| Id |
Subject |
Object |
Predicate |
Lexical cue |
| T13952 |
0-7 |
NN |
denotes |
Western |
| T13953 |
8-12 |
NN |
denotes |
blot |
| T13954 |
13-21 |
NN |
denotes |
analysis |
| T13955 |
22-31 |
VB |
denotes |
Following |
| T13956 |
32-43 |
NN |
denotes |
stimulation |
| T13957 |
43-44 |
-COMMA- |
denotes |
, |
| T13958 |
45-51 |
NN |
denotes |
Jurkat |
| T13959 |
52-59 |
NN |
denotes |
T-cells |
| T13960 |
60-64 |
VB |
denotes |
were |
| T13961 |
65-76 |
VB |
denotes |
centrifuged |
| T13962 |
77-79 |
IN |
denotes |
at |
| T13963 |
80-84 |
CD |
denotes |
1000 |
| T13964 |
85-86 |
SYM |
denotes |
× |
| T13965 |
87-88 |
NN |
denotes |
g |
| T13966 |
89-92 |
IN |
denotes |
for |
| T13967 |
93-94 |
CD |
denotes |
8 |
| T13968 |
95-98 |
NN |
denotes |
min |
| T13969 |
99-102 |
CC |
denotes |
and |
| T13970 |
103-108 |
VB |
denotes |
lysed |
| T13971 |
109-111 |
IN |
denotes |
on |
| T13972 |
112-115 |
NN |
denotes |
ice |
| T13973 |
116-119 |
IN |
denotes |
for |
| T13974 |
120-121 |
CD |
denotes |
2 |
| T13975 |
122-123 |
NN |
denotes |
h |
| T13976 |
124-129 |
VB |
denotes |
using |
| T13977 |
130-136 |
NN |
denotes |
sodium |
| T13978 |
137-146 |
NN |
denotes |
hydroxide |
| T13979 |
147-151 |
IN |
denotes |
with |
| T13980 |
152-155 |
DT |
denotes |
the |
| T13981 |
156-164 |
NN |
denotes |
addition |
| T13982 |
165-167 |
IN |
denotes |
of |
| T13983 |
168-169 |
DT |
denotes |
a |
| T13984 |
170-178 |
NN |
denotes |
protease |
| T13985 |
179-188 |
NN |
denotes |
inhibitor |
| T13986 |
189-197 |
NN |
denotes |
cocktail |
| T13987 |
198-199 |
-LRB- |
denotes |
( |
| T13988 |
199-204 |
NNP |
denotes |
Roche |
| T13989 |
204-205 |
-COMMA- |
denotes |
, |
| T13990 |
206-214 |
NNP |
denotes |
Mannheim |
| T13991 |
214-215 |
-RRB- |
denotes |
) |
| T13992 |
217-220 |
DT |
denotes |
The |
| T13993 |
221-226 |
NN |
denotes |
cells |
| T13994 |
227-231 |
VB |
denotes |
were |
| T13995 |
232-239 |
RB |
denotes |
further |
| T13996 |
240-251 |
VB |
denotes |
centrifuged |
| T13997 |
252-254 |
IN |
denotes |
at |
| T13998 |
255-259 |
CD |
denotes |
8000 |
| T13999 |
260-261 |
SYM |
denotes |
× |
| T14000 |
262-263 |
NN |
denotes |
g |
| T14001 |
263-264 |
-COMMA- |
denotes |
, |
| T14002 |
265-268 |
NN |
denotes |
4°C |
| T14003 |
269-272 |
IN |
denotes |
for |
| T14004 |
273-275 |
CD |
denotes |
10 |
| T14005 |
276-279 |
NN |
denotes |
min |
| T14006 |
280-283 |
CC |
denotes |
and |
| T14007 |
284-287 |
DT |
denotes |
the |
| T14008 |
288-300 |
NN |
denotes |
supernatants |
| T14009 |
301-305 |
VB |
denotes |
were |
| T14010 |
306-317 |
VB |
denotes |
transferred |
| T14011 |
318-320 |
TO |
denotes |
to |
| T14012 |
321-324 |
JJ |
denotes |
new |
| T14013 |
325-330 |
NN |
denotes |
tubes |
| T14014 |
332-343 |
JJ |
denotes |
Cytoplasmic |
| T14015 |
344-352 |
NN |
denotes |
proteins |
| T14016 |
353-354 |
-LRB- |
