
T14103 32420-32426 NN denotes ligand
T14104 32427-32432 NN denotes cells
T14105 32433-32436 CC denotes and
T14106 32437-32438 NN denotes B
T14107 32439-32444 NN denotes cells
T14108 32444-32445 -RRB- denotes )
T14109 32446-32450 RB denotes then
T14110 32451-32460 VB denotes incubated
T14111 32461-32463 IN denotes at
T14112 32464-32468 NN denotes 37°C
T14113 32469-32472 IN denotes for
T14114 32473-32478 NN denotes times
T14115 32479-32488 VB denotes indicated
T14116 32489-32491 IN denotes in
T14117 32492-32496 NNP denotes Fig.
T14118 32497-32498 CD denotes 4
T14119 32500-32509 VB denotes Following
T14120 32510-32521 NN denotes stimulation
T14121 32521-32522 -COMMA- denotes ,
T14122 32523-32528 NN denotes cells
T14123 32529-32533 VB denotes were
T14124 32534-32541 VB denotes chilled
T14125 32542-32544 IN denotes on
T14126 32545-32548 NN denotes ice
T14127 32549-32552 IN denotes for
T14128 32553-32554 CD denotes 2
T14129 32555-32562 NN denotes minutes
T14130 32564-32568 NN denotes Cell
T14131 32569-32576 NN denotes pellets
T14132 32577-32581 VB denotes were
T14133 32582-32591 VB denotes dissolved
T14134 32592-32594 IN denotes in
T14135 32595-32597 NN denotes 2X
T14136 32598-32606 NN denotes SDS-PAGE
T14137 32607-32614 VB denotes loading
T14138 32615-32621 NN denotes buffer
T14139 32621-32622 -COMMA- denotes ,
T14140 32623-32632 VB denotes sonicated
T14141 32632-32633 -COMMA- denotes ,
T14142 32634-32637 CC denotes and
T14143 32638-32644 VB denotes heated
T14144 32645-32648 IN denotes for
T14145 32649-32650 CD denotes 5
T14146 32651-32658 NN denotes minutes
T14147 32659-32661 IN denotes at
T14148 32662-32667 NNP denotes 95°C.
T14149 32668-32673 NNP denotes Total
T14150 32674-32678 NN denotes cell
T14151 32679-32686 NN denotes lysates
T14152 32687-32688 -LRB- denotes (
T14153 32688-32693 CD denotes 1×105
T14154 32694-32698 NN denotes cell
T14155 32699-32710 NN denotes equivalents
T14156 32711-32714 IN denotes per
T14157 32715-32719 NN denotes lane
T14158 32719-32720 -RRB- denotes )
T14159 32721-32725 VB denotes were
T14160 32726-32738 VB denotes fractionated
T14161 32739-32741 IN denotes by
T14162 32742-32750 NN denotes SDS-PAGE
T14163 32751-32754 CC denotes and
T14164 32755-32762 VB denotes blotted
T14165 32763-32765 TO denotes to
T14166 32766-32780 NN denotes polyvinylidine
T14167 32781-32789 NN denotes fluoride
T14168 32790-32791 -LRB- denotes (
T14169 32791-32795 NN denotes PVDF
T14170 32795-32796 -RRB- denotes )
T14171 32797-32806 NN denotes membranes
T14172 32808-32817 NN denotes Membranes
T14173 32818-32822 VB denotes were
T14174 32823-32829 VB denotes probed
T14175 32830-32834 IN denotes with
T14176 32835-32845 NN denotes antibodies
T14177 32846-32848 IN denotes as
T14178 32849-32858 VB denotes indicated
T14179 32859-32861 IN denotes in
T14180 32862-32866 NNP denotes Fig.
T14181 32867-32868 CD denotes 4
T14182 32870-32886 JJ denotes Chemiluminescent
T14183 32887-32896 NN denotes detection
T14184 32897-32898 -LRB- denotes (
T14185 32898-32904 NNP denotes Pierce
T14186 32905-32918 NNP denotes Biotechnology
T14187 32918-32919 -RRB- denotes )
T14188 32920-32923 VB denotes was
T14189 32924-32928 VB denotes used
T14190 32929-32932 IN denotes for
T14191 32933-32936 DT denotes the
T14192 32937-32950 NN denotes visualization
T14193 32951-32953 IN denotes of
T14194 32954-32959 NN denotes bands
T14195 32960-32962 IN denotes on
T14196 32963-32970 JJ denotes Western
T14197 32971-32976 NN denotes blots
T14198 32978-32984 NN denotes Images
T14199 32985-32987 IN denotes of
T14200 32988-32993 NN denotes blots
T14201 32994-32998 VB denotes were
T14202 32999-33007 VB denotes recorded
T14203 33008-33012 IN denotes with
T14204 33013-33014 DT denotes a
T14205 33015-33024 JJ denotes low-light
T14206 33025-33032 NN denotes imaging
T14207 33033-33039 NN denotes system
T14208 33040-33041 -LRB- denotes (
T14209 33041-33048 NN denotes LAS4000
T14210 33048-33049 -COMMA- denotes ,
T14211 33050-33054 NNP denotes Fuji
T14212 33055-33062 NNP denotes Medical
T14213 33063-33070 NNP denotes Systems
T14214 33070-33071 -RRB- denotes )
T14215 33072-33075 CC denotes and
T14216 33076-33078 IN denotes on
T14217 33079-33084 NN denotes X-ray
T14218 33085-33089 NN denotes film
T14502 33092-33112 NN denotes Immunoprecipitations
T14503 33113-33132 NN denotes Immunoprecipitation
T14504 33133-33135 IN denotes of
T14505 33136-33140 NN denotes CD40
T14506 33141-33144 VB denotes was
T14507 33145-33154 VB denotes performed
T14508 33155-33157 IN denotes by
T14509 33158-33167 VB denotes activated
T14510 33168-33176 NN denotes receptor
T14511 33177-33184 NN denotes capture
T14512 33184-33185 -COMMA- denotes ,
T14513 33186-33188 IN denotes as
T14514 33189-33199 RB denotes previously
T14515 33200-33209 VB denotes described
T14516 33210-33211 -LRB- denotes [
T14517 33211-33212 CD denotes 6
T14518 33212-33213 -RRB- denotes ]
T14519 33215-33222 NNP denotes Briefly
T14520 33222-33223 -COMMA- denotes ,
T14521 33224-33229 CD denotes 3×107
T14522 33230-33235 NN denotes cells
T14523 33236-33237 -LRB- denotes (
T14524 33237-33243 NN denotes A20.2J
T14525 33244-33247 CC denotes and
T14526 33248-33259 NN denotes derivatives
T14527 33259-33260 -RRB- denotes )
T14528 33261-33265 VB denotes were
T14529 33266-33275 VB denotes incubated
T14530 33276-33279 IN denotes for
T14531 33280-33282 CD denotes 60
T14532 33283-33290 NN denotes minutes
T14533 33291-33293 IN denotes at
T14534 33294-33298 NN denotes room
T14535 33299-33310 NN denotes temperature
T14536 33311-33315 IN denotes with
T14537 33316-33318 CD denotes 10
T14538 33319-33321 NN denotes µl
T14539 33322-33330 JJ denotes magnetic
T14540 33331-33338 NN denotes protein
T14541 33339-33340 NN denotes G
T14542 33341-33346 NN denotes beads
T14543 33347-33348 -LRB- denotes (
T14544 33348-33353 JJ denotes Dynal
T14545 33353-33354 -RRB- denotes )
T14546 33355-33365 JJ denotes pre-coated
T14547 33366-33370 IN denotes with
T14548 33371-33373 CD denotes 10
T14549 33374-33376 NN denotes µg
T14550 33377-33381 NN denotes goat
T14551 33382-33390 JJ denotes anti-rat
T14552 33391-33394 NN denotes IgG
T14553 33395-33396 -LRB- denotes (
T14554 33396-33403 NN denotes Jackson
T14555 33403-33404 -RRB- denotes )
T14556 33404-33405 -COMMA- denotes ,
T14557 33406-33409 CC denotes and
T14558 33410-33412 CD denotes 10
T14559 33413-33415 NN denotes µg
T14560 33416-33425 NN denotes anti-CD40
T14561 33426-33427 -LRB- denotes (
T14562 33427-33431 NN denotes 1C10
T14563 33431-33432 -RRB- denotes )
T14564 33433-33435 CC denotes or
T14565 33436-33438 DT denotes an
T14566 33439-33446 NN denotes isotype
T14567 33447-33454 NN denotes control
T14568 33455-33463 NN denotes antibody
T14569 33464-33465 -LRB- denotes (
T14570 33465-33470 NN denotes mAb72
T14571 33470-33471 -RRB- denotes )
T14572 33473-33484 NN denotes Cells/beads
T14573 33485-33489 VB denotes were
T14574 33490-33494 RB denotes then
T14575 33495-33503 VB denotes pelleted
T14576 33504-33506 IN denotes by
T14577 33507-33521 NN denotes centrifugation
T14578 33522-33525 CC denotes and
T14579 33526-33531 VB denotes lysed
T14580 33532-33534 IN denotes in
T14581 33535-33541 NN denotes buffer
T14582 33542-33552 VB denotes containing
T14583 33553-33554 CD denotes 1
T14584 33554-33555 NN denotes %
T14585 33556-33562 NN denotes Triton
T14586 33563-33567 NN denotes X100
T14587 33569-33574 NN denotes Beads
T14588 33575-33579 VB denotes were
T14589 33580-33586 VB denotes washed
T14590 33587-33591 IN denotes with
T14591 33592-33597 NN denotes lysis
T14592 33598-33604 NN denotes buffer
T14593 33605-33607 TO denotes to
T14594 33608-33614 VB denotes remove
T14595 33615-33622 JJ denotes unbound
T14596 33623-33631 NN denotes material
T14597 33633-33635 IN denotes In
T14598 33636-33640 DT denotes some
T14599 33641-33652 NN denotes experiments
T14600 33652-33653 -COMMA- denotes ,
T14601 33654-33662 NN denotes material
T14602 33663-33673 VB denotes associated
T14603 33674-33678 IN denotes with
T14604 33679-33682 DT denotes the
T14605 33683-33688 NN denotes beads
T14606 33689-33692 VB denotes was
T14607 33693-33709 VB denotes dephosphorylated
T14608 33710-33714 IN denotes with
T14609 33715-33721 NN denotes lambda
T14610 33722-33733 NN denotes phosphatase
T14611 33734-33735 -LRB- denotes (
T14612 33735-33738 NNP denotes New
T14613 33739-33746 NNP denotes England
T14614 33747-33754 NNP denotes BioLabs
T14615 33754-33755 -RRB- denotes )
T14616 33756-33758 IN denotes as
T14617 33759-33762 IN denotes per
T14618 33763-33775 NN denotes manufacturer
T14619 33775-33777 POS denotes 's
T14620 33778-33790 NN denotes instructions
T14621 33792-33797 NN denotes Beads
T14622 33798-33802 VB denotes were
T14623 33803-33814 VB denotes resuspended
T14624 33815-33817 IN denotes in
T14625 33818-33820 NN denotes 2X
T14626 33821-33829 NN denotes SDS-PAGE
T14627 33830-33836 NN denotes sample
T14628 33837-33843 NN denotes buffer
T14629 33844-33847 CC denotes and
T14630 33848-33854 VB denotes heated
T14631 33855-33858 IN denotes for
T14632 33859-33860 CD denotes 5
T14633 33861-33868 NN denotes minutes
T14634 33869-33871 IN denotes at
T14635 33872-33877 NNP denotes 95°C.
T14636 33878-33886 NNP denotes Material
T14637 33887-33893 VB denotes eluted
T14638 33894-33898 IN denotes from
T14639 33899-33902 DT denotes the
T14640 33903-33908 NN denotes beads
T14641 33909-33912 VB denotes was
T14642 33913-33925 VB denotes fractionated
T14643 33926-33928 IN denotes by
T14644 33929-33937 NN denotes SDS-PAGE
T14645 33938-33941 CC denotes and
T14646 33942-33953 VB denotes transferred
T14647 33954-33956 TO denotes to
T14648 33957-33961 NN denotes PVDF
T14649 33962-33971 NN denotes membranes
T14650 33972-33975 IN denotes for
T14651 33976-33983 JJ denotes Western
T14652 33984-33992 NN denotes blotting
T14653 33994-33996 IN denotes In
T14654 33997-34001 DT denotes some
T14655 34002-34013 NN denotes experiments
T14656 34013-34014 -COMMA- denotes ,
T14657 34015-34020 NN denotes cells
T14658 34021-34025 VB denotes were
T14659 34026-34034 VB denotes cultured
T14660 34035-34038 IN denotes for
T14661 34039-34040 CD denotes 6
T14662 34041-34044 NN denotes hrs
T14663 34045-34049 IN denotes with
T14664 34050-34052 CD denotes 25
T14665 34053-34055 NN denotes µM
T14666 34056-34075 JJ denotes antennapedia-linked
T14667 34076-34083 NN denotes SMAC-N7
T14668 34084-34091 NN denotes peptide
T14669 34092-34093 -LRB- denotes (
T14670 34093-34103 NN denotes Calbiochem
T14671 34103-34104 -RRB- denotes )
T14672 34105-34107 CC denotes or
T14673 34108-34110 DT denotes an
T14674 34111-34122 JJ denotes appropriate
T14675 34123-34129 NN denotes volume
T14676 34130-34132 IN denotes of
T14677 34133-34136 DT denotes the
T14678 34137-34144 NN denotes solvent
T14679 34145-34149 VB denotes used
T14680 34150-34153 IN denotes for
T14681 34154-34157 DT denotes the
T14682 34158-34165 NN denotes peptide
T14683 34166-34167 -LRB- denotes (
T14684 34167-34171 NN denotes DMSO
T14685 34171-34172 -RRB- denotes )
T14686 34174-34179 IN denotes After
T14687 34180-34190 NN denotes incubation
T14688 34190-34191 -COMMA- denotes ,
T14689 34192-34197 NN denotes cells
T14690 34198-34199 -LRB- denotes (
T14691 34199-34201 IN denotes in
T14692 34202-34210 JJ denotes peptide-
T14693 34211-34213 CC denotes or
T14694 34214-34229 JJ denotes DMSO-containing
T14695 34230-34236 NN denotes medium
T14696 34236-34237 -RRB- denotes )
T14697 34238-34242 VB denotes were
T14698 34243-34253 VB denotes stimulated
T14699 34254-34258 IN denotes with
T14700 34259-34274 JJ denotes antibody-coated
T14701 34275-34280 NN denotes beads
T14702 34281-34283 IN denotes as
T14703 34284-34292 VB denotes outlined
T14704 34293-34298 IN denotes above
R10000 T12743 T12741 arg2Of sequence,to
R10001 T12743 T12742 arg1Of sequence,genomic
R10002 T12745 T12744 arg2Of approximate,;
R10003 T12746 T12739 arg2Of position,(
R10004 T12746 T12740 arg1Of position,homologous
R10005 T12746 T12745 arg1Of position,approximate
R10006 T12746 T12747 arg2Of position,noted
R10007 T12747 T12748 arg1Of noted,in
R10008 T12749 T12748 arg2Of Fig.,in
R10009 T12749 T12750 arg1Of Fig.,1
R10010 T12751 T12739 arg3Of ),(
R10011 T12752 T12737 arg2Of and,were
R10012 T12753 T12752 arg2Of 5′-caatccatcttgttcagccat-3′,and
R10013 T12753 T12754 arg1Of 5′-caatccatcttgttcagccat-3′,(
R10014 T12757 T12754 arg2Of sequence,(
R10015 T12757 T12755 arg1Of sequence,homologous
R10016 T12757 T12756 arg1Of sequence,to
R10017 T12757 T12758 arg1Of sequence,in
R10018 T12759 T12758 arg2Of NeoR,in
R10019 T12760 T12754 arg3Of ),(
R10020 T12761 T12762 arg2Of Clones,in
R10021 T12761 T12763 arg1Of Clones,which
R10022 T12761 T12771 arg1Of Clones,were
R10023 T12761 T12773 arg2Of Clones,transfected
R10024 T12761 T12784 arg1Of Clones,remove
R10025 T12765 T12764 arg1Of copy,one
R10026 T12765 T12766 arg1Of copy,of
R10027 T12765 T12768 arg1Of copy,had
R10028 T12765 T12769 arg1Of copy,been
R10029 T12765 T12770 arg2Of copy,disrupted
R10030 T12767 T12766 arg2Of Rnf31,of
R10031 T12770 T12762 arg1Of disrupted,in
R10032 T12770 T12768 arg2Of disrupted,had
R10033 T12770 T12769 arg2Of disrupted,been
R10034 T12773 T12771 arg2Of transfected,were
R10035 T12773 T12772 arg1Of transfected,transiently
R10036 T12773 T12774 arg1Of transfected,with
R10037 T12773 T12780 arg1Of transfected,","
R10038 T12773 T12781 arg1Of transfected,in
R10039 T12777 T12774 arg2Of plasmid,with
R10040 T12777 T12775 arg1Of plasmid,an
R10041 T12777 T12776 arg1Of plasmid,expression
R10042 T12777 T12778 arg1Of plasmid,encoding
R10043 T12779 T12778 arg2Of Cre,encoding
R10044 T12782 T12781 arg2Of order,in
R10045 T12784 T12781 arg3Of remove,in
R10046 T12784 T12783 arg1Of remove,to
R10047 T12785 T12784 arg2Of NeoR,remove
R10048 T12787 T12786 arg1Of subclones,G418-sensitive
R10049 T12787 T12788 arg1Of subclones,were
R10050 T12787 T12789 arg2Of subclones,subjected
R10051 T12789 T12788 arg2Of subjected,were
R10052 T12789 T12790 arg1Of subjected,to
R10053 T12789 T12796 modOf subjected,to
R10054 T12793 T12790 arg2Of round,to
R10055 T12793 T12791 arg1Of round,a
R10056 T12793 T12792 arg1Of round,second
R10057 T12793 T12794 arg1Of round,of
R10058 T12795 T12794 arg2Of targeting,of
R10059 T12797 T12796 arg1Of disrupt,to
R10060 T12800 T12797 arg2Of copy,disrupt
R10061 T12800 T12798 arg1Of copy,the
R10062 T12800 T12799 arg1Of copy,remaining
R10063 T12800 T12801 arg1Of copy,of
R10064 T12802 T12801 arg2Of Rnf31,of
R10065 T12804 T12803 arg1Of clones,G418-resistant
R10066 T12804 T12805 arg1Of clones,were
R10067 T12804 T12806 arg2Of clones,tested
R10068 T12806 T12805 arg2Of tested,were
R10069 T12806 T12807 arg1Of tested,for
R10070 T12809 T12807 arg2Of recombination,for
R10071 T12809 T12808 arg1Of recombination,homologous
R10072 T12811 T12812 arg1Of PCR,and
R10073 T12813 T12812 arg2Of HOIP,and
R10074 T12815 T12806 arg1Of expression,tested
R10075 T12815 T12810 arg2Of expression,by
R10076 T12815 T12811 arg1Of expression,PCR
R10077 T12815 T12813 arg1Of expression,HOIP
R10078 T12815 T12814 arg1Of expression,protein
R10079 T12815 T12816 arg1Of expression,by
R10080 T12818 T12816 arg2Of blot,by
R10081 T12818 T12817 arg1Of blot,Western
R10292 T13107 T13106 arg1Of transduction,Retroviral
R10293 T13110 T13108 arg1Of vector,A
R10294 T13110 T13109 arg1Of vector,retroviral
R10295 T13110 T13111 arg1Of vector,(
R10296 T13110 T13114 arg1Of vector,was
R10297 T13110 T13115 arg2Of vector,used
R10298 T13112 T13111 arg2Of pMIP,(
R10299 T13113 T13111 arg3Of ),(
R10300 T13115 T13114 arg2Of used,was
R10301 T13115 T13116 arg1Of used,for
R10302 T13115 T13124 arg1Of used,and
R10303 T13119 T13116 arg2Of transduction,for
R10304 T13119 T13117 arg1Of transduction,the
R10305 T13119 T13118 arg1Of transduction,stable
R10306 T13119 T13120 arg1Of transduction,of
R10307 T13119 T13122 arg1Of transduction,with
R10308 T13121 T13120 arg2Of cells,of
R10309 T13123 T13122 arg2Of HOIP,with
R10310 T13128 T13125 arg1Of pMIP,IKKγ
R10311 T13128 T13126 arg1Of pMIP,cDNA
R10312 T13128 T13127 arg1Of pMIP,constructs.
R10313 T13128 T13129 arg1Of pMIP,was
R10314 T13128 T13130 arg2Of pMIP,constructed
R10315 T13130 T13124 arg2Of constructed,and
R10316 T13130 T13129 arg2Of constructed,was
R10317 T13130 T13131 arg1Of constructed,by
R10318 T13132 T13131 arg2Of replacing,by
R10319 T13132 T13141 arg1Of replacing,[
R10320 T13132 T13144 arg1Of replacing,with
R10321 T13134 T13135 arg1Of IRES,and
R10322 T13135 T13132 arg2Of and,replacing
R10323 T13135 T13133 arg1Of and,the
R10324 T13135 T13137 arg1Of and,encoding
R10325 T13136 T13135 arg2Of GFP,and
R10326 T13137 T13139 arg1Of encoding,in
R10327 T13138 T13137 arg2Of sequences,encoding
R10328 T13140 T13139 arg2Of pMIG,in
R10329 T13142 T13141 arg2Of 31,[
R10330 T13143 T13141 arg3Of ],[
R10331 T13146 T13147 arg1Of IRES,and
R10332 T13148 T13147 arg2Of puromycin,and
R10333 T13150 T13144 arg2Of gene,with
R10334 T13150 T13145 arg1Of gene,the
R10335 T13150 T13146 arg1Of gene,IRES
R10336 T13150 T13148 arg1Of gene,puromycin
R10337 T13150 T13149 arg1Of gene,resistance
R10338 T13150 T13151 arg1Of gene,from
R10339 T13152 T13151 arg2Of pIRESpuro2,from
R10340 T13152 T13153 arg1Of pIRESpuro2,(
R10341 T13154 T13153 arg2Of Clontech,(
R10342 T13155 T13153 arg3Of ),(
R10343 T13159 T13156 arg1Of cDNA,FLAG-tagged
R10344 T13159 T13157 arg1Of cDNA,mouse
R10345 T13159 T13158 arg1Of cDNA,HOIP
R10346 T13159 T13160 arg1Of cDNA,was
R10347 T13159 T13161 arg2Of cDNA,inserted
R10348 T13161 T13160 arg2Of inserted,was
R10349 T13161 T13162 arg1Of inserted,into
R10350 T13166 T13162 arg2Of site,into
R10351 T13166 T13163 arg1Of site,the
R10352 T13166 T13164 arg1Of site,multiple
R10353 T13166 T13165 arg1Of site,cloning
R10354 T13166 T13167 arg1Of site,of
R10355 T13168 T13167 arg2Of pMIP,of
R10356 T13171 T13169 arg1Of construct,A
R10357 T13171 T13170 arg1Of construct,pMIP
R10358 T13171 T13172 arg1Of construct,encoding
R10359 T13171 T13186 arg1Of construct,was
R10360 T13171 T13188 arg2Of construct,prepared
R10361 T13172 T13175 arg1Of encoding,with
R10362 T13174 T13172 arg2Of IKKγ,encoding
R10363 T13174 T13173 arg1Of IKKγ,mouse
R10364 T13181 T13177 arg1Of peptide,amino
R10365 T13181 T13178 arg1Of peptide,terminal
R10366 T13181 T13179 arg1Of peptide,birch
R10367 T13181 T13180 arg1Of peptide,profilin
R10368 T13181 T13182 arg1Of peptide,(
R10369 T13183 T13182 arg2Of BP,(
R10370 T13184 T13182 arg3Of ),(
R10371 T13185 T13175 arg2Of -tag,with
R10372 T13185 T13176 arg1Of -tag,an
R10373 T13185 T13181 arg1Of -tag,peptide
R10374 T13188 T13186 arg2Of prepared,was
R10375 T13188 T13187 arg1Of prepared,also
R10376 T13190 T13189 arg1Of particles,Retroviral
R10377 T13190 T13191 arg1Of particles,were
R10378 T13190 T13192 arg2Of particles,generated
R10379 T13192 T13191 arg2Of generated,were
R10380 T13192 T13193 arg1Of generated,by
R10381 T13195 T13193 arg2Of transfection,by
R10382 T13195 T13194 arg1Of transfection,transient
R10383 T13195 T13196 arg1Of transfection,of
R10384 T13195 T13199 arg1Of transfection,with
R10385 T13198 T13196 arg2Of cells,of
R10386 T13198 T13197 arg1Of cells,293T
R10387 T13200 T13201 arg1Of pMIP,","
R10388 T13201 T13204 arg1Of ",",or
R10389 T13202 T13201 arg2Of pMIP-HOIP,","
R10390 T13204 T13203 arg1Of or,","
R10391 T13204 T13206 arg1Of or,and
R10392 T13205 T13204 arg2Of pMIP-IKKγ,or
R10393 T13206 T13199 arg2Of and,with
R10394 T13210 T13206 arg2Of pCL-Eco,and
R10395 T13210 T13207 arg1Of pCL-Eco,the
R10396 T13210 T13208 arg1Of pCL-Eco,packaging
R10397 T13210 T13209 arg1Of pCL-Eco,vector
R10398 T13210 T13211 arg1Of pCL-Eco,[
R10399 T13212 T13211 arg2Of 32,[
R10400 T13213 T13211 arg3Of ],[
R10401 T13215 T13214 arg1Of days,Two
R10402 T13216 T13215 arg1Of after,days
R10403 T13217 T13216 arg2Of transfection,after
R10404 T13220 T13219 arg1Of supernatants,culture
R10405 T13220 T13221 arg1Of supernatants,were
R10406 T13220 T13222 arg2Of supernatants,harvested
R10407 T13220 T13224 arg2Of supernatants,filtered
R10408 T13222 T13223 arg1Of harvested,and
R10409 T13223 T13216 arg1Of and,after
R10410 T13223 T13218 arg1Of and,","
R10411 T13223 T13221 arg2Of and,were
R10412 T13224 T13223 arg2Of filtered,and
R10413 T13225 T13226 arg1Of A20.2J,or
R10414 T13227 T13226 arg2Of HOIP-deficient,or
R10415 T13228 T13225 arg1Of cells,A20.2J
R10416 T13228 T13227 arg1Of cells,HOIP-deficient
R10417 T13228 T13229 arg1Of cells,were
R10418 T13228 T13230 arg2Of cells,added
R10419 T13230 T13229 arg2Of added,were
R10420 T13230 T13231 arg1Of added,to
R10421 T13230 T13238 arg1Of added,with
R10422 T13233 T13231 arg2Of plates,to
R10423 T13233 T13232 arg1Of plates,24-well
R10424 T13233 T13234 arg1Of plates,(
R10425 T13236 T13234 arg2Of cells/well,(
R10426 T13236 T13235 arg1Of cells/well,2.5×105
R10427 T13237 T13234 arg3Of ),(
R10428 T13242 T13239 arg1Of supernatant,1
R10429 T13242 T13240 arg1Of supernatant,ml
R10430 T13242 T13241 arg1Of supernatant,virus-containing
R10431 T13242 T13243 arg1Of supernatant,and
R10432 T13243 T13238 arg2Of and,with
R10433 T13246 T13243 arg2Of polybrene,and
R10434 T13246 T13244 arg1Of polybrene,8
R10435 T13246 T13245 arg1Of polybrene,µg/ml
R10436 T13247 T13248 arg1Of Plates,were
R10437 T13247 T13249 arg2Of Plates,centrifuged
R10438 T13249 T13248 arg2Of centrifuged,were
R10439 T13249 T13250 arg1Of centrifuged,at
R10440 T13249 T13254 arg1Of centrifuged,for
R10441 T13253 T13250 arg2Of g,at
R10442 T13253 T13251 arg1Of g,500
R10443 T13253 T13252 arg1Of g,x
R10444 T13256 T13254 arg2Of hrs,for
R10445 T13256 T13255 arg1Of hrs,2
R10446 T13256 T13257 arg1Of hrs,at
R10447 T13259 T13257 arg2Of temperature,at
R10448 T13259 T13258 arg1Of temperature,room
R10449 T13260 T13261 arg1Of Cells,were
R10450 T13260 T13262 arg2Of Cells,cultured
R10451 T13262 T13261 arg2Of cultured,were
R10452 T13262 T13263 arg1Of cultured,in
R10453 T13262 T13268 arg1Of cultured,for
R10454 T13267 T13263 arg2Of medium,in
R10455 T13267 T13264 arg1Of medium,B
R10456 T13267 T13265 arg1Of medium,cell
R10457 T13267 T13266 arg1Of medium,culture
R10458 T13270 T13268 arg2Of hrs,for
R10459 T13270 T13269 arg1Of hrs,48
R10460 T13270 T13271 arg1Of hrs,","
R10461 T13270 T13272 arg2Of hrs,after
R10462 T13270 T13273 arg1Of hrs,which
R10463 T13276 T13274 arg1Of cells,the
R10464 T13276 T13275 arg2Of cells,transduced
R10465 T13276 T13277 arg1Of cells,were
R10466 T13276 T13278 arg2Of cells,selected
R10467 T13278 T13272 arg1Of selected,after
R10468 T13278 T13277 arg2Of selected,were
R10469 T13278 T13279 arg1Of selected,in
R10470 T13282 T13279 arg2Of puromycin,in
R10471 T13282 T13280 arg1Of puromycin,3
R10472 T13282 T13281 arg1Of puromycin,µg/ml
R10560 T13428 T13427 arg1Of upregulation,CD80
R10561 T13428 T13429 arg1Of upregulation,and
R10562 T13431 T13429 arg2Of cytometry,and
R10563 T13431 T13430 arg1Of cytometry,flow
R10564 T13432 T13433 arg1Of Cells,(
R10565 T13432 T13439 arg1Of Cells,were
R10566 T13432 T13440 arg2Of Cells,cultured
R10567 T13434 T13433 arg2Of 5×104,(
R10568 T13434 T13435 arg1Of 5×104,in
R10569 T13437 T13435 arg2Of ml,in
R10570 T13437 T13436 arg1Of ml,2
R10571 T13438 T13433 arg3Of ),(
R10572 T13440 T13439 arg2Of cultured,were
R10573 T13440 T13441 arg1Of cultured,in
R10574 T13443 T13441 arg2Of plates,in
R10575 T13443 T13442 arg1Of plates,24-well
R10576 T13443 T13444 arg1Of plates,for
R10577 T13446 T13444 arg2Of days,for
R10578 T13446 T13445 arg1Of days,3
R10579 T13446 T13447 arg1Of days,with
R10580 T13450 T13448 arg1Of anti-CD40,5
R10581 T13450 T13449 arg1Of anti-CD40,µg/ml
R10582 T13450 T13451 arg1Of anti-CD40,or
R10583 T13451 T13447 arg2Of or,with
R10584 T13455 T13451 arg2Of antibody,or
R10585 T13455 T13452 arg1Of antibody,an
R10586 T13455 T13453 arg1Of antibody,isotype
R10587 T13455 T13454 arg1Of antibody,control
R10588 T13456 T13457 arg1Of Cells,were
R10589 T13456 T13458 arg2Of Cells,stained
R10590 T13458 T13457 arg2Of stained,were
R10591 T13458 T13459 arg1Of stained,for
R10592 T13461 T13460 arg1Of cytometry,flow
R10593 T13461 T13462 arg1Of cytometry,with
R10594 T13461 T13464 arg1Of cytometry,or
R10595 T13463 T13462 arg2Of FITC-anti-CD80,with
R10596 T13464 T13459 arg2Of or,for
R10597 T13468 T13464 arg2Of antibody,or
R10598 T13468 T13465 arg1Of antibody,an
R10599 T13468 T13466 arg1Of antibody,appropriate
R10600 T13468 T13467 arg1Of antibody,control
R10601 T13468 T13470 arg2Of antibody,described
R10602 T13470 T13469 arg1Of described,as
R10603 T13470 T13471 arg1Of described,[
R10604 T13472 T13471 arg2Of 28,[
R10605 T13473 T13471 arg3Of ],[
R10606 T13474 T13475 arg2Of Data,acquired
R10607 T13474 T13485 arg1Of Data,were
R10608 T13474 T13486 arg2Of Data,analyzed
R10609 T13475 T13476 arg1Of acquired,with
R10610 T13480 T13476 arg2Of cytometer,with
R10611 T13480 T13477 arg1Of cytometer,a
R10612 T13480 T13478 arg1Of cytometer,FACScan
R10613 T13480 T13479 arg1Of cytometer,flow
R10614 T13480 T13481 arg1Of cytometer,(
R10615 T13483 T13481 arg2Of Biosciences,(
R10616 T13483 T13482 arg1Of Biosciences,BD
R10617 T13484 T13481 arg3Of ),(
R10618 T13486 T13485 arg2Of analyzed,were
R10619 T13486 T13487 arg1Of analyzed,with
R10620 T13488 T13487 arg2Of WinMDI,with
R10621 T13488 T13489 arg1Of WinMDI,2.8
R10622 T13488 T13490 arg1Of WinMDI,(
R10623 T13493 T13490 arg2Of Institute,(
R10624 T13493 T13491 arg1Of Institute,Scripps
R10625 T13493 T13492 arg1Of Institute,Research
R10626 T13493 T13494 arg1Of Institute,","
R10627 T13496 T13494 arg2Of Diego,","
R10628 T13496 T13495 arg1Of Diego,San
R10629 T13496 T13497 arg1Of Diego,","
R10630 T13498 T13497 arg2Of CA,","
R10631 T13499 T13490 arg3Of ),(
R10768 T13686 T13684 arg1Of assay,GLε
R10769 T13686 T13685 arg1Of assay,transcript
R10770 T13689 T13687 arg1Of activation,The
R10771 T13689 T13688 arg1Of activation,CD40-simulated
R10772 T13689 T13690 arg1Of activation,of
R10773 T13689 T13693 arg1Of activation,was
R10774 T13689 T13694 arg2Of activation,evaluated
R10775 T13692 T13690 arg2Of transcription,of
R10776 T13692 T13691 arg1Of transcription,GLε
R10777 T13694 T13693 arg2Of evaluated,was
R10778 T13694 T13695 arg1Of evaluated,as
R10779 T13697 T13696 arg1Of reported,previously
R10780 T13699 T13695 arg2Of 33,as
R10781 T13699 T13697 arg1Of 33,reported
R10782 T13699 T13698 arg2Of 33,[
R10783 T13700 T13698 arg3Of ],[
R10784 T13704 T13703 arg1Of cells,1×106
R10785 T13704 T13705 arg1Of cells,were
R10786 T13704 T13706 arg2Of cells,stimulated
R10787 T13706 T13701 arg1Of stimulated,Briefly
R10788 T13706 T13702 arg1Of stimulated,","
R10789 T13706 T13705 arg2Of stimulated,were
R10790 T13706 T13707 arg1Of stimulated,overnight
R10791 T13706 T13708 arg1Of stimulated,with
R10792 T13706 T13720 arg1Of stimulated,","
R10793 T13706 T13721 arg1Of stimulated,with
R10794 T13706 T13723 arg1Of stimulated,without
R10795 T13710 T13709 arg1Of antibody,anti-CD40
R10796 T13710 T13711 arg1Of antibody,(
R10797 T13710 T13715 arg1Of antibody,or
R10798 T13713 T13711 arg2Of µg/ml,(
R10799 T13713 T13712 arg1Of µg/ml,10
R10800 T13714 T13711 arg3Of ),(
R10801 T13715 T13708 arg2Of or,with
R10802 T13719 T13715 arg2Of antibody,or
R10803 T13719 T13716 arg1Of antibody,an
R10804 T13719 T13717 arg1Of antibody,isotype
R10805 T13719 T13718 arg1Of antibody,control
R10806 T13721 T13722 arg1Of with,or
R10807 T13723 T13722 arg2Of without,or
R10808 T13727 T13721 arg2Of IL-4,with
R10809 T13727 T13723 arg2Of IL-4,without
R10810 T13727 T13724 arg1Of IL-4,500
R10811 T13727 T13725 arg1Of IL-4,U/ml
R10812 T13727 T13726 arg1Of IL-4,mouse
R10813 T13727 T13728 arg1Of IL-4,(
R10814 T13730 T13728 arg2Of Biosciences,(
R10815 T13730 T13729 arg1Of Biosciences,BD
R10816 T13731 T13728 arg3Of ),(
R10817 T13732 T13733 arg1Of RNA,was
R10818 T13732 T13734 arg2Of RNA,isolated
R10819 T13734 T13733 arg2Of isolated,was
R10820 T13735 T13734 arg3Of using,isolated
R10821 T13736 T13737 arg1Of Trizol,(
R10822 T13736 T13741 arg1Of Trizol,and
R10823 T13738 T13737 arg2Of Invitrogen,(
R10824 T13739 T13737 arg3Of ),(
R10825 T13741 T13735 arg2Of and,using
R10826 T13741 T13740 arg1Of and,","
R10827 T13742 T13741 arg2Of reverse-transcribed,and
R10828 T13742 T13743 arg1Of reverse-transcribed,(
R10829 T13746 T13743 arg2Of kit,(
R10830 T13746 T13744 arg1Of kit,Superscript
R10831 T13746 T13745 arg1Of kit,III
R10832 T13746 T13747 arg1Of kit,","
R10833 T13748 T13747 arg2Of Invitrogen,","
R10834 T13749 T13743 arg3Of ),(
R10835 T13751 T13750 arg1Of PCR,Quantitative
R10836 T13751 T13752 arg1Of PCR,for
R10837 T13751 T13756 arg1Of PCR,was
R10838 T13751 T13757 arg2Of PCR,performed
R10839 T13753 T13754 arg1Of GLε,and
R10840 T13754 T13752 arg2Of and,for
R10841 T13755 T13754 arg2Of Hprt1,and
R10842 T13757 T13756 arg2Of performed,was
R10843 T13758 T13757 arg3Of using,performed
R10844 T13762 T13759 arg1Of mix,SYBR
R10845 T13762 T13760 arg1Of mix,GREEN
R10846 T13762 T13761 arg1Of mix,master
R10847 T13762 T13763 arg1Of mix,(
R10848 T13762 T13768 arg1Of mix,and
R10849 T13765 T13763 arg2Of Biosystems,(
R10850 T13765 T13764 arg1Of Biosystems,Applied
R10851 T13766 T13763 arg3Of ),(
R10852 T13768 T13758 arg2Of and,using
R10853 T13768 T13767 arg1Of and,","
R10854 T13774 T13770 arg1Of Real-Time,Applied
R10855 T13774 T13771 arg1Of Real-Time,Biosystems
R10856 T13774 T13772 arg1Of Real-Time,7900HT
R10857 T13774 T13773 arg1Of Real-Time,Fast
R10858 T13776 T13768 arg2Of instrument,and
R10859 T13776 T13769 arg1Of instrument,an
R10860 T13776 T13774 arg1Of instrument,Real-Time
R10861 T13776 T13775 arg1Of instrument,PCR
R10862 T13777 T13778 arg1Of Expression,of
R10863 T13777 T13780 arg1Of Expression,in
R10864 T13777 T13783 arg1Of Expression,was
R10865 T13777 T13784 arg2Of Expression,normalized
R10866 T13779 T13778 arg2Of GLε,of
R10867 T13782 T13780 arg2Of sample,in
R10868 T13782 T13781 arg1Of sample,each
R10869 T13784 T13783 arg2Of normalized,was
R10870 T13784 T13785 arg1Of normalized,to
R10871 T13787 T13785 arg2Of expression,to
R10872 T13787 T13786 arg1Of expression,the
R10873 T13787 T13788 arg1Of expression,of
R10874 T13789 T13788 arg2Of Hprt1,of
R11080 T14056 T14057 arg1Of NF-κB,and
R11081 T14058 T14057 arg2Of JNK,and
R11082 T14060 T14056 arg1Of assays,NF-κB
R11083 T14060 T14058 arg1Of assays,JNK
R11084 T14060 T14059 arg1Of assays,activation
R11085 T14062 T14061 arg2Of activation,For
R11086 T14062 T14063 arg1Of activation,of
R11087 T14065 T14063 arg2Of signaling,of
R11088 T14065 T14064 arg1Of signaling,CD40
R11089 T14070 T14067 arg1Of cells,5×104
R11090 T14070 T14068 arg1Of cells,HI5
R11091 T14070 T14069 arg1Of cells,insect
R11092 T14070 T14071 arg1Of cells,or
R11093 T14071 T14075 arg1Of or,expressing
R11094 T14071 T14082 arg1Of or,were
R11095 T14071 T14083 arg2Of or,added
R11096 T14074 T14071 arg2Of cells,or
R11097 T14074 T14072 arg1Of cells,HI5
R11098 T14074 T14073 arg1Of cells,insect
R11099 T14077 T14075 arg2Of CD154,expressing
R11100 T14077 T14076 arg1Of CD154,mouse
R11101 T14077 T14078 arg1Of CD154,(
R11102 T14080 T14078 arg2Of ligand,(
R11103 T14080 T14079 arg1Of ligand,CD40
R11104 T14081 T14078 arg3Of ),(
R11105 T14083 T14061 arg1Of added,For
R11106 T14083 T14066 arg1Of added,","
R11107 T14083 T14082 arg2Of added,were
R11108 T14083 T14084 arg1Of added,to
R11109 T14087 T14084 arg2Of cells,to
R11110 T14087 T14085 arg1Of cells,1×106
R11111 T14087 T14086 arg1Of cells,B
R11112 T14088 T14089 arg1Of Cells,were
R11113 T14088 T14090 arg2Of Cells,centrifuged
R11114 T14090 T14089 arg2Of centrifuged,were
R11115 T14090 T14091 arg1Of centrifuged,for
R11116 T14090 T14094 arg1Of centrifuged,at
R11117 T14090 T14099 modOf centrifuged,to
R11118 T14093 T14091 arg2Of minute,for
R11119 T14093 T14092 arg1Of minute,1
R11120 T14097 T14094 arg2Of g,at
R11121 T14097 T14095 arg1Of g,400
R11122 T14097 T14096 arg1Of g,x
R11123 T14097 T14098 arg1Of g,(
R11124 T14100 T14099 arg1Of promote,to
R11125 T14101 T14100 arg2Of contact,promote
R11126 T14101 T14102 arg1Of contact,between
R11127 T14104 T14103 arg1Of cells,ligand
R11128 T14104 T14105 arg1Of cells,and
R11129 T14105 T14102 arg2Of and,between
R11130 T14105 T14108 arg1Of and,)
R11131 T14105 T14110 arg2Of and,incubated
R11132 T14107 T14105 arg2Of cells,and
R11133 T14107 T14106 arg1Of cells,B
R11134 T14110 T14109 arg1Of incubated,then
R11135 T14110 T14111 arg1Of incubated,at
R11136 T14112 T14111 arg2Of 37°C,at
R11137 T14112 T14113 arg1Of 37°C,for
R11138 T14114 T14113 arg2Of times,for
R11139 T14114 T14115 arg2Of times,indicated
R11140 T14115 T14116 arg1Of indicated,in
R11141 T14117 T14116 arg2Of Fig.,in
R11142 T14117 T14118 arg1Of Fig.,4
R11143 T14120 T14119 arg2Of stimulation,Following
R11144 T14122 T14123 arg1Of cells,were
R11145 T14122 T14124 arg2Of cells,chilled
R11146 T14124 T14119 arg1Of chilled,Following
R11147 T14124 T14121 arg1Of chilled,","
R11148 T14124 T14123 arg2Of chilled,were
R11149 T14124 T14125 arg1Of chilled,on
R11150 T14124 T14127 arg1Of chilled,for
R11151 T14126 T14125 arg2Of ice,on
R11152 T14129 T14127 arg2Of minutes,for
R11153 T14129 T14128 arg1Of minutes,2
R11154 T14131 T14130 arg1Of pellets,Cell
R11155 T14131 T14132 arg1Of pellets,were
R11156 T14131 T14133 arg2Of pellets,dissolved
R11157 T14131 T14140 arg2Of pellets,sonicated
R11158 T14131 T14143 arg2Of pellets,heated
R11159 T14133 T14134 arg1Of dissolved,in
R11160 T14133 T14139 arg1Of dissolved,","
R11161 T14136 T14134 arg2Of SDS-PAGE,in
R11162 T14136 T14135 arg1Of SDS-PAGE,2X
R11163 T14136 T14137 arg1Of SDS-PAGE,loading
R11164 T14138 T14137 arg2Of buffer,loading
R11165 T14139 T14142 arg1Of ",",and
R11166 T14140 T14139 arg2Of sonicated,","
R11167 T14142 T14132 arg2Of and,were
R11168 T14142 T14141 arg1Of and,","
R11169 T14143 T14142 arg2Of heated,and
R11170 T14143 T14144 arg1Of heated,for
R11171 T14146 T14144 arg2Of minutes,for
R11172 T14146 T14145 arg1Of minutes,5
R11173 T14146 T14147 arg1Of minutes,at
R11174 T14149 T14148 arg1Of Total,95°C.
R11175 T14149 T14159 modOf Total,were
R11176 T14151 T14150 arg1Of lysates,cell
R11177 T14151 T14152 arg1Of lysates,(
R11178 T14151 T14159 arg1Of lysates,were
R11179 T14151 T14160 arg2Of lysates,fractionated
R11180 T14151 T14164 arg2Of lysates,blotted
R11181 T14155 T14152 arg2Of equivalents,(
R11182 T14155 T14153 arg1Of equivalents,1×105
R11183 T14155 T14154 arg1Of equivalents,cell
R11184 T14155 T14156 arg1Of equivalents,per
R11185 T14157 T14156 arg2Of lane,per
R11186 T14158 T14152 arg3Of ),(
R11187 T14160 T14163 arg1Of fractionated,and
R11188 T14162 T14160 arg1Of SDS-PAGE,fractionated
R11189 T14162 T14161 arg2Of SDS-PAGE,by
R11190 T14163 T14159 arg2Of and,were
R11191 T14164 T14163 arg2Of blotted,and
R11192 T14164 T14165 arg1Of blotted,to
R11193 T14167 T14165 arg2Of fluoride,to
R11194 T14167 T14166 arg1Of fluoride,polyvinylidine
R11195 T14167 T14168 arg1Of fluoride,(
R11196 T14169 T14168 arg2Of PVDF,(
R11197 T14170 T14168 arg3Of ),(
R11198 T14171 T14147 arg2Of membranes,at
R11199 T14171 T14149 arg1Of membranes,Total
R11200 T14172 T14173 arg1Of Membranes,were
R11201 T14172 T14174 arg2Of Membranes,probed
R11202 T14174 T14173 arg2Of probed,were
R11203 T14174 T14175 arg1Of probed,with
R11204 T14174 T14177 arg1Of probed,as
R11205 T14176 T14175 arg2Of antibodies,with
R11206 T14178 T14177 arg2Of indicated,as
R11207 T14178 T14179 arg1Of indicated,in
R11208 T14180 T14179 arg2Of Fig.,in
R11209 T14180 T14181 arg1Of Fig.,4
R11210 T14183 T14182 arg1Of detection,Chemiluminescent
R11211 T14183 T14184 arg1Of detection,(
R11212 T14183 T14188 arg1Of detection,was
R11213 T14183 T14189 arg2Of detection,used
R11214 T14186 T14184 arg2Of Biotechnology,(
R11215 T14186 T14185 arg1Of Biotechnology,Pierce
R11216 T14187 T14184 arg3Of ),(
R11217 T14189 T14188 arg2Of used,was
R11218 T14189 T14190 arg1Of used,for
R11219 T14192 T14190 arg2Of visualization,for
R11220 T14192 T14191 arg1Of visualization,the
R11221 T14192 T14193 arg1Of visualization,of
R11222 T14192 T14195 arg1Of visualization,on
R11223 T14194 T14193 arg2Of bands,of
R11224 T14197 T14195 arg2Of blots,on
R11225 T14197 T14196 arg1Of blots,Western
R11226 T14198 T14199 arg1Of Images,of
R11227 T14198 T14201 arg1Of Images,were
R11228 T14198 T14202 arg2Of Images,recorded
R11229 T14200 T14199 arg2Of blots,of
R11230 T14202 T14201 arg2Of recorded,were
R11231 T14202 T14203 arg1Of recorded,with
R11232 T14202 T14216 arg1Of recorded,on
R11233 T14203 T14215 arg1Of with,and
R11234 T14207 T14203 arg2Of system,with
R11235 T14207 T14204 arg1Of system,a
R11236 T14207 T14205 arg1Of system,low-light
R11237 T14207 T14206 arg1Of system,imaging
R11238 T14207 T14208 arg1Of system,(
R11239 T14209 T14208 arg2Of LAS4000,(
R11240 T14209 T14210 arg1Of LAS4000,","
R11241 T14213 T14210 arg2Of Systems,","
R11242 T14213 T14211 arg1Of Systems,Fuji
R11243 T14213 T14212 arg1Of Systems,Medical
R11244 T14214 T14208 arg3Of ),(
R11245 T14216 T14215 arg2Of on,and
R11246 T14218 T14216 arg2Of film,on
R11247 T14218 T14217 arg1Of film,X-ray
R11475 T14503 T14504 arg1Of Immunoprecipitation,of
R11476 T14503 T14506 arg1Of Immunoprecipitation,was
R11477 T14503 T14507 arg2Of Immunoprecipitation,performed
R11478 T14505 T14504 arg2Of CD40,of
R11479 T14507 T14506 arg2Of performed,was
R11480 T14511 T14507 arg1Of capture,performed
R11481 T14511 T14508 arg2Of capture,by
R11482 T14511 T14509 arg2Of capture,activated
R11483 T14511 T14510 arg1Of capture,receptor
R11484 T14511 T14512 arg1Of capture,","
R11485 T14511 T14513 arg1Of capture,as
R11486 T14515 T14513 arg2Of described,as
R11487 T14515 T14514 arg1Of described,previously
R11488 T14515 T14516 arg1Of described,[
R11489 T14517 T14516 arg2Of 6,[
R11490 T14518 T14516 arg3Of ],[
R11491 T14519 T14520 arg1Of Briefly,","
R11492 T14519 T14528 arg1Of Briefly,were
R11493 T14519 T14529 arg2Of Briefly,incubated
R11494 T14522 T14520 arg2Of cells,","
R11495 T14522 T14521 arg1Of cells,3×107
R11496 T14522 T14523 arg1Of cells,(
R11497 T14524 T14525 arg1Of A20.2J,and
R11498 T14525 T14523 arg2Of and,(
R11499 T14526 T14525 arg2Of derivatives,and
R11500 T14527 T14523 arg3Of ),(
R11501 T14529 T14528 arg2Of incubated,were
R11502 T14529 T14530 arg1Of incubated,for
R11503 T14532 T14531 arg1Of minutes,60
R11504 T14532 T14533 arg1Of minutes,at
R11505 T14532 T14536 arg1Of minutes,with
R11506 T14532 T14564 arg1Of minutes,or
R11507 T14535 T14533 arg2Of temperature,at
R11508 T14535 T14534 arg1Of temperature,room
R11509 T14542 T14536 arg2Of beads,with
R11510 T14542 T14537 arg1Of beads,10
R11511 T14542 T14538 arg1Of beads,µl
R11512 T14542 T14539 arg1Of beads,magnetic
R11513 T14542 T14540 arg1Of beads,protein
R11514 T14542 T14541 arg1Of beads,G
R11515 T14542 T14543 arg1Of beads,(
R11516 T14542 T14546 arg1Of beads,pre-coated
R11517 T14544 T14543 arg2Of Dynal,(
R11518 T14545 T14543 arg3Of ),(
R11519 T14546 T14547 arg1Of pre-coated,with
R11520 T14552 T14548 arg1Of IgG,10
R11521 T14552 T14549 arg1Of IgG,µg
R11522 T14552 T14550 arg1Of IgG,goat
R11523 T14552 T14551 arg1Of IgG,anti-rat
R11524 T14552 T14553 arg1Of IgG,(
R11525 T14552 T14557 arg1Of IgG,and
R11526 T14554 T14553 arg2Of Jackson,(
R11527 T14555 T14553 arg3Of ),(
R11528 T14557 T14547 arg2Of and,with
R11529 T14557 T14556 arg1Of and,","
R11530 T14560 T14557 arg2Of anti-CD40,and
R11531 T14560 T14558 arg1Of anti-CD40,10
R11532 T14560 T14559 arg1Of anti-CD40,µg
R11533 T14560 T14561 arg1Of anti-CD40,(
R11534 T14562 T14561 arg2Of 1C10,(
R11535 T14563 T14561 arg3Of ),(
R11536 T14564 T14530 arg2Of or,for
R11537 T14568 T14564 arg2Of antibody,or
R11538 T14568 T14565 arg1Of antibody,an
R11539 T14568 T14566 arg1Of antibody,isotype
R11540 T14568 T14567 arg1Of antibody,control
R11541 T14568 T14569 arg1Of antibody,(
R11542 T14570 T14569 arg2Of mAb72,(
R11543 T14571 T14569 arg3Of ),(
R11544 T14572 T14573 arg1Of Cells/beads,were
R11545 T14572 T14575 arg2Of Cells/beads,pelleted
R11546 T14572 T14579 arg2Of Cells/beads,lysed
R11547 T14575 T14578 arg1Of pelleted,and
R11548 T14577 T14575 arg1Of centrifugation,pelleted
R11549 T14577 T14576 arg2Of centrifugation,by
R11550 T14578 T14573 arg2Of and,were
R11551 T14578 T14574 arg1Of and,then
R11552 T14579 T14578 arg2Of lysed,and
R11553 T14579 T14580 arg1Of lysed,in
R11554 T14581 T14580 arg2Of buffer,in
R11555 T14581 T14582 arg1Of buffer,containing
R11556 T14583 T14584 arg1Of 1,%
R11557 T14586 T14582 arg2Of X100,containing
R11558 T14586 T14583 arg1Of X100,1
R11559 T14586 T14585 arg1Of X100,Triton
R11560 T14587 T14588 arg1Of Beads,were
R11561 T14587 T14589 arg2Of Beads,washed
R11562 T14589 T14588 arg2Of washed,were
R11563 T14589 T14590 arg1Of washed,with
R11564 T14589 T14593 modOf washed,to
R11565 T14592 T14590 arg2Of buffer,with
R11566 T14592 T14591 arg1Of buffer,lysis
R11567 T14594 T14593 arg1Of remove,to
R11568 T14596 T14594 arg2Of material,remove
R11569 T14596 T14595 arg1Of material,unbound
R11570 T14599 T14597 arg2Of experiments,In
R11571 T14599 T14598 arg1Of experiments,some
R11572 T14601 T14602 arg2Of material,associated
R11573 T14601 T14606 arg1Of material,was
R11574 T14601 T14607 arg2Of material,dephosphorylated
R11575 T14602 T14603 arg1Of associated,with
R11576 T14605 T14603 arg2Of beads,with
R11577 T14605 T14604 arg1Of beads,the
R11578 T14607 T14597 arg1Of dephosphorylated,In
R11579 T14607 T14600 arg1Of dephosphorylated,","
R11580 T14607 T14606 arg2Of dephosphorylated,was
R11581 T14607 T14608 arg1Of dephosphorylated,with
R11582 T14610 T14608 arg2Of phosphatase,with
R11583 T14610 T14609 arg1Of phosphatase,lambda
R11584 T14610 T14611 arg1Of phosphatase,(
R11585 T14610 T14617 arg1Of phosphatase,per
R11586 T14614 T14611 arg2Of BioLabs,(
R11587 T14614 T14612 arg1Of BioLabs,New
R11588 T14614 T14613 arg1Of BioLabs,England
R11589 T14615 T14611 arg3Of ),(
R11590 T14617 T14616 arg1Of per,as
R11591 T14618 T14619 arg2Of manufacturer,'s
R11592 T14620 T14617 arg2Of instructions,per
R11593 T14620 T14619 arg1Of instructions,'s
R11594 T14621 T14622 arg1Of Beads,were
R11595 T14621 T14623 arg2Of Beads,resuspended
R11596 T14621 T14630 arg2Of Beads,heated
R11597 T14623 T14624 arg1Of resuspended,in
R11598 T14623 T14629 arg1Of resuspended,and
R11599 T14628 T14624 arg2Of buffer,in
R11600 T14628 T14625 arg1Of buffer,2X
R11601 T14628 T14626 arg1Of buffer,SDS-PAGE
R11602 T14628 T14627 arg1Of buffer,sample
R11603 T14629 T14622 arg2Of and,were
R11604 T14630 T14629 arg2Of heated,and
R11605 T14630 T14631 arg1Of heated,for
R11606 T14633 T14632 arg1Of minutes,5
R11607 T14633 T14634 arg1Of minutes,at
R11608 T14633 T14641 arg1Of minutes,was
R11609 T14633 T14642 arg2Of minutes,fractionated
R11610 T14633 T14646 arg2Of minutes,transferred
R11611 T14636 T14634 arg2Of Material,at
R11612 T14636 T14635 arg1Of Material,95°C.
R11613 T14636 T14637 arg2Of Material,eluted
R11614 T14637 T14638 arg1Of eluted,from
R11615 T14640 T14638 arg2Of beads,from
R11616 T14640 T14639 arg1Of beads,the
R11617 T14642 T14645 arg1Of fractionated,and
R11618 T14644 T14642 arg1Of SDS-PAGE,fractionated
R11619 T14644 T14643 arg2Of SDS-PAGE,by
R11620 T14645 T14622 modOf and,were
R11621 T14645 T14641 arg2Of and,was
R11622 T14646 T14645 arg2Of transferred,and
R11623 T14646 T14647 arg1Of transferred,to
R11624 T14646 T14650 arg1Of transferred,for
R11625 T14649 T14647 arg2Of membranes,to
R11626 T14649 T14648 arg1Of membranes,PVDF
R11627 T14652 T14650 arg2Of blotting,for
R11628 T14652 T14651 arg1Of blotting,Western
R11629 T14655 T14653 arg2Of experiments,In
R11630 T14655 T14654 arg1Of experiments,some
R11631 T14657 T14658 arg1Of cells,were
R11632 T14657 T14659 arg2Of cells,cultured
R11633 T14659 T14653 arg1Of cultured,In
R11634 T14659 T14656 arg1Of cultured,","
R11635 T14659 T14658 arg2Of cultured,were
R11636 T14659 T14660 arg1Of cultured,for
R11637 T14662 T14660 arg2Of hrs,for
R11638 T14662 T14661 arg1Of hrs,6
R11639 T14662 T14663 arg1Of hrs,with
R11640 T14668 T14664 arg1Of peptide,25
R11641 T14668 T14665 arg1Of peptide,µM
R11642 T14668 T14666 arg1Of peptide,antennapedia-linked
R11643 T14668 T14667 arg1Of peptide,SMAC-N7
R11644 T14668 T14669 arg1Of peptide,(
R11645 T14668 T14672 arg1Of peptide,or
R11646 T14670 T14669 arg2Of Calbiochem,(
R11647 T14671 T14669 arg3Of ),(
R11648 T14672 T14663 arg2Of or,with
R11649 T14675 T14672 arg2Of volume,or
R11650 T14675 T14673 arg1Of volume,an
R11651 T14675 T14674 arg1Of volume,appropriate
R11652 T14675 T14676 arg1Of volume,of
R11653 T14678 T14676 arg2Of solvent,of
R11654 T14678 T14677 arg1Of solvent,the
R11655 T14678 T14679 arg2Of solvent,used
R11656 T14679 T14680 arg1Of used,for
R11657 T14682 T14680 arg2Of peptide,for
R11658 T14682 T14681 arg1Of peptide,the
R11659 T14682 T14683 arg1Of peptide,(
R11660 T14684 T14683 arg2Of DMSO,(
R11661 T14685 T14683 arg3Of ),(
R11662 T14687 T14686 arg2Of incubation,After
R11663 T14689 T14690 arg1Of cells,(
R11664 T14689 T14697 arg1Of cells,were
R11665 T14689 T14698 arg2Of cells,stimulated
R11666 T14691 T14690 arg2Of in,(
R11667 T14692 T14693 arg1Of peptide-,or
R11668 T14694 T14693 arg2Of DMSO-containing,or
R11669 T14695 T14691 arg2Of medium,in
R11670 T14695 T14692 arg1Of medium,peptide-
R11671 T14695 T14694 arg1Of medium,DMSO-containing
R11672 T14696 T14690 arg3Of ),(
R11673 T14698 T14686 arg1Of stimulated,After
R11674 T14698 T14688 arg1Of stimulated,","
R11675 T14698 T14697 arg2Of stimulated,were
R11676 T14698 T14699 arg1Of stimulated,with
R11677 T14698 T14702 arg1Of stimulated,as
R11678 T14701 T14699 arg2Of beads,with
R11679 T14701 T14700 arg1Of beads,antibody-coated
R11680 T14703 T14702 arg2Of outlined,as
R11681 T14703 T14704 arg1Of outlined,above
R11923 T15029 T15027 arg1Of targeting,HOIP
R11924 T15029 T15028 arg1Of targeting,gene
R11925 T15031 T15030 arg2Of A,(
R11926 T15032 T15030 arg3Of ),(
R11927 T15033 T15030 arg1Of Regions,(
R11928 T15033 T15034 arg1Of Regions,of
R11929 T15033 T15041 arg2Of Regions,used
R11930 T15033 T15051 arg1Of Regions,are
R11931 T15033 T15052 arg2Of Regions,shown
R11932 T15037 T15034 arg2Of sequence,of
R11933 T15037 T15035 arg1Of sequence,Rnf31
R11934 T15037 T15036 arg1Of sequence,gene
R11935 T15037 T15038 arg1Of sequence,(
R11936 T15039 T15038 arg2Of bottom,(
R11937 T15040 T15038 arg3Of ),(
R11938 T15041 T15042 arg1Of used,in
R11939 T15047 T15042 arg2Of vector,in
R11940 T15047 T15043 arg1Of vector,the
R11941 T15047 T15044 arg1Of vector,HOIP
R11942 T15047 T15045 arg1Of vector,gene
R11943 T15047 T15046 arg1Of vector,targeting
R11944 T15047 T15048 arg1Of vector,(
R11945 T15049 T15048 arg2Of top,(
R11946 T15050 T15048 arg3Of ),(
R11947 T15052 T15051 arg2Of shown,are
R11948 T15053 T15054 arg1Of Arrows,(
R11949 T15053 T15059 arg1Of Arrows,indicate
R11950 T15055 T15056 arg1Of F,and
R11951 T15056 T15054 arg2Of and,(
R11952 T15057 T15056 arg2Of R,and
R11953 T15058 T15054 arg3Of ),(
R11954 T15061 T15059 arg2Of positions,indicate
R11955 T15061 T15060 arg1Of positions,approximate
R11956 T15061 T15062 arg1Of positions,of
R11957 T15063 T15062 arg2Of sequences,of
R11958 T15063 T15064 arg1Of sequences,homologous
R11959 T15064 T15065 arg1Of homologous,to
R11960 T15066 T15065 arg2Of oligonucleotides,to
R11961 T15066 T15067 arg2Of oligonucleotides,used
R11962 T15067 T15068 arg1Of used,for
R11963 T15070 T15068 arg2Of detection,for
R11964 T15070 T15069 arg1Of detection,PCR-mediated
R11965 T15070 T15071 arg1Of detection,of
R11966 T15073 T15071 arg2Of recombination,of
R11967 T15073 T15072 arg1Of recombination,homologous
R11968 T15075 T15074 arg1Of recombination,Homologous
R11969 T15075 T15076 arg1Of recombination,of
R11970 T15075 T15081 arg1Of recombination,resulted
R11971 T15078 T15076 arg2Of vector,of
R11972 T15078 T15077 arg1Of vector,the
R11973 T15078 T15079 arg1Of vector,with
R11974 T15080 T15079 arg2Of Rnf31,with
R11975 T15081 T15082 arg1Of resulted,in
R11976 T15086 T15083 arg1Of deletion,a
R11977 T15086 T15084 arg1Of deletion,small
R11978 T15086 T15085 arg1Of deletion,chromosomal
R11979 T15086 T15087 arg1Of deletion,and
R11980 T15087 T15082 arg2Of and,in
R11981 T15090 T15087 arg2Of insertion,and
R11982 T15090 T15088 arg1Of insertion,the
R11983 T15090 T15089 arg1Of insertion,in-frame
R11984 T15090 T15091 arg1Of insertion,of
R11985 T15090 T15097 arg1Of insertion,into
R11986 T15093 T15091 arg2Of phosphotransferase,of
R11987 T15093 T15092 arg1Of phosphotransferase,neomycin
R11988 T15093 T15094 arg1Of phosphotransferase,(
R11989 T15095 T15094 arg2Of NeoR,(
R11990 T15096 T15094 arg3Of ),(
R11991 T15100 T15097 arg2Of HOIP,into
R11992 T15100 T15098 arg1Of HOIP,the
R11993 T15100 T15099 arg1Of HOIP,amino-terminal
R11994 T15100 T15101 arg1Of HOIP,coding
R11995 T15102 T15101 arg2Of sequence,coding
R11996 T15105 T15104 arg1Of toxin,diphtheria
R11997 T15105 T15106 arg1Of toxin,(
R11998 T15107 T15106 arg2Of DT,(
R11999 T15108 T15106 arg3Of ),(
R12000 T15109 T15103 arg1Of cassette,A
R12001 T15109 T15105 arg1Of cassette,toxin
R12002 T15109 T15110 arg1Of cassette,in
R12003 T15109 T15113 arg1Of cassette,facilitates
R12004 T15112 T15110 arg2Of vector,in
R12005 T15112 T15111 arg1Of vector,the
R12006 T15116 T15113 arg2Of selection,facilitates
R12007 T15116 T15114 arg1Of selection,the
R12008 T15116 T15115 arg1Of selection,negative
R12009 T15116 T15117 arg1Of selection,of
R12010 T15118 T15117 arg2Of cells,of
R12011 T15118 T15119 arg1Of cells,in
R12012 T15118 T15120 arg1Of cells,which
R12013 T15118 T15121 arg1Of cells,random
R12014 T15123 T15121 arg2Of insertion,random
R12015 T15123 T15122 arg1Of insertion,chromosomal
R12016 T15123 T15124 arg1Of insertion,of
R12017 T15123 T15127 arg1Of insertion,takes
R12018 T15126 T15124 arg2Of vector,of
R12019 T15126 T15125 arg1Of vector,the
R12020 T15127 T15121 arg3Of takes,random
R12021 T15128 T15127 arg2Of place,takes
R12022 T15130 T15129 arg1Of sequences,LoxP
R12023 T15130 T15131 arg1Of sequences,allow
R12024 T15134 T15131 arg2Of deletion,allow
R12025 T15134 T15132 arg1Of deletion,the
R12026 T15134 T15133 arg1Of deletion,Cre-mediated
R12027 T15134 T15135 arg1Of deletion,of
R12028 T15139 T15135 arg2Of sequence,of
R12029 T15139 T15136 arg1Of sequence,the
R12030 T15139 T15137 arg1Of sequence,NeoR
R12031 T15139 T15138 arg1Of sequence,coding
R12032 T15142 T15140 arg1Of sequence,The
R12033 T15142 T15141 arg1Of sequence,SV40pA
R12034 T15142 T15143 arg1Of sequence,helps
R12035 T15142 T15145 arg1Of sequence,ensure
R12036 T15145 T15143 arg2Of ensure,helps
R12037 T15145 T15144 arg1Of ensure,to
R12038 T15146 T15147 arg1Of disruption,of
R12039 T15146 T15150 arg1Of disruption,after
R12040 T15146 T15160 arg1Of disruption,and
R12041 T15149 T15147 arg2Of expression,of
R12042 T15149 T15148 arg1Of expression,gene
R12043 T15151 T15150 arg2Of deletion,after
R12044 T15151 T15152 arg1Of deletion,of
R12045 T15155 T15154 arg1Of sequence.,NeoR
R12046 T15155 T15156 arg1Of sequence.,(
R12047 T15157 T15156 arg2Of B,(
R12048 T15158 T15156 arg3Of ),(
R12049 T15159 T15152 arg2Of Anti-HOIP,of
R12050 T15159 T15153 arg1Of Anti-HOIP,the
R12051 T15159 T15155 arg1Of Anti-HOIP,sequence.
R12052 T15160 T15145 arg2Of and,ensure
R12053 T15163 T15160 arg2Of blots,and
R12054 T15163 T15161 arg1Of blots,anti-FLAG
R12055 T15163 T15162 arg1Of blots,Western
R12056 T15163 T15164 arg1Of blots,of
R12057 T15166 T15165 arg1Of lysates,cell
R12058 T15166 T15167 arg1Of lysates,from
R12059 T15166 T15170 arg1Of lysates,and
R12060 T15169 T15167 arg2Of cells,from
R12061 T15169 T15168 arg1Of cells,A20.2J
R12062 T15170 T15164 arg2Of and,of
R12063 T15171 T15172 arg1Of HOIP-deficient,(
R12064 T15173 T15172 arg2Of HOIP-/-,(
R12065 T15174 T15172 arg3Of ),(
R12066 T15175 T15170 arg2Of clones,and
R12067 T15175 T15171 arg1Of clones,HOIP-deficient
R12068 T15178 T15176 arg1Of decrease,A
R12069 T15178 T15177 arg1Of decrease,partial
R12070 T15178 T15179 arg1Of decrease,in
R12071 T15178 T15183 arg1Of decrease,is
R12072 T15178 T15184 arg1Of decrease,evident
R12073 T15182 T15179 arg2Of expression,in
R12074 T15182 T15180 arg1Of expression,HOIP
R12075 T15182 T15181 arg1Of expression,protein
R12076 T15183 T15185 arg1Of is,in
R12077 T15183 T15187 arg1Of is,following
R12078 T15184 T15183 arg2Of evident,is
R12079 T15186 T15185 arg2Of cells,in
R12080 T15188 T15187 arg2Of disruption,following
R12081 T15188 T15189 arg1Of disruption,of
R12082 T15191 T15189 arg2Of copy,of
R12083 T15191 T15190 arg1Of copy,one
R12084 T15191 T15192 arg1Of copy,of
R12085 T15193 T15192 arg2Of Rnf31,of
R12086 T15193 T15194 arg1Of Rnf31,(
R12087 T15195 T15194 arg2Of HOIP−/+,(
R12088 T15196 T15194 arg3Of ),(
R12089 T15198 T15197 arg1Of expression,HOIP
R12090 T15198 T15199 arg1Of expression,in
R12091 T15198 T15217 arg1Of expression,is
R12092 T15198 T15219 arg2Of expression,shown
R12093 T15200 T15199 arg2Of clones,in
R12094 T15200 T15201 arg2Of clones,reconstituted
R12095 T15201 T15202 arg1Of reconstituted,with
R12096 T15206 T15203 arg1Of vector,an
R12097 T15206 T15204 arg1Of vector,empty
R12098 T15206 T15205 arg1Of vector,retroviral
R12099 T15206 T15207 arg1Of vector,(
R12100 T15206 T15210 arg1Of vector,or
R12101 T15208 T15207 arg2Of pMIP,(
R12102 T15209 T15207 arg3Of ),(
R12103 T15210 T15202 arg2Of or,with
R12104 T15213 T15210 arg2Of vector,or
R12105 T15213 T15211 arg1Of vector,a
R12106 T15213 T15212 arg1Of vector,retroviral
R12107 T15213 T15214 arg1Of vector,encoding
R12108 T15216 T15214 arg2Of HOIP,encoding
R12109 T15216 T15215 arg1Of HOIP,FLAG-tagged
R12110 T15219 T15217 arg2Of shown,is
R12111 T15219 T15218 arg1Of shown,also
R12112 T15222 T15220 arg1Of weight,Approximate
R12113 T15222 T15221 arg1Of weight,molecular
R12114 T15222 T15223 arg1Of weight,of
R12115 T15222 T15225 arg1Of weight,is
R12116 T15224 T15223 arg2Of HOIP,of
R12117 T15227 T15225 arg2Of kD,is
R12118 T15227 T15226 arg1Of kD,120
R12278 T15492 T15490 arg1Of upregulation,CD40-mediated
R12279 T15492 T15491 arg1Of upregulation,CD80
R12280 T15492 T15493 arg1Of upregulation,is
R12281 T15492 T15494 arg1Of upregulation,defective
R12282 T15493 T15495 arg1Of is,in
R12283 T15494 T15493 arg2Of defective,is
R12284 T15497 T15495 arg2Of cells,in
R12285 T15497 T15496 arg1Of cells,HOIP-deficient
R12286 T15498 T15499 arg1Of Cells,were
R12287 T15498 T15500 arg2Of Cells,incubated
R12288 T15498 T15520 arg2Of Cells,stained
R12289 T15500 T15501 arg1Of incubated,for
R12290 T15500 T15518 arg1Of incubated,and
R12291 T15503 T15501 arg2Of hrs,for
R12292 T15503 T15502 arg1Of hrs,72
R12293 T15503 T15504 arg1Of hrs,with
R12294 T15508 T15506 arg1Of antibody,isotype
R12295 T15508 T15507 arg1Of antibody,control
R12296 T15508 T15509 arg1Of antibody,(
R12297 T15508 T15512 arg1Of antibody,or
R12298 T15510 T15509 arg2Of left,(
R12299 T15511 T15509 arg3Of ),(
R12300 T15512 T15504 arg2Of or,with
R12301 T15512 T15505 arg1Of or,an
R12302 T15514 T15512 arg2Of antibody,or
R12303 T15514 T15513 arg1Of antibody,anti-CD40
R12304 T15514 T15515 arg1Of antibody,(
R12305 T15516 T15515 arg2Of right,(
R12306 T15517 T15515 arg3Of ),(
R12307 T15518 T15499 arg2Of and,were
R12308 T15520 T15518 arg2Of stained,and
R12309 T15520 T15519 arg1Of stained,then
R12310 T15520 T15521 arg1Of stained,for
R12311 T15522 T15521 arg2Of expression,for
R12312 T15522 T15523 arg1Of expression,of
R12313 T15524 T15523 arg2Of CD80,of
R12314 T15524 T15525 arg1Of CD80,(
R12315 T15527 T15526 arg2Of profiles,filled
R12316 T15527 T15528 arg1Of profiles,indicate
R12317 T15528 T15525 arg2Of indicate,(
R12318 T15528 T15530 arg1Of indicate,with
R12319 T15528 T15533 arg1Of indicate,;
R12320 T15529 T15528 arg2Of staining,indicate
R12321 T15532 T15530 arg2Of antibody,with
R12322 T15532 T15531 arg1Of antibody,anti-CD80
R12323 T15535 T15534 arg1Of profiles,open
R12324 T15535 T15536 arg1Of profiles,are
R12325 T15535 T15537 arg1Of profiles,staining
R12326 T15537 T15533 arg2Of staining,;
R12327 T15537 T15536 arg2Of staining,are
R12328 T15537 T15538 arg1Of staining,with
R12329 T15542 T15538 arg2Of antibody,with
R12330 T15542 T15539 arg1Of antibody,an
R12331 T15542 T15540 arg1Of antibody,isotype
R12332 T15542 T15541 arg1Of antibody,control
R12333 T15543 T15525 arg3Of ),(
R12334 T15544 T15545 arg1Of Transduction,of
R12335 T15544 T15557 arg1Of Transduction,reversed
R12336 T15547 T15548 arg1Of HOIP-deficient,(
R12337 T15549 T15548 arg2Of HOIP-/-,(
R12338 T15550 T15548 arg3Of ),(
R12339 T15551 T15545 arg2Of clones,of
R12340 T15551 T15546 arg1Of clones,both
R12341 T15551 T15547 arg1Of clones,HOIP-deficient
R12342 T15551 T15552 arg1Of clones,with
R12343 T15554 T15552 arg2Of retrovirus,with
R12344 T15554 T15553 arg1Of retrovirus,a
R12345 T15554 T15555 arg1Of retrovirus,encoding
R12346 T15556 T15555 arg2Of FLAG-HOIP,encoding
R12347 T15557 T15561 arg1Of reversed,","
R12348 T15557 T15562 arg1Of reversed,while
R12349 T15560 T15557 arg2Of upregulation,reversed
R12350 T15560 T15558 arg1Of upregulation,defective
R12351 T15560 T15559 arg1Of upregulation,CD80
R12352 T15563 T15564 arg1Of transduction,with
R12353 T15563 T15571 arg1Of transduction,did
R12354 T15567 T15564 arg2Of vector,with
R12355 T15567 T15565 arg1Of vector,an
R12356 T15567 T15566 arg1Of vector,empty
R12357 T15567 T15568 arg1Of vector,(
R12358 T15569 T15568 arg2Of pMIP,(
R12359 T15570 T15568 arg3Of ),(
R12360 T15571 T15562 arg2Of did,while
R12361 T15571 T15572 arg1Of did,not
R12362 T15574 T15573 arg1Of results,Similar
R12363 T15574 T15575 arg1Of results,were
R12364 T15574 T15576 arg2Of results,obtained
R12365 T15576 T15575 arg2Of obtained,were
R12366 T15576 T15577 arg1Of obtained,in
R12367 T15580 T15577 arg2Of experiment,in
R12368 T15580 T15578 arg1Of experiment,a
R12369 T15580 T15579 arg1Of experiment,second
R12576 T15890 T15889 arg1Of transcription,GLε
R12577 T15890 T15891 arg1Of transcription,is
R12578 T15890 T15892 arg1Of transcription,defective
R12579 T15892 T15891 arg2Of defective,is
R12580 T15892 T15893 arg1Of defective,in
R12581 T15896 T15893 arg2Of cells,in
R12582 T15896 T15894 arg1Of cells,CD40-stimulated
R12583 T15896 T15895 arg1Of cells,HOIP-deficient
R12584 T15900 T15897 arg1Of cells,A20.2J
R12585 T15900 T15898 arg1Of cells,and
R12586 T15900 T15899 arg1Of cells,HOIP-deficient
R12587 T15900 T15901 arg2Of cells,transduced
R12588 T15900 T15907 arg1Of cells,(
R12589 T15900 T15919 arg1Of cells,or
R12590 T15901 T15902 arg1Of transduced,with
R12591 T15906 T15902 arg2Of vector,with
R12592 T15906 T15903 arg1Of vector,an
R12593 T15906 T15904 arg1Of vector,empty
R12594 T15906 T15905 arg1Of vector,retroviral
R12595 T15910 T15908 arg1Of pMIP,A20.2J
R12596 T15910 T15909 arg1Of pMIP,+
R12597 T15910 T15911 arg1Of pMIP,and
R12598 T15912 T15911 arg2Of HOIP-/-,and
R12599 T15914 T15907 arg2Of pMIP,(
R12600 T15914 T15910 arg1Of pMIP,pMIP
R12601 T15914 T15912 arg1Of pMIP,HOIP-/-
R12602 T15914 T15913 arg1Of pMIP,+
R12603 T15914 T15915 arg1Of pMIP,","
R12604 T15914 T15916 arg1Of pMIP,respectively
R12605 T15917 T15907 arg3Of ),(
R12606 T15919 T15918 arg1Of or,","
R12607 T15919 T15932 arg1Of or,were
R12608 T15919 T15933 arg2Of or,cultured
R12609 T15921 T15919 arg2Of cells,or
R12610 T15921 T15920 arg1Of cells,HOIP-deficient
R12611 T15921 T15922 arg2Of cells,transduced
R12612 T15922 T15923 arg1Of transduced,with
R12613 T15926 T15923 arg2Of retrovirus,with
R12614 T15926 T15924 arg1Of retrovirus,a
R12615 T15926 T15925 arg1Of retrovirus,HOIP-encoding
R12616 T15926 T15927 arg1Of retrovirus,(
R12617 T15930 T15927 arg2Of HOIP,(
R12618 T15930 T15928 arg1Of HOIP,HOIP-/-
R12619 T15930 T15929 arg1Of HOIP,+
R12620 T15931 T15927 arg3Of ),(
R12621 T15933 T15932 arg2Of cultured,were
R12622 T15933 T15934 arg1Of cultured,overnight
R12623 T15933 T15935 arg1Of cultured,with
R12624 T15933 T15946 arg1Of cultured,","
R12625 T15933 T15947 arg1Of cultured,with
R12626 T15933 T15949 arg1Of cultured,without
R12627 T15937 T15936 arg1Of anti-CD40,agonistic
R12628 T15937 T15938 arg1Of anti-CD40,or
R12629 T15938 T15935 arg2Of or,with
R12630 T15942 T15938 arg2Of antibody,or
R12631 T15942 T15939 arg1Of antibody,an
R12632 T15942 T15940 arg1Of antibody,isotype
R12633 T15942 T15941 arg1Of antibody,control
R12634 T15942 T15943 arg1Of antibody,(
R12635 T15944 T15943 arg2Of iso,(
R12636 T15945 T15943 arg3Of ),(
R12637 T15947 T15948 arg1Of with,or
R12638 T15949 T15948 arg2Of without,or
R12639 T15950 T15947 arg2Of IL-4,with
R12640 T15950 T15949 arg2Of IL-4,without
R12641 T15951 T15952 arg1Of RNA,was
R12642 T15951 T15953 arg2Of RNA,isolated
R12643 T15951 T15962 arg2Of RNA,subjected
R12644 T15953 T15954 arg1Of isolated,from
R12645 T15953 T15960 arg1Of isolated,and
R12646 T15956 T15954 arg2Of cells,from
R12647 T15956 T15955 arg1Of cells,the
R12648 T15956 T15957 arg1Of cells,","
R12649 T15958 T15957 arg2Of reverse-transcribed,","
R12650 T15960 T15952 arg2Of and,was
R12651 T15960 T15959 arg1Of and,","
R12652 T15962 T15960 arg2Of subjected,and
R12653 T15962 T15961 arg1Of subjected,then
R12654 T15962 T15963 arg1Of subjected,to
R12655 T15962 T15966 modOf subjected,to
R12656 T15965 T15963 arg2Of PCR,to
R12657 T15965 T15964 arg1Of PCR,quantitative
R12658 T15967 T15966 arg1Of determine,to
R12659 T15968 T15967 arg2Of levels,determine
R12660 T15968 T15969 arg1Of levels,of
R12661 T15971 T15969 arg2Of transcripts,of
R12662 T15971 T15970 arg1Of transcripts,GLε
R12663 T15972 T15973 arg1Of Results,were
R12664 T15972 T15974 arg2Of Results,normalized
R12665 T15974 T15973 arg2Of normalized,were
R12666 T15974 T15975 arg1Of normalized,to
R12667 T15977 T15975 arg2Of levels,to
R12668 T15977 T15976 arg1Of levels,the
R12669 T15977 T15978 arg1Of levels,of
R12670 T15977 T15981 arg1Of levels,in
R12671 T15980 T15978 arg2Of transcripts,of
R12672 T15980 T15979 arg1Of transcripts,Hprt1
R12673 T15983 T15981 arg2Of sample,in
R12674 T15983 T15982 arg1Of sample,each
R12675 T15984 T15985 arg1Of Symbols,indicate
R12676 T15985 T15991 arg1Of indicate,(
R12677 T15987 T15985 arg2Of values,indicate
R12678 T15987 T15986 arg1Of values,the
R12679 T15987 T15988 arg1Of values,from
R12680 T15990 T15988 arg2Of cultures,from
R12681 T15990 T15989 arg1Of cultures,duplicate
R12682 T15993 T15992 arg1Of line,a
R12683 T15993 T15994 arg1Of line,indicates
R12684 T15994 T15991 arg2Of indicates,(
R12685 T15996 T15994 arg2Of mean,indicates
R12686 T15996 T15995 arg1Of mean,the
R12687 T15996 T15997 arg1Of mean,of
R12688 T16000 T15997 arg2Of values,of
R12689 T16000 T15998 arg1Of values,the
R12690 T16000 T15999 arg1Of values,two
R12691 T16001 T15991 arg3Of ),(
R12692 T16003 T16002 arg1Of results,Similar
R12693 T16003 T16004 arg1Of results,were
R12694 T16003 T16005 arg2Of results,obtained
R12695 T16005 T16004 arg2Of obtained,were
R12696 T16005 T16006 arg1Of obtained,in
R12697 T16005 T16011 arg1Of obtained,in
R12698 T16005 T16015 arg1Of obtained,with
R12699 T16006 T16010 arg1Of in,and
R12700 T16009 T16006 arg2Of experiment,in
R12701 T16009 T16007 arg1Of experiment,a
R12702 T16009 T16008 arg1Of experiment,second
R12703 T16011 T16010 arg2Of in,and
R12704 T16014 T16011 arg2Of experiment,in
R12705 T16014 T16012 arg1Of experiment,an
R12706 T16014 T16013 arg1Of experiment,additional
R12707 T16019 T16015 arg2Of clone,with
R12708 T16019 T16016 arg1Of clone,a
R12709 T16019 T16017 arg1Of clone,second
R12710 T16019 T16018 arg1Of clone,HOIP-deficient
R12939 T16375 T16374 arg1Of activation,CD40-induced
R12940 T16375 T16376 arg1Of activation,of
R12941 T16375 T16380 arg1Of activation,is
R12942 T16375 T16381 arg1Of activation,defective
R12943 T16377 T16378 arg1Of NF-κB,and
R12944 T16378 T16376 arg2Of and,of
R12945 T16379 T16378 arg2Of JNK,and
R12946 T16380 T16382 arg1Of is,in
R12947 T16381 T16380 arg2Of defective,is
R12948 T16384 T16382 arg2Of cells,in
R12949 T16384 T16383 arg1Of cells,HOIP-deficient
R12950 T16386 T16385 arg1Of signaling,CD40-mediated
R12951 T16386 T16387 arg1Of signaling,was
R12952 T16386 T16388 arg1Of signaling,defective
R12953 T16386 T16399 arg1Of signaling,intact