denotes |
( |
| T14017 |
354-356 |
CD |
denotes |
~8 |
| T14018 |
357-359 |
NN |
denotes |
μg |
| T14019 |
359-360 |
-RRB- |
denotes |
) |
| T14020 |
361-365 |
VB |
denotes |
were |
| T14021 |
366-375 |
VB |
denotes |
separated |
| T14022 |
376-378 |
IN |
denotes |
by |
| T14023 |
379-387 |
NN |
denotes |
SDS-PAGE |
| T14024 |
388-389 |
-LRB- |
denotes |
( |
| T14025 |
389-391 |
CD |
denotes |
10 |
| T14026 |
391-392 |
NN |
denotes |
% |
| T14027 |
392-393 |
-RRB- |
denotes |
) |
| T14028 |
394-402 |
VB |
denotes |
followed |
| T14029 |
403-405 |
IN |
denotes |
by |
| T14030 |
406-413 |
JJ |
denotes |
western |
| T14031 |
414-422 |
NN |
denotes |
blotting |
| T14032 |
423-428 |
VB |
denotes |
using |
| T14033 |
429-438 |
JJ |
denotes |
anti-IκBβ |
| T14034 |
438-439 |
-COMMA- |
denotes |
, |
| T14035 |
440-451 |
NN |
denotes |
phospho-PKC |
| T14036 |
452-453 |
-LRB- |
denotes |
( |
| T14037 |
453-456 |
NN |
denotes |
pan |
| T14038 |
456-457 |
-RRB- |
denotes |
) |
| T14039 |
457-458 |
-LRB- |
denotes |
( |
| T14040 |
458-459 |
SYM |
denotes |
ζ |
| T14041 |
460-466 |
NN |
denotes |
Thr410 |
| T14042 |
466-467 |
-RRB- |
denotes |
) |
| T14043 |
467-468 |
-LRB- |
denotes |
( |
| T14044 |
468-474 |
NN |
denotes |
190D10 |
| T14045 |
474-475 |
-RRB- |
denotes |
) |
| T14046 |
475-476 |
-COMMA- |
denotes |
, |
| T14047 |
477-487 |
NN |
denotes |
anti-Bcl10 |
| T14048 |
488-489 |
-LRB- |
denotes |
( |
| T14049 |
489-493 |
NNP |
denotes |
Cell |
| T14050 |
494-504 |
NNP |
denotes |
Signalling |
| T14051 |
505-515 |
NNP |
denotes |
Technology |
| T14052 |
515-516 |
-COMMA- |
denotes |
, |
| T14053 |
517-523 |
NNP |
denotes |
Boston |
| T14054 |
523-524 |
-RRB- |
denotes |
) |
| T14055 |
525-528 |
CC |
denotes |
and |
| T14056 |
529-539 |
NN |
denotes |
beta-actin |
| T14057 |
540-541 |
-LRB- |
denotes |
( |
| T14058 |
541-546 |
NNP |
denotes |
Abcam |
| T14059 |
546-547 |
-COMMA- |
denotes |
, |
| T14060 |
548-557 |
NNP |
denotes |
Cambridge |
| T14061 |
557-558 |
-RRB- |
denotes |
) |
| T14062 |
560-569 |
NN |
denotes |
Detection |
| T14063 |
570-573 |
VB |
denotes |
was |
| T14064 |
574-583 |
VB |
denotes |
performed |
| T14065 |
584-593 |
VB |
denotes |
following |
| T14066 |
594-604 |
NN |
denotes |
incubation |
| T14067 |
605-609 |
IN |
denotes |
with |
| T14068 |
610-614 |
NNP |
denotes |
ECL™ |
| T14069 |
615-626 |
NNP |
denotes |
Anti-rabbit |
| T14070 |
627-630 |
NN |
denotes |
IgG |
| T14071 |
630-631 |
-COMMA- |
denotes |
, |
| T14072 |
632-643 |
NN |
denotes |
horseradish |
| T14073 |
644-654 |
NN |
denotes |
peroxidase |
| T14074 |
655-661 |
VB |
denotes |
linked |
| T14075 |
662-667 |
JJ |
denotes |
whole |
| T14076 |
668-678 |
NN |
denotes |
antibodies |
| T14077 |
679-680 |
-LRB- |
denotes |
( |
| T14078 |
680-688 |
NNP |
denotes |
Amersham |
| T14079 |
689-700 |
NNP |
denotes |
Biosciences |