R12954 T16388 T16389 arg1Of defective,in
R12955 T16388 T16398 arg1Of defective,but
R12956 T16391 T16389 arg2Of cells,in
R12957 T16391 T16390 arg1Of cells,HOIP-deficient
R12958 T16391 T16392 arg1Of cells,(
R12959 T16393 T16392 arg2Of HOIP-/-,(
R12960 T16393 T16394 arg1Of HOIP-/-,+
R12961 T16393 T16395 arg1Of HOIP-/-,pMIP
R12962 T16396 T16392 arg3Of ),(
R12963 T16398 T16387 arg2Of but,was
R12964 T16398 T16397 arg1Of but,","
R12965 T16399 T16398 arg2Of intact,but
R12966 T16399 T16400 arg1Of intact,in
R12967 T16402 T16400 arg2Of cells,in
R12968 T16402 T16401 arg1Of cells,parental
R12969 T16402 T16403 arg2Of cells,transduced
R12970 T16403 T16404 arg1Of transduced,with
R12971 T16403 T16415 arg1Of transduced,in
R12972 T16404 T16414 arg1Of with,and
R12973 T16408 T16404 arg2Of vector,with
R12974 T16408 T16405 arg1Of vector,an
R12975 T16408 T16406 arg1Of vector,empty
R12976 T16408 T16407 arg1Of vector,retroviral
R12977 T16408 T16409 arg1Of vector,(
R12978 T16410 T16409 arg2Of A20.2J,(
R12979 T16410 T16411 arg1Of A20.2J,+
R12980 T16410 T16412 arg1Of A20.2J,pMIP
R12981 T16413 T16409 arg3Of ),(
R12982 T16415 T16414 arg2Of in,and
R12983 T16417 T16415 arg2Of cells,in
R12984 T16417 T16416 arg1Of cells,HOIP-deficient
R12985 T16417 T16418 arg2Of cells,transduced
R12986 T16418 T16419 arg1Of transduced,with
R12987 T16422 T16419 arg2Of vector,with
R12988 T16422 T16420 arg1Of vector,a
R12989 T16422 T16421 arg1Of vector,retroviral
R12990 T16422 T16423 arg1Of vector,encoding
R12991 T16424 T16423 arg2Of HOIP,encoding
R12992 T16424 T16425 arg1Of HOIP,(
R12993 T16426 T16425 arg2Of HOIP-/-,(
R12994 T16426 T16427 arg1Of HOIP-/-,+
R12995 T16426 T16428 arg1Of HOIP-/-,HOIP
R12996 T16429 T16425 arg3Of ),(
R12997 T16430 T16431 arg1Of Cells,were
R12998 T16430 T16432 arg2Of Cells,activated
R12999 T16432 T16431 arg2Of activated,were
R13000 T16432 T16433 arg1Of activated,with
R13001 T16432 T16440 arg2Of activated,indicated
R13002 T16435 T16433 arg2Of insect,with
R13003 T16435 T16434 arg1Of insect,CD154-expressing
R13004 T16436 T16437 arg1Of cells,for
R13005 T16436 T16440 arg1Of cells,indicated
R13006 T16439 T16437 arg2Of times,for
R13007 T16439 T16438 arg1Of times,the
R13008 T16444 T16441 arg2Of control,As
R13009 T16444 T16442 arg1Of control,a
R13010 T16444 T16443 arg1Of control,negative
R13011 T16446 T16447 arg1Of cells,were
R13012 T16446 T16448 arg2Of cells,stimulated
R13013 T16448 T16441 arg1Of stimulated,As
R13014 T16448 T16445 arg1Of stimulated,","
R13015 T16448 T16447 arg2Of stimulated,were
R13016 T16448 T16449 arg1Of stimulated,with
R13017 T16451 T16449 arg2Of cells,with
R13018 T16451 T16450 arg1Of cells,insect
R13019 T16451 T16452 arg1Of cells,lacking
R13020 T16453 T16452 arg2Of CD154,lacking
R13021 T16453 T16454 arg1Of CD154,(
R13022 T16458 T16454 arg2Of point,(
R13023 T16458 T16455 arg1Of point,5
R13024 T16458 T16456 arg1Of point,minute
R13025 T16458 T16457 arg1Of point,time
R13026 T16458 T16459 arg1Of point,only
R13027 T16460 T16454 arg3Of ),(
R13028 T16462 T16461 arg1Of blots,Western
R13029 T16462 T16463 arg1Of blots,of
R13030 T16462 T16466 arg1Of blots,were
R13031 T16462 T16467 arg2Of blots,probed
R13032 T16465 T16463 arg2Of lysates,of
R13033 T16465 T16464 arg1Of lysates,whole-cell
R13034 T16467 T16466 arg2Of probed,were
R13035 T16467 T16468 arg1Of probed,with
R13036 T16471 T16468 arg2Of antibodies,with
R13037 T16471 T16469 arg1Of antibodies,the
R13038 T16471 T16470 arg1Of antibodies,indicated
R13039 T16472 T16473 arg1Of Phospho-IκBα,(
R13040 T16472 T16476 arg1Of Phospho-IκBα,and
R13041 T16474 T16473 arg2Of pIκBα,(
R13042 T16475 T16473 arg3Of ),(
R13043 T16476 T16482 arg1Of and,were
R13044 T16476 T16483 arg2Of and,stripped
R13045 T16476 T16485 arg2Of and,reprobed
R13046 T16479 T16478 arg2Of pJNK,(
R13047 T16480 T16478 arg3Of ),(
R13048 T16481 T16476 arg2Of blots,and
R13049 T16481 T16477 arg1Of blots,phospho-JNK
R13050 T16481 T16478 arg1Of blots,(
R13051 T16483 T16484 arg1Of stripped,and
R13052 T16484 T16482 arg2Of and,were
R13053 T16484 T16486 arg1Of and,for
R13054 T16484 T16492 arg1Of and,","
R13055 T16485 T16484 arg2Of reprobed,and
R13056 T16488 T16487 arg1Of IκBα,total
R13057 T16488 T16489 arg1Of IκBα,and
R13058 T16489 T16486 arg2Of and,for
R13059 T16491 T16489 arg2Of JNK,and
R13060 T16491 T16490 arg1Of JNK,total
R13061 T16496 T16495 arg1Of antibodies,anti-JNK
R13062 T16496 T16497 arg1Of antibodies,recognize
R13063 T16497 T16492 arg2Of recognize,","
R13064 T16497 T16493 arg1Of recognize,respectively
R13065 T16497 T16494 arg1Of recognize,(
R13066 T16497 T16502 arg1Of recognize,)
R13067 T16498 T16499 arg1Of p46,and
R13068 T16500 T16499 arg2Of p54,and
R13069 T16501 T16497 arg2Of isoforms,recognize
R13070 T16501 T16498 arg1Of isoforms,p46
R13071 T16501 T16500 arg1Of isoforms,p54
R13072 T16504 T16503 arg1Of blots,IκBα
R13073 T16504 T16505 arg1Of blots,were
R13074 T16504 T16507 arg2Of blots,reprobed
R13075 T16504 T16511 arg1Of blots,demonstrate
R13076 T16507 T16505 arg2Of reprobed,were
R13077 T16507 T16506 arg1Of reprobed,also
R13078 T16507 T16508 arg1Of reprobed,for
R13079 T16509 T16508 arg2Of actin,for
R13080 T16511 T16507 arg3Of demonstrate,reprobed
R13081 T16511 T16510 arg1Of demonstrate,to
R13082 T16514 T16511 arg2Of loading,demonstrate
R13083 T16514 T16512 arg1Of loading,equal
R13084 T16514 T16513 arg1Of loading,lane
R13085 T16516 T16515 arg1Of weights,Molecular
R13086 T16516 T16517 arg1Of weights,are
R13087 T16516 T16518 arg2Of weights,indicated
R13088 T16518 T16517 arg2Of indicated,are
R13089 T16518 T16519 arg1Of indicated,at
R13090 T16520 T16519 arg2Of right,at
R13091 T16522 T16521 arg1Of results,Similar
R13092 T16522 T16523 arg1Of results,were
R13093 T16522 T16524 arg2Of results,obtained
R13094 T16524 T16523 arg2Of obtained,were
R13095 T16524 T16525 arg1Of obtained,in
R13096 T16524 T16530 arg1Of obtained,in
R13097 T16524 T16534 arg1Of obtained,with
R13098 T16525 T16529 arg1Of in,and
R13099 T16528 T16525 arg2Of experiment,in
R13100 T16528 T16526 arg1Of experiment,a
R13101 T16528 T16527 arg1Of experiment,second
R13102 T16530 T16529 arg2Of in,and
R13103 T16533 T16530 arg2Of experiments,in
R13104 T16533 T16531 arg1Of experiments,two
R13105 T16533 T16532 arg1Of experiments,additional
R13106 T16538 T16534 arg2Of clone,with
R13107 T16538 T16535 arg1Of clone,a
R13108 T16538 T16536 arg1Of clone,second
R13109 T16538 T16537 arg1Of clone,HOIP-deficient
R1343 T1726 T1725 arg1Of signaling,CD40
R1344 T1726 T1727 arg1Of signaling,in
R1345 T1726 T1742 arg1Of signaling,is
R1346 T1726 T1743 arg1Of signaling,critical
R1347 T1730 T1727 arg2Of cells,in
R1348 T1730 T1728 arg1Of cells,professional
R1349 T1730 T1729 arg1Of cells,antigen-presenting
R1350 T1730 T1731 arg1Of cells,","
R1351 T1730 T1732 arg1Of cells,including
R1352 T1734 T1733 arg1Of cells,B
R1353 T1734 T1735 arg1Of cells,","
R1354 T1735 T1738 arg1Of ",",and
R1355 T1736 T1735 arg2Of macrophages,","
R1356 T1738 T1732 arg2Of and,including
R1357 T1738 T1737 arg1Of and,","
R1358 T1740 T1738 arg2Of cells,and
R1359 T1740 T1739 arg1Of cells,dendritic
R1360 T1742 T1741 arg1Of is,","
R1361 T1742 T1757 arg1Of is,","
R1362 T1742 T1758 arg1Of is,[
R1363 T1742 T1761 arg1Of is,","
R13634 T17282 T17281 arg1Of recruitment,IKK
R13635 T17282 T17283 arg1Of recruitment,to
R13636 T17282 T17288 arg1Of recruitment,is
R13637 T17282 T17289 arg1Of recruitment,defective
R13638 T17287 T17283 arg2Of complex,to
R13639 T17287 T17284 arg1Of complex,the
R1364 T1742 T1762 arg1Of is,[
R13640 T17287 T17285 arg1Of complex,CD40
R13641 T17287 T17286 arg1Of complex,signaling
R13642 T17288 T17290 arg1Of is,in
R13643 T17289 T17288 arg2Of defective,is
R13644 T17292 T17290 arg2Of cells,in
R13645 T17292 T17291 arg1Of cells,HOIP-deficient
R13646 T17294 T17293 arg2Of A,(
R13647 T17295 T17293 arg3Of ),(
R13648 T17298 T17293 arg1Of treatment,(
R13649 T17298 T17296 arg1Of treatment,SMAC
R1365 T1743 T1742 arg2Of critical,is
R13650 T17298 T17297 arg1Of treatment,peptide
R13651 T17298 T17299 arg1Of treatment,reduces
R13652 T17298 T17306 arg1Of treatment,may
R13653 T17298 T17307 arg1Of treatment,modify
R13654 T17299 T17305 arg1Of reduces,and
R13655 T17300 T17299 arg2Of recruitment,reduces
R13656 T17300 T17301 arg1Of recruitment,of
R13657 T17300 T17303 arg1Of recruitment,to
R13658 T17302 T17301 arg2Of cIAP1,of
R13659 T17304 T17303 arg2Of CD40,to
R1366 T1743 T1744 arg1Of critical,for
R13660 T17307 T17305 arg2Of modify,and
R13661 T17307 T17306 arg2Of modify,may
R13662 T17309 T17307 arg2Of recruitment,modify
R13663 T17309 T17308 arg1Of recruitment,HOIP
R13664 T17311 T17310 arg1Of cells,A20.2J
R13665 T17311 T17312 arg1Of cells,were
R13666 T17311 T17313 arg2Of cells,incubated
R13667 T17313 T17312 arg2Of incubated,were
R13668 T17313 T17314 arg1Of incubated,for
R13669 T17316 T17314 arg2Of hours,for
R1367 T1747 T1744 arg2Of activation,for
R13670 T17316 T17315 arg1Of hours,six
R13671 T17316 T17317 arg1Of hours,with
R13672 T17320 T17318 arg1Of peptide,membrane-permeable
R13673 T17320 T17319 arg1Of peptide,SMAC-N7
R13674 T17320 T17321 arg1Of peptide,or
R13675 T17321 T17317 arg2Of or,with
R13676 T17322 T17323 arg1Of 1.5,%
R13677 T17324 T17321 arg2Of DMSO,or
R13678 T17324 T17322 arg1Of DMSO,1.5
R13679 T17324 T17325 arg1Of DMSO,(
R1368 T1747 T1745 arg1Of activation,the
R13680 T17326 T17325 arg2Of solvent,(
R13681 T17326 T17327 arg2Of solvent,used
R13682 T17327 T17328 arg1Of used,for
R13683 T17330 T17328 arg2Of peptide,for
R13684 T17330 T17329 arg1Of peptide,the
R13685 T17331 T17325 arg3Of ),(
R13686 T17334 T17332 arg2Of incubation,Following
R13687 T17334 T17333 arg1Of incubation,the
R13688 T17337 T17336 arg1Of lysates,cell
R13689 T17337 T17338 arg1Of lysates,were
R1369 T1747 T1746 arg1Of activation,efficient
R13690 T17337 T17339 arg2Of lysates,prepared
R13691 T17337 T17341 arg2Of lysates,fractionated
R13692 T17337 T17346 arg2Of lysates,evaluated
R13693 T17339 T17340 arg1Of prepared,","
R13694 T17340 T17345 arg1Of ",",and
R13695 T17341 T17340 arg2Of fractionated,","
R13696 T17343 T17341 arg1Of SDS-PAGE,fractionated
R13697 T17343 T17342 arg2Of SDS-PAGE,by
R13698 T17345 T17332 arg1Of and,Following
R13699 T17345 T17335 arg1Of and,","
R1370 T1747 T1748 arg1Of activation,of
R13700 T17345 T17338 arg2Of and,were
R13701 T17345 T17344 arg1Of and,","
R13702 T17346 T17345 arg2Of evaluated,and
R13703 T17349 T17346 arg1Of blot,evaluated
R13704 T17349 T17347 arg2Of blot,by
R13705 T17349 T17348 arg1Of blot,Western
R13706 T17349 T17350 arg1Of blot,(
R13707 T17352 T17353 arg1Of 1,and
R13708 T17353 T17350 arg2Of and,(
R13709 T17353 T17351 arg1Of and,lanes
R1371 T1749 T1750 arg1Of humoral,and
R13710 T17354 T17353 arg2Of 2,and
R13711 T17355 T17350 arg3Of ),(
R13712 T17356 T17357 arg2Of Cells,incubated
R13713 T17356 T17362 arg1Of Cells,were
R13714 T17356 T17364 arg2Of Cells,stimulated
R13715 T17357 T17358 arg1Of incubated,with
R13716 T17359 T17360 arg1Of DMSO,or
R13717 T17360 T17358 arg2Of or,with
R13718 T17361 T17360 arg2Of SMAC-N7,or
R13719 T17364 T17362 arg2Of stimulated,were
R1372 T1751 T1750 arg2Of cell-mediated,and
R13720 T17364 T17363 arg1Of stimulated,also
R13721 T17364 T17365 arg1Of stimulated,with
R13722 T17367 T17365 arg2Of beads,with
R13723 T17367 T17366 arg1Of beads,magnetic
R13724 T17367 T17368 arg2Of beads,coated
R13725 T17368 T17369 arg1Of coated,with
R13726 T17370 T17371 arg1Of anti-CD40,or
R13727 T17371 T17369 arg2Of or,with
R13728 T17375 T17371 arg2Of antibody,or
R13729 T17375 T17372 arg1Of antibody,an
R1373 T1753 T1748 arg2Of responses,of
R13730 T17375 T17373 arg1Of antibody,isotype
R13731 T17375 T17374 arg1Of antibody,control
R13732 T17378 T17377 arg2Of IP,(
R13733 T17379 T17377 arg3Of ),(
R13734 T17380 T17376 arg2Of material,Immunoprecipitated
R13735 T17380 T17377 arg1Of material,(
R13736 T17380 T17381 arg2Of material,bound
R13737 T17380 T17385 arg1Of material,was
R13738 T17380 T17386 arg2Of material,loaded
R13739 T17381 T17382 arg1Of bound,to
R1374 T1753 T1749 arg1Of responses,humoral
R13740 T17384 T17382 arg2Of beads,to
R13741 T17384 T17383 arg1Of beads,the
R13742 T17386 T17385 arg2Of loaded,was
R13743 T17386 T17387 arg1Of loaded,in
R13744 T17388 T17387 arg2Of lanes,in
R13745 T17388 T17389 arg1Of lanes,3-5
R13746 T17390 T17391 arg1Of Samples,of
R13747 T17390 T17397 arg1Of Samples,appear
R13748 T17394 T17391 arg2Of lysates,of
R13749 T17394 T17392 arg1Of lysates,the
R1375 T1753 T1751 arg1Of responses,cell-mediated
R13750 T17394 T17393 arg1Of lysates,cell
R13751 T17394 T17395 arg1Of lysates,after
R13752 T17396 T17395 arg2Of immunoprecipitation,after
R13753 T17397 T17398 arg1Of appear,in
R13754 T17399 T17398 arg2Of lanes,in
R13755 T17399 T17400 arg1Of lanes,6–8
R13756 T17402 T17401 arg1Of blots,Western
R13757 T17402 T17403 arg1Of blots,were
R13758 T17402 T17404 arg2Of blots,probed
R13759 T17404 T17403 arg2Of probed,were
R1376 T1753 T1752 arg1Of responses,immune
R13760 T17404 T17405 arg1Of probed,with
R13761 T17406 T17405 arg2Of antibodies,with
R13762 T17406 T17407 arg1Of antibodies,specific
R13763 T17407 T17408 arg1Of specific,for
R13764 T17409 T17410 arg1Of cIAP1,","
R13765 T17410 T17412 arg1Of ",",","
R13766 T17411 T17410 arg2Of TRAF2,","
R13767 T17412 T17415 arg1Of ",",and
R13768 T17413 T17412 arg2Of TRAF3,","
R13769 T17415 T17408 arg2Of and,for
R1377 T1753 T1754 arg1Of responses,[
R13770 T17415 T17414 arg1Of and,","
R13771 T17416 T17415 arg2Of HOIP,and
R13772 T17416 T17417 arg1Of HOIP,(
R13773 T17420 T17418 arg1Of weights,approximate
R13774 T17420 T17419 arg1Of weights,molecular
R13775 T17420 T17421 arg1Of weights,indicated
R13776 T17421 T17417 arg2Of indicated,(
R13777 T17421 T17422 arg1Of indicated,on
R13778 T17423 T17422 arg2Of left,on
R13779 T17424 T17417 arg3Of ),(
R1378 T1755 T1754 arg2Of 1,[
R13780 T17426 T17425 arg1Of results,Similar
R13781 T17426 T17427 arg1Of results,were
R13782 T17426 T17428 arg2Of results,obtained
R13783 T17428 T17427 arg2Of obtained,were
R13784 T17428 T17429 arg1Of obtained,in
R13785 T17432 T17429 arg2Of experiments.,in
R13786 T17432 T17430 arg1Of experiments.,two
R13787 T17432 T17431 arg1Of experiments.,additional
R13788 T17432 T17433 arg1Of experiments.,(
R13789 T17432 T17454 arg2Of experiments.,transduced
R1379 T1756 T1754 arg3Of ],[
R13790 T17434 T17433 arg2Of B,(
R13791 T17435 T17433 arg3Of ),(
R13792 T17436 T17437 arg1Of CD40,was
R13793 T17436 T17438 arg2Of CD40,isolated
R13794 T17438 T17437 arg2Of isolated,was
R13795 T17438 T17439 arg1Of isolated,by
R13796 T17440 T17439 arg2Of immunoprecipitation,by
R13797 T17440 T17441 arg1Of immunoprecipitation,(
R13798 T17440 T17448 arg1Of immunoprecipitation,from
R13799 T17443 T17441 arg2Of in,(
R1380 T1759 T1758 arg2Of 2,[
R13800 T17443 T17442 arg1Of in,as
R13801 T17445 T17443 arg2Of A,in
R13802 T17445 T17444 arg2Of A,(
R13803 T17446 T17444 arg3Of ),(
R13804 T17447 T17441 arg3Of ),(
R13805 T17450 T17449 arg1Of cells,A20.2J
R13806 T17450 T17451 arg1Of cells,and
R13807 T17451 T17448 arg2Of and,from
R13808 T17453 T17451 arg2Of cells,and
R13809 T17453 T17452 arg1Of cells,HOIP-deficient
R1381 T1760 T1758 arg3Of ],[
R13810 T17454 T17437 modOf transduced,was
R13811 T17454 T17455 arg1Of transduced,with
R13812 T17459 T17456 arg1Of vector,an
R13813 T17459 T17457 arg1Of vector,empty
R13814 T17459 T17458 arg1Of vector,retroviral
R13815 T17459 T17460 arg1Of vector,(
R13816 T17459 T17463 arg1Of vector,or
R13817 T17461 T17460 arg2Of pMIP,(
R13818 T17462 T17460 arg3Of ),(
R13819 T17463 T17455 arg2Of or,with
R1382 T1763 T1762 arg2Of 3,[
R13820 T17466 T17463 arg2Of vector,or
R13821 T17466 T17464 arg1Of vector,a
R13822 T17466 T17465 arg1Of vector,retroviral
R13823 T17466 T17467 arg1Of vector,encoding
R13824 T17468 T17467 arg2Of HOIP,encoding
R13825 T17469 T17470 arg1Of Material,immunoprecipitated
R13826 T17470 T17471 arg1Of immunoprecipitated,with
R13827 T17470 T17479 arg1Of immunoprecipitated,or
R13828 T17475 T17471 arg2Of antibody,with
R13829 T17475 T17472 arg1Of antibody,an
R1383 T1764 T1762 arg3Of ],[
R13830 T17475 T17473 arg1Of antibody,isotype
R13831 T17475 T17474 arg1Of antibody,control
R13832 T17475 T17476 arg1Of antibody,(
R13833 T17477 T17476 arg2Of isotype,(
R13834 T17478 T17476 arg3Of ),(
R13835 T17481 T17480 arg1Of antibody,anti-CD40
R13836 T17481 T17482 arg1Of antibody,was
R13837 T17481 T17483 arg2Of antibody,examined
R13838 T17483 T17479 arg2Of examined,or
R13839 T17483 T17482 arg2Of examined,was
R1384 T1766 T1765 arg1Of signaling,CD40
R13840 T17486 T17483 arg1Of blotting,examined
R13841 T17486 T17484 arg2Of blotting,by
R13842 T17486 T17485 arg1Of blotting,Western
R13843 T17486 T17487 arg1Of blotting,for
R13844 T17488 T17489 arg1Of CD40,","
R13845 T17489 T17491 arg1Of ",",","
R13846 T17490 T17489 arg2Of TRAF2,","
R13847 T17491 T17493 arg1Of ",",","
R13848 T17492 T17491 arg2Of TRAF3,","
R13849 T17493 T17495 arg1Of ",",","
R1385 T1766 T1767 arg1Of signaling,is
R13850 T17494 T17493 arg2Of cIAP1,","
R13851 T17495 T17497 arg1Of ",",","
R13852 T17496 T17495 arg2Of HOIP,","
R13853 T17497 T17500 arg1Of ",",and
R13854 T17498 T17497 arg2Of IKKα/β,","
R13855 T17500 T17487 arg2Of and,for
R13856 T17500 T17499 arg1Of and,","
R13857 T17501 T17500 arg2Of IKKγ,and
R13858 T17501 T17502 arg1Of IKKγ,(
R13859 T17504 T17502 arg2Of panels,(
R1386 T1766 T1768 arg2Of signaling,activated
R13860 T17504 T17503 arg1Of panels,right
R13861 T17505 T17502 arg3Of ),(
R13862 T17507 T17506 arg1Of expression,HOIP
R13863 T17507 T17508 arg1Of expression,was
R13864 T17507 T17509 arg2Of expression,required
R13865 T17509 T17508 arg2Of required,was
R13866 T17509 T17510 arg1Of required,for
R13867 T17511 T17510 arg2Of coprecipitation,for
R13868 T17511 T17512 arg1Of coprecipitation,of
R13869 T17514 T17512 arg2Of proteins,of
R1387 T1768 T1767 arg2Of activated,is
R13870 T17514 T17513 arg1Of proteins,IKK
R13871 T17514 T17515 arg1Of proteins,with
R13872 T17516 T17515 arg2Of CD40,with
R13873 T17518 T17517 arg1Of lysates,Cell
R13874 T17518 T17519 arg1Of lysates,from
R13875 T17518 T17522 arg1Of lysates,are
R13876 T17518 T17523 arg2Of lysates,shown
R13877 T17521 T17519 arg2Of cells,from
R13878 T17521 T17520 arg1Of cells,unstimulated
R13879 T17523 T17522 arg2Of shown,are
R1388 T1768 T1769 arg1Of activated,in
R13880 T17523 T17524 arg1Of shown,in
R13881 T17527 T17524 arg2Of panels,in
R13882 T17527 T17525 arg1Of panels,the
R13883 T17527 T17526 arg1Of panels,left
R13884 T17529 T17528 arg1Of results,Similar
R13885 T17529 T17530 arg1Of results,were
R13886 T17529 T17531 arg2Of results,obtained
R13887 T17531 T17530 arg2Of obtained,were
R13888 T17531 T17532 arg1Of obtained,in
R13889 T17531 T17537 arg1Of obtained,in
R1389 T1768 T1774 arg1Of activated,","
R13890 T17531 T17540 arg1Of obtained,with
R13891 T17532 T17536 arg1Of in,and
R13892 T17535 T17532 arg2Of experiment,in
R13893 T17535 T17533 arg1Of experiment,a
R13894 T17535 T17534 arg1Of experiment,second
R13895 T17537 T17536 arg2Of in,and
R13896 T17539 T17537 arg2Of experiments,in
R13897 T17539 T17538 arg1Of experiments,two
R13898 T17544 T17540 arg2Of clone.,with
R13899 T17544 T17541 arg1Of clone.,a
R1390 T1768 T1775 arg1Of activated,as
R13900 T17544 T17542 arg1Of clone.,second
R13901 T17544 T17543 arg1Of clone.,HOIP-deficient
R13902 T17544 T17545 arg1Of clone.,(
R13903 T17544 T17548 modOf clone.,To
R13904 T17546 T17545 arg2Of C,(
R13905 T17547 T17545 arg3Of ),(
R13906 T17550 T17548 arg1Of evaluate,To
R13907 T17550 T17549 arg1Of evaluate,further
R13908 T17552 T17550 arg2Of recruitment,evaluate
R13909 T17552 T17551 arg1Of recruitment,HOIP-dependent
R1391 T1773 T1769 arg2Of manner,in
R13910 T17552 T17553 arg1Of recruitment,of
R13911 T17552 T17555 arg1Of recruitment,to
R13912 T17554 T17553 arg2Of IKKγ,of
R13913 T17556 T17555 arg2Of CD40,to
R13914 T17559 T17558 arg1Of cells,A20.2J
R13915 T17559 T17560 arg1Of cells,or
R13916 T17560 T17567 arg1Of or,were
R13917 T17560 T17568 arg2Of or,transduced
R13918 T17561 T17562 arg1Of HOIP-deficient,(
R13919 T17563 T17562 arg2Of HOIP-/-,(
R1392 T1773 T1770 arg1Of manner,a
R13920 T17564 T17562 arg3Of ),(
R13921 T17566 T17560 arg2Of cells,or
R13922 T17566 T17561 arg1Of cells,HOIP-deficient
R13923 T17566 T17565 arg1Of cells,A20.2J
R13924 T17568 T17530 modOf transduced,were
R13925 T17568 T17557 arg1Of transduced,","
R13926 T17568 T17567 arg2Of transduced,were
R13927 T17568 T17569 arg1Of transduced,with
R13928 T17573 T17570 arg1Of vector,an
R13929 T17573 T17571 arg1Of vector,empty
R1393 T1773 T1771 arg1Of manner,T
R13930 T17573 T17572 arg1Of vector,retroviral
R13931 T17573 T17574 arg1Of vector,or
R13932 T17574 T17569 arg2Of or,with
R13933 T17577 T17574 arg2Of construct,or
R13934 T17577 T17575 arg1Of construct,a
R13935 T17577 T17576 arg1Of construct,retroviral
R13936 T17577 T17578 arg1Of construct,encoding
R13937 T17581 T17578 arg2Of IKKγ,encoding
R13938 T17581 T17579 arg1Of IKKγ,BP
R13939 T17581 T17580 arg1Of IKKγ,epitope-tagged
R1394 T1773 T1772 arg1Of manner,cell-dependent
R13940 T17581 T17582 arg1Of IKKγ,(
R13941 T17583 T17582 arg2Of noted,(
R13942 T17583 T17584 arg1Of noted,in
R13943 T17583 T17587 arg1Of noted,as
R13944 T17583 T17591 arg1Of noted,","
R13945 T17583 T17592 arg1Of noted,respectively
R13946 T17586 T17584 arg2Of figure,in
R13947 T17586 T17585 arg1Of figure,the
R13948 T17588 T17589 arg1Of pMIP,and
R13949 T17589 T17587 arg2Of and,as
R1395 T1777 T1776 arg1Of ligand,the
R13950 T17590 T17589 arg2Of IKKγ,and
R13951 T17593 T17582 arg3Of ),(
R13952 T17594 T17595 arg1Of Lysates,(
R13953 T17594 T17599 arg1Of Lysates,and
R13954 T17597 T17595 arg2Of 1–3,(
R13955 T17597 T17596 arg1Of 1–3,lanes
R13956 T17598 T17595 arg3Of ),(
R13957 T17599 T17609 arg1Of and,from
R13958 T17599 T17613 arg1Of and,were
R13959 T17599 T17614 arg2Of and,fractionated
R1396 T1777 T1778 arg1Of ligand,for
R13960 T17599 T17618 arg2Of and,evaluated
R13961 T17602 T17601 arg2Of IP,(
R13962 T17603 T17601 arg3Of ),(
R13963 T17604 T17599 arg2Of samples,and
R13964 T17604 T17600 arg1Of samples,immunoprecipitation
R13965 T17604 T17601 arg1Of samples,(
R13966 T17604 T17605 arg1Of samples,(
R13967 T17606 T17605 arg2Of lanes,(
R13968 T17606 T17607 arg1Of lanes,4–11
R13969 T17608 T17605 arg3Of ),(
R1397 T1777 T1783 arg1Of ligand,is
R13970 T17612 T17609 arg2Of lines,from
R13971 T17612 T17610 arg1Of lines,the
R13972 T17612 T17611 arg1Of lines,cell
R13973 T17614 T17615 arg1Of fractionated,by
R13974 T17614 T17617 arg1Of fractionated,and
R13975 T17616 T17615 arg2Of SDS-PAGE,by
R13976 T17617 T17613 arg2Of and,were
R13977 T17617 T17622 arg1Of and,with
R13978 T17618 T17617 arg2Of evaluated,and
R13979 T17621 T17614 arg1Of blotting,fractionated
R1398 T1777 T1784 arg2Of ligand,expressed
R13980 T17621 T17618 arg1Of blotting,evaluated
R13981 T17621 T17619 arg2Of blotting,by
R13982 T17621 T17620 arg1Of blotting,Western
R13983 T17623 T17622 arg2Of antibodies,with
R13984 T17623 T17624 arg1Of antibodies,to
R13985 T17627 T17626 arg1Of tag,BP
R13986 T17627 T17628 arg1Of tag,(
R13987 T17627 T17634 arg1Of tag,and
R13988 T17629 T17628 arg2Of IKKγ,(
R13989 T17629 T17630 arg1Of IKKγ,","
R1399 T1779 T1778 arg2Of CD40,for
R13990 T17632 T17630 arg2Of panel,","
R13991 T17632 T17631 arg1Of panel,upper
R13992 T17633 T17628 arg3Of ),(
R13993 T17634 T17624 arg2Of and,to
R13994 T17634 T17625 arg1Of and,the
R13995 T17635 T17634 arg2Of TRAF2,and
R13996 T17639 T17636 arg1Of sample,The
R13997 T17639 T17637 arg1Of sample,anti-CD40
R13998 T17639 T17638 arg1Of sample,IP
R13999 T17639 T17640 arg1Of sample,in
R1400 T1779 T1780 arg1Of CD40,","
R14000 T17639 T17643 arg1Of sample,was
R14001 T17639 T17644 arg2Of sample,treated
R14002 T17641 T17640 arg2Of lane,in
R14003 T17641 T17642 arg1Of lane,11
R14004 T17644 T17643 arg2Of treated,was
R14005 T17644 T17645 arg1Of treated,with
R14006 T17644 T17648 arg1Of treated,;
R14007 T17647 T17645 arg2Of phosphatase,with
R14008 T17647 T17646 arg1Of phosphatase,λ
R14009 T17650 T17649 arg1Of sample,the
R1401 T1781 T1780 arg2Of CD154,","
R14010 T17650 T17651 arg1Of sample,in
R14011 T17650 T17654 arg1Of sample,was
R14012 T17650 T17655 arg1Of sample,mock-treated
R14013 T17652 T17651 arg2Of lane,in
R14014 T17652 T17653 arg1Of lane,10
R14015 T17654 T17648 arg2Of was,;
R14016 T17655 T17654 arg2Of mock-treated,was
R14017 T17657 T17656 arg1Of samples,Protein
R14018 T17657 T17665 arg1Of samples,were
R14019 T17657 T17667 arg2Of samples,fractionated
R1402 T1784 T1775 arg2Of expressed,as
R14020 T17660 T17661 arg1Of those,treated
R14021 T17661 T17658 arg2Of treated,(
R14022 T17661 T17659 arg1Of treated,minus
R14023 T17661 T17662 arg1Of treated,with
R14024 T17663 T17662 arg2Of phosphatase,with
R14025 T17664 T17658 arg3Of ),(
R14026 T17667 T17658 arg1Of fractionated,(
R14027 T17667 T17665 arg2Of fractionated,were
R14028 T17667 T17666 arg1Of fractionated,also
R14029 T17667 T17668 arg1Of fractionated,on
R1403 T1784 T1782 arg1Of expressed,","
R14030 T17667 T17677 arg1Of fractionated,for
R14031 T17671 T17668 arg2Of gel,on
R14032 T17671 T17669 arg1Of gel,a
R14033 T17671 T17670 arg1Of gel,separate
R14034 T17671 T17672 arg1Of gel,(
R14035 T17675 T17672 arg2Of concentration,(
R14036 T17675 T17673 arg1Of concentration,lower
R14037 T17675 T17674 arg1Of concentration,acrylamide
R14038 T17676 T17672 arg3Of ),(
R14039 T17679 T17677 arg2Of evaluation,for
R1404 T1784 T1783 arg2Of expressed,is
R14040 T17679 T17678 arg1Of evaluation,the
R14041 T17679 T17680 arg1Of evaluation,of
R14042 T17681 T17682 arg1Of TRAF3,and
R14043 T17682 T17680 arg2Of and,of
R14044 T17682 T17684 arg1Of and,(
R14045 T17683 T17682 arg2Of HOIP,and
R14046 T17687 T17684 arg2Of panels,(
R14047 T17687 T17685 arg1Of panels,bottom
R14048 T17687 T17686 arg1Of panels,two
R14049 T17688 T17684 arg3Of ),(
R1405 T1784 T1785 arg1Of expressed,primarily
R14050 T17690 T17689 arg1Of results,Similar
R14051 T17690 T17691 arg1Of results,were
R14052 T17690 T17692 arg2Of results,obtained
R14053 T17692 T17691 arg2Of obtained,were
R14054 T17692 T17693 arg1Of obtained,in
R14055 T17696 T17693 arg2Of experiments,in
R14056 T17696 T17694 arg1Of experiments,two
R14057 T17696 T17695 arg1Of experiments,additional
R1406 T1789 T1784 arg1Of cells,expressed
R1407 T1789 T1786 arg2Of cells,by
R1408 T1789 T1787 arg2Of cells,activated
R1409 T1789 T1788 arg1Of cells,T
R1410 T1791 T1790 arg1Of engagement,CD40
R1411 T1791 T1792 arg1Of engagement,leads
R1412 T1792 T1793 arg1Of leads,to
R1413 T1795 T1793 arg2Of activation,to
R1414 T1795 T1794 arg1Of activation,the
R1415 T1795 T1796 arg1Of activation,of
R1416 T1799 T1796 arg2Of molecules,of
R1417 T1799 T1797 arg1Of molecules,various
R1418 T1799 T1798 arg1Of molecules,signaling
R1419 T1799 T1800 arg1Of molecules,","
R1420 T1799 T1801 arg1Of molecules,including
R1421 T1804 T1802 arg1Of kinases,stress-activated
R1422 T1804 T1803 arg1Of kinases,protein
R1423 T1804 T1805 arg1Of kinases,and
R1424 T1805 T1801 arg2Of and,including
R1425 T1809 T1805 arg2Of NF-κB,and
R1426 T1809 T1806 arg1Of NF-κB,the
R1427 T1809 T1807 arg1Of NF-κB,transcription
R1428 T1809 T1808 arg1Of NF-κB,factor
R1429 T1809 T1810 arg1Of NF-κB,","
R1430 T1809 T1811 arg1Of NF-κB,which
R1431 T1809 T1812 arg1Of NF-κB,upregulate
R1432 T1814 T1812 arg2Of expression,upregulate
R1433 T1814 T1813 arg1Of expression,the
R1434 T1814 T1815 arg1Of expression,of
R1435 T1816 T1817 arg1Of cytokines,and
R1436 T1817 T1815 arg2Of and,of
R1437 T1817 T1820 arg1Of and,that
R1438 T1817 T1821 arg1Of and,promote
R1439 T1819 T1817 arg2Of factors,and
R1440 T1819 T1818 arg1Of factors,other
R1441 T1823 T1821 arg2Of responses,promote
R1442 T1823 T1822 arg1Of responses,immune
R1443 T1825 T1824 arg1Of mechanism,The
R1444 T1825 T1826 arg2Of mechanism,by
R1445 T1825 T1827 arg1Of mechanism,which
R1446 T1825 T1833 arg1Of mechanism,has
R1447 T1825 T1835 arg1Of mechanism,been
R1448 T1825 T1837 arg2Of mechanism,defined
R1449 T1828 T1829 arg1Of CD40,induces
R1450 T1829 T1826 arg1Of induces,by
R1451 T1832 T1829 arg2Of pathways,induces
R1452 T1832 T1830 arg1Of pathways,these
R1453 T1832 T1831 arg1Of pathways,signaling
R1454 T1837 T1833 arg2Of defined,has
R1455 T1837 T1834 arg1Of defined,not
R1456 T1837 T1835 arg2Of defined,been
R1457 T1837 T1836 arg1Of defined,completely
R1458 T1840 T1838 arg1Of domain,The
R1459 T1840 T1839 arg1Of domain,cytoplasmic
R1460 T1840 T1841 arg1Of domain,of
R1461 T1840 T1843 arg1Of domain,does
R1462 T1840 T1845 arg1Of domain,appear
R1463 T1840 T1847 arg1Of domain,have
R1464 T1840 T1853 arg1Of domain,is
R1465 T1840 T1854 arg1Of domain,able
R1466 T1840 T1856 arg1Of domain,mediate
R1467 T1842 T1841 arg2Of CD40,of
R1468 T1845 T1843 arg2Of appear,does
R1469 T1845 T1844 arg1Of appear,not
R1470 T1845 T1852 arg1Of appear,but
R1471 T1847 T1845 arg2Of have,appear
R1472 T1847 T1846 arg1Of have,to
R1473 T1850 T1847 arg2Of activity,have
R1474 T1850 T1848 arg1Of activity,intrinsic
R1475 T1850 T1849 arg1Of activity,enzymatic
R1476 T1852 T1851 arg1Of but,","
R1477 T1853 T1852 arg2Of is,but
R1478 T1854 T1853 arg2Of able,is
R1479 T1856 T1854 arg2Of mediate,able
R1480 T1856 T1855 arg1Of mediate,to
R1481 T1856 T1858 arg1Of mediate,through
R1482 T1857 T1856 arg2Of signaling,mediate
R1483 T1860 T1858 arg2Of recruitment,through
R1484 T1860 T1859 arg1Of recruitment,the
R1485 T1860 T1861 arg1Of recruitment,of
R1486 T1864 T1861 arg2Of proteins,of
R1487 T1864 T1862 arg1Of proteins,several
R1488 T1864 T1863 arg1Of proteins,intracellular
R1489 T1865 T1866 arg1Of Members,of
R1490 T1865 T1875 arg1Of Members,","
R1491 T1865 T1876 arg1Of Members,including
R1492 T1865 T1886 arg1Of Members,appear
R1493 T1865 T1888 arg1Of Members,be
R1494 T1900 T1899 arg2Of 4,[
R1495 T1865 T1890 arg1Of Members,important
R1496 T1872 T1871 arg2Of TRAF,(
R1497 T1901 T1899 arg3Of ],[
R1498 T1903 T1902 arg1Of proteins,These