| T14080 |
700-701 |
-COMMA- |
denotes |
, |
| T14081 |
702-717 |
NNP |
denotes |
Buckinghamshire |
| T14082 |
717-718 |
-RRB- |
denotes |
) |
| T14083 |
719-722 |
CC |
denotes |
and |
| T14084 |
723-732 |
VB |
denotes |
developed |
| T14085 |
733-738 |
VB |
denotes |
using |
| T14086 |
739-743 |
NNP |
denotes |
ECL™ |
| T14087 |
744-751 |
NNP |
denotes |
Western |
| T14088 |
752-760 |
NNP |
denotes |
Blotting |
| T14089 |
761-770 |
NNP |
denotes |
Detection |
| T14090 |
771-779 |
NNP |
denotes |
Reagents |
| T14091 |
780-781 |
-LRB- |
denotes |
( |
| T14092 |
781-783 |
NNP |
denotes |
GE |
| T14093 |
784-794 |
NNP |
denotes |
Healthcare |
| T14094 |
794-795 |
-COMMA- |
denotes |
, |
| T14095 |
796-798 |
NNP |
denotes |
UK |
| T14096 |
798-799 |
-RRB- |
denotes |
) |
| R11254 |
T13954 |
T13952 |
arg1Of |
analysis,Western |
| R11255 |
T13954 |
T13953 |
arg1Of |
analysis,blot |
| R11256 |
T13956 |
T13955 |
arg2Of |
stimulation,Following |
| R11257 |
T13959 |
T13961 |
arg2Of |
T-cells,centrifuged |
| R11258 |
T13959 |
T13958 |
arg1Of |
T-cells,Jurkat |
| R11259 |
T13959 |
T13960 |
arg1Of |
T-cells,were |
| R11260 |
T13959 |
T13970 |
arg2Of |
T-cells,lysed |
| R11261 |
T13959 |
T13976 |
arg1Of |
T-cells,using |
| R11262 |
T13961 |
T13962 |
arg1Of |
centrifuged,at |
| R11263 |
T13961 |
T13969 |
arg1Of |
centrifuged,and |
| R11264 |
T13965 |
T13962 |
arg2Of |
g,at |
| R11265 |
T13965 |
T13963 |
arg1Of |
g,1000 |
| R11266 |
T13965 |
T13964 |
arg1Of |
g,× |
| R11267 |
T13965 |
T13966 |
arg1Of |
g,for |
| R11268 |
T13968 |
T13966 |
arg2Of |
min,for |
| R11269 |
T13968 |
T13967 |
arg1Of |
min,8 |
| R11270 |
T13969 |
T13955 |
arg1Of |
and,Following |
| R11271 |
T13969 |
T13957 |
arg1Of |
and,"," |
| R11272 |
T13969 |
T13960 |
arg2Of |
and,were |
| R11273 |
T13970 |
T13969 |
arg2Of |
lysed,and |
| R11274 |
T13970 |
T13971 |
arg1Of |
lysed,on |
| R11275 |
T13970 |
T13973 |
arg1Of |
lysed,for |
| R11276 |
T13970 |
T13976 |
modOf |
lysed,using |
| R11277 |
T13972 |
T13971 |
arg2Of |
ice,on |
| R11278 |
T13975 |
T13973 |
arg2Of |
h,for |
| R11279 |
T13975 |
T13974 |
arg1Of |
h,2 |
| R11280 |
T13976 |
T13979 |
arg1Of |
using,with |
| R11281 |
T13976 |
T13987 |
arg1Of |
using,( |
| R11282 |
T13978 |
T13976 |
arg2Of |
hydroxide,using |
| R11283 |
T13978 |
T13977 |
arg1Of |
hydroxide,sodium |
| R11284 |
T13981 |
T13979 |
arg2Of |
addition,with |
| R11285 |
T13981 |
T13980 |
arg1Of |
addition,the |
| R11286 |
T13981 |
T13982 |
arg1Of |
addition,of |
| R11287 |
T13986 |
T13982 |
arg2Of |
cocktail,of |
| R11288 |
T13986 |
T13983 |
arg1Of |
cocktail,a |
| R11289 |
T13986 |
T13984 |
arg1Of |
cocktail,protease |
| R11290 |
T13986 |
T13985 |
arg1Of |
cocktail,inhibitor |
| R11291 |
T13988 |
T13987 |
arg2Of |
Roche,( |
| R11292 |
T13988 |
T13989 |
arg1Of |
Roche,"," |
| R11293 |
T13988 |
T13990 |
arg1Of |
Roche,Mannheim |
| R11294 |
T13991 |
T13987 |
arg3Of |
),( |
| R11295 |
T13993 |
T13992 |
arg1Of |
cells,The |
| R11296 |
T13993 |
T13994 |
arg1Of |
cells,were |
| R11297 |
T13993 |
T13996 |
arg2Of |
cells,centrifuged |
| R11298 |
T13996 |
T13994 |
arg2Of |
centrifuged,were |
| R11299 |
T13996 |
T13995 |
arg1Of |
centrifuged,further |
| R11300 |
T13996 |
T13997 |
arg1Of |
centrifuged,at |
| R11301 |
T13996 |
T14006 |
arg1Of |
centrifuged,and |
| R11302 |
T14000 |
T13998 |
arg1Of |
g,8000 |
| R11303 |
T14000 |
T13999 |
arg1Of |
g,× |
| R11304 |
T14000 |
T14001 |
arg1Of |
g,"," |
| R11305 |
T14001 |
T13997 |
arg2Of |
",",at |
| R11306 |
T14002 |
T14001 |
arg2Of |
4°C,"," |
| R11307 |
T14002 |
T14003 |
arg1Of |
4°C,for |
| R11308 |
T14005 |
T14003 |
arg2Of |
min,for |
| R11309 |
T14005 |
T14004 |
arg1Of |
min,10 |
| R11310 |
T14008 |
T14007 |
arg1Of |
supernatants,the |
| R11311 |
T14008 |
T14009 |
arg1Of |
supernatants,were |
| R11312 |
T14008 |
T14010 |
arg2Of |
supernatants,transferred |
| R11313 |
T14010 |
T14006 |
arg2Of |
transferred,and |
| R11314 |
T14010 |
T14009 |
arg2Of |
transferred,were |
| R11315 |
T14010 |
T14011 |
arg1Of |
transferred,to |
| R11316 |
T14013 |
T14011 |
arg2Of |
tubes,to |
| R11317 |
T14013 |
T14012 |
arg1Of |
tubes,new |
| R11318 |
T14015 |
T14014 |
arg1Of |
proteins,Cytoplasmic |
| R11319 |
T14015 |
T14016 |
arg1Of |
proteins,( |
| R11320 |
T14015 |
T14020 |
arg1Of |
proteins,were |
| R11321 |
T14015 |
T14021 |
arg2Of |
proteins,separated |
| R11322 |
T14018 |
T14016 |
arg2Of |
μg,( |
| R11323 |
T14018 |
T14017 |
arg1Of |
μg,~8 |
| R11324 |
T14019 |
T14016 |
arg3Of |
),( |
| R11325 |
T14021 |
T14020 |
arg2Of |
separated,were |
| R11326 |
T14021 |
T14057 |
arg1Of |
separated,( |
| R11327 |
T14023 |
T14021 |
arg1Of |
SDS-PAGE,separated |
| R11328 |
T14023 |
T14022 |
arg2Of |
SDS-PAGE,by |
| R11329 |
T14023 |
T14024 |
arg1Of |
SDS-PAGE,( |
| R11330 |
T14023 |
T14028 |
arg2Of |
SDS-PAGE,followed |
| R11331 |
T14023 |
T14043 |
arg1Of |
SDS-PAGE,( |
| R11332 |
T14023 |
T14046 |
arg1Of |
SDS-PAGE,"," |
| R11333 |
T14026 |
T14024 |
arg2Of |
%,( |
| R11334 |
T14026 |
T14025 |
arg1Of |
%,10 |
| R11335 |
T14027 |
T14024 |
arg3Of |
),( |
| R11336 |
T14031 |
T14028 |
arg1Of |
blotting,followed |
| R11337 |
T14031 |
T14029 |
arg2Of |
blotting,by |
| R11338 |
T14031 |
T14030 |
arg1Of |
blotting,western |
| R11339 |
T14031 |
T14032 |
arg1Of |
blotting,using |
| R11340 |
T14035 |
T14032 |
arg2Of |
phospho-PKC,using |
| R11341 |
T14035 |
T14033 |
arg1Of |
phospho-PKC,anti-IκBβ |
| R11342 |
T14035 |
T14034 |
arg1Of |
phospho-PKC,"," |
| R11343 |
T14035 |
T14036 |
arg1Of |
phospho-PKC,( |
| R11344 |
T14035 |
T14039 |
arg1Of |
phospho-PKC,( |
| R11345 |
T14037 |
T14036 |
arg2Of |
pan,( |
| R11346 |
T14038 |
T14036 |
arg3Of |
),( |
| R11347 |
T14041 |
T14039 |
arg2Of |
Thr410,( |
| R11348 |
T14041 |
T14040 |
arg1Of |