R1499 T1903 T1904 arg1Of proteins,function
R1500 T1903 T1911 arg1Of proteins,binding
R1501 T1903 T1919 arg1Of proteins,recruiting
R1502 T1904 T1905 arg1Of function,in
R1503 T1904 T1907 arg1Of function,as
R1504 T1904 T1910 arg1Of function,","
R1505 T1904 T1911 modOf function,binding
R1506 T1904 T1919 modOf function,recruiting
R1507 T1906 T1905 arg2Of part,in
R1508 T1909 T1907 arg2Of molecules,as
R1509 T1909 T1908 arg1Of molecules,adaptor
R1510 T1911 T1912 arg1Of binding,to
R1511 T1911 T1918 arg1Of binding,and
R1512 T1873 T1871 arg3Of ),(
R1513 T1874 T1866 arg2Of family,of
R1514 T1915 T1912 arg2Of tail,to
R1515 T1915 T1913 arg1Of tail,the
R1516 T1915 T1914 arg1Of tail,cytoplasmic
R1517 T1915 T1916 arg1Of tail,of
R1518 T1917 T1916 arg2Of CD40,of
R1519 T1919 T1918 arg2Of recruiting,and
R1520 T1874 T1867 arg1Of family,the
R1521 T1921 T1919 arg2Of proteins,recruiting
R1522 T1921 T1920 arg1Of proteins,other
R1523 T1921 T1922 arg1Of proteins,to
R1524 T1874 T1868 arg1Of family,TNF
R1525 T1874 T1869 arg1Of family,receptor-associated
R1526 T1874 T1870 arg1Of family,factor
R1527 T1925 T1922 arg2Of complex,to
R1528 T1925 T1923 arg1Of complex,the
R1529 T1925 T1924 arg1Of complex,receptor-associated
R1530 T1874 T1871 arg1Of family,(
R1531 T1877 T1878 arg1Of TRAF1,","
R1532 T1926 T1927 arg1Of Some,of
R1533 T1926 T1931 arg1Of Some,function
R1534 T1929 T1927 arg2Of TRAFs,of
R1535 T1929 T1928 arg1Of TRAFs,the
R1536 T1931 T1930 arg1Of function,also
R1537 T1931 T1932 arg1Of function,as
R1538 T1931 T1937 arg1Of function,and
R1539 T1878 T1880 arg1Of ",",","
R1540 T1935 T1932 arg2Of ligases,as
R1541 T1935 T1933 arg1Of ligases,E3
R1542 T1935 T1934 arg1Of ligases,ubiquitin
R1543 T1937 T1936 arg1Of and,","
R1544 T1879 T1878 arg2Of TRAF2,","
R1545 T1880 T1883 arg1Of ",",and
R1546 T1881 T1880 arg2Of TRAF3,","
R1547 T1940 T1938 arg1Of activity,this
R1548 T1940 T1939 arg1Of activity,enzymatic
R1549 T1940 T1941 arg1Of activity,may
R1550 T1940 T1942 arg1Of activity,contribute
R1551 T1883 T1876 arg2Of and,including
R1552 T1942 T1937 arg2Of contribute,and
R1553 T1942 T1941 arg2Of contribute,may
R1554 T1942 T1943 arg1Of contribute,to
R1555 T1945 T1946 arg1Of propagation,and
R1556 T1883 T1882 arg1Of and,","
R1557 T1946 T1943 arg2Of and,to
R1558 T1946 T1944 arg1Of and,signal
R1559 T1947 T1946 arg2Of regulation,and
R1560 T1884 T1883 arg2Of TRAF6,and
R1561 T1886 T1885 arg1Of appear,","
R1562 T1951 T1948 arg2Of TRAFs,Among
R1563 T1951 T1949 arg1Of TRAFs,the
R1564 T1951 T1950 arg1Of TRAFs,multiple
R1565 T1951 T1952 arg1Of TRAFs,that
R1566 T1951 T1953 arg1Of TRAFs,associate
R1567 T1953 T1954 arg1Of associate,with
R1568 T1955 T1954 arg2Of CD40,with
R1569 T1957 T1958 arg1Of TRAF3,can
R1570 T1957 T1959 arg1Of TRAF3,function
R1571 T1886 T1899 arg1Of appear,[
R1572 T1959 T1948 arg1Of function,Among
R1573 T1959 T1956 arg1Of function,","
R1574 T1959 T1958 arg2Of function,can
R1575 T1959 T1960 arg1Of function,as
R1576 T1959 T1966 arg1Of function,","
R1577 T1959 T1967 arg1Of function,while
R1578 T1888 T1886 arg2Of be,appear
R1579 T1963 T1960 arg2Of regulator,as
R1580 T1963 T1961 arg1Of regulator,a
R1581 T1963 T1962 arg1Of regulator,negative
R1582 T1963 T1964 arg1Of regulator,of
R1583 T1965 T1964 arg2Of signaling,of
R1584 T1888 T1887 arg1Of be,to
R1585 T1890 T1888 arg2Of important,be
R1586 T1968 T1969 arg1Of TRAF2,and
R1587 T1969 T1971 arg1Of and,promote
R1588 T1890 T1889 arg1Of important,particularly
R1589 T1970 T1969 arg2Of TRAF6,and
R1590 T1971 T1967 arg2Of promote,while
R1591 T1890 T1891 arg1Of important,for
R1592 T1893 T1894 arg1Of initiation,and
R1593 T1973 T1971 arg2Of activation,promote
R1594 T1973 T1972 arg1Of activation,the
R1595 T1973 T1974 arg1Of activation,of
R1596 T1977 T1974 arg2Of pathways,of
R1597 T1977 T1975 arg1Of pathways,downstream
R1598 T1977 T1976 arg1Of pathways,signaling
R1599 T1977 T1978 arg1Of pathways,[
R1600 T1894 T1891 arg2Of and,for
R1601 T1979 T1978 arg2Of 4,[
R1602 T1980 T1978 arg3Of ],[
R1603 T1981 T1983 arg1Of We,demonstrated
R1604 T1894 T1892 arg1Of and,the
R1605 T1894 T1896 arg1Of and,of
R1606 T1983 T1982 arg1Of demonstrated,recently
R1607 T1895 T1894 arg2Of regulation,and
R1608 T1898 T1896 arg2Of signaling,of
R1609 T1987 T1985 arg1Of protein,HOIL-1L
R1610 T1987 T1986 arg1Of protein,interacting
R1611 T1987 T1988 arg1Of protein,(
R1612 T1987 T1991 arg1Of protein,","
R1613 T1987 T2008 arg1Of protein,is
R1614 T1987 T2009 arg2Of protein,recruited
R1615 T1989 T1988 arg2Of HOIP,(
R1616 T1990 T1988 arg3Of ),(
R1617 T1994 T1991 arg2Of ligase,","
R1618 T1994 T1992 arg1Of ligase,a
R1619 T1994 T1993 arg1Of ligase,ubiquitin
R1620 T1994 T1995 arg1Of ligase,that
R1621 T1994 T1996 arg1Of ligase,can
R1622 T1994 T1997 arg1Of ligase,catalyze
R1623 T1898 T1897 arg1Of signaling,CD40
R1624 T2094 T2090 arg2Of signaling,for
R1625 T2094 T2091 arg1Of signaling,B
R1626 T1997 T1996 arg2Of catalyze,can
R1627 T2094 T2092 arg1Of signaling,cell
R1628 T2094 T2093 arg1Of signaling,CD40
R1629 T1999 T1997 arg2Of assembly,catalyze
R1630 T2094 T2095 arg1Of signaling,[
R1631 T1999 T1998 arg1Of assembly,the
R1632 T1999 T2000 arg1Of assembly,of
R1633 T2096 T2095 arg2Of 7,[
R1634 T2003 T2000 arg2Of chains,of
R1635 T2003 T2001 arg1Of chains,linear
R1636 T2003 T2002 arg1Of chains,polyubiquitin
R1637 T2003 T2004 arg1Of chains,[
R1638 T2005 T2004 arg2Of 5,[
R1639 T2097 T2095 arg3Of ],[
R1640 T2006 T2004 arg3Of ],[
R1641 T2100 T2070 arg2Of 8,targeting
R1642 T2009 T1983 arg2Of recruited,demonstrated
R1643 T2009 T1984 arg1Of recruited,that
R1644 T2009 T2007 arg1Of recruited,","
R1645 T2009 T2008 arg2Of recruited,is
R1646 T2009 T2010 arg1Of recruited,to
R1647 T2009 T2012 arg1Of recruited,in
R1648 T2009 T2016 arg1Of recruited,following
R1649 T2011 T2010 arg2Of CD40,to
R1650 T2100 T2099 arg2Of 8,[
R1651 T2101 T2099 arg3Of ],[
R1652 T2015 T2012 arg2Of manner,in
R1653 T2015 T2013 arg1Of manner,a
R1654 T2015 T2014 arg1Of manner,TRAF2-dependent
R1655 T2017 T2016 arg2Of engagement,following
R1656 T2017 T2018 arg1Of engagement,of
R1657 T2017 T2020 arg1Of engagement,by
R1658 T2017 T2023 arg1Of engagement,[
R1659 T2104 T2103 arg2Of 9,[
R1660 T2019 T2018 arg2Of CD40,of
R1661 T2022 T2020 arg2Of antibody,by
R1662 T2022 T2021 arg1Of antibody,agonistic
R1663 T2105 T2103 arg3Of ],[
R1664 T2024 T2023 arg2Of 6,[
R1665 T2025 T2023 arg3Of ],[
R1666 T2029 T2026 arg1Of findings,These
R1667 T2029 T2027 arg1Of findings,and
R1668 T2029 T2028 arg1Of findings,other
R1669 T2029 T2030 arg1Of findings,led
R1670 T2106 T2107 arg1Of We,found
R1671 T2031 T2030 arg2Of us,led
R1672 T2031 T2033 arg1Of us,hypothesize
R1673 T2033 T2030 arg3Of hypothesize,led
R1674 T2033 T2032 arg1Of hypothesize,to
R1675 T2111 T2109 arg1Of upregulation,the
R1676 T2035 T2036 arg1Of HOIP,functions
R1677 T2036 T2034 arg1Of functions,that
R1678 T2036 T2037 arg1Of functions,downstream
R1679 T2036 T2040 arg1Of functions,in
R1680 T2036 T2045 arg1Of functions,and
R1681 T2037 T2038 arg1Of downstream,of
R1682 T2039 T2038 arg2Of TRAF2,of
R1683 T2111 T2110 arg1Of upregulation,CD40-induced
R1684 T2044 T2040 arg2Of pathway,in
R1685 T2044 T2041 arg1Of pathway,the
R1686 T2044 T2042 arg1Of pathway,CD40
R1687 T2044 T2043 arg1Of pathway,signaling
R1688 T2045 T2033 arg2Of and,hypothesize
R1689 T2111 T2112 arg1Of upregulation,of
R1690 T2046 T2045 arg2Of that,and
R1691 T2111 T2122 arg1Of upregulation,was
R1692 T2047 T2048 arg1Of HOIP,is
R1693 T2047 T2049 arg1Of HOIP,necessary
R1694 T2048 T2046 arg2Of is,that
R1695 T2111 T2123 arg1Of upregulation,defective
R1696 T2049 T2048 arg2Of necessary,is
R1697 T2049 T2050 arg1Of necessary,for
R1698 T2113 T2112 arg2Of CD80,of
R1699 T2052 T2050 arg2Of activation,for
R1700 T2052 T2051 arg1Of activation,the
R1701 T2052 T2053 arg1Of activation,of
R1702 T2054 T2055 arg1Of NF-κB,and
R1703 T2055 T2053 arg2Of and,of
R1704 T2113 T2114 arg1Of CD80,(
R1705 T2117 T2114 arg2Of molecule,(
R1706 T2059 T2055 arg2Of molecules,and
R1707 T2059 T2056 arg1Of molecules,possibly
R1708 T2059 T2057 arg1Of molecules,other
R1709 T2059 T2058 arg1Of molecules,signaling
R1710 T2061 T2060 arg1Of test,To
R1711 T2063 T2061 arg2Of hypothesis,test
R1712 T2063 T2062 arg1Of hypothesis,this
R1713 T2117 T2115 arg1Of molecule,a
R1714 T2065 T2061 arg1Of we,test
R1715 T2065 T2066 arg1Of we,employed
R1716 T2066 T2060 modOf employed,To
R1717 T2066 T2064 arg1Of employed,","
R1718 T2117 T2116 arg1Of molecule,costimulatory
R1719 T2117 T2118 arg1Of molecule,for
R1720 T2066 T2102 arg1Of employed,","
R1721 T2066 T2103 arg1Of employed,[
R1722 T2120 T2118 arg2Of cells,for
R1723 T2069 T2066 arg2Of gene,employed
R1724 T2069 T2067 arg1Of gene,somatic
R1725 T2069 T2068 arg1Of gene,cell
R1726 T2069 T2070 arg1Of gene,targeting
R1727 T2070 T2071 modOf targeting,to
R1728 T2070 T2098 arg1Of targeting,","
R1729 T2072 T2071 arg1Of ablate,to
R1730 T2073 T2072 arg2Of expression,ablate
R1731 T2073 T2074 arg1Of expression,of
R1732 T2073 T2076 arg1Of expression,in
R1733 T2120 T2119 arg1Of cells,T
R1734 T2121 T2114 arg3Of ),(
R1735 T2075 T2074 arg2Of HOIP,of
R1736 T2122 T2107 arg2Of was,found
R1737 T2122 T2108 arg1Of was,that
R1738 T2122 T2124 arg1Of was,in
R1739 T2081 T2076 arg2Of line,in
R1740 T2081 T2077 arg1Of line,a
R1741 T2081 T2078 arg1Of line,mouse
R1742 T2081 T2079 arg1Of line,B
R1743 T2123 T2122 arg2Of defective,was
R1744 T2081 T2080 arg1Of line,cell
R1745 T2081 T2082 arg1Of line,that
R1746 T2081 T2083 arg1Of line,has
R1747 T2081 T2084 arg1Of line,proven
R1748 T2081 T2086 arg1Of line,be
R1749 T2126 T2124 arg2Of cells,in
R1750 T2126 T2125 arg1Of cells,HOIP-deficient
R1751 T2084 T2083 arg2Of proven,has
R1752 T2130 T2131 arg1Of CD40,and
R1753 T2086 T2084 arg2Of be,proven
R1754 T2086 T2085 arg1Of be,to
R1755 T2089 T2086 arg2Of model,be
R1756 T2089 T2087 arg1Of model,a
R1757 T2089 T2088 arg1Of model,useful
R1758 T2089 T2090 arg1Of model,for
R1759 T2132 T2131 arg2Of IL-4,and
R1760 T2134 T2129 arg1Of production,the
R1761 T2134 T2130 arg1Of production,CD40
R1762 T2134 T2132 arg1Of production,IL-4
R1763 T2134 T2133 arg1Of production,driven
R1764 T2192 T2189 arg2Of activation,with
R1765 T2192 T2190 arg1Of activation,impaired
R1766 T2192 T2191 arg1Of activation,NF-κB
R1767 T2194 T2188 arg1Of association,Consistent
R1768 T2194 T2195 arg1Of association,of
R1769 T2194 T2203 arg1Of association,with
R1770 T2194 T2205 arg1Of association,was
R1771 T2194 T2206 arg1Of association,undetectable
R1772 T2134 T2135 arg1Of production,of
R1773 T2134 T2157 arg1Of production,was
R1774 T2198 T2197 arg1Of kinase,IκB
R1775 T2198 T2199 arg1Of kinase,(
R1776 T2134 T2158 arg1Of production,defective
R1777 T2200 T2199 arg2Of IKK,(
R1778 T2137 T2135 arg2Of transcripts,of
R1779 T2201 T2199 arg3Of ),(
R1780 T2202 T2195 arg2Of complex,of
R1781 T2202 T2196 arg1Of complex,the
R1782 T2202 T2198 arg1Of complex,kinase
R1783 T2204 T2203 arg2Of CD40,with
R1784 T2205 T2188 modOf was,Consistent
R1785 T2205 T2193 arg1Of was,","
R1786 T2205 T2207 arg1Of was,in
R1787 T2206 T2205 arg2Of undetectable,was
R1788 T2137 T2136 arg1Of transcripts,germline
R1789 T2137 T2138 arg1Of transcripts,from
R1790 T2209 T2207 arg2Of cells,in
R1791 T2209 T2208 arg1Of cells,HOIP-deficient
R1792 T2144 T2138 arg2Of locus,from
R1793 T2213 T2212 arg1Of results,our
R1794 T2213 T2214 arg1Of results,indicate
R1795 T2214 T2210 arg1Of indicate,Together
R1796 T2214 T2211 arg1Of indicate,","
R1797 T2216 T2217 arg1Of HOIP,plays
R1798 T2217 T2214 arg2Of plays,indicate
R1799 T2217 T2215 arg1Of plays,that
R1800 T2217 T2221 arg1Of plays,in
R1801 T2220 T2217 arg2Of role,plays
R1802 T2220 T2218 arg1Of role,a
R1803 T2220 T2219 arg1Of role,critical
R1804 T2144 T2139 arg1Of locus,the
R1805 T2223 T2221 arg2Of activation,in
R1806 T2223 T2222 arg1Of activation,the
R1807 T2223 T2224 arg1Of activation,of
R1808 T2144 T2140 arg1Of locus,immunoglobulin
R1809 T2144 T2141 arg1Of locus,epsilon
R1810 T2144 T2142 arg1Of locus,heavy
R1811 T2226 T2224 arg2Of pathways,of
R1812 T2226 T2225 arg1Of pathways,signaling
R1813 T2226 T2227 arg1Of pathways,that
R1814 T2226 T2228 arg1Of pathways,regulate
R1815 T2144 T2143 arg1Of locus,chain
R1816 T2144 T2145 arg1Of locus,","
R1817 T2230 T2228 arg2Of responses,regulate
R1818 T2230 T2229 arg1Of responses,cellular
R1819 T2230 T2231 arg1Of responses,to
R1820 T2147 T2145 arg2Of event,","
R1821 T2233 T2231 arg2Of engagement,to
R1822 T2233 T2232 arg1Of engagement,CD40
R1823 T2147 T2146 arg1Of event,an
R1824 T2147 T2148 arg1Of event,that
R1825 T2147 T2149 arg1Of event,precedes
R1826 T2152 T2150 arg1Of rearrangement,immunoglobulin
R1827 T2152 T2151 arg1Of rearrangement,gene
R1828 T2152 T2153 arg1Of rearrangement,and
R1829 T2153 T2149 arg2Of and,precedes
R1834 T2155 T2153 arg2Of switching,and
R1835 T2155 T2154 arg1Of switching,isotype
R1839 T2157 T2127 arg1Of was,Similarly
R1841 T2157 T2128 arg1Of was,","
R1842 T2157 T2156 arg1Of was,","
R1843 T2157 T2159 arg1Of was,in
R1844 T2158 T2157 arg2Of defective,was
R1845 T2161 T2159 arg2Of absence,in
R1846 T2161 T2160 arg1Of absence,the
R1847 T2161 T2162 arg1Of absence,of
R1848 T2163 T2162 arg2Of HOIP,of
R1849 T2164 T2166 arg1Of We,found
R1850 T2166 T2165 arg1Of found,also
R1851 T2170 T2168 arg1Of activation,the
R1852 T2170 T2169 arg1Of activation,CD40-mediated
R1853 T2170 T2171 arg1Of activation,of
R1854 T2170 T2173 arg1Of activation,and
R1855 T2172 T2171 arg2Of NF-κB,of
R1856 T2173 T2183 arg1Of and,was
R1857 T2173 T2184 arg1Of and,defective
R1858 T2179 T2173 arg2Of kinase,and
R1859 T2179 T2174 arg1Of kinase,the
R1860 T2179 T2175 arg1Of kinase,stress-activated
R1861 T2179 T2176 arg1Of kinase,protein
R1862 T2179 T2177 arg1Of kinase,kinase
R1863 T2179 T2178 arg1Of kinase,c-Jun
R1864 T2179 T2180 arg1Of kinase,(
R1865 T2181 T2180 arg2Of JNK,(
R1866 T2182 T2180 arg3Of ),(
R1867 T2183 T2166 arg2Of was,found
R1868 T2183 T2167 arg1Of was,that
R1869 T2183 T2185 arg1Of was,in
R1870 T2184 T2183 arg2Of defective,was
R1871 T2187 T2185 arg2Of cells,in
R1872 T2187 T2186 arg1Of cells,HOIP-deficient
R1873 T2188 T2189 arg1Of Consistent,with
R2586 T3341 T3342 arg1Of Generation,of
R2587 T3341 T3346 arg1Of Generation,via
R2588 T3345 T3342 arg2Of cells,of
R2589 T3345 T3343 arg1Of cells,HOIP-deficient
R2590 T3345 T3344 arg1Of cells,B
R2591 T3348 T3346 arg2Of disruption,via
R2592 T3348 T3347 arg2Of disruption,targeted
R2593 T3348 T3349 arg1Of disruption,of
R2594 T3350 T3349 arg2Of Rnf31,of
R2595 T3350 T3351 arg1Of Rnf31,","
R2596 T3353 T3351 arg2Of gene,","
R2597 T3353 T3352 arg1Of gene,the
R2598 T3353 T3354 arg1Of gene,encoding
R2599 T3355 T3354 arg2Of HOIP,encoding
R2600 T3359 T3356 arg2Of study,In
R2601 T3359 T3357 arg1Of study,our
R2602 T3359 T3358 arg1Of study,previous
R2603 T3361 T3362 arg1Of we,demonstrated
R2604 T3362 T3356 arg1Of demonstrated,In
R2605 T3362 T3360 arg1Of demonstrated,","
R2606 T3364 T3365 arg1Of HOIP,is
R2607 T3364 T3366 arg2Of HOIP,recruited
R2608 T3366 T3362 arg2Of recruited,demonstrated
R2609 T3366 T3363 arg1Of recruited,that
R2610 T3366 T3365 arg2Of recruited,is
R2611 T3366 T3367 arg1Of recruited,to
R2612 T3366 T3372 arg1Of recruited,in
R2613 T3366 T3378 arg1Of recruited,[
R2614 T3371 T3367 arg2Of complex,to
R2615 T3371 T3368 arg1Of complex,the
R2616 T3371 T3369 arg1Of complex,CD40
R2617 T3371 T3370 arg1Of complex,signaling
R2618 T3377 T3372 arg2Of lines,in
R2619 T3377 T3373 arg1Of lines,two
R2620 T3377 T3374 arg1Of lines,mouse
R2621 T3377 T3375 arg1Of lines,B
R2622 T3377 T3376 arg1Of lines,cell
R2623 T3379 T3378 arg2Of 6,[
R2624 T3380 T3378 arg3Of ],[
R2625 T3382 T3381 arg1Of results,Similar
R2626 T3382 T3383 arg1Of results,were
R2627 T3382 T3384 arg2Of results,obtained
R2628 T3384 T3383 arg2Of obtained,were
R2629 T3384 T3385 arg1Of obtained,with
R2630 T3384 T3388 arg1Of obtained,","
R2631 T3384 T3389 modOf obtained,indicating
R2632 T3387 T3385 arg2Of splenocytes,with
R2633 T3387 T3386 arg1Of splenocytes,mouse
R2634 T3392 T3391 arg1Of interaction,the
R2635 T3392 T3393 arg1Of interaction,of
R2636 T3392 T3395 arg1Of interaction,with
R2637 T3392 T3397 arg1Of interaction,is
R2638 T3392 T3399 arg2Of interaction,limited
R2639 T3394 T3393 arg2Of CD40,of
R2640 T3396 T3395 arg2Of HOIP,with
R2641 T3399 T3389 arg2Of limited,indicating
R2642 T3399 T3390 arg1Of limited,that
R2643 T3399 T3397 arg2Of limited,is
R2644 T3399 T3398 arg1Of limited,not
R2645 T3399 T3400 arg1Of limited,to
R2646 T3404 T3400 arg2Of lines,to
R2647 T3404 T3401 arg2Of lines,transformed
R2648 T3404 T3402 arg1Of lines,B
R2649 T3404 T3403 arg1Of lines,cell
R2650 T3404 T3405 arg1Of lines,(
R2651 T3407 T3405 arg2Of observations,(
R2652 T3407 T3406 arg1Of observations,unpublished
R2653 T3408 T3405 arg3Of ),(
R2654 T3409 T3411 arg1Of We,found
R2655 T3411 T3410 arg1Of found,also
R2656 T3414 T3413 arg1Of recruitment,the
R2657 T3414 T3415 arg1Of recruitment,of
R2658 T3414 T3417 arg1Of recruitment,to
R2659 T3414 T3419 arg1Of recruitment,was
R2660 T3414 T3420 arg1Of recruitment,TRAF2-dependent
R2661 T3416 T3415 arg2Of HOIP,of
R2662 T3418 T3417 arg2Of CD40,to
R2663 T3419 T3412 arg1Of was,that
R2664 T3419 T3421 arg1Of was,and
R2665 T3420 T3419 arg2Of TRAF2-dependent,was
R2666 T3421 T3411 arg2Of and,found
R2667 T3422 T3421 arg2Of that,and
R2668 T3423 T3424 arg1Of overexpression,of
R2669 T3423 T3430 arg1Of overexpression,inhibited
R2670 T3428 T3424 arg2Of mutant,of
R2671 T3428 T3425 arg1Of mutant,a
R2672 T3428 T3426 arg2Of mutant,truncated
R2673 T3428 T3427 arg1Of mutant,HOIP
R2674 T3430 T3422 arg2Of inhibited,that
R2675 T3430 T3429 arg1Of inhibited,partially
R2676 T3433 T3430 arg2Of activation,inhibited
R2677 T3433 T3431 arg1Of activation,CD40-mediated
R2678 T3433 T3432 arg1Of activation,NF-κB
R2679 T3435 T3434 arg1Of results,These
R2680 T3435 T3436 arg1Of results,support
R2681 T3438 T3436 arg2Of hypothesis,support
R2682 T3438 T3437 arg1Of hypothesis,the
R2683 T3440 T3441 arg1Of HOIP,plays
R2684 T3441 T3438 arg2Of plays,hypothesis
R2685 T3441 T3439 arg1Of plays,that
R2686 T3441 T3444 arg1Of plays,in
R2687 T3443 T3441 arg2Of role,plays
R2688 T3443 T3442 arg1Of role,a
R2689 T3447 T3444 arg2Of transduction,in
R2690 T3447 T3445 arg1Of transduction,CD40
R2691 T3447 T3446 arg1Of transduction,signal
R2692 T3450 T3453 arg1Of define,and
R2693 T3452 T3450 arg2Of role,define
R2694 T3452 T3451 arg1Of role,the
R2695 T3453 T3448 arg1Of and,To
R2696 T3453 T3449 arg1Of and,further
R2697 T3454 T3453 arg2Of evaluate,and
R2698 T3456 T3454 arg2Of importance,evaluate
R2699 T3456 T3455 arg1Of importance,the
R2700 T3456 T3457 arg1Of importance,of
R2701 T3456 T3459 arg1Of importance,in
R2702 T3458 T3457 arg2Of HOIP,of
R2703 T3461 T3459 arg2Of signaling,in
R2704 T3461 T3460 arg1Of signaling,CD40
R2705 T3463 T3450 arg1Of we,define
R2706 T3463 T3454 arg1Of we,evaluate
R2707 T3463 T3464 arg1Of we,used
R2708 T3464 T3448 modOf used,To
R2709 T3464 T3462 arg1Of used,","
R2710 T3467 T3464 arg2Of gene,used
R2711 T3467 T3465 arg1Of gene,somatic
R2712 T3467 T3466 arg1Of gene,cell
R2713 T3467 T3468 arg1Of gene,targeting
R2714 T3468 T3469 modOf targeting,to
R2715 T3470 T3469 arg1Of disrupt,to
R2716 T3472 T3470 arg2Of gene,disrupt
R2717 T3472 T3471 arg1Of gene,the
R2718 T3472 T3473 arg1Of gene,encoding
R2719 T3473 T3475 arg1Of encoding,in
R2720 T3474 T3473 arg2Of HOIP,encoding
R2721 T3480 T3475 arg2Of line,in
R2722 T3480 T3476 arg1Of line,the
R2723 T3480 T3477 arg1Of line,mouse
R2724 T3480 T3478 arg1Of line,B
R2725 T3480 T3479 arg1Of line,cell
R2726 T3481 T3468 arg2Of A20.2J,targeting
R2727 T3484 T3482 arg1Of line,This
R2728 T3484 T3483 arg1Of line,cell
R2729 T3484 T3485 arg1Of line,has
R2730 T3484 T3486 arg1Of line,been
R2731 T3484 T3488 arg1Of line,useful
R2732 T3486 T3485 arg2Of been,has
R2733 T3486 T3514 arg1Of been,","
R2734 T3486 T3515 arg1Of been,[
R2735 T3486 T3518 arg1Of been,","
R2736 T3486 T3519 arg1Of been,[
R2737 T3488 T3486 arg2Of useful,been
R2738 T3488 T3487 arg1Of useful,particularly
R2739 T3488 T3489 arg1Of useful,in
R2740 T3488 T3497 arg1Of useful,to
R2741 T3491 T3489 arg2Of characterization,in
R2742 T3491 T3490 arg1Of characterization,the
R2743 T3491 T3492 arg1Of characterization,of
R2744 T3495 T3492 arg2Of mechanisms,of
R2745 T3495 T3493 arg1Of mechanisms,CD40
R2746 T3495 T3494 arg1Of mechanisms,signaling
R2747 T3497 T3496 arg1Of to,due
R2748 T3500 T3497 arg2Of ease,to
R2749 T3500 T3498 arg1Of ease,the
R2750 T3500 T3499 arg1Of ease,relative
R2751 T3500 T3501 arg2Of ease,with
R2752 T3500 T3502 arg1Of ease,which
R2753 T3504 T3503 arg1Of genome,its
R2754 T3504 T3505 arg1Of genome,can
R2755 T3504 T3506 arg1Of genome,be
R2756 T3504 T3507 arg2Of genome,modified
R2757 T3507 T3501 arg1Of modified,with
R2758 T3507 T3505 arg2Of modified,can
R2759 T3507 T3506 arg2Of modified,be
R2760 T3510 T3507 arg1Of recombination,modified
R2761 T3510 T3508 arg2Of recombination,by
R2762 T3510 T3509 arg1Of recombination,homologous
R2763 T3510 T3511 arg1Of recombination,[
R2764 T3512 T3511 arg2Of 7,[
R2765 T3513 T3511 arg3Of ],[
R2766 T3516 T3515 arg2Of 8,[
R2767 T3517 T3515 arg3Of ],[
R2768 T3520 T3519 arg2Of 9,[
R2769 T3521 T3519 arg3Of ],[
R2770 T3522 T3523 arg1Of We,used
R2771 T3522 T3541 arg1Of We,disrupt
R2772 T3524 T3525 arg1Of a,targeting
R2773 T3525 T3523 arg2Of targeting,used
R2774 T3525 T3524 arg1Of targeting,a
R2775 T3526 T3525 arg2Of vector,targeting
R2776 T3526 T3527 arg1Of vector,capable
R2777 T3527 T3528 arg1Of capable,of
R2778 T3529 T3528 arg2Of undergoing,of
R2779 T3529 T3532 arg1Of undergoing,with
R2780 T3531 T3529 arg2Of recombination,undergoing
R2781 T3531 T3530 arg1Of recombination,homologous
R2782 T3533 T3532 arg2Of Rnf31,with
R2783 T3533 T3534 arg1Of Rnf31,(
R2784 T3536 T3534 arg2Of gene,(
R2785 T3536 T3535 arg1Of gene,the
R2786 T3536 T3537 arg1Of gene,encoding
R2787 T3538 T3537 arg2Of HOIP,encoding
R2788 T3539 T3534 arg3Of ),(
R2789 T3541 T3523 arg3Of disrupt,used
R2790 T3541 T3540 arg1Of disrupt,to
R2791 T3544 T3541 arg2Of sequence,disrupt
R2792 T3544 T3542 arg1Of sequence,the
R2793 T3544 T3543 arg1Of sequence,coding
R2794 T3544 T3545 arg1Of sequence,of
R2795 T3544 T3548 arg1Of sequence,in
R2796 T3547 T3545 arg2Of gene,of
R2797 T3547 T3546 arg1Of gene,the
R2798 T3549 T3548 arg2Of exon,in
R2799 T3549 T3550 arg1Of exon,5
R2800 T3549 T3551 arg1Of exon,(
R2801 T3553 T3551 arg2Of 1A,(
R2802 T3553 T3552 arg1Of 1A,Fig.
R2803 T3554 T3551 arg3Of ),(
R2804 T3556 T3555 arg2Of introduction,Following
R2805 T3556 T3557 arg1Of introduction,of
R2806 T3559 T3557 arg2Of vector,of
R2807 T3559 T3558 arg1Of vector,the
R2808 T3563 T3561 arg1Of clones,the
R2809 T3563 T3562 arg1Of clones,neomycin-resistant
R2810 T3563 T3564 arg1Of clones,that
R2811 T3563 T3565 arg1Of clones,arose
R2812 T3563 T3566 arg1Of clones,were
R2813 T3563 T3567 arg2Of clones,screened
R2814 T3567 T3555 arg1Of screened,Following
R2815 T3567 T3560 arg1Of screened,","
R2816 T3567 T3566 arg2Of screened,were
R2817 T3570 T3567 arg1Of amplification,screened
R2818 T3570 T3568 arg2Of amplification,by
R2819 T3570 T3569 arg1Of amplification,PCR
R2820 T3570 T3571 arg1Of amplification,of
R2821 T3570 T3574 modOf amplification,to
R2822 T3573 T3571 arg2Of DNA,of
R2823 T3573 T3572 arg1Of DNA,genomic
R2824 T3575 T3574 arg1Of identify,to
R2825 T3576 T3575 arg2Of cells,identify
R2826 T3576 T3577 arg1Of cells,containing
R2827 T3581 T3577 arg2Of allele,containing
R2828 T3581 T3578 arg1Of allele,a
R2829 T3581 T3579 arg2Of allele,disrupted
R2830 T3581 T3580 arg1Of allele,Rnf31
R2831 T3583 T3582 arg1Of remove,To
R2832 T3588 T3583 arg2Of cassette,remove
R2833 T3588 T3584 arg1Of cassette,the
R2834 T3588 T3585 arg1Of cassette,selectable
R2835 T3588 T3586 arg1Of cassette,marker
R2836 T3588 T3587 arg1Of cassette,gene
R2837 T3588 T3589 arg1Of cassette,from
R2838 T3593 T3589 arg2Of allele,from
R2839 T3593 T3590 arg1Of allele,the
R2840 T3593 T3591 arg2Of allele,disrupted
R2841 T3593 T3592 arg1Of allele,Rnf31
R2842 T3597 T3595 arg1Of lines,recombinant
R2843 T3597 T3596 arg1Of lines,cell
R2844 T3597 T3598 arg1Of lines,were
R2845 T3597 T3600 arg2Of lines,transfected
R2846 T3600 T3582 modOf transfected,To
R2847 T3600 T3594 arg1Of transfected,","
R2848 T3600 T3598 arg2Of transfected,were
R2849 T3600 T3599 arg1Of transfected,transiently
R2850 T3600 T3601 arg1Of transfected,with
R2851 T3603 T3601 arg2Of plasmid,with
R2852 T3603 T3602 arg1Of plasmid,a
R2853 T3603 T3604 arg1Of plasmid,that
R2854 T3603 T3605 arg1Of plasmid,encodes
R2855 T3607 T3605 arg2Of recombinase,encodes
R2856 T3607 T3606 arg1Of recombinase,Cre
R2857 T3609 T3608 arg1Of step,This
R2858 T3609 T3610 arg1Of step,allowed
R2859 T3610 T3622 arg1Of allowed,","
R2860 T3610 T3623 modOf allowed,generating
R2861 T3611 T3610 arg2Of us,allowed
R2862 T3611 T3613 arg1Of us,perform
R2863 T3613 T3610 arg3Of perform,allowed
R2864 T3613 T3612 arg1Of perform,to
R2865 T3616 T3613 arg2Of round,perform
R2866 T3616 T3614 arg1Of round,a
R2867 T3616 T3615 arg1Of round,second
R2868 T3616 T3617 arg1Of round,of
R2869 T3618 T3619 arg1Of targeting,and
R2870 T3619 T3617 arg2Of and,of
R2871 T3621 T3619 arg2Of selection,and
R2872 T3621 T3620 arg1Of selection,drug
R2873 T3624 T3623 arg2Of cells,generating
R2874 T3624 T3625 arg2Of cells,in
R2875 T3624 T3626 arg1Of cells,which
R2876 T3628 T3627 arg1Of copies,both
R2877 T3628 T3629 arg1Of copies,of
R2878 T3628 T3631 arg1Of copies,were
R2879 T3628 T3632 arg2Of copies,disrupted
R2880 T3630 T3629 arg2Of Rnf31,of
R2881 T3632 T3625 arg1Of disrupted,in
R2882 T3632 T3631 arg2Of disrupted,were
R2883 T3637 T3633 arg1Of lines,Two
R2884 T3637 T3634 arg1Of lines,independent
R2885 T3637 T3635 arg1Of lines,clonal
R2886 T3637 T3636 arg1Of lines,cell
R2887 T3637 T3638 arg1Of lines,were
R2888 T3637 T3639 arg2Of lines,chosen
R2889 T3639 T3638 arg2Of chosen,were
R2890 T3639 T3640 arg1Of chosen,for
R2891 T3642 T3640 arg2Of analysis,for
R2892 T3642 T3641 arg1Of analysis,further
R2893 T3645 T3643 arg1Of expression,HOIP
R2894 T3645 T3644 arg1Of expression,protein
R2895 T3645 T3646 arg1Of expression,was
R2896 T3645 T3647 arg1Of expression,undetectable
R2897 T3646 T3652 arg1Of was,","
R2898 T3646 T3653 arg1Of was,as
R2899 T3646 T3666 arg1Of was,","
R2900 T3646 T3667 modOf was,demonstrating
R2901 T3647 T3646 arg2Of undetectable,was
R2902 T3647 T3648 arg1Of undetectable,in
R2903 T3651 T3648 arg2Of lines,in
R2904 T3651 T3649 arg1Of lines,both
R2905 T3651 T3650 arg1Of lines,cell
R2906 T3654 T3653 arg2Of determined,as
R2907 T3658 T3654 arg1Of analysis,determined
R2908 T3658 T3655 arg2Of analysis,by
R2909 T3658 T3656 arg1Of analysis,Western
R2910 T3658 T3657 arg1Of analysis,blot
R2911 T3658 T3659 arg1Of analysis,of
R2912 T3661 T3659 arg2Of lysates,of
R2913 T3661 T3660 arg1Of lysates,cell
R2914 T3661 T3662 arg1Of lysates,(
R2915 T3664 T3662 arg2Of 1B,(
R2916 T3664 T3663 arg1Of 1B,Fig.
R2917 T3665 T3662 arg3Of ),(
R2918 T3669 T3670 arg1Of the,targeting
R2919 T3670 T3669 arg1Of targeting,the
R2920 T3670 T3672 arg1Of targeting,was
R2921 T3670 T3673 arg1Of targeting,successful
R2922 T3671 T3670 arg2Of process,targeting
R2923 T3672 T3667 arg2Of was,demonstrating
R2924 T3672 T3668 arg1Of was,that
R2925 T3673 T3672 arg2Of successful,was
R2926 T3676 T3674 arg2Of text,In
R2927 T3676 T3675 arg1Of text,the
R2928 T3676 T3677 arg1Of text,that
R2929 T3676 T3678 arg1Of text,follows
R2930 T3684 T3680 arg1Of lines,the
R2931 T3684 T3681 arg1Of lines,two
R2932 T3684 T3682 arg1Of lines,gene-targeted
R2933 T3684 T3683 arg1Of lines,cell
R2934 T3684 T3685 arg1Of lines,are
R2935 T3684 T3686 arg2Of lines,referred
R2936 T3686 T3674 arg1Of referred,In
R2937 T3686 T3679 arg1Of referred,","
R2938 T3686 T3685 arg2Of referred,are
R2939 T3686 T3687 arg1Of referred,to
R2940 T3686 T3688 arg1Of referred,as
R2941 T3690 T3688 arg2Of cells,as
R2942 T3690 T3689 arg1Of cells,HOIP-deficient
R2943 T3692 T3691 arg1Of enable,To
R2944 T3693 T3692 arg2Of us,enable
R2945 T3693 T3695 arg1Of us,confirm
R2946 T3695 T3692 arg3Of confirm,enable
R2947 T3695 T3694 arg1Of confirm,to
R2948 T3698 T3699 arg1Of signaling,or
R2949 T3700 T3699 arg2Of functional,or
R2950 T3701 T3697 arg1Of defects,any
R2951 T3701 T3698 arg1Of defects,signaling
R2952 T3701 T3700 arg1Of defects,functional
R2953 T3701 T3702 arg2Of defects,observed
R2954 T3701 T3706 arg1Of defects,were
R2955 T3701 T3707 arg1Of defects,due
R2956 T3702 T3703 arg1Of observed,in
R2957 T3705 T3703 arg2Of cells,in
R2958 T3705 T3704 arg1Of cells,HOIP-deficient
R2959 T3706 T3695 arg2Of were,confirm
R2960 T3706 T3696 arg1Of were,that
R2961 T3707 T3706 arg2Of due,were
R2962 T3707 T3708 arg1Of due,to
R2963 T3709 T3708 arg2Of disruption,to
R2964 T3709 T3710 arg1Of disruption,of
R2965 T3713 T3710 arg2Of gene,of
R2966 T3713 T3711 arg1Of gene,the
R2967 T3713 T3712 arg1Of gene,Rnf31
R2968 T3718 T3715 arg1Of lines,both
R2969 T3718 T3716 arg1Of lines,HOIP-deficient
R2970 T3718 T3717 arg1Of lines,cell
R2971 T3718 T3719 arg1Of lines,were
R2972 T3718 T3720 arg2Of lines,transduced
R2973 T3718 T3730 arg1Of lines,restoring
R2974 T3720 T3691 modOf transduced,To
R2975 T3720 T3714 arg1Of transduced,","
R2976 T3720 T3719 arg2Of transduced,were
R2977 T3720 T3721 arg1Of transduced,with
R2978 T3720 T3728 arg1Of transduced,","
R2979 T3720 T3729 arg1Of transduced,thus
R2980 T3720 T3730 modOf transduced,restoring
R2981 T3723 T3721 arg2Of retrovirus,with
R2982 T3723 T3722 arg1Of retrovirus,a
R2983 T3723 T3724 arg1Of retrovirus,encoding
R2984 T3727 T3724 arg2Of HOIP,encoding
R2985 T3727 T3725 arg1Of HOIP,FLAG-tagged
R2986 T3727 T3726 arg1Of HOIP,wild-type
R2987 T3733 T3730 arg2Of expression,restoring
R2988 T3733 T3731 arg1Of expression,HOIP
R2989 T3733 T3732 arg1Of expression,protein
R2990 T3733 T3734 arg1Of expression,(
R2991 T3736 T3734 arg2Of 1B,(
R2992 T3736 T3735 arg1Of 1B,Fig.
R2993 T3737 T3734 arg3Of ),(
R2994 T3739 T3738 arg1Of cells,These
R2995 T3739 T3740 arg1Of cells,are
R2996 T3739 T3741 arg2Of cells,referred
R2997 T3741 T3740 arg2Of referred,are
R2998 T3741 T3742 arg1Of referred,to
R2999 T3741 T3743 arg1Of referred,as
R3000 T3745 T3743 arg2Of cells,as
R3001 T3745 T3744 arg1Of cells,HOIP-reconstituted
R3002 T3748 T3746 arg2Of experiments,In
R3003 T3748 T3747 arg1Of experiments,the
R3004 T3748 T3749 arg1Of experiments,that
R3005 T3748 T3750 arg1Of experiments,follow
R3006 T3752 T3753 arg1Of responses,by
R3007 T3752 T3756 arg1Of responses,were
R3008 T3752 T3757 arg2Of responses,compared
R3009 T3755 T3753 arg2Of cells,by
R3010 T3755 T3754 arg1Of cells,these
R3011 T3757 T3746 arg1Of compared,In
R3012 T3757 T3751 arg1Of compared,","
R3013 T3757 T3756 arg2Of compared,were
R3014 T3757 T3758 arg1Of compared,to
R3015 T3759 T3758 arg2Of those,to
R3016 T3759 T3760 arg1Of those,of
R3017 T3762 T3761 arg1Of cells,A20.2J
R3018 T3762 T3763 arg1Of cells,and
R3019 T3763 T3760 arg2Of and,of
R3020 T3763 T3766 arg2Of and,transduced
R3021 T3765 T3763 arg2Of cells,and
R3022 T3765 T3764 arg1Of cells,HOIP-deficient
R3023 T3766 T3767 arg1Of transduced,with
R3024 T3770 T3767 arg2Of vector,with
R3025 T3770 T3768 arg1Of vector,a
R3026 T3770 T3769 arg1Of vector,retroviral
R3027 T3770 T3771 arg1Of vector,(
R3028 T3770 T3774 arg1Of vector,lacking
R3029 T3772 T3771 arg2Of pMIP,(
R3030 T3773 T3771 arg3Of ),(
R3031 T3777 T3774 arg2Of insert,lacking
R3032 T3777 T3775 arg1Of insert,a
R3033 T3777 T3776 arg1Of insert,cDNA
R3034 T3777 T3778 arg1Of insert,(
R3035 T3780 T3778 arg2Of vector,(
R3036 T3780 T3779 arg1Of vector,empty
R3037 T3781 T3778 arg3Of ),(
R347 T427 T425 arg1Of Protein,HOIL-1L
R348 T427 T426 arg1Of Protein,Interacting
R349 T427 T428 arg1Of Protein,(
R350 T427 T431 arg1Of Protein,Is
R351 T427 T432 arg1Of Protein,Essential
R352 T429 T428 arg2Of HOIP,(
R353 T430 T428 arg3Of ),(
R354 T432 T431 arg2Of Essential,Is
R355 T432 T433 arg1Of Essential,for
R356 T435 T433 arg2Of Signaling,for
R357 T435 T434 arg1Of Signaling,CD40
R358 T436 T437 arg1Of CD40,is
R359 T441 T437 arg2Of receptor,is
R360 T441 T438 arg1Of receptor,a
R361 T441 T439 arg1Of receptor,cell
R362 T441 T440 arg1Of receptor,surface
R3629 T4639 T4640 arg1Of HOIP,is
R363 T441 T442 arg1Of receptor,important
R3630 T4639 T4641 arg2Of HOIP,required
R3631 T4641 T4640 arg2Of required,is
R3632 T4641 T4642 arg1Of required,for
R3633 T4645 T4643 arg1Of upregulation,CD40-mediated
R3634 T4645 T4644 arg1Of upregulation,CD80
R3635 T4645 T4646 arg1Of upregulation,and
R3636 T4646 T4642 arg2Of and,for
R3637 T4647 T4646 arg2Of activation,and
R3638 T4647 T4648 arg1Of activation,of
R3639 T4651 T4648 arg2Of transcription,of
R364 T442 T443 arg1Of important,in
R3640 T4651 T4649 arg1Of transcription,germline
R3641 T4651 T4650 arg1Of transcription,epsilon
R3642 T4652 T4653 arg1Of Engagement,of
R3643 T4652 T4655 arg1Of Engagement,on
R3644 T4652 T4658 arg1Of Engagement,upregulates
R3645 T4654 T4653 arg2Of CD40,of
R3646 T4657 T4655 arg2Of cells,on
R3647 T4657 T4656 arg1Of cells,B
R3648 T4659 T4658 arg2Of expression,upregulates
R3649 T4659 T4660 arg1Of expression,of
R365 T445 T443 arg2Of activation,in
R3650 T4661 T4660 arg2Of CD80,of
R3651 T4661 T4662 arg1Of CD80,[
R3652 T4661 T4665 arg1Of CD80,","
R3653 T4663 T4662 arg2Of 10,[
R3654 T4664 T4662 arg3Of ],[
R3655 T4669 T4665 arg2Of protein,","
R3656 T4669 T4666 arg1Of protein,a
R3657 T4669 T4667 arg1Of protein,cell
R3658 T4669 T4668 arg1Of protein,surface
R3659 T4669 T4670 arg1Of protein,that
R366 T445 T444 arg1Of activation,the
R3660 T4669 T4671 arg1Of protein,promotes
R3661 T4672 T4673 arg1Of activation,of
R3662 T4672 T4680 arg1Of activation,and
R3663 T4675 T4673 arg2Of cells,of
R3664 T4675 T4674 arg1Of cells,T
R3665 T4675 T4676 arg1Of cells,interacting
R3666 T4676 T4677 arg1Of interacting,with
R3667 T4679 T4677 arg2Of cells,with
R3668 T4679 T4678 arg1Of cells,B
R3669 T4680 T4671 arg2Of and,promotes
R367 T445 T446 arg1Of activation,of
R3670 T4682 T4680 arg2Of APC,and
R3671 T4682 T4681 arg1Of APC,other
R3672 T4684 T4683 arg1Of begin,To
R3673 T4685 T4684 arg2Of evaluating,begin
R3674 T4687 T4685 arg2Of contributions,evaluating
R3675 T4687 T4686 arg1Of contributions,potential
R3676 T4687 T4688 arg1Of contributions,of
R3677 T4689 T4688 arg2Of HOIP,of
R3678 T4689 T4690 arg1Of HOIP,to
R3679 T4692 T4690 arg2Of signaling,to
R368 T445 T449 arg1Of activation,during
R3680 T4692 T4691 arg1Of signaling,CD40
R3681 T4694 T4684 arg1Of we,begin
R3682 T4694 T4685 arg1Of we,evaluating
R3683 T4694 T4695 arg1Of we,assayed
R3684 T4695 T4683 modOf assayed,To
R3685 T4695 T4693 arg1Of assayed,","
R3686 T4697 T4695 arg2Of upregulation,assayed
R3687 T4697 T4696 arg1Of upregulation,the
R3688 T4697 T4698 arg1Of upregulation,of
R3689 T4699 T4698 arg2Of CD80,of
R369 T448 T446 arg2Of cells,of
R3690 T4699 T4700 arg2Of CD80,induced
R3691 T4702 T4700 arg1Of engagement,induced
R3692 T4702 T4701 arg2Of engagement,by
R3693 T4702 T4703 arg1Of engagement,of
R3694 T4704 T4703 arg2Of CD40,of
R3695 T4704 T4705 arg1Of CD40,on
R3696 T4707 T4708 arg1Of A20.2J,","
R3697 T4709 T4711 arg1Of HOIP-deficient,and
R3698 T4711 T4708 arg2Of and,","
R3699 T4711 T4710 arg1Of and,","
R370 T448 T447 arg1Of cells,antigen-presenting
R3700 T4712 T4711 arg2Of HOIP-reconstituted,and
R3701 T4713 T4705 arg2Of cells,on
R3702 T4713 T4706 arg1Of cells,parental
R3703 T4713 T4707 arg1Of cells,A20.2J
R3704 T4713 T4709 arg1Of cells,HOIP-deficient
R3705 T4713 T4712 arg1Of cells,HOIP-reconstituted
R3706 T4713 T4714 arg1Of cells,(
R3707 T4715 T4714 arg2Of Fig.,(
R3708 T4715 T4716 arg1Of Fig.,2
R3709 T4717 T4714 arg3Of ),(
R371 T451 T449 arg2Of responses,during
R3710 T4719 T4718 arg2Of to,Compared
R3711 T4723 T4719 arg2Of line,to
R3712 T4723 T4720 arg1Of line,the
R3713 T4723 T4721 arg1Of line,parental
R3714 T4723 T4722 arg1Of line,cell
R3715 T4727 T4725 arg1Of upregulation,the
R3716 T4727 T4726 arg1Of upregulation,CD40-stimulated
R3717 T4727 T4728 arg1Of upregulation,of
R3718 T4727 T4730 arg1Of upregulation,was
R3719 T4727 T4732 arg2Of upregulation,reduced
R372 T451 T450 arg1Of responses,immune
R3720 T4729 T4728 arg2Of CD80,of
R3721 T4732 T4718 arg1Of reduced,Compared
R3722 T4732 T4724 arg1Of reduced,","
R3723 T4732 T4730 arg2Of reduced,was
R3724 T4732 T4731 arg1Of reduced,dramatically
R3725 T4732 T4733 arg1Of reduced,in
R3726 T4736 T4733 arg2Of cells,in
R3727 T4736 T4734 arg1Of cells,all
R3728 T4736 T4735 arg1Of cells,HOIP-deficient
R3729 T4736 T4737 arg2Of cells,tested
R373 T453 T454 arg1Of macrophages,and
R3730 T4739 T4738 arg2Of contrast,In
R3731 T4741 T4742 arg1Of upregulation,of
R3732 T4741 T4744 arg1Of upregulation,was
R3733 T4741 T4745 arg2Of upregulation,restored
R3734 T4743 T4742 arg2Of CD80,of
R3735 T4745 T4738 arg1Of restored,In
R3736 T4745 T4740 arg1Of restored,","
R3737 T4745 T4744 arg2Of restored,was
R3738 T4745 T4746 arg1Of restored,in
R3739 T4745 T4749 arg1Of restored,","
R374 T454 T452 arg2Of and,In
R3740 T4745 T4750 modOf restored,confirming
R3741 T4748 T4746 arg2Of cells,in
R3742 T4748 T4747 arg1Of cells,HOIP-reconstituted
R3743 T4753 T4752 arg1Of defect,the
R3744 T4753 T4754 arg1Of defect,in
R3745 T4753 T4757 arg1Of defect,in
R3746 T4753 T4761 arg1Of defect,was
R3747 T4753 T4762 arg1Of defect,due
R3748 T4756 T4754 arg2Of upregulation,in
R3749 T4756 T4755 arg1Of upregulation,CD80
R375 T456 T454 arg2Of cells,and
R3750 T4760 T4757 arg2Of cells,in
R3751 T4760 T4758 arg1Of cells,the
R3752 T4760 T4759 arg1Of cells,HOIP-deficient
R3753 T4761 T4750 arg2Of was,confirming
R3754 T4761 T4751 arg1Of was,that
R3755 T4762 T4761 arg2Of due,was
R3756 T4762 T4763 arg1Of due,to
R3757 T4765 T4763 arg2Of disruption,to
R3758 T4765 T4764 arg1Of disruption,the
R3759 T4765 T4766 arg1Of disruption,of
R376 T456 T455 arg1Of cells,dendritic
R3760 T4767 T4766 arg2Of Rnf31,of
R3761 T4769 T4768 arg1Of signals,CD40
R3762 T4769 T4770 arg1Of signals,in
R3763 T4769 T4773 arg1Of signals,can
R3764 T4769 T4775 arg1Of signals,contribute
R3765 T4772 T4770 arg2Of cells,in
R3766 T4772 T4771 arg1Of cells,B
R3767 T4775 T4773 arg2Of contribute,can
R3768 T4775 T4774 arg1Of contribute,also
R3769 T4775 T4776 arg1Of contribute,to
R377 T458 T459 arg1Of engagement,of
R3770 T4775 T4791 arg1Of contribute,","
R3771 T4775 T4792 arg1Of contribute,[
R3772 T4778 T4776 arg2Of activation,to
R3773 T4778 T4777 arg1Of activation,the
R3774 T4778 T4779 arg1Of activation,of
R3775 T4778 T4782 arg1Of activation,in
R3776 T4781 T4779 arg2Of recombination,of
R3777 T4781 T4780 arg1Of recombination,DNA
R3778 T4787 T4782 arg2Of locus,in
R3779 T4787 T4783 arg1Of locus,the
R378 T458 T461 arg1Of engagement,by
R3780 T4787 T4784 arg1Of locus,immunoglobulin
R3781 T4787 T4785 arg1Of locus,heavy
R3782 T4787 T4786 arg1Of locus,chain
R3783 T4787 T4788 arg1Of locus,[
R3784 T4789 T4788 arg2Of 11,[
R3785 T4790 T4788 arg3Of ],[
R3786 T4793 T4792 arg2Of 12,[
R3787 T4794 T4792 arg3Of ],[
R3788 T4796 T4795 arg1Of process,This
R3789 T4796 T4797 arg1Of process,","
R379 T458 T465 arg1Of engagement,provides
R3790 T4796 T4798 arg2Of process,known
R3791 T4796 T4809 arg1Of process,allows
R3792 T4796 T4814 arg1Of process,expressing
R3793 T4798 T4799 arg1Of known,as
R3794 T4802 T4799 arg2Of switching,as
R3795 T4802 T4800 arg1Of switching,immunoglobulin
R3796 T4802 T4801 arg1Of switching,class
R3797 T4802 T4803 arg1Of switching,(
R3798 T4806 T4803 arg2Of switching,(
R3799 T4806 T4804 arg1Of switching,or
R380 T460 T459 arg2Of CD40,of
R3800 T4806 T4805 arg1Of switching,isotype
R3801 T4807 T4803 arg3Of ),(
R3802 T4809 T4808 arg1Of allows,","
R3803 T4809 T4812 arg1Of allows,","
R3804 T4809 T4814 modOf allows,expressing
R3805 T4811 T4809 arg2Of cells,allows
R3806 T4811 T4810 arg1Of cells,B
R3807 T4814 T4813 arg1Of expressing,initially
R3808 T4814 T4817 modOf expressing,to
R3809 T4815 T4814 arg2Of IgM,expressing
R381 T464 T461 arg2Of CD154,by
R3810 T4815 T4833 arg1Of IgM,appropriate
R3811 T4818 T4816 arg1Of switch,","
R3812 T4818 T4817 arg1Of switch,to
R3813 T4818 T4819 arg1Of switch,to
R3814 T4818 T4826 arg1Of switch,depending
R3815 T4820 T4821 arg1Of IgA,","
R3816 T4821 T4824 arg1Of ",",or
R3817 T4822 T4821 arg2Of IgG,","
R3818 T4824 T4819 arg2Of or,to
R3819 T4824 T4823 arg1Of or,","
R382 T464 T462 arg1Of CD154,its
R3820 T4825 T4824 arg2Of IgE,or
R3821 T4827 T4826 arg2Of on,depending
R3822 T4829 T4827 arg2Of class,on
R3823 T4829 T4828 arg1Of class,the
R3824 T4829 T4830 arg1Of class,of
R3825 T4831 T4830 arg2Of antibody,of
R3826 T4833 T4814 arg3Of appropriate,expressing
R3827 T4833 T4832 arg1Of appropriate,most
R3828 T4833 T4834 arg1Of appropriate,for
R3829 T4838 T4834 arg2Of response,for
R383 T464 T463 arg1Of CD154,ligand
R3830 T4838 T4835 arg1Of response,a
R3831 T4838 T4836 arg1Of response,particular
R3832 T4838 T4837 arg1Of response,immune
R3833 T4839 T4840 arg1Of Switching,to
R3834 T4839 T4842 arg1Of Switching,can
R3835 T4839 T4843 arg1Of Switching,be
R3836 T4839 T4844 arg2Of Switching,induced
R3837 T4841 T4840 arg2Of IgE,to
R3838 T4844 T4842 arg2Of induced,can
R3839 T4844 T4843 arg2Of induced,be
R384 T465 T452 arg1Of provides,In
R3840 T4847 T4844 arg1Of combination,induced
R3841 T4847 T4845 arg2Of combination,by
R3842 T4847 T4846 arg1Of combination,the
R3843 T4847 T4848 arg1Of combination,of
R3844 T4849 T4850 arg1Of IL-4,and
R3845 T4851 T4850 arg2Of CD40,and
R3846 T4852 T4848 arg2Of signals,of
R3847 T4852 T4849 arg1Of signals,IL-4
R3848 T4852 T4851 arg1Of signals,CD40
R3849 T4855 T4853 arg1Of rearrangement,The
R385 T465 T457 arg1Of provides,","
R3850 T4855 T4854 arg1Of rearrangement,gene
R3851 T4855 T4856 arg1Of rearrangement,necessary
R3852 T4855 T4860 arg1Of rearrangement,is
R3853 T4855 T4861 arg2Of rearrangement,preceded
R3854 T4856 T4857 arg1Of necessary,for
R3855 T4859 T4857 arg2Of production,for
R3856 T4859 T4858 arg1Of production,IgE
R3857 T4861 T4860 arg2Of preceded,is
R3858 T4864 T4861 arg1Of production,preceded
R3859 T4864 T4862 arg2Of production,by
R386 T465 T475 arg1Of provides,","
R3860 T4864 T4863 arg1Of production,the
R3861 T4864 T4865 arg1Of production,of
R3862 T4868 T4865 arg2Of transcripts,of
R3863 T4868 T4866 arg1Of transcripts,non-coding
R3864 T4868 T4867 arg1Of transcripts,RNA
R3865 T4868 T4869 arg1Of transcripts,from
R3866 T4871 T4869 arg2Of sequences,from
R3867 T4871 T4870 arg1Of sequences,germline
R3868 T4871 T4872 arg1Of sequences,in
R3869 T4877 T4872 arg2Of locus,in
R387 T465 T476 arg1Of provides,respectively
R3870 T4877 T4873 arg1Of locus,the
R3871 T4877 T4874 arg1Of locus,IgE
R3872 T4877 T4875 arg1Of locus,heavy
R3873 T4877 T4876 arg1Of locus,chain
R3874 T4877 T4878 arg1Of locus,[
R3875 T4879 T4878 arg2Of 13,[
R3876 T4880 T4878 arg3Of ],[
R3877 T4882 T4881 arg1Of determine,To
R3878 T4884 T4885 arg1Of HOIP,is
R3879 T4884 T4886 arg2Of HOIP,required
R388 T466 T465 arg2Of signals,provides
R3880 T4886 T4882 arg2Of required,determine
R3881 T4886 T4883 arg1Of required,whether
R3882 T4886 T4885 arg2Of required,is
R3883 T4886 T4887 arg1Of required,in
R3884 T4889 T4887 arg2Of initiation,in
R3885 T4889 T4888 arg1Of initiation,the
R3886 T4889 T4890 arg1Of initiation,of
R3887 T4894 T4890 arg2Of process,of
R3888 T4894 T4891 arg1Of process,the
R3889 T4894 T4892 arg1Of process,class
R389 T466 T467 arg1Of signals,critical
R3890 T4894 T4893 arg1Of process,switching
R3891 T4900 T4896 arg1Of lines,the
R3892 T4900 T4897 arg1Of lines,relevant
R3893 T4900 T4898 arg1Of lines,B
R3894 T4900 T4899 arg1Of lines,cell
R3895 T4900 T4901 arg1Of lines,were
R3896 T4900 T4902 arg2Of lines,stimulated
R3897 T4900 T4911 arg2Of lines,tested
R3898 T4902 T4903 arg1Of stimulated,through
R3899 T4902 T4905 arg1Of stimulated,in
R390 T467 T468 arg1Of critical,for
R3900 T4902 T4910 arg1Of stimulated,and
R3901 T4904 T4903 arg2Of CD40,through
R3902 T4907 T4905 arg2Of presence,in
R3903 T4907 T4906 arg1Of presence,the
R3904 T4907 T4908 arg1Of presence,of
R3905 T4909 T4908 arg2Of IL-4,of
R3906 T4910 T4881 modOf and,To
R3907 T4910 T4895 arg1Of and,","
R3908 T4910 T4901 arg2Of and,were
R3909 T4911 T4910 arg2Of tested,and
R391 T469 T470 arg1Of anti-microbial,and
R3910 T4911 T4912 arg1Of tested,for
R3911 T4913 T4912 arg2Of production,for
R3912 T4913 T4914 arg1Of production,of
R3913 T4916 T4915 arg1Of epsilon,germline
R3914 T4916 T4917 arg1Of epsilon,(
R3915 T4918 T4917 arg2Of GLε,(
R3916 T4919 T4917 arg3Of ),(
R3917 T4920 T4914 arg2Of transcripts,of
R3918 T4920 T4916 arg1Of transcripts,epsilon
R3919 T4920 T4921 arg1Of transcripts,(
R392 T472 T470 arg2Of cell-mediated,and
R3920 T4922 T4921 arg2Of Fig.,(
R3921 T4922 T4923 arg1Of Fig.,3
R3922 T4924 T4921 arg3Of ),(
R3923 T4927 T4925 arg1Of stimulation,Anti-CD40
R3924 T4927 T4926 arg1Of stimulation,antibody
R3925 T4927 T4928 arg1Of stimulation,alone
R3926 T4927 T4930 arg1Of stimulation,together
R3927 T4927 T4933 arg1Of stimulation,induced
R3928 T4928 T4929 arg1Of alone,or
R3929 T4929 T4931 arg1Of or,with
R393 T472 T471 arg1Of cell-mediated,T
R3930 T4930 T4929 arg2Of together,or
R3931 T4932 T4931 arg2Of IL-4,with
R3932 T4935 T4933 arg2Of transcription,induced
R3933 T4935 T4934 arg1Of transcription,GLε
R3934 T4935 T4936 arg1Of transcription,in
R3935 T4939 T4936 arg2Of cells,in
R3936 T4939 T4937 arg1Of cells,parental
R3937 T4939 T4938 arg1Of cells,A20.2J
R3938 T4940 T4933 arg3Of as,induced
R3939 T4941 T4940 arg2Of expected,as
R394 T474 T468 arg2Of responses,for
R3940 T4943 T4942 arg2Of contrast,In
R3941 T4947 T4945 arg1Of levels,only
R3942 T4947 T4946 arg1Of levels,low
R3943 T4947 T4948 arg1Of levels,of
R3944 T4947 T4951 arg1Of levels,were
R3945 T4947 T4952 arg2Of levels,detected
R3946 T4950 T4948 arg2Of expression,of
R3947 T4950 T4949 arg1Of expression,GLε
R3948 T4952 T4942 arg1Of detected,In
R3949 T4952 T4944 arg1Of detected,","
R395 T474 T469 arg1Of responses,anti-microbial
R3950 T4952 T4951 arg2Of detected,were
R3951 T4952 T4953 arg1Of detected,in
R3952 T4955 T4953 arg2Of cells,in
R3953 T4955 T4954 arg1Of cells,HOIP-deficient
R3954 T4955 T4956 arg2Of cells,stimulated
R3955 T4956 T4957 arg1Of stimulated,with
R3956 T4960 T4959 arg1Of antibody,anti-CD40
R3957 T4960 T4961 arg1Of antibody,alone
R3958 T4960 T4962 arg1Of antibody,or
R3959 T4962 T4957 arg2Of or,with
R396 T474 T472 arg1Of responses,cell-mediated
R3960 T4962 T4958 arg1Of or,either
R3961 T4963 T4964 arg1Of anti-CD40,and
R3962 T4964 T4962 arg2Of and,or
R3963 T4965 T4964 arg2Of IL-4,and
R3964 T4967 T4966 arg1Of levels,Normal
R3965 T4967 T4968 arg1Of levels,of
R3966 T4967 T4971 arg1Of levels,were
R3967 T4967 T4972 arg2Of levels,induced
R3968 T4970 T4968 arg2Of expression,of
R3969 T4970 T4969 arg1Of expression,GLε
R397 T474 T473 arg1Of responses,immune
R3970 T4972 T4971 arg2Of induced,were
R3971 T4972 T4973 arg1Of induced,in
R3972 T4975 T4973 arg2Of cells,in
R3973 T4975 T4974 arg1Of cells,HOIP-reconstituted
R3974 T4975 T4976 arg2Of cells,stimulated
R3975 T4976 T4977 arg1Of stimulated,with
R3976 T4979 T4977 arg2Of antibody,with
R3977 T4979 T4978 arg1Of antibody,anti-CD40
R3978 T4979 T4980 arg1Of antibody,alone
R3979 T4979 T4982 arg1Of antibody,together
R398 T479 T477 arg2Of cells,In
R3980 T4980 T4981 arg1Of alone,or
R3981 T4981 T4983 arg1Of or,with
R3982 T4982 T4981 arg2Of together,or
R3983 T4984 T4983 arg2Of IL-4,with
R3984 T4988 T4987 arg1Of data,these
R3985 T4988 T4989 arg1Of data,demonstrate
R3986 T4989 T4985 arg1Of demonstrate,Collectively
R3987 T4989 T4986 arg1Of demonstrate,","
R3988 T4991 T4992 arg1Of HOIP,has
R3989 T4992 T4989 arg2Of has,demonstrate
R399 T479 T478 arg1Of cells,B
R3990 T4992 T4990 arg1Of has,that
R3991 T4992 T4996 arg1Of has,in
R3992 T4995 T4992 arg2Of role,has
R3993 T4995 T4993 arg1Of role,a
R3994 T4995 T4994 arg1Of role,critical
R3995 T4998 T4996 arg2Of responses,in
R3996 T4998 T4997 arg1Of responses,cellular
R3997 T4998 T4999 arg1Of responses,to
R3998 T5001 T4999 arg2Of signaling,to
R3999 T5001 T5000 arg1Of signaling,CD40
R400 T482 T481 arg1Of signaling,CD40
R401 T482 T483 arg1Of signaling,has
R402 T483 T477 arg1Of has,In
R403 T483 T480 arg1Of has,","
R404 T486 T483 arg2Of role,has
R405 T486 T484 arg1Of role,a
R406 T486 T485 arg1Of role,major
R407 T486 T487 arg1Of role,in
R408 T488 T487 arg2Of regulating,in
R409 T490 T489 arg1Of proliferation,cell
R410 T490 T491 arg1Of proliferation,","
R411 T491 T495 arg1Of ",",and
R412 T493 T491 arg2Of production,","
R413 T493 T492 arg1Of production,antibody
R414 T495 T488 arg2Of and,regulating
R415 T495 T494 arg1Of and,","
R416 T499 T495 arg2Of development,and
R417 T499 T496 arg1Of development,memory
R418 T499 T497 arg1Of development,B
R419 T499 T498 arg1Of development,cell
R420 T501 T500 arg1Of engagement,CD40
R421 T501 T502 arg1Of engagement,results
R422 T502 T503 arg1Of results,in
R423 T505 T503 arg2Of formation,in
R424 T505 T504 arg1Of formation,the
R425 T505 T506 arg1Of formation,of
R426 T505 T510 arg1Of formation,that
R427 T505 T511 arg1Of formation,mediates
R428 T509 T506 arg2Of complex,of
R429 T509 T507 arg1Of complex,a
R430 T509 T508 arg1Of complex,receptor-associated
R431 T512 T511 arg2Of activation,mediates
R432 T512 T513 arg1Of activation,of
R433 T514 T515 arg1Of NF-κB,","
R434 T515 T520 arg1Of ",",and
R435 T518 T515 arg2Of kinases,","
R436 T518 T516 arg1Of kinases,stress-activated
R437 T518 T517 arg1Of kinases,protein
R438 T520 T513 arg2Of and,of
R439 T520 T519 arg1Of and,","
R440 T523 T520 arg2Of molecules,and
R441 T523 T521 arg1Of molecules,other
R442 T523 T522 arg1Of molecules,signaling
R443 T527 T526 arg1Of mechanisms,the
R444 T527 T528 arg1Of mechanisms,that
R445 T527 T529 arg1Of mechanisms,link
R446 T527 T535 arg1Of mechanisms,have
R447 T527 T536 arg1Of mechanisms,been
R448 T527 T539 arg2Of mechanisms,characterized
R4483 T5789 T5790 arg1Of HOIP,mediates
R4484 T5792 T5791 arg1Of NF-κB,CD40-stimulated
R4485 T5792 T5793 arg1Of NF-κB,and
R4486 T5793 T5790 arg2Of and,mediates
R4487 T5795 T5793 arg2Of activation,and
R4488 T5795 T5794 arg1Of activation,JNK
R4489 T5797 T5796 arg1Of results,The
R449 T529 T531 arg1Of link,to
R4490 T5797 T5798 arg2Of results,described
R4491 T5797 T5800 arg1Of results,show
R4492 T5798 T5799 arg1Of described,above
R4493 T5802 T5803 arg1Of HOIP,plays
R4494 T5803 T5800 arg2Of plays,show
R4495 T5803 T5801 arg1Of plays,that
R4496 T5803 T5807 arg1Of plays,in
R4497 T5806 T5803 arg2Of role,plays
R4498 T5806 T5804 arg1Of role,an
R4499 T5806 T5805 arg1Of role,important
R450 T530 T529 arg2Of CD40,link
R4500 T5810 T5807 arg2Of functions,in
R4501 T5810 T5808 arg1Of functions,CD40-mediated
R4502 T5810 T5809 arg1Of functions,effector
R4503 T5810 T5811 arg1Of functions,of
R4504 T5813 T5811 arg2Of cells,of
R4505 T5813 T5812 arg1Of cells,B
R4506 T5817 T5818 arg1Of HOIP,is
R4507 T5818 T5814 arg1Of is,It
R4508 T5818 T5815 arg1Of is,follows
R4509 T5818 T5816 arg1Of is,that
R451 T534 T531 arg2Of events,to
R4510 T5818 T5819 arg1Of is,likely
R4511 T5822 T5818 arg2Of mediator,is
R4512 T5822 T5820 arg1Of mediator,a
R4513 T5822 T5821 arg1Of mediator,key
R4514 T5822 T5823 arg1Of mediator,of
R4515 T5825 T5823 arg2Of signaling,of
R4516 T5825 T5824 arg1Of signaling,CD40
R4517 T5827 T5826 arg1Of test,To
R4518 T5829 T5827 arg2Of hypothesis,test
R4519 T5829 T5828 arg1Of hypothesis,this
R452 T534 T532 arg1Of events,these
R4520 T5831 T5827 arg1Of we,test
R4521 T5831 T5832 arg1Of we,stimulated
R4522 T5832 T5826 modOf stimulated,To
R4523 T5832 T5830 arg1Of stimulated,","
R4524 T5833 T5834 arg1Of A20.2J,and
R4525 T5834 T5832 arg2Of and,stimulated
R4526 T5834 T5837 arg1Of and,with
R4527 T5836 T5834 arg2Of cells,and
R4528 T5836 T5835 arg1Of cells,HOIP-deficient
R4529 T5838 T5839 arg1Of CD154,(
R453 T534 T533 arg1Of events,signaling
R4530 T5838 T5843 arg2Of CD154,expressed
R4531 T5838 T5855 arg1Of CD154,and
R4532 T5841 T5839 arg2Of ligand,(
R4533 T5841 T5840 arg1Of ligand,CD40
R4534 T5842 T5839 arg3Of ),(
R4535 T5843 T5851 arg1Of expressed,","
R4536 T5843 T5852 arg1Of expressed,[
R4537 T5847 T5843 arg1Of cells,expressed
R4538 T5847 T5844 arg2Of cells,by
R4539 T5847 T5845 arg1Of cells,HI5
R454 T538 T537 arg1Of partially,only
R4540 T5847 T5846 arg1Of cells,insect
R4541 T5847 T5848 arg1Of cells,[
R4542 T5849 T5848 arg2Of 7,[
R4543 T5850 T5848 arg3Of ],[
R4544 T5853 T5852 arg2Of 8,[
R4545 T5854 T5852 arg3Of ],[
R4546 T5855 T5837 arg2Of and,with
R4547 T5857 T5855 arg2Of activation,and
R4548 T5857 T5856 arg2Of activation,measured
R4549 T5857 T5858 arg1Of activation,of
R455 T539 T524 arg1Of characterized,However
R4550 T5860 T5861 arg1Of NF-κB,and
R4551 T5862 T5861 arg2Of JNK,and
R4552 T5863 T5858 arg2Of pathways,of
R4553 T5863 T5859 arg1Of pathways,the
R4554 T5863 T5860 arg1Of pathways,NF-κB
R4555 T5863 T5862 arg1Of pathways,JNK
R4556 T5863 T5864 arg1Of pathways,","
R4557 T5865 T5864 arg2Of two,","
R4558 T5865 T5866 arg1Of two,of
R4559 T5870 T5866 arg2Of regulators,of
R456 T539 T525 arg1Of characterized,","
R4560 T5870 T5867 arg1Of regulators,the
R4561 T5870 T5868 arg1Of regulators,major
R4562 T5870 T5869 arg1Of regulators,transcriptional
R4563 T5870 T5871 arg2Of regulators,activated
R4564 T5871 T5874 arg1Of activated,[
R4565 T5873 T5871 arg1Of CD40,activated
R4566 T5873 T5872 arg2Of CD40,by
R4567 T5875 T5874 arg2Of 4,[
R4568 T5876 T5874 arg3Of ],[
R4569 T5878 T5877 arg1Of CD154,Cell-associated
R457 T539 T535 arg2Of characterized,have
R4570 T5878 T5879 arg1Of CD154,was
R4571 T5878 T5880 arg2Of CD154,used
R4572 T5880 T5879 arg2Of used,was
R4573 T5880 T5881 arg1Of used,as
R4574 T5883 T5881 arg2Of stimulus,as
R4575 T5883 T5882 arg1Of stimulus,the
R4576 T5883 T5884 arg1Of stimulus,in
R4577 T5886 T5884 arg2Of experiments,in
R4578 T5886 T5885 arg1Of experiments,these
R4579 T5886 T5887 arg1Of experiments,as
R458 T539 T536 arg2Of characterized,been
R4580 T5888 T5890 arg1Of it,provides
R4581 T5890 T5887 arg2Of provides,as
R4582 T5890 T5889 arg1Of provides,typically
R4583 T5892 T5891 arg1Of robust,more
R4584 T5892 T5894 arg1Of robust,and
R4585 T5894 T5893 arg1Of and,","
R4586 T5896 T5894 arg2Of more,and
R4587 T5896 T5895 arg1Of more,therefore
R4588 T5898 T5897 arg1Of detected,readily
R4589 T5901 T5890 arg2Of signals,provides
R459 T539 T538 arg1Of characterized,partially
R4590 T5901 T5892 arg1Of signals,robust
R4591 T5901 T5896 arg1Of signals,more
R4592 T5901 T5898 arg2Of signals,detected
R4593 T5901 T5899 arg1Of signals,","
R4594 T5901 T5900 arg1Of signals,activation
R4595 T5901 T5902 arg1Of signals,than
R4596 T5903 T5902 arg2Of does,than
R4597 T5905 T5903 arg1Of antibody,does
R4598 T5905 T5904 arg1Of antibody,anti-CD40
R4599 T5906 T5907 arg1Of Activation,of
R460 T541 T540 arg2Of components,Known
R4600 T5906 T5912 arg1Of Activation,is
R4601 T5906 T5913 arg2Of Activation,initiated
R4602 T5911 T5907 arg2Of pathway,of
R4603 T5911 T5908 arg1Of pathway,the
R4604 T5911 T5909 arg1Of pathway,canonical
R4605 T5911 T5910 arg1Of pathway,NF-κB
R4606 T5913 T5912 arg2Of initiated,is
R4607 T5913 T5914 arg1Of initiated,with
R4608 T5916 T5914 arg2Of phosphorylation,with
R4609 T5916 T5915 arg1Of phosphorylation,the
R461 T541 T542 arg1Of components,of
R4610 T5916 T5917 arg1Of phosphorylation,of
R4611 T5916 T5920 arg1Of phosphorylation,by
R4612 T5919 T5917 arg2Of proteins,of
R4613 T5919 T5918 arg1Of proteins,IκB
R4614 T5924 T5920 arg2Of complex,by
R4615 T5924 T5921 arg1Of complex,the
R4616 T5924 T5922 arg1Of complex,IκB
R4617 T5924 T5923 arg1Of complex,kinase
R4618 T5924 T5925 arg1Of complex,(
R4619 T5926 T5925 arg2Of IKK,(
R462 T541 T547 arg1Of components,include
R4620 T5927 T5925 arg3Of ),(
R4621 T5930 T5928 arg2Of cells,In
R4622 T5930 T5929 arg1Of cells,resting
R4623 T5933 T5932 arg1Of proteins,IκB
R4624 T5933 T5934 arg1Of proteins,are
R4625 T5933 T5935 arg1Of proteins,responsible
R4626 T5934 T5928 arg1Of are,In
R4627 T5934 T5931 arg1Of are,","
R4628 T5935 T5934 arg2Of responsible,are
R4629 T5935 T5936 arg1Of responsible,for
R463 T546 T542 arg2Of complex,of
R4630 T5937 T5936 arg2Of sequestering,for
R4631 T5937 T5940 arg1Of sequestering,in
R4632 T5939 T5937 arg2Of subunits,sequestering
R4633 T5939 T5938 arg1Of subunits,NF-κB
R4634 T5942 T5940 arg2Of cytoplasm,in
R4635 T5942 T5941 arg1Of cytoplasm,the
R4636 T5943 T5944 arg1Of Phosphorylation,by
R4637 T5943 T5948 arg1Of Phosphorylation,targets
R4638 T5947 T5944 arg2Of complex,by
R4639 T5947 T5945 arg1Of complex,the
R464 T546 T543 arg1Of complex,the
R4640 T5947 T5946 arg1Of complex,IKK
R4641 T5948 T5955 arg1Of targets,","
R4642 T5948 T5956 modOf targets,allowing
R4643 T5950 T5948 arg2Of proteins,targets
R4644 T5950 T5949 arg1Of proteins,IκB
R4645 T5950 T5951 arg1Of proteins,for
R4646 T5952 T5953 arg1Of ubiquitination,and
R4647 T5953 T5951 arg2Of and,for
R4648 T5954 T5953 arg2Of degradation,and
R4649 T5957 T5956 arg2Of NF-κB,allowing
R465 T546 T544 arg1Of complex,CD40
R4650 T5957 T5959 arg1Of NF-κB,enter
R4651 T5957 T5963 arg1Of NF-κB,activate
R4652 T5959 T5962 arg1Of enter,and
R4653 T5961 T5959 arg2Of nucleus,enter
R4654 T5961 T5960 arg1Of nucleus,the
R4655 T5962 T5956 arg3Of and,allowing
R4656 T5962 T5958 arg1Of and,to
R4657 T5963 T5962 arg2Of activate,and
R4658 T5965 T5963 arg2Of expression,activate
R4659 T5965 T5964 arg1Of expression,gene
R466 T546 T545 arg1Of complex,signaling
R4660 T5967 T5966 arg1Of phosphorylation,CD40-mediated
R4661 T5967 T5968 arg1Of phosphorylation,and
R4662 T5968 T5972 arg1Of and,in
R4663 T5968 T5975 arg1Of and,was
R4664 T5968 T5977 arg1Of and,impaired
R4665 T5969 T5968 arg2Of degradation,and
R4666 T5969 T5970 arg1Of degradation,of
R4667 T5971 T5970 arg2Of IκBα,of
R4668 T5974 T5972 arg2Of cells,in
R4669 T5974 T5973 arg1Of cells,HOIP-deficient
R467 T548 T547 arg2Of members,include
R4670 T5977 T5975 arg2Of impaired,was
R4671 T5977 T5976 arg1Of impaired,dramatically
R4672 T5977 T5979 arg1Of impaired,to
R4673 T5979 T5978 arg1Of to,relative
R4674 T5980 T5979 arg2Of that,to
R4675 T5980 T5981 arg2Of that,observed
R4676 T5981 T5982 arg1Of observed,in
R4677 T5985 T5982 arg2Of cells,in
R4678 T5985 T5983 arg1Of cells,parental
R4679 T5985 T5984 arg1Of cells,A20.2J
R468 T548 T549 arg1Of members,of
R4680 T5985 T5986 arg1Of cells,(
R4681 T5987 T5986 arg2Of Fig.,(
R4682 T5987 T5988 arg1Of Fig.,4
R4683 T5989 T5986 arg3Of ),(
R4684 T5990 T5992 arg1Of We,assayed
R4685 T5992 T5991 arg1Of assayed,also
R4686 T5992 T6000 arg1Of assayed,in
R4687 T5993 T5992 arg2Of activation,assayed
R4688 T5993 T5994 arg1Of activation,of
R4689 T5999 T5994 arg2Of JNK,of
R469 T553 T551 arg1Of factor,TNF
R4690 T5999 T5995 arg1Of JNK,the
R4691 T5999 T5996 arg1Of JNK,stress-activated
R4692 T5999 T5997 arg1Of JNK,protein
R4693 T5999 T5998 arg1Of JNK,kinase
R4694 T6001 T6000 arg2Of response,in
R4695 T6001 T6002 arg1Of response,to
R4696 T6004 T6002 arg2Of engagement,to
R4697 T6004 T6003 arg1Of engagement,CD40
R4698 T6004 T6005 arg1Of engagement,(
R4699 T6006 T6005 arg2Of Fig.,(
R470 T553 T552 arg1Of factor,receptor-associated
R4700 T6006 T6007 arg1Of Fig.,4
R4701 T6008 T6005 arg3Of ),(
R4702 T6011 T6009 arg1Of activation,CD40-mediated
R4703 T6011 T6010 arg1Of activation,JNK
R4704 T6011 T6012 arg1Of activation,in
R4705 T6011 T6015 arg1Of activation,was
R4706 T6011 T6016 arg2Of activation,impaired
R4707 T6014 T6012 arg2Of cells,in
R4708 T6014 T6013 arg1Of cells,HOIP-deficient
R4709 T6016 T6015 arg2Of impaired,was
R471 T553 T554 arg1Of factor,(
R4710 T6016 T6017 arg1Of impaired,as
R4711 T6018 T6017 arg2Of measured,as
R4712 T6020 T6018 arg1Of phosphorylation,measured
R4713 T6020 T6019 arg2Of phosphorylation,by
R4714 T6020 T6021 arg1Of phosphorylation,of
R4715 T6022 T6023 arg1Of Thr183,and
R4716 T6023 T6021 arg2Of and,of
R4717 T6023 T6025 arg1Of and,in
R4718 T6024 T6023 arg2Of Tyr185,and
R4719 T6026 T6025 arg2Of JNK,in
R472 T555 T554 arg2Of TRAF,(
R4720 T6028 T6027 arg1Of activation,CD40-induced
R4721 T6028 T6029 arg1Of activation,of
R4722 T6028 T6033 arg1Of activation,in
R4723 T6028 T6036 arg1Of activation,was
R4724 T6028 T6037 arg1Of activation,normal
R4725 T6030 T6031 arg1Of NF-κB,and
R4726 T6031 T6029 arg2Of and,of
R4727 T6032 T6031 arg2Of JNK,and
R4728 T6035 T6033 arg2Of cells,in
R4729 T6035 T6034 arg1Of cells,HOIP-reconstituted
R473 T556 T554 arg3Of ),(
R4730 T6036 T6038 arg1Of was,","
R4731 T6036 T6039 modOf was,demonstrating
R4732 T6037 T6036 arg2Of normal,was
R4733 T6042 T6041 arg1Of defects,the
R4734 T6042 T6043 arg2Of defects,observed
R4735 T6042 T6047 arg1Of defects,were
R4736 T6042 T6048 arg1Of defects,due
R4737 T6043 T6044 arg1Of observed,in
R4738 T6046 T6044 arg2Of cells,in
R4739 T6046 T6045 arg1Of cells,gene-deficient
R474 T557 T549 arg2Of family,of
R4740 T6047 T6039 arg2Of were,demonstrating
R4741 T6047 T6040 arg1Of were,that
R4742 T6048 T6047 arg2Of due,were
R4743 T6048 T6049 arg1Of due,to
R4744 T6051 T6049 arg2Of absence,to
R4745 T6051 T6050 arg1Of absence,the
R4746 T6051 T6052 arg1Of absence,of
R4747 T6054 T6052 arg2Of expression,of
R4748 T6054 T6053 arg1Of expression,HOIP
R475 T557 T550 arg1Of family,the
R476 T557 T553 arg1Of family,factor
R477 T557 T558 arg1Of family,of
R478 T559 T558 arg2Of proteins,of
R479 T560 T562 arg1Of We,showed
R480 T562 T561 arg1Of showed,previously
R481 T568 T564 arg1Of TRAF2,the
R482 T568 T565 arg1Of TRAF2,TRAF
R483 T568 T566 arg1Of TRAF2,family
R484 T568 T567 arg1Of TRAF2,member
R485 T568 T569 arg1Of TRAF2,mediates
R486 T569 T562 arg2Of mediates,showed
R487 T569 T563 arg1Of mediates,that
R488 T569 T583 arg1Of mediates,","
R489 T570 T569 arg2Of recruitment,mediates
R490 T570 T571 arg1Of recruitment,of
R491 T570 T577 arg1Of recruitment,to
R492 T573 T571 arg2Of protein,of
R493 T573 T572 arg1Of protein,HOIL-1L-interacting
R494 T573 T574 arg1Of protein,(
R495 T575 T574 arg2Of HOIP,(
R496 T576 T574 arg3Of ),(
R497 T580 T577 arg2Of domain,to
R498 T580 T578 arg1Of domain,the
R499 T580 T579 arg1Of domain,cytoplasmic
R500 T580 T581 arg1Of domain,of
R501 T582 T581 arg2Of CD40,of
R502 T584 T569 arg3Of suggesting,mediates
R503 T586 T587 arg1Of HOIP,has
R504 T587 T584 arg2Of has,suggesting
R505 T587 T585 arg1Of has,that
R506 T587 T590 arg1Of has,in
R507 T589 T587 arg2Of role,has
R508 T589 T588 arg1Of role,a
R509 T594 T590 arg2Of pathway,in
R510 T594 T591 arg1Of pathway,the
R511 T594 T592 arg1Of pathway,CD40
R512 T594 T593 arg1Of pathway,signaling
R513 T596 T595 arg1Of determine,To
R514 T598 T596 arg2Of role,determine
R515 T598 T597 arg1Of role,the
R516 T598 T599 arg1Of role,of
R517 T598 T601 arg1Of role,in
R518 T600 T599 arg2Of HOIP,of
R519 T603 T601 arg2Of signaling,in
R520 T603 T602 arg1Of signaling,CD40
R521 T605 T606 arg1Of we,used
R522 T606 T595 modOf used,To
R523 T606 T604 arg1Of used,","
R524 T606 T611 modOf used,to
R525 T609 T606 arg2Of gene,used