Thr410,ζ |
| R11349 |
T14042 |
T14039 |
arg3Of |
),( |
| R11350 |
T14044 |
T14043 |
arg2Of |
190D10,( |
| R11351 |
T14045 |
T14043 |
arg3Of |
),( |
| R11352 |
T14047 |
T14048 |
arg1Of |
anti-Bcl10,( |
| R11353 |
T14047 |
T14055 |
arg1Of |
anti-Bcl10,and |
| R11354 |
T14051 |
T14048 |
arg2Of |
Technology,( |
| R11355 |
T14051 |
T14049 |
arg1Of |
Technology,Cell |
| R11356 |
T14051 |
T14050 |
arg1Of |
Technology,Signalling |
| R11357 |
T14051 |
T14052 |
arg1Of |
Technology,"," |
| R11358 |
T14053 |
T14052 |
arg2Of |
Boston,"," |
| R11359 |
T14054 |
T14048 |
arg3Of |
),( |
| R11360 |
T14055 |
T14046 |
arg2Of |
and,"," |
| R11361 |
T14056 |
T14055 |
arg2Of |
beta-actin,and |
| R11362 |
T14058 |
T14057 |
arg2Of |
Abcam,( |
| R11363 |
T14058 |
T14059 |
arg1Of |
Abcam,"," |
| R11364 |
T14058 |
T14060 |
arg1Of |
Abcam,Cambridge |
| R11365 |
T14061 |
T14057 |
arg3Of |
),( |
| R11366 |
T14062 |
T14063 |
arg1Of |
Detection,was |
| R11367 |
T14062 |
T14064 |
arg2Of |
Detection,performed |
| R11368 |
T14064 |
T14063 |
arg2Of |
performed,was |
| R11369 |
T14064 |
T14065 |
arg1Of |
performed,following |
| R11370 |
T14066 |
T14065 |
arg2Of |
incubation,following |
| R11371 |
T14066 |
T14067 |
arg1Of |
incubation,with |
| R11372 |
T14069 |
T14068 |
arg1Of |
Anti-rabbit,ECL™ |
| R11373 |
T14070 |
T14067 |
arg2Of |
IgG,with |
| R11374 |
T14070 |
T14069 |
arg1Of |
IgG,Anti-rabbit |
| R11375 |
T14073 |
T14072 |
arg1Of |
peroxidase,horseradish |
| R11376 |
T14073 |
T14074 |
arg1Of |
peroxidase,linked |
| R11377 |
T14073 |
T14084 |
arg2Of |
peroxidase,developed |
| R11378 |
T14074 |
T14083 |
arg1Of |
linked,and |
| R11379 |
T14076 |
T14074 |
arg2Of |
antibodies,linked |
| R11380 |
T14076 |
T14075 |
arg1Of |
antibodies,whole |
| R11381 |
T14076 |
T14077 |
arg1Of |
antibodies,( |
| R11382 |
T14079 |
T14077 |
arg2Of |
Biosciences,( |
| R11383 |
T14079 |
T14078 |
arg1Of |
Biosciences,Amersham |
| R11384 |
T14079 |
T14080 |
arg1Of |
Biosciences,"," |
| R11385 |
T14081 |
T14080 |
arg2Of |
Buckinghamshire,"," |
| R11386 |
T14082 |
T14077 |
arg3Of |
),( |
| R11387 |
T14083 |
T14063 |
modOf |
and,was |
| R11388 |
T14083 |
T14071 |
arg1Of |
and,"," |
| R11389 |
T14084 |
T14083 |
arg2Of |
developed,and |
| R11390 |
T14085 |
T14084 |
arg3Of |
using,developed |
| R11391 |
T14090 |
T14085 |
arg2Of |
Reagents,using |
| R11392 |
T14090 |
T14086 |
arg1Of |
Reagents,ECL™ |
| R11393 |
T14090 |
T14087 |
arg1Of |
Reagents,Western |
| R11394 |
T14090 |
T14088 |
arg1Of |
Reagents,Blotting |
| R11395 |
T14090 |
T14089 |
arg1Of |
Reagents,Detection |
| R11396 |
T14090 |
T14091 |
arg1Of |
Reagents,( |
| R11397 |
T14093 |
T14091 |
arg2Of |
Healthcare,( |
| R11398 |
T14093 |
T14092 |
arg1Of |
Healthcare,GE |
| R11399 |
T14093 |
T14094 |
arg1Of |
Healthcare,"," |
| R11400 |
T14095 |
T14094 |
arg2Of |
UK,"," |
| R11401 |
T14096 |
T14091 |
arg3Of |
),( |