R526 T609 T607 arg1Of gene,somatic
R527 T609 T608 arg1Of gene,cell
R528 T610 T606 arg3Of targeting,used
R529 T612 T611 arg1Of generate,to
R530 T616 T612 arg2Of lines,generate
R531 T616 T613 arg1Of lines,mouse
R532 T616 T614 arg1Of lines,B
R533 T616 T615 arg1Of lines,cell
R534 T616 T617 arg1Of lines,deficient
R535 T617 T618 arg1Of deficient,in
R536 T619 T618 arg2Of HOIP,in
R537 T620 T621 arg1Of We,found
R538 T625 T623 arg1Of upregulation,the
R539 T625 T624 arg1Of upregulation,CD40-induced
R540 T625 T626 arg1Of upregulation,of
R541 T625 T628 arg1Of upregulation,and
R542 T627 T626 arg2Of CD80,of
R543 T628 T635 arg1Of and,were
R544 T628 T636 arg1Of and,defective
R545 T629 T628 arg2Of activation,and
R546 T629 T630 arg1Of activation,of
R547 T634 T630 arg2Of transcription,of
R548 T634 T631 arg1Of transcription,germline
R549 T634 T632 arg1Of transcription,immunoglobulin
R550 T634 T633 arg1Of transcription,epsilon
R551 T635 T621 arg2Of were,found
R552 T635 T622 arg1Of were,that
R553 T635 T637 arg1Of were,in
R554 T636 T635 arg2Of defective,were
R555 T639 T637 arg2Of cells,in
R556 T639 T638 arg1Of cells,HOIP-deficient
R557 T640 T642 arg1Of We,found
R558 T642 T641 arg1Of found,also
R559 T646 T644 arg1Of activation,the
R560 T646 T645 arg1Of activation,CD40-mediated
R561 T646 T647 arg1Of activation,of
R562 T646 T652 arg1Of activation,was
R563 T646 T653 arg1Of activation,impaired
R564 T648 T649 arg1Of NF-κB,and
R565 T650 T649 arg2Of c-Jun,and
R566 T651 T647 arg2Of kinase,of
R567 T651 T648 arg1Of kinase,NF-κB
R568 T651 T650 arg1Of kinase,c-Jun
R569 T652 T642 arg2Of was,found
R570 T652 T643 arg1Of was,that
R571 T653 T652 arg2Of impaired,was
R572 T654 T655 arg1Of Recruitment,of
R573 T654 T659 arg1Of Recruitment,to
R574 T654 T664 arg1Of Recruitment,was
R575 T654 T665 arg1Of Recruitment,undetectable
R576 T658 T655 arg2Of proteins,of
R577 T658 T656 arg1Of proteins,IκB
R578 T658 T657 arg1Of proteins,kinase
R579 T663 T659 arg2Of complex,to
R580 T663 T660 arg1Of complex,the
R581 T663 T661 arg1Of complex,CD40
R582 T663 T662 arg1Of complex,signaling
R583 T664 T666 arg1Of was,in
R5833 T7475 T7476 arg1Of HOIP,is
R5834 T7475 T7477 arg1Of HOIP,necessary
R5835 T7477 T7476 arg2Of necessary,is
R5836 T7477 T7478 arg1Of necessary,for
R5837 T7479 T7478 arg2Of association,for
R5838 T7479 T7480 arg1Of association,of
R5839 T7479 T7484 arg1Of association,with
R584 T664 T669 arg1Of was,","
R5840 T7483 T7480 arg2Of complex,of
R5841 T7483 T7481 arg1Of complex,the
R5842 T7483 T7482 arg1Of complex,"IκB kinase (IKK) complex with CD40 was undetectable in HOIP-deficient cells. Together, our results indicate that HOIP plays a critical role in the activation of signaling pathways that regulate cellular responses to CD40 engagement. Results Generation of HOIP-deficient B cells via targeted disruption of Rnf31, the gene encoding HOIP In our previous study, we demonstrated that HOIP is recruited to the CD40 signaling complex in two mouse B cell lines [6]. Similar results were obtained with mouse splenocytes, indicating that the interaction of CD40 with HOIP is not limited to transformed B cell lines (unpublished observations). We also found that the recruitment of HOIP to CD40 was TRAF2-dependent and that overexpression of a truncated HOIP mutant partially inhibited CD40-mediated NF-κB activation. These results support the hypothesis that HOIP plays a role in CD40 signal transduction. To further define the role and evaluate the importance of HOIP in CD40 signaling, we used somatic cell gene targeting to disrupt the gene encoding HOIP in the mouse B cell line A20.2J. This cell line has been particularly useful in the characterization of CD40 signaling mechanisms due to the relative ease with which its genome can be modified by homologous recombination [7], [8], [9]. We used a targeting vector capable of undergoing homologous recombination with Rnf31 (the gene encoding HOIP) to disrupt the coding sequence of the gene in exon 5 (Fig. 1A). Following introduction of the vector, the neomycin-resistant clones that arose were screened by PCR amplification of genomic DNA to identify cells containing a disrupted Rnf31 allele. To remove the selectable marker gene cassette from the disrupted Rnf31 allele, recombinant cell lines were transiently transfected with a plasmid that encodes Cre recombinase. This step allowed us to perform a second round of targeting and drug selection, generating cells in which both copies of Rnf31 were disrupted. Two independent clonal cell lines were chosen for further analysis. HOIP protein expression was undetectable in both cell lines, as determined by Western blot analysis of cell lysates (Fig. 1B), demonstrating that the targeting process was successful. In the text that follows, the two gene-targeted cell lines are referred to as HOIP-deficient cells. 10.1371/journal.pone.0023061.g001 Figure 1 HOIP gene targeting. (A) Regions of Rnf31 gene sequence (bottom) used in the HOIP gene targeting vector (top) are shown. Arrows (F and R) indicate approximate positions of sequences homologous to oligonucleotides used for PCR-mediated detection of homologous recombination. Homologous recombination of the vector with Rnf31 resulted in a small chromosomal deletion and the in-frame insertion of neomycin phosphotransferase (NeoR) into the amino-terminal HOIP coding sequence. A diphtheria toxin (DT) cassette in the vector facilitates the negative selection of cells in which random chromosomal insertion of the vector takes place. LoxP sequences allow the Cre-mediated deletion of the NeoR coding sequence. The SV40pA sequence helps to ensure disruption of gene expression after deletion of the NeoR sequence. (B) Anti-HOIP and anti-FLAG Western blots of cell lysates from A20.2J cells and HOIP-deficient (HOIP-/-) clones. A partial decrease in HOIP protein expression is evident in cells following disruption of one copy of Rnf31 (HOIP−/+). HOIP expression in clones reconstituted with an empty retroviral vector (pMIP) or a retroviral vector encoding FLAG-tagged HOIP is also shown. Approximate molecular weight of HOIP is 120 kD. To enable us to confirm that any signaling or functional defects observed in HOIP-deficient cells were due to disruption of the Rnf31 gene, both HOIP-deficient cell lines were transduced with a retrovirus encoding FLAG-tagged wild-type HOIP, thus restoring HOIP protein expression (Fig. 1B). These cells are referred to as HOIP-reconstituted cells. In the experiments that follow, responses by these cells were compared to those of A20.2J cells and HOIP-deficient cells transduced with a retroviral vector (pMIP) lacking a cDNA insert (empty vector). HOIP is required for CD40-mediated CD80 upregulation and activation of germline epsilon transcription Engagement of CD40 on B cells upregulates expression of CD80 [10], a cell surface protein that promotes activation of T cells interacting with B cells and other APC. To begin evaluating potential contributions of HOIP to CD40 signaling, we assayed the upregulation of CD80 induced by engagement of CD40 on parental A20.2J, HOIP-deficient, and HOIP-reconstituted cells (Fig. 2). Compared to the parental cell line, the CD40-stimulated upregulation of CD80 was dramatically reduced in all HOIP-deficient cells tested. In contrast, upregulation of CD80 was restored in HOIP-reconstituted cells, confirming that the defect in CD80 upregulation in the HOIP-deficient cells was due to the disruption of Rnf31. 10.1371/journal.pone.0023061.g002 Figure 2 CD40-mediated CD80 upregulation is defective in HOIP-deficient cells. Cells were incubated for 72 hrs with an isotype control antibody (left) or anti-CD40 antibody (right) and then stained for expression of CD80 (filled profiles indicate staining with anti-CD80 antibody; open profiles are staining with an isotype control antibody). Transduction of both HOIP-deficient (HOIP-/-) clones with a retrovirus encoding FLAG-HOIP reversed defective CD80 upregulation, while transduction with an empty vector (pMIP) did not. Similar results were obtained in a second experiment. CD40 signals in B cells can also contribute to the activation of DNA recombination in the immunoglobulin heavy chain locus [11], [12]. This process, known as immunoglobulin class switching (or isotype switching), allows B cells, initially expressing IgM, to switch to IgA, IgG, or IgE depending on the class of antibody most appropriate for a particular immune response. Switching to IgE can be induced by the combination of IL-4 and CD40 signals. The gene rearrangement necessary for IgE production is preceded by the production of non-coding RNA transcripts from germline sequences in the IgE heavy chain locus [13]. To determine whether HOIP is required in the initiation of the class switching process, the relevant B cell lines were stimulated through CD40 in the presence of IL-4 and tested for production of germline epsilon (GLε) transcripts (Fig. 3). Anti-CD40 antibody stimulation alone or together with IL-4 induced GLε transcription in parental A20.2J cells as expected. In contrast, only low levels of GLε expression were detected in HOIP-deficient cells stimulated with either anti-CD40 antibody alone or anti-CD40 and IL-4. Normal levels of GLε expression were induced in HOIP-reconstituted cells stimulated with anti-CD40 antibody alone or together with IL-4. Collectively, these data demonstrate that HOIP has a critical role in cellular responses to CD40 signaling. 10.1371/journal.pone.0023061.g003 Figure 3 GLε transcription is defective in CD40-stimulated HOIP-deficient cells. A20.2J and HOIP-deficient cells transduced with an empty retroviral vector (A20.2J + pMIP and HOIP-/- + pMIP, respectively), or HOIP-deficient cells transduced with a HOIP-encoding retrovirus (HOIP-/- + HOIP) were cultured overnight with agonistic anti-CD40 or an isotype control antibody (iso), with or without IL-4. RNA was isolated from the cells, reverse-transcribed, and then subjected to quantitative PCR to determine levels of GLε transcripts. Results were normalized to the levels of Hprt1 transcripts in each sample. Symbols indicate the values from duplicate cultures (a line indicates the mean of the two values). Similar results were obtained in a second experiment and in an additional experiment with a second HOIP-deficient clone. HOIP mediates CD40-stimulated NF-κB and JNK activation The results described above show that HOIP plays an important role in CD40-mediated effector functions of B cells. It follows that HOIP is likely a key mediator of CD40 signaling. To test this hypothesis, we stimulated A20.2J and HOIP-deficient cells with CD154 (CD40 ligand) expressed by HI5 insect cells [7], [8] and measured activation of the NF-κB and JNK pathways, two of the major transcriptional regulators activated by CD40 [4]. Cell-associated CD154 was used as the stimulus in these experiments as it typically provides more robust, and therefore more readily detected, activation signals than does anti-CD40 antibody. Activation of the canonical NF-κB pathway is initiated with the phosphorylation of IκB proteins by the IκB kinase complex (IKK). In resting cells, IκB proteins are responsible for sequestering NF-κB subunits in the cytoplasm. Phosphorylation by the IKK"
R5843 T7485 T7484 arg2Of CD40,with
R5844 T7488 T7486 arg1Of defects,The
R5845 T7488 T7487 arg1Of defects,marked
R5846 T7488 T7489 arg1Of defects,in
R5847 T7488 T7495 arg2Of defects,displayed
R5848 T7488 T7499 arg1Of defects,suggested
R5849 T7492 T7493 arg1Of activation,and
R585 T665 T664 arg2Of undetectable,was
R5850 T7493 T7489 arg2Of and,in
R5851 T7493 T7490 arg1Of and,CD40-mediated
R5852 T7493 T7491 arg1Of and,cell
R5853 T7494 T7493 arg2Of signaling,and
R5854 T7498 T7495 arg1Of cells,displayed
R5855 T7498 T7496 arg2Of cells,by
R5856 T7498 T7497 arg1Of cells,HOIP-deficient
R5857 T7501 T7502 arg1Of HOIP,mediates
R5858 T7502 T7499 arg2Of mediates,suggested
R5859 T7502 T7500 arg1Of mediates,that
R586 T668 T666 arg2Of cells,in
R5860 T7503 T7502 arg2Of recruitment,mediates
R5861 T7503 T7504 arg1Of recruitment,of
R5862 T7503 T7512 arg1Of recruitment,to
R5863 T7506 T7504 arg2Of components,of
R5864 T7506 T7505 arg1Of components,critical
R5865 T7506 T7507 arg1Of components,of
R5866 T7511 T7507 arg2Of apparatus,of
R5867 T7511 T7508 arg1Of apparatus,the
R5868 T7511 T7509 arg1Of apparatus,CD40
R5869 T7511 T7510 arg1Of apparatus,signaling
R587 T668 T667 arg1Of cells,HOIP-deficient
R5870 T7514 T7512 arg2Of receptor,to
R5871 T7514 T7513 arg1Of receptor,the
R5872 T7517 T7518 arg1Of we,demonstrated
R5873 T7518 T7515 arg1Of demonstrated,Previously
R5874 T7518 T7516 arg1Of demonstrated,","
R5875 T7520 T7521 arg1Of HOIP,is
R5876 T7520 T7522 arg2Of HOIP,recruited
R5877 T7522 T7518 arg2Of recruited,demonstrated
R5878 T7522 T7519 arg1Of recruited,that
R5879 T7522 T7521 arg2Of recruited,is
R588 T671 T664 arg3Of explaining,was
R5880 T7522 T7523 arg1Of recruited,to
R5881 T7522 T7528 arg1Of recruited,in
R5882 T7522 T7532 arg1Of recruited,","
R5883 T7522 T7533 modOf recruited,suggesting
R5884 T7522 T7540 arg1Of recruited,[
R5885 T7527 T7523 arg2Of complex,to
R5886 T7527 T7524 arg1Of complex,the
R5887 T7527 T7525 arg1Of complex,CD40
R5888 T7527 T7526 arg1Of complex,signaling
R5889 T7531 T7528 arg2Of manner,in
R589 T671 T670 arg1Of explaining,potentially
R5890 T7531 T7529 arg1Of manner,a
R5891 T7531 T7530 arg1Of manner,TRAF2-dependent
R5892 T7536 T7533 arg2Of functions,suggesting
R5893 T7536 T7534 arg1Of functions,that
R5894 T7536 T7535 arg1Of functions,HOIP
R5895 T7536 T7537 arg1Of functions,downstream
R5896 T7537 T7538 arg1Of downstream,of
R5897 T7539 T7538 arg2Of TRAF2,of
R5898 T7541 T7540 arg2Of 6,[
R5899 T7542 T7540 arg3Of ],[
R590 T676 T674 arg2Of activation,in
R5900 T7543 T7544 arg1Of Studies,by
R5901 T7543 T7546 arg1Of Studies,suggest
R5902 T7545 T7544 arg2Of others,by
R5903 T7550 T7548 arg1Of proteins,the
R5904 T7550 T7549 arg1Of proteins,TRAF2-associated
R5905 T7551 T7552 arg1Of cIAP1,and
R5906 T7552 T7550 arg1Of and,proteins
R5907 T7552 T7554 arg1Of and,play
R5908 T7553 T7552 arg2Of cIAP2,and
R5909 T7554 T7546 arg2Of play,suggest
R591 T676 T675 arg1Of activation,NF-κB
R5910 T7554 T7547 arg1Of play,that
R5911 T7554 T7557 arg1Of play,in
R5912 T7554 T7566 arg1Of play,[
R5913 T7556 T7554 arg2Of role,play
R5914 T7556 T7555 arg1Of role,a
R5915 T7559 T7557 arg2Of recruitment,in
R5916 T7559 T7558 arg1Of recruitment,the
R5917 T7559 T7560 arg1Of recruitment,of
R5918 T7559 T7562 arg1Of recruitment,to
R5919 T7561 T7560 arg2Of HOIP,of
R592 T677 T678 arg1Of Restoration,of
R5920 T7563 T7564 arg1Of TNFR1,and
R5921 T7564 T7562 arg2Of and,to
R5922 T7565 T7564 arg2Of CD40,and
R5923 T7567 T7566 arg2Of 14,[
R5924 T7568 T7566 arg3Of ],[
R5925 T7571 T7572 arg1Of we,determined
R5926 T7572 T7569 arg1Of determined,Therefore
R5927 T7572 T7570 arg1Of determined,","
R5928 T7575 T7574 arg1Of association,the
R5929 T7575 T7576 arg1Of association,of
R593 T677 T681 arg1Of Restoration,reversed
R5930 T7575 T7578 arg1Of association,with
R5931 T7575 T7580 arg1Of association,in
R5932 T7575 T7583 arg1Of association,was
R5933 T7575 T7584 arg2Of association,altered
R5934 T7577 T7576 arg2Of HOIP,of
R5935 T7579 T7578 arg2Of CD40,with
R5936 T7582 T7580 arg2Of cells,in
R5937 T7582 T7581 arg1Of cells,A20.2J
R5938 T7584 T7572 arg2Of altered,determined
R5939 T7584 T7573 arg1Of altered,whether
R594 T680 T678 arg2Of expression,of
R5940 T7584 T7583 arg2Of altered,was
R5941 T7584 T7605 arg1Of altered,[
R5942 T7586 T7584 arg1Of treatment,altered
R5943 T7586 T7585 arg2Of treatment,by
R5944 T7586 T7587 arg1Of treatment,of
R5945 T7586 T7589 arg1Of treatment,with
R5946 T7586 T7599 arg2Of treatment,derived
R5947 T7588 T7587 arg2Of cells,of
R5948 T7591 T7589 arg2Of inhibitor,with
R5949 T7591 T7590 arg1Of inhibitor,an
R595 T680 T679 arg1Of expression,HOIP
R5950 T7591 T7592 arg1Of inhibitor,of
R5951 T7591 T7595 arg1Of inhibitor,","
R5952 T7594 T7592 arg2Of activity,of
R5953 T7594 T7593 arg1Of activity,cIAP
R5954 T7598 T7595 arg2Of peptide,","
R5955 T7598 T7596 arg1Of peptide,a
R5956 T7598 T7597 arg1Of peptide,membrane-permeable
R5957 T7599 T7600 arg1Of derived,from
R5958 T7604 T7600 arg2Of SMAC,from
R5959 T7604 T7601 arg1Of SMAC,the
R596 T683 T681 arg2Of defects,reversed
R5960 T7604 T7602 arg1Of SMAC,apoptosis
R5961 T7604 T7603 arg1Of SMAC,regulator
R5962 T7606 T7605 arg2Of 15,[
R5963 T7607 T7605 arg3Of ],[
R5964 T7608 T7609 arg1Of We,found
R5965 T7611 T7612 arg1Of pretreatment,of
R5966 T7611 T7614 arg1Of pretreatment,with
R5967 T7611 T7619 arg1Of pretreatment,reduced
R5968 T7613 T7612 arg2Of cells,of
R5969 T7617 T7614 arg2Of peptide,with
R597 T683 T682 arg1Of defects,the
R5970 T7617 T7615 arg1Of peptide,the
R5971 T7617 T7616 arg1Of peptide,SMAC
R5972 T7619 T7609 arg2Of reduced,found
R5973 T7619 T7610 arg1Of reduced,that
R5974 T7619 T7618 arg1Of reduced,dramatically
R5975 T7621 T7619 arg2Of amount,reduced
R5976 T7621 T7620 arg1Of amount,the
R5977 T7621 T7622 arg1Of amount,of
R5978 T7621 T7624 arg2Of amount,associated
R5979 T7623 T7622 arg2Of cIAP1,of
R598 T683 T684 arg1Of defects,in
R5980 T7624 T7625 arg1Of associated,with
R5981 T7629 T7625 arg2Of complex,with
R5982 T7629 T7626 arg1Of complex,the
R5983 T7629 T7627 arg1Of complex,CD40
R5984 T7629 T7628 arg1Of complex,signaling
R5985 T7629 T7630 arg1Of complex,in
R5986 T7631 T7630 arg2Of cells,in
R5987 T7631 T7632 arg2Of cells,stimulated
R5988 T7632 T7633 arg1Of stimulated,with
R5989 T7636 T7633 arg2Of beads,with
R599 T686 T687 arg1Of activation,and
R5990 T7636 T7634 arg1Of beads,anti-CD40
R5991 T7636 T7635 arg1Of beads,antibody-coated
R5992 T7636 T7637 arg1Of beads,(
R5993 T7639 T7637 arg2Of 5A,(
R5994 T7639 T7638 arg1Of 5A,Fig.
R5995 T7640 T7637 arg3Of ),(
R5996 T7643 T7641 arg1Of treatment,SMAC
R5997 T7643 T7642 arg1Of treatment,peptide
R5998 T7643 T7645 arg1Of treatment,resulted
R5999 T7645 T7644 arg1Of resulted,also
R600 T687 T684 arg2Of and,in
R6000 T7645 T7646 arg1Of resulted,in
R6001 T7648 T7649 arg1Of slight,but
R6002 T7650 T7649 arg2Of reproducible,but
R6003 T7651 T7646 arg2Of decrease,in
R6004 T7651 T7647 arg1Of decrease,a
R6005 T7651 T7648 arg1Of decrease,slight
R6006 T7651 T7650 arg1Of decrease,reproducible
R6007 T7651 T7652 arg1Of decrease,in
R6008 T7651 T7664 arg1Of decrease,","
R6009 T7651 T7665 arg1Of decrease,along
R601 T687 T685 arg1Of and,cellular
R6010 T7654 T7652 arg2Of amount,in
R6011 T7654 T7653 arg1Of amount,the
R6012 T7654 T7655 arg1Of amount,of
R6013 T7659 T7655 arg2Of form,of
R6014 T7659 T7656 arg1Of form,the
R6015 T7659 T7657 arg1Of form,major
R6016 T7659 T7658 arg1Of form,HOIP
R6017 T7659 T7660 arg2Of form,recovered
R6018 T7663 T7660 arg1Of immunoprecipitation,recovered
R6019 T7663 T7661 arg2Of immunoprecipitation,by
R602 T688 T687 arg2Of signaling,and
R6020 T7663 T7662 arg1Of immunoprecipitation,CD40
R6021 T7665 T7666 arg1Of along,with
R6022 T7669 T7666 arg2Of increase,with
R6023 T7669 T7667 arg1Of increase,an
R6024 T7669 T7668 arg1Of increase,apparent
R6025 T7669 T7670 arg1Of increase,in
R6026 T7674 T7670 arg2Of species,in
R6027 T7674 T7671 arg1Of species,higher
R6028 T7674 T7672 arg1Of species,molecular
R6029 T7674 T7673 arg1Of species,weight
R603 T690 T689 arg1Of results,These
R6030 T7674 T7675 arg2Of species,recognized
R6031 T7678 T7675 arg1Of antibody,recognized
R6032 T7678 T7676 arg2Of antibody,by
R6033 T7678 T7677 arg1Of antibody,anti-HOIP
R6034 T7680 T7679 arg2Of contrast,In
R6035 T7682 T7683 arg1Of treatment,with
R6036 T7682 T7687 arg1Of treatment,did
R6037 T7682 T7689 arg1Of treatment,alter
R6038 T7686 T7683 arg2Of peptide,with
R6039 T7686 T7684 arg1Of peptide,the
R604 T690 T691 arg1Of results,reveal
R6040 T7686 T7685 arg1Of peptide,SMAC
R6041 T7689 T7679 arg1Of alter,In
R6042 T7689 T7681 arg1Of alter,","
R6043 T7689 T7687 arg2Of alter,did
R6044 T7689 T7688 arg1Of alter,not
R6045 T7691 T7692 arg1Of amount,or
R6046 T7692 T7689 arg2Of or,alter
R6047 T7692 T7690 arg1Of or,the
R6048 T7692 T7695 arg1Of or,of
R6049 T7692 T7697 arg1Of or,present
R605 T691 T693 arg1Of reveal,as
R6050 T7694 T7692 arg2Of weight,or
R6051 T7694 T7693 arg1Of weight,molecular
R6052 T7696 T7695 arg2Of HOIP,of
R6053 T7697 T7698 arg1Of present,in
R6054 T7700 T7698 arg2Of lysates,in
R6055 T7700 T7699 arg1Of lysates,cell
R6056 T7702 T7701 arg1Of data,These
R6057 T7702 T7703 arg1Of data,suggest
R6058 T7707 T7705 arg1Of treatment,SMAC
R6059 T7707 T7706 arg1Of treatment,peptide
R606 T692 T691 arg2Of HOIP,reveal
R6060 T7707 T7709 arg1Of treatment,alters
R6061 T7709 T7703 arg2Of alters,suggest
R6062 T7709 T7704 arg1Of alters,that
R6063 T7709 T7708 arg1Of alters,specifically
R6064 T7711 T7710 arg1Of characteristics,the
R6065 T7711 T7712 arg1Of characteristics,of
R6066 T7711 T7716 arg1Of characteristics,than
R6067 T7714 T7712 arg2Of HOIP,of
R6068 T7714 T7713 arg1Of HOIP,CD40-associated
R6069 T7716 T7709 arg2Of than,alters
R607 T696 T693 arg2Of component,as
R6070 T7716 T7715 arg1Of than,rather
R6071 T7720 T7716 arg2Of pool,than
R6072 T7720 T7717 arg1Of pool,the
R6073 T7720 T7718 arg1Of pool,entire
R6074 T7720 T7719 arg1Of pool,cellular
R6075 T7720 T7721 arg1Of pool,of
R6076 T7723 T7721 arg2Of protein,of
R6077 T7723 T7722 arg1Of protein,this
R6078 T7727 T7726 arg1Of results,these
R6079 T7727 T7728 arg1Of results,support
R608 T696 T694 arg1Of component,a
R6080 T7728 T7724 arg1Of support,Together
R6081 T7728 T7725 arg1Of support,","
R6082 T7730 T7728 arg2Of idea,support
R6083 T7730 T7729 arg1Of idea,the
R6084 T7734 T7732 arg1Of proteins,the
R6085 T7734 T7733 arg1Of proteins,cIAP
R6086 T7734 T7735 arg1Of proteins,influence
R6087 T7735 T7730 arg2Of influence,idea
R6088 T7735 T7731 arg1Of influence,that
R6089 T7737 T7736 arg1Of recruitment,the
R609 T696 T695 arg1Of component,key
R6090 T7737 T7738 arg1Of recruitment,and
R6091 T7738 T7735 arg2Of and,influence
R6092 T7738 T7742 arg1Of and,of
R6093 T7741 T7738 arg2Of state,and
R6094 T7741 T7739 arg1Of state,post-translational
R6095 T7741 T7740 arg1Of state,modification
R6096 T7744 T7742 arg2Of HOIP,of
R6097 T7744 T7743 arg1Of HOIP,CD40-associated
R6098 T7746 T7745 arg1Of test,To
R6099 T7748 T7746 arg2Of possibility,test
R610 T696 T697 arg1Of component,of
R6100 T7748 T7747 arg1Of possibility,the
R6101 T7750 T7751 arg1Of HOIP,is
R6102 T7750 T7752 arg1Of HOIP,responsible
R6103 T7751 T7748 arg2Of is,possibility
R6104 T7751 T7749 arg1Of is,that
R6105 T7752 T7751 arg2Of responsible,is
R6106 T7752 T7753 arg1Of responsible,for
R6107 T7755 T7753 arg2Of recruitment,for
R6108 T7755 T7754 arg1Of recruitment,the
R6109 T7755 T7756 arg1Of recruitment,of
R611 T701 T697 arg2Of pathway,of
R6110 T7755 T7761 arg1Of recruitment,to
R6111 T7760 T7756 arg2Of proteins,of
R6112 T7760 T7757 arg1Of proteins,other
R6113 T7760 T7758 arg1Of proteins,critical
R6114 T7760 T7759 arg1Of proteins,signaling
R6115 T7762 T7761 arg2Of CD40,to
R6116 T7764 T7746 arg1Of we,test
R6117 T7764 T7765 arg1Of we,immunoprecipitated
R6118 T7765 T7745 modOf immunoprecipitated,To
R6119 T7765 T7763 arg1Of immunoprecipitated,","
R612 T701 T698 arg1Of pathway,the
R6120 T7768 T7765 arg2Of complexes,immunoprecipitated
R6121 T7768 T7766 arg1Of complexes,CD40
R6122 T7768 T7767 arg1Of complexes,signaling
R6123 T7768 T7769 arg1Of complexes,from
R6124 T7770 T7771 arg1Of HOIP-deficient,and
R6125 T7772 T7771 arg2Of HOIP-reconstituted,and
R6126 T7773 T7769 arg2Of cells,from
R6127 T7773 T7770 arg1Of cells,HOIP-deficient
R6128 T7773 T7772 arg1Of cells,HOIP-reconstituted
R6129 T7773 T7774 arg1Of cells,(
R613 T701 T699 arg1Of pathway,CD40
R6130 T7776 T7774 arg2Of 5B,(
R6131 T7776 T7775 arg1Of 5B,Fig.
R6132 T7777 T7774 arg3Of ),(
R6133 T7779 T7778 arg2Of described,As
R6134 T7779 T7780 arg1Of described,previously
R6135 T7784 T7782 arg1Of complexes,CD40
R6136 T7784 T7783 arg1Of complexes,signaling
R6137 T7784 T7785 arg2Of complexes,immunoprecipitated
R6138 T7784 T7789 arg1Of complexes,contained
R6139 T7785 T7786 arg1Of immunoprecipitated,from
R614 T701 T700 arg1Of pathway,signaling
R6140 T7788 T7786 arg2Of cells,from
R6141 T7788 T7787 arg1Of cells,A20.2J
R6142 T7789 T7778 arg1Of contained,As
R6143 T7789 T7781 arg1Of contained,","
R6144 T7790 T7793 arg1Of HOIP,as
R6145 T7793 T7789 arg2Of as,contained
R6146 T7793 T7791 arg1Of as,as
R6147 T7793 T7792 arg1Of as,well
R6148 T7794 T7795 arg1Of TRAF2,","
R6149 T7795 T7797 arg1Of ",",","
R6150 T7796 T7795 arg2Of TRAF3,","
R6151 T7797 T7800 arg1Of ",",and
R6152 T7798 T7797 arg2Of IKKα/β,","
R6153 T7800 T7793 arg2Of and,as
R6154 T7800 T7799 arg1Of and,","
R6155 T7801 T7800 arg2Of IKKγ,and
R6156 T7801 T7802 arg1Of IKKγ,[
R6157 T7803 T7802 arg2Of 6,[
R6158 T7804 T7802 arg3Of ],[
R6159 T7806 T7805 arg1Of amounts,The
R6160 T7861 T7859 arg2Of 5B,(
R6161 T7861 T7860 arg1Of 5B,Fig.
R6162 T7861 T7862 arg1Of 5B,","
R6163 T7806 T7807 arg1Of amounts,of
R6164 T7806 T7814 arg1Of amounts,in
R6165 T7863 T7862 arg2Of C,","
R6166 T7864 T7859 arg3Of ),(
R6167 T7806 T7820 arg1Of amounts,were
R6168 T7866 T7865 arg1Of amounts,The
R6169 T7866 T7867 arg1Of amounts,of
R6170 T7866 T7871 arg1Of amounts,in
R6171 T7866 T7877 arg1Of amounts,were
R6172 T7866 T7878 arg1Of amounts,similar
R6173 T7866 T7881 arg2Of amounts,detected
R6174 T7868 T7869 arg1Of IKKα/β,and
R6175 T7806 T7821 arg1Of amounts,similar
R6176 T7869 T7867 arg2Of and,of
R6177 T7808 T7809 arg1Of TRAF2,","
R6178 T7870 T7869 arg2Of IKKγ,and
R6179 T7873 T7871 arg2Of immunoprecipitates,in
R6180 T7873 T7872 arg1Of immunoprecipitates,CD40
R6181 T7873 T7874 arg1Of immunoprecipitates,from
R6182 T7809 T7812 arg1Of ",",and
R6183 T7876 T7874 arg2Of cells,from
R6184 T7876 T7875 arg1Of cells,HOIP-reconstituted
R6185 T7810 T7809 arg2Of TRAF3,","
R6186 T7878 T7879 arg1Of similar,to
R6187 T7880 T7879 arg2Of those,to
R6188 T7812 T7807 arg2Of and,of
R6189 T7881 T7877 arg2Of detected,were
R6190 T7881 T7878 modOf detected,similar
R6191 T7881 T7882 arg1Of detected,in
R6192 T7881 T7888 arg1Of detected,","
R6193 T7883 T7882 arg2Of samples,in
R6194 T7883 T7884 arg2Of samples,prepared
R6195 T7884 T7885 arg1Of prepared,from
R6196 T7812 T7811 arg1Of and,","
R6197 T7813 T7812 arg2Of cIAP1,and
R6198 T7887 T7885 arg2Of cells,from
R6199 T7887 T7886 arg1Of cells,parental
R6200 T7889 T7881 arg3Of demonstrating,detected
R6201 T7816 T7814 arg2Of immunoprecipitates,in
R6202 T7892 T7891 arg1Of defects,the
R6203 T7892 T7893 arg1Of defects,in
R6204 T7892 T7898 arg1Of defects,were
R6205 T7892 T7899 arg1Of defects,due
R6206 T7895 T7893 arg2Of recruitment,in
R6207 T7895 T7894 arg1Of recruitment,IKK
R6208 T7895 T7897 arg2Of recruitment,observed
R6209 T7896 T7897 arg1Of we,observed
R6210 T7816 T7815 arg1Of immunoprecipitates,CD40
R6211 T7898 T7889 arg2Of were,demonstrating
R6212 T7898 T7890 arg1Of were,that
R6213 T7816 T7817 arg1Of immunoprecipitates,from
R6214 T7899 T7898 arg2Of due,were
R6215 T7819 T7817 arg2Of cells,from
R6216 T7899 T7901 arg1Of due,to
R6217 T7901 T7900 arg1Of to,specifically
R6218 T7903 T7901 arg2Of absence,to
R6219 T7903 T7902 arg1Of absence,the
R6220 T7903 T7904 arg1Of absence,of
R6221 T7819 T7818 arg1Of cells,HOIP-deficient
R6222 T7820 T7828 arg1Of were,","
R6223 T7906 T7904 arg2Of expression,of
R6224 T7906 T7905 arg1Of expression,HOIP
R6225 T7908 T7907 arg1Of data,These
R6226 T7908 T7909 arg1Of data,indicate
R6227 T7820 T7829 modOf were,indicating
R6228 T7821 T7820 arg2Of similar,were
R6229 T7911 T7912 arg1Of HOIP,is
R6230 T7911 T7913 arg2Of HOIP,required
R6231 T7821 T7822 arg1Of similar,to
R6232 T7913 T7909 arg2Of required,indicate
R6233 T7913 T7910 arg1Of required,that
R6234 T7913 T7912 arg2Of required,is
R6235 T7913 T7914 arg1Of required,for
R6236 T7823 T7822 arg2Of those,to
R6237 T7915 T7914 arg2Of recruitment,for
R6238 T7915 T7916 arg1Of recruitment,of
R6239 T7915 T7920 arg1Of recruitment,to
R6240 T7823 T7824 arg1Of those,from
R6241 T7919 T7916 arg2Of complex,of
R6242 T7919 T7917 arg1Of complex,the
R6243 T7919 T7918 arg1Of complex,NF-κB-activating
R6244 T7827 T7824 arg2Of cells,from
R6245 T7924 T7920 arg2Of complex,to
R6246 T7924 T7921 arg1Of complex,the
R6247 T7924 T7922 arg1Of complex,CD40
R6248 T7924 T7923 arg1Of complex,signaling
R6249 T7926 T7925 arg2Of shown,As
R6250 T7827 T7825 arg1Of cells,parental
R6251 T7827 T7826 arg1Of cells,A20.2J
R6252 T7831 T7832 arg1Of HOIP,is
R6253 T7926 T7927 arg1Of shown,here
R6254 T7926 T7929 arg1Of shown,in
R6255 T7926 T7933 arg1Of shown,[
R6256 T7927 T7928 arg1Of here,and
R6257 T7929 T7928 arg2Of in,and
R6258 T7831 T7834 arg2Of HOIP,required
R6259 T7932 T7929 arg2Of study,in
R6260 T7932 T7930 arg1Of study,our
R6261 T7932 T7931 arg1Of study,previous
R6262 T7834 T7829 arg2Of required,indicating
R6263 T7934 T7933 arg2Of 6,[
R6264 T7935 T7933 arg3Of ],[
R6265 T7937 T7938 arg1Of IKKγ,present
R6266 T7937 T7942 arg1Of IKKγ,appears
R6267 T7937 T7944 arg1Of IKKγ,have
R6268 T7938 T7939 arg1Of present,in
R6269 T7941 T7939 arg2Of immunoprecipitates,in
R6270 T7941 T7940 arg1Of immunoprecipitates,CD40
R6271 T7834 T7830 arg1Of required,that
R6272 T7942 T7925 arg1Of appears,As
R6273 T7942 T7936 arg1Of appears,","
R6274 T7834 T7832 arg2Of required,is
R6275 T7944 T7942 arg2Of have,appears
R6276 T7944 T7943 arg1Of have,to
R6277 T7944 T7949 arg1Of have,than
R6278 T7834 T7833 arg1Of required,not
R6279 T7834 T7835 arg1Of required,for
R6280 T7837 T7835 arg2Of association,for
R6281 T7948 T7944 arg2Of weight,have
R6282 T7948 T7945 arg1Of weight,a
R6283 T7948 T7946 arg1Of weight,higher
R6284 T7948 T7947 arg1Of weight,molecular
R6285 T7950 T7949 arg2Of that,than
R6286 T7950 T7951 arg2Of that,found
R6287 T7951 T7952 arg1Of found,in
R6288 T7837 T7836 arg1Of association,the
R6289 T7954 T7952 arg2Of lysates,in
R6290 T7954 T7953 arg1Of lysates,cell
R6291 T7837 T7838 arg1Of association,of
R6292 T7956 T7955 arg1Of confirm,To
R6293 T7837 T7841 arg1Of association,with
R6294 T7840 T7838 arg2Of proteins,of
R6295 T7840 T7839 arg1Of proteins,these
R6296 T7842 T7841 arg2Of CD40,with
R6297 T7844 T7843 arg2Of contrast,In
R6298 T7846 T7847 arg1Of IKKα/β,and
R6299 T7962 T7958 arg1Of species,this
R6300 T7962 T7959 arg1Of species,higher
R6301 T7962 T7960 arg1Of species,molecular
R6302 T7962 T7961 arg1Of species,weight
R6303 T7962 T7963 arg1Of species,was
R6304 T7963 T7956 arg2Of was,confirm
R6305 T7963 T7957 arg1Of was,that
R6306 T7963 T7964 arg1Of was,indeed
R6307 T7965 T7963 arg2Of IKKγ,was
R6308 T7847 T7849 arg1Of and,were
R6309 T7967 T7956 arg1Of we,confirm
R6310 T7967 T7968 arg1Of we,generated
R6311 T7968 T7955 modOf generated,To
R6312 T7968 T7966 arg1Of generated,","
R6313 T7847 T7851 arg1Of and,detectable
R6314 T7971 T7968 arg2Of lines,generated
R6315 T7971 T7969 arg1Of lines,A20.2J
R6316 T7971 T7970 arg1Of lines,cell
R6317 T7971 T7972 arg1Of lines,containing
R6318 T7848 T7847 arg2Of IKKγ,and
R6319 T7975 T7974 arg1Of integrated,stably
R6320 T7849 T7843 arg1Of were,In
R6321 T7977 T7972 arg2Of vector,containing
R6322 T7977 T7973 arg1Of vector,a
R6323 T7977 T7975 arg1Of vector,integrated
R6324 T7977 T7976 arg1Of vector,retroviral
R6325 T7977 T7978 arg1Of vector,that
R6326 T7977 T7979 arg1Of vector,encoded
R6327 T7849 T7845 arg1Of were,","
R6328 T7849 T7850 arg1Of were,not
R6329 T7849 T7852 arg1Of were,in
R6330 T7982 T7979 arg2Of version,encoded
R6331 T7982 T7980 arg1Of version,an
R6332 T7982 T7981 arg1Of version,epitope-tagged
R6333 T7982 T7983 arg1Of version,of
R6334 T7851 T7849 arg2Of detectable,were
R6335 T7985 T7983 arg2Of IKKγ,of
R6336 T7985 T7984 arg1Of IKKγ,mouse
R6337 T7854 T7852 arg2Of immunoprecipitates,in
R6338 T7987 T7986 arg1Of lysates,Cell
R6339 T7987 T7988 arg1Of lysates,and
R6340 T7988 T7995 arg1Of and,were
R6341 T7988 T7996 arg2Of and,analyzed
R6342 T7990 T7988 arg2Of immunoprecipitates,and
R6343 T7990 T7989 arg1Of immunoprecipitates,CD40
R6344 T7990 T7991 arg2Of immunoprecipitates,prepared
R6345 T7991 T7992 arg1Of prepared,from
R6346 T7854 T7853 arg1Of immunoprecipitates,CD40
R6347 T7854 T7855 arg2Of immunoprecipitates,recovered
R6348 T7994 T7992 arg2Of cells,from
R6349 T7994 T7993 arg1Of cells,these
R6350 T7855 T7856 arg1Of recovered,from
R6351 T7996 T7995 arg2Of analyzed,were
R6352 T7996 T7997 arg1Of analyzed,by
R6353 T7858 T7856 arg2Of cells,from
R6354 T7999 T7997 arg2Of blotting,by
R6355 T7999 T7998 arg1Of blotting,Western
R6356 T7999 T8000 arg1Of blotting,for
R6357 T8003 T8000 arg2Of tag,for
R6358 T8003 T8001 arg1Of tag,the
R6359 T8003 T8002 arg1Of tag,epitope
R6360 T8003 T8004 arg1Of tag,(
R6361 T7858 T7857 arg1Of cells,HOIP-deficient
R6362 T7858 T7859 arg1Of cells,(
R6363 T8006 T8004 arg2Of 5C,(
R6364 T8006 T8005 arg1Of 5C,Fig.
R6365 T8054 T8053 arg2Of CD40,to
R6366 T8007 T8004 arg3Of ),(
R6367 T8009 T8008 arg1Of analysis,This
R6368 T8009 T8010 arg1Of analysis,produced
R6369 T8012 T8010 arg2Of pattern,produced
R6370 T8012 T8011 arg1Of pattern,a
R6371 T8012 T8013 arg1Of pattern,of
R6372 T8012 T8015 arg1Of pattern,that
R6373 T8012 T8016 arg1Of pattern,was
R6374 T8014 T8013 arg2Of bands,of
R6375 T8016 T8017 arg1Of was,essentially
R6376 T8016 T8023 modOf was,using
R6377 T8055 T8048 arg2Of requires,confirmed
R6378 T8019 T8016 arg2Of same,was
R6379 T8019 T8018 arg1Of same,the
R6380 T8019 T8020 arg1Of same,as
R6381 T8019 T8022 arg2Of same,obtained
R6382 T8021 T8020 arg2Of that,as
R6383 T8055 T8049 arg1Of requires,that
R6384 T8056 T8055 arg2Of HOIP,requires
R6385 T8025 T8023 arg2Of antibody,using
R6386 T8025 T8024 arg1Of antibody,an
R6387 T8025 T8026 arg1Of antibody,specific
R6388 T8026 T8027 arg1Of specific,for
R6389 T8058 T8057 arg1Of determine,To
R6390 T8029 T8027 arg2Of IKKγ,for
R6391 T8029 T8028 arg1Of IKKγ,native
R6392 T8029 T8030 arg1Of IKKγ,(
R6393 T8059 T8058 arg2Of what,determine
R6394 T8033 T8034 arg1Of B,and
R6395 T8034 T8030 arg2Of and,(
R6396 T8034 T8031 arg1Of and,compare
R6397 T8034 T8032 arg1Of and,panels
R6398 T8034 T8036 arg1Of and,in
R6399 T8035 T8034 arg2Of C,and
R6400 T8037 T8036 arg2Of Fig.,in
R6401 T8037 T8038 arg1Of Fig.,5
R6402 T8059 T8060 arg1Of what,was
R6403 T8039 T8030 arg3Of ),(
R6404 T8059 T8061 arg1Of what,responsible
R6405 T8061 T8060 arg2Of responsible,was
R6406 T8042 T8043 arg1Of analysis,of
R6407 T8042 T8048 arg1Of analysis,confirmed
R6408 T8044 T8043 arg2Of cells,of
R6409 T8044 T8045 arg1Of cells,expressing
R6410 T8061 T8062 arg1Of responsible,for
R6411 T8047 T8045 arg2Of tagged-IKKγ,expressing
R6412 T8047 T8046 arg1Of tagged-IKKγ,epitope
R6413 T8066 T8062 arg2Of weight,for
R6414 T8048 T8040 arg1Of confirmed,Moreover
R6415 T8048 T8041 arg1Of confirmed,","
R6416 T8050 T8051 arg1Of recruitment,of
R6417 T8050 T8053 arg1Of recruitment,to
R6418 T8050 T8055 arg1Of recruitment,requires
R6419 T8052 T8051 arg2Of IKKγ,of
R6420 T8066 T8063 arg1Of weight,the
R6421 T8066 T8064 arg2Of weight,increased
R6422 T8066 T8065 arg1Of weight,molecular
R6423 T8066 T8067 arg1Of weight,of
R6424 T8066 T8069 arg1Of weight,in
R6425 T8068 T8067 arg2Of IKKγ,of
R6426 T8071 T8069 arg2Of immunoprecipitates,in
R6427 T8152 T8149 arg3Of phosphorylation,in
R6428 T8071 T8070 arg1Of immunoprecipitates,CD40
R6429 T8156 T8155 arg1Of data,these
R6430 T8156 T8157 arg1Of data,demonstrate
R6431 T8156 T8171 arg1Of data,suggest
R6432 T8157 T8170 arg1Of demonstrate,and
R6433 T8075 T8073 arg1Of samples,the
R6434 T8159 T8160 arg1Of HOIP,is
R6435 T8159 T8161 arg2Of HOIP,required
R6436 T8161 T8157 arg2Of required,demonstrate
R6437 T8161 T8158 arg1Of required,that
R6438 T8161 T8160 arg2Of required,is
R6439 T8161 T8162 arg1Of required,for
R6440 T8164 T8162 arg2Of association,for
R6441 T8164 T8163 arg1Of association,the
R6442 T8164 T8165 arg1Of association,of
R6443 T8164 T8167 arg1Of association,with
R6444 T8166 T8165 arg2Of IKKγ,of
R6445 T8075 T8074 arg1Of samples,protein
R6446 T8075 T8076 arg1Of samples,were
R6447 T8168 T8167 arg2Of CD40,with
R6448 T8075 T8077 arg2Of samples,incubated
R6449 T8077 T8057 modOf incubated,To
R6450 T8170 T8153 arg1Of and,Overall
R6451 T8170 T8154 arg1Of and,","
R6452 T8170 T8169 arg1Of and,","
R6453 T8171 T8170 arg2Of suggest,and
R6454 T8174 T8173 arg1Of event,this
R6455 T8174 T8175 arg1Of event,is
R6456 T8174 T8176 arg2Of event,coupled
R6457 T8077 T8072 arg1Of incubated,","
R6458 T8176 T8171 arg2Of coupled,suggest
R6459 T8176 T8172 arg1Of coupled,that
R6460 T8176 T8175 arg2Of coupled,is
R6461 T8176 T8177 arg1Of coupled,to
R6462 T8077 T8076 arg2Of incubated,were
R6463 T8077 T8078 arg1Of incubated,with
R6464 T8179 T8177 arg2Of modifications,to
R6465 T8179 T8178 arg1Of modifications,post-translational
R6466 T8179 T8180 arg1Of modifications,of
R6467 T8080 T8078 arg2Of phosphatase,with
R6468 T8181 T8180 arg2Of IKKγ,of
R6469 T8080 T8079 arg1Of phosphatase,lambda
R6470 T8080 T8081 arg1Of phosphatase,","
R6471 T8080 T8082 arg1Of phosphatase,which
R6472 T8080 T8083 arg1Of phosphatase,removes
R6473 T8084 T8083 arg2Of phosphates,removes
R6474 T8084 T8085 arg2Of phosphates,attached
R6475 T8085 T8086 arg1Of attached,to
R6477 T8087 T8088 arg1Of tyrosine,","
R6481 T8088 T8091 arg1Of ",",or
R6483 T8089 T8088 arg2Of threonine,","
R6484 T8091 T8086 arg2Of or,to
R6485 T8091 T8090 arg1Of or,","
R6486 T8091 T8094 arg1Of or,in
R6487 T8093 T8091 arg2Of residues,or
R6488 T8093 T8092 arg1Of residues,serine
R6489 T8095 T8094 arg2Of proteins,in
R6490 T8097 T8096 arg1Of treatment,This
R6491 T8097 T8098 arg1Of treatment,reduced
R6492 T8099 T8098 arg2Of much,reduced
R6493 T8099 T8100 arg1Of much,of
R6494 T8102 T8100 arg2Of IKKγ,of
R6495 T8102 T8101 arg1Of IKKγ,CD40-associated
R6496 T8102 T8103 arg1Of IKKγ,to
R6497 T8107 T8103 arg2Of weight,to
R6498 T8107 T8104 arg1Of weight,an
R6499 T8107 T8105 arg1Of weight,apparent
R6500 T8107 T8106 arg1Of weight,molecular
R6501 T8107 T8108 arg1Of weight,similar
R6502 T8108 T8109 arg1Of similar,to
R6503 T8108 T8114 arg1Of similar,in
R6504 T8110 T8109 arg2Of that,to
R6505 T8110 T8111 arg1Of that,of
R6506 T8113 T8111 arg2Of protein,of
R6507 T8113 T8112 arg1Of protein,the
R6508 T8116 T8114 arg2Of lysates,in
R6509 T8116 T8115 arg1Of lysates,cell
R6510 T8116 T8117 arg1Of lysates,(
R6511 T8119 T8117 arg2Of 5C,(
R6512 T8119 T8118 arg1Of 5C,Fig.
R6513 T8120 T8117 arg3Of ),(
R6514 T8125 T8123 arg1Of one,at
R6515 T8125 T8124 arg1Of one,least
R6516 T8126 T8125 arg1Of band,one
R6517 T8126 T8127 arg1Of band,of
R6518 T8126 T8132 arg1Of band,remained
R6519 T8126 T8133 arg1Of band,after
R6520 T8129 T8128 arg1Of higher,significantly
R6521 T8131 T8127 arg2Of weight,of
R6522 T8131 T8129 arg1Of weight,higher
R6523 T8131 T8130 arg1Of weight,molecular
R6524 T8132 T8121 arg1Of remained,However
R6525 T8132 T8122 arg1Of remained,","
R6526 T8132 T8136 arg1Of remained,","
R6527 T8132 T8137 modOf remained,suggesting
R6528 T8133 T8132 arg2Of after,remained
R6529 T8135 T8133 arg2Of treatment,after
R6530 T8135 T8134 arg1Of treatment,phosphatase
R6531 T8140 T8139 arg1Of IKKγ,CD40-associated
R6532 T8140 T8141 arg1Of IKKγ,is
R6533 T8140 T8142 arg1Of IKKγ,subject
R6534 T8141 T8137 arg2Of is,suggesting
R6535 T8141 T8138 arg1Of is,that
R6536 T8142 T8141 arg2Of subject,is
R6537 T8142 T8143 arg1Of subject,to
R6538 T8146 T8144 arg1Of one,at
R6539 T8146 T8145 arg1Of one,least
R6540 T8148 T8143 arg2Of modification,to
R6541 T8148 T8146 arg1Of modification,one
R6542 T8148 T8147 arg1Of modification,other
R6543 T8148 T8149 arg1Of modification,in
R6544 T8149 T8151 arg1Of in,to
R6545 T8150 T8149 arg2Of addition,in
R699 T673 T671 arg2Of defect,explaining
R700 T673 T672 arg1Of defect,the
R701 T673 T674 arg1Of defect,in
R8010 T10199 T10198 arg1Of results,Our
R8011 T10199 T10200 arg1Of results,indicate
R8012 T10204 T10202 arg1Of HOIP,the
R8013 T10204 T10203 arg1Of HOIP,protein
R8014 T10204 T10205 arg1Of HOIP,is
R8015 T10204 T10206 arg1Of HOIP,critical
R8016 T10205 T10200 arg2Of is,indicate
R8017 T10205 T10201 arg1Of is,that
R8018 T10206 T10205 arg2Of critical,is
R8019 T10206 T10207 arg1Of critical,for
R8020 T10209 T10207 arg2Of signals,for
R8021 T10209 T10208 arg1Of signals,CD40-induced
R8022 T10209 T10210 arg1Of signals,that
R8023 T10209 T10211 arg1Of signals,regulate
R8024 T10214 T10211 arg2Of function,regulate
R8025 T10214 T10212 arg1Of function,B
R8026 T10214 T10213 arg1Of function,cell
R8027 T10216 T10215 arg1Of data,Our
R8028 T10216 T10217 arg1Of data,show
R8029 T10221 T10219 arg1Of responses,HOIP-dependent
R8030 T10221 T10220 arg1Of responses,cellular
R8031 T10221 T10222 arg1Of responses,include
R8032 T10222 T10217 arg2Of include,show
R8033 T10222 T10218 arg1Of include,that
R8034 T10224 T10222 arg2Of upregulation,include
R8035 T10224 T10223 arg1Of upregulation,CD40-mediated
R8036 T10224 T10225 arg1Of upregulation,of
R8037 T10227 T10228 arg1Of expression,and
R8038 T10228 T10225 arg2Of and,of
R8039 T10228 T10226 arg1Of and,CD80
R8040 T10228 T10230 arg1Of and,of
R8041 T10229 T10228 arg2Of synthesis,and
R8042 T10233 T10230 arg2Of transcripts,of
R8043 T10233 T10231 arg1Of transcripts,germline
R8044 T10233 T10232 arg1Of transcripts,RNA
R8045 T10233 T10234 arg1Of transcripts,from
R8046 T10239 T10234 arg2Of locus,from
R8047 T10239 T10235 arg1Of locus,the
R8048 T10239 T10236 arg1Of locus,immunoglobulin
R8049 T10239 T10237 arg1Of locus,heavy
R8050 T10239 T10238 arg1Of locus,chain
R8051 T10239 T10240 arg1Of locus,","
R8052 T10242 T10240 arg2Of events,","
R8053 T10242 T10241 arg1Of events,two
R8054 T10242 T10243 arg1Of events,that
R8055 T10242 T10244 arg1Of events,are
R8056 T10242 T10245 arg1Of events,important
R8057 T10245 T10244 arg2Of important,are
R8058 T10245 T10246 arg1Of important,for
R8059 T10250 T10246 arg2Of responses,for
R8060 T10250 T10247 arg1Of responses,T-cell-dependent
R8061 T10250 T10248 arg1Of responses,antibody-mediated
R8062 T10250 T10249 arg1Of responses,immune
R8063 T10254 T10251 arg2Of level,At
R8064 T10254 T10252 arg1Of level,the
R8065 T10254 T10253 arg1Of level,molecular
R8066 T10257 T10256 arg1Of data,our
R8067 T10257 T10258 arg1Of data,indicate
R8068 T10258 T10251 arg1Of indicate,At
R8069 T10258 T10255 arg1Of indicate,","
R8070 T10258 T10285 arg1Of indicate,to
R8071 T10261 T10260 arg1Of functions,HOIP
R8072 T10261 T10262 arg1Of functions,downstream
R8073 T10261 T10273 arg1Of functions,has
R8074 T10262 T10263 arg1Of downstream,of
R8075 T10264 T10263 arg2Of TRAF2,of
R8076 T10264 T10265 arg1Of TRAF2,in
R8077 T10269 T10267 arg1Of pathway,CD40
R8078 T10269 T10268 arg1Of pathway,signaling
R8079 T10269 T10270 arg1Of pathway,and
R8080 T10270 T10265 arg2Of and,in
R8081 T10270 T10266 arg1Of and,the
R8082 T10272 T10270 arg2Of HOIP,and
R8083 T10272 T10271 arg1Of HOIP,that
R8084 T10273 T10258 arg2Of has,indicate
R8085 T10273 T10259 arg1Of has,that
R8086 T10273 T10277 arg1Of has,in
R8087 T10276 T10273 arg2Of role,has
R8088 T10276 T10274 arg1Of role,a
R8089 T10276 T10275 arg1Of role,key
R8090 T10278 T10277 arg2Of promoting,in
R8091 T10280 T10278 arg2Of recruitment,promoting
R8092 T10280 T10279 arg1Of recruitment,the
R8093 T10280 T10281 arg1Of recruitment,of
R8094 T10284 T10281 arg2Of complex,of
R8095 T10284 T10282 arg1Of complex,the
R8096 T10284 T10283 arg1Of complex,IKK
R8097 T10286 T10285 arg2Of CD40,to
R8098 T10287 T10288 arg1Of Consistent,with
R8099 T10289 T10288 arg2Of this,with
R8100 T10292 T10291 arg1Of activation,CD40-induced
R8101 T10292 T10293 arg1Of activation,of
R8102 T10292 T10295 arg1Of activation,is
R8103 T10292 T10296 arg1Of activation,dependent
R8104 T10294 T10293 arg2Of NF-κB,of
R8105 T10295 T10287 arg1Of is,Consistent
R8106 T10295 T10290 arg1Of is,","
R8107 T10296 T10295 arg2Of dependent,is
R8108 T10296 T10297 arg1Of dependent,on
R8109 T10299 T10297 arg2Of presence,on
R8110 T10299 T10298 arg1Of presence,the
R8111 T10299 T10300 arg1Of presence,of
R8112 T10301 T10300 arg2Of HOIP,of
R8113 T10303 T10302 arg2Of addition,In
R8114 T10306 T10305 arg1Of data,our
R8115 T10306 T10307 arg1Of data,show
R8116 T10307 T10302 arg1Of show,In
R8117 T10307 T10304 arg1Of show,","
R8118 T10309 T10310 arg1Of HOIP,facilitates
R8119 T10310 T10307 arg2Of facilitates,show
R8120 T10310 T10308 arg1Of facilitates,that
R8121 T10310 T10315 arg1Of facilitates,in
R8122 T10312 T10310 arg2Of activation,facilitates
R8123 T10312 T10311 arg1Of activation,the
R8124 T10312 T10313 arg1Of activation,of
R8125 T10314 T10313 arg2Of JNK,of
R8126 T10316 T10315 arg2Of response,in
R8127 T10316 T10317 arg1Of response,to
R8128 T10319 T10317 arg2Of engagement,to
R8129 T10319 T10318 arg1Of engagement,CD40
R8130 T10323 T10322 arg1Of findings,our
R8131 T10323 T10324 arg1Of findings,provide
R8132 T10324 T10320 arg1Of provide,Together
R8133 T10324 T10321 arg1Of provide,","
R8134 T10325 T10324 arg2Of support,provide
R8135 T10325 T10326 arg1Of support,for
R8136 T10328 T10326 arg2Of conclusion,for
R8137 T10328 T10327 arg1Of conclusion,the
R8138 T10330 T10331 arg1Of HOIP,is
R8139 T10331 T10328 arg2Of is,conclusion
R8140 T10331 T10329 arg1Of is,that
R8141 T10334 T10331 arg2Of component,is
R8142 T10334 T10332 arg1Of component,a
R8143 T10334 T10333 arg1Of component,key
R8144 T10334 T10335 arg1Of component,of
R8145 T10339 T10335 arg2Of pathway,of
R8146 T10339 T10336 arg1Of pathway,the
R8147 T10339 T10337 arg1Of pathway,CD40
R8148 T10339 T10338 arg1Of pathway,signaling
R8149 T10342 T10340 arg2Of importance,Given
R8150 T10342 T10341 arg1Of importance,the
R8151 T10342 T10343 arg1Of importance,of
R8152 T10342 T10346 arg1Of importance,in
R8153 T10345 T10343 arg2Of signaling,of
R8154 T10345 T10344 arg1Of signaling,CD40
R8155 T10348 T10349 arg1Of cellular,and
R8156 T10350 T10349 arg2Of humoral,and
R8157 T10352 T10346 arg2Of responses,in
R8158 T10352 T10347 arg1Of responses,both
R8159 T10352 T10348 arg1Of responses,cellular
R8160 T10352 T10350 arg1Of responses,humoral
R8161 T10352 T10351 arg1Of responses,immune
R8162 T10355 T10354 arg1Of results,our
R8163 T10355 T10356 arg1Of results,indicate
R8164 T10356 T10340 arg1Of indicate,Given
R8165 T10356 T10353 arg1Of indicate,","
R8166 T10358 T10359 arg1Of HOIP,has
R8167 T10359 T10356 arg2Of has,indicate
R8168 T10359 T10357 arg1Of has,that
R8169 T10359 T10363 arg1Of has,in
R8170 T10362 T10359 arg2Of role,has
R8171 T10362 T10360 arg1Of role,a
R8172 T10362 T10361 arg1Of role,critical
R8173 T10365 T10363 arg2Of regulation,in
R8174 T10365 T10364 arg1Of regulation,the
R8175 T10365 T10366 arg1Of regulation,of
R8176 T10369 T10366 arg2Of system,of
R8177 T10369 T10367 arg1Of system,the
R8178 T10369 T10368 arg1Of system,immune
R8179 T10372 T10370 arg1Of properties,The
R8180 T10372 T10371 arg1Of properties,functional
R8181 T10372 T10373 arg1Of properties,of
R8182 T10372 T10375 arg1Of properties,have
R8183 T10372 T10377 arg1Of properties,been
R8184 T10372 T10379 arg2Of properties,characterized
R8185 T10374 T10373 arg2Of HOIP,of
R8186 T10379 T10375 arg2Of characterized,have
R8187 T10379 T10376 arg1Of characterized,only
R8188 T10379 T10377 arg2Of characterized,been
R8189 T10379 T10378 arg1Of characterized,partially
R8190 T10381 T10380 arg1Of studies,Initial
R8191 T10381 T10382 arg1Of studies,showed
R8192 T10384 T10385 arg1Of HOIP,and
R8193 T10385 T10390 arg1Of and,are
R8194 T10389 T10385 arg2Of HOIL-1,and
R8195 T10389 T10386 arg1Of HOIL-1,the
R8196 T10389 T10387 arg2Of HOIL-1,related
R8197 T10389 T10388 arg1Of HOIL-1,protein
R8198 T10390 T10382 arg2Of are,showed
R8199 T10390 T10383 arg1Of are,that
R8200 T10391 T10390 arg2Of components,are
R8201 T10391 T10392 arg1Of components,of
R8202 T10394 T10395 arg1Of large,(
R8203 T10397 T10395 arg2Of kDa,(
R8204 T10397 T10396 arg1Of kDa,∼600
R8205 T10398 T10395 arg3Of ),(
R8206 T10400 T10392 arg2Of complex,of
R8207 T10400 T10393 arg1Of complex,a
R8208 T10400 T10394 arg1Of complex,large
R8209 T10400 T10399 arg1Of complex,protein
R8210 T10400 T10401 arg1Of complex,capable
R8211 T10401 T10402 arg1Of capable,of
R8212 T10403 T10402 arg2Of synthesizing,of
R8213 T10406 T10403 arg2Of chains,synthesizing
R8214 T10406 T10404 arg1Of chains,linear
R8215 T10406 T10405 arg1Of chains,polyubiquitin
R8216 T10406 T10407 arg1Of chains,[
R8217 T10408 T10407 arg2Of 5,[
R8218 T10409 T10407 arg3Of ],[
R8219 T10411 T10410 arg1Of studies,Subsequent
R8220 T10411 T10412 arg1Of studies,showed
R8221 T10411 T10422 arg1Of studies,facilitate
R8222 T10412 T10421 arg1Of showed,and
R8223 T10416 T10414 arg1Of complex,a
R8224 T10416 T10415 arg1Of complex,HOIP-containing
R8225 T10416 T10417 arg1Of complex,can
R8226 T10416 T10418 arg1Of complex,interact
R8227 T10418 T10412 arg2Of interact,showed
R8228 T10418 T10413 arg1Of interact,that
R8229 T10418 T10417 arg2Of interact,can
R8230 T10418 T10419 arg1Of interact,with
R8231 T10420 T10419 arg2Of IKKγ,with
R8232 T10422 T10421 arg2Of facilitate,and
R8233 T10422 T10426 arg1Of facilitate,via
R8234 T10423 T10422 arg2Of activation,facilitate
R8235 T10423 T10424 arg1Of activation,of
R8236 T10425 T10424 arg2Of NF-κB,of
R8237 T10429 T10426 arg2Of pathway,via
R8238 T10429 T10427 arg1Of pathway,the
R8239 T10429 T10428 arg1Of pathway,canonical
R8240 T10429 T10430 arg1Of pathway,[
R8241 T10431 T10430 arg2Of 16,[
R8242 T10432 T10430 arg3Of ],[
R8243 T10434 T10433 arg1Of data,These
R8244 T10434 T10435 arg1Of data,","
R8245 T10434 T10436 arg2Of data,considered
R8246 T10434 T10441 arg1Of data,suggest
R8247 T10436 T10437 arg1Of considered,together
R8248 T10436 T10438 arg1Of considered,with
R8249 T10439 T10438 arg2Of ours,with
R8250 T10441 T10440 arg1Of suggest,","
R8251 T10445 T10443 arg1Of complex,a
R8252 T10445 T10444 arg1Of complex,HOIP-containing
R8253 T10445 T10446 arg1Of complex,mediates
R8254 T10446 T10441 arg2Of mediates,suggest
R8255 T10446 T10442 arg1Of mediates,that
R8256 T10447 T10446 arg2Of recruitment,mediates
R8257 T10447 T10448 arg1Of recruitment,of
R8258 T10447 T10450 arg1Of recruitment,to
R8259 T10449 T10448 arg2Of IKKγ,of
R8260 T10454 T10450 arg2Of complex,to
R8261 T10454 T10451 arg1Of complex,the
R8262 T10454 T10452 arg1Of complex,CD40
R8263 T10454 T10453 arg1Of complex,signaling
R8264 T10456 T10455 arg2Of addition,In
R8265 T10459 T10458 arg1Of HOIP,CD40-associated
R8266 T10459 T10460 arg1Of HOIP,could
R8267 T10459 T10461 arg1Of HOIP,play
R8268 T10461 T10455 arg1Of play,In
R8269 T10461 T10457 arg1Of play,","
R8270 T10461 T10460 arg2Of play,could
R8271 T10461 T10464 arg1Of play,in
R8272 T10463 T10461 arg2Of role,play
R8273 T10463 T10462 arg1Of role,a
R8274 T10465 T10464 arg2Of activating,in
R8275 T10465 T10467 arg1Of activating,after
R8276 T10465 T10474 arg1Of activating,[
R8277 T10466 T10465 arg2Of IKKγ,activating
R8278 T10469 T10467 arg2Of recruitment,after
R8279 T10469 T10468 arg1Of recruitment,its
R8280 T10469 T10470 arg1Of recruitment,to
R8281 T10473 T10470 arg2Of complex,to
R8282 T10473 T10471 arg1Of complex,the
R8283 T10473 T10472 arg1Of complex,signaling
R8284 T10475 T10474 arg2Of 16,[
R8285 T10476 T10474 arg3Of ],[
R8286 T10481 T10477 arg1Of forms,The
R8287 T10481 T10478 arg1Of forms,higher
R8288 T10481 T10479 arg1Of forms,molecular
R8289 T10481 T10480 arg1Of forms,weight
R8290 T10481 T10482 arg1Of forms,of
R8291 T10481 T10485 arg2Of forms,observed
R8292 T10481 T10489 arg1Of forms,would
R8293 T10481 T10490 arg1Of forms,be
R8294 T10481 T10491 arg1Of forms,consistent
R8295 T10483 T10482 arg2Of IKKγ,of
R8296 T10484 T10485 arg1Of we,observed
R8297 T10485 T10486 arg1Of observed,in
R8298 T10488 T10486 arg2Of immunoprecipitates,in
R8299 T10488 T10487 arg1Of immunoprecipitates,CD40
R8300 T10490 T10489 arg2Of be,would
R8301 T10491 T10490 arg2Of consistent,be
R8302 T10491 T10492 arg1Of consistent,with
R8303 T10494 T10492 arg2Of presence,with
R8304 T10494 T10493 arg1Of presence,the
R8305 T10494 T10495 arg1Of presence,of
R8306 T10497 T10495 arg2Of modifications,of
R8307 T10497 T10496 arg1Of modifications,post-transcriptional
R8308 T10497 T10498 arg1Of modifications,including
R8309 T10499 T10500 arg1Of phosphorylation,and
R8310 T10500 T10498 arg2Of and,including
R8311 T10500 T10502 arg1Of and,","
R8312 T10500 T10503 arg1Of and,which
R8313 T10500 T10504 arg1Of and,have
R8314 T10500 T10505 arg1Of and,been
R8315 T10500 T10506 arg2Of and,suggested
R8316 T10500 T10508 arg1Of and,reduce
R8317 T10500 T10510 arg1Of and,enhance
R8318 T10501 T10500 arg2Of ubiquitination,and
R8319 T10506 T10504 arg2Of suggested,have
R8320 T10506 T10505 arg2Of suggested,been
R8321 T10508 T10509 arg1Of reduce,or
R8322 T10509 T10506 arg3Of or,suggested
R8323 T10509 T10507 arg1Of or,to
R8324 T10509 T10518 arg1Of or,","
R8325 T10509 T10519 arg1Of or,[
R8326 T10510 T10509 arg2Of enhance,or
R8327 T10512 T10508 arg2Of activity,reduce
R8328 T10512 T10510 arg2Of activity,enhance
R8329 T10512 T10511 arg1Of activity,IKKγ
R8330 T10512 T10513 arg1Of activity,","
R8331 T10512 T10514 arg1Of activity,respectively
R8332 T10512 T10515 arg1Of activity,[
R8333 T10516 T10515 arg2Of 16,[
R8334 T10517 T10515 arg3Of ],[
R8335 T10520 T10519 arg2Of 17,[
R8336 T10521 T10519 arg3Of ],[
R8337 T10523 T10522 arg1Of mechanisms,The
R8338 T10523 T10524 arg2Of mechanisms,by
R8339 T10523 T10525 arg1Of mechanisms,which
R8340 T10523 T10536 arg2Of mechanisms,by
R8341 T10523 T10537 arg1Of mechanisms,which
R8342 T10523 T10543 arg1Of mechanisms,remain
R8343 T10523 T10544 arg1Of mechanisms,unclear
R8344 T10526 T10527 arg1Of HOIP,mediates
R8345 T10527 T10524 arg1Of mediates,by
R8346 T10527 T10535 arg1Of mediates,and
R8347 T10528 T10527 arg2Of recruitment,mediates
R8348 T10528 T10529 arg1Of recruitment,of
R8349 T10528 T10533 arg1Of recruitment,to
R8350 T10532 T10529 arg2Of complex,of
R8351 T10532 T10530 arg1Of complex,the
R8352 T10532 T10531 arg1Of complex,IKK
R8353 T10534 T10533 arg2Of CD40,to
R8354 T10538 T10539 arg1Of HOIP,is
R8355 T10538 T10540 arg2Of HOIP,recruited
R8356 T10540 T10535 arg2Of recruited,and
R8357 T10540 T10536 arg1Of recruited,by
R8358 T10540 T10539 arg2Of recruited,is
R8359 T10540 T10541 arg1Of recruited,to
R8360 T10542 T10541 arg2Of CD40,to
R8361 T10544 T10543 arg2Of unclear,remain
R8362 T10547 T10545 arg1Of study,A
R8363 T10547 T10546 arg1Of study,previous
R8364 T10547 T10548 arg1Of study,indicates
R8365 T10550 T10551 arg1Of HOIP,may
R8366 T10550 T10552 arg1Of HOIP,mediate
R8367 T10552 T10548 arg2Of mediate,indicates
R8368 T10552 T10549 arg1Of mediate,that
R8369 T10552 T10551 arg2Of mediate,may
R8370 T10552 T10562 arg1Of mediate,","
R8371 T10552 T10563 modOf mediate,suggesting
R8372 T10554 T10552 arg2Of contacts,mediate
R8373 T10554 T10553 arg1Of contacts,direct
R8374 T10554 T10555 arg1Of contacts,with
R8375 T10558 T10555 arg2Of complex,with
R8376 T10558 T10556 arg1Of complex,the
R8377 T10558 T10557 arg1Of complex,IKK
R8378 T10558 T10559 arg1Of complex,[
R8379 T10560 T10559 arg2Of 16,[
R8380 T10561 T10559 arg3Of ],[
R8381 T10565 T10566 arg1Of it,functions
R8382 T10566 T10563 arg2Of functions,suggesting
R8383 T10566 T10564 arg1Of functions,that
R8384 T10566 T10567 arg1Of functions,as
R8385 T10569 T10567 arg2Of adaptor,as
R8386 T10569 T10568 arg1Of adaptor,an
R8387 T10569 T10570 arg1Of adaptor,for
R8388 T10572 T10570 arg2Of recruitment,for
R8389 T10572 T10571 arg1Of recruitment,the
R8390 T10572 T10573 arg1Of recruitment,of
R8391 T10572 T10577 arg1Of recruitment,to
R8392 T10576 T10573 arg2Of complex,of
R8393 T10576 T10574 arg1Of complex,the
R8394 T10576 T10575 arg1Of complex,IKK
R8395 T10578 T10577 arg2Of CD40,to
R8396 T10584 T10581 arg1Of activity,the
R8397 T10584 T10582 arg1Of activity,ubiquitin
R8398 T10584 T10583 arg1Of activity,ligase
R8399 T10584 T10585 arg1Of activity,of
R8400 T10584 T10587 arg1Of activity,suggests
R8401 T10586 T10585 arg2Of HOIP,of
R8402 T10587 T10579 arg1Of suggests,However
R8403 T10587 T10580 arg1Of suggests,","
R8404 T10589 T10590 arg1Of it,is
R8405 T10589 T10591 arg1Of it,more
R8406 T10590 T10587 arg2Of is,suggests
R8407 T10590 T10588 arg1Of is,that
R8408 T10591 T10590 arg2Of more,is
R8409 T10591 T10592 arg1Of more,than
R8410 T10596 T10592 arg2Of molecule,than
R8411 T10596 T10593 arg1Of molecule,a
R8412 T10596 T10594 arg1Of molecule,simple
R8413 T10596 T10595 arg1Of molecule,adapter
R8414 T10598 T10599 arg1Of IKKγ,appears
R8415 T10598 T10600 arg1Of IKKγ,capable
R8416 T10598 T10602 arg1Of IKKγ,binding
R8417 T10599 T10597 arg2Of appears,As
R8418 T10599 T10608 arg1Of appears,","
R8419 T10599 T10609 arg1Of appears,[
R8420 T10600 T10599 arg2Of capable,appears
R8421 T10600 T10601 arg1Of capable,of
R8422 T10602 T10601 arg2Of binding,of
R8423 T10604 T10602 arg2Of polyubiquitin,binding
R8424 T10604 T10603 arg1Of polyubiquitin,linear
R8425 T10604 T10605 arg1Of polyubiquitin,[
R8426 T10606 T10605 arg2Of 18,[
R8427 T10607 T10605 arg3Of ],[
R8428 T10610 T10609 arg2Of 19,[
R8429 T10611 T10609 arg3Of ],[
R8430 T10614 T10597 arg1Of is,As
R8431 T10614 T10612 arg1Of is,","
R8432 T10615 T10614 arg2Of possible,is
R8433 T10617 T10618 arg1Of HOIP,directs
R8434 T10618 T10624 arg1Of directs,on
R8435 T10618 T10629 arg1Of directs,and
R8436 T10619 T10618 arg2Of formation,directs
R8437 T10619 T10620 arg1Of formation,of
R8438 T10623 T10620 arg2Of chains,of
R8439 T10623 T10621 arg1Of chains,linear
R8440 T10623 T10622 arg1Of chains,polyubiquitin
R8441 T10627 T10624 arg2Of factor,on
R8442 T10627 T10625 arg1Of factor,a
R8443 T10627 T10626 arg1Of factor,CD40-associated
R8444 T10629 T10613 arg1Of and,it
R8445 T10629 T10614 arg1Of and,is
R8446 T10629 T10615 arg1Of and,possible
R8447 T10629 T10616 arg1Of and,that
R8448 T10629 T10628 arg1Of and,","
R8449 T10630 T10631 arg1Of it,is
R8450 T10631 T10629 arg2Of is,and
R8451 T10633 T10631 arg2Of chains,is
R8452 T10633 T10632 arg1Of chains,these
R8453 T10633 T10634 arg1Of chains,that
R8454 T10633 T10635 arg1Of chains,serve
R8455 T10633 T10637 arg1Of chains,recruit
R8456 T10637 T10635 arg2Of recruit,serve
R8457 T10637 T10636 arg1Of recruit,to
R8458 T10638 T10637 arg2Of IKKγ,recruit
R8459 T10638 T10639 arg1Of IKKγ,to
R8460 T10643 T10639 arg2Of complex,to
R8461 T10643 T10640 arg1Of complex,the
R8462 T10643 T10641 arg1Of complex,CD40
R8463 T10643 T10642 arg1Of complex,signaling
R8464 T10646 T10644 arg1Of interactions,The
R8465 T10646 T10645 arg1Of interactions,molecular
R8466 T10646 T10647 arg1Of interactions,necessary
R8467 T10646 T10659 arg1Of interactions,remain
R8468 T10646 T10661 arg1Of interactions,be
R8469 T10646 T10663 arg2Of interactions,characterized
R8470 T10647 T10648 arg1Of necessary,for
R8471 T10647 T10653 arg1Of necessary,to
R8472 T10649 T10648 arg2Of recruitment,for
R8473 T10649 T10650 arg1Of recruitment,of
R8474 T10652 T10650 arg2Of itself,of
R8475 T10652 T10651 arg1Of itself,HOIP
R8476 T10657 T10653 arg2Of complex,to
R8477 T10657 T10654 arg1Of complex,the
R8478 T10657 T10655 arg1Of complex,CD40
R8479 T10657 T10656 arg1Of complex,signaling
R8480 T10659 T10658 arg1Of remain,also
R8481 T10663 T10659 arg2Of characterized,remain
R8482 T10663 T10660 arg1Of characterized,to
R8483 T10663 T10661 arg2Of characterized,be
R8484 T10663 T10662 arg1Of characterized,fully
R8485 T10664 T10666 arg1Of We,showed
R8486 T10666 T10665 arg1Of showed,previously
R8487 T10669 T10668 arg1Of recruitment,the
R8488 T10669 T10670 arg1Of recruitment,of
R8489 T10669 T10672 arg1Of recruitment,to
R8490 T10669 T10676 arg1Of recruitment,is
R8491 T10669 T10677 arg1Of recruitment,TRAF2-dependent
R8492 T10671 T10670 arg2Of HOIP,of
R8493 T10675 T10672 arg2Of complex,to
R8494 T10675 T10673 arg1Of complex,the
R8495 T10675 T10674 arg1Of complex,signaling
R8496 T10676 T10666 arg2Of is,showed
R8497 T10676 T10667 arg1Of is,that
R8498 T10676 T10678 arg1Of is,[
R8499 T10677 T10676 arg2Of TRAF2-dependent,is
R8500 T10679 T10678 arg2Of 8,[
R8501 T10680 T10678 arg3Of ],[
R8502 T10683 T10684 arg1Of TRAF2,and
R8503 T10684 T10687 arg1Of and,interact
R8504 T10685 T10684 arg2Of HOIP,and
R8505 T10687 T10686 arg1Of interact,directly
R8506 T10687 T10689 arg1Of interact,but
R8507 T10689 T10681 arg1Of but,Potentially
R8508 T10689 T10682 arg1Of but,","
R8509 T10689 T10688 arg1Of but,","
R8510 T10691 T10689 arg2Of is,but
R8511 T10692 T10691 arg2Of possible,is
R8512 T10697 T10694 arg1Of activity,the
R8513 T10697 T10695 arg1Of activity,ubiquitin
R8514 T10697 T10696 arg1Of activity,ligase
R8515 T10697 T10698 arg1Of activity,of
R8516 T10697 T10716 arg1Of activity,generates
R8517 T10699 T10698 arg2Of TRAF2,of
R8518 T10699 T10700 arg1Of TRAF2,[
R8519 T10699 T10703 arg1Of TRAF2,(
R8520 T10701 T10700 arg2Of 20,[
R8521 T10702 T10700 arg3Of ],[
R8522 T10706 T10703 arg2Of proteins,(
R8523 T10706 T10704 arg1Of proteins,or
R8524 T10706 T10705 arg1Of proteins,TRAF2-associated
R8525 T10706 T10707 arg1Of proteins,","
R8526 T10706 T10709 arg1Of proteins,as
R8527 T10706 T10712 arg1Of proteins,[
R8528 T10709 T10708 arg1Of as,such
R8529 T10711 T10709 arg2Of cIAPs,as
R8530 T10711 T10710 arg1Of cIAPs,the
R8531 T10713 T10712 arg2Of 21,[
R8532 T10714 T10712 arg3Of ],[
R8533 T10715 T10703 arg3Of ),(
R8534 T10716 T10690 arg1Of generates,it
R8535 T10716 T10691 arg1Of generates,is
R8536 T10716 T10692 arg1Of generates,possible
R8537 T10716 T10693 arg1Of generates,that
R8538 T10719 T10716 arg2Of chains,generates
R8539 T10719 T10717 arg1Of chains,K63-linked
R8540 T10719 T10718 arg1Of chains,polyubiquitin
R8541 T10719 T10720 arg2Of chains,to
R8542 T10719 T10721 arg1Of chains,which
R8543 T10722 T10723 arg1Of HOIP,can
R8544 T10751 T10749 arg2Of signaling,in
R8545 T10751 T10750 arg1Of signaling,CD40
R8546 T10722 T10724 arg1Of HOIP,bind
R8547 T10722 T10727 arg1Of HOIP,associate
R8548 T10724 T10723 arg2Of bind,can
R8549 T10754 T10755 arg1Of signals,and
R8550 T10755 T10753 arg1Of and,the
R8551 T10755 T10757 arg2Of and,tested
R8552 T10755 T10759 arg1Of and,are
R8553 T10755 T10760 arg1Of and,dependent
R8554 T10756 T10755 arg2Of functions,and
R8555 T10757 T10758 arg1Of tested,here
R8556 T10724 T10725 arg1Of bind,and
R8557 T10725 T10720 arg1Of and,to
R8558 T10727 T10725 arg2Of associate,and
R8559 T10759 T10736 arg1Of are,While
R8560 T10759 T10752 arg1Of are,","
R8561 T10760 T10759 arg2Of dependent,are
R8562 T10760 T10761 arg1Of dependent,upon
R8563 T10727 T10726 arg1Of associate,thus
R8564 T10762 T10765 arg1Of TRAF6,as
R8565 T10727 T10728 arg1Of associate,with
R8566 T10732 T10728 arg2Of complex,with
R8567 T10765 T10761 arg2Of as,upon
R8568 T10765 T10763 arg1Of as,as
R8569 T10765 T10764 arg1Of as,well
R8570 T10766 T10765 arg2Of TRAF2,as
R8571 T10769 T10767 arg2Of experiments,In
R8572 T10769 T10768 arg1Of experiments,previous
R8573 T10769 T10770 arg1Of experiments,with
R8574 T10773 T10770 arg2Of cells,with
R8575 T10773 T10771 arg1Of cells,TRAF-deficient
R8576 T10773 T10772 arg1Of cells,A20.2J
R8577 T10775 T10776 arg1Of we,found
R8578 T10776 T10767 arg1Of found,In
R8579 T10776 T10774 arg1Of found,","
R8580 T10732 T10729 arg1Of complex,the
R8581 T10732 T10730 arg1Of complex,CD40
R8582 T10732 T10731 arg1Of complex,signaling
R8583 T10779 T10778 arg1Of activation,the
R8584 T10732 T10733 arg1Of complex,[
R8585 T10779 T10780 arg1Of activation,of
R8586 T10779 T10782 arg1Of activation,by
R8587 T10779 T10784 arg1Of activation,could
R8588 T10779 T10785 arg1Of activation,be
R8589 T10779 T10786 arg2Of activation,mediated
R8590 T10781 T10780 arg2Of NF-κB,of
R8591 T10783 T10782 arg2Of CD40,by
R8592 T10734 T10733 arg2Of 22,[
R8593 T10786 T10776 arg2Of mediated,found
R8594 T10786 T10777 arg1Of mediated,that
R8595 T10786 T10784 arg2Of mediated,could
R8596 T10786 T10785 arg2Of mediated,be
R8597 T10786 T10792 arg1Of mediated,","
R8598 T10786 T10793 arg1Of mediated,while
R8599 T10789 T10790 arg1Of TRAF2,or
R8600 T10735 T10733 arg3Of ],[
R8601 T10790 T10786 arg1Of or,mediated
R8602 T10790 T10787 arg2Of or,by
R8603 T10790 T10788 arg1Of or,either
R8604 T10791 T10790 arg2Of TRAF6,or
R8605 T10739 T10737 arg1Of work,our
R8606 T10794 T10795 arg1Of activation,of
R8607 T10794 T10797 arg1Of activation,by
R8608 T10794 T10799 arg1Of activation,was
R8609 T10794 T10801 arg1Of activation,dependent
R8610 T10796 T10795 arg2Of JNK,of
R8611 T10798 T10797 arg2Of CD40,by
R8612 T10799 T10793 arg2Of was,while
R8613 T10799 T10805 arg1Of was,[
R8614 T10739 T10738 arg1Of work,previous
R8615 T10801 T10799 arg2Of dependent,was
R8616 T10801 T10800 arg1Of dependent,largely
R8617 T10801 T10802 arg1Of dependent,on
R8618 T10803 T10802 arg2Of TRAF6,on
R8619 T10803 T10804 arg1Of TRAF6,alone
R8620 T10739 T10740 arg1Of work,indicated
R8621 T10806 T10805 arg2Of 8,[
R8622 T10740 T10736 arg2Of indicated,While
R8623 T10807 T10805 arg3Of ],[
R8624 T10809 T10808 arg1Of deficiency,HOIP
R8625 T10809 T10810 arg1Of deficiency,compromises
R8626 T10743 T10742 arg1Of important,potentially
R8627 T10810 T10819 arg1Of compromises,","
R8628 T10744 T10740 arg2Of link,indicated
R8629 T10813 T10810 arg2Of activation,compromises
R8630 T10813 T10811 arg1Of activation,the
R8631 T10813 T10812 arg1Of activation,CD40-mediated
R8632 T10813 T10814 arg1Of activation,of
R8633 T10744 T10741 arg1Of link,a
R8634 T10816 T10817 arg1Of NF-κB,and
R8635 T10744 T10743 arg1Of link,important
R8636 T10817 T10814 arg2Of and,of
R8637 T10817 T10815 arg1Of and,both
R8638 T10744 T10745 arg1Of link,between
R8639 T10818 T10817 arg2Of JNK,and
R8640 T10746 T10747 arg1Of TRAF2,and
R8641 T10820 T10810 arg3Of indicating,compromises
R8642 T10747 T10745 arg2Of and,between
R8643 T10822 T10823 arg2Of signals,mediated
R8644 T10822 T10830 arg1Of signals,pass
R8645 T10747 T10749 arg1Of and,in
R8646 T10748 T10747 arg2Of HOIP,and
R8647 T10826 T10827 arg1Of TRAF2,and
R8648 T10827 T10823 arg1Of and,mediated
R8649 T10827 T10824 arg2Of and,by
R8650 T10827 T10825 arg1Of and,both
R8651 T10828 T10827 arg2Of TRAF6,and
R8652 T10848 T10847 arg2Of TRAF6-dependent,while
R8653 T10830 T10820 arg2Of pass,indicating
R8654 T10830 T10821 arg1Of pass,that
R8655 T10830 T10829 arg1Of pass,likely
R8656 T10830 T10831 arg1Of pass,through
R8657 T10832 T10831 arg2Of HOIP,through
R8658 T10852 T10837 arg2Of compromised,demonstrated
R8659 T10835 T10833 arg1Of work,Our
R8660 T10835 T10834 arg1Of work,previous
R8661 T10835 T10837 arg1Of work,demonstrated
R8662 T10837 T10836 arg1Of demonstrated,also
R8663 T10841 T10839 arg1Of activation,the
R8664 T10841 T10840 arg1Of activation,CD40-mediated
R8665 T10841 T10842 arg1Of activation,of
R8666 T10841 T10850 arg1Of activation,was
R8667 T10841 T10852 arg2Of activation,compromised
R8668 T10843 T10844 arg1Of NF-κB,and
R8669 T10844 T10842 arg2Of and,of
R8670 T10845 T10844 arg2Of JNK,and
R8671 T10852 T10838 arg1Of compromised,that
R8672 T10852 T10846 arg1Of compromised,","
R8673 T10852 T10847 arg1Of compromised,while
R8674 T10852 T10849 arg1Of compromised,","
R8675 T10852 T10850 arg2Of compromised,was
R8676 T10852 T10851 arg1Of compromised,not
R8677 T10852 T10880 arg1Of compromised,in
R8678 T10852 T10882 arg1Of compromised,[
R8679 T10855 T10854 arg1Of disruption,the
R8680 T10855 T10856 arg1Of disruption,of
R8681 T10855 T10862 arg1Of disruption,in
R8682 T10855 T10868 arg1Of disruption,or
R8683 T10859 T10856 arg2Of site,of
R8684 T10944 T10942 arg2Of results,","
R8685 T10944 T10943 arg1Of results,our
R8686 T10945 T10934 arg1Of and,Although
R8687 T10946 T10947 arg1Of those,of
R8688 T10946 T10953 arg1Of those,","
R8689 T10946 T10954 arg1Of those,[
R8690 T10946 T10957 arg1Of those,support
R8691 T10949 T10947 arg2Of groups,of
R8692 T10949 T10948 arg1Of groups,other
R8693 T10949 T10950 arg1Of groups,[
R8694 T10951 T10950 arg2Of 16,[
R8695 T10859 T10857 arg1Of site,the
R8696 T10952 T10950 arg3Of ],[
R8697 T10859 T10858 arg1Of site,binding
R8698 T10859 T10860 arg1Of site,for
R8699 T10955 T10954 arg2Of 22,[
R8700 T10861 T10860 arg2Of TRAF6,for
R8701 T10956 T10954 arg3Of ],[
R8702 T10957 T10945 arg2Of support,and
R8703 T10959 T10957 arg2Of possibility,support
R8704 T10959 T10958 arg1Of possibility,the
R8705 T10865 T10862 arg2Of domain,in
R8706 T10961 T10962 arg1Of HOIP,is
R8707 T10961 T10963 arg1Of HOIP,important
R8708 T10962 T10959 arg2Of is,possibility
R8709 T10962 T10960 arg1Of is,that
R8710 T10963 T10962 arg2Of important,is
R8711 T10963 T10964 arg1Of important,in
R8712 T10865 T10863 arg1Of domain,the
R8713 T10865 T10864 arg1Of domain,cytoplasmic
R8714 T10865 T10866 arg1Of domain,of
R8715 T10967 T10964 arg2Of pathways,in
R8716 T10967 T10965 arg1Of pathways,many
R8717 T10967 T10966 arg1Of pathways,signaling
R8718 T10967 T10968 arg2Of pathways,in
R8719 T10967 T10969 arg1Of pathways,which
R8720 T10967 T10975 arg1Of pathways,","
R8721 T10967 T10976 arg1Of pathways,including
R8722 T10970 T10971 arg1Of TRAF2,or
R8723 T10867 T10866 arg2Of CD40,of
R8724 T10971 T10973 arg1Of or,are
R8725 T10971 T10974 arg2Of or,involved
R8726 T10972 T10971 arg2Of TRAF6,or
R8727 T10974 T10968 arg1Of involved,in
R8728 T10974 T10973 arg2Of involved,are
R8729 T10868 T10852 arg1Of or,compromised
R8730 T10977 T10976 arg2Of those,including
R8731 T10977 T10978 arg2Of those,associated
R8732 T10868 T10853 arg2Of or,by
R8733 T10978 T10979 arg1Of associated,with
R8734 T10869 T10868 arg2Of deletion,or
R8735 T10981 T10979 arg2Of members,with
R8736 T10981 T10980 arg1Of members,various
R8737 T10869 T10870 arg1Of deletion,of
R8738 T10981 T10982 arg1Of members,of
R8739 T10874 T10870 arg2Of domain,of
R8740 T10986 T10983 arg1Of superfamily,the
R8741 T10986 T10984 arg1Of superfamily,TNF
R8742 T10986 T10985 arg1Of superfamily,receptor
R8743 T10986 T10987 arg1Of superfamily,and
R8744 T10987 T10982 arg2Of and,of
R8745 T10990 T10987 arg2Of receptors,and
R8746 T10990 T10988 arg1Of receptors,the
R8747 T10990 T10989 arg1Of receptors,Toll-like
R8748 T10874 T10871 arg1Of domain,the
R8749 T10874 T10872 arg1Of domain,receptor
R8750 T10993 T10991 arg1Of importance,The
R8751 T10993 T10992 arg1Of importance,potential
R8752 T10993 T10994 arg1Of importance,of
R8753 T10993 T10996 arg1Of importance,in
R8754 T10993 T11003 arg1Of importance,is
R8755 T10993 T11005 arg2Of importance,supported
R8756 T10995 T10994 arg2Of HOIP,of
R8757 T10874 T10873 arg1Of domain,binding
R8758 T10874 T10875 arg1Of domain,(
R8759 T10878 T10875 arg2Of domain,(
R8760 T10998 T10997 arg1Of function,immune
R8761 T10998 T10999 arg1Of function,and
R8762 T10999 T10996 arg2Of and,in
R8763 T11002 T10999 arg2Of signaling,and
R8764 T11002 T11000 arg1Of signaling,TNFR
R8765 T11002 T11001 arg1Of signaling,family
R8766 T10878 T10876 arg1Of domain,the
R8767 T11005 T11003 arg2Of supported,is
R8768 T11005 T11004 arg1Of supported,further
R8769 T11005 T11006 arg1Of supported,by
R8770 T10878 T10877 arg1Of domain,TRAF-C
R8771 T11009 T11006 arg2Of discovery,by
R8772 T11009 T11007 arg1Of discovery,the
R8773 T11009 T11008 arg1Of discovery,recent
R8774 T10879 T10875 arg3Of ),(
R8775 T11011 T11012 arg1Of HOIP,interacts
R8776 T11012 T11009 arg2Of interacts,discovery
R8777 T11012 T11010 arg1Of interacts,that
R8778 T11012 T11013 arg1Of interacts,with
R8779 T10881 T10880 arg2Of TRAF6,in
R8780 T11015 T11013 arg2Of protein,with
R8781 T11015 T11014 arg1Of protein,a
R8782 T11015 T11016 arg2Of protein,known
R8783 T11016 T11017 arg1Of known,as
R8784 T10883 T10882 arg2Of 8,[
R8785 T11018 T11017 arg2Of SHARPIN,as
R8786 T11018 T11019 arg1Of SHARPIN,(
R8787 T11018 T11029 arg1Of SHARPIN,","
R8788 T11018 T11030 arg1Of SHARPIN,which
R8789 T11018 T11031 arg1Of SHARPIN,appears
R8790 T11018 T11032 arg1Of SHARPIN,capable
R8791 T11018 T11034 arg1Of SHARPIN,working
R8792 T11024 T11019 arg2Of protein,(
R8793 T11024 T11020 arg1Of protein,SHANK-associated
R8794 T11024 T11021 arg1Of protein,RH
R8795 T11024 T11022 arg1Of protein,domain
R8796 T11024 T11023 arg1Of protein,interacting
R8797 T11024 T11025 arg1Of protein,in
R8798 T10884 T10882 arg3Of ],[
R8799 T11027 T11025 arg2Of density,in
R8800 T11027 T11026 arg1Of density,postsynaptic
R8801 T11028 T11019 arg3Of ),(
R8802 T10886 T10885 arg1Of observations,These
R8803 T11032 T11031 arg2Of capable,appears
R8804 T11032 T11033 arg1Of capable,of
R8805 T11034 T11033 arg2Of working,of
R8806 T11034 T11035 arg1Of working,together
R8807 T11034 T11036 arg1Of working,with
R8808 T10886 T10887 arg1Of observations,indicate
R8809 T10889 T10890 arg1Of TRAF6,need
R8810 T11037 T11038 arg1Of HOIP,and
R8811 T10889 T10893 arg1Of TRAF6,bind
R8812 T11038 T11036 arg2Of and,with
R8813 T11039 T11038 arg2Of HOIL,and
R8814 T11039 T11040 modOf HOIL,to
R8815 T10889 T10898 arg1Of TRAF6,mediate
R8816 T10890 T10887 arg2Of need,indicate
R8817 T10890 T10888 arg1Of need,that
R8818 T10890 T10891 arg1Of need,not
R8819 T11041 T11040 arg1Of mediate,to
R8820 T11041 T11050 arg1Of mediate,","
R8821 T11041 T11051 arg1Of mediate,[
R8822 T11041 T11054 arg1Of mediate,","
R8823 T11041 T11055 arg1Of mediate,[
R8824 T11043 T11041 arg2Of assembly,mediate
R8825 T11043 T11042 arg1Of assembly,the
R8826 T10890 T10892 arg1Of need,directly
R8827 T10893 T10890 arg2Of bind,need
R8828 T11043 T11044 arg1Of assembly,of
R8829 T11046 T11044 arg2Of polyubiquitin,of
R8830 T11046 T11045 arg1Of polyubiquitin,linear
R8831 T11046 T11047 arg1Of polyubiquitin,[
R8832 T10893 T10895 arg1Of bind,in
R8833 T11048 T11047 arg2Of 14,[
R8834 T10893 T10901 arg1Of bind,","
R8835 T10893 T10902 modOf bind,suggesting
R8836 T11049 T11047 arg3Of ],[
R8837 T10894 T10893 arg2Of CD40,bind
R8838 T11052 T11051 arg2Of 23,[
R8839 T11053 T11051 arg3Of ],[
R8840 T10896 T10895 arg2Of order,in
R8841 T11056 T11055 arg2Of 24,[
R8842 T11057 T11055 arg3Of ],[
R8843 T10898 T10895 arg3Of mediate,in
R8844 T11060 T11061 arg1Of mice,with
R8845 T11060 T11065 arg1Of mice,in
R8846 T11060 T11079 arg1Of mice,exhibit
R8847 T10898 T10897 arg1Of mediate,to
R8848 T10900 T10898 arg2Of signals,mediate
R8849 T11064 T11061 arg2Of mutation,with
R8850 T11064 T11062 arg1Of mutation,a
R8851 T11064 T11063 arg1Of mutation,spontaneous
R8852 T11067 T11065 arg2Of gene,in
R8853 T11067 T11066 arg1Of gene,the
R8854 T11067 T11068 arg1Of gene,encoding
R8855 T10900 T10899 arg1Of signals,certain
R8856 T11069 T11068 arg2Of SHARPIN,encoding
R8857 T11069 T11070 arg1Of SHARPIN,(
R8858 T10904 T10902 arg2Of assembly,suggesting
R8859 T11075 T11074 arg2Of cpdm,(
R8860 T11076 T11074 arg3Of ),(
R8861 T10904 T10903 arg1Of assembly,the
R8862 T11077 T11070 arg2Of mice,(
R8863 T11077 T11071 arg1Of mice,chronic
R8864 T11077 T11072 arg1Of mice,proliferative
R8865 T11077 T11073 arg1Of mice,dermatitis
R8866 T11077 T11074 arg1Of mice,(
R8867 T11078 T11070 arg3Of ),(
R8868 T10904 T10905 arg1Of assembly,of
R8869 T11079 T11058 arg1Of exhibit,Interestingly
R8870 T11079 T11059 arg1Of exhibit,","
R8871 T11081 T11080 arg1Of inflammation,chronic
R8872 T11081 T11082 arg1Of inflammation,of
R8873 T11081 T11088 arg1Of inflammation,","
R8874 T10904 T10911 arg2Of assembly,associated
R8875 T11084 T11085 arg1Of skin,and
R8876 T10908 T10905 arg2Of complex,of
R8877 T11085 T11082 arg2Of and,of
R8878 T11085 T11083 arg1Of and,the
R8879 T11087 T11085 arg2Of organs,and
R8880 T11087 T11086 arg1Of organs,internal
R8881 T11088 T11096 arg1Of ",",and
R8882 T11090 T11088 arg2Of development,","
R8883 T11090 T11089 arg1Of development,defective
R8884 T11090 T11091 arg1Of development,of
R8885 T10908 T10906 arg1Of complex,a
R8886 T10908 T10907 arg1Of complex,signaling
R8887 T11094 T11091 arg2Of tissue,of
R8888 T11094 T11092 arg1Of tissue,secondary
R8889 T11094 T11093 arg1Of tissue,lymphoid
R8890 T10911 T10909 arg1Of associated,not
R8891 T11096 T11079 arg2Of and,exhibit
R8892 T11096 T11095 arg1Of and,","
R8893 T11097 T11096 arg2Of defects,and
R8894 T11097 T11098 arg1Of defects,in
R8895 T11100 T11098 arg2Of production,in
R8896 T11100 T11099 arg1Of production,the
R8897 T11100 T11101 arg1Of production,of
R8898 T10911 T10910 arg1Of associated,directly
R8899 T10911 T10912 arg1Of associated,with
R8900 T11104 T11101 arg2Of isotypes,of
R8901 T11104 T11102 arg2Of isotypes,switched
R8902 T11104 T11103 arg1Of isotypes,immunoglobulin
R8903 T11104 T11105 arg1Of isotypes,[
R8904 T11104 T11108 arg1Of isotypes,","
R8905 T11104 T11109 arg1Of isotypes,[
R8906 T10914 T10912 arg2Of receptor,with
R8907 T11106 T11105 arg2Of 17,[
R8908 T11107 T11105 arg3Of ],[
R8909 T11110 T11109 arg2Of 25,[
R8910 T11111 T11109 arg3Of ],[
R8911 T10914 T10913 arg1Of receptor,the
R8912 T10918 T10916 arg1Of complex,such
R8913 T11114 T11113 arg1Of intimate,apparently
R8914 T11116 T11112 arg1Of link,The
R8915 T11116 T11114 arg1Of link,intimate
R8916 T11116 T11115 arg1Of link,functional
R8917 T11116 T11117 arg1Of link,between
R8918 T11116 T11122 arg1Of link,suggests
R8919 T10918 T10917 arg1Of complex,a
R8920 T11118 T11119 arg1Of SHARPIN,and
R8921 T11119 T11117 arg2Of and,between
R8922 T11120 T11119 arg2Of HOIP,and
R8923 T10918 T10919 arg1Of complex,exists
R8924 T10919 T10915 arg2Of exists,If
R8925 T11122 T11121 arg1Of suggests,strongly
R8926 T10922 T10921 arg1Of results,our
R8927 T11125 T11124 arg1Of least,at
R8928 T11126 T11125 arg1Of part,least
R8929 T11126 T11127 arg1Of part,of
R8930 T11126 T11131 arg1Of part,stems
R8931 T11130 T11127 arg2Of phenotype,of
R8932 T10922 T10923 arg1Of results,indicate
R8933 T11130 T11128 arg1Of phenotype,the
R8934 T11130 T11129 arg1Of phenotype,cpdm
R8935 T11131 T11122 arg2Of stems,suggests
R8936 T11131 T11123 arg1Of stems,that
R8937 T11131 T11132 arg1Of stems,from
R8938 T10923 T10915 arg1Of indicate,If
R8939 T11133 T11132 arg2Of defects,from
R8940 T11133 T11134 arg1Of defects,in
R8941 T10923 T10920 arg1Of indicate,","
R8942 T11136 T11137 arg1Of regulation,or
R8943 T10926 T10925 arg1Of absence,the
R8944 T10926 T10927 arg1Of absence,of
R8945 T11137 T11134 arg2Of or,in
R8946 T11137 T11135 arg1Of or,the
R8947 T11137 T11139 arg1Of or,of
R8948 T11138 T11137 arg2Of function,or
R8949 T10926 T10929 arg1Of absence,compromises
R8950 T10928 T10927 arg2Of HOIP,of
R8951 T11140 T11139 arg2Of HOIP,of
R8952 T10929 T10923 arg2Of compromises,indicate
R8953 T10929 T10924 arg1Of compromises,that
R8954 T10929 T10933 arg1Of compromises,well
R8955 T10931 T10929 arg2Of function,compromises
R8956 T10931 T10930 arg1Of function,its
R8957 T10933 T10932 arg1Of well,as
R8958 T10936 T10935 arg1Of experiments,the
R8960 T10936 T10937 arg2Of experiments,presented
R8961 T10936 T10939 arg1Of experiments,focus
R8963 T10937 T10938 arg1Of presented,here
R8965 T10939 T10940 arg1Of focus,on
R8967 T10939 T10945 arg1Of focus,and
R8968 T10941 T10942 arg1Of CD40,","
R8969 T10942 T10940 arg2Of ",",on
R9305 T11984 T11983 arg1Of lines,Cell
R9306 T11990 T11985 arg1Of A20.2J,The
R9307 T11990 T11986 arg1Of A20.2J,mouse
R9308 T11990 T11987 arg1Of A20.2J,B
R9309 T11990 T11988 arg1Of A20.2J,cell
R9310 T11990 T11989 arg1Of A20.2J,line
R9311 T11990 T11991 arg1Of A20.2J,has
R9312 T11990 T11992 arg1Of A20.2J,been
R9313 T11990 T11994 arg2Of A20.2J,described
R9314 T11994 T11991 arg2Of described,has
R9315 T11994 T11992 arg2Of described,been
R9316 T11994 T11993 arg1Of described,previously
R9317 T11994 T11995 arg1Of described,[
R9318 T11994 T11998 arg1Of described,","
R9319 T11994 T11999 arg1Of described,[
R9320 T11996 T11995 arg2Of 26,[
R9321 T11997 T11995 arg3Of ],[
R9322 T12000 T11999 arg2Of 27,[
R9323 T12001 T11999 arg3Of ],[
R9324 T12002 T12003 arg1Of Cells,were
R9325 T12002 T12004 arg2Of Cells,grown
R9326 T12004 T12003 arg2Of grown,were
R9327 T12004 T12005 arg1Of grown,in
R9328 T12006 T12007 arg1Of RPMI,1640
R9329 T12006 T12008 arg1Of RPMI,","
R9330 T12008 T12012 arg1Of ",",","
R9331 T12009 T12010 arg1Of 10,%
R9332 T12011 T12008 arg2Of FCS,","
R9333 T12011 T12009 arg1Of FCS,10
R9334 T12012 T12016 arg1Of ",",","
R9335 T12015 T12012 arg2Of 2-ME,","
R9336 T12015 T12013 arg1Of 2-ME,10
R9337 T12015 T12014 arg1Of 2-ME,µM
R9338 T12016 T12021 arg1Of ",",and
R9339 T12017 T12018 arg1Of 2,mM
R9340 T12019 T12016 arg2Of L-glutamine,","
R9341 T12019 T12017 arg1Of L-glutamine,2
R9342 T12021 T12005 arg2Of and,in
R9343 T12021 T12020 arg1Of and,","
R9344 T12022 T12021 arg2Of antibiotics,and
R9345 T12025 T12023 arg1Of cells,HI-5
R9346 T12025 T12024 arg1Of cells,insect
R9347 T12025 T12026 arg1Of cells,(
R9348 T12025 T12029 arg1Of cells,expressing
R9349 T12025 T12031 arg1Of cells,have
R9350 T12025 T12032 arg1Of cells,been
R9351 T12025 T12033 arg2Of cells,described
R9352 T12027 T12026 arg2Of Invitrogen,(
R9353 T12028 T12026 arg3Of ),(
R9354 T12030 T12029 arg2Of CD154,expressing
R9355 T12033 T12031 arg2Of described,have
R9356 T12033 T12032 arg2Of described,been
R9357 T12033 T12034 arg1Of described,[
R9358 T12035 T12034 arg2Of 28,[
R9359 T12036 T12034 arg3Of ],[
R9468 T12167 T12164 arg1Of antibody,Mouse
R9469 T12167 T12165 arg1Of antibody,anti-birch
R9470 T12167 T12166 arg1Of antibody,profilin
R9471 T12167 T12168 arg1Of antibody,(
R9472 T12167 T12174 arg1Of antibody,","
R9473 T12169 T12168 arg2Of 4A6,(
R9474 T12169 T12170 arg1Of 4A6,[
R9475 T12171 T12170 arg2Of 29,[
R9476 T12172 T12170 arg3Of ],[
R9477 T12173 T12168 arg3Of ),(
R9478 T12174 T12184 arg1Of ",",and
R9479 T12177 T12174 arg2Of CD40,","
R9480 T12177 T12175 arg1Of CD40,rat
R9481 T12177 T12176 arg1Of CD40,anti-mouse
R9482 T12177 T12178 arg1Of CD40,(
R9483 T12179 T12178 arg2Of 1C10,(
R9484 T12179 T12180 arg1Of 1C10,[
R9485 T12181 T12180 arg2Of 30,[
R9486 T12182 T12180 arg3Of ],[
R9487 T12183 T12178 arg3Of ),(
R9488 T12184 T12207 arg1Of and,were
R9489 T12184 T12208 arg2Of and,isolated
R9490 T12189 T12184 arg2Of antibody,and
R9491 T12189 T12185 arg1Of antibody,a
R9492 T12189 T12186 arg1Of antibody,rat
R9493 T12189 T12187 arg1Of antibody,isotype
R9494 T12189 T12188 arg1Of antibody,control
R9495 T12189 T12190 arg1Of antibody,(
R9496 T12191 T12190 arg2Of mAb72,(
R9497 T12191 T12192 arg1Of mAb72,","
R9498 T12191 T12197 arg1Of mAb72,","
R9499 T12191 T12201 arg1Of mAb72,","
R9500 T12196 T12192 arg2Of Bank,","
R9501 T12196 T12193 arg1Of Bank,Developmental
R9502 T12196 T12194 arg1Of Bank,Studies
R9503 T12196 T12195 arg1Of Bank,Hybridoma
R9504 T12198 T12197 arg2Of University,","
R9505 T12198 T12199 arg1Of University,of
R9506 T12200 T12199 arg2Of Iowa,of
R9507 T12203 T12201 arg2Of City,","
R9508 T12203 T12202 arg1Of City,Iowa
R9509 T12203 T12204 arg1Of City,","
R9510 T12205 T12204 arg2Of IA,","
R9511 T12206 T12190 arg3Of ),(
R9512 T12208 T12207 arg2Of isolated,were
R9513 T12208 T12209 arg1Of isolated,from
R9514 T12211 T12209 arg2Of supernatants,from
R9515 T12211 T12210 arg1Of supernatants,hybridoma
R9516 T12214 T12212 arg1Of CD80,FITC-labeled
R9517 T12214 T12213 arg1Of CD80,anti-mouse
R9518 T12214 T12215 arg1Of CD80,and
R9519 T12215 T12220 arg1Of and,were
R9520 T12215 T12221 arg1Of and,from
R9521 T12219 T12215 arg2Of Ab,and
R9522 T12219 T12216 arg1Of Ab,an
R9523 T12219 T12217 arg1Of Ab,isotype
R9524 T12219 T12218 arg1Of Ab,control
R9525 T12221 T12220 arg2Of from,were
R9526 T12222 T12221 arg2Of eBiosciences,from
R9527 T12225 T12223 arg1Of Ab,Rabbit
R9528 T12225 T12224 arg1Of Ab,anti-HOIP
R9529 T12225 T12226 arg1Of Ab,[
R9530 T12225 T12229 arg1Of Ab,was
R9531 T12227 T12226 arg2Of 6,[
R9532 T12228 T12226 arg3Of ],[
R9533 T12232 T12229 arg2Of gift,was
R9534 T12232 T12230 arg1Of gift,the
R9535 T12232 T12231 arg1Of gift,kind
R9536 T12232 T12233 arg1Of gift,of
R9537 T12236 T12233 arg2Of Eipper,of
R9538 T12236 T12234 arg1Of Eipper,Dr.
R9539 T12236 T12235 arg1Of Eipper,Betty
R9540 T12236 T12237 arg1Of Eipper,(
R9541 T12238 T12239 arg1Of University,of
R9542 T12238 T12243 arg1Of University,","
R9543 T12242 T12239 arg2Of Center,of
R9544 T12242 T12240 arg1Of Center,Connecticut
R9545 T12242 T12241 arg1Of Center,Health
R9546 T12243 T12237 arg2Of ",",(
R9547 T12244 T12243 arg2Of Farmington,","
R9548 T12244 T12245 arg1Of Farmington,","
R9549 T12246 T12245 arg2Of Connecticut,","
R9550 T12247 T12237 arg3Of ),(
R9551 T12250 T12248 arg1Of IgG,Goat
R9552 T12250 T12249 arg1Of IgG,anti-rat
R9553 T12250 T12252 arg1Of IgG,and
R9554 T12252 T12251 arg1Of and,","
R9555 T12252 T12256 arg1Of and,were
R9556 T12252 T12257 arg1Of and,from
R9557 T12255 T12252 arg2Of Abs,and
R9558 T12255 T12253 arg1Of Abs,HRP-labeled
R9559 T12255 T12254 arg1Of Abs,secondary
R9560 T12256 T12261 arg1Of were,","
R9561 T12257 T12256 arg2Of from,were
R9562 T12260 T12257 arg2Of Laboratories,from
R9563 T12260 T12258 arg1Of Laboratories,Jackson
R9564 T12260 T12259 arg1Of Laboratories,ImmunoResearch
R9565 T12265 T12262 arg1Of antibodies,Inc.
R9566 T12265 T12263 arg1Of antibodies,All
R9567 T12265 T12264 arg1Of antibodies,other
R9568 T12265 T12266 arg2Of antibodies,used
R9569 T12265 T12267 arg1Of antibodies,were
R9570 T12265 T12268 arg2Of antibodies,described
R9571 T12268 T12261 arg2Of described,","
R9572 T12268 T12267 arg2Of described,were
R9573 T12268 T12269 arg1Of described,previously
R9574 T12268 T12270 arg1Of described,[
R9575 T12271 T12270 arg2Of 6,[
R9576 T12272 T12270 arg3Of ],[
R9850 T12599 T12597 arg1Of gene,Somatic
R9851 T12599 T12598 arg1Of gene,cell
R9852 T12600 T12599 arg1Of targeting,gene
R9853 T12602 T12601 arg1Of generation,The
R9854 T12602 T12603 arg1Of generation,of
R9855 T12602 T12606 arg1Of generation,was
R9856 T12602 T12607 arg2Of generation,accomplished
R9857 T12605 T12603 arg2Of cells,of
R9858 T12605 T12604 arg1Of cells,HOIP-deficient
R9859 T12607 T12606 arg2Of accomplished,was
R9860 T12607 T12608 modOf accomplished,using
R9861 T12607 T12618 arg1Of accomplished,","
R9862 T12607 T12619 arg1Of accomplished,[
R9863 T12612 T12608 arg2Of approach,using
R9864 T12612 T12609 arg1Of approach,a
R9865 T12612 T12610 arg1Of approach,homologous
R9866 T12612 T12611 arg1Of approach,recombination
R9867 T12612 T12613 arg2Of approach,described
R9868 T12613 T12614 arg1Of described,previously
R9869 T12613 T12615 arg1Of described,[
R9870 T12616 T12615 arg2Of 7,[
R9871 T12617 T12615 arg3Of ],[
R9872 T12620 T12619 arg2Of 8,[
R9873 T12621 T12619 arg3Of ],[
R9874 T12622 T12623 arg1Of Segments,of
R9875 T12622 T12636 arg1Of Segments,were
R9876 T12622 T12637 arg2Of Segments,amplified
R9877 T12626 T12623 arg2Of sequence,of
R9878 T12626 T12624 arg1Of sequence,Rnf31
R9879 T12626 T12625 arg1Of sequence,gene
R9880 T12626 T12627 arg2Of sequence,used
R9881 T12627 T12628 arg1Of used,in
R9882 T12629 T12630 arg1Of the,targeting
R9883 T12630 T12628 arg2Of targeting,in
R9884 T12630 T12629 arg1Of targeting,the
R9885 T12631 T12630 arg2Of construct,targeting
R9886 T12631 T12632 arg1Of construct,(
R9887 T12634 T12632 arg2Of 1A,(
R9888 T12634 T12633 arg1Of 1A,Fig.
R9889 T12635 T12632 arg3Of ),(
R9890 T12637 T12636 arg2Of amplified,were
R9891 T12639 T12637 arg1Of PCR,amplified
R9892 T12639 T12638 arg2Of PCR,by
R9893 T12639 T12640 arg1Of PCR,from
R9894 T12643 T12640 arg2Of DNA,from
R9895 T12643 T12641 arg1Of DNA,A20.2J
R9896 T12643 T12642 arg1Of DNA,genomic
R9897 T12646 T12644 arg1Of primers,The
R9898 T12646 T12645 arg1Of primers,oligonucleotide
R9899 T12646 T12647 arg2Of primers,used
R9900 T12646 T12649 arg1Of primers,generate
R9901 T12646 T12657 arg1Of primers,were
R9902 T12646 T12658 arg1Of primers,5′-ttttctagagcggtggcttaagtgaccc-3′
R9903 T12646 T12660 arg1Of primers,5′-tattctagatgcagcatctgagaaagcaagc-3′
R9904 T12649 T12647 arg3Of generate,used
R9905 T12649 T12648 arg1Of generate,to
R9906 T12652 T12649 arg2Of flank,generate
R9907 T12652 T12650 arg1Of flank,the
R9908 T12652 T12651 arg1Of flank,5′
R9909 T12652 T12653 arg1Of flank,(
R9910 T12655 T12653 arg2Of bp,(
R9911 T12655 T12654 arg1Of bp,1224
R9912 T12656 T12653 arg3Of ),(
R9913 T12658 T12659 arg1Of 5′-ttttctagagcggtggcttaagtgaccc-3′,and
R9914 T12659 T12657 arg2Of and,were
R9915 T12660 T12659 arg2Of 5′-tattctagatgcagcatctgagaaagcaagc-3′,and
R9916 T12663 T12661 arg1Of flank,The
R9917 T12663 T12662 arg1Of flank,3′
R9918 T12663 T12664 arg1Of flank,(
R9919 T12666 T12664 arg2Of bp,(
R9920 T12666 T12665 arg1Of bp,6032
R9921 T12667 T12664 arg3Of ),(
R9922 T12668 T12663 arg1Of primers,flank
R9923 T12668 T12669 arg1Of primers,were
R9924 T12668 T12670 arg1Of primers,5′-aaaaccggtgtatgcttctttacgggagaaaaatattag-3′
R9925 T12668 T12672 arg1Of primers,5′-tataccggtatgaagccaaaggaacactgagag-3′
R9926 T12670 T12671 arg1Of 5′-aaaaccggtgtatgcttctttacgggagaaaaatattag-3′,and
R9927 T12671 T12669 arg2Of and,were
R9928 T12672 T12671 arg2Of 5′-tataccggtatgaagccaaaggaacactgagag-3′,and
R9929 T12675 T12673 arg1Of sites,Restriction
R9930 T12675 T12674 arg1Of sites,endonuclease
R9931 T12675 T12676 arg1Of sites,in
R9932 T12675 T12680 arg1Of sites,allowed
R9933 T12679 T12676 arg2Of primers,in
R9934 T12679 T12677 arg1Of primers,the
R9935 T12679 T12678 arg1Of primers,oligonucleotide
R9936 T12681 T12680 arg2Of insertion,allowed
R9937 T12681 T12682 arg1Of insertion,of
R9938 T12685 T12682 arg2Of products,of
R9939 T12685 T12683 arg1Of products,the
R9940 T12685 T12684 arg1Of products,PCR
R9941 T12685 T12686 arg1Of products,into
R9942 T12689 T12686 arg2Of vector,into
R9943 T12689 T12687 arg1Of vector,the
R9944 T12689 T12688 arg1Of vector,targeting
R9945 T12691 T12690 arg1Of cells,A20.2J
R9946 T12691 T12692 arg1Of cells,were
R9947 T12691 T12693 arg2Of cells,transfected
R9948 T12691 T12706 arg2Of cells,subcloned
R9949 T12693 T12694 arg1Of transfected,(
R9950 T12693 T12701 arg1Of transfected,with
R9951 T12693 T12705 arg1Of transfected,and
R9952 T12695 T12694 arg2Of by,(
R9953 T12696 T12695 arg2Of electroporation,by
R9954 T12696 T12697 arg1Of electroporation,[
R9955 T12698 T12697 arg2Of 7,[
R9956 T12699 T12697 arg3Of ],[
R9957 T12700 T12694 arg3Of ),(
R9958 T12702 T12703 arg1Of the,targeting
R9959 T12703 T12701 arg2Of targeting,with
R9960 T12703 T12702 arg1Of targeting,the
R9961 T12704 T12703 arg2Of construct,targeting
R9962 T12705 T12692 arg2Of and,were
R9963 T12706 T12705 arg2Of subcloned,and
R9964 T12706 T12707 arg1Of subcloned,in
R9965 T12708 T12707 arg2Of medium,in
R9966 T12708 T12709 arg1Of medium,containing
R9967 T12713 T12709 arg2Of sulfate,containing
R9968 T12713 T12710 arg1Of sulfate,600
R9969 T12713 T12711 arg1Of sulfate,µg/ml
R9970 T12713 T12712 arg1Of sulfate,G418
R9971 T12715 T12714 arg1Of recombination,Homologous
R9972 T12715 T12716 arg1Of recombination,in
R9973 T12715 T12719 arg1Of recombination,was
R9974 T12715 T12720 arg2Of recombination,detected
R9975 T12718 T12716 arg2Of clones,in
R9976 T12718 T12717 arg1Of clones,G418-resistant
R9977 T12720 T12719 arg2Of detected,was
R9978 T12720 T12726 arg1Of detected,","
R9979 T12720 T12727 arg1Of detected,as
R9980 T12722 T12720 arg1Of PCR,detected
R9981 T12722 T12721 arg2Of PCR,by
R9982 T12722 T12723 arg1Of PCR,of
R9983 T12725 T12723 arg2Of DNA,of
R9984 T12725 T12724 arg1Of DNA,genomic
R9985 T12728 T12727 arg2Of described,as
R9986 T12728 T12729 arg1Of described,[
R9987 T12730 T12729 arg2Of 7,[
R9988 T12731 T12729 arg3Of ],[
R9989 T12733 T12732 arg1Of primers,Oligonucleotide
R9990 T12733 T12734 arg2Of primers,used
R9991 T12733 T12737 arg1Of primers,were
R9992 T12733 T12738 arg1Of primers,5′-cttcctgatctcagctttaccgtcac-3′
R9993 T12733 T12753 arg1Of primers,5′-caatccatcttgttcagccat-3′
R9994 T12734 T12735 arg1Of used,for
R9995 T12736 T12735 arg2Of screening,for
R9996 T12738 T12739 arg1Of 5′-cttcctgatctcagctttaccgtcac-3′,(
R9997 T12738 T12752 arg1Of 5′-cttcctgatctcagctttaccgtcac-3′,and
R9998 T12740 T12741 arg1Of homologous,to
R9999 T12740 T12744 arg1Of homologous,;